CN102628801B - Method detecting harmful compounds in plastic toy based on surface plasma resonance technology - Google Patents
Method detecting harmful compounds in plastic toy based on surface plasma resonance technology Download PDFInfo
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Abstract
The invention discloses a method detecting harmful compounds in a plastic toy based on a surface plasma resonance technology, belonging to the technical field of residue detection of harmful compounds. The method comprises sensing surface aptamer fixing, sample marking, sample detecting and sensing element regenerating; the same sensing chip is used for detecting the residual contents of different harmful compounds; and the method is characterized by comprising the following steps of: connecting the surface of the sensing chip with a thrombin aptamer serving as a marking molecular probe; performing a series of pretreatment on a sample, adding thrombin markers for detecting harmful compounds, mixing, and competitively binding the aptamers which correspond to meal nanoparticle-modified harmful compounds; detecting the content of thrombin by using a surface plasma resonance detector, and indirectly detecting harmful compounds; and regenerating the sensing chip which is used for detecting other harmful compounds. A general detection method with high stability, high repeatability and high sensitivity is provided for detecting harmful compounds in plastic toys, detection of different harmful compounds with the same surface plasma resonance sensing chip is realized, and high generality is achieved.
Description
Technical field
The present invention relates to hazardous compound detection technique in a kind of plastic toy.Particularly based on Applications of surface plasmon resonance, by aptamer and unmarked, mark sample, carry out specificity competition identification, adopt the method for hazardous compound in the sensing element indirect detection plastic toy that thrombin aptamer modifies.
Background technology
Toy is infant's intimate partner always, but also can bring health problem, and hazardous compound is residual is exactly the stealthy killer who hides in plastic toy.In baby toy, relate to the of a great variety of harmful chemical, as adjuvants such as resin and stabilizing agent, plastifier, colorant etc.The bisphenol-A event occurring has in recent years caused the extensive concern of people to harmful chemical in plastic toy, bisphenol-A is the primary raw material of manufacturing epoxy resin, polycarbonate resin, polystyrene resin, is again the stabilizing agent for phenolics, Polyvinylchloride, antioxidant etc.Baby, play in process, lick to eat by mistake by mistake and all likely cause that bisphenol-A moves out from toy, and directly enter in body, infant development, immunity are impacted, even carcinogenic.2011 Nian， European Union have promulgated " new toy safety instruction ", are clearly defined in the toy that EU market sells and must meet new instruction and applied code, show that the relevant trade barrier of European Union upgrade again.Therefore, residual more and more having caused of the hazardous compound in plastic toy both domestic and externally generally paid attention to.
The residual method of traditional detection hazardous compound mainly contains liquid chromatography, gas chromatography, gas-liquid matter combined instrument etc.The advantages such as that although these methods have is highly sensitive, usable range is wide, expensive, process complicated, experimentation is loaded down with trivial details time-consuming, the easy contaminated environment of a large amount of organic reagent used, can not detect online.At present, immuno analytical method is the most general technology of application in hazardous compound fast detecting.Yet existing immunoassay technology used kit and sensor often have selectivity; The shortcomings such as that immune marker also exists is unstable, easy inactivation, have limited further developing of related immune analytical technology.
Applications of surface plasmon resonance because it has high sensitivity, simple to operate, response is fast, need not mark and the advantage such as consumption sample amount is few, be widely used in biochemistry detection field.This technology not only can detect analyte, and can measure the kinetic constant of intermolecular interaction, can provide a kind of easy reliable method for hazardous compound fast detecting in plastic toy; But compare with enzyme linked immunosorbent assay, biology sensor detection method and fluorometry, still exist sensing chip to detect selectivity and the high problem of cost.For example, the instrument BIACore that adopts Surface Plasmon Resonance Technology to detect, its sensors chip SA chip cost is more than 3000 yuan of Renminbi.Therefore, how realizing same sensing chip detects different hazardous compounds and has become surface plasma body resonant vibration detection field hot research problem.
Summary of the invention
In order to solve, sensing selectivity, antibody poor stability, consumable quantity that existing hazardous compound immuno analytical method exists are large, the problem such as involve great expense, greatly improve the detectability of hazardous compound simultaneously, the invention provides a kind of detection method that detects hazardous compound in plastic toy based on surface plasma body resonant vibration instrument.That the method has is general, high stability, high sensitivity, simple operation and other advantages, meets the testing requirement to various hazardous compounds in toy.
Technical scheme of the present invention is: hazardous compound detection method in a kind of plastic toy of application surface plasma resonance instrument, and utilize same sensing chip to detect the content of different hazardous compounds; It is characterized in that comprising the following steps:
(1) sensing chip surface aptamers is fixed: the sensing chip of selecting Streptavidin SA to modify, by passing into 5-50 μ L concentration, be the 1-50 μ g/L thrombin aptamer of mark biotin, on surface plasma sensing chip, fix thrombin aptamer;
(2) hazardous compound to be measured is carried out to fibrin ferment mark, labeling method is different according to determinand group, selects the crosslinked carboxyl of EDC/NHS method and amino or crosslinked two amino of glutaraldehyde method;
(3) the standard model storing solution of phosphate buffer configuration hazardous compound standard solution preparation: with 0.01-0.1mol/L(pH=7.4), storing solution concentration is 0.1ng/mL to 1mg/mL, with phosphate buffer, storing solution is mixed with to the standard solution of variable concentrations, get the standard solution of the unmarked hazardous compound to be measured of variable concentrations and the hazardous compound to be measured of fibrin ferment mark divides after abundant mixing, the aptamers that competition is answered in conjunction with harmful Compound Phase or the aptamers of Nanoparticle Modified, wherein the hazardous compound of fibrin ferment mark should be no less than the adaptive scale of construction adding,
(4) Criterion curve: adopt surface plasma resonance spectrometer to measure, the phosphate buffer of 0.02mol/L of take is measuring basis, by Micropump, pass into 5-100 μ L biased sample to be measured, record surface plasma body resonant vibration instrument spectrogram, by detecting the content of fibrin ferment, indirect detection hazardous compound; Get the surface plasma body resonant vibration spectrogram stationary value of variable concentrations testing sample, drawing curve, and carry out polynomial curve fitting, obtain and return typical curve;
(5) quantitatively detect: the hazardous compound for unknown content detects, and carries out equally according to step (three); Adopt surface plasma body resonant vibration detector to record the spectrogram of hazardous compound; By the corresponding regression curve equation of stationary value substitution of residual component in spectrogram, calculate the concentration value of hazardous compound in plastic toy;
(6) passing into 0.01-0.05mol/L(pH is 2-3) glycocoll-hydrochloric acid buffer solution, rinse sensing chip surface, carry out sensing element regeneration, for other hazardous compound, detect.
Described labeling method comprises EDC/NHS method or glutaraldehyde method.
Can adopt aptamer is identification molecule, and aptamer sequence is directly related with determinand.
Described step (one) sensor surface aptamers is fixed, and comprises the steps:
(1) sensing chip of Streptavidin SA being modified inserts in surface plasma body resonant vibration detector, carries out online sensing surface modification in service aisle;
(2) pass into 0.02mol/L(pH=7.4) phosphate buffer;
(3) passing into 5-50 μ L concentration is the thrombin aptamer solution of 1-50 μ g/L biotin modification, is coupled to online baseline stability;
(4) repeating step (2), to (3), obtains the sensing chip that thrombin aptamer is modified.
Can adopt fibrin ferment mark determinand, identify with the competition of aptamer generation specificity together with unmarked sample.
In described step (three), nano particle can be metal nanoparticle or magnetic nano-particle or macromolecular compound.
In described step (four) and (five) by detecting the content of fibrin ferment, the concentration value of indirect detection hazardous compound, that improves surface plasma sensing chip can reusing, is applicable to the detection of various hazardous compounds.
Beneficial effect of the present invention:
(1) the present invention solves the poor problem of selectivity of hazardous compound detection chip in plastic toy, introduce the fibrin ferment molecule that serves as a mark, adopt biotin labeled aptamers effects on surface plasma resonance sensing chip to modify, the measurement of hazardous compound in plastic toy is converted to the measurement to fibrin ferment, realize the versatility of sensing chip, use same sensing chip to detect different hazardous compounds in plastic toy.The concrete principle that detects is shown in accompanying drawing 1;
(2) the present invention solves chip poor stability problem, adopts aptamers as the general part of chip surface, and regeneration easily.Phase antagonist reusability is strong, has greatly extended the serviceable life of chip, reduces testing cost;
(3) the present invention solves the large problem of reagent consumption, when in employing testing sample, the aptamers of hazardous compound is identified in the danger hazardous compound competition of hazardous compound and fibrin ferment mark, with respect to competition in the past, suppress in conjunction with thought, need not add excessive aptamers, both realize response signal amplification, also saved reagent consumption;
(4) the present invention has improved detection sensitivity, can select the hazardous compound aptamers of Nanoparticle Modified in operation, and while detecting based on surface plasma body resonant vibration, signal significantly increases.
Accompanying drawing explanation
Fig. 1 is that in plastic toy of the present invention, hazardous compound detects schematic diagram; Wherein
for hazardous compound,
for the hazardous compound of fibrin ferment mark,
for the aptamers of hazardous compound,
for the aptamers of fibrin ferment,
for nano particle.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment: selecting in plastic toy two kinds of typical hazardous compound bisphenol-As and monoethanolamine is detected object, and concrete operation step is as follows:
(1) sensor surface aptamers is fixed: the sensing chip of selecting Streptavidin SA to modify is the 30 μ g/L thrombin aptamers of mark biotin by passing into 20 μ L concentration, fixes thrombin aptamer on surface plasma sensing chip;
(2) molecular probe label coupling: determinand is carried out to coupling mark by glutaraldehyde method and fibrin ferment, labeling method, according to determinand group different choice, comprises EDC/NHS method, glutaraldehyde method etc., as bisphenol-A, can adopt EDC/NHS method to be connected with fibrin ferment, stand-by;
(3) standard solution preparation: take bisphenol-A standard items 10 mg, be dissolved in 0.02mol/L(pH=7.4) phosphate buffer is settled to 10 mL, is the standard stock solution of 1 mg/mL; With phosphate buffer, storing solution is mixed with to the standard solution of variable concentrations, concentration, in ng/mL ~ μ g/mL level, is followed successively by 0ng/mL, 500ng/mL, 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, 6 μ g/mL;
(4) Specification Curve of Increasing: be that the unlabelled standard solution of 0 ng/mL fully mixes with appropriate fibrin ferment mark bisphenol-A solution by above-mentioned concentration; The aptamers that adds again the corresponding aptamers of bisphenol-A or Nanoparticle Modified, wherein the bisphenol-A of fibrin ferment mark should be no less than the adaptive scale of construction adding; Adopting surface plasma resonance spectrometer to measure, pass into 0.02mol/L(pH=7.4) phosphate buffer is as measuring basis, then pumps into biased sample to be measured, after surface plasma resonance response value stabilization, recording surface plasma resonance optical spectrum figure;
(5) pass into 0.02mol/L(pH=2.4) glycocoll-hydrochloric acid solution, rinse sensing chip surface, destroy the combination of fibrin ferment and aptamers, complete sensing element regeneration;
(6) concentration, passes into the standard model measuring method to be measured of other concentration (500ng/mL-600 μ g/mL) successively with (4) from low to high, obtains respective surfaces plasma resonance optical spectrum figure; Get the surface plasma spectrogram stationary value of bisphenol-A, drawing standard curve, and carry out polynomial curve fitting, obtain concentration-stationary value and be related to regression curve equation;
(7), after sensing chip regeneration, actual unknown concentration testing sample is fully mixed with appropriate fibrin ferment mark bisphenol-A solution by step (4); In the surface plasma body resonant vibration spectrometer pumping into, record the spectrogram of bisphenol-A simultaneously, determine corresponding stationary value, substitution is typical curve separately, determines each component concentration, and detectability can reach ng/mL ~ μ g/mL level, lower than the highest permission content of national regulation;
(8) again pass into 0.02mol/L(pH=2.4) glycocoll-hydrochloric acid solution, rinse surface plasmon resonance sensing chip, carry out chip regeneration, for the detection of monoethanolamine; Aptamers is transformed to monoethanolamine aptamers, and bisphenol-A-fibrin ferment conjugate becomes monoethanolamine-fibrin ferment conjugate; During monoethanolamine detects, in step (3), the solution concentration of preparation changes 0 μ g/mL into, 100 μ g/mL, 200 μ g/mL, 500 μ g/mL, 1mg/mL, 2mg/mL.Detectability can reach μ g/mL level, lower than the highest permission content of national regulation.
The present invention is accurate and convenient for what narrate, take in an embodiment bisphenol-A and monoethanolamine is described in detail as example, but the present invention is equally applicable to the mensuration of other hazardous compounds in plastic toy, as accurate detection and the qualitative analysis of 2,6-di-tert-butylphenol, aniline etc.Regenerate solution used of sensor surface is selected 0.02mol/L(pH=2.4) glycocoll-hydrochloric acid solution; but can also select salt solusion or weak acid and weak base solution or high ionic strength electrolyte solution; as 0.5 mol/L sodium hydroxide solution or 10 mmol/L glycocoll/hydrochloric acid buffer solutions; destroy the combination of fibrin ferment and aptamers, so foregoing is all within protection domain of the present invention.In addition, according to the method for embodiment 1, detect sample, compare with existing surface plasma detection technique, accuracy improves 2 ~ 10 times, probe life is more than 10 times, the little molecular weight (< 1000Da) materials such as bisphenol-A that existing surface plasma detection technique can not accurately detect, can adopt method of the present invention to obtain testing result accurately.
Claims (6)
1. a detection method for hazardous compound in the plastic toy of application surface plasma resonance technology, utilizes same sensing chip to detect the residual quantity of different hazardous compounds; It is characterized in that comprising the following steps:
(1) sensing chip surface aptamers is fixed: the sensing chip of selecting Streptavidin SA to modify, by passing into 5-50 μ L concentration, be the 1-50 μ g/L thrombin aptamer of mark biotin, on surface plasma sensing chip, fix thrombin aptamer;
(2) hazardous compound to be measured is carried out to fibrin ferment mark, labeling method is different according to determinand group, selects the crosslinked carboxyl of EDC/NHS method and amino or crosslinked two amino of glutaraldehyde method;
(3) standard solution preparation: the standard model storing solution of the phosphate buffer configuration hazardous compound of the pH=7.4 of use 0.01-0.1mol/L, storing solution concentration is 0.1ng/mL to 1mg/mL, with phosphate buffer, storing solution is mixed with to the standard solution of variable concentrations, get the standard solution of the unmarked testing compound of variable concentrations and the hazardous compound to be measured of fibrin ferment mark divides after abundant mixing, the aptamers that competition is answered in conjunction with harmful Compound Phase or the aptamers of Nanoparticle Modified, wherein the hazardous compound of fibrin ferment mark should be no less than the adaptive scale of construction adding,
(4) Criterion curve: adopt surface plasma resonance spectrometer to measure, the phosphate buffer of 0.02mol/L of take is measuring basis, by Micropump, pass into 5-100 μ L biased sample to be measured, record surface plasma body resonant vibration instrument spectrogram, by detecting the content of fibrin ferment, indirect detection hazardous compound; Get the surface plasma body resonant vibration spectrogram stationary value of variable concentrations testing sample, drawing curve, and carry out polynomial curve fitting, obtain and return typical curve;
(5) quantitatively detect: the hazardous compound for unknown content detects, and carries out equally according to step (three); Adopt surface plasma body resonant vibration detector to record the spectrogram of hazardous compound; By the regression curve equation of the recurrence typical curve of stationary value substitution step (four) acquisition of residual component in spectrogram, calculate the concentration value of hazardous compound in plastic toy;
(6) pass into glycocoll-hydrochloric acid buffer solution that the pH of 0.01-0.05mol/L is 2-3, rinse sensing chip surface, carry out sensing element regeneration, for other hazardous compound, detect.
2. method according to claim 1, it is characterized in that adopting aptamer is identification molecule, aptamer sequence is directly related with determinand.
3. method according to claim 1, is characterized in that described step () sensing chip surface aptamers fixes, and specifically comprises the steps:
(1) sensing chip of Streptavidin SA being modified inserts in surface plasma body resonant vibration detector, carries out online sensing surface modification in service aisle;
(2) pass into the phosphate buffer of the pH=7.4 of 0.02mol/L;
(3) passing into 5-50 μ L concentration is the thrombin aptamer solution of 1-50 μ g/L biotin modification, is coupled to online baseline stability;
(4) repeating step (2), to (3), obtains the sensing chip that thrombin aptamer is modified.
4. method according to claim 1, is characterized in that adopting fibrin ferment mark determinand, identifies together with unmarked sample with the competition of aptamer generation specificity.
5. method according to claim 1, is characterized in that in described step (three), nano particle is metal nanoparticle or magnetic nano-particle or macromolecular compound.
6. method according to claim 1, it is characterized in that in described step (four) and (five) by detecting the content of fibrin ferment, the concentration value of indirect detection hazardous compound, that improves surface plasma sensing chip can reusing, is applicable to the detection of various hazardous compounds.
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| CN104155453A (en) * | 2014-07-11 | 2014-11-19 | 天津大学 | Hyaluronic acid modified surface plasma resonance spectrometer chip and preparation method thereof |
| CN111398220B (en) * | 2020-03-31 | 2023-04-07 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Method for sensing and measuring bisphenol A by utilizing graphene multiple signal amplification SPR |
| CN112858225A (en) * | 2021-02-22 | 2021-05-28 | 宁波海歌电器有限公司 | Method for detecting melamine and bisphenol A in milk based on multi-channel optical fiber resonance spectrometer |
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| CN1865994A (en) * | 2006-06-16 | 2006-11-22 | 成都夸常医学工业有限公司 | Analysis method containing organic molecule crosslinked labeling and relative analysis composing |
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