CN102627700A - Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis - Google Patents
Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis Download PDFInfo
- Publication number
- CN102627700A CN102627700A CN2012101226171A CN201210122617A CN102627700A CN 102627700 A CN102627700 A CN 102627700A CN 2012101226171 A CN2012101226171 A CN 2012101226171A CN 201210122617 A CN201210122617 A CN 201210122617A CN 102627700 A CN102627700 A CN 102627700A
- Authority
- CN
- China
- Prior art keywords
- hydrolysis
- scallop
- raw material
- internal organ
- shirt rim
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention provides a process route for extracting polysaccharide in a skirt edge and viscera of scallop through hydrolysis, which comprises the process procedures of raw material drying, homogenate, hydrolysis, separation, ethanol precipitation, decoloration and spray drying. An obtained product can serve as a product and also can serve as an auxiliary material applied to other functional foods. The method for extracting the polysaccharide in the skirt edge and viscera of the scallop through the hydrolysis has the advantages of fully utilizing bay scallop processing waste to solve the environment problem and also providing ideas for the application of leftovers. The manufactured product is high in yield and low in cost. The method for extracting the polysaccharide in the skirt edge and viscera of the scallop through the hydrolysis has important significance to both the improvement of the comprehensive development and utilization of the processing of bay scallops and sustainable development and social progress of sea products of our country.
Description
Technical field
The present invention relates to a kind of hydrolysis method and extract the method for polysaccharide in bay scallop shirt rim and the internal organ, belong to bay scallop by product processing technique field.
Background technology
China's aquaculture development is rapid since the eighties in 20th century, and the breed of scallop is quite universal, and the processing and the export volume of freezing Bei Zhu and dried scallop post are very big, becomes the fishery products of the large outlet of China.And in the process of processing shellfish post, produced a large amount of tankage, wherein scallop processing waste (comprising internal organ) accounts for 30% of whole scallop.Wherein the small portion scallop processing waste is used as the shrimp feed and undersells, and most of scallop processing waste is abandoned in the seashore and let alone to rot, and causes the great wasting of resources and environmental pollution.In these wastes, contain and have multi-functional active polysaccharide.Polysaccharide is a family macromolecule carbohydrate polymer; Extensively be present in animal, plant, mikrobe and the sea life; The identification of adjusting, cell and the cell of it and immunologic function, the transportation of intercellular substance, Clinics and Practices of cancer etc. all have confidential relation.That polysaccharide in the scallop has is hypoglycemic, anticoagulation, antitumor, prevent cardiovascular and cerebrovascular diseases and a series of special nourishing functions such as antiviral.Hence one can see that, and the technology of ammonia polyose of candy is extracted in research from bay scallop shirt rim and internal organ, China's sea-food industry is developed new product, and widens market, makes full use of scallop by product resource, improves its economic benefit, has far reaching significance.In the prior art: the patent of " process for extracting comb shell polysaccharide " by name that the patent No. has been 200510047410.2 one Chinese patent application; Processing condition are to extract enzyme with enzymatic hydrolysis; Can from the smooth shellfish of shrimp, extract polysaccharide; But utilize enzyme to be hydrolyzed, conditional request is relatively harsher, operates more loaded down with trivial details.Liu Xiaoyan etc. utilize hydrolysis method to extract the freshwater shellfish polysaccharide, are research object with the river snail, from river snail, extract polysaccharide; Optimum extraction condition is an alcohol precipitation concentration 75%, 60 ℃ of extraction temperatures, and lixiviate was than 1: 2; Extraction time 6 h do not report shellfish pre-treatment and aftertreatment technology condition.
Summary of the invention
It is raw material with scallop body and internal organ that the object of the invention aims to provide a kind of, utilizes hydrolysis method to combine related process, improves polysaccharide extract rate to greatest extent, and reduces the novel process of the cost that extracts polysaccharide.
Technical scheme of the present invention is that scallop splanchna and shirt rim are carried out the raw material processing, through the product that obtains its polysaccharide of hydrolysis extraction and purifying.Be characterized in:
(1) raw material is handled: is scallop processing depleted shirt rim, back or internal organ raw material, and cleaning drains, and removes impurity such as silt.In 80 ℃ dry 6-10 hour down, then 60 ℃ dry 36 hours down, crushed after being dried becomes the 100-200 order,, gets in the water-bath at 70 ℃ as solvent with normal hexane, carries out degreasing with soxhlet extraction.
(2) homogenate: after the degreasing, according to the quality of raw material after the degreasing, get the water of twice, be divided into isopyknic two parts, a copy of it adds in the raw material, carries out homogenate with tissue mashing machine, obtain homogenate after, add the water of another part again.
(3) hydrolysis: put into homogenate in 60 ℃ of water-baths of temperature and to be hydrolyzed, hydrolysis 6 hours, spinning (3500r/min 15min), throw out hydrolysis 6 hours in 60 ℃ of water-baths again, centrifugation again merges supernatant.
(4) alcohol precipitation: get polysaccharide extraction liquid and carry out vacuum filtration, under 0.1Mp, 60 ℃ of conditions, carry out vacuum concentration again, after concentrating; Adding absolute ethyl alcohol to alcohol concn is 60%, and the limit edged stirs, and continues evenly to stir 2h then; Put it into again in the refrigerating chamber (4 ℃) and leave standstill 24h; Carry out spinning 15min (3500r/min) at last, abandon supernatant, get dope.
(5) decolouring: get sedimentary dope; According to the dope volume; The pure water that adds 20 times adds isopyknic 1% gac (activation 2h under 65 ℃ of water bath condition in advance), continuous whip attachment 30min under whisking appliance; Filter (zeyssatite helps filter) and remove impurity such as gac and pigment, get clear liquid.
(6) spraying drying: with the clear liquid concentrating under reduced pressure, carry out spraying drying then, get the tiny solid powder, be polysaccharide product.
Specific embodiments
Case 1:
(1) raw material is handled: is depleted shirt rim, scallop processing back raw material, and cleaning drains, and removes impurity such as silt.In 80 ℃ dry 6-10 hour down, then 60 ℃ dry 36 hours down, crushed after being dried becomes the 100-200 order,, gets in the water-bath at 70 ℃ as solvent with normal hexane, carries out degreasing with soxhlet extraction.
(2) homogenate: after the degreasing, according to the quality of raw material after the degreasing, get the water of twice, be divided into isopyknic two parts, a copy of it adds in the raw material, carries out homogenate with tissue mashing machine, obtain homogenate after, add the water of another part again.
(3) hydrolysis: put into homogenate in 60 ℃ of water-baths of temperature and to be hydrolyzed, hydrolysis 6 hours, spinning (3500r/min 15min), throw out hydrolysis 6 hours in 60 ℃ of water-baths again, centrifugation again merges supernatant.
(4) alcohol precipitation: get polysaccharide extraction liquid and carry out vacuum filtration, under 0.1Mp, 60 ℃ of conditions, carry out vacuum concentration again, after concentrating; Adding absolute ethyl alcohol to alcohol concn is 60%, and the limit edged stirs, and continues evenly to stir 2h then; Put it into again in the refrigerating chamber (4 ℃) and leave standstill 24h; Carry out spinning 15min (3500r/min) at last, abandon supernatant, get dope.
(5) decolouring: get sedimentary dope; According to the dope volume; The pure water that adds 20 times adds isopyknic 1% gac (activation 2h under 65 ℃ of water bath condition in advance), continuous whip attachment 30min under whisking appliance; Filter (zeyssatite helps filter) and remove impurity such as gac and pigment, get clear liquid.
(6) spraying drying: with the clear liquid concentrating under reduced pressure, carry out spraying drying then, get the tiny solid powder, be polysaccharide product.
Case 2:
(1) raw material is handled: is depleted shirt rim, scallop processing back raw material, and cleaning drains, and removes impurity such as silt.In 80 ℃ dry 6-10 hour down, then 60 ℃ dry 36 hours down, crushed after being dried becomes the 100-200 order,, gets in the water-bath at 70 ℃ as solvent with normal hexane, carries out degreasing with soxhlet extraction.
(2) homogenate: after the degreasing, according to the quality of raw material after the degreasing, get the water of twice, be divided into isopyknic two parts, a copy of it adds in the raw material, carries out homogenate with tissue mashing machine, obtain homogenate after, add the water of another part again.
(3) hydrolysis: put into homogenate in 60 ℃ of water-baths of temperature and to be hydrolyzed, hydrolysis 6 hours, spinning (3500r/min 15min), throw out hydrolysis 6 hours in 60 ℃ of water-baths again, centrifugation again merges supernatant.
(4) alcohol precipitation: get polysaccharide extraction liquid and carry out vacuum filtration, under 0.1Mp, 60 ℃ of conditions, carry out vacuum concentration again, after concentrating; Adding absolute ethyl alcohol to alcohol concn is 60%, and the limit edged stirs, and continues evenly to stir 2h then; Put it into again in the refrigerating chamber (4 ℃) and leave standstill 24h; Carry out spinning 15min (3500r/min) at last, abandon supernatant, get dope.
(5) decolouring: get sedimentary dope; According to the dope volume; The pure water that adds 20 times adds isopyknic 1% gac (activation 2h under 65 ℃ of water bath condition in advance), continuous whip attachment 30min under whisking appliance; Filter (zeyssatite helps filter) and remove impurity such as gac and pigment, get clear liquid.
(6) spraying drying: with the clear liquid concentrating under reduced pressure, carry out spraying drying then, get the tiny solid powder, be polysaccharide product.
Claims (5)
1. extract polysaccharide method in bay scallop shirt rim and the internal organ according to the claim hydrolysis method, it is characterized in that the raw material in the said raw material processing is bay scallop internal organ and bay scallop shirt rim.
2. extract polysaccharide method in bay scallop shirt rim and the internal organ according to the described hydrolysis method of claim; It is characterized in that raw material is following dry 6-10 hour at 80 ℃ in the said raw material processing; Descended dry 36 hours at 60 ℃ then, crushed after being dried becomes the 100-200 order, carries out degreasing again.
3. extract polysaccharide method in bay scallop shirt rim and the internal organ according to the described hydrolysis method of claim, the condition that it is characterized in that said hydrolysis is the quality according to getting raw material, adds 2 times water, in 60 ℃ of water-baths, is hydrolyzed, and hydrolysis time is 6 hours.
4. extract polysaccharide method in bay scallop shirt rim and the internal organ according to the described hydrolysis method of claim; It is characterized in that said alcohol precipitation is after concentrating; Add ethanol, the limit edged stirs, and final alcohol concn is 60%; Put under 4 ℃ of conditions behind the continuation stirring 2h and leave standstill 24h, carry out spinning 15min (3500r/min) at last and separate.
5. extract polysaccharide method in bay scallop shirt rim and the internal organ according to the described hydrolysis method of claim; It is characterized in that said decolouring is after alcohol precipitation and spinning; According to obtaining the water that sedimentary quality adds 20 times; Add 1% gac that equates with the throw out quality again, continuous whip attachment 30min under whisking appliance filters (zeyssatite helps filter) and removes impurity such as gac and pigment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101226171A CN102627700A (en) | 2012-04-25 | 2012-04-25 | Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101226171A CN102627700A (en) | 2012-04-25 | 2012-04-25 | Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102627700A true CN102627700A (en) | 2012-08-08 |
Family
ID=46586113
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012101226171A Pending CN102627700A (en) | 2012-04-25 | 2012-04-25 | Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102627700A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103172760A (en) * | 2013-03-26 | 2013-06-26 | 大连工业大学 | Method for preparing sulfated glycogen based on oysters or scallops |
| CN104804104A (en) * | 2015-03-11 | 2015-07-29 | 河北农业大学 | Method for extracting polysaccharide in low heavy metal from aprons or viscera of scallops |
| CN105131143A (en) * | 2015-09-29 | 2015-12-09 | 江苏锦宇环境工程有限公司 | Preparing method of scallop viscera oligosaccharide |
| CN107955080A (en) * | 2017-11-20 | 2018-04-24 | 淮阴工学院 | It is a kind of that there is the anti-oxidant and method of the white shellfish acidic polysaccharose of liver protection effect from Bai Beizhong extractions |
| CN108477619A (en) * | 2018-03-16 | 2018-09-04 | 青岛大学 | A kind of preparation method of compound amino acid chelate calcium |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06279292A (en) * | 1993-02-12 | 1994-10-04 | Aomori Pref Gov | Production of glycogen having physiological activity and physiological activity |
| CN1749281A (en) * | 2005-10-11 | 2006-03-22 | 大连轻工业学院 | Process for extracting comb shell polysaccharide |
| CN1827755A (en) * | 2006-04-13 | 2006-09-06 | 大连轻工业学院 | Preparation method of scallop splanchna active components |
| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101503721A (en) * | 2008-11-27 | 2009-08-12 | 浙江珍世堂生物科技有限公司 | Method for extracting swan-mussel polysaccharide |
-
2012
- 2012-04-25 CN CN2012101226171A patent/CN102627700A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06279292A (en) * | 1993-02-12 | 1994-10-04 | Aomori Pref Gov | Production of glycogen having physiological activity and physiological activity |
| CN1749281A (en) * | 2005-10-11 | 2006-03-22 | 大连轻工业学院 | Process for extracting comb shell polysaccharide |
| CN1827755A (en) * | 2006-04-13 | 2006-09-06 | 大连轻工业学院 | Preparation method of scallop splanchna active components |
| CN101177460A (en) * | 2007-12-05 | 2008-05-14 | 浙江工商大学 | Method for extracting water-soluble soybean polysaccharide from soya bean waste and soybean dregs |
| CN101503721A (en) * | 2008-11-27 | 2009-08-12 | 浙江珍世堂生物科技有限公司 | Method for extracting swan-mussel polysaccharide |
Non-Patent Citations (4)
| Title |
|---|
| 佟海菊等: ""海湾扇贝多糖水提工艺研究"", 《河北农业大学学报》 * |
| 季宇彬: "《中药多糖的化学与药理》", 30 June 2005 * |
| 王长云等: ""扇贝边中酸性粘多糖的提取"", 《青岛海洋大学学报》 * |
| 闫雪等: ""虾夷扇贝内脏多糖SVP-12 的分离纯化及性质研究"", 《食品与发酵工业》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103172760A (en) * | 2013-03-26 | 2013-06-26 | 大连工业大学 | Method for preparing sulfated glycogen based on oysters or scallops |
| CN104804104A (en) * | 2015-03-11 | 2015-07-29 | 河北农业大学 | Method for extracting polysaccharide in low heavy metal from aprons or viscera of scallops |
| CN105131143A (en) * | 2015-09-29 | 2015-12-09 | 江苏锦宇环境工程有限公司 | Preparing method of scallop viscera oligosaccharide |
| CN107955080A (en) * | 2017-11-20 | 2018-04-24 | 淮阴工学院 | It is a kind of that there is the anti-oxidant and method of the white shellfish acidic polysaccharose of liver protection effect from Bai Beizhong extractions |
| CN107955080B (en) * | 2017-11-20 | 2020-04-24 | 淮阴工学院 | A method for extracting acidic polysaccharide from Bulbus Fritillariae Cirrhosae with antioxidant and hepatoprotective effects |
| CN108477619A (en) * | 2018-03-16 | 2018-09-04 | 青岛大学 | A kind of preparation method of compound amino acid chelate calcium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105622781B (en) | A kind of method of chitin in eutectic ionic liquid extraction shrimp and crab shells | |
| CN104086667A (en) | Method for preparing pectin from citrus fruit peel residues by employing ultrasonic-assisted extraction | |
| CN102627700A (en) | Method for extracting polysaccharide in skirt edge and viscera of scallop through hydrolysis | |
| CN109608559A (en) | A method of extracting active polysaccharide from seaweed | |
| CN106281638B (en) | A kind of method that camellia seed oil and oil tea saponin and camellia seed meal feed are extracted from camellia seed kernel | |
| CN102524714B (en) | Comprehensive utilization method for konjac | |
| CN102432664A (en) | Method for extracting theasaponin from tea seed pulp | |
| CN106957374A (en) | A kind of method that fucoidin and algin are extracted from algae | |
| CN102077898A (en) | Peanut protein concentrate preparation method | |
| CN101851300A (en) | Process for extracting chondroitin sulfate | |
| CN105249464A (en) | Preparation method of shrimp peptides chelated calcium | |
| CN107411081A (en) | A kind of Methods of Extraction of Tea-polyphenols | |
| CN103589185A (en) | Method for extracting pigment and polyphenol from water chestnut peels | |
| CN103694374A (en) | Method for extracting agar, fucoidin and protein from gracilaria as raw material by using enzyme process | |
| CN102295984A (en) | Method and device for producing fruit oil of cornus wilsoniana | |
| CN102850466A (en) | Production process for chondroitin sulfate from yak bone | |
| CN108998491A (en) | A method of extracting protein in the peony seeds dregs of rice | |
| CN103864945A (en) | Method for intensively extracting polysaccharides from rice culture medium for cordyceps militaris | |
| CN105463040A (en) | Method for raising yield of xylooligosaccharide | |
| CN102040665A (en) | Method for extracting sea cucumber polysaccharide | |
| CN102532334A (en) | Method for producing flaxseed mucilage | |
| CN106011181B (en) | Method for preparing fuel ethanol from kitchen garbage | |
| CN101756295A (en) | Method for preparing kelp dietary fiber by ultrasonic enzymolysis | |
| CN102477101A (en) | Method for extracting polysaccharide from Argopecten irradians | |
| CN105039486A (en) | Method for extracting ferulic acid from wheat straw by biotechnology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120808 |