CN102612942A - Method for improving salt resistance of turfs of Lolium perenne L. on garbage compost matrices by adopting lanthanum - Google Patents
Method for improving salt resistance of turfs of Lolium perenne L. on garbage compost matrices by adopting lanthanum Download PDFInfo
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Abstract
The invention discloses a method for improving salt resistance of turfs of Lolium perenne L. on garbage compost matrices by adopting lanthanum. The method comprises the following steps of: sowing 0.4g of Lolium perenne L. seeds in 20g of compost, adding 0.2-0.4g.kg<-1> of lanthanum nitrate to a culture medium 13 days after sowing the seeds, and continuing culturing for 14 days; then transplanting turfgrass and matrices in a culture dish onto 280g of saline-alkali soil, wherein the salt contents are respectively 0.5% and 0.8%; and beginning measuring the physiological indexes 30 days after transplanting culture. Experimental results show that harms of salt stress to Lolium perenne L. can be relieved by applying lanthanum; and through lanthanum treatment, the above-ground biomass of Lolium perenne L. can be increased, the content of malondialdehyde, a plasmalemma peroxidation product can be reduced, the relative electric conductivity of leaves can be reduced, protective enzyme activities and proline content can be improved, the osmoregulation function of Lolium perenne L. under salt stress can be enhanced, and harms of free radicals to cells can be lightened, thus harms of salt stress to Lolium perenne L. can be relieved and the stress resistance of Lolium perenne L. can be improved.
Description
Technical field
The invention belongs to environmental protection technical field, relate to the method for cultivating perennial ryegrass with producing fertilizer from refuse in daily life as matrix.A kind of method that adopts lanthanum to improve garbage compost matrix perennial ryegrass turf salt-resistance of saying so more specifically.
Background technology
Producing fertilizer from refuse in daily life contains nutritive elements such as abundant organic matter and nitrogen, phosphorus, potassium, can be used as the turf culture matrix, is used for the urban lawn planting.Producing fertilizer from refuse in daily life is used for the turf culture matrix; Promptly solved the problem that traditional turf production expends rich soil, practiced thrift the agricultural land resource, it is big to alleviate present population pressure; The present situation that land resources is nervous; Widened the application approach of garbage compost again, expense and the garbage compost that reduces garbage disposal such as outwards transports at a difficult problem, also helps the perfect of urban lawn planting system simultaneously.
The lawn is by artificial planting or artificial maintenance management and can provides people to lie fallow, travel and the shape meadow, level ground of appropriate sports.The lawn is as the important component part of urban landscaping, and outstanding and important day by day to the effect that environment for human survival is afforested, beautifies, protects and improved, lawn greening receives people's attention day by day.Research both at home and abroad shows that consumer garbage compost can be used as a kind of fertilizer and improves turfgrass and view and admire characteristic and qualitative character, and the raising of perennial ryegrass growth and quality is had obvious facilitation.Landschoot discovers, compares with blank, and garbage compost can reduce the bulk density of soil, improves soil permeability, and in two growth seasons, can most of required nutritive element be provided for lawn growth at least.Garbage compost and composite fertilizer thereof and chemical fertilizer specific energy mutually obviously promote the growth of stem and the root system of perennial ryegrass to improve biomass, improve the color and luster and the regularity of blade, improve the density and the quality on lawn; Promote the absorption of perennial ryegrass to nitrogen, nitrogen and chlorophyll content are handled with blank apparently higher than chemical fertilizer in the blade.But it is pointed out that compost also has contains a large amount of pollutants, also can cause adverse effect to the growth of lawn plant, therefore, adds allogenic material, and it is significant to improve this proterties of compost.
The production of China's turf at present exists problems, can't satisfy the needs of urban lawn planting, and wherein the development of compounded turf matrix and under-supply be exactly an important limiting factor.In turf was produced, turf production process of every completion will be scalped the above mellow soil of 2 cm at least, 3～4 times continuous operations, and fertile farmland is promptly destroyed.Want recovery table clay fertilizer power, short then need several years, at most decades, go up century-old.For this reason, the new artificial lawn matrix of development has stronger practical significance to replace fertile table soil.
In the urban lawn planting, various adverse environmental factors coerce cultivation and the turf establishment that (drought stress, salt stress, acid rain are coerced and low temperature stress etc.) is unfavorable for turf.The lawn water shortage problem exists always, because the artificial lawn root system is more shallow, is not enough in soil, absorb enough moisture, can only depend on the manual watering, especially in the northern China city, will consume a large amount of water curing lawns every year, and a year irrigation quantity is approximately 0.6 ~ 1.0 m
3/ m
2The soil salinization is one of principal element of restriction turfgrass growth, and salt stress almost influences all important life processes of plant, under the salt stress; The metabolic process of plant cell is obstructed; Cause active oxygen accumulation and ion imbalance, plant strain growth is suppressed, and causes cell death when serious.Being on the rise of acid rain pollution more and more gets more and more people's extensive concerning, and become one of international great ecological environment problem in the present age, and natural environment is caused very big harm, influences the growth of turfgrass.In addition, north China winter is cold, and the turfgrass difficulty of surviving the winter often receives the cold current invasion and attack again and causes phenomenons such as general spot, undergrowth at spring and autumn, influence the attractive in appearance of lawn, the value on reduction lawn.
At present, global saline land area has reached 9.5 hundred million hm
2, Chinese saline land about 2.0 * 10
8Hm
2, accounting for 10% of total area under cultivation, the salinization of soil has become one of important environmental problem.And along with fertilizer amount increases, soil secondary salinization is also more and more heavier, and salt stress almost influences all important life processes of plant.Under the salt stress, the metabolic process of plant cell is obstructed, and causes active oxygen accumulation and ion imbalance, and plant strain growth is suppressed, and can cause cell death when serious.At present existing researchs about the turfgrass salt-resistance mainly concentrate on and set forth anti-salt mechanism, but also do not appear in the newspapers about the research that rare earth is applied to the anti-salt of turfgrass aspect.
Summary of the invention
The present invention is that matrix is cultivated perennial ryegrass with the garbage compost; Applying lanthanum nitrate after a period of time; Turf is transplanted on the saline-alkali soil of different salt content; Through observing the turfgrass growing state and measuring degeneration-resistant physical signs, inquire into the influence of Rare Earth Lanthanum to composting substrate perennial ryegrass under the salt stress, for optimize garbage compost matrix on turf establishment application and improve its salt-resistance theoretical foundation be provided.
For realizing above-mentioned purpose, the present invention provides following technical scheme:
A kind of method that adopts lanthanum to improve garbage compost matrix perennial ryegrass turf salt-resistance is characterized in that being undertaken by following step:
(1) compost is selected from the garbage compost after garbage compost factory ferments;
(2) in culture dish, add above-mentioned compost 20 g, add ryegrass seed 0.4g, be sowed in the culture dish, in medium, added 0.2-0.4gkg in after planting 13 days
-1Lanthanum nitrate; Adding the lanthanum nitrate continued cultivated 14 days;
(3) then with turfgrass stubble height 5cm, the turfgrass in the culture dish is transplanted on the 280g saline-alkali soil together with matrix, saline and alkaline salt content is respectively 0.5%, 0.8%; Laboratory temperature is 25 ~ 32 ℃ between culture period, and relative moisture is 40 ~ 66%;
(4) transplant cultivation and begin to measure physical signs after 30 days.Lawn plant wherein is a perennial ryegrass.
Wherein the saline-alkali soil pH of two kinds of salt content is respectively 7.84,7.93, and unit weight is respectively 1.04,1.20 (g/ml), and electrical conductivity is respectively 1.49x10
3, 1.99x10
3(μ S/cm); Water-holding capacity is respectively 1.04,1.20 (ml/g); Total porosity is respectively 64.52,66.67 (%)
The present invention further discloses described method and increase the application aspect its resistance alleviating the injury of salt stress perennial ryegrass.0.2-0.4gkg particularly
-1The application of lanthanum nitrate aspect the salt-resistance of alleviating salt side of body garbage compost matrix lawn plant turf.
The more detailed preparation method of the present invention is following:
1 material and method
1.1 experiment material
This experiment select for use turfgrass be perennial ryegrass (
Lolium perenneL.), available from Beijing whole world Grenidea Technologies PTE Ltd..Rare earth element is lanthanum nitrate (La (NO
3)
36H
2O).Garbage compost is from Tianjin Xiao Dian garbage compost factory, and the rubbish heap source of manure provides the solid waste that produces in the service activity for the city daily life, also comprises recyclable rubbish, Harmful Waste and other are like heating coal ash the like waste.Compost preparation with handle German technology and the equipment (Zhao Shulan etc., 2011) of adopting, compost preceding with the plastics in the domestic waste, metal, sheet of glassine paper etc. are recyclable utilize material to carry out sorting after, remaining material is as windrow.Composting process is an aerobic fermentation, and the windrow moisture content is 45% ~ 60%, is 6.5-7.5 at pH, and temperature is under 55 ℃ of conditions of 45-, the ventilation oxygen supply, and 30 d ferment.Garbage compost after the fermentation will screen before utilization with foreign material such as further removal all types of plastic, metal, cullet and brick and tile stones, then the garbage compost background is analyzed.The garbage compost main physical and chemical is: organic matter 12.12%, and pH 7.62, saturation moisture content 0.76 mL/g, unit weight 0.85g/mL, full nitrogen 5.18%; Available phosphorus 77.92 mg/kg, full potassium 1.21%, Ca content 30.62 g/kg, Fe19.95 g/kg; Mg 5.78 g/kg, Cu 546.15 mg/kg, Zn 534.53 mg/kg, Pb 163.62 mg/kg; Cr 89.87 mg/kg, Ni 76.26 mg/kg, Cd 2.06 mg/kg, Mn 324.59 mg/kg.
Saline-alkali soil is taken from seashore, Huanghua City, Hebei province, and the saline-alkali soil salt content is respectively 0.5%, 0.8%.The saline-alkali soil pH of two kinds of salt content is respectively 7.84,7.93, and unit weight is respectively 1.04,1.20 (g/ml), and electrical conductivity is respectively 1.49x10
3, 1.99x10
3(μ S/cm); Water-holding capacity is respectively 1.04,1.20 (ml/g); Total porosity is respectively 64.52,66.67 (%).
1.2 experimental technique
In the culture dish of diameter 5.7 cm, add compost 20 g.Select the ryegrass seed 0.4g of full seed, be sowed at respectively in each culture dish.In medium, added the lanthanum nitrate of variable concentrations level in after planting 13 days.Lanthanum nitrate is established 3 concentration levels, is 0,0.2,0.4gkg
-1Adding the lanthanum nitrate continued cultivated 14 days; Then with turfgrass stubble height 5cm; Turfgrass in the culture dish is transplanted on the saline-alkali soil together with matrix, and saline-alkali soil is loaded in the plastic cup of diameter 7.2cm, high 10.5cm, every glass of 280g; Saline-alkali soil is established 2 salinity gradients, and salt content is respectively 0.5%, 0.8%.Transplant to cultivate after 30 days and begin to measure physical signs, plant height of experimental session measurement in per 10 days.3 repetitions are established in experiment.Laboratory temperature is 25 ~ 32 ℃ between culture period, and relative moisture is 40 ~ 66%.
1.3 index determining
(1) mensuration of biomass
The neat root of lawn plant is cradled, and acrial part is clean with distilled water flushing, and blotting paper blots surface moisture, puts into baking oven then, and 1 h that under 105 ℃, completes is dried to constant weight for 80 ℃, measures the dry weight of its acrial part.
(2) mensuration of protective enzyme
The method for distilling of crude enzyme liquid: take by weighing blade 0.2g, the phosphate buffer that adds pH7.8 carries out ice bath and grinds, and extracts homogenate to centrifuge tube, is settled to 10ml, 4 ℃ of following centrifugal (10000r/min) 20 min, and supernatant is the crude extract of enzyme, 4 ℃ of preservations are subsequent use.
The mensuration that superoxide dismutase (SOD) is active: adopt NBT photoreduction met hod (Zhang Zhiliang etc., 2003).(comprise 14.5mmol/L dl-methionine, 3 filling the 3ml reaction mixture
μMol/L EDTA, 2.25mmol/L NBT, 60
μThe mol/L riboflavin solution) in the test tube, adds 50
μThe crude enzyme liquid of L; Be placed on after the mixing on the transparent rack for test tube, accurate irradiation reacts 20 min under 4000 Lx fluorescent lamps in illumination box, is the illumination contrast with the irradiation pipe of enzyme-added liquid not; Simultaneously the blank pipe is placed dark place (replacing enzyme liquid with buffer solution), be used as zeroing.Under the 560nm wavelength, measure light absorption value.SOD active unit is to be a unit of enzyme activity to suppress 50% of NBT photochemical reduction.
The mensuration that peroxidase (POD) is active: the reaction mixture that employing guaiacol method (Zhang Zhiliang etc., 2003) is got 3mL is in cuvette, and contrast replaces with the pH=7.8 phosphate buffer.Add enzyme liquid 100
μL opens manual time-keeping immediately, under 470 nm wavelength, measures light absorption value, whenever at a distance from 0.5 min reading once.With per minute △ A
470Changing 0.01 is a peroxidase activity unit.
The mensuration that catalase (CAT) is active: adopt ultraviolet spectrophotometry (Maria
Et al., 2004).In test tube, add reaction mixture (the phosphate buffer 1.5ml of pH7.8, enzyme liquid 0.2ml, distilled water 1.0ml); Control tube replaces enzyme liquid with buffer solution; In measuring pipe, adding 0.3ml concentration then is the hydrogen peroxide of 0.1mol/L; Timing is immediately simultaneously measured light absorption value, whenever at a distance from 0.5 min reading once under the 240nm wavelength.With per minute △ A
240Changing 0.1 is a catalase activity unit.
(3) mensuration of mda content
Method with reference to (2003) such as Zhang Zhiliangs: get 0.2g lawn plant blade, grind homogenate with 10%TCA and extract, be settled to 10ml, 4000rmin
-1Centrifugal 10min gets supernatant 2ml, reacts 20min in the TBA boiling water bath of adding 2ml 0.6%, and cooling is centrifugal rapidly, gets supernatant and measures light absorption value down respectively at 532nm, 450nm wavelength.Contrast replaces extract with 2ml water.
(4) mensuration of proline content
Method with reference to Li Hesheng (2000).Take by weighing 0.5g lawn plant blade and it is shredded, place Boiling tube respectively, add the sulfosalicylic acid solution of 5ml 3% then to each pipe respectively, in boiling water bath, extract 10min (leaching process will often shake), cooling back 3000 rmin
-1Centrifugal 10min draws the 2ml extract in another clean test tube with ground stopper, adds 2ml glacial acetic acid and 2ml acid ninhydrine, in boiling water, heats 30min, and the cooling back adds 4ml toluene extracts, and vibration 30s leaves standstill a night.With toluene is blank, gets upper strata liquid and under the 520nm wavelength, surveys light absorption value with SHIMADZU product UV-1700 type ultraviolet specrophotometer.
(5) mensuration of relative conductivity
Relative conductivity adopts the conductivity meter method to measure (Tang Zhangcheng, 1999): the deionized water of 10 ml is put into numbered test tube, measure I
0Each sample takes by weighing blade 0.2 g, is cut into 0.5 cm
2Fritter place test tube, accurately add deionized water 10 ml, soak 24 h, use DDS-11D direct-reading conductivity gauge to measure electrical conductivity and be I
1After the mensuration test tube is placed on boiling water bath 0.5 h in the water-bath, kill tissues is surveyed I under room temperature
2Calculate relative conductivity (%)=(I
1-I
0)/(I
2-I
0) * 100%.
1.4 data: data analysis adopts EXCEL and SPSS17.0 analysis software to handle.
2 results and analysis
2.1 lanthanum is to the influence of perennial ryegrass biomass under the salt stress
Under salt stress, decreased growth (acrial part) phenomenon (Yang Fuyu etc., 2001) in various degree can appear in turfgrass.Biomass is the comprehensive embodiment of plant to the salt stress reaction, is the direct indicator (Vicente of plant salt endurance
Et al., 2004).As can beappreciated from fig. 1; The ground biomass of perennial ryegrass is that to be lower than salt content at 0.8% o'clock be 0.5% o'clock in the saline-alkali soil salt content; Explain that the growth that salt stress intensity increases perennial ryegrass has inhibitory action, the ground biomass of perennial ryegrass is all than the increase to some extent that does not add lanthanum, explains that Rare Earth Lanthanum can alleviate the injury of salt stress to perennial ryegrass; Promote the increase of its biomass, and then improve its resistance.For perennial ryegrass, it is close to test set two kinds of la concn treatment effects, all compare according to increasing, but difference is not remarkable.
Subordinate list
Subordinate list
2.2 lanthanum is to the influence of perennial ryegrass plant height under the salt stress
Salt stress is embodied in the upgrowth situation of plant to the influence of plant, can weigh with multiple index, and the length of plant seedlings can be used as one of index of weighing salt resistance of plants.Table 1 and table 2 are that salt stress is handled the influence to the two plant height under the salt stress to the influence and the lanthanum of perennial ryegrass plant height.Can find out that from table July 15, all its plant heights of processing of perennial ryegrass were more or less the same when not applying rare earth and being transplanted on the saline-alkali soil.July 25 after applying Rare Earth Lanthanum, the plant height of perennial ryegrass with do not add comparing of rare earth, increase to some extent, explain that Rare Earth Lanthanum can promote the growth of perennial ryegrass to a certain extent.August 10 added lanthanum and handles with not adding lanthanum and handle after being transplanted on the saline-alkali soil 10, and the plant height of perennial ryegrass all is that the former is greater than the latter; Explanation is after turf is transplanted on the saline-alkali soil; The rare-earth elements of lanthanum that contains in the matrix still works, and has promoted the growth of perennial ryegrass, helps the growth of its root system.Along with the increase of time, the Rare Earth Lanthanum action effect weakens, and adds the lanthanum processing and does not add comparing of lanthanum processing, and plant height slightly increases, but difference is not obvious.This possibly be because in time increase, and Rare Earth Lanthanum is along with the downward seepage of water, and the root of transplanting turf is taken root on saline-alkali soil slowly, causes the action effect decline of rare earth.
Table 1 lanthanum is to the influence of perennial ryegrass plant height under the salt stress
2.3 lanthanum is to the active influence of perennial ryegrass protective enzyme under the salt stress
Salt stress is seen table 2 to the influence and the lanthanum of perennial ryegrass protective enzyme to the influence of perennial ryegrass protective enzyme under the salt stress.Strengthen with salt stress concentration, 3 protective enzyme activity of perennial ryegrass all increase.Add lanthanum and handle than add lanthanum processing raising protective enzyme activity, explain that the lanthanum processing can improve perennial ryegrass is removed unnecessary free radical under salt stress ability, alleviates the injury that salt stress brings.Perennial ryegrass SOD activity is 0.4gkg at the lanthanum consumption
-1The time contrast respectively and increased by 78.7% (
P<0.05) and 40.2%, POD, CAT activity are 0.4gkg at the lanthanum consumption
-1In time, contrasts respectively and increased by 6.6%, 7.4% and 16.8% and 6.1%.
Table 2 lanthanum is to the active influence of perennial ryegrass protective enzyme under the salt stress
2.4 lanthanum is to the influence of perennial ryegrass MDA, proline and relative conductivity under the salt stress
The plant peroxidation of membrane lipids that brings out with active oxygen accumulation that under adverse circumstance, sustains an injury is closely related; MDA is one of snperoxiaized primary product of film fat; The height of film fat peroxidating degree has been reacted in its accumulation, and therefore, people can understand the snperoxiaized degree of film fat through MDA content in the determination test material; And indirect determination film system damage degree (Zhang Yongqing, 2009).
From table 3, can find out with salt stress intensity and strengthen; The perennial ryegrass mda content increases, and explains that salt stress possibly increase the free radical accumulation in the rye cursive script, brings out peroxidation of membrane lipids; Thereby mda content is increased, and therefore influence the growth of perennial ryegrass.Lanthanum is handled and has been reduced the mda content of perennial ryegrass, and wherein the lanthanum consumption is at 0.4gkg
-1The time, respectively comparison according to reduced by 36.2% (
P<0.05) and 13.6%.The perennial ryegrass proline content increases under the salt stress, and lanthanum is handled and do not added lanthanum and handle to compare and improved its proline content.Wherein the lanthanum consumption is at 0.2gkg
-1The time, salt content is that comparison in 0.5% o'clock is according to having increased by 31.4%; In salt content is 0.8% o'clock, and two kinds of concentration treatment effects of lanthanum are identical, all compares according to having increased by 5.4%.Strengthen with salt stress, the relative conductivity of perennial ryegrass also increases, and lanthanum is handled can reduce its relative conductivity, explains that lanthanum has certain stabilization to the cell membrane of perennial ryegrass under the salt stress.The lanthanum consumption is at 0.2gkg
-1The time, the relative conductivity of perennial ryegrass is compared respectively according to having reduced by 12.3% and 13%.
Table 3 lanthanum is to the influence of perennial ryegrass MDA, proline and relative conductivity under the salt stress
3 conclusions
(1) mda content of perennial ryegrass increases with salt stress.Lanthanum is handled and has been reduced its mda content.Proline is that plant is to the responsive strain index of salt alkaline stress reaction.Under the salt marsh condition, no matter be rudimentary plant or higher plant, halophytes is the right and wrong halophytes also, and the accumulation of free proline all takes place.
(2) strengthen with salt stress, proline content increases in the rye cursive script, and lanthanum is handled and improved proline content, explains that lanthanum possibly strengthen the saline-alkaline tolerance of perennial ryegrass through the accumulation proline.The conductive capability of plant cell membrane damage situations and tissue is closely related, and plant moisture content is high more, organizes conductive capability high more.The damage of the plant cell membrane that salt stress and other any environment-stress caused all can make the passing through property increase of cell membrane, to the exchange capacity decline of water and ion, until forfeiture.
(3) strengthen with salt stress intensity, the protective enzyme of perennial ryegrass rises to some extent, applies lanthanum and handles, and has improved three protective enzyme activity, thereby has strengthened the ability of removing free radical.The possible mechanism that Liu builds supposition lanthanum regulation and control antioxidant system such as new is; The one, through complexing film system related functions target position active cell signal transduction system; And as the vigor and the expression that mediate the remote-effects antioxidase; The 2nd, through with the coordination bonding of zymoprotein, the enzyme conformation is changed directly change the activity of enzyme, but concrete mechanism remains further to be inquired into.
In sum, salt stress can suppress the growth of perennial ryegrass, applies lanthanum and can alleviate the injury that salt stress brings.Lanthanum is handled can be through improving the ground biomass of perennial ryegrass; Reduce plasma membrane peroxidating product mda content, reduce the blade relative conductivity, improve protective enzyme activity and proline content; Strengthen the osmotic adjustment function of perennial ryegrass under salt stress; Alleviate the injury of radical pair cell, and then alleviate the injury of salt stress, increase its resistance perennial ryegrass.
Description of drawings
Fig. 1 lanthanum is to the influence of perennial ryegrass ground biomass under the salt stress.
Embodiment
Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Lanthanum nitrate has commercially available, and other used reagent also have commercially available.
Embodiment 1
(1) compost is selected from the garbage compost after garbage compost factory ferments;
(2) in culture dish, add above-mentioned compost 20g, add ryegrass seed 0.4g, be sowed in the culture dish, in medium, added 0.2gkg in after planting 13 days
-1Lanthanum nitrate, continue to cultivate 14 days;
(3) then with turfgrass stubble height 5cm, the turfgrass in the culture dish is transplanted on the 280g saline-alkali soil together with matrix, saline and alkaline salt content is respectively 0.5%, 0.8%; Laboratory temperature is 25 ℃ between culture period, and relative moisture is 45%; Wherein the saline-alkali soil pH of two kinds of salt content is respectively 7.84,7.93, and unit weight is respectively 1.04,1.20 (g/ml), and electrical conductivity is respectively 1.49x10
3, 1.99x10
3(μ S/cm); Water-holding capacity is respectively 1.04,1.20 (ml/g); Total porosity is respectively 64.52,66.67 (%)
(4) transplant cultivation and begin to measure physical signs after 30 days.
Embodiment 2
(1) compost is selected from the garbage compost after garbage compost factory ferments;
(2) in culture dish, add above-mentioned compost 20 g, add ryegrass seed 0.4g, be sowed in the culture dish, in medium, added 0.4gkg in after planting 13 days
-1Lanthanum nitrate, continue to cultivate 14 days;
(3) then with turfgrass stubble height 5cm, the turfgrass in the culture dish is transplanted on the 280g saline-alkali soil together with matrix, saline and alkaline salt content is respectively 0.5%, 0.8%; Laboratory temperature is 30 ℃ between culture period, and relative moisture is 56%;
(4) transplant cultivation and begin to measure physical signs after 30 days.Wherein the saline-alkali soil pH of two kinds of salt content is respectively 7.84,7.93, and unit weight is respectively 1.04,1.20 (g/ml), and electrical conductivity is respectively 1.49x10
3, 1.99x10
3(μ S/cm); Water-holding capacity is respectively 1.04,1.20 (ml/g); Total porosity is respectively 64.52,66.67 (%)
Claims (5)
1. method that adopts lanthanum to improve garbage compost matrix perennial ryegrass turf salt-resistance is characterized in that being undertaken by following step:
(1) compost is selected from the garbage compost after garbage compost factory ferments;
(2) in culture dish, add above-mentioned compost 20 g, add ryegrass seed 0.4g, be sowed in each culture dish, in medium, added 0.2-0.4gkg in after planting 13 days
-1Lanthanum nitrate, continue to cultivate 14 days;
(3) then with turfgrass stubble height 5cm, the turfgrass in the culture dish is transplanted on the 280g saline-alkali soil together with matrix, saline and alkaline salt content is respectively 0.5%, 0.8%; Laboratory temperature is 25 ~ 32 ℃ between culture period, and relative moisture is 40 ~ 66%;
(4) transplant cultivation and begin to measure physical signs after 30 days.
2. the said method of claim 1, wherein the saline-alkali soil pH of two kinds of salt content is respectively 7.84,7.93, and unit weight is respectively 1.04,1.20 (g/ml), and electrical conductivity is respectively 1.49x10
3, 1.99x10
3(μ S/cm); Water-holding capacity is respectively 1.04,1.20 (ml/g); Total porosity is respectively 64.52,66.67 (%)
3. the described method of claim 1 increases the application aspect its resistance alleviating the injury of salt stress to perennial ryegrass.
4. the described application of claim 3, resistance wherein refers to 0.2-0.4gkg
-1Lanthanum nitrate in the salt-resistance of alleviating salt side of body garbage compost matrix perennial ryegrass turf.
5. the described application of claim 3, wherein the lanthanum nitrate consumption is at 0.2gkg
-1
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