CN107843734A - The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of sex hormone binding globulin - Google Patents
The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of sex hormone binding globulin Download PDFInfo
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- CN107843734A CN107843734A CN201711036915.8A CN201711036915A CN107843734A CN 107843734 A CN107843734 A CN 107843734A CN 201711036915 A CN201711036915 A CN 201711036915A CN 107843734 A CN107843734 A CN 107843734A
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- sex hormone
- binding globulin
- hormone binding
- chemiluminescence
- detection kit
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Abstract
The invention discloses the magnetic microparticle chemiluminescence detection kit and preparation method of a kind of sex hormone binding globulin.The kit includes：Coupling has sex hormone binding globulin detection antibody, sex hormone binding globulin calibration object, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B and the cleaning fluid of the magnetic particle of sex hormone binding globulin capture antibody, acridinium ester label.Kit of the present invention is using Magneto separate chemiluminescence as detection means, in combination with acridinium ester label technology.Direct chemoluminescence method high sensitivity that the present invention establishes, high specificity, accurate quick, detection time is short, and testing result has higher accuracy and repeatability, and the kit is applicable to various luminometer devices.
Description
Technical field
The invention belongs to technical field of immune assay, the immune magnetic particle of specifically a kind of sex hormone binding globulin
Chemiluminescence detection kit and preparation method.
Background technology
Sex hormone binding globulin（sex hormone-binding globulin, SHBG）, also known as testosterone-estradiol
Haptoglobin, the homodimer being made up of Liang Ge identicals subunit, it is a kind of macromolecular globulin, it is mainly thin by liver
Born of the same parents synthesize, and invertibity can occur with sex hormone by the crystal structure of N-terminal and be combined.SHBG major function is transport in human body
Sex hormone simultaneously adjusts its biological effect in the target tissues such as bone, fat, muscle；44%~60% mating type in males
Testosterone is combined with SHBG, and SHBG is mainly responsible for the estrogen of transhipment about 95% in women blood circulation, and its level is to remain male to swash
The indirect indexes of element/estrogen relative equilibrium, and have obvious relation with insulin resistance.Therefore, SHBG can be used as internal testosterone
The supplement of measure, and Endocrine metabolic disease is significant.
Sex hormone binding globulin, which increases, is found in male sexual hypofunction, hyperthyroidism, hepatic sclerosis, Chronic Liver
Scorching, fatty liver.Sex hormone binding globulin reduces and is found in female hirsutism and manlike, Stein-Leventhal syndrome, obesity, first
Shape adenasthenia.
Up to the present, the method for detecting sex hormone binding globulin mainly has：Radio immunoassay
（Radioimmunoassay, RIA）And enzyme-linked immunosorbent assay（ELISA）.Radio immunoassay be based on antigen with
The analysis method that antibody specificity combines, its competition binding principle is similar to elisa assay, although radioimmunoassay is special
Property it is higher, sensitivity is also higher, but its limitation also very significantly, mainly have it is following some：Operating procedure is more, and reagent price is held high
It is expensive, special detection device need to be used, and operating personnel is vulnerable to radiological hazard, while the useful life of kit
It can be limited by the decay period of the radioactive substance marked, therefore the original medicines using RIA measure many in recent years have been used instead more
Other immunoassay methods.Enzyme linked immunosorbent assay (ELISA)（ELISA）Detection technique is exempted from using specific between Ag-Ab
Target molecule in epidemic disease reaction identification biofluid, and utilize target point in the quantitative reaction sample to be tested of the chromogenic reaction of enzyme-substrate
The content of son.Although this method detection it is cheap, quick, sensitivity is also inadequate, be only applicable to micro substance detection and
Identification.Therefore, a kind of method of detection sex hormone binding globulin effective, quick, simple, sensitive, anti-interference is high is established
Tool is of great significance.
The present invention uses method as direct chemoluminescence method, using acridinium ester as chemiluminescent labels with obvious excellent
Gesture, it is mainly manifested in：Reaction does not need catalyst, as long as alkaline environment can be carried out, is swift in response, can complete catching reaction
Caused photon, background luminescence is low, and signal to noise ratio is high, and disturbing factor is few, and reagent stability is good, and system is simple, and exciting liquid cost is low,
Easily and protein bind, and photon yield is not reduced acridinium ester after being coupled.The Magnetism particulate immuno chemistry luminescence method that the present invention establishes is sensitive
Spend height, high specificity, accurate quick, detection time is short, testing result have higher accuracy with it is repeated.
The content of the invention
It is an object of the invention to provide the property that a kind of sensitivity is higher, the reaction time is short, simple to operate, anti-interference is high
The magnetic microparticle chemiluminescence detection kit and preparation method of hormonebinding globulin.
To achieve the above object, the present invention provides following technical scheme：
The magnetic microparticle chemiluminescence detection kit and preparation method of a kind of sex hormone binding globulin, magnetic provided by the present invention
Particulate chemistry luminescent method detects the kit of sex hormone binding globulin, is caught using magnetic particle coupling sex hormone binding globulin
Obtain antibody, acridinium ester label sex hormone binding globulin detection antibody.Kit also include sex hormone binding globulin calibration object,
Chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B and the cleaning fluid of above-mentioned acridinium ester effect.
As the further scheme of the present invention, described magnetic particle can be directly even with sex hormone binding globulin capture antibody
Connection, magnetic particle and Streptavidin can be also coupled, while using biotin labeling sex hormone binding globulin capture antibody.
As the further scheme of the present invention, the surface modification group of the magnetic particle is one or more active function groups
Group, including but not limited to carboxyl, amino, p-toluenesulfonyl or Streptavidin-biotin.
As the further scheme of the present invention, described chemiluminescent labels are acridinium ester, as NSP-DMAE-NHS,
NSP-SA-NHS etc..
As the further scheme of the present invention, described acridinium ester label is sex hormone binding globulin detection antibody.
As the further scheme of the present invention, described acridinium ester label sex hormone binding globulin detects the buffering of antibody
The Na that liquid is pH 8.0-11.0, concentration is 0.01-0.20 mol/L2CO3-NaHCO3Buffer solution.
As the further scheme of the present invention, described capture antibody is monoclonal antibody with detection antibody.
As the further scheme of the present invention, described calibration object be with the Tris containing 0.5-5.0% BSA or PBS or
HEPES buffer solution is matrix, adds the configuration of sex hormone binding globulin sterling and forms, calibration object form is liquid.
As the further scheme of the present invention, described sex hormone binding globulin calibration object solution concentration is respectively：0
nmol/L、0.5 nmol/L、3.0 nmol/L、18.0 nmol/L、108.0 nmol/L、650.0 nmol/L。
As the further scheme of the present invention, described chemiluminescence preexciting liquid A is H2O2And HNO3Mixed liquor, its
Middle H2O2Mass fraction be 0.01-5.0%, HNO3Concentration be 0.01-1.0 mol/L.
As the further scheme of the present invention, described chemiluminescence exciting liquid B is the mixed of Triton X-100 and NaOH
Liquid is closed, wherein Triton X-100 mass fraction is 0.01-2.0%, and NaOH concentration is 0.05-1.0 mol/L.
As the further scheme of the present invention, described cleaning fluid is：PH 7.0-9.0, concentration are 5.0-50.0 mmol/
L Tris or HEPES or other solution, wherein containing the Tween-20 that mass fraction is 0.01-0.25%.
The principle of the present invention is to be combined the high degree of specificity of antibody-antigene reaction with the high sensitivity that acridinium ester lights
Get up, using photon caused by acridinium ester catching reaction to detect production concentration.
The advantage of the invention is that combining magnetic microparticle chemiluminescence technology using double antibody sandwich method, measure sex hormone combines
Globulin content.Acridinium ester has a clear superiority as the direct chemiluminescence of label, is mainly manifested in：Reaction need not urge
Agent, as long as alkaline environment can be carried out, be swift in response, can photon completely caused by catching reaction, background luminescence is low, noise
Than high, disturbing factor is few, and reagent stability is good, and system is simple, and exciting liquid cost is low, and acridinium ester easily and protein bind, and joins
Photon yield is not reduced after knot.
Embodiment
The present invention provides a kind of the magnetic microparticle chemiluminescence detection kit and preparation method of sex hormone binding globulin, is
Make the purpose of the present invention, technical scheme and effect definitely, it is clear, the present invention is described in more detail below.
The present invention provides a kind of the magnetic microparticle chemiluminescence detection kit and preparation method of sex hormone binding globulin, its
In, the kit of magnetic microparticle chemiluminescence method detection sex hormone binding globulin provided by the present invention is even using magnetic particle
Join sex hormone binding globulin capture antibody, acridinium ester label sex hormone binding globulin detection antibody.Kit is also inclusive
Hormonebinding globulin calibration object, chemiluminescence preexciting liquid A, the chemiluminescence exciting liquid B of the effect of above-mentioned acridinium ester and clear
Washing lotion.
Specifically, magnetic particle coupling suspension of the present invention is captured the magnetic of antibody coupling by sex hormone binding globulin
Particle and reagent buffer composition, the concentration of wherein sex hormone binding globulin capture antibody is 0.1-2.0 mg/mL.
Specifically, the kernel of magnetic bead of the present invention is ferroso-ferric oxide, described magnetic particle can directly with sex hormone knot
Globulin capture antibody coupling is closed, magnetic particle and Streptavidin can be also coupled, while use biotin labeling sex hormone knot
Close globulin capture antibody.Magnetic bead can influence accuracy using the endless bulk deposition of preceding solid phase, therefore should select dispersiveness during selection
It is good, the slow magnetic bead of sinking speed.
Specifically, for the present invention when preparing magnetic particle suspension, the coupled antibody buffer solution is pH 5.0-6.0, concentration
For 20-200 mmol/L MES buffer solutions.
Specifically, for the present invention when preparing magnetic particle suspension, the Block buffer is the buffer solution containing 1%BSA.
Specifically, the present invention prepare mark liquid-phase reagent when, the mark buffer solution is that pH 8.0-11.0, concentration are
0.01-0.20 mol/L Na2CO3-NaHCO3Buffer solution.
Specifically, calibration object of the present invention is to be with the Tris containing 0.5-5.0% BSA or PBS or HEPES buffer solution
Matrix, add the configuration of sex hormone binding globulin sterling and form, calibration object form is liquid.
Specifically, chemiluminescence preexciting liquid A of the present invention is H2O2And HNO3Mixed liquor, wherein H2O2Quality
Fraction is 0.01-5.0%, HNO3Concentration be 0.01-1.0 mol/L.
Specifically, chemiluminescence exciting liquid B of the present invention is Triton X-100 and NaOH mixed liquor, wherein
Triton X-100 mass fraction is 0.01-2.0%, and NaOH concentration is 0.05-1.0 mol/L.
Specifically, cleaning fluid of the present invention is：PH 7.0-9.0, concentration be 5.0-50.0 mmol/L Tris or
HEPES or other solution, wherein containing the Tween-20 that mass fraction is 0.01-0.25%.
Below by embodiment, the present invention is described in detail.
Embodiment 1：A kind of establishment of the magnetic microparticle chemiluminescence kit of described detection sex hormone binding globulin and
Preparation method, comprise the following steps：
1. the establishment of kit：
A kind of magnetic microparticle chemiluminescence kit for detecting sex hormone binding globulin is set up, it is contained following component：
The sex hormone binding globulin monoclonal antibody of magnetic particle coupling；
Another plant of sex hormone binding globulin monoclonal antibody of acridinium ester label；
Sex hormone binding globulin serial standards solution；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Cleaning fluid.
2. coupling has the preparation of the magnetic particle suspension of sex hormone binding globulin monoclonal antibody
（1）1 mg carboxyls magnetic particle is taken in 0.5 mL centrifuge tubes, adds the MES buffer solutions that 200 μ L concentration are 0.1 mol/L,
It is vortexed and mixes, be placed on magnetic frame, standing 5 min makes magnetic particle be separated with liquid, supernatant discarding, washs 3 times, adds 200
μ L MES（PH is 6.0）Buffer solution, it is vortexed.
（2）15 μ g sex hormone binding globulin monoclonal antibody is added, it is 150 to make the mol ratio of carboxyl and antibody:1,
It is vortexed, revolving reaction pipe, is incubated at room temperature 30 min.
（3）The coupling reagent EDC that 10 μ L concentration are 10 mg/mL is added, in being vortexed on rotatable reactor, is incubated at room temperature 2
h。
（4）Take 200 glycine buffers of the μ L containing 1%BSA（Concentration is 50 mmol/L）Closed, time 1h.
（5）Supernatant is removed, adds 200 μ L cleaning buffer solution（TBS+0.05%Tween-20）, wash 3 times.
（6）The above-mentioned magnetic particle suspension prepared is placed in 500 μ L and preserves liquid（It is 300 mmol/L glycine, 2% sweet
Oil, 5% sucrose）In, 2-8 DEG C of preservation.
3. the preparation of the sex hormone binding globulin monoclonal antibody liquid-phase reagent of acridinium ester label
（1）Purify sex hormone binding globulin monoclonal antibody：100 μ g sex hormone binding globulin monoclonal antibodies are placed in
In bag filter, and bag filter is placed in the mark buffer solution not less than 1 L and dialysed, during which buffer fluid exchange 5 times, last time
Dialysed overnight, the Na that mark buffer solution is pH 9.8, concentration is 50 mmol/L2CO3-NaHCO3Buffer solution.
（2）1.7 mg acridinium ester NSP-DMAE-NHS is weighed, is dissolved in 447 μ L anhydrous dimethyl formamides（DMF）In,
It is made into 6.5 mmol/L NSP-DMAE-NHS DMF solutions.
（3）It will be placed in 500 μ L centrifuge tubes, add through the sex hormone binding globulin monoclonal antibody solution after dialysis
Enter 200 μ L mark buffer solutions, then add 759 μ L, 6.5 mmol/L NSP-DMAE-NHS DMF solutions（Lucifuge is reacted）
The mol ratio for making acridinium ester and sex hormone binding globulin monoclonal antibody is 7.4:1, vibration mixes, and reacts 1 h at room temperature, adds
Enter the lysine that 100 μ L concentration are 10 g/L, stand 15 min, make mark reaction terminating.
（4）Label NSP-DMAE-NHS-Ab and free NSP-DMAE-NHS passes through Sephadex G-50 posts（1×
25cm）Separation, the PBS for being 0.1mol/L with purification buffer pH 6.3, concentration are balanced and are eluted chromatographic column.
（5）Protein peak is detected with chromatograph in separation process, the chemiluminescence intensity and 280nm for measuring efflux respectively are inhaled
Shading value.
（6）The eluent that shading value is high and absorbance is big is collected, stored frozen is dispensed after adding 1% BSA.
4. the preparation of sex hormone binding globulin calibration object
Sex hormone binding globulin sterling is configured into sign concentration with the Tris-HCl buffer solutions containing 2% BSA is：0
Nmol/L, 0.5 nmol/L, 3.0 nmol/L, 18.0 nmol/L, 108.0 nmol/L, 650.0 nmol/L totally 6 concentration
Calibration object Grad.
5. chemiluminescence exciting liquid A, B preparation
（1）Chemiluminescence preexciting liquid A is by H2O2And HNO3Composition.Wherein, wherein H2O2Mass fraction be 1.5%, HNO3It is dense
Spend for 0.1 mol/L, be distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
（2）Chemiluminescence exciting liquid B is made up of Triton X-100 and NaOH mixed liquor.Wherein, Triton X-100
Mass fraction be 1.0%, NaOH concentration be 0.4 mol/L, be distributed into 20 mL/ branch with brown bottle, 2-8 DEG C saves backup.
6. the preparation of cleaning fluid
In the beaker for weighing 3.05g Tris, 8.775g NaCl to 1000 mL, it is 0.05% Tween- to add 1mL mass fractions
20, stir and evenly mix, constant volume, saved backup after pH is adjusted into 7.6.
Embodiment 2：Detected using the magnetic microparticle chemiluminescence detection kit of described sex hormone binding globulin
The step of be：
（1）The μ L of sample to be tested 50 are added in reaction cup, add the magnetic particle coupling μ L of suspension 150, vibration mixes, 37 DEG C
It is incubated 8 min.
（2）Separation, clean 3 times, the reaction vessel after washing, which is fully vibrated, makes magnetic particle scatter.
（3）The μ L of acridinium ester label 150 are added into reaction cup, vibration mixes, 37 DEG C of 7 min of incubation.
（4）Separation, clean 3 times, the reaction vessel after washing, which is fully vibrated, makes magnetic particle scatter.
（5）100 μ L chemiluminescence exciting liquid B are added after adding 100 μ L chemiluminescence preexciting liquid A, 1 s, measure it
Relative luminous intensity, the content of sample Sex Hormones haptoglobin luminous intensity proportion relation corresponding thereto.
Embodiment 3：The performance indications of kit
（1）Sensitivity for analysis
By the use of 0 nmol/L calibration objects in kit as sample to be tested, replication 20 times, the RLU values of 20 measurement results are drawn
（Relative light unit）, calculate its average value（M）And standard deviation（SD）, RLU values corresponding to M+2SD are drawn, according to zero-dose school
Concentration-RLU values result between quasi- product and adjacent calibration object carries out 2 regression fits and draws linear function, by M+2SD's
RLU values are brought into above-mentioned equation, obtain corresponding concentration value.By minimum detection limit detection method in detection scheme, it is repeated 3 times
Experiment, it is 0.3 nmol/L to the sensitivity for analysis of sex hormone binding globulin finally to obtain this kit.
（2）Precision
It is (50.0 ± 5.0) nmol/L and (300.0 ± 30.0) nmol/ with sex hormone binding globulin kit test concentrations
L sample, each concentration retest 10 times, calculate kit precision, the results showed that kit CV is respectively less than 5%.
（3）Stability
Sex hormone binding globulin detection kit is taken to carry out normal storage stability test, 2-8 DEG C is placed respectively temporally 1,
Detected within 3,5,7,9,11,12,13,14,15 months；Uncap stability test respectively by 2-8 DEG C place 0 day, 7 days, 14 days,
It is measured within 16 days, 18 days, 20 days, 22 days, 24 days, 26 days, 28 days, 30 days, 32 days.As a result sex hormone binding globulin is shown
Detection kit is stored in 2-8 DEG C, in light protected environment, and the term of validity is 12 months.Uncap and be stored in 2-8 DEG C, in light protected environment, have
The effect phase is 30 days.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (9)
1. the magnetic microparticle chemiluminescence detection kit and its composition of a kind of sex hormone binding globulin, it is characterised in that：Including
Coupling has the sex hormone binding globulin detection of the magnetic particle suspension of sex hormone binding globulin capture antibody, acridinium ester label
Antibody, calibration object, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B and cleaning fluid.
2. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：The kernel of described magnetic bead is ferroso-ferric oxide or di-iron trioxide, and the surface modification group of magnetic particle is
One or more activity functional groups, including but not limited to carboxyl, amino, p-toluenesulfonyl or Streptavidin-biotin.
3. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described magnetic particle directly can capture antibody coupling with sex hormone binding globulin, or by magnetic particle and chain
Mould Avidin coupling, while using biotin labeling sex hormone binding globulin capture antibody.
4. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described chemiluminescent labels are acridinium ester, such as NSP-DMAE-NHS, NSP-SA-NHS.
5. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described acridinium ester label is sex hormone binding globulin detection antibody.
6. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described capture antibody is monoclonal antibody with detection antibody.
7. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described calibration object is using the buffer solution containing BSA as matrix, adds sex hormone binding globulin sterling
Configure the calibration object solution of the series concentration gradient formed.
8. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into, it is characterised in that：Described chemiluminescence preexciting liquid A is by H2O2And HNO3Mixed liquor composition, chemiluminescence exciting liquid B
It is made up of Triton X-100 and NaOH mixed liquor.
9. the magnetic microparticle chemiluminescence detection kit and its group of a kind of sex hormone binding globulin according to claim 1
Into comprising the following steps：A certain amount of sample to be tested is added in reaction cup, magnetic particle coupling suspension is added, mixes, 37
DEG C be incubated, add cleaning fluid remove supernatant；Acridinium ester label is added according to certain ratio afterwards, 37 DEG C of incubations, is added clear
Washing lotion removes supernatant, adds chemiluminescence preexciting liquid A and exciting liquid B, determines corresponding luminous intensity RLU/s.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108896538A (en) * | 2018-06-21 | 2018-11-27 | 上海彧成生物科技有限公司 | A kind of creatinine chemiluminescence immune detection reagent kit and each component preparation method |
| CN112147337A (en) * | 2020-08-19 | 2020-12-29 | 德赛诊断系统(上海)有限公司 | Cysteine-rich protein 61 high-sensitivity chemiluminescence detection kit and preparation and application thereof |
| CN112630451A (en) * | 2021-01-25 | 2021-04-09 | 安士(广州)医疗科技有限公司 | Preparation method and application of chemiluminescence detection kit for quantitatively detecting concentration of TEX101 |
| CN113588967A (en) * | 2021-08-03 | 2021-11-02 | 湖南永和阳光生物科技股份有限公司 | Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein |
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2017
- 2017-10-31 CN CN201711036915.8A patent/CN107843734A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108896538A (en) * | 2018-06-21 | 2018-11-27 | 上海彧成生物科技有限公司 | A kind of creatinine chemiluminescence immune detection reagent kit and each component preparation method |
| CN112147337A (en) * | 2020-08-19 | 2020-12-29 | 德赛诊断系统(上海)有限公司 | Cysteine-rich protein 61 high-sensitivity chemiluminescence detection kit and preparation and application thereof |
| CN112630451A (en) * | 2021-01-25 | 2021-04-09 | 安士(广州)医疗科技有限公司 | Preparation method and application of chemiluminescence detection kit for quantitatively detecting concentration of TEX101 |
| CN113588967A (en) * | 2021-08-03 | 2021-11-02 | 湖南永和阳光生物科技股份有限公司 | Magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein |
| CN113588967B (en) * | 2021-08-03 | 2024-02-20 | 湖南永和阳光生物科技股份有限公司 | Magnetic particle chemiluminescence detection kit for growth stimulation expressed gene 2 protein |
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Application publication date: 20180327 |