CN102633368A - Light quality and illumination intensity deployment strategy method by utilizing microalga-purified biogas slurry - Google Patents
Light quality and illumination intensity deployment strategy method by utilizing microalga-purified biogas slurry Download PDFInfo
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- CN102633368A CN102633368A CN2012101003510A CN201210100351A CN102633368A CN 102633368 A CN102633368 A CN 102633368A CN 2012101003510 A CN2012101003510 A CN 2012101003510A CN 201210100351 A CN201210100351 A CN 201210100351A CN 102633368 A CN102633368 A CN 102633368A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
The invention belongs to the technical field of environmental engineering and particularly relates to a light quality and illumination intensity deployment strategy method by utilizing microalga-purified biogas slurry. The method disclosed by the invention comprises the following steps of: during the process of culturing the microalga-purified biogas slurry by utilizing biogas slurry and an LED (light-emitting diode) lamp with specific light quality as a nutritional matrix and an artificial light source, adopting a multistep type illumination intensity regulation strategy, namely regulating the illumination intensity of the LED lamp during the different growth stages of microalga to realize the purposes of saving energy and promoting the growth of the microalga to promote a biogas slurry purification effect. In comparison with a constant type illumination strategy, the multistep type illumination regulation strategy disclosed by the invention can save light energy during the adaptive phase and logarithmic phase of microalga culture. The method disclosed by the invention is good in practicality, and simple and easy to operate; during the process of utilizing the microalga-purified biogas slurry, the light energy can be greatly saved and the economy of a biogas slurry purification method can be improved; and furthermore, the influence caused by light inhibition or insufficient illumination can be avoided, thus the growth of the microalga is further promoted, and the biogas slurry purification effect is improved.
Description
Technical field
The invention belongs to field of environment engineering technology, be specifically related to a kind of light quality and tactful novel method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid.
Background technology
China's biogas cause started from before and after nineteen thirty, the equal unregulated power supply in most at that time cities and towns, and biogas fermentation is mainly used in the illumination of some shops, monastery etc., and the seventies in last century, China started the upsurge that once develops biogas again.In recent years, because problems such as the international and domestic energy, environment is increasingly serious, the biogas fermentation technology more and more is much accounted of.The Ministry of Agriculture has promoted the development of China's biogas cause greatly " ecology homestead rich people plan " that start and implemented to attach most importance to, meet with biogas comprehensive utilization and construction current agricultural and rural economy developmental stage in January, 2000.
But in biogas career development process, natural pond liquid is difficult to effectively handled because of its pollutant load is high for a long time, and the potential threat of its environmental pollution has restricted the development of biogas cause in China greatly.The organotrophy constituent content is abundant in the liquid of natural pond.The process of biogas fermentation is the hydrocarbon metabolism that waits element, and hydrocarbon in the fermentation substrate waited warp to ferment and be converted into biogas, and nitrogen wherein, phosphorus, potassium etc. then remain in the fermentation residue, and owing to structure is comparatively simple, generally can directly be absorbed by crop.If these nutritive elements are discharged in the natural water, then very likely cause the eutrophy of water body, even the danger that produces algal tufa is arranged.Natural pond liquid contains abundant mineral element, and it is present in originally participates in microbial metabolism in the fermentation raw material, residues in again at last in the liquid of biogas fermentation residue natural pond, and these abundant mineral element great majority all are that crop growth is necessary.Also also have great deal of bioactive substances and each seed amino acid in the liquid of natural pond.
Existing natural pond liquid processing method all is to use existing municipal sewage plant and the treatment facility of handling other trade effluents basically.This method is not only because treatment facility has the specific aim poor processing effect, and economy is also relatively poor, is difficult to obtain actual large-scale application.Also have some with natural pond liquid as a kind of biomass resource method of recycling in addition, as: natural pond liquid is carried out blade face liquid fertilizer and organic as agricultural fertilizer topdresses etc.; Natural pond liquid as biological pesticide, is used in seed soaking, prevention and elimination of disease and pests etc.; With natural pond liquid as feed, in order to breed fish, to raise pigs and earthworm etc.; With natural pond liquid as cultivating feed liquid, in order to culturing edible fungus, soilless culture, cultivation seedling etc.These resource utilization method all are small-scale, and actual effect is difficult to assessment, the particularly security of food.And they are in use because relate to the step that natural pond liquid is directly used, so be difficult to guarantee that it can not be lost in the environment water pollutes.
Little algae is with its light and functioning efficiency and high growth velocity and can absorb nutritive element in the surrounding environment in a large number and make its spontaneous continuous propagation efficiently.The growth of little algae in water body environment generally can be divided into standby period, logarithmic phase, stationary phase and decline phase.The adaptive phase of growth itself because the less and required intensity of illumination of density is less, might cause light to suppress the growth of little algae if adopt unalterable intensity of illumination this moment; And its logarithmic phase density in growth constantly increases; Density reaches maximum after reaching stationary phase; This moment, microalgae cell influenced lighting effect because cover each other; So this stage needs bigger intensity of illumination, might influence the growth of little algae if still adopt unalterable care intensity this moment because illumination is not enough.Simultaneously; The staged that the difference that requires for intensity of illumination at different growth phases according to little algae is taked is supplied with the strategy of illumination; Can save the luminous energy consumption of cultivating adaptive phase and logarithmic phase in little algae greatly, improve the economic benefit of utilizing little algae to purify natural pond liquid technology.
Summary of the invention
The object of the present invention is to provide a kind of light quality and tactful novel method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid; It adopts stepped illumination strategy; The growth that not only can promote little algae is to promote the decontamination effect improving of natural pond liquid; Can also save a large amount of luminous energy, improve the economic and practical of this technological method.Its concrete steps are following:
(1) the LED lamp source of different light medium is set, according to strength condition upgrowth situation and the natural pond liquid decontamination effect improving of the little algae of test down, selects the light quality condition of the best in identical culture condition and constant light;
(2) the intensity of illumination experiment group that increases gradually is set, tests the upgrowth situation and the natural pond liquid decontamination effect improving of little algae each growth period under each intensity of illumination, determine the optimum intensity of illumination of little algae in each different growing stage;
(3) select of intensity of illumination minimum in the intensity of illumination group of the good and natural pond liquid good purification of upgrowth situation in each growth period of little algae, make final staged illumination strategy according to the time series of micro algae growth.
Among the present invention, be according to the adaptive phase for the division of the growth period of little algae in the process of confirming staged illumination strategy, logarithmic phase and stationary phase divide.Specifically, when adopting staged illumination strategy, little algae carries out small intensity illumination in the adaptive phase of growth because algae cell density is little, suppresses to avoid light; Logarithmic phase in growth begins to occur the phenomenon that frustule covers each other because alga cells density increases fast, then must accept the illumination of relative higher-strength, and is not enough to avoid illumination; Because reaching peak, alga cells density have very serious mutual masking phenomenon, this moment then must accept the illumination of maximum intensity in the stationary phase of growth, and not enough to avoid illumination.
Among the present invention, for set intensity of illumination experimental group in the process of confirming staged illumination strategy, with known light suppress with the not enough intensity of illumination as the upper and lower bound intensity of illumination, thin as far as possible then divided light photograph strength increase amount.
Among the present invention, compare, promote the degree of natural pond liquid decontamination effect improving with degree of power conservation and the promotion micro algae growth of confirming staged illumination strategy with selected at last staged illumination strategy with according to the definite constant formula illumination strategy of maximum intensity of illumination.
The invention has the advantages that:
(1) light source that is adopted is the LED lamp, has the narrower spectrum band of common relatively fluorescent lamp, can produce the light quality of specific wavelength, cultivates for little algae to have the effect of efficient energy-saving;
(2) the staged illumination strategy that is adopted is to divide according to the growth cycle of little algae; Promptly avoided too high and light that produce suppresses, avoided intensity of illumination in stationary phase not enough and influenced the growth and the purification of natural pond liquid of little algae again in the intensity of illumination of adaptive phase;
(3) the staged illumination strategy that is adopted is divided according to the growth cycle of little algae; Adopt less intensity of illumination in the early stage of micro algae growth; Later stage in growth is adopted bigger intensity of illumination, can practice thrift luminous energy like this, improves the economic and practical of this technological method.
Description of drawings
The dry weight of chlorella changes under Fig. 1 different light medium condition.
Chlorella is for the variation of the COD clearance of natural pond liquid under Fig. 2 different light medium condition.
Chlorella is for the variation of the TN clearance of natural pond liquid under Fig. 3 different light medium condition.
Chlorella is for the variation of the TP clearance of natural pond liquid under Fig. 4 different light medium condition.
The dry weight of chlorella changes under Fig. 5 different light intensity degree condition.
Chlorella is for the variation of the COD clearance of natural pond liquid under Fig. 6 different light intensity degree condition.
Chlorella is for the variation of the TN clearance of natural pond liquid under Fig. 7 different light intensity degree condition.
Chlorella is for the variation of the TP clearance of natural pond liquid under Fig. 8 different light intensity degree condition.
Fig. 9 optimal strategy energy-saving effect.
Embodiment
Through embodiment the present invention is carried out detailed explanation below.
Embodiment
A kind of light quality and tactful novel method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid, it utilizes the LED lamp of natural pond liquid and specific light quality to come culturing microalgae to purify natural pond liquid as nutraceutical matrix and source of artificial light respectively.In this process, regulate strategy through the staged intensity of illumination, both regulated the intensity of illumination of LED lamp at the different growth phases of little algae, realize energy-conservation with promote micro algae growth to promote the purpose of natural pond liquid decontamination effect improving.
Below be concrete step:
(1) be provided with chlorella (
Chlorella Vulgaris) under the LED lamp source irradiation of white, red, blue, green, Huang and purple light quality, be that 12h:12h is a condition with 25 degrees centigrade of culture temperature, pH=6.5, Light To Dark Ratio, the 2000Lux intensity of illumination.
The result shows for chlorella that for the multiplication rate of any growth phase its LED light quality ordering all be red>white>yellow>purple>blueness>green (Fig. 1); For the clean dirty ability of any growth phase, its LED light quality ordering all be red for chlorella>yellow>white>blueness>purple>green (Fig. 2-4).So take all factors into consideration, draw the ordering of LED light quality for red>white>yellow>blueness>purple>green, decision adopts the LED red light as source of artificial light.
(2) with reference to result shown in Figure 5, the growth of chlorella can be divided into adaptive phase, logarithmic phase, stationary phase and decline phase four-stage, both 0-48h-96h-144h-240h four-stage.The intensity of illumination experimental group 800Lux, 1200Lux, 1600Lux and the 2000Lux that increase gradually are set, the upgrowth situation and the natural pond liquid decontamination effect improving of test chlorella each growth period under each intensity of illumination.
For chlorella in the intensity of illumination of any growth phase for the influence of clean dirty ability; The optimal strategy of its LED light intensity is 0-48h-96h-144h three phases, and each stage is adopted intensity of illumination 800Lux, 1200Lux and 1600Lux (Fig. 6-8) successively.
(3) the staged illumination strategy that will select at last and compare according to the definite constant formula illumination strategy of maximum intensity of illumination (2000Lux), with the degree of power conservation of confirming staged illumination strategy with promote micro algae growth to promote the degree of natural pond liquid decontamination effect improving.
The result of comprehensive above (1) and (2); Drawing optimal strategy is to adopt the LED red light; With intensity of illumination then is to be divided into 0-48h-96h-144h three phases; Each stage is adopted intensity of illumination 800Lux, 1200Lux and 1600Lux successively, and this optimum illumination strategy is than energy-conservation about 40% (Fig. 9) of constant intensity of illumination (2000Lux) method.End-result shows that chlorella dry weight density climax can reach 316.67 mg/L, and the clearance of natural pond liquid COD, TN and TP can reach 92.34%, 80.24% and 89.50% (Fig. 5-8) respectively.
Claims (4)
1. a light quality and an intensity of illumination of utilizing little algae to purify natural pond liquid allocated tactful method, it is characterized in that concrete steps are:
(1) the LED lamp source of different light medium is set, according to strength condition upgrowth situation and the natural pond liquid decontamination effect improving of the little algae of test down, selects the light quality condition of the best in identical culture condition and constant light;
(2) the intensity of illumination experiment group that increases gradually is set, tests the upgrowth situation and the natural pond liquid decontamination effect improving of little algae each growth period under each intensity of illumination, determine the optimum intensity of illumination of little algae in each different growing stage;
(3) select of intensity of illumination minimum in the intensity of illumination group of the good and natural pond liquid good purification of upgrowth situation in each growth period of little algae, make final staged illumination strategy according to the time series of micro algae growth.
2. light quality and the tactful method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid according to claim 1; It is characterized in that; Division for the growth period of little algae in the process of confirming staged illumination strategy is according to the adaptive phase, and logarithmic phase and stationary phase divide.
3. light quality and the tactful method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid according to claim 2; It is characterized in that; For set intensity of illumination experimental group in the process of confirming staged illumination strategy; With known light suppress with the not enough intensity of illumination as the upper and lower bound intensity of illumination, thin as far as possible then divided light photograph strength increase amount.
4. light quality and the tactful method of intensity of illumination allotment of utilizing little algae to purify natural pond liquid according to claim 1; It is characterized in that; Compare with selected at last staged illumination strategy with according to the definite constant formula illumination strategy of maximum intensity of illumination, promote the degree of natural pond liquid decontamination effect improving with degree of power conservation and the promotion micro algae growth of confirming staged illumination strategy.
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| CN109644860A (en) * | 2019-01-17 | 2019-04-19 | 深圳市千百辉照明工程有限公司 | Inhibit the new light sources of algal grown in a kind of illumination of solution cavity |
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Cited By (2)
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| CN105087430A (en) * | 2015-07-27 | 2015-11-25 | 厦门市科环海洋生物科技有限公司 | Culture medium and culture method of high-concentration photosynthetic bacteria |
| CN109644860A (en) * | 2019-01-17 | 2019-04-19 | 深圳市千百辉照明工程有限公司 | Inhibit the new light sources of algal grown in a kind of illumination of solution cavity |
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Application publication date: 20120815 |