CN102633707A - Method for preparing natural 5-hydroxy tryptamine hydrochloride by utilizing banana peel - Google Patents
Method for preparing natural 5-hydroxy tryptamine hydrochloride by utilizing banana peel Download PDFInfo
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- CN102633707A CN102633707A CN2012101055820A CN201210105582A CN102633707A CN 102633707 A CN102633707 A CN 102633707A CN 2012101055820 A CN2012101055820 A CN 2012101055820A CN 201210105582 A CN201210105582 A CN 201210105582A CN 102633707 A CN102633707 A CN 102633707A
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Abstract
5-hydroxytryptamine is an indole derivative and can participate in various central nervous activities, so that people generate cheerful mood. The invention discloses a method for preparing natural 5-hydroxy tryptamine hydrochloride by utilizing banana peel. The method comprises the following preparation procedures: cleaning the banana peel, drying and crushing the banana peel; carrying out ultrasonic auxiliary water bath extraction; carrying out column chromatography separation; concentrating an extracting solution; leaching by ethanol; and crystallizing. The method disclosed by the invention is simple and feasible in process, scientific and rational in procedure, rigorous in working procedure, low in cost, easy to implement, small in energy consumption, high in extracting rate and high in product purity. According to the invention, only alcohol and ultrasonic auxiliary water are used for refining, so the pollution of an organic solvent is avoided; a source of natural 5-hydroxy tryptamine hydrochloride is provided, and the method can be widely applied to the field of food processing, so market requirements are met; and a new way is provided for comprehensive utilization of the banana peel, and the method has wide market development prospect.
Description
Technical field
The present invention relates to a kind of method of utilizing Pericarpium Musae to prepare natural serotonin hydrochloride, specifically is a kind of method of utilizing the auxiliary water-bath extraction of UW to prepare the serotonin hydrochloride, belongs to the processing of farm products field.
Background technology
Serotonin is a kind of indole derivatives, is made up of indoles and ethamine two portions, and molecular formula is C10H12N20, English name: 5-hydroxy tryptamine, be called for short 5-HT, and be prevalent in the animal vegetable tissue.Serotonin is separated from serum in nineteen forty-one the earliest, so claim thrombotonin again.Go out pure serotonin in the general strong and Abbott's laboratory chemosynthesis of the U.S. subsequently, its nourishing function has been obtained approval subsequently.People confirm at present: serotonin can be participated in multiple nervus centralis activity as a kind of neurotransmitter, can produce the courier of joyful mood, almost has influence on each aspect of brain activity: from regulating mood, strengthening energy and memory etc.Depression, impulsion, excessive drinking, suicide, attack and act of violence take place in crowd that the serotonin level is lower more easily.Quickening along with the fierceness and the rhythm of life of social competition; The pressure that people experienced is also increasing; Along and reduce pressure, medicine happy and that regulate spirit more and more receives people's attention, thereby serotonin medical field at home and abroad becomes a research focus.Along with the fast continual development of healthcare products, the also increasing healthcare products that are used for of serotonin, nowadays serotonin is used widely in America and Europe's a lot of large-scale health care company, and some domestic health care companies also begin to pay attention to the research to it.
Bibliographical information, serotonin is widespread in nature, except the blood and enteron aisle of animal, the serotonin that all exists content not wait in the various plants such as batrachians, hornet, scorpion and pineapple and banana.At present commercially available natural serotonin mainly comes from African plant Ghana seed, and its quantity is few, and cost is high, and can not satisfy market demand far away.And commercially available serotonin all exists with the form of its verivate, and its highly purified natural serotonin hydrochloride does not then have commercially available.
Banana is the torrid zone and subtropics characteristic fruit, also is the fruit of volume of trade maximum in the world.Whole world banana ultimate production reaches 7,000 ten thousand tons, and China successfully reached 1,000 ten thousand tons in 2010.The research that has has found that not only comprehensive nutrition of banana; And nourishing function is outstanding; Contain tens kinds of mineral substance such as protein, fat, sugar and K, Ca, Mg, S, Fe, Zn of rich in banana and the Pericarpium Musae thereof, also be rich in flavones, polysaccharide and serotonin.Banana is acknowledged as " happy food " owing to be rich in serotonin! Here it is, and people usually see the reason that the sportsmen eats banana at match rest periods such as tennis, shuttlecocks.Pericarpium Musae accounts for 40% of banana gross weight, and the whole world has 4,000,000 tons of Pericarpium Musaes to go out of use every year, does not only utilize and causes environmental pollution on the contrary.It is fully feasible utilizing Pericarpium Musae to extract serotonin, but does not at home and abroad also appear in the newspapers at present.
Disclose a kind of method of from Pericarpium Musae, extracting serotonin, not only can turn waste into wealth, increase the benefit of banana peasant and processing enterprise.Also expanded the raw material sources of natural serotonin greatly, significant to satisfying people to the demand of natural serotonin.
Summary of the invention
The objective of the invention is to overcome the few problem in serotonin source, and disclose a kind of method of utilizing Pericarpium Musae to prepare natural serotonin hydrochloride,, make high-purity natural serotonin hydrochloride through optimization to serotonin extraction process in the Pericarpium Musae.
The present invention accomplishes through the following steps preparation:
A. Pericarpium Musae cleans: gather Pericarpium Musae and clean, be cut into foursquare little bulk;
B. drying and crushing: the block Pericarpium Musae oven dry 24h that under 70 ℃ of-80 ℃ of conditions of temperature, will cut; The Pericarpium Musae of oven dry is pulverized, crossed 5 mesh sieves, obtain Pericarpium Musae dry powder;
C. ultrasonic auxiliary water-bath lixiviate: Pericarpium Musae dry powder is added water according to 1: 6 solid-liquid ratio of weight part mix; Utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate; UW lixiviate power is that 80W-100W, temperature are that 70 ℃-80 ℃, time are 2-3.5h, obtains extractive substance;
D. column chromatography for separation: it is the separator column of 30mm that the above-mentioned extractive substance that obtains is placed diameter, with D918Na type resin with the flow velocity of 2.8BV/h-3.8BV/h through the resin chromatography column, the ethanol with 70% at room temperature carries out the wash-out separation and purification, obtains extracting solution;
E. extracting solution concentrates: extracting solution is concentrated under the 600-650mmHg negative pressure, and 75 ℃-80 ℃ of thickening temperatures are 60-70 with the extracting solution concentrating under reduced pressure to Biax, obtain liquid concentrator;
F. ethanol lixiviate: extract 2-4 time 75 ℃ of-80 ℃ of condition refluxed with 65%-70% ethanol, add the alcohol hydrochloric acid mixed solution in the liquid concentrator, the volume ratio of liquid concentrator and alcohol hydrochloric acid mixed solution is 1: 1;
G. crystallization: the liquid concentrator after the ethanol lixiviate is cooled to 0 ℃, and crystallization appears in container bottom, and xln is dried to permanent quality under 90 ℃ of-100 ℃ of conditions, promptly obtains serotonin hydrochloride finished product.
The present invention is simple for process, and flow process is scientific and reasonable, and operation is rigorous, and cost is low, and is easy to implement, and energy consumption is little, high efficiency, and extraction yield is high, and product purity is high.Only refine with alcohol and UW auxiliary water; Avoided the pollution of organic solvent; A kind of source of natural serotonin hydrochloride not only is provided, can be widely used in food processing field, satisfied the market requirement; And, has the wide development prospect for the comprehensive utilization of Pericarpium Musae provides a new approach.
Embodiment
Be described in further detail the present invention below in conjunction with specific embodiment, employed TP is ordinary method like no specified otherwise among the following embodiment, and employed material, reagent etc. like no specified otherwise, are the reagent and the material that can obtain from commercial sources.
Embodiment one:
A. Pericarpium Musae cleans: gather Pericarpium Musae and clean, be cut into 1.5cm * 1.5cm square shape;
B. drying and crushing: under 75 ℃ of conditions of temperature, dry 24h, the Pericarpium Musae of oven dry is pulverized, cross 5 mesh sieves, obtain Pericarpium Musae dry powder;
C. ultrasonic auxiliary water-bath lixiviate: Pericarpium Musae dry powder is added water according to 1: 6 solid-liquid ratio of weight part mix, utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate, UW lixiviate power is 80W, 70 ℃ of temperature, time 2.5h, obtains extractive substance;
D. column chromatography for separation: it is the separator column of 30mm that the above-mentioned extractive substance that obtains is placed diameter, with D918Na type resin with the flow velocity of 3.0BV/h through the resin chromatography column, the ethanol with 70% at room temperature carries out the wash-out separation and purification, obtains extracting solution;
E. extracting solution concentrates: extracting solution is concentrated under the 600mmHg negative pressure, and 75 ℃ of thickening temperatures are 65 with the extracting solution concentrating under reduced pressure to Biax, obtain liquid concentrator;
F. ethanol lixiviate: extract 2 times 75 ℃ of condition refluxed with 65%-70% ethanol, add the alcohol hydrochloric acid mixed solution in the liquid concentrator, the volume ratio of liquid concentrator and alcohol hydrochloric acid mixed solution is 1: 1;
G. crystallization: the liquid concentrator after the ethanol lixiviate is cooled to 0 ℃, and crystallization appears in container bottom, and xln is dried to permanent quality under 95 ℃ of conditions, promptly obtains serotonin hydrochloride finished product, and serotonin hydrochloride extraction yield is 87%.
Embodiment two:
A. Pericarpium Musae cleans: gather Pericarpium Musae and clean, be cut into the square shape of 1cm * 1cm;
B. drying and crushing: under 80 ℃ of conditions of temperature, dry 24h, the Pericarpium Musae of oven dry is pulverized, cross 5 mesh sieves, obtain Pericarpium Musae dry powder;
C. ultrasonic auxiliary water-bath lixiviate: Pericarpium Musae dry powder is added water according to 1: 6 solid-liquid ratio of weight part mix, utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate, UW lixiviate power is 100W, 80 ℃ of temperature, time 3.5h, obtains extractive substance;
D. column chromatography for separation: it is the separator column of 30mm that the above-mentioned extractive substance that obtains is placed diameter, with D918Na type resin with the flow velocity of 3.5BV/h through the resin chromatography column, the ethanol with 70% at room temperature carries out the wash-out separation and purification, obtains extracting solution;
E. extracting solution concentrates: extracting solution is concentrated under the 650mmHg negative pressure, and 80 ℃ of thickening temperatures are 70 with the extracting solution concentrating under reduced pressure to Biax, obtain liquid concentrator;
F. ethanol lixiviate: extract 3 times 80 ℃ of condition refluxed with 65%-70% ethanol, add the alcohol hydrochloric acid mixed solution in the liquid concentrator, the volume ratio of liquid concentrator and alcohol hydrochloric acid mixed solution is 1: 1;
G. crystallization: the liquid concentrator after the ethanol lixiviate is cooled to 0 ℃, and crystallization appears in container bottom, and xln is dried to permanent quality under 90 ℃ of conditions, promptly gets serotonin hydrochloride finished product, and serotonin hydrochloride extraction yield is 91%.
Claims (3)
1. method of utilizing Pericarpium Musae to prepare natural serotonin hydrochloride is characterized in that it being to accomplish through the following steps preparation:
A. Pericarpium Musae cleans: gather Pericarpium Musae and clean, be cut into foursquare little bulk;
B. drying and crushing: the block Pericarpium Musae oven dry 24h that under 70 ℃ of-80 ℃ of conditions of temperature, will cut; The Pericarpium Musae of oven dry is pulverized, crossed 5 mesh sieves, obtain Pericarpium Musae dry powder;
C. ultrasonic auxiliary water-bath lixiviate: Pericarpium Musae dry powder is added water according to 1: 6 solid-liquid ratio of weight part mix; Utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate; UW lixiviate power is that 80W-100W, temperature are that 70 ℃-80 ℃, time are 2-3.5h, obtains extractive substance;
D. column chromatography for separation: it is the separator column of 30mm that the above-mentioned extractive substance that obtains is placed diameter, with D918Na type resin with the flow velocity of 2.8BV/h-3.8BV/h through the resin chromatography column, the ethanol with 70% at room temperature carries out the wash-out separation and purification, obtains extracting solution;
E. extracting solution concentrates: extracting solution is concentrated under the 600-650mmHg negative pressure, and 75 ℃-80 ℃ of thickening temperatures are 60-70 with the extracting solution concentrating under reduced pressure to Biax, obtain liquid concentrator;
F. ethanol lixiviate: extract 2-4 time 75 ℃ of-80 ℃ of condition refluxed with 65%-70% ethanol, add the alcohol hydrochloric acid mixed solution in the liquid concentrator, the volume ratio of liquid concentrator and alcohol hydrochloric acid mixed solution is 1: 1;
G. crystallization: the liquid concentrator after the ethanol lixiviate is cooled to 0 ℃, and crystallization appears in container bottom, and xln is dried to permanent quality under 90 ℃ of-100 ℃ of conditions, promptly obtains serotonin hydrochloride finished product.
2. a kind of method of utilizing Pericarpium Musae to prepare natural serotonin hydrochloride according to claim 1 is characterized in that:
A. gather Pericarpium Musae and clean, be cut into 1.5cm * 1.5cm square shape;
B. under 75 ℃ of conditions of temperature, dry 24h;
C. utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate, UW lixiviate power is 80W, 70 ℃ of temperature, time 2.5h;
D. with D918Na type resin with the flow velocity of 3.0BV/h through the resin chromatography column;
E. extracting solution is concentrated under the 600mmHg negative pressure, 75 ℃ of thickening temperatures are 65 with the extracting solution concentrating under reduced pressure to Biax;
F. extract 2 times 75 ℃ of condition refluxed with 65%-70% ethanol;
G. xln is dried to permanent quality under 95 ℃ of conditions, promptly obtains serotonin hydrochloride finished product, the serotonin hydrochloride extraction yield that obtains is 87%.
3. a kind of method of utilizing Pericarpium Musae to prepare natural serotonin hydrochloride according to claim 1 is characterized in that:
A. gather Pericarpium Musae and clean, be cut into the square shape of 1cm * 1cm;
B. under 80 ℃ of conditions of temperature, dry 24h;
C. utilize KH-100SP type double frequency numerical control supersonic cleanser water-bath lixiviate, UW lixiviate power is 100W, 80 ℃ of temperature, time 3.5h;
D. with D918Na type resin with the flow velocity of 3.5BV/h through the resin chromatography column;
E. extracting solution is concentrated under the 650mmHg negative pressure, 80 ℃ of thickening temperatures are 70 with the extracting solution concentrating under reduced pressure to Biax;
F. extract 3 times 80 ℃ of condition refluxed with 65%-70% ethanol;
G. xln is dried to permanent quality under 90 ℃ of conditions, promptly gets serotonin hydrochloride finished product, the serotonin hydrochloride extraction yield that obtains is 91%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108219913A (en) * | 2018-02-27 | 2018-06-29 | 浙江绿保再生资源科技有限公司 | A kind of composite flocculation agent and waste lubricating oil flocculating regeneration technique |
| CN108452107A (en) * | 2017-02-10 | 2018-08-28 | 百岳特生物科技（上海）有限公司 | Application of banana peel extract in regulation and control of TPH1, DDC and/or AANAT gene expression |
| CN109329584A (en) * | 2018-11-08 | 2019-02-15 | 浙江海洋大学 | Feed for symphysodon aequifasciata |
| CN110483362A (en) * | 2019-09-11 | 2019-11-22 | 广东石油化工学院 | The process of serotonin is extracted in a kind of response phase method optimization from banana |
| CN112358433A (en) * | 2020-11-20 | 2021-02-12 | 保定保利瑞合生物科技有限公司 | Method for preparing 5-hydroxytryptamine hydrochloride by using enzyme-catalyzed fermentation broth containing 5-hydroxytryptamine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101337921A (en) * | 2008-08-08 | 2009-01-07 | 青海清华博众生物技术有限公司 | Method for preparing 5-hydroxytryptamine from hippophae rhamnoides |
| WO2011018700A1 (en) * | 2009-08-12 | 2011-02-17 | Munisekhar Medasani | Natural extract from whole banana fruit (musa spp.) |
-
2012
- 2012-04-11 CN CN2012101055820A patent/CN102633707A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101337921A (en) * | 2008-08-08 | 2009-01-07 | 青海清华博众生物技术有限公司 | Method for preparing 5-hydroxytryptamine from hippophae rhamnoides |
| WO2011018700A1 (en) * | 2009-08-12 | 2011-02-17 | Munisekhar Medasani | Natural extract from whole banana fruit (musa spp.) |
Non-Patent Citations (2)
| Title |
|---|
| G.VETTORAZZI: "5-hydroxytryptamine content of bananas and banana products", 《FOOD AND COSMETICS TOXICOLOGY》 * |
| 雷国强等: "香蕉中5-羟色胺的荧光测定和薄层鉴定", 《中药材》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108452107A (en) * | 2017-02-10 | 2018-08-28 | 百岳特生物科技（上海）有限公司 | Application of banana peel extract in regulation and control of TPH1, DDC and/or AANAT gene expression |
| CN108219913A (en) * | 2018-02-27 | 2018-06-29 | 浙江绿保再生资源科技有限公司 | A kind of composite flocculation agent and waste lubricating oil flocculating regeneration technique |
| CN109329584A (en) * | 2018-11-08 | 2019-02-15 | 浙江海洋大学 | Feed for symphysodon aequifasciata |
| CN110483362A (en) * | 2019-09-11 | 2019-11-22 | 广东石油化工学院 | The process of serotonin is extracted in a kind of response phase method optimization from banana |
| CN112358433A (en) * | 2020-11-20 | 2021-02-12 | 保定保利瑞合生物科技有限公司 | Method for preparing 5-hydroxytryptamine hydrochloride by using enzyme-catalyzed fermentation broth containing 5-hydroxytryptamine |
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Application publication date: 20120815 |