CN102626176B - Composite high peptidergic biological protein feed and preparation method thereof - Google Patents

Abstract

The invention discloses composite high peptidergic biological protein feed. The invention also discloses a preparation method of the protein feed. Blood meal and palm meal subjected to peptide are reasonably mixed to prepare the high peptidergic biological protein feed which has high peptidergic biological protein and is a micro-ecological preparation rich in high small peptide, high energy and high protein. The quality and a feeding effect of the composite high peptidergic biological protein feed are very close to that of fermented bean meal and even are better than that of the fermented bean meal. According to the feed and the preparation method thereof, not only is the global difficulty that the protein is in shortage effectively alleviated, but also the feed is easy to popularize rapidly in feed enterprises because of low production cost and remarkable prebiotics actions, and the process for prebiotics to gradually substitute antibiotics can be accelerated.

Description

A kind of compound high Toplink biological protein feedstuff and preparation method

Technical field

The present invention discloses a kind of compound high Toplink biological protein feedstuff, the invention also discloses the preparation method of this protein feed, belongs to the biological feedstuff technical field.

Background technology

Current, in China's feedstuff industry, along with " feed and feed addictive management rules " promulgate and execution requirements, forbid the drug abuse additive, the feed addictive selectable range is more and more less, and the probiotics of lived short long agent and health-care agent is maximum bright spot.

Blood meal is as feed, and its advantage is aboundresources and cheap, and protein content is up to more than 80%, and lysine content exceeds more than two times than dregs of beans, and arginine content is low, is the primary raw material of balance low-quality grouts lysine.Its shortcoming is that digestibility is lower, and palatability is poor, and the amino acid Compositional balance is poor.And palm kernel meal is as feed, its advantage is aboundresources and cheap, without mouldiness and side effect, nutritious equilibrium, the gal4 amino acid balance is good, be rich in vitamin, trace element, the most important using value in feed is that crude fat content is very high, usually it is classified as energy feed and uses.Its shortcoming is that crude fiber content is higher, and palatability is relatively poor.So blood meal and palm kernel meal separately as feed addictive, all exist separately shortcoming and deficiency, and simply will both mix use, also exist.

Summary of the invention

The invention provides a kind of compound high Toplink biological protein feedstuff, is mixed feed with blood meal and palm kernel meal by engineering bacteria production, has solved the shortcoming that exists separately.

The present invention further discloses the preparation technology of compound high Toplink biological protein feedstuff, be adapted to suitability for industrialized production.

Compound high Toplink biological protein feedstuff disclosed by the invention,It is characterized in that mainly by following raw materials by weight portion than making:

Blood meal (50-80) part, palm kernel meal (20-50) part, lysozyme bacterium (0.5-5) part, cellulase bacterium (0.5-3) part, lactobacillus acidophilus (0.5-2) part.

The production technology of compound high Toplink biological protein feedstuff provided by the invention,It comprises the following steps:

1) blood meal is mixed with palm kernel meal;

2) under 30-45 ℃ of condition, the material that step 1) is mixed and water are with 1:(0.5-1.0) ratio material moistening 1-2 hour;

3) under 30-45 ℃ of condition, with lysozyme bacterium, cellulase bacterium, lactobacillus acidophilus activation 1-2 hour;

4) to step 2) profit honest material material in add the lysozyme bacterium 0.5-5 part that has activated, cellulase bacterium 0.5-3 part, lactobacillus acidophilus 0.5-2 part mixes rear beginning heap fermentation;

5) stirring, 25-55 ℃ of temperature controlled fermentation, the stirring time interval is 6-36 hour;

6) anaerobic fermentation 7-15 days, when fermentate pH is 4.0-5.0, oven dry → pulverizing → check → packing → finished product then.

The present invention carries out the specific aim transformation according to the different quality characteristics of two kinds of raw materials, more organically in conjunction with learning from other's strong points to offset one's weaknesses:

Although its crude protein content of blood meal reaches more than 80%, its crude protein digestibility is only 16%, and the albumen absorptivity is too low.Therefore the present invention is first by-acetylmuramic acid and the β between NAG-1 in antalzyme fermentation enzymolysis destroying blood-cell cell membrane, 4 glycosidic bonds, make the insoluble glutinous polysaccharide of cell membrane resolve into the solubility glycopeptide, cause the cell wall rupture content to be overflowed, thereby discharge the mycoprotein of effective nutritional labeling and restructuring; Again with the palm kernel meal with fine amino acid balance under the lactobacillus acidophilus effect, it is better that degree of depth fermenting enzyme solution obtains amino acid balance, the high and little peptide content with fine palatability of digestibility is at the peptide blood meal more than 70%.

The existing greatest problem of palm kernel meal is that crude fiber content is high.Therefore the present invention first by cellulase with the cellulose degradation in palm kernel meal, then the cellobiose after degrading, cellotriose and other low molecule cellodextrin are decomposed into glucose; Again under the lactobacillus acidophilus effect, degree of depth fermenting enzyme solution obtains the high and little peptide content with fine palatability of digestibility at the peptide palm kernel meal more than 70%.

Therefore, the high Toplink bioprotein that is comprised of peptide blood meal, peptide palm kernel meal has high-lysine content, high-octane outstanding advantages, and its ferment content is high, the high and good palatability of digestibility.

High Toplink bioprotein uses as the high bioactivity protein raw material in complete feed, consumption 5-20%,

Have following functions:

1, little peptide content is high, can directly absorb by intestinal mucosa, and transport speed is fast, absorption rate is fast, it is saturated to be difficult for, do not exist and the Amino Acid Absorption competition, can also promote the transhipment of free amino acid, it is low that little peptide absorbs power consumption, improve protein digestibility, improve the price of deed.

2, ANFs is fully purified, and solves the trophism diarrhea of animal.

3, organic acid content is high and contain multiple digestive ferment, can effectively improve animal to the digestion power of feed, uses this patent product needn't add separately fodder acidulant, enzyme preparation, reduces formulation cost.

4, promote growth, reduce feedstuff-meat ratio, obvious increase is laid eggs, the output of milk, shortens feeding time, obviously improves breeding potential, improves meat, egg, milk quality comprehensively.

5, strengthen immunity, reduce disease, greatly save medical fee, improve simultaneously resisting stress capability, reduce the production loss that causes because turning group, vaccine inoculation, temperature drastic change etc.

Good effect of the present invention is:The high Toplink biological protein feedstuff that blood meal after peptide, palm kernel meal reasonable compatibility are made, contain high Toplink bioprotein, for being rich in the probiotics of higher primary school's peptide content, high-energy, high protein, its quality and feeding effect and fermented bean dregs are very approaching, even slightly strengthen.Not only effectively alleviated deficient this global problem of protein, simultaneously because of its low production cost, and the probiotic effect is remarkable, very easily promotes rapidly in vast Feed Enterprise, accelerates China's probiotic and progressively replaces antibiotic process.

Description of drawings

Fig. 1 is SDS-PAGE:(12% separation gel of the present invention) electrophoresis pattern;

The specific embodiment

Embodiment 1:

The 600kg blood meal is mixed with the 400kg palm kernel meal, with 40 ℃, the water material moistening of 600kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 30kg, cellulase bacterium 20kg, the lactobacillus acidophilus 15kg of 1 hour, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 10 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Embodiment 2:

The 600kg blood meal is mixed with the 400kg palm kernel meal, with 35 ℃, the water material moistening of 500kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 20kg, cellulase bacterium 15kg, the lactobacillus acidophilus 10kg of 2 hours, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 7 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Embodiment 3:

The 500kg blood meal is mixed with the 500kg palm kernel meal, with 40 ℃, the water material moistening of 600kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 30kg, cellulase bacterium 20kg, the lactobacillus acidophilus 15kg of 1 hour, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 10 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Embodiment 4:

The 500kg blood meal is mixed with the 500kg palm kernel meal, with 35 ℃, the water material moistening of 500kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 20kg, cellulase bacterium 15kg, the lactobacillus acidophilus 10kg of 2 hours, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 7 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Embodiment 5:

The 800kg blood meal is mixed with the 200kg palm kernel meal, with 40 ℃, the water material moistening of 600kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 30kg, cellulase bacterium 20kg, the lactobacillus acidophilus 15kg of 1 hour, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 10 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Embodiment 6:

The 800kg blood meal is mixed with the 200kg palm kernel meal, with 35 ℃, the water material moistening of 500kg 1 hour, add the water of 35 ℃ to activate lysozyme bacterium 20kg, cellulase bacterium 15kg, the lactobacillus acidophilus 10kg of 2 hours, mix rear beginning heap fermentation, every 6-36 hour stirring once, anaerobic fermentation 7 days, fermentation ends when fermentate pH is 4.0-5.0, then oven dry → pulverizing → check → packing → finished product.

Test example 1

By the high Toplink biological protein feedstuff with embodiment 1～6 preparation, detect through Jilin Province's product quality supervision and testing institute, its technical indicator is as follows:

Crude protein % 〉=45; Small-molecular peptides % 〉=70; Probio cfu/g 〉=1.0 * 10 ¹¹Chicken metabolizable energy kcal/kg:2595 pig metabolizable energy kcal/kg:3585;

High Toplink bioprotein vitamin content unit: mg/100g

Interventions Requested	Assay	Interventions Requested	Assay	Interventions Requested	Assay
						Vitamin A	8.6	Vitamin E	0.36	Cobastab 1	0.66
Cobastab 2	0.14	Vitamin V c	0.48	Vitamin V pp	3.7
						Vitamin V D	1.10	?	?	?	?

Embodiment 1,2 is the high Toplink bioprotein amino acid content unit of protein content 50%: g/100g

Interventions Requested	Assay	Interventions Requested	Assay	Interventions Requested	Assay
						Asparatate	5.5531	Valine	2.2806	Lysine	3.0478
Threonine	2.0525	Methionine	0.6976	Histidine	1.2418
						Serine	2.4757	Isoleucine	2.2137	Arginine	3.3826
Glutamic acid	9.7012	Leucine	3.7718	Proline	2.0562
						Glycine	2.1716	Tyrosine	1.2037	Tryptophan	0.5603
Alanine	2.1806	Phenylalanine	2.4610	Total amino acid content	47.0518

Embodiment 3,4 is the high Toplink bioprotein amino acid content unit of protein content 45%: g/100g

Interventions Requested	Assay	Interventions Requested	Assay	Interventions Requested	Assay
						Aspartic acid	3.3660	Cystine	0.3705	Phenylalanine	2.3886
Threonine	1.7457	Valine	2.7259	Lysine	1.5537
						Serine	2.5819	Methionine	0.6543	Histidine	1.1689
Glutamic acid	9.4034	Isoleucine	1.9650	Arginine	5.1531
						Glycine	1.8962	Leucine	2.8624	Proline	1.7553
Alanine	1.9522	Tyrosine	0.6589	Total amino acid content	41.83

Embodiment 5,6 is the high Toplink bioprotein amino acid content unit of protein content 60%: g/100g

Interventions Requested	Assay	Interventions Requested	Assay	Interventions Requested	Assay
						Aspartic acid	5.77	Cystine	0.47	Phenylalanine	2.24
Threonine	5.52	Valine	3.30	Lysine	4.15
						Serine	1.54	Methionine	0.98	Histidine	1.16
Glutamic acid	7.50	Isoleucine	2.64	Arginine	2.80
						Alanine	2.90	Tyrosine	1.77	Total amino acid content	53.08

Conclusion: detect index by above test and prove absolutely that the crude protein content of the high Toplink biological protein feedstuff of the present invention, little peptide content, beneficial bacterium content, amino acid content, these several most important indexs of identification of organism feed of metabolizable energy value are all higher than fermented bean dregs.

Fig. 1 is degraded to content and the distribution of useful little peptide for the rear protein of fermentation, wherein A is soybean protein, and B is standard items, and C.D is high Toplink bioprotein, and E.F.G is domestic with protein content fermented bean dregs albumen.As shown in the figure, the contained a large amount of antigen proteins of soybean protein, and in high Toplink bioprotein, high molecular weight protein is degraded substantially.

Claims (2)

1. compound high Toplink biological protein feedstuff is characterized in that mainly by following raw materials by weight portion than making:

Blood meal 50-80, palm kernel meal 20-50, lysozyme bacterium 0.5-5, cellulase bacterium 0.5-3, lactobacillus acidophilus 0.5-2.

2. the production technology of compound high Toplink biological protein feedstuff according to claim 1, it comprises the following steps:

1) blood meal is mixed with palm kernel meal;

2) under 30-45 ℃ of condition, the material that step 1) is mixed and water were with the ratio material moistening of 1:0.5-1.0 1-2 hour;

3) under 30-45 ℃ of condition, with lysozyme bacterium, cellulase bacterium, lactobacillus acidophilus activation 1-2 hour;

4) to step 2) profit honest material material in add the lysozyme bacterium 0.5-5 part that has activated, cellulase bacterium 0.5-3 part, lactobacillus acidophilus 0.5-2 part mixes rear beginning heap fermentation;

5) stirring, 25-55 ℃ of temperature controlled fermentation, the stirring time interval is 6-36 hour;

6) anaerobic fermentation 7-15 days, when fermentate pH is 4.0-5.0, oven dry → pulverizing → check → packing → finished product then.

Images