CN102199541A - Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and fermentation method thereof - Google Patents
Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and fermentation method thereof Download PDFInfo
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Abstract
The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g/L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.
Description
Technical field
What the present invention relates to a kind of high yield DHA splits algae strain of kettle algae and fermentation process thereof.
Background technology
Docosahexenoic acid (DHA) mainly is present in human brain grey matter and the retina, be to keep the metabolic indispensable fatty acid composition of human body normal physiological, in infant's brain and eyesight growth, have very important physiological action, and can treat cardiovascular disorder, improve body immunity etc.DHA causes the great interest of people because of its distinctive physiological regulation function and is widely used.
The tradition source of DHA is fish oil, but the polyunsaturated fatty acid that extracts from fish oil (PUFAs) exists the output shakiness, yield is low, cost is high and contain other problems such as ω-6PUFAs, and along with the growing tension of fishing resources, oneself can't satisfy the growing market requirement traditional DHA resource.Therefore, the exploitation DHA newborn resource oneself become new research focus.Microbial fermentation is produced DHA becomes one of focus of domestic and international research with its special advantages, wherein utilizes little algae to cultivate production DHA and has bigger advantage and produce potential.
Little algae has that growth cycle is short, reproduction speed is fast, plasticity-is strong, nutritive factor is required characteristics such as simple, and part can reach high density fermentation by the control of biological engineering method and culture condition and cultivates.Found at present to be rich in polyunsaturated fatty acid (PUFAs) in multiple little algae, as split kettle algae, Crypthecodinium cohnii etc.Little algae is the initial production person of polyunsaturated fatty acid in the marine food chain, many saturated fatty acids DHA in some frustule, EPA content are higher, its relative content is up to the 5%-6% of dry cell weight, and contained polyunsaturated fatty acid kind is more simple, the separation of carrying out single component is purified relatively easy, thereby to utilize marine microalgae to produce polyunsaturated fatty acid be a very promising commercial field.Little algae oil has been widely used in fields such as medicine, food, makeup, feed because of being rich in polyunsaturated fatty acid.
It is abundant to split in the kettle frustule DHA content, and other unsaturated fatty acid content is very low.This algae is safe in utilization, and it has carried out a series of security detection to white mouse, rabbit humans such as Hammond, does not find any toxic side effect.The security of splitting the kettle algae has obtained the approval of U.S. food and medicine portion (Food and Drug Administration).The research that kettle algae fermentation culture is produced DHA is split in existing more utilization both at home and abroad at present, but has the not high problem of output mostly.T.Yokocki etc. adopt several kinds of carbon source and nitrogenous source counterincision kettle algae Schizochytriumlimacinum SR21 to be optimized cultivation in shaking bottle, and the DHA production peak that obtains is 4g/L.People such as Kw Fan are that kettle algae (Schizochytrium mangroves) is split in carbon, nitrogenous source cultivation with glucose and yeast extract, and the output of DHA only is 2.79g/L.The output of DHA is not very high in more than cultivating, and this also has direct relation except outside the Pass having with selected algae strain with culture process.
Summary of the invention
What the purpose of this invention is to provide a kind of high yield DHA splits algae strain of kettle algae and fermentation process thereof.
The invention provides and split kettle algae Schizochytrium sp.TIO1101, its deposit number is CGMCCNo.4603.
Be used for the described fermention medium that splits kettle algae TIO1101, the preparation method is as follows: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium primary phosphate 2-4g, sal epsom 1-3g, micro-complex liquid 8-11ml and VITAMIN complex liquid 4-8ml are settled to 1L with 0.5 * seawater; Described 0.5 * seawater mixes 1 parts by volume seawater and obtains with 1 parts by volume water.
In the described fermention medium: described glucose is that 120g, described yeast extract are that 20g, described peptone are that 15g, described potassium primary phosphate are that 3g, described sal epsom are that 2g, described micro-complex liquid are that 10ml, described VITAMIN complex liquid are 5ml.
The preparation method of described micro-complex liquid is specific as follows: Na
2EDTA 6.0g, FeCl
36H
2O 0.29g, H
3BO
36.84g, MnCl
24H
2O 0.86g, ZnCl
20.06g, CoCl
26H2O 0.026g, NiSO
46H
2O 0.052g, CuSO
45H
2O 0.002g, NaMoO
42H
2O 0.005g, water is settled to 1L.
The preparation method of described VITAMIN complex liquid is specific as follows: VITMAIN B1 (thiamin) 100mg, and vitamin H (biotin) 0.5mg, vitamin B12 (cyanocobalamin) 0.5mg, water is settled to 1L.
The present invention also protects a kind of described method of splitting kettle algae TIO1101 of cultivating, and is at 25-30 ℃, pH 5.5-7.5, condition under cultivate the described kettle algae TIO1101 that splits.In the described cultural method, temperature can be 25 ℃, and pH can be 6.0.Described cultivation is preferably carried out in described fermention medium.The described inoculum density of kettle algae TIO1101 in described fermention medium that split can be 1.0 * 10
6Individual/mL.
The described kettle algae TIO1101 that splits can be used for preparing grease and/or docosahexenoic acid.
The present invention also protects a kind of method for preparing grease and/or docosahexenoic acid, is the described kettle algae TIO1101 that splits of fermentation, obtains grease and/or docosahexenoic acid.The parameter of described fermentation can be: 25-30 ℃, and pH 5.5-7.5.The parametric optimization of described fermentation is 25 ℃, and pH 6.0, dissolved oxygen 10%.Can adopt the rotating speed of 200-400rpm in the described fermentation, preferably adopt the rotating speed of 300rpm.The time of described fermentation specifically can be 96 hours.Described fermentation is preferably carried out in described fermention medium.The described inoculum density of kettle algae TIO1101 in described fermention medium that split can be 1.0 * 10
6Individual/mL.In the process of described fermentation, can carry out batch feeding.For every 6L fermention medium, described batch feeding specifically can be: begin stream from moment of fermentation 12h and add glucose solution and (be made up of glucose and water, the concentration of glucose is 50g/L) and yeast extract paste solution (form by yeast extract paste and water, the concentration of yeast extract paste is 10g/L) until the moment of fermentation 50h, the flow velocity of glucose solution is that the flow velocity of 50mL/h, yeast extract paste solution is 20mL/h.
Behind the fermentation 72h, the biomass, fat content and the DHA that split kettle algae TIO1101 account for total lipid content and are respectively 120g/L, 54.3% and 51.4%, and the dried frond of every 100g can obtain 54.3g grease, 27.9gDHA, has development and application values.
Beneficial effect of the present invention: (1) separation has obtained to split kettle algae TIO1101; (2) realized splitting the high-density heterotrophic fermentation of kettle algae TIO1101; (3) this fermentation condition obtains down splits that kettle algae TIO1101 cell is carried in the grease DHA content height and composition is comparatively simple.
Description of drawings
Fig. 1 is for splitting kettle algae TIO1101 18S rRNA gene PCR electrophorogram.
Fig. 2 is for splitting kettle algae TIO1101 18S rRNA gene evolution tree.
Fig. 3 splits the variation diagram of kettle algae TIO1101 dry cell weight with fermentation time.
Fig. 4 is the color atlas that splits after the esterification of kettle algae TIO1101 oil sample.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique among the following embodiment if no special instructions, is ordinary method.Used test materials among the following embodiment if no special instructions, is to buy from routine biochemistry reagent shop and obtains.Quantitative test in following examples all is provided with repeated experiments three times, results averaged.
Seawater: take from Xiamen sea area.
The preparation method of trace element complex liquid: Na
2EDTA 6.0g, FeCl
36H
2O 0.29g, H
3BO
36.84g, MnCl
24H
2O 0.86g, ZnCl
20.06g, CoCl
26H2O 0.026g, NiSO
46H
2O 0.052g, CuSO
45H
2O0.002g, NaMoO
42H
2O 0.005g, distilled water is supplied 1L.
The preparation method of VITAMIN complex liquid: VITMAIN B1 (thiamin) 100mg, vitamin H (biotin) 0.5mg, vitamin B12 (cyanocobalamin) 0.5mg, distilled water is supplied 1L.
The preparation method of fermention medium: glucose 120g, yeast extract 20g, peptone 15g, potassium primary phosphate 3g, sal epsom 2g, micro-complex liquid 10ml, VITAMIN complex liquid 5ml are settled to 1L with 0.5 * seawater (with the seawater of 1 times of fresh water dilution).
Embodiment 1, split separation and the evaluation of kettle algae TIO1101
One, splits the separation (Pollen Pini fish method) of kettle algae TIO1101
Get the corruption fallen leaves in the mangrove forest of Sha Men Yuandang Ya lake, be cut into diameter and be about the sequin of 1.5cm, clean with the aseptic seawater that contains Streptomycin sulphate and each 1g/L of penicillin, be inoculated on the solid separating plate, each plate 3-5 sheet, add the aseptic seawater of about 3mL and a small amount of aseptic Pollen Pini on flat board, cultivate 4-5d down at 28 ℃, the picking Pollen Pini, observation adheres to the culture of growth on Pollen Pini, the Pollen Pini that adheres to culture is transferred to the separation of ruling on the new flat board again, till obtaining pure growth.With a strain algae called after TIO1101 who obtains.
Two, split the evaluation of kettle algae TIO1101
1, algae falls and the cellular form observation
Culture is observed fall form and observe its cellular form by film-making under powerful microscope of the algae that grows after cultivating 5d on the separation and purification flat board.Split that kettle algae algae falls to being faint yellow, opaque, the edge is irregular, its vegetative cell is an elliposoidal, the about 7-13 μ of diameter m, the growth of row binary fission.
2, Molecular Identification
(1) extracting genome DNA
Adopt the cracking process of improvement to extract total DNA.
Get fermented liquid 5mL in centrifuge tube, the centrifugal 5min of 8000r/min, centrifugal collection frond under the similarity condition after sterile distilled water washs a time.Place aseptic mortar to add an amount of liquid nitrogen grinding fresh frond.Frond after the grinding is transferred in the centrifuge tube, adds 2.4ml lysate (0.25mol/L Tris-Cl pH 8.2,0.1mol/L EDTA pH 8.0,2%SDS, 0.1mol/L NaCl), cracking 40min behind the mixing.Add isopyknic phenol: chloroform: primary isoamyl alcohol (25: 24: 1) extraction, isopropanol precipitating, washing with alcohol.The DNA that carries adds the TE solution dissolving of 50 μ l10mmol/L, and-20 ℃ of refrigerators are preserved.
(2) 18S rRNA amplification, order-checking
Amplimer is: forward Pf:5 '-CCAACCTGGTTGATCCTGCCAGTA-3 ';
Reverse Pr:5 '-CCTTGTTACGACTTCACCTTCCTCT-3.
50 μ L PCR reaction systems are formed: 10 * PCR buffer (5 μ L), dNTP (4 μ L), forward primer (1 μ L), reverse primer (1 μ L), TaKaRa Taq (0.2 μ L, 1.25U), template DNA (2 μ L, 0.6～1.5 μ g), distilled water complements to 50 μ L.
The pcr amplification condition: 95 ℃ of pre-sex change 5min, 95 ℃ of sex change 30s, 55 ℃ of annealing 30s, 72 ℃ are extended 1min, 30 circulations, last 72 ℃ are extended 10min.The sepharose of employing 1% is seen Fig. 1 to PCR product electrophoresis detection, produces the 18S rRNA gene that size is about 1700bp.，
Pcr amplification product is connected with the T carrier, checks order in Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.Sequence total length 1707bp (seeing the sequence table 1 of sequence table).
(3) Phylogenetic Analysis
The 18S rRNA gene order that order-checking is obtained compares by Blast program and GenBank amplifying nucleic acid data, utilizes ClustalX 1.8 and Mega2 software building phylogenetic tree, carries out Phylogenetic Analysis.The result shows that this algae strain 18S rRNA gene and Schizochytrium mangrovei have the homology more than 99%, utilize the systematic evolution tree of ClustalX 1.8 and Mega2 software building to see Fig. 2, systematic evolution tree shows that it is one that this algae strain and Schizochytrium mangrovei gather, and sibship is nearest.
Fall and the result of cellular form and Molecular Identification according to algae, TIO1101 belongs to and splits kettle algae (Schizochytriumsp.).Split kettle algae Schizochytrium sp.TIO1101 and be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center (abbreviation CGMCC on 02 23rd, 2011, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), preserving number is CGMCC No.4603.Split kettle algae Schizochytrium sp.TIO1101CGMCC No.4603 abbreviation and split kettle algae TIO1101.
Embodiment 2, split the high-density heterotrophic fermentation of kettle algae TIO1101
One, fermentation
In the 10L fermentor tank, add the 6L fermention medium, add 0.6L then and split kettle algae TIO1101 bacterium liquid, make and split the concentration of kettle algae TIO1101 in substratum and be about 1.0 * 10
6Individual/mL; Fermentation culture conditions is: 25 ℃ of temperature, and pH value 6.0, rotating speed 300rpm (200-400rpm all can), dissolved oxygen 10% is cultivated 96h; Carrying out 10L fermentor tank batch feeding cultivates: begin stream from moment of fermentation 12h and add glucose solution and (be made up of glucose and water, the concentration of glucose is 50g/L) and yeast extract paste solution (form by yeast extract paste and water, the concentration of yeast extract paste is 10g/L) until the moment of fermentation 50h, the flow velocity of glucose solution is that the flow velocity of 50mL/h, yeast extract paste solution is 20mL/h.
Two, biomass, grease and DHA content detection
1, biomass
Get the fermented liquid detection of biological amount (in the frond dry cell weight of collecting) of different fermentations time in the step 1, method is as follows: get the 100mL fermented liquid and pack in the centrifuge tube of weighing in advance, the centrifugal 10min collecting precipitation of 8000r/min, after sterile distilled water cleaning 3 times, vacuum-drying is weighed to constant weight, is dried frond.Split kettle algae dry weight (i.e. the weight of dried frond) variation such as Fig. 3 with fermentation time.Behind the fermentation culture 72h, splitting the kettle algae, to obtain high-biomass be 140g/L.
2, grease
The dried frond that step 1 the is obtained back of pulverizing is adopted Soxhlet extraction process (GB5009.6-85) extracting grease and is weighed.Fat content in the dried frond is higher, reaches 54.3%.
3、DHA
Get the grease obtained sample of 0.1g extracting and place 10ml centrifuge tube with cover, add the 1mL ether: (ether: normal hexane is 2: 1 to the normal hexane mixed solution; Volume ratio), shake up the KOH/CH that adds 0.8mol/L behind the 15min
3OH solution 2mL shakes up back 50 ℃ of reaction 15min, adds water to 10mL scale place along wall, and standing demix is got supernatant liquor and carried out the capillary gas chromatography analysis.
The parameter that capillary gas chromatography is analyzed: Varian GC3800 gas chromatograph, fid detector, the CP-sil5CB capillary column (0.32mm * 0.25 μ m * 30m); Carrier gas is a nitrogen, and flow velocity is 30.0mL/min; Injector temperature is 250 ℃, and detector temperature is 280 ℃; Column temperature employing program is given birth to temperature: 160 ℃ of initial temperatures (keeping 3min), be raised to 230 ℃ with 6 ℃/min speed, and keep 8min.Adopting methyl margarate is that interior mark carries out the inner mark method ration analysis.
The typical curve function is Y=1.4648X-0.0087, and correlation coefficient r is 0.9999.Wherein Y-axis is represented the ratio of analyte peak size and interior mark peak size.X-axis represent advance analyte amount and advance in the ratio of scalar.In mark methyl margarate and split kettle algae oil sample color atlas and see Fig. 4.
The result shows that split the content that kettle algae DHA accounts for total fat is 51.4%.
The dried frond of every 100g can obtain the 54.3g grease, obtains 27.9gDHA.
Claims (10)
1. split kettle algae Schizochytrium sp.TIO1101, its deposit number is CGMCC No.4603.
2. be used for the described fermention medium that splits kettle algae TIO1101 of claim 1, the preparation method is as follows: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium primary phosphate 2-4g, sal epsom 1-3g, micro-complex liquid 8-11ml and VITAMIN complex liquid 4-8ml are settled to 1L with 0.5 * seawater; Described 0.5 * seawater mixes 1 parts by volume seawater and obtains with 1 parts by volume water.
3. fermention medium as claimed in claim 2 is characterized in that: described glucose is that 120g, described yeast extract are that 20g, described peptone are that 15g, described potassium primary phosphate are that 3g, described sal epsom are that 2g, described micro-complex liquid are that 10ml, described VITAMIN complex liquid are 5ml.
4. as claim 2 or 3 described fermention mediums, it is characterized in that:
The preparation method of described micro-complex liquid is as follows: Na
2EDTA 6.0g, FeCl
36H
2O 0.29g, H
3BO
36.84g, MnCl
24H
2O 0.86g, ZnCl
20.06g, CoCl
26H2O 0.026g, NiSO
46H
2O 0.052g, CuSO
45H
2O 0.002g, NaMoO
42H
2O 0.005g, water is settled to 1L;
The preparation method of described VITAMIN complex liquid is as follows: VITMAIN B1 100mg, and vitamin H 0.5mg, vitamin B12 0.5mg, water is settled to 1L.
5. cultivating the described method of splitting kettle algae TIO1101 of claim 1 for one kind, is at 25-30 ℃, pH 5.5-7.5, condition under cultivate the described kettle algae TIO1101 that splits.
6. method as claimed in claim 5 is characterized in that: described temperature is 25 ℃, and described pH is 6.0; Described cultivation is preferably carried out in claim 2 or 3 or 4 described fermention mediums.
7. claim 1 is described splits the application of kettle algae TIO1101 in preparation grease and/or docosahexenoic acid.
8. a method for preparing grease and/or docosahexenoic acid is the described kettle algae TIO1101 that splits of fermentation claim 1, obtains grease and/or docosahexenoic acid.
9. method as claimed in claim 8 is characterized in that: the parameter of described fermentation is: 25-30 ℃, and pH5.5-7.5; The parametric optimization of described fermentation is 25 ℃, and pH 6.0, dissolved oxygen 10%.
10. method as claimed in claim 8 or 9, it is characterized in that: described fermentation is carried out in claim 3 or 4 or 5 described fermention mediums.
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-
2011
- 2011-04-29 CN CN 201110111247 patent/CN102199541B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 《中国油脂》 20070630 周林等 Schizochytrium sp.发酵生产DHA培养基的优化 32-34 1-10 第32卷, 第6期 * |
| 《微生物学通报》 20060831 周茂洪等 1株裂殖弧菌(Schizochytrium sp.)的分离鉴定 48-51 1-10 第33卷, 第4期 * |
| 《江苏食品与发酵》 20080630 叶磊等 微生物发酵法生产DHA 25-28 1-10 , * |
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| AU2017391757B2 (en) * | 2017-01-03 | 2018-11-08 | Sea-Nergy Pty Ltd | Hydrogen production |
| CN106987528A (en) * | 2017-05-31 | 2017-07-28 | 厦门汇盛生物有限公司 | One plant production docosahexaenoic acid bacterium and its application |
| CN106987528B (en) * | 2017-05-31 | 2020-05-05 | 厦门汇盛生物有限公司 | Bacterium for producing docosahexaenoic acid and application thereof |
| CN108949580A (en) * | 2018-05-18 | 2018-12-07 | 武汉藻优生物科技有限公司 | A kind of high DHA content splits pot algae and its application |
| CN110800871A (en) * | 2019-11-07 | 2020-02-18 | 湖北欣和生物科技有限公司 | Application of schizochytrium limacinum powder in improving DHA content in ruminant milk |
| CN110846232A (en) * | 2019-12-04 | 2020-02-28 | 南京工业大学 | Strain capable of producing DHA through high-temperature fermentation and application thereof |
| CN110846232B (en) * | 2019-12-04 | 2021-08-03 | 南京工业大学 | Strain capable of producing DHA through high-temperature fermentation and application thereof |
| CN114940947A (en) * | 2022-04-29 | 2022-08-26 | 深圳古他生物科技有限公司 | DHA-producing schizochytrium GT-D1 and application thereof, DHA-rich grease and preparation method thereof |
| CN114940947B (en) * | 2022-04-29 | 2024-03-29 | 深圳古他生物科技有限公司 | Schizochytrium limacinum GT-D1 capable of producing DHA, application thereof, DHA-rich grease and preparation method thereof |
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