CN102618443A - Microcarrier cell reactor and ventilating pipe thereof - Google Patents
Microcarrier cell reactor and ventilating pipe thereof Download PDFInfo
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- CN102618443A CN102618443A CN2012100828658A CN201210082865A CN102618443A CN 102618443 A CN102618443 A CN 102618443A CN 2012100828658 A CN2012100828658 A CN 2012100828658A CN 201210082865 A CN201210082865 A CN 201210082865A CN 102618443 A CN102618443 A CN 102618443A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention discloses a ventilating pipe for a microcarrier cell reactor, comprising a central ventilating pipe (1), wherein the bottom end of the central ventilating pipe (1) is provided with an air outlet hole (11); the central ventilating pipe (1) is externally provided with a sleeve (2); the sleeve (2) comprises a ventilating network pipe (21) and a defoaming network pipe (23) arranged above the ventilating network pipe (21); a connecting pipe (22) is arranged between the ventilating network pipe (21) and the defoaming network pipe (23); and the cross-section area of each mesh of the ventilating network pipe (21) is smaller than that of each microcarrier in the microcarrier cell reactor, and is smaller than that of the air outlet hole (11). The ventilating pipe disclosed by the invention has the advantages that not only can the oxygen supply need of cell culture be met, but also the damage caused to cells in the ventilating process can be avoided, and the survival rate of cell culture is increased. In addition, the invention also provides the microcarrier cell reactor comprising the ventilating pipe, and the microcarrier cell reactor has the same effect.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of ventpipe that is used for the microcarrier cell reactor.In addition, the invention still further relates to a kind of microcarrier cell reactor that comprises above-mentioned ventpipe.
Background technology
Mammiferous cell cultures refers under the cells in vitro condition, keeps cell growth and the technology of breeding with nutrient solution.The microcarrier cell reactor generally includes culture tank and ventpipe.Hold nutrient solution and cell in the culture tank, for cell provides growing environment, ventpipe is used for to the culture tank delivering gas, and cell is drawn nutrition and the dissolved oxygen in the gas in the nutrient solution in culturing process, for the needs of self growth.
The method that present zooblast microcarrier is cultivated ventilation mainly contains two kinds.A kind of is that the mode that adopts the top layer oxygen supply to add stirring is carried out, and the shortcoming of this method is: the vapour-liquid ratio surface-area is too little, and top layer ventilation oxygen-supplying often oxygen-supplying amount is not enough.Another kind method is to adopt deep ventilation; Air feed is carried out in the bottom of promptly adopting a ventpipe to insert culture tank; The oxygen-supplying amount of this method can satisfy the needs of cell growth, but owing to can catch cell in the process that bubble rises, therefore when bubble breaks in uphill process; The shearing force that produces of breaking can cause necrocytosis with the cell drawing crack, can cause a large amount of cell of damage in whole culture cycle like this.
In view of this; Demand urgently carrying out further optimization design to the ventpipe of microcarrier cell reactor of the prior art; Make its oxygen supply demand that can satisfy cell cultures, can avoid again that pair cell damages in venting process, improve the survival rate of cell cultures.
Summary of the invention
The technical problem that the present invention will solve is that a kind of ventpipe that is used for the microcarrier cell reactor is provided, and makes its oxygen supply demand that can satisfy cell cultures, can avoid again that pair cell damages in venting process, improves the survival rate of cell cultures.In addition, the present invention's another technical problem that will solve is that a kind of microcarrier cell reactor that comprises above-mentioned ventpipe is provided.
For solving the problems of the technologies described above, the present invention provides a kind of ventpipe that is used for the microcarrier cell reactor, comprises central aeration pipe, and the bottom of said central aeration pipe is provided with production well; Said central aeration pipe outside also is provided with sleeve pipe, and said sleeve pipe comprises ventilating net tube and the froth breaking webmaster of being located at said ventilating net tube top, is provided with pipe connecting between said ventilating net tube and the said froth breaking webmaster; The cross-sectional area of the mesh of said ventilating net tube is less than the cross-sectional area of the microcarrier in the said microcarrier cell reactor, and less than the cross-sectional area of said production well.
Preferably, the cross-sectional area of the mesh of said froth breaking webmaster is also less than the cross-sectional area of said production well.
Preferably, said ventilating net tube comprises internal layer webmaster, middle layer webmaster and outer webmaster.
Preferably, said middle layer webmaster and said internal layer webmaster, said outer webmaster three are welded in one.
Preferably, said central aeration pipe top is provided with vacuum breaker, and said vacuum breaker opening direction is a direction from top to bottom.
Preferably, the number of said production well is a plurality of, and the diameter range of said production well is 1mm to 2mm.
Preferably, said froth breaking webmaster and said ventilating net tube are the stainless steel webmaster.
The present invention provides a kind of ventpipe that is used for the microcarrier cell reactor, and its central aeration pipe outside also is provided with sleeve pipe, and sleeve pipe comprises ventilating net tube and the froth breaking webmaster of being located at the ventilating net tube top, is provided with pipe connecting between ventilating net tube and the froth breaking webmaster; The cross-sectional area of the mesh of ventilating net tube is less than the microcarrier cross-sectional area in the microcarrier cell reactor, and less than the cross-sectional area of production well.
Adopt this structure; In the course of the work, insert ventpipe in the culture tank after, make the liquid level of the nutrient solution in the culture tank be higher than ventilating net tube and be lower than the froth breaking webmaster; The liquid level that is nutrient solution is in the pipe connecting place, and nutrient solution can flow in the ventilating net tube through the mesh of ventilating net tube in the culture tank.Feed gas to the central aeration pipe top, gas forms the successive bubble and in ventilating net tube, rises from the bottom ejection of central aeration pipe, and bubble constantly dissolves in the nutrient solution in the process that rises.Because bubble rises, make the inside and outside pressure difference that produces of ventilating net tube, the ventilating net tube bottom, the pressure in the webmaster forms underbalance effect less than outside the webmaster.Under this differential pressure action; The nutrient solution that is positioned at the ventilating net tube bottom outside in the culture tank passes the mesh entering ventilating net tube of ventilating net tube bottom; Meanwhile; The nutrient solution that is rich in gaseous constituent then passes mesh from the top of ventilating net tube, in the miscible nutrient solution outside ventilating net tube, absorbs for cell.
Can find out adopt the ventpipe of this spline structure the to form working cycle of " nutrient solution absorbs gas nutrient-nutrient solution from ventilating net tube Lower Half inflow-nutrient solution and flows out from the ventilating net tube first half in ventilating net tube " by above-mentioned working process.In this working cycle, because the mesh cross-sectional area of ventilating net tube is less than the cross-sectional area of microcarrier used in the said microcarrier cell reactor, and less than the cross-sectional area of production well.Therefore the ventilating net tube microcarrier that attaching cell outward can not get in the ventilating net tube; And can be outside ventilating net tube from the bubble of production well ejection yet; Be that cell and bubble are kept apart by ventilating net tube; The shearing force that has produced when therefore having avoided bubble to break is torn the possibility of killing with cell, has guaranteed the stability of cell cultivation process, has improved the survival rate of cell cultures greatly.
The present invention also provides a kind of microcarrier cell reactor, comprises culture tank; Also comprise aforesaid ventpipe, said ventpipe is plugged in the said culture tank.
Because above-mentioned ventpipe has above-mentioned technique effect, therefore, comprises that the microcarrier cell reactor of this ventpipe also should have identical technique effect, repeats no more at this.
Description of drawings
Fig. 1 is the structural representation of a kind of embodiment of ventpipe provided by the present invention;
Fig. 2 is the partial enlarged drawing of the tube wall of the ventilating net tube among Fig. 1.
Wherein, Reference numeral among Fig. 1 to Fig. 2 and the corresponding relation between the component names are:
Central aeration pipe 1; Sleeve pipe 2; Ventilating net tube 21; Pipe connecting 22; Froth breaking webmaster 23; Internal layer webmaster 211; Middle layer webmaster 212; Outer webmaster 213; Production well 11.
Embodiment
Core of the present invention is that a kind of ventpipe that is used for the microcarrier cell reactor is provided, and makes its oxygen supply demand that can satisfy cell cultures, can avoid again that pair cell damages in venting process, improves the survival rate of cell cultures.In addition, another core of the present invention is that a kind of microcarrier cell reactor that comprises above-mentioned ventpipe is provided.
In order to make those skilled in the art understand technical scheme of the present invention better, the present invention is done further detailed description below in conjunction with accompanying drawing and specific embodiment.
Please refer to Fig. 1, Fig. 1 is the structural representation of a kind of embodiment of ventpipe provided by the present invention.
In a kind of embodiment; The present invention provides a kind of ventpipe that is used for the microcarrier cell reactor; This ventpipe comprises central aeration pipe 1 and the sleeve pipe 2 of being located at central aeration pipe 1 outside; The bottom of central aeration pipe 1 is provided with production well 11, and sleeve pipe 2 comprises ventilating net tube 21 and the froth breaking webmaster 23 of being located at ventilating net tube 21 tops, is provided with pipe connecting 22 between ventilating net tube 21 and the froth breaking webmaster 23; The mesh cross-sectional area of ventilating net tube 21 is less than the cross-sectional area of the microcarrier in the microcarrier cell reactor, and less than the cross-sectional area of production well 11.
Adopt this structure; In the course of the work; After inserting ventpipe in the culture tank; Make the liquid level of the nutrient solution in the culture tank be higher than ventilating net tube 21 and be lower than froth breaking webmaster 23, after promptly the liquid level of nutrient solution was in pipe connecting 22 places, nutrient solution can flow in the ventilating net tube 21 through the mesh of ventilating net tube 21 in the culture tank.Feed gas to central aeration pipe 1 top, gas forms the successive bubble and in ventilating net tube 21, rises from the bottom ejection of central aeration pipe 1, and bubble constantly dissolves in the nutrient solution in the process that rises.Because bubble rises, make the ventilating net tube 21 inside and outside pressure differences that produce, ventilating net tube 21 bottoms, the pressure in the ventilating net tube 21 forms underbalance effect less than outside the ventilating net tube 21.Under this differential pressure action; The nutrient solution that is positioned at ventilating net tube 21 bottom outside in the culture tank passes the mesh entering ventilating net tube 21 of ventilating net tube 21 bottoms; Meanwhile; The nutrient solution that is rich in gaseous constituent then passes mesh from the top of ventilating net tube 21, in the miscible nutrient solution outside ventilating net tube 21, absorbs for cell.
Can find out adopt the ventpipe of this structure the to form working cycle of " nutrient solution absorbs gas nutrient-nutrient solution from ventilating net tube 21 Lower Half inflow-nutrient solutions and flows out from ventilating net tube 21 first halves in ventilating net tube 21 " by above-mentioned working process.In this working cycle; Because the mesh cross-sectional area of ventilating net tube 21 is less than the cross-sectional area of the microcarrier in the microcarrier cell reactor; And less than the cross-sectional area of production well 11, so ventilating net tube 21 trapped airs can not run out of outside the ventilating net tube 21, and the ventilating net tube 21 outer microcarriers that attaching cell can not get in the ventilating net tube 21 yet; Be that cell and bubble are kept apart by ventilating net tube 21; The shearing force that has produced when therefore having avoided bubble to break is torn the possibility of killing with cell, has guaranteed the stability of cell cultivation process, has improved the survival rate of cell cultures greatly.
Other structure formations of above-mentioned ventpipe can also further be set.
In another kind of embodiment, as shown in Figure 1, the cross-sectional area of the mesh of above-mentioned froth breaking webmaster 23 is also less than the cross-sectional area of production well 11.
Adopt this structure formation, in the uphill process of bubble, portion gas mixes and is dissolved in the nutrient solution; It is long-pending that remaining bubble rises to the laggard windrow of nutrient solution liquid level, because the cross-sectional area of the mesh of froth breaking webmaster 23 less than the cross-sectional area of production well 11, makes the bubble of piling up can directly not pass the mesh of froth breaking webmaster 23; But in the cavity of froth breaking webmaster 23, constantly pile up; Mutual extrusion is broken after pressure acquires a certain degree, this moment gas and the liquid separation in the bubble, isolated gas passes the mesh fluid film of froth breaking webmaster 23; Be discharged into the top of culture tank; Isolated liquid then is splashed on the inner tubal wall of froth breaking webmaster 23, and flows downward along inner tubal wall, in the nutrient solution that flows to ventilating net tube 21.
This shows; The froth breaking webmaster 23 of said structure can prevent that bubble from directly passing froth breaking webmaster 23 outsides; Drop down onto nutrient solution liquid level surface after having avoided bubble through the outside; And then the generation of the phenomenons such as pair cell damages of breaking, further guaranteed the job security and the stability of ventpipe.
Please refer to Fig. 2, Fig. 2 is the partial enlarged drawing of the tube wall of the ventilating net tube among Fig. 1.
In the another kind of embodiment, as shown in Figure 2, above-mentioned ventilating net tube 21 can comprise internal layer webmaster 211, middle layer webmaster 212 and outer webmaster 213.Like this, the three-layer network tubular construction has increased the intensity of webmaster, has further strengthened the buffer action of ventilating net tube 21 pair cells and bubble.Particularly, internal layer webmaster 211, middle layer webmaster 212 and outer webmaster 213 threes can adopt the mode of welding to be connected in one, and guarantee the strength and stiffness of ventilating net tube 21, can certainly adopt other modes to connect.
Particularly; Above-mentioned internal layer webmaster 211 can adopt the thin plate of 0.8～1.0mm; The square hole of punching out 10 * 10mm; The mesh of stainless (steel) wire is square on middle layer webmaster 212, the outer webmaster 213, and aperture can be adopted as 300 μ～500 μ, 80 μ～120 μ respectively, can also adopt the mesh of other size and dimensions certainly.
In another kind of embodiment, above-mentioned central aeration pipe 1 top can be provided with vacuum breaker, and the vacuum breaker opening direction is a direction from top to bottom.
Adopt this structure, when ventpipe was inserted in the culture tank, when in central aeration pipe 1, feeding gas, gaseous tension can be opened the valve port of vacuum breaker, made gas continuously feed the culture tank bottom; When stopping in central aeration pipe 1, feeding gas, because the existence of vacuum breaker, the nutrient solution of ventilating net tube 21 bottoms can not flow into the top of central aeration pipe 1 owing to differential pressure action yet through the production well 11 of central aeration pipe 1 bottom.So both guarantee to feed the stability of gas, avoided the liquid refluence again, further strengthened the job security and the stability of ventpipe.
In another kind of embodiment, the above-mentioned number of being located at the production well 11 of central aeration pipe 1 bottom can for example can be specially 6～8 for a plurality of, and the diameter range of production well 11 can be 1mm to 2mm.Like this; After gas imports from central aeration pipe 1 top; The number of bubbles of ejection is more from the production well 11 of central aeration pipe 1 bottom, and the volume of each bubble is less, this make bubble can be more fully in the process that rises with ventilating net tube 21 in nutrient solution contact; So that gas can be dissolved in the nutrient solution better, and then make that effusive nutrient solution offers the more nutrient of cell from ventilating net tube 21 tops.
In another kind of embodiment, froth breaking webmaster 23 can be stainless (steel) wire pipe, for example 316L stainless steel webmaster with ventilating net tube 21.Stainless steel tube not only can guarantee the intensity of ventpipe, and can also avoid the long-term phenomenon that chemical reaction takes place of in nutrient solution, soaking, and has further guaranteed the job stability of ventpipe.Certainly, above-mentioned froth breaking webmaster 23 and ventilating net tube 21 can also adopt the webmaster of the higher material of other stability.
In addition, the present invention also provides a kind of microcarrier cell reactor, comprises culture tank, also comprises ventpipe as above, and ventpipe is plugged in the culture tank.
Because above-mentioned ventpipe has above-mentioned technique effect, therefore, comprises that the microcarrier cell reactor of this ventpipe also should have identical technique effect, repeats no more at this.
More than a kind of microcarrier cell reactor provided by the present invention and ventpipe thereof have been carried out detailed introduction.Used concrete example among this paper principle of the present invention and embodiment are set forth, the explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
Claims (8)
1. a ventpipe that is used for the microcarrier cell reactor comprises central aeration pipe (1), and the bottom of said central aeration pipe (1) is provided with production well (11); It is characterized in that; Said central aeration pipe (1) outside also is provided with sleeve pipe (2); Said sleeve pipe (2) comprises ventilating net tube (21) and is located at the froth breaking webmaster (23) of said ventilating net tube (21) top, is provided with pipe connecting (22) between said ventilating net tube (21) and the said froth breaking webmaster (23); The cross-sectional area of the mesh of said ventilating net tube (21) is less than the cross-sectional area of the microcarrier in the said microcarrier cell reactor, and less than the cross-sectional area of said production well (11).
2. the ventpipe that is used for the microcarrier cell reactor according to claim 1 is characterized in that, the cross-sectional area of the mesh of said froth breaking webmaster (23) is also less than the cross-sectional area of said production well (11).
3. the ventpipe that is used for the microcarrier cell reactor according to claim 2 is characterized in that, said ventilating net tube (21) comprises internal layer webmaster (211), middle layer webmaster (212) and outer webmaster (213).
4. the ventpipe that is used for the microcarrier cell reactor according to claim 3 is characterized in that, said middle layer webmaster (212) is welded in one with said internal layer webmaster (211), said outer webmaster (213) three.
5. according to each described ventpipe that is used for the microcarrier cell reactor of claim 1-4, it is characterized in that said central aeration pipe (1) top is provided with vacuum breaker, said vacuum breaker opening direction is a direction from top to bottom.
6. according to each described ventpipe that is used for the microcarrier cell reactor of claim 1-4, it is characterized in that the number of said production well (11) is a plurality of, the diameter range of said production well (11) is 1mm to 2mm.
7. according to each described ventpipe that is used for the microcarrier cell reactor of claim 1-4, it is characterized in that said froth breaking webmaster (23) and said ventilating net tube (21) are the stainless steel webmaster.
8. a microcarrier cell reactor comprises culture tank; It is characterized in that also comprise each described ventpipe like claim 1-7, said ventpipe is plugged in the said culture tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210082865.8A CN102618443B (en) | 2012-03-26 | 2012-03-26 | Microcarrier cell reactor and ventilating pipe thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210082865.8A CN102618443B (en) | 2012-03-26 | 2012-03-26 | Microcarrier cell reactor and ventilating pipe thereof |
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| Publication Number | Publication Date |
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| CN102618443A true CN102618443A (en) | 2012-08-01 |
| CN102618443B CN102618443B (en) | 2014-09-17 |
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| CN201210082865.8A Active CN102618443B (en) | 2012-03-26 | 2012-03-26 | Microcarrier cell reactor and ventilating pipe thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195044A (en) * | 2014-09-17 | 2014-12-10 | 上海保兴生物设备工程有限公司 | Centrifuging lifting stirrer of bioreactor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101402916A (en) * | 2008-11-18 | 2009-04-08 | 上海日泰医药设备工程有限公司 | Inner hoisting stirrer for cell culture |
| CN101506349A (en) * | 2006-06-08 | 2009-08-12 | 安普罗泰恩公司 | A method to manufacture effective bioreactor |
| CN201678670U (en) * | 2010-05-28 | 2010-12-22 | 南京工业大学 | Stirring oxygen supply bioreactor |
| CN102127505A (en) * | 2010-12-31 | 2011-07-20 | 浙江海洋学院 | Immobilized cell bioreactor |
| CN202543221U (en) * | 2012-03-26 | 2012-11-21 | 上海日泰医药设备工程有限公司 | Microcarrier cell reactor and ventilating pipe thereof |
-
2012
- 2012-03-26 CN CN201210082865.8A patent/CN102618443B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101506349A (en) * | 2006-06-08 | 2009-08-12 | 安普罗泰恩公司 | A method to manufacture effective bioreactor |
| CN101402916A (en) * | 2008-11-18 | 2009-04-08 | 上海日泰医药设备工程有限公司 | Inner hoisting stirrer for cell culture |
| CN201678670U (en) * | 2010-05-28 | 2010-12-22 | 南京工业大学 | Stirring oxygen supply bioreactor |
| CN102127505A (en) * | 2010-12-31 | 2011-07-20 | 浙江海洋学院 | Immobilized cell bioreactor |
| CN202543221U (en) * | 2012-03-26 | 2012-11-21 | 上海日泰医药设备工程有限公司 | Microcarrier cell reactor and ventilating pipe thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195044A (en) * | 2014-09-17 | 2014-12-10 | 上海保兴生物设备工程有限公司 | Centrifuging lifting stirrer of bioreactor |
| CN104195044B (en) * | 2014-09-17 | 2016-10-05 | 上海保兴生物设备工程有限公司 | Bioreactor is centrifuged lift agitator |
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| CN102618443B (en) | 2014-09-17 |
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