CN102631261A - Wet composite dressing and sealed negative pressure drainage device therefrom - Google Patents
Wet composite dressing and sealed negative pressure drainage device therefrom Download PDFInfo
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- CN102631261A CN102631261A CN2012101380935A CN201210138093A CN102631261A CN 102631261 A CN102631261 A CN 102631261A CN 2012101380935 A CN2012101380935 A CN 2012101380935A CN 201210138093 A CN201210138093 A CN 201210138093A CN 102631261 A CN102631261 A CN 102631261A
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Abstract
The invention discloses a wet composite dressing and a sealed negative pressure drainage device therefrom, which relate to a medical product. The sealed negative pressure drainage device comprises a wet bacterial cellulose-chitosan fiber composite dressing, an adhesive semipermeable membrane, a negative pressure plate and a drainage tube connected with a negative pressure device. The negative pressure drainage device disclosed by the invention has the advantages of low cost and simpleness and easiness in preparation process and is convenient to operate. The whole device can be cut and combined at will according to the shape and the position of a wound surface, has multiple functions of antibiosis, moisture retention and negative-pressure drainage, can be used for curing chronic and unhealed wound, acute intractable wounds, burns and large-area soft tissue defects and/ or infections.
Description
Technical field
The present invention relates to the bio-medical technical field, particularly a kind of moist combine dressing and with the closed negative pressure drainage device of its preparation.Specifically with method of electrostatic spinning obtain a kind of have preserve moisture, the Bacterial cellulose recombination chitosan fiber moist dressing of guide functions, and be combined into a kind of closed negative pressure drainage device with semipermeable membrane, negative pressure dish and the drainage tube that is connected negtive-pressure implement.
Background technology
The closed negative pressure drainage technology of wound surface (vacuum sealing drainage VSD) is a kind of Wound treating new technique that grows up this year.Its utilizes can paste semipermeable membrane and makes the open wound sealing, uses negtive-pressure implement to produce certain negative pressure, acts on the wound surface after the debridement through porous dressing, absorbs the wound transudate and flows out through drainage tube.People such as doctor Fleischmann combined traditional negative pressure drainage and present impermeable plastic wound dressing in 1992, were applied to treat the open fracture wound surface and achieved success.Argenta in 1997 and Morykwas etc. discover that VSD can promote the wound surface local blood circulation, promote granulation tissue production, reduce tissue bacterial content, improve the survival rate of flap or free skin-grafting.Along with VSD The Application of Technology and development, increasing research and clinical report have confirmed that the VSD technology comprises acute soft tissue damaged, all kinds of chronic (pressure, vascular, diabetic) ulcer at all kinds of acute and chronic wounds of treatment, surgical incision splits or infection etc. has curative effect preferably.This therapy can be quickened the blood circulation of wound site, significantly promotes new vessels to get into wound surface, stimulates the growth of granulation tissue; Fully drain alleviates edema, reduces and pollutes; Bacteria growing inhibiting can directly be accelerated wound healing, or creates conditions for surgical repair.The VSD technology has obtained rapid popularization in developed countries such as America and Europes at present, is described as the revolutionary technology of current treatment wound, is the new milestone in Wound care field.And each medical fields such as wound, burn, shaping have been widely used in.States such as Germany in 2003, Austria include this technology in wound treatment guide.Over past ten years, China begins to introduce this technology, and is accumulating a large amount of experiences aspect basic research and the clinical practice.
Closed negative pressure drainage technology is that traditional negative pressure drainage method is combined with closure dressing; This technology adopts the foam porous dressing that has drainage tube to cover or fill wound surface; The reuse semipermeable membrane seals dressing; Make whole system become confined space, at last drainage tube is connected on the negtive-pressure implement, promote wound healing through the control negative pressure.Now VSD technology basic configuration comprises negative pressure source (comprise medical attractor, negative pressure draw bottle), and drainage tube, can be pasted sealing with semipermeable membrane, union joint, drainage container etc. at foam porous dressing.Wherein foam porous dressing plays key effect as the intermediary between negative-pressure drainage tube and wound surface in whole closed negative pressure drainage device.It requires dressing inside to have the porous network that is interconnected, to guarantee that organize transudate and metabolite that vacuum suction goes out can be by normal sucking-offs; Dressing simultaneously and the direct contact need of wound have excellent biological compatibility, can promote wound healing.Dressing materials commonly used at present is a polyvinyl alcohol, and the polyvinyl alcohol cost is higher, makes the loose structure complex process, main dependence on import.Cause at this type of Therapeutic Method cost of China very highly, increased heavy financial burden to the patient.In addition, the closed negative pressure drainage device of present clinical employing is generally medical institutions and adopts the accessory self-control of different vendor to form, and makes whole system unstable, and the safety in the illegal assurance use.And mostly the closed negative pressure drainage device of import manufacturers produce is fixed specifications and models, and the needs and the price that are difficult to satisfy different wounds position are very expensive.Therefore, the substitute products of developing a kind of low cost and making things convenient for combination to use are task of top priority.
Summary of the invention
The purpose of this invention is to provide a kind of moist combine dressing and with the closed negative pressure drainage device of its preparation.Relate to a kind of medical article.The present invention prepares that process is simple, easy to operate, cost is low; Whole device can cut randomly according to the shape of wound surface, make up, and is easy to use.
A kind of moist combine dressing adopts method of electrostatic spinning, and chitosan is compounded in the Bacterial cellulose surface, obtains Bacterial cellulose recombination chitosan fiber moist dressing.Bacterial cellulose is as a kind of good biomaterial, has its unique physics, chemical property: BC has natural three-dimensional manometer network structure; High-tensile and elastic modelling quantity; High-hydrophilic, high ventilation, suction, water permeability, outstanding retentiveness and high wet strength.In addition, big quantity research shows that Bacterial cellulose has in the good body, the external biological compatibility and good biodegradability, and this makes Bacterial cellulose itself can be applied to the bio-medical field.The simple Bacterial cellulose of external employing is reported as dressing is existing, and industrialization is used for clinical.Based on above characteristics, the present invention utilizes the water absorbing properties of Bacterial cellulose itself with the matrix material of Bacterial cellulose as closed negative pressure drainage dressing, can be at the absorption wound fluid and the metabolite that guarantee continuous and effective on the basis of biological safety.In addition, in the market negative pressure drainage dressing is dry state dressing basically.
In order to improve the drain performance of Bacterial cellulose, this patent is compounded in chitin fiber on the Bacterial cellulose.The chitosan material hydrophilicity is relatively poor, and with after Bacterial cellulose contacts, chitin fiber has destroyed the surface tension of Bacterial cellulose itself, is adding under the condition of negative pressure, makes transudate flow out the purpose that reach negative pressure drainage through chitin fiber smoothly.Simultaneously, chitosan has certain anti-microbial property, and excellent biological compatibility has been proved to be and can be applied to the wound dressing product safely and effectively.Adopt method of electrostatic spinning with Bacterial cellulose and the compound Bacterial cellulose recombination chitosan fiber moist dressing that obtains of chitin fiber, that this dressing has is antibiotic, preserve moisture and the negative pressure drainage multi-efficiency.Can be applied to cure the chronic sexual trauma of not healing, press acute intractable wound, burn and large tracts of land soft tissue defects and/or infection.
Moist combine dressing of the present invention; Concrete method for preparing is: adopt method of electrostatic spinning; It is to be mixed with the chitosan-acetic acid solution that mass fraction is 0.5-5% in the 0.1-5% acetum that chitosan is dissolved in mass fraction, and the chitosan-acetic acid solution of formation evenly covers the Bacterial cellulose surface through spinneret orifice.Chitosan wherein is preferably molecular weight 5-30 ten thousand.The chitosan fibre diameter is 200-700nm in the Bacterial cellulose recombination chitosan fiber moist dressing that obtains, and the moisture mass fraction of combine dressing is 30-90%, and thickness is the 4.0-10.0 millimeter.
According to a kind of embodiment preferred; Moist combine dressing of the present invention, method for preparing is: adopting method of electrostatic spinning, is that the Bacterial cellulose of 50-90% covers the surge drum top with moisture mass fraction; It is 5-40kV that electrostatic spinning voltage is set; Syringe pump speed is 0.1-2ml/h, and the spray webbing syringe needle is 5-20cm apart from the Bacterial cellulose distance, and chitosan-acetic acid solution is evenly covered the Bacterial cellulose surface through spinneret orifice.
Above-mentioned Bacterial cellulose is by one or more outputs in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter.Known and the extensive use of its production method.
As optimized technical scheme:
The method for preparing of aforesaid bacteria cellulose material, its seed culture medium are 3～10w/v% glucose, 0.01～0.1 w/v% calcium carbonate, the water of 0.01～0.1 w/v% yeast extract and surplus.Fermentation medium is 3～10w/v% glucose, 0.1～0.4 w/v% sodium hydrogen phosphate, 0.1～0.4 w/v% potassium dihydrogen phosphate, 0.1～0.5 w/v% yeast extract, the water of 0.1～0.5 w/v% peptone and surplus.
Fermentation strain is an acetobacter xylinum; Remove tropina and stick to the residual media on the cellulose membrane through separating to purify after fermentation is accomplished.As: can Bacterial cellulose be immersed in the quality percentage composition is in 1～8% the NaOH aqueous solution, under 60～100 ℃ temperature, heats 3～6h.The reuse redistilled water washes to neutrality repeatedly.
Further, the present invention provides a kind of closed negative pressure drainage device, comprises described moist combine dressing, can paste semipermeable membrane, negative pressure dish and connect the drainage tube of negtive-pressure implement.When it uses, the Bacterial cellulose combine dressing is covered or is filled in wound, the negative pressure dish is placed in the centre position above dressing, can paste semipermeable membrane and pass the negative pressure dish and cover the dressing top, forms closed system.At last drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement (negative pressure process units).
Closed negative pressure drainage device of the present invention, wherein said moist combine dressing are preferably and handle the moisture mass fraction that obtains through setting-out is the Bacterial cellulose recombination chitosan fiber moist dressing of 30-50%.
Closed negative pressure drainage device of the present invention, the wherein said semipermeable membrane of pasting is to have the transparent membrane of folk prescription to airing function.Be mixed and made into by one or more of polyurethane, polyacrylic acid or polyester.Its role is to seal Bacterial cellulose recombination chitosan fiber moist dressing and negative pressure dish, make whole device form closed environment, so that produce negative pressure.
Closed negative pressure drainage device of the present invention, wherein said negative pressure dish is made up of hollow cone body medical silicone tube and disc porous medical resin; Wherein the bottom surface of hollow cone body is a concentric discs; Inlay circular porous medical resin in the concentric discs; One tubulose union joint is arranged at the cone top, union joint perpendicular to cone radially and be parallel to bottom surface, there is the inverted cone draw-in groove at the union joint place.
Optimized technical scheme is: the bottom surface of hollow cone body is the concentric discs of internal diameter 10mm external diameter 26mm, inlays circular porous medical resin in the concentric discs; One tubulose union joint is arranged at the cone top, union joint perpendicular to cone radially and be parallel to bottom surface, there is the inverted cone draw-in groove at the union joint place, makes things convenient for drainage tube to insert and prevents that drainage tube from coming off in use.The negative pressure dish is the device that connects dressing, transmits negative pressure and wound fluid derivation dressing.In use, the quantity of negative pressure dish and position can be confirmed according to shape, the position of concrete wound, to guarantee having uniform negative pressure, especially dressing and wound contact surface to keep lasting, uniform negative pressure in the whole negative pressure drainage device.In addition, the circular porous medical resin of inlaying in the concentric discs can guarantee the negative pressure uniform distribution, prevents owing to Wound exudate, dressing precipitate too much stop up drainage tube.
Closed negative pressure drainage device of the present invention, wherein said drainage tube are hollow pipe; Its material is transparent medical silica-gel, politef, polyethylene or polrvinyl chloride.During use, drainage tube one end connects the union joint of negative pressure dish, and an end connects negtive-pressure implement.
Closed negative pressure drainage device of the present invention, the negtive-pressure implement that described drainage tube connected, the negative pressure that can produce should be-10 to-25kpa, and the dressing that negative pressure drainage device is inner and the negative pressure of wound surface contact surface are 10-20kpa.The generation of negative pressure can reduce wound surface surface oxygen dividing potential drop, thereby a lot of antibacterials multiplication rate under the situation of hypoxia is reduced, and simultaneously, the partial vacuum effect that negative pressure produces is distorted bacteria cell wall and destroys, and causes the death of antibacterial.Negative pressure drainage can promote the debridement and the healing of different wounds, and negative pressure is too small can not to guarantee fully drawing of wound surface exudate, and negative pressure is too high can to cause that again the local blood perfusion is bad; The research report is for fat and hypodermic wound surface; Optimum pressure is-10kPa is to-11kPa, and for wound surface the best of muscle is-13kPa, generally negative pressure value is set at clinically-10kPa extremely-20kPa; Can keep wound surface pressure like this, the unlikely again damage that causes local organization.Use suitable negative pressure and can promote drain, accelerate blood flow, and the size of different tissues application of negative pressure is not quite similar also.Therefore should regulate negative pressure value according to the practical situation of different tissues and negative pressure drainage device in actual use.Preferred negtive-pressure implement is to produce the negtive-pressure implement that continues with the negative pressure at intermittence.
Closed negative pressure drainage device of the present invention; During use Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; Can paste semipermeable membrane and pass the negative pressure dish and cover the dressing top, semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement (negative pressure process units).The length of the quantity of the size shape of Bacterial cellulose recombination chitosan fiber moist dressing, the size shape that can paste semipermeable membrane, negative pressure dish and placement location, drainage tube all can cut randomly, make up according to shape, the position of actual wound surface in the whole closed negative pressure drainage device.
Compared with prior art, the invention has the beneficial effects as follows:
(1) Bacterial cellulose that adopts microorganism culturing to make substitutes foam porous material as negative pressure drainage dressing matrix, has significantly reduced production cost.The preparation process is simple and easy to, and does not contain any toxic solvent, can not bring problems such as environmental pollution and ecological crisis, is fit to produce in enormous quantities, meets the instructions for use of biomedical sector.
(2) use method of electrostatic spinning and prepare Bacterial cellulose recombination chitosan fiber moist dressing, make closed negative pressure drainage device have antibiotic, preserve moisture and the negative pressure drainage multi-efficiency.
(3) closed negative pressure drainage device anabolic process is simple, easy to operate and can cut randomly, make up according to shape, the position of wound surface.Can be applied to cure the chronic sexual trauma of not healing, press acute intractable wound, burn and large tracts of land soft tissue defects and/or infection.
Description of drawings
Fig. 1 is the structural representation of closed negative pressure drainage device of the present invention;
Wherein, 1-Bacterial cellulose recombination chitosan fiber moist dressing; 2-can paste semipermeable membrane; 3-negative pressure dish; The 4-drainage tube.
The specific embodiment
Below in conjunction with the specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1:
(1) Bacterial cellulose is prepared by acetobacter xylinum, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 10w/v% glucose, 0.01 w/v% calcium carbonate, the water of 0.01 w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 3w/v% glucose, 0.1 w/v% sodium hydrogen phosphate, 0.1 w/v% potassium dihydrogen phosphate, 0.1 w/v% yeast extract, the water of 0.1 w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 1% the NaOH aqueous solution, and under 60 ℃ temperature, heat 6h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 0.1% acetum be 5% chitosan-acetic acid solution for 50,000 chitosan with molecular weight.With moisture mass fraction is that 50% Bacterial cellulose covers the surge drum top; It is 40kV that electrostatic spinning voltage is set; Syringe pump speed is 2ml/h; The spray webbing syringe needle is 5cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 200nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 50% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using; As shown in Figure 1; Bacterial cellulose recombination chitosan fiber moist dressing 1 is covered or is filled in wound surface, and negative pressure dish 3 is placed in the centre position above dressing 1, and the semipermeable membrane pasted 2 of polyurethane preparation is passed negative pressure dish 3 and covers dressing 1 top; Semipermeable membrane 2 contacts with the bottom surface concentric discs of negative pressure dish 3, forms closed system.At last medical silica-gel drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 2:
(1) Bacterial cellulose is prepared by rhizobium, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 3w/v% glucose, 0.1 w/v% calcium carbonate, the water of 0.1 w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 10w/v% glucose, 0.4 w/v% sodium hydrogen phosphate, 0.4 w/v% potassium dihydrogen phosphate, 0.5 w/v% yeast extract, the water of 0.5 w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 2% the NaOH aqueous solution, and under 70 ℃ temperature, heat 5h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 0.5% acetum be 4% chitosan-acetic acid solution for 100,000 chitosan with molecular weight.With moisture mass fraction is that 60% Bacterial cellulose covers the surge drum top; It is 30kV that electrostatic spinning voltage is set; Syringe pump speed is 1.5ml/h; The spray webbing syringe needle is 10cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 400nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 40% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyacrylic acid preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last politef drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 3:
(1) Bacterial cellulose is prepared by Sarcina, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 5w/v% glucose, 0.05w/v% calcium carbonate, the water of 0.02w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 5w/v% glucose, 0.2w/v% sodium hydrogen phosphate, 0.2w/v% potassium dihydrogen phosphate, 0.2w/v% yeast extract, the water of 0.2w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 3% the NaOH aqueous solution, and under 80 ℃ temperature, heat 4h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 1% acetum be 3% chitosan-acetic acid solution for 150,000 chitosan with molecular weight.With moisture mass fraction is that 70% Bacterial cellulose covers the surge drum top; It is 25kV that electrostatic spinning voltage is set; Syringe pump speed is 1ml/h; The spray webbing syringe needle is 15cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 500nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 30% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyester manufacture is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last polyethylene drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 4:
(1) Bacterial cellulose is prepared by Rhodopseudomonas, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 7w/v% glucose, 0.07w/v% calcium carbonate, the water of 0.05w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 7w/v% glucose, 0.3w/v% sodium hydrogen phosphate, 0.3w/v% potassium dihydrogen phosphate, 0.3w/v% yeast extract, the water of 0.3w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 4% the NaOH aqueous solution, and under 90 ℃ temperature, heat 3h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 2% acetum be 2% chitosan-acetic acid solution for 200,000 chitosan with molecular weight.With moisture mass fraction is that 80% Bacterial cellulose covers the surge drum top; It is 20kV that electrostatic spinning voltage is set; Syringe pump speed is 0.8ml/h; The spray webbing syringe needle is 20cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 600nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 50% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyurethane and polyacrylic acid blend preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last polrvinyl chloride drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 5:
(1) Bacterial cellulose is prepared by achromobacter, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 9w/v% glucose, 0.02w/v% calcium carbonate, the water of 0.07w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 8w/v% glucose, 0.3w/v% sodium hydrogen phosphate, 0.2w/v% potassium dihydrogen phosphate, 0.2w/v% yeast extract, the water of 0.5w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 5% the NaOH aqueous solution, and under 100 ℃ temperature, heat 3h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 3% acetum be 1% chitosan-acetic acid solution for 250,000 chitosan with molecular weight.With moisture mass fraction is that 90% Bacterial cellulose covers the surge drum top; It is 15kV that electrostatic spinning voltage is set; Syringe pump speed is 0.6ml/h; The spray webbing syringe needle is 20cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 650nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 40% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyurethane and polyester blend preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last polrvinyl chloride drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 6:
(1) Bacterial cellulose is prepared by Alcaligenes, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 10w/v% glucose, 0.03w/v% calcium carbonate, the water of 0.03w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 10w/v% glucose, 0.4w/v% sodium hydrogen phosphate, 0.1w/v% potassium dihydrogen phosphate, 0.5w/v% yeast extract, the water of 0.5w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 6% the NaOH aqueous solution, and under 100 ℃ temperature, heat 4h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 4% acetum be 0.5% chitosan-acetic acid solution for 300,000 chitosan with molecular weight.With moisture mass fraction is that 80% Bacterial cellulose covers the surge drum top; It is 10kV that electrostatic spinning voltage is set; Syringe pump speed is 0.4ml/h; The spray webbing syringe needle is 15cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 700nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 30% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyacrylic acid and polyester blend preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last polyethylene drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 7:
(1) Bacterial cellulose is prepared by Aerobacter, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 7w/v% glucose, 0.9w/v% calcium carbonate, the water of 0.9w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 9w/v% glucose, 0.2w/v% sodium hydrogen phosphate, 0.1w/v% potassium dihydrogen phosphate, 0.5w/v% yeast extract, the water of 0.5w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 7% the NaOH aqueous solution, and under 100 ℃ temperature, heat 5h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 5% acetum be 1% chitosan-acetic acid solution for 200,000 chitosan with molecular weight.With moisture mass fraction is that 70% Bacterial cellulose covers the surge drum top; It is 5kV that electrostatic spinning voltage is set; Syringe pump speed is 0.1ml/h; The spray webbing syringe needle is 15cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 300nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 40% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyurethane and polyacrylic acid blend preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last politef drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Embodiment 8:
(1) Bacterial cellulose is prepared by azotobacter, and the strain that activation is good places seed culture medium to obtain seed solution.Seed culture medium is the 5w/v% glucose, 0.8w/v% calcium carbonate, the water of 0.8w/v% yeast extract and surplus.Cultured seed solution is inserted in the fermentation medium, obtain Bacterial cellulose.Fermentation medium is the 6w/v% glucose, 0.2w/v% sodium hydrogen phosphate, 0.4w/v% potassium dihydrogen phosphate, 0.3w/v% yeast extract, the water of 0.5w/v% peptone and surplus.To be immersed in the quality percentage composition through the Bacterial cellulose that fermentation culture obtains and be in 8% the NaOH aqueous solution, and under 100 ℃ temperature, heat 6h, the reuse redistilled water washes to neutrality repeatedly.Remove tropina and stick to the residual media on the cellulose membrane.
(2) be that to be dissolved in mass fraction be that to be mixed with mass fraction in 2% acetum be 2% chitosan-acetic acid solution for 150,000 chitosan with molecular weight.With moisture mass fraction is that 60% Bacterial cellulose covers the surge drum top; It is 25kV that electrostatic spinning voltage is set; Syringe pump speed is 0.8ml/h; The spray webbing syringe needle is 15cm apart from the Bacterial cellulose distance, and the chitin fiber diameter that obtains is that the chitin fiber of 250nm evenly covers the Bacterial cellulose surface.The dressing of Bacterial cellulose recombination chitosan fiber washes to neutrality through redistilled water repeatedly, handles through setting-out then that to obtain moisture mass fraction be 50% Bacterial cellulose recombination chitosan fiber moist dressing.
When (3) using Bacterial cellulose recombination chitosan fiber moist dressing is covered or is filled in wound surface; The negative pressure dish is placed in the centre position above dressing; The semipermeable membrane pasted of polyurethane and polyacrylic acid blend preparation is passed the negative pressure dish and covers the dressing top; Semipermeable membrane contacts with the bottom surface concentric discs of negative pressure dish, forms closed system.At last medical silica-gel drainage tube one end is connected the negative pressure dish, an end connects negtive-pressure implement.
Through evidence; Adopt the closed negative pressure drainage device of the above embodiment of the present invention to compare with the closed negative pressure drainage device of present clinical use; The wound healing cycle on average shortens 2-3 days, and the employed expense of this patent is merely 10% of import dressing, greatly reduces patient's financial burden.And a kind of closed negative pressure drainage device of this patent can cut randomly, make up according to shape, the position of wound surface, and is easy to use, have antibiotic, preserve moisture and the negative pressure drainage multi-efficiency.Remedied the deficiency of prior art.
Claims (11)
1. a moist combine dressing is characterized in that: adopt method of electrostatic spinning, chitosan is compounded in the Bacterial cellulose surface, obtain Bacterial cellulose recombination chitosan fiber moist dressing.
2. moist combine dressing according to claim 1; It is characterized in that: adopt method of electrostatic spinning; It is to be mixed with the chitosan-acetic acid solution that mass fraction is 0.5-5% in the 0.1-5% acetum that chitosan is dissolved in mass fraction, and the chitosan-acetic acid solution of formation evenly covers the Bacterial cellulose surface through spinneret orifice.
3. moist combine dressing according to claim 1 and 2; It is characterized in that: adopt method of electrostatic spinning; With moisture mass fraction is that the Bacterial cellulose of 50-90% covers the surge drum top, and it is 5-40kV that electrostatic spinning voltage is set, and syringe pump speed is 0.1-2ml/h; The spray webbing syringe needle is 5-20cm apart from the Bacterial cellulose distance, and chitosan-acetic acid solution is evenly covered the Bacterial cellulose surface through spinneret orifice.
4. moist combine dressing according to claim 1 is characterized in that: said Bacterial cellulose is by one or more outputs in acetobacter xylinum, rhizobium, Sarcina, Rhodopseudomonas, achromobacter, Alcaligenes, Aerobacter or the azotobacter.
5. closed negative pressure drainage device is characterized in that: comprise each described moist combine dressing of claim 1-4, can paste semipermeable membrane, negative pressure dish and connect the drainage tube of negtive-pressure implement.
6. closed negative pressure drainage device according to claim 5 is characterized in that: said moist combine dressing is the Bacterial cellulose recombination chitosan fiber moist dressing of 30-50% for handling the moisture mass fraction that obtains through setting-out.
7. closed negative pressure drainage device according to claim 5 is characterized in that: the said semipermeable membrane of pasting is to have the transparent membrane of folk prescription to airing function.
8. closed negative pressure drainage device according to claim 7 is characterized in that: the said semipermeable membrane of pasting is mixed and made into by one or more of polyurethane, polyacrylic acid or polyester.
9. closed negative pressure drainage device according to claim 5 is characterized in that: said negative pressure dish is made up of hollow cone body medical silicone tube and disc porous medical resin; Wherein the bottom surface of hollow cone body is a concentric discs; Inlay circular porous medical resin in the concentric discs; One tubulose union joint is arranged at the cone top, union joint perpendicular to cone radially and be parallel to bottom surface, there is the inverted cone draw-in groove at the union joint place.
10. closed negative pressure drainage device according to claim 5 is characterized in that: said drainage tube is a hollow pipe; Its material is transparent medical silica-gel, politef, polyethylene or polrvinyl chloride.
11. the described moist combine dressing of claim 1 is used to cure the chronic sexual trauma of not healing, presses acute intractable wound, the application in the curable product of burn and large tracts of land soft tissue defects and/or infection in preparation.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120803A (en) * | 2012-12-29 | 2013-05-29 | 钟春燕 | Preparation method of bacterial cellulose composite chitosan moist antimicrobial dressing |
| CN103205863A (en) * | 2013-04-17 | 2013-07-17 | 钟春燕 | Method for manufacturing bacterial cellulose slow-release dressing |
| CN103285496A (en) * | 2013-05-22 | 2013-09-11 | 苏州爱得科技发展有限公司 | Multi-point vacuum drainage device |
| CN103480027A (en) * | 2012-06-11 | 2014-01-01 | 钟春燕 | Preparation method of bacterial cellulose composite chitosan fiber moist dressing |
| CN103483606A (en) * | 2012-06-11 | 2014-01-01 | 钟春燕 | Preparation method of bacterial cellulose composite polymer superfine fiber material |
| CN103550817A (en) * | 2013-10-29 | 2014-02-05 | 上海昌颌医药科技有限公司 | Bacterial cellulose/chitosan composite sponge dressing and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302486A (en) * | 2008-05-21 | 2008-11-12 | 华中科技大学 | Acetobacter xylinum and method for preparing nano-cellulose skin tissue repair material by using the same |
| CN101638830A (en) * | 2009-08-25 | 2010-02-03 | 江南大学 | Method for preparing nanofibre membrane |
| CN101798756A (en) * | 2010-01-06 | 2010-08-11 | 东华大学 | Method for preparing biomedical material of static self-assembly modified nano fiber |
| CN101922064A (en) * | 2010-07-28 | 2010-12-22 | 武汉纺织大学 | Bacterial cellulose and natural polysaccharide blend fiber and preparation method thereof |
| CN102274574A (en) * | 2011-06-02 | 2011-12-14 | 陈德华 | Surgical wound surface closed negative pressure drainage device |
-
2012
- 2012-05-07 CN CN201210138093.5A patent/CN102631261B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101302486A (en) * | 2008-05-21 | 2008-11-12 | 华中科技大学 | Acetobacter xylinum and method for preparing nano-cellulose skin tissue repair material by using the same |
| CN101638830A (en) * | 2009-08-25 | 2010-02-03 | 江南大学 | Method for preparing nanofibre membrane |
| CN101798756A (en) * | 2010-01-06 | 2010-08-11 | 东华大学 | Method for preparing biomedical material of static self-assembly modified nano fiber |
| CN101922064A (en) * | 2010-07-28 | 2010-12-22 | 武汉纺织大学 | Bacterial cellulose and natural polysaccharide blend fiber and preparation method thereof |
| CN102274574A (en) * | 2011-06-02 | 2011-12-14 | 陈德华 | Surgical wound surface closed negative pressure drainage device |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103483606B (en) * | 2012-06-11 | 2015-11-18 | 钟春燕 | A kind of preparation method of bacterial cellulose composite polymer superfine fiber material |
| CN103483606A (en) * | 2012-06-11 | 2014-01-01 | 钟春燕 | Preparation method of bacterial cellulose composite polymer superfine fiber material |
| CN103480027A (en) * | 2012-06-11 | 2014-01-01 | 钟春燕 | Preparation method of bacterial cellulose composite chitosan fiber moist dressing |
| CN103120803A (en) * | 2012-12-29 | 2013-05-29 | 钟春燕 | Preparation method of bacterial cellulose composite chitosan moist antimicrobial dressing |
| CN103205863A (en) * | 2013-04-17 | 2013-07-17 | 钟春燕 | Method for manufacturing bacterial cellulose slow-release dressing |
| CN103285496A (en) * | 2013-05-22 | 2013-09-11 | 苏州爱得科技发展有限公司 | Multi-point vacuum drainage device |
| CN103285496B (en) * | 2013-05-22 | 2015-10-21 | 苏州爱得科技发展有限公司 | Multi-point vacuum drainage device |
| CN103550817A (en) * | 2013-10-29 | 2014-02-05 | 上海昌颌医药科技有限公司 | Bacterial cellulose/chitosan composite sponge dressing and preparation method thereof |
| CN103876890A (en) * | 2014-03-25 | 2014-06-25 | 深圳市博立生物材料有限公司 | Wet dressing |
| CN106757787A (en) * | 2016-12-20 | 2017-05-31 | 海南大学 | A kind of preparation method of the compound blood compatibility material of shitosan/bacterial cellulose sulfate ester |
| CN106757787B (en) * | 2016-12-20 | 2018-09-04 | 海南大学 | A kind of preparation method of the compound blood compatibility material of chitosan/bacterial cellulose sulfate ester |
| CN107757019A (en) * | 2017-09-21 | 2018-03-06 | 东华大学 | Directional stream-guidance non-woven material and preparation method thereof, use device, application method |
| CN107693213A (en) * | 2017-11-06 | 2018-02-16 | 侯强 | A kind of degradable dressing for Wound-surface negative pressure treatment |
| CN107693213B (en) * | 2017-11-06 | 2024-04-30 | 侯强 | Degradable dressing for wound negative pressure treatment |
| CN111686293A (en) * | 2020-06-18 | 2020-09-22 | 安信纳米生物科技（珠海）有限公司 | Composite dressing and negative pressure drainage device prepared from same |
| CN111686293B (en) * | 2020-06-18 | 2022-04-15 | 安信纳米生物科技（珠海）有限公司 | Composite dressing and negative pressure drainage device prepared from same |
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