CN102641161B - A kind of artificial blood vessel with composite structure and dynamic preparation method thereof - Google Patents
A kind of artificial blood vessel with composite structure and dynamic preparation method thereof Download PDFInfo
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- CN102641161B CN102641161B CN201210121091.5A CN201210121091A CN102641161B CN 102641161 B CN102641161 B CN 102641161B CN 201210121091 A CN201210121091 A CN 201210121091A CN 102641161 B CN102641161 B CN 102641161B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/507—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials for artificial blood vessels
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- Vascular Medicine (AREA)
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- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
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Abstract
The present invention relates to a kind of artificial blood vessel with composite structure and dynamic preparation method thereof, this artificial blood vessel comprises: the pipe base (1) of weaving base and nanofiber element coating (9); Preparation method comprises: the pipe base (1) being obtained artificial blood vessel by nonwoven techniques such as woven, knitting, braiding or water thorns, then described pipe base is placed in bacteria cellulose bio-reactor, with support (2) through pipe base (1), space is left between support (2) and pipe base (1), after high-temperature sterilization, pour aseptic fermention medium, access bacteria cellulose production bacterium seed liquor in the reactor into, after dynamic cultivation in pipe base outer surface one layer of nano-cellulose film, to obtain final product. The strength and extension property of the thin vessels radial-axial of the present invention is good, the thin requirement meeting small-caliber vascular of tube wall, tube wall good leak tightness, it is not necessary to pre-blood coagulation; The preparation method of the present invention is simple, and nanometer bacteria cellulose coating biology consistency and biomechanical property are good.
Description
Technical field
The invention belongs to artificial blood vessel and preparation field thereof, in particular to a kind of artificial blood vessel with composite structure and dynamic preparation method thereof.
Background technology
Current heavy caliber blood vessel graft has achieved good effect in clinical application, but common small-bore artificial blood vessel does not almost have antithrombotic.
Intellectual Property Right in China system shows, existing intellecture property project reduces the formation of thrombus by blood vessel carries out the method for finishing at present, if number of patent application is " composite vascular prosthesis that a kind of fabric strengthens " middle composite vascular prosthesis proposing to be made up of anticoagulation layer, textile reinforcement and elastic bonding layer of 200610166568.6, improve artificial blood vessel patency rate to a certain extent; But need elastic bonding layer to be connected between textile reinforcement with anticoagulation layer, cause tube wall thicker, be unfavorable for being applied on small-bore artificial blood vessel, and its complex structure, preparation difficulty, coating amount is uncontrollable.
Common artificial blood vessel for medical material is generally hydrophobic material surface free energy height, and material itself easily interacts with plasma proteins, formation mural thrombus.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of artificial blood vessel with composite structure and its preparation method, the internal diameter of this artificial blood vessel can��6mm, the strength and extension property of blood vessel radial-axial is good, the thinner requirement more meeting small-caliber vascular of tube wall; This preparation method is simple, and the adhesive power between coating and pipe base is good, not easily peels off.
A kind of artificial blood vessel with composite structure of the present invention, this artificial blood vessel comprises: the pipe base of weaving base and nanofiber element coating, the internal diameter of described pipe base can be less than or equal 6mm.
The structural pipe wall of described pipe base is woven, knitting, braiding or non-weaves, and its hole is relatively big and Stability Analysis of Structures.
The material of described pipe base is the medical non-degradable polymer fiber material with biocompatibility.
The preparation method of a kind of artificial blood vessel with composite structure of the present invention, comprising:
The pipe base of artificial blood vessel is obtained by woven, knitting, braiding or the nonwoven techniques such as acupuncture, water acupuncture manipulation, then described pipe base is placed in bacteria cellulose bio-reactor, with support through pipe base, space is left between support and pipe base, after high-temperature sterilization, pour in the reactor aseptic fermention medium, access bacteria cellulose produce bacterium seed liquor, dynamic cultivation after 1-7 days in pipe base outer surface one layer of nano-cellulose film, obtain artificial blood vessel with composite structure.
In described bacteria cellulose bio-reactor, pipe base rotates along with the rotation of rotating disk, as shown in Figure 1.
In described bacteria cellulose bio-reactor, pipe base moves up and down, as shown in Figure 2.
Described support is bar-shaped support.
It is that acetic bacteria belongs to (Acetobactersp.) that described bacteria cellulose produces bacterium, gluconobacter suboxydans belongs to (Gluconobactersp.), glyconic acid genus acetobacter (Gluconacetobactersp.), glucose oxidation and bacillus (Gluconobacteroxydans), rhizobium (Rhizobiumsp.), sarcina belongs to (Sarcinasp.), Rhodopseudomonas (Pseudomounassp.), achromobacter (Achromobactersp.), Alcaligenes (Alcaligenessp.), aerobacter (Aerobactersp.), Azotobacter (Azotobactersp.), Agrobacterium (Agrobacteriumsp.), pseudomonas cepacia (Seudomonascepacia), campylobacter jejuni (Campylobacterjejuni), acetobacter xylinum (Acetobacterxylinum) or tea fungus (kombucha).
The design of the present invention:
1) material
According to service requirements, the materials'use of pipe base has the medical non-degradable polymer fiber material of biocompatibility.
The nano-cellulose that nanofiber element coating use fermentation using bacteria obtains.
2) pipe blank structure design
The tube wall of pipe base takes woven, knitting, braiding or non-woven structure, by adopting proper yarn arrays and proportioning, add the processing mode corresponded, it is achieved on a kind of artificial blood vessel tube wall with relatively macrovoid but the woven fabric structure of Stability Analysis of Structures.
3.) nano-cellulose overlay film
Basic fundamental route is as follows:
Pipe base is placed in bacteria cellulose bio-reactor (as depicted in figs. 1 and 2), with bar-shaped support through pipe base, space is left between bar-shaped support and pipe base, after high-temperature sterilization, pour aseptic fermention medium in the reactor into, access bacteria cellulose produces bacterium seed liquor, after being placed under certain temperature dynamic cultivation certain time in pipe base outer surface one layer of nano-cellulose film.
The pipe base of weaving base is immersed in nutrient solution by the present invention, and the nano-cellulose that bacterium produces forms cellulosic coating to run through structural growth on weaving base pipe base, the final artificial blood vessel forming bacterial fibers element coating.
Nanometer bacteria cellulose is direct growth on weaving base pipe base, forms the coating running through structure, overcomes the problem of above-mentioned small-caliber vascular complex structure, preparation difficulty; Pipe base is individual layer weaving base, and in order to meet nanometer bacteria cellulose coating needs, pipe base has the performance of macrovoid and Stability Analysis of Structures, and the thin vessels tube wall obtained is thinner; Nanometer bacteria cellulose coating is by bacterial secretory, with run through form growth adhere to weaving base pipe base on, by control growth time, growth conditions etc. control its growth amount and direction, so coating amount is controlled and coating evenly; Nanofiber element coating has higher biocompatibility and excellent biomechanical property, and be that hydrophilic material surface free energy is low, material itself not easily interacts with plasma proteins, prevents mural thrombus from being formed, it is to increase the patency rate of small-bore artificial blood vessel.
Useful effect
(1) the pipe base of the present invention is individual layer weaving base, has the performance of macrovoid and Stability Analysis of Structures, and the strength and extension property of the thin vessels radial-axial obtained is good, the thinner requirement more meeting small-caliber vascular of tube wall;
(2) nanometer bacteria cellulose direct growth on weaving base pipe base, forms the coating running through structure, and structure is simple, preparation is easy, and the adhesive power between coating and pipe base is good, not easily peels off;
(3) nanometer bacteria cellulose coating is secreted by cellulosic bacteria, adhere on weaving base pipe base with the form growth run through, by amount and the direction of control its growth of control such as growth time, growth conditions, so coating amount and homogeneity are artificially controlled;
(4) nanometer bacteria cellulose coating has higher biocompatibility and excellent biomechanical property, and is that hydrophilic material surface free energy is low, and material itself not easily interacts with plasma proteins, effectively prevents mural thrombus from being formed.
(5) the artificial blood vessel tube wall good leak tightness of nanofiber element coating, it is not necessary to pre-blood coagulation, Final finishing operation can suitably simplify.
Accompanying drawing explanation
Fig. 1 is the device schematic diagram (rotation of pipe base) of the present invention's bacteria cellulose bio-reactor used;
Fig. 2 is the device schematic diagram (up and down motion of pipe base) of the present invention's bacteria cellulose bio-reactor used;
Fig. 3 is the stereoscan photograph of viscose fiber pipe base coated with nano Mierocrystalline cellulose in embodiment 1;
Fig. 4 is the organization chart of the tubular tissue of leno weave in embodiment 1;
Fig. 5 is the tube wall of cellulosic coating in embodiment 1;
Fig. 6 is the organization chart of latitude plain stitch in embodiment 2;
Wherein 1-pipe base, 2-support (bar-shaped), 3-rotating disk, 4-substratum liquid level, 5-water-bath, 6-ground warp, 7-doup end, 8-weft yarn, 9-bacterial fibers element coating.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further. Limit the scope of the invention it will be understood that these embodiments are only not used in for illustration of the present invention. In addition it will be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application's appended claims limited range equally.
The invention is not restricted to the bacteria cellulose bio-reactor rotating or moving up and down shown in Fig. 1 or Fig. 2, the bacteria cellulose bio-reactor of dynamic cultivation.
Embodiment 1
The material of pipe base adopts the viscose glue bright yarn of 50D/12f; It is three strands through weft structure to compile in collaboration with; Pipe base tissue employing basic organization is the tubular tissue (weaving with weft yarn 8 by ground 6, doup end 7 as shown in Figure 5) of leno weave. Fabric parameter: be 400*100 through close * filling density (root/10cm), folding footpath (mm) is 10, and total quantity of warps (root) is 40.
Weave figure is shown in Fig. 4. Select the Mierocrystalline cellulose of acetobacter xylinum fermentation that pipe base is carried out coating. In bio-reactor (Fig. 1 and 2) fermentation culture 1-7 days, the small-bore artificial blood vessel of final obtained bacterial fibers element coating.
Fermentating culturing process:
(1) spawn culture: acetobacter xylinum (Acetobacterxylinum) is accessed 300mL liquid nutrient medium (in every 1L water, N.F,USP MANNITOL 20g, peptone 3g, yeast extract 5g, pH3.0,121 DEG C of sterilizing 20min; Or glucose 200g, yeast extract 5g, Tryptones 5g, citric acid 115g, Na2HPO42.7g, water 1L, pH7.5,121 DEG C of sterilizing 20min) spread cultivation, in 20 DEG C, 100r/min when shaking table cultivate or after quiescent culture 12h for subsequent use;
(2) fermentation of bacteria cellulose composite vascular prosthesis is standby: transferring to containing the liquid nutrient medium producing bacterial strain of step (1) being prepared is equipped with in the bio-reactor securing blank tube material, then pipe base with the rotating speed of 5-60rpm rotate (Fig. 1) or move up and down (Fig. 2) carry out disturbance cultivation, in 30 DEG C of water-baths 5, dynamic cultivation is after 1-7 days, in pipe base the artificial blood vessel of outer surface nanometer bacteria cellulose, the SEM photograph of material is shown in Fig. 3.
Embodiment 2
The material of pipe base adopts the viscose glue bright yarn of 50D/12f; Yarn structure is three strands and compiles in collaboration with; Tissue employing basic organization is the knit tubular tissue of latitude plain stitch. Fabric parameter: be 35*55 through close * filling density (individual/5cm), syringe diameter (mm) is 6.
Weave figure is shown in Fig. 6. Select the Mierocrystalline cellulose that glucose oxidation and bacillus (Gluconobacteroxydans) ferments that pipe base is carried out coating. In bio-reactor (Fig. 1 and 2) fermentation culture 1-7 days, the small-bore artificial blood vessel of final obtained bacterial fibers element coating. Electromicroscopic photograph and Fig. 3 result of the artificial blood vessel material of coated with nano Mierocrystalline cellulose are similar.
Fermentating culturing process:
(1) spawn culture: glucose oxidation and bacillus (Gluconobacteroxydans) is accessed 300mL liquid nutrient medium (in every 1L water, N.F,USP MANNITOL 20g, peptone 3g, yeast extract 5g, pH3.0,121 DEG C of sterilizing 20min; Or glucose 200g, yeast extract 5g, Tryptones 5g, citric acid 115g, Na2HPO42.7g, water 1L, pH7.5,121 DEG C of sterilizing 20min) spread cultivation, in 20 DEG C, 100r/min when shaking table cultivate or after quiescent culture 12h for subsequent use;
(2) fermentation of bacteria cellulose composite vascular prosthesis is standby: transferring to containing the liquid nutrient medium producing bacterial strain of step (1) being prepared is equipped with in the bio-reactor securing blank tube material, then pipe base with the rotating speed of 5-60rpm rotate (Fig. 1) or move up and down (Fig. 2) carry out disturbance cultivation, in 30 DEG C of water-baths 5, dynamic cultivation is after 1-7 days, in pipe base the artificial blood vessel of outer surface nanometer bacteria cellulose.
Embodiment 3
The yarn of pipe base adopts the viscose glue bright yarn of 50D/12f; Tissue adopts basic organization for rope volume tubular tissue. Fabric parameter: spool number is 32, core cylinder diameter (mm) is 6.
Select the Mierocrystalline cellulose that tea fungus (kombucha) ferments that pipe base is carried out coating. In bio-reactor (Fig. 1 and 2) fermentation culture 1-7 days, the small-bore artificial blood vessel of final obtained bacterial fibers element coating. Electromicroscopic photograph and Fig. 3 result of the artificial blood vessel material of coated with nano Mierocrystalline cellulose are similar.
Fermentating culturing process:
(1) spawn culture: the inoculum size that tea fungus (kombucha) contains bacterium BC film by access 5 diameter 0.5cm disks is accessed 300mL liquid seed culture medium (in every 1L water, green tea 5g, glucose 10, peptone 3g, yeast extract 5g, pH3.0, pasteurization 30min; In every 1L water, glucose 100g, peptone 3g, yeast extract 5g, pH7.5,121 DEG C of sterilizing 20min) spread cultivation, in 25 DEG C, 150r/min when shaking table cultivate or after quiescent culture 24h for subsequent use;
(2) fermentation of bacteria cellulose composite vascular prosthesis is standby: transferring to containing the liquid nutrient medium producing bacterial strain of step (1) being prepared is equipped with in the bio-reactor securing blank tube material, then pipe base with the rotating speed of 5-60rpm rotate (Fig. 1) or move up and down (Fig. 2) carry out disturbance cultivation, in 30 DEG C of water-baths 5, dynamic cultivation is after 1-7 days, in pipe base the artificial blood vessel of outer surface nanometer bacteria cellulose.
Claims (7)
1. an artificial blood vessel with composite structure, this artificial blood vessel is made up of the pipe base (1) of base of weaving and nanofiber element coating (9); The structural pipe wall of described pipe base (1) is woven, knitting or braiding, and its hole is relatively big and Stability Analysis of Structures; Fabric parameter: be 400 �� 100/10cm through close �� filling density, folding footpath is 10mm, and total quantity of warps is 40 or fabric parameter: be 35 �� 55/5cm through close �� filling density, and syringe diameter is 6mm.
2. a kind of artificial blood vessel with composite structure according to claim 1, it is characterised in that: the material of described pipe base (1) is the medical polymer filamentary material with biocompatibility.
3. a preparation method for artificial blood vessel with composite structure as claimed in claim 1, comprising:
The pipe base (1) of artificial blood vessel is obtained by woven, knitting or braiding, then described pipe base is placed in bacteria cellulose bio-reactor, with support (2) through pipe base (1), space is left between support (2) and pipe base (1), after high-temperature sterilization, pour aseptic fermention medium, access bacteria cellulose production bacterium seed liquor in the reactor into, dynamic cultivation after 1-7 days in pipe base outer surface one layer of nano-cellulose film, to obtain final product.
4. the preparation method of a kind of artificial blood vessel with composite structure according to claim 3, it is characterised in that: in described bacteria cellulose bio-reactor, pipe base (1) rotates along with the rotation of rotating disk (3).
5. the preparation method of a kind of artificial blood vessel with composite structure according to claim 3, it is characterised in that: in described bacteria cellulose bio-reactor, pipe base (1) moves up and down.
6. the preparation method of a kind of artificial blood vessel with composite structure according to claim 3, it is characterised in that: described support (2) is bar-shaped or tubular bracket.
7. the preparation method of a kind of artificial blood vessel with composite structure according to claim 3, it is characterised in that: it is that acetic bacteria belongs to (Acetobactersp.) that described bacteria cellulose produces bacterium, gluconobacter suboxydans belongs to (Gluconobactersp.), glyconic acid genus acetobacter (Gluconacetobactersp.), glucose oxidation and bacillus (Gluconobacteroxydans), rhizobium (Rhizobiumsp.), sarcina belongs to (Sarcinasp.), Rhodopseudomonas (Pseudomounassp.), achromobacter (Achromobactersp.), Alcaligenes (Alcaligenessp.), aerobacter (Aerobactersp.), Azotobacter (Azotobactersp.), Agrobacterium (Agrobacteriumsp.), pseudomonas cepacia (Seudomonascepacia), campylobacter jejuni (Campylobacterjejuni), acetobacter xylinum (Acetobacterxylinum) or tea fungus (kombucha).
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103341208B (en) * | 2013-07-09 | 2015-05-06 | 钟春燕 | Preparation method of hollow biological cellulose tube |
| CN104689382A (en) * | 2015-03-18 | 2015-06-10 | 东华大学 | Braiding-reinforced nano-fiber small-caliber intravascular stent, and preparation and application thereof |
| CN104963094A (en) * | 2015-07-08 | 2015-10-07 | 江南大学 | Non-woven cloth prepared by composite fibers by means of bacterial cellulose produced by microorganisms and preparation method thereof |
| CN109880140B (en) * | 2019-01-30 | 2022-09-27 | 东华大学 | Alkali-shrinkable bacteria nano cellulose tube and preparation method and application thereof |
| CN111388760B (en) * | 2020-03-25 | 2022-03-15 | 华东交通大学 | Small blood vessel stent with nano fibers oriented along circumferential direction and preparation method |
| CN114159624B (en) * | 2021-11-24 | 2022-09-02 | 山东黄河三角洲纺织科技研究院有限公司 | Coating method of woven artificial blood vessel and artificial blood vessel |
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| EP0396344A2 (en) * | 1989-04-28 | 1990-11-07 | Ajinomoto Co., Inc. | Hollow microbial cellulose, process for preparation thereof, and artificial blood vessel formed of said cellulose |
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