CN102608100B - System and method for CARS imaging by using four-wave mixing signals generated by optical fiber - Google Patents
System and method for CARS imaging by using four-wave mixing signals generated by optical fiber Download PDFInfo
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Abstract
The invention relates to a system and a method for CARS imaging by using four-wave mixing signals generated by optical fibers, which comprises a laser light source, a plurality of reflectors, an optical parametric oscillator, a precise displacement table, two long-wave-pass dichroic mirrors, a coupling objective lens, the optical fibers, a two-dimensional scanner, a focusing lens, a supporting sleeve, a sample, a band-pass filter, an imaging lens, a detector and a computer. The light source emits a frequency doubling light beam to pump the optical parametric oscillator and then outputs the light beam, the base frequency light beam emitted by the light source is respectively pumping light and Stokes light of CARS imaging, and four-wave mixing signals excited when the pumping light and the Stokes light are transmitted in optical fibers after the pumping light and the Stokes light are overlapped in time and space are probe light of CARS imaging. They are focused on the sample to produce CARS signal light that does not overlap with their frequencies and can be separated by a long-wavelength-pass dichroic mirror. Compared with the standard CARS system configuration, the invention has no additional devices and has no special requirements on the used devices, thereby simplifying the system and saving the cost.
Description
Technical field
The present invention relates to medical imaging technology and coherent anti-stokes raman scattering imaging technique, especially relate to a kind of method and system of utilizing the four-wave mixing signal that produces in optical fiber to carry out coherent anti-stokes raman scattering imaging.
Background technology
Coherent anti-stokes raman scattering (Coherent anti-Stokes Raman scattering, abbreviation CARS) technology is a kind of novel non-marked molecular resonance imaging technique developing rapidly since 1999, its Raman scattering resonance enhancing mechanism based on chemical bond is carried out imaging, can carry out imaging for the specific chemical analysis in the sample being left intact, comprise C-H, O-P-O, Amide I, the chemical bonds such as H-O (are respectively used to liposome, DNA, protein, detection with hydrate), make it have chemo-selective, also be the specificity of imaging.Due to the coherence of CARS, the high several orders of magnitude of the common Raman scattering signal of its signal intensity ratio, thus under medium excitation light intensity condition, just can obtain the image taking speed of video rate.In addition, CARS also has the feature of high detection sensitivity, sub-micron spatial resolution and three-dimensional imaging.Therefore, CARS technology has been widely used in virus, cell, biological tissue and living small animal imaging, drug delivery process monitoring, and the research field such as pathological changes diagnosis.
CARS microscope consists of bulky ultrafast pulse LASER Light Source and flying-spot microscope conventionally, has hindered it and on human body, has carried out the application of living imaging.Therefore, development by the hand-held of optical fiber transmission light or in peep type CARS imaging technique, become the necessary condition that CARS technology is applied to clinical diagnosis.In CARS imaging process, the frequency overlapping on room and time is ω
ppumping (Pump) light beam and frequency be ω
s(< ω
p) Stokes (Stokes) light beam by strong focusing on sample time, generation beat frequency is ω
p-ω
sstack electromagnetic field.As beat frequency ω
p-ω
sresonant frequency Ω with specific molecular
vibcoupling, meets Ω
vib=ω
p-ω
stime, cause molecular oscillation and give off intensity very high, frequency is ω
as=2 ω
p-ω
santi-Stokes (Anti-Stokes) photon, be also CARS signal photon, between wherein pumping, Stokes and anti-Stokes photon, meet phase-matching condition.Absorbed two pump photons and a Stokes photon, and the anti-Stokes photon inspiring, formed a four-wave mixing (Four-wave mixing is called for short FWM) mechanism, visible CARS is a kind of four-wave mixing optics mechanism that meets phase matching.With other nonlinear optics formation method by optical fiber transmission light, as Two-photon excitation fluorescence imaging produces imaging different (only transmitting single ultrafast pulse light beam in optical fiber) with second harmonic, CARS imaging need to be transmitted pump light and the stokes light overlapping on time and space in same optical fiber, thereby inevitably can in optical fiber, inspire the FWM signal of non phase matching.The frequency of FWM signal photon is ω
fwm=2 ω
p-ω
s, identical with the CARS signal being produced by sample and can not be separated, thus very strong ground unrest can in imaging results, be formed, and its intensity tends to flood sample signal.Therefore, must be suppressed at the FWM signal being produced by optical fiber under normal CARS image-forming condition.
The Chen of University of California and (the M Balu of Potma group, et al.Fiber delivered probe for efficient CARS imaging of tissues.Optics Express, 2010,18 (3): result of study 2380-2388) shows, in single mode fused quartz optical fiber, double-clad photon crystal optical fibre and the big mode field area photonic crystal fiber of measuring at them, all observed very strong FWM signal.In order to eliminate the impact of this signal on imaging results, they have used a long-pass dichroic mirror to carry out filtering FWM signal and only make pump light and stokes light pass through at the output terminal of optical fiber, the CARS signal that their incident sample excitation go out by same long-pass dichroic mirror reflects to side direction, then by an other root multimode fiber, collected, thus CARS signal is separated out from pumping signal.Owing to having used more device, this method is difficult to be used under size-constrained based endoscopic imaging occasion.(the Z Wang of Wong group of Cornell University, et al.Coherent anti-Stokes Raman scattering microscopy imaging with suppression of four-wave mixing in optical fibers.Optics Express, 2011, 19 (9): 7960-7970) proposed a kind of method that changes exciting light polarization state and suppressed FWM signal: before pump light and stokes light are coupled into polarization maintaining optical fibre, first by wave plate, change their polarization state, make the polarization state of the two mutually orthogonal, at this moment they just transmit and can not produce FWM signal in optical fiber, the two is from optical fiber output, with a dual wavelength wave plate, (to pump light, be λ/2 wave plate, to stokes light, be λ wave plate) make the two turn back to again consistent polarization state, thereby after incident sample, can inspire CARS signal, realize the object of using same Optical Fiber Transmission exciting light beam not disturbed by FWM signal with receiving sample CARS signal.
From these methods, can find out, the existing thinking addressing this problem is all to manage to suppress this unfavorable factor of FWM.Do you because FWM signal is stronger, can consider so to utilize it to carry out CARS imaging? the realization that three look CARS imaging techniques are this imagination provides may.In aforesaid CARS imaging process, the molecule absorption in sample two pump photon (ω
p) and a Stokes photon (ω
s), this is double-colored CARS imaging technique.Pump photon (ω of molecule absorption in sample
p), a Stokes photon (ω
s) and a probe photon (ω
pr) after also can produce CARS signal (ω
as=ω
p+ ω
pr-ω
s), such mechanism has formed three look CARS imaging techniques.Owing to needing three kinds of ultrafast pulse light beams that frequency is different, this can greatly increase the cost of LASER Light Source and the difficulty that space-time overlaps and regulates, and therefore three look CARS imaging techniques are used also seldom.If using the FWM signal producing in optical fiber as probe light, it and by pump light and the stokes light of Optical Fiber Transmission, carry out three look CARS imagings, now light source is not proposed to any new requirement, and realized the coincidence on time and space between these three exciting lights.In addition, the pulsed light beam (optical parametric oscillator that substituted volume is huge) inspiring while utilizing high power ultrafast pulse light beam to transmit in optical fiber, be used as the exciting light source of CARS imaging, be the important research direction (relating to the miniaturization of CARS system) of current CARS imaging, this point provides strong theory support for above-mentioned imagination.
Summary of the invention
In order to overcome the deficiency of background technology, the object of this invention is to provide FWM signal that a kind of elimination produces by the Optic transmission fiber method and system to imaging results adverse effect, this adverse effect is the intrinsic problem in optical-fiber type CARS imaging system.The non phase matching FWM signal that the method and system inspire while utilizing pump light and stokes light to transmit in optical fiber is as the probe light of CARS imaging, it has formed three look CARS imaging mechanisms with the pump light by Optical Fiber Transmission together with stokes light, the CARS flashlight now being produced by sample and excitation light frequency are not overlapping, the output terminal (without bulk restriction) that receives signal at optical fiber can be separated CARS signal with a long-pass dichroic mirror, thereby realized the object of using same Optical Fiber Transmission exciting light beam not disturbed by FWM signal with receiving sample CARS signal.
In order to realize described object, one aspect of the present invention is to provide the system that a kind of four-wave mixing (FWM) signal that utilizes optical fiber to produce carries out coherent anti-stokes raman scattering (CARS) imaging, and the technical scheme that described system solution technical matters adopts is:
Comprise LASER Light Source, the first catoptron, the second catoptron, optical parametric oscillator, the 3rd catoptron, the 4th catoptron, the 5th catoptron, the 6th catoptron, the 7th catoptron, the 8th catoptron, precision displacement table, the 9th catoptron, the first long-pass dichroic mirror, the tenth catoptron, coupling object lens, optical fiber, two-dimensional scanner, condenser lens, supporting sleeve, sample, the second long-pass dichroic mirror, band pass filter, imaging len, detector and computing machine, wherein:
The frequency multiplication light beam sending from the output port a of LASER Light Source after the first catoptron and the reflection of the second catoptron, input pump optical parametric oscillator, the light beam of being exported by optical parametric oscillator is the pump light of CARS imaging; The basic frequency beam sending from the output port b of LASER Light Source is the stokes light of CARS imaging, stokes light successively after the 3rd catoptron, the 4th catoptron, the 5th catoptron, the 6th catoptron, the 7th catoptron, the 8th catoptron and the reflection of the 9th catoptron to the first long-pass dichroic mirror, this light path is Stokes light path;
The 6th catoptron and the 7th catoptron in Stokes light path are arranged in precision displacement table; By adjustment precision displacement platform and the 8th catoptron and the 9th catoptron, make respectively in time and space, to overlap by the first long-pass dichroic mirror from the pump light of optical parametric oscillator output and the stokes light that comes from the 9th catoptron; The pump light overlapping on time and space and stokes light, after the tenth catoptron reflexes to the second long-pass dichroic mirror, are coupled in object lens coupled into optical fibres and transmit; When the pump light overlapping on time and space and stokes light transmit in optical fiber, inspire the four-wave mixing signal of non phase matching, the probe light using four-wave mixing signal as CARS imaging; From pump light, stokes light and the probe light of optical fiber output, be focused lens focus on sample and inspire CARS signal; The end of optical fiber is fixed on two-dimensional scanner, and makes optical fiber stretch out a bit of semi-girder that becomes, and two-dimensional scanner and condenser lens are arranged in supporting sleeve;
The CARS flashlight being produced by sample and by the pump light of sample retroreflection or scattering, stokes light and probe light, be focused that lens are collected and coupled into optical fibres in transmit, then after coupling object lens to the second long-pass dichroic mirror; By the pump light of sample retroreflection or scattering, stokes light and probe light, seen through the second long-pass dichroic mirror, CARS flashlight is detected device by the second long-pass dichroic mirror reflects after by band pass filter and imaging len and receives;
The two-dimensional scanner that computer control is arranged in supporting sleeve resonates with optical fiber end, the pump light, stokes light and the probe light that make to be focused lens focus carry out two-dimensional scan on sample, computing machine is controlled detector synchronous acquisition CARS signal simultaneously and is transferred to computing machine and processes, thereby obtains the two-dimentional CARS imaging results of sample.
Described LASER Light Source is the high-frequency impulse near-infrared laser light source with 2～7ps or 102fs magnitude pulse width, and described high-frequency impulse near-infrared laser light source has the basic frequency beam sending from output port b, the frequency multiplication light beam sending from output port a.
Described optical parametric oscillator is that the pulse width with LASER Light Source matches, wavelength is at the adjustable laser generator of near-infrared band.
The light beam of the 5th described catoptron to the six catoptrons all regulates direction parallel with the displacement of precision displacement table with the direction of the light beam of the 7th catoptron to the eight catoptrons.
The input end end face of described optical fiber is positioned on the front focal plane of coupling object lens.
The light-sensitive surface of described detector is positioned on the front focal plane of imaging len.
In order to realize described object, the present invention is to provide a kind of method that four-wave mixing signal that utilizes optical fiber to produce carries out CARS imaging on the other hand, comprises the following steps:
Step S1: the stokes light using the basic frequency beam of LASER Light Source output as CARS imaging, lentor light frequency is designated as ω
s; Pump light using the light beam that produces after the frequency multiplication light beam pump optical parametric oscillator of LASER Light Source output and export as CARS imaging, pumping light frequency is designated as ω
p;
Step S2: by regulating the catoptron in Stokes light path, stokes light and pump light are spatially overlapped after passing through the first long-pass dichroic mirror;
Step S3: by regulating the precision displacement table in Stokes light path, stokes light and pump light are overlapped in time after passing through the first long-pass dichroic mirror;
Step S4: by regulating the temperature of gain media in optical parametric oscillator to carry out the pump light wavelength of regulation output, make the difference on the frequency of pump light and stokes light and the resonant frequency Ω of testing molecule chemical bond
vibmatch, meet: Ω
vib=ω
p-ω
s;
Step S5: the frequency inspiring when the pump light overlapping on time and space and stokes light are transmitted in optical fiber is ω
fwm=2 ω
p-ω
snon phase matching four-wave mixing signal, as the probe light of CARS imaging, probe light frequency is designated as ω
pr;
Step S6: after pump light, stokes light and probe light are focused on sample by condenser lens, inspire the CARS flashlight that meets phase-matching condition, CARS signal light frequency is ω
as=ω
p+ ω
pr-ω
s;
Step S7: the CARS flashlight being produced by sample, and be focused in lens collection coupled into optical fibres and transmit by the pump light of sample retroreflection or scattering, stokes light and probe light, fiber-optic output only have CARS flashlight by the second long-pass dichroic mirror reflects to detector, the light signal that detector receives is converted into and transfers to computing machine after electric signal and process;
Step S8: computer control two-dimensional scanner resonates with optical fiber end, makes focused beam on sample, carry out two-dimensional scan, thereby obtains the two-dimentional CARS imaging results of sample.
Compare with background technology, the beneficial effect that the present invention has is:
1, the present invention has eliminated the FWM signal that produced by Optic transmission fiber in the optical-fiber type CARS imaging system adverse effect to imaging results.The fiberize of CARS system is the necessary condition of its clinical practice, and when optical fiber transmits, inevitably can inspire non phase matching FWM signal for pump light and the stokes light of CARS imaging, the CARS signal that this signal and sample produce has identical frequency and can not be separated, and the strong sample signal that consequently usually floods of its intensity.
2, the present invention has realized and has used same Optical Fiber Transmission exciting light beam and receive sample CARS signal and the object that not disturbed by FWM signal.The existing way addressing this problem is to transmit exciting light beam with an optical fiber, and the FWM signal that optical fiber produces carrys out filtering with a long-pass dichroic mirror, then with another root optical fiber, receives the CARS signal of sample.
3, the newly-increased any device of relative standard CARS system configuration of the present invention, and to the device using without any specific (special) requirements, greatly simplified system and saved cost.Adopt the method that two optical fiber transmit respectively exciting light beam and reception signal beams to increase more device newly, in size-constrained, peep under condition and be difficult to be used; And the method for utilizing dual wavelength wave plate need to be introduced the polarization state that some wave plates and polaroid change incident exciting light beam, and dual wavelength wave plate needs specific customization.
4, the present invention is applicable to the CARS imaging system of any fiber type, for realizing CARS based endoscopic imaging, provides simple and effective solution route.
Accompanying drawing explanation
Fig. 1 is system schematic of the present invention;
Fig. 2 a is the energy level schematic diagram of double-colored CARS imaging;
Fig. 2 b is the energy level schematic diagram of three look CARS imagings;
Fig. 3 is the inventive method process flow diagram.
Symbol description in figure:
1 LASER Light Source, 2 first catoptrons, 3 second catoptrons,
4 optical parametric oscillators, 5 the 3rd catoptrons, 6 the 4th catoptrons,
7 the 5th catoptrons, 8 the 6th catoptrons, 9 the 7th catoptrons,
10 the 8th catoptrons, 11 precision displacement table, 12 the 9th catoptrons,
14 the tenth catoptrons, 15 coupling object lens, 16 optical fiber,
17 two-dimensional scanners, 18 condenser lenses, 19 supporting sleeves,
20 samples, 22 band pass filters, 23 imaging lens,
24 detectors, 25 computing machines,
13 first long-pass dichroic mirrors, 21 second long-pass dichroic mirrors.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated:
As shown in Figure 1, the system that the four-wave mixing signal that utilizes optical fiber to produce that the present invention proposes carries out CARS imaging comprises: LASER Light Source 1, the first catoptron 2, the second catoptron 3, optical parametric oscillator 4, the 3rd catoptron 5, the 4th catoptron 6, the 5th catoptron 7, the 6th catoptron 8, the 7th catoptron 9, the 8th catoptron 10, precision displacement table 11, the 9th catoptron 12, the first long-pass dichroic mirror 13, the tenth catoptron 14, coupling object lens 15, optical fiber 16, two-dimensional scanner 17, condenser lens 18, supporting sleeve 19, sample 20, the second long-pass dichroic mirror 21, band pass filter 22, imaging len 23, detector 24 and computing machine 25.
LASER Light Source 1 is for having 2～7ps or 10
2the high-frequency impulse near-infrared laser light source of fs magnitude pulse width, it has the basic frequency beam sending from output port b, the frequency multiplication light beam sending from output port a.Frequency multiplication light beam is after the first catoptron 2 and the second catoptron 3 reflections, and input is pump optical parametric oscillator 4 also.Optical parametric oscillator 4 be that the pulse width with LASER Light Source 1 matches, wavelength at the adjustable laser generator of near-infrared band, the pump light (ω by the light beam of its output as CARS imaging
p).The basic frequency beam sending from the output port b of LASER Light Source 1 is as the stokes light (ω of CARS imaging
s), successively after the 3rd catoptron 5, the 4th catoptron 6, the 5th catoptron 7, the 6th catoptron 8, the 7th catoptron 9, the 8th catoptron 10 and the 9th catoptron 12 reflections to the first long-pass dichroic mirror 13, this light path has formed Stokes light path.
The 6th catoptron 8 and the 7th catoptron 9 in Stokes light path are arranged in precision displacement table 11.By adjustment precision displacement platform 11 and the 8th catoptron 10 and the 9th catoptron 12, make respectively in time and space, to overlap by the first long-pass dichroic mirror 13 from the pump light of optical parametric oscillator 4 output with from the stokes light of the 9th catoptron 12; The pump light overlapping on time and space and stokes light, after the tenth catoptron 14 reflexes to the second long-pass dichroic mirror 21, are coupled transmission in object lens 15 coupled into optical fibres 16; When the pump light overlapping on time and space and stokes light transmit in optical fiber 16, inspire the four-wave mixing signal of non phase matching, the probe light using four-wave mixing signal as CARS imaging; Pump light, stokes light and the probe light from optical fiber 16, exported are focused lens 18 and focus on sample 20 and inspire CARS signal; The end of optical fiber 16 is fixed on two-dimensional scanner 17, and makes optical fiber 16 stretch out a bit of semi-girder that becomes, and two-dimensional scanner 17 and condenser lens 18 are arranged in supporting sleeve 19;
The pump light that the first long-pass dichroic mirror 13 reflects from optical parametric oscillator 4, and see through the stokes light from the 9th catoptron 12.The 5th catoptron 7 to the light beam of the 6th catoptron 8 regulates direction parallel with the 7th catoptron 9 to the direction of the light beam of the 8th catoptron 10 and the displacement of precision displacement table 11, so that when the time of carrying out pump light and stokes light by precision displacement table 11 overlap to regulate, can guarantee from the direction of the light beam of the 9th catoptron 12 to first long-pass dichroic mirrors 13 constant.
By regulating the 8th catoptron 10 and the 9th catoptron 12 in Stokes light path, pump light and stokes light are spatially overlapped after passing through the first long-pass dichroic mirror 13.Pump light and stokes light, through the tenth catoptron 14 reflections with through after the second long-pass dichroic mirror 21, are coupled transmission in object lens 15 coupled into optical fibres 16, and the input end end face of optical fiber 16 is positioned on the front focal plane of coupling object lens 15.When the pump light overlapping on time and space and stokes light transmit in optical fiber 16, can inspire frequency is ω
fwm=2 ω
p-ω
snon phase matching four-wave mixing signal, the probe light using this signal as CARS imaging, its frequency is designated as ω
pr.
Exciting light from optical fiber 16 outputs, comprises pump light, stokes light and probe light, is focused lens 18 and focuses on sample 20, inspires the CARS signal that meets phase-matching condition, and its frequency is ω
as=ω
p+ ω
pr-ω
s.The end of optical fiber 16 is fixed on two-dimensional scanner 17, and makes optical fiber 16 stretch out a bit of formation cantilever beam structure, and two-dimensional scanner 17 and condenser lens 18 are arranged in supporting sleeve 19.Computing machine 25 is controlled two-dimensional scanner 17 with the bracketed part resonance of optical fiber 16, makes the exciting light beam that is focused lens 18 focusing on sample 20, carry out two-dimensional scan.
The CARS flashlight being produced by sample 20 and by pump light, stokes light and the probe light of sample 20 retroreflections or scattering, be focused that lens 18 are collected and coupled into optical fibres 16 in transmission, then after coupling object lens 15 to the second long-pass dichroic mirror 21.By pump light, stokes light and the probe light of sample 20 retroreflections or scattering, seen through the second long-pass dichroic mirror 21, CARS flashlight is received by being detected device 24 after band pass filter 22 and imaging len 23 by the second long-pass dichroic mirror 21 reflections;
The second long-pass dichroic mirror 21 sees through by pump light, stokes light and the probe light of sample 20 retroreflections or scattering, and reflection CARS flashlight is to feeler arm.In feeler arm, CARS flashlight is imaged after by band pass filter 22 on the light-sensitive surface that lens 23 focus on detector 24, and all kinds of parasitic lights that produce in system are by band pass filter 22 filterings.
Fig. 2 a and Fig. 2 b are respectively double-colored and the energy level schematic diagram of three look CARS imagings.In the double-colored CARS imaging process shown in Fig. 2 a, two pump photons of molecule absorption and a Stokes photon in sample 20, as the difference of frequency and the resonant frequency Ω of testing molecule of the two
vibcoupling, meets Ω
vib=ω
p-ω
stime, causing molecular oscillation and giving off frequency is ω
as=2 ω
p-ω
scARS signal.And in three look CARS imaging processes shown in Fig. 2 b, the pump photon of molecule absorption in sample 20, a Stokes photon and a probe photon, the sample CARS flashlight frequency now inspiring is ω
as=ω
p+ ω
pr-ω
swith exciting light zero lap, the output terminal that can receive signals at optical fiber 16 by the second long-pass dichroic mirror 21 CARS signal extraction out, is used same optical fiber 16 transmission exciting light beams and receives sample CARS signal and be not subject to the object of FWM signal interference thereby realized.
As embodiment, LASER Light Source 1 can adopt the picoTRAIN IC-1064-10000 type laser instrument of Austrian High-Q Laser company, it can provide the fundamental frequency pulse output beam of 76MHz pulsed frequency, 7ps pulse width, 1064nm wavelength, and the double frequency pulse output beam of 532nm wavelength.The Levante Emerald psec product of the German APE GmbH of optical parametric oscillator 4 employing company, the pulsed light beam of 532nm wavelength flows to optical parametric oscillator 4 as pump light, can obtain output beam adjustable, that pulse width is 5ps in 680～990nm wavelength coverage, as the pump light of CARS imaging.The 1064nm wavelength pulse light beam of being exported by LASER Light Source 1 is as the stokes light of CARS imaging.The first long-pass dichroic mirror 13, the second long-pass dichroic mirror 21 and band pass filter 22 adopt respectively Q1020LPXR, 640DCXR and the HQ560/50m type product of U.S. Chroma Technology company, R3896 type photomultiplier (PMT) detector of the Japanese Bin Song of detector 24 employing company.All the other devices, all can buy from market.CH
2chemical bond is often used in the imaging of biosome, and its resonant frequency is 2845cm
-1, now need pump light to be adjusted to 816.8nm (12243cm
-1), stokes light is fixed on 1064nm (9398cm
-1), the FWM signal that produces in optical fiber is that probe light is 662.8nm (15088cm
-1), the CARS signal light wavelength that sample produces is 557.6nm (17933cm
-1).
Fig. 3 illustrates the method that the four-wave mixing signal that utilizes optical fiber to produce that the present invention proposes carries out CARS imaging, comprises the following steps:
Step S1: the stokes light using the basic frequency beam of LASER Light Source output as CARS imaging, lentor light frequency is designated as ω
s; Pump light using the light beam that produces after the frequency multiplication light beam pump optical parametric oscillator of LASER Light Source output and export as CARS imaging, pumping light frequency is designated as ω
p;
Step S2: by regulating the catoptron in Stokes light path, stokes light and pump light are spatially overlapped after passing through the first long-pass dichroic mirror;
Step S3: by regulating the precision displacement table in Stokes light path, stokes light and pump light are overlapped in time after passing through the first long-pass dichroic mirror;
Step S4: by regulating the temperature of gain media in optical parametric oscillator to carry out the pump light wavelength of regulation output, make the difference on the frequency of pump light and stokes light and the resonant frequency Ω of testing molecule chemical bond
vibmatch, meet: Ω
vib=ω
p-ω
s;
Step S5: the frequency inspiring when the pump light overlapping on time and space and stokes light are transmitted in optical fiber is ω
fwm=2 ω
p-ω
snon phase matching four-wave mixing signal, as the probe light of CARS imaging, probe light frequency is designated as ω
pr;
Step S6: after pump light, stokes light and probe light are focused on sample by condenser lens, inspire the CARS flashlight that meets phase-matching condition, CARS signal light frequency is ω
as=ω
p+ ω
pr-ω
s;
Step S7: the CARS flashlight being produced by sample, and be focused in lens collection coupled into optical fibres and transmit by the pump light of sample retroreflection or scattering, stokes light and probe light, fiber-optic output only have CARS flashlight by the second long-pass dichroic mirror reflects to detector, the light signal that detector receives is converted into and transfers to computing machine after electric signal and process;
Step S8: computer control two-dimensional scanner resonates with optical fiber end, makes focused beam on sample, carry out two-dimensional scan, thereby obtains the two-dimentional CARS imaging results of sample.
Above-mentioned embodiment is used for the present invention that explains, rather than limits the invention.In the protection domain of spirit of the present invention and claim, any modification and change that the present invention is made, all fall into protection scope of the present invention.
Claims (7)
1. the system that the four-wave mixing signal that utilizes optical fiber to produce carries out CARS imaging, it is characterized in that: comprise LASER Light Source (1), the first catoptron (2), the second catoptron (3), optical parametric oscillator (4), the 3rd catoptron (5), the 4th catoptron (6), the 5th catoptron (7), the 6th catoptron (8), the 7th catoptron (9), the 8th catoptron (10), precision displacement table (11), the 9th catoptron (12), the first long-pass dichroic mirror (13), the tenth catoptron (14), coupling object lens (15), optical fiber (16), two-dimensional scanner (17), condenser lens (18), supporting sleeve (19), sample (20), the second long-pass dichroic mirror (21), band pass filter (22), imaging len (23), detector (24) and computing machine (25), wherein:
The frequency multiplication light beam sending from the output port a of LASER Light Source (1) is after the first catoptron (2) and the second catoptron (3) reflection, input pump optical parametric oscillator (4), the light beam of being exported by optical parametric oscillator (4) is the pump light of CARS imaging; The basic frequency beam sending from the output port b of LASER Light Source (1) is the stokes light of CARS imaging, stokes light successively after the 3rd catoptron (5), the 4th catoptron (6), the 5th catoptron (7), the 6th catoptron (8), the 7th catoptron (9), the 8th catoptron (10) and the reflection of the 9th catoptron (12) to the first long-pass dichroic mirror (13), this light path is Stokes light path;
The 6th catoptron (8) and the 7th catoptron (9) in Stokes light path are arranged in precision displacement table (11); By adjustment precision displacement platform (11) and the 8th catoptron (10) and the 9th catoptron (12), make respectively in time and space, to overlap by the first long-pass dichroic mirror (13) from the pump light of optical parametric oscillator (4) output and the stokes light that comes from the 9th catoptron (12); The pump light overlapping on time and space and stokes light, after the tenth catoptron (14) reflexes to the second long-pass dichroic mirror (21), are coupled transmission in object lens (15) coupled into optical fibres (16); When the pump light overlapping on time and space and stokes light transmit in optical fiber (16), inspire the four-wave mixing signal of non phase matching, the probe light using four-wave mixing signal as CARS imaging; Pump light, stokes light and the probe light from optical fiber (16), exported are focused lens (18) and focus on sample (20) and inspire CARS signal; It is upper that the end of optical fiber (16) is fixed on two-dimensional scanner (17), and make optical fiber (16) stretch out a bit of semi-girder that becomes, and two-dimensional scanner (17) and condenser lens (18) are arranged in supporting sleeve (19);
By the CARS flashlight of sample (20) generation with by pump light, stokes light and the probe light of sample (20) retroreflection or scattering, be focused that lens (18) are collected and coupled into optical fibres (16) in transmission, then after coupling object lens (15) to the second long-pass dichroic mirror (21); By pump light, stokes light and the probe light of sample (20) retroreflection or scattering, seen through the second long-pass dichroic mirror (21), CARS flashlight is received by being detected device (24) after band pass filter (22) and imaging len (23) by the second long-pass dichroic mirror (21) reflection;
Computing machine (25) is controlled the two-dimensional scanner (17) being arranged in supporting sleeve (19) and is resonated with optical fiber end, make the pump light, stokes light and the probe light that are focused lens (18) focusing carry out two-dimensional scan on sample (20), computing machine (25) is controlled detector (24) synchronous acquisition CARS signal simultaneously and is transferred to computing machine (25) and processes, thereby obtains the two-dimentional CARS imaging results of sample.
2. the system that the four-wave mixing signal that utilizes optical fiber to produce according to claim 1 carries out CARS imaging, it is characterized in that: described LASER Light Source (1) is for having the high-frequency impulse near-infrared laser light source of 2～7ps or 102fs magnitude pulse width, and described high-frequency impulse near-infrared laser light source has the basic frequency beam sending from output port b, the frequency multiplication light beam sending from output port a.
3. the system of utilizing four-wave mixing signal that optical fiber produces to carry out CARS imaging according to claim 1, is characterized in that: described optical parametric oscillator (4) is that the pulse width with LASER Light Source (1) matches, wavelength is at the adjustable laser generator of near-infrared band.
4. the system that the four-wave mixing signal that utilizes optical fiber to produce according to claim 1 carries out CARS imaging, is characterized in that: the 5th described catoptron (7) is all parallel with the displacement adjusting direction of precision displacement table (11) to the direction of the light beam of the 8th catoptron (10) with the 7th catoptron (9) to the light beam of the 6th catoptron (8).
5. the system that the four-wave mixing signal that utilizes optical fiber to produce according to claim 1 carries out CARS imaging, is characterized in that: the input end end face of described optical fiber (16) is positioned on the front focal plane of coupling object lens (15).
6. the system that the four-wave mixing signal that utilizes optical fiber to produce according to claim 1 carries out CARS imaging, is characterized in that: the light-sensitive surface of described detector (24) is positioned on the front focal plane of imaging len (23).
7. right to use requires the method that described in 1, the four-wave mixing signal that utilizes optical fiber to produce of CARS imaging system carries out CARS imaging, comprises the following steps:
Step S1: the stokes light using the basic frequency beam of LASER Light Source output as CARS imaging, lentor light frequency is designated as ω
s; Pump light using the light beam that produces after the frequency multiplication light beam pump optical parametric oscillator of LASER Light Source output and export as CARS imaging, pumping light frequency is designated as ω
p;
Step S2: by regulating the catoptron in Stokes light path, stokes light and pump light are spatially overlapped after passing through the first long-pass dichroic mirror;
Step S3: by regulating the precision displacement table in Stokes light path, stokes light and pump light are overlapped in time after passing through the first long-pass dichroic mirror;
Step S4: by regulating the temperature of gain media in optical parametric oscillator to carry out the pump light wavelength of regulation output, make the difference on the frequency of pump light and stokes light and the resonant frequency Ω of testing molecule chemical bond
vibmatch, meet: Ω
vib=ω
p-ω
s;
Step S5: the frequency inspiring when the pump light overlapping on time and space and stokes light are transmitted in optical fiber is ω
fwm=2 ω
p-ω
snon phase matching four-wave mixing signal, as the probe light of CARS imaging, probe light frequency is designated as ω
pr;
Step S6: after pump light, stokes light and probe light are focused on sample by condenser lens, inspire the CARS flashlight that meets phase-matching condition, CARS signal light frequency is ω
as=ω
p+ ω
pr-ω
s;
Step S7: the CARS flashlight being produced by sample, and be focused in lens collection coupled into optical fibres and transmit by the pump light of sample retroreflection or scattering, stokes light and probe light, fiber-optic output only have CARS flashlight by the second long-pass dichroic mirror reflects to detector, the light signal that detector receives is converted into and transfers to computing machine after electric signal and process;
Step S8: computer control two-dimensional scanner resonates with optical fiber end, makes focused beam on sample, carry out two-dimensional scan, thereby obtains the two-dimentional CARS imaging results of sample.
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Families Citing this family (21)
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| CN115420729B (en) * | 2022-11-02 | 2023-06-23 | 中国科学院新疆理化技术研究所 | Method for enhancing Raman effect based on multi-step excitation 244nm laser |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070088219A1 (en) * | 2005-10-13 | 2007-04-19 | Xie Xiaoliang S | System and method for coherent anti-stokes raman scattering endoscopy |
| US7289203B2 (en) * | 2004-09-30 | 2007-10-30 | Chromaplex, Inc. | Method and system for spectral analysis of biological materials using stimulated cars |
| WO2007124384A1 (en) * | 2006-04-21 | 2007-11-01 | Intel Corporation | Apparatus and method for imaging with surface enhanced coherent anti-stokes raman scattering (secars) |
| US20100020318A1 (en) * | 2008-07-24 | 2010-01-28 | Korea Research Institute Of Standards And Science | 3-Color multiplex cars spectrometer |
| CN102156115A (en) * | 2011-02-25 | 2011-08-17 | 深圳大学 | Coherent anti-Stokes Raman scattering microscopic method and system of super-diffraction limit |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7586618B2 (en) * | 2005-02-28 | 2009-09-08 | The Board Of Trustees Of The University Of Illinois | Distinguishing non-resonant four-wave-mixing noise in coherent stokes and anti-stokes Raman scattering |
-
2012
- 2012-03-02 CN CN201210053798.7A patent/CN102608100B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7289203B2 (en) * | 2004-09-30 | 2007-10-30 | Chromaplex, Inc. | Method and system for spectral analysis of biological materials using stimulated cars |
| US20070088219A1 (en) * | 2005-10-13 | 2007-04-19 | Xie Xiaoliang S | System and method for coherent anti-stokes raman scattering endoscopy |
| WO2007124384A1 (en) * | 2006-04-21 | 2007-11-01 | Intel Corporation | Apparatus and method for imaging with surface enhanced coherent anti-stokes raman scattering (secars) |
| US20100020318A1 (en) * | 2008-07-24 | 2010-01-28 | Korea Research Institute Of Standards And Science | 3-Color multiplex cars spectrometer |
| CN102156115A (en) * | 2011-02-25 | 2011-08-17 | 深圳大学 | Coherent anti-Stokes Raman scattering microscopic method and system of super-diffraction limit |
Non-Patent Citations (7)
| Title |
|---|
| Coherent anti-Stokes Raman scattering microscopy imaging with suppression of four-wave mixing in optical fibers;Zhiyong Wang et，al.;《OPTICS EXPRESS》;20110411;第19卷(第9期);图1及第7963页第1段 * |
| Coherent anti-Stokes Raman scattering microscopy using photonic crystal fiber with two closely lying zero dispersion wavelengths;Sangeeta Murugkar et，al.;《Optical Society of America》;20071017;第15卷(第21期);14028-14037 * |
| Fiber delivered probe for efficient CARS imaging of tissues;Mihaela Balu et，al.;《Opt Express》;20100201;第18卷(第3期);2380-2388 * |
| Four-Wave Mixing in an Optical Fiber in the Zero-Dispersion Wavelength Region;Kyo Inoue;《JOURNAL OF LIGHTWAVE TECHNOLOGY》;19921130;第10卷(第11期);1553-1561 * |
| Multi-Color CARS for Hydrogen-Fueled Scramjet Applications;A. C. Eckbreth et al.;《Appl. Phys.B》;19880430;第45卷(第5期);215-233 * |
| Picosecond anti-Stokes generation in a photonic-crystal fiber for interferometric CARS microscopy;Esben Ravn Andresen et，al.;《Optics Express》;20060807;第14卷(第16期);7246-7251 * |
| Three-color multiplex CARS for fast imaging and microspectroscopy in the entire CHn stretching vibrational region;Jae Yong Lee et al.;《OPTICS EXPRESS》;20091123;第17卷(第25期);22281-22295 * |
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