CN102643752A - Inorganic culture solution for microalgae abnormal fermentation - Google Patents
Inorganic culture solution for microalgae abnormal fermentation Download PDFInfo
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- CN102643752A CN102643752A CN2012101397669A CN201210139766A CN102643752A CN 102643752 A CN102643752 A CN 102643752A CN 2012101397669 A CN2012101397669 A CN 2012101397669A CN 201210139766 A CN201210139766 A CN 201210139766A CN 102643752 A CN102643752 A CN 102643752A
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Abstract
The invention discloses an inorganic culture solution for microalgae abnormal fermentation, which belongs to the technical field of biological fermentation. The inorganic culture solution comprises sodion ions, potassium ions, magnesium ions, calcium ions, chloride ions and phosphoric acid dihydro ions. The inorganic culture solution is simple and easy to practical, low in cost and friendly in production environment, schizochytrium limacinum frond cultivated through the inorganic culture solution is high in fat mass, and percentage composition of docosahexaenoic acid (DHA) in total fat mass is high.
Description
Technical field:
The invention belongs to the biologics preparing technical field, relate to a kind of inorganic nutrient solution, relate in particular to the inorganic nutrient solution of a kind of little algae difference fermentation.
Background technology:
Split the kettle algae and claim that again schizochytrium limacinum (Schizochytrium limacinum) is a kind of marine microorganism, it has abundant and colory grease, and high person's fat content can reach more than 50%, mostly is pufas, and particularly DHA content is abundant; Therefore, splitting the kettle algae and be not only biofuel and edible oil potential raw material, also is the raw material of health care medicine industry, is the essential bait of the sea life young simultaneously, and the mass-producing cultivation of splitting the kettle algae has become emerging biological industry.
Research shows, splits the kettle algae and in fresh water, can not grow, and seawater is the essential material in its vital process, cultivates site limitation at coastal region; In order to break through geological restraint, many researchs substitute nature seawater with the seawater of artificial preparation and have produced good effect, but no matter are the nature seawater or the seawater of artificial preparation, and the inorganic salt concentration of its composition is widely different; Some sea area sea water salinity reaches about 35 ‰, and some sea area sea water salinity is 28 ‰, and salinity is lower in the marine site, river mouth, and artificial seawater has than big-difference equally, and its proportioning of the artificial seawater of different plant produced is also different; Mostly the optimization of the culture condition such as raw material composition, pH value, oxygen level, temperature of nutritive substance such as C/N ratio, the carbon source nitrogenous source of counterincision kettle algae in existing research; Influence research for inorganic ion concentration counterincision kettle algae is less; But different inorganic ion concentration can influence the living weight that splits the kettle algae, greasy accumulation and DHA content; The variation of inorganic salt concentration influences culture effect equally, and therefore good inorganic nutrient solution is most important to little algae stable high yield; Seawater is to sea life; And remarkable be osmotic pressure influence, but the various compositions in the seawater are participated in halobiontic vital movement, even more high sea life such as fish, shrimp etc. different results can occur under the identical inorganic salt proportioning of osmotic pressure condition of different; Some can only be survived a couple of days; Some can long life, and some sexual gland can not maturation etc., and the single-cell sea biology is higher to the degree of dependence of seawater.All kinds of mineral ions are the combined effect of collective to organism in the seawater; Study the influence that different inorganic ion concentration counterincision kettle algaes grow up; A kind of difference that little algae such as kettle algae cultivates inorganic nutrient solution that ferments that is suitable for splitting is provided, and it is imperative to cultivate with the mass-producing that adapts to little algae under the situation of breaking through geological restraint.
Summary of the invention:
The present invention is intended to overcome the influence research barren defective of to inorganic ion concentration little algae being cultivated in the prior art, seeks to design the inorganic nutrient solution of a kind of little algae difference fermentation.
In order to realize the foregoing invention purpose, ion that the inorganic nutrient solution of little algae difference fermentation that the present invention relates to comprises and mass concentration (unit: restrain every liter) thereof as follows:
Sodium ion (Na
+) 4.00-4.40 preferred 4.10,4.20; Potassium ion (K
+) 0.80-1.00 preferred 0.90; Mg ion (Mg
2+) 1.50-2.00 preferred 1.60,1.80; Cl ions (Cl
-) 7.80-8.10 preferred 7.90,8.00; Dihydrogen phosphate ions (H
2PO
4 -) 2.00-3.00 preferred 2.20,2.40,2.60; Or comprise sulfate ion (SO again
4 2-), calcium ion (Ca
2+), and bicarbonate ion (HCO
3 -) be used to regulate pH value; The sodium ion that the present invention relates to, potassium ion, mg ion, cl ions, dihydrogen phosphate ions, sulfate ion, calcium ion, bicarbonate ion are taken from sodium-chlor, Repone K, potassium primary phosphate, sal epsom, calcium chloride, Calcium hydrogen carbonate.
The present invention is compared with prior art simple, and cost is low, and production environment is friendly; Be used for cultivating when splitting the kettle algae, turn out to split kettle algae frond fat quantity high, the percentage composition of DHA is high in total fat quantity.
Embodiment:
Through specific embodiment the present invention is described further below.
Embodiment 1:
1 liter of inorganic nutrient solution of little algae difference fermentation of present embodiment preparation, proportioning and method for making are following:
With sodium-chlor 10.17 grams, sal epsom 7.50 grams, potassium primary phosphate 2.80 grams, calcium chloride 2.54 grams are dissolved in 500 ml waters and mix, and dilute with water is settled to 1 liter, stirs and promptly gets the inorganic nutrient solution of little algae difference fermentation.
Embodiment 2:
1 liter of inorganic nutrient solution of little algae difference fermentation of present embodiment preparation, proportioning and method for making are following:
With sodium-chlor 11.19 grams, sal epsom 10.00 grams, potassium primary phosphate 4.21 grams, calcium chloride 2.05 grams are dissolved in 500 ml waters and mix, and dilute with water is settled to 1 liter, stirs and promptly gets the inorganic nutrient solution of little algae difference fermentation.
Embodiment 3:
The influence of present embodiment research sodium ion and growth of cl ions counterincision kettle algae and frond lipid content; With Subow (1941) artificial seawater of 50% concentration as standard recipe; Add carbon nitrogen nutrition source; On this basis, the concentration of adjustment sodium ion and cl ions is observed and is split the variation of DHA quality percentage composition in fat mass percentage composition, the TL in kettle algal biomass amount percentage composition, the frond; Obtain best salinity concentration then, the result sees table 1.
The influence of growth of table 1 NaCl concentration (unit: restrain each hundred milliliters) counterincision kettle algae and frond lipid content
It is favourable by the higher NaCl concentration of the visible appropriateness of table 1 frond to be grown, but fat and DHA accumulation are caused disadvantageous effect, and the analysis-by-synthesis mass concentration is optimum between each hundred milliliters of 0.9-1.1 grams.
Embodiment 4:
The influence of present embodiment research mg ion counterincision kettle algae growth and frond lipid content; As standard recipe, add carbon nitrogen nutrition source with Subow (1941) artificial seawater of 50% concentration, on this basis, use MgSO
4.7H
2O is the concentration of raw material adjustment mg ion, observes and splits the variation of DHA quality percentage composition in fat mass percentage composition, the TL in kettle algal biomass amount percentage composition, the frond, obtains best salinity concentration then, and the result sees table 2.
Table 2 MgSO
4The influence of growth of concentration (unit: restrain each hundred milliliters) counterincision kettle algae and frond lipid content
Use identical Mg
2+The MgCl of concentration
2Substitute MgSO
4Draw similar result, explain that the growth of mg ion counterincision kettle algae is in close relations with the accumulation of frond fat, and SO4
2-The negatively charged ion influence is little.
Embodiment 5:
The influence of present embodiment research potassium ion counterincision kettle algae growth and frond lipid content; As standard recipe, add carbon nitrogen nutrition source with Subow (1941) artificial seawater of 50% concentration, on this basis, make the raw material adjustment potassium concentration of potassium ion with KCl, because KCl content in seawater is lower, the Cl among the KCl
-Can not impact the result; The variation of DHA quality percentage composition in fat mass percentage composition, the TL in kettle algal biomass amount percentage composition, the frond is split in observation, obtains best salinity concentration then, and the result sees table 3.
The influence of growth of table 3 KCl concentration (unit: restrain each hundred milliliters) counterincision kettle algae and frond lipid content
Table 3 data declaration potassium ion changes the frond growth influential in the scope of normal seawater, and it is accumulated in 0.08-0.10 to fat and DHA and restrains in the scope of each hundred milliliters of mass concentration positive result is arranged.
Because the content of calcium ion in natural sea-water is minimum, so calcium is little to the algae grows influence.
Embodiment 6:
The influence of present embodiment research phosphonium ion counterincision kettle algae growth and frond lipid content; As standard recipe, add carbon nitrogen nutrition source with Subow (1941) artificial seawater of 50% concentration, on this basis, adjust the concentration of phosphonium ion as the phosphorus source with dihydrogen phosphate; The variation of DHA quality percentage composition in fat mass percentage composition, the TL in kettle algal biomass amount percentage composition, the frond is split in observation, obtains best salinity concentration then, and the result sees table 4.
The influence of growth of table 4 phosphate ion concentration (unit: restrain each hundred milliliters) counterincision kettle algae and frond lipid content
Embodiment 7:
Present embodiment is summed up fatty effect of accumulation in each ionic concn counterincision kettle algae growth of embodiment 4-7 and the frond, draws and splits the inorganic nutrient solution optimal ion concentration of kettle algae difference fermentation, and is as shown in table 5.
Table 5 splits the inorganic nutrient solution optimal ion concentration of kettle algae difference fermentation
| The ion title | Unit | Mass concentration | The ion title | Unit | Mass concentration |
| Sodium ion Na + | g/L | 4.00-4.40 | Cl ions Cl - | g/L | 7.80-8.10 |
| Potassium ion K + | g/L | 0.80-1.00 | Sulfate radical SO 4 2- | g/L | Influence not obvious |
| Mg ion Mg 2+ | g/L | 1.50-2.00 | Hydrogen phosphate H 2PO 4 - | g/L | 2.00-3.00 |
| Calcium ion Ca 2+ | g/L | 0.2-0.3 | Bicarbonate radical HCO 3 - | g/L | Looking PH regulates |
The present invention and Copenhagen water contrast, wherein K
+Concentration is higher than seawater, and phosphoric is considered the favourable function of frond is had a mind to add HCO
3 -Consider the extra interpolation of pH balance of nutrient chemical.
Claims (6)
1. inorganic nutrient solution of little algae difference fermentation; It is characterized in that comprising following ion and mass concentration thereof: every liter of sodium ion 4.00-4.40 gram; Every liter of potassium ion 0.80-1.00 gram; Mg ion 1.50-2.00 gram liter, every liter of cl ions 7.80-8.10 gram, every liter of dihydrogen phosphate ions 2.00-3.00 gram.
2. the inorganic nutrient solution of little algae difference fermentation according to claim 1; It is characterized in that comprising following ion and mass concentration thereof: every liter of sodium ion 4.10 gram, every liter of potassium ion 0.90 gram, every liter of mg ion 1.80 gram; Every liter of cl ions 8.00 gram, every liter of dihydrogen phosphate ions 2.40 gram.
3. the inorganic nutrient solution of little algae difference fermentation according to claim 1; It is characterized in that comprising following ion and mass concentration thereof: every liter of sodium ion 4.20 gram, every liter of potassium ion 0.90 gram, every liter of mg ion 1.60 gram; Every liter of cl ions 7.90 gram, every liter of dihydrogen phosphate ions 2.60 gram.
4. according to the inorganic nutrient solution of each described little algae difference fermentation of claim 1-3, it is characterized in that also comprising sulfate ion.
5. according to the inorganic nutrient solution of each described little algae difference fermentation of claim 1-3, it is characterized in that also comprising bicarbonate ion.
6. according to the inorganic nutrient solution of each described little algae difference fermentation of claim 1-3, it is characterized in that also comprising calcium ion.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103775745A (en) * | 2012-10-24 | 2014-05-07 | 苏州新达电扶梯部件有限公司 | Cable frame at bottom of car |
| CN108753622A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of based on molasses give up mash metal ion improve single needle algae fat content |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831387A (en) * | 2010-04-16 | 2010-09-15 | 厦门大学 | Fully-synthetic culture medium, preparation method thereof and method for culturing Schizochytrium sp. by using same |
| CN101886044A (en) * | 2010-07-17 | 2010-11-17 | 厦门大学 | Preparation method of DHA ( |
| CN102199541A (en) * | 2011-04-29 | 2011-09-28 | 国家海洋局第三海洋研究所 | Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and fermentation method thereof |
-
2012
- 2012-05-08 CN CN2012101397669A patent/CN102643752A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831387A (en) * | 2010-04-16 | 2010-09-15 | 厦门大学 | Fully-synthetic culture medium, preparation method thereof and method for culturing Schizochytrium sp. by using same |
| CN101886044A (en) * | 2010-07-17 | 2010-11-17 | 厦门大学 | Preparation method of DHA ( |
| CN102199541A (en) * | 2011-04-29 | 2011-09-28 | 国家海洋局第三海洋研究所 | Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and fermentation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 陈丽珠等: "流加培养裂殖壶菌发酵生产二十二碳六烯酸", 《厦门大学学报（自然科学版）》 * |
| 魏萍等: "裂殖壶菌发酵生产DHA研究进展", 《食品工业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103775745A (en) * | 2012-10-24 | 2014-05-07 | 苏州新达电扶梯部件有限公司 | Cable frame at bottom of car |
| CN108753622A (en) * | 2018-05-30 | 2018-11-06 | 昆明理工大学 | A method of based on molasses give up mash metal ion improve single needle algae fat content |
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Application publication date: 20120822 |