CN102605037B - Candida selection medium for stool specimens and preparation method thereof - Google Patents
Candida selection medium for stool specimens and preparation method thereof Download PDFInfo
- Publication number
- CN102605037B CN102605037B CN 201210056483 CN201210056483A CN102605037B CN 102605037 B CN102605037 B CN 102605037B CN 201210056483 CN201210056483 CN 201210056483 CN 201210056483 A CN201210056483 A CN 201210056483A CN 102605037 B CN102605037 B CN 102605037B
- Authority
- CN
- China
- Prior art keywords
- candida
- preparation
- substratum
- agar
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a candida selection medium for stool specimens and a preparation method of the candida selection medium. The candida selection medium for the tool specimens is characterized by comprising the following components in each 1000ml culture medium: malt extract 3-10g; beef heart for infusion 10-20g; magnesium sulfate 1.2-2g; agar 15-20g; gentamycin sulfate 0.005-0.02g; aniline blue 0.1-0.4g; calcium citrate tetrahydrate 0.01-0.2g; and water added to 1000ml. The preparation method comprises the following steps of: weighting the malt extract, beef heart for infusion, magnesium sulfate, agar, gentamycin sulfate, aniline blue, calcium citrate tetrahydrate, allithiamine, and glycocholic acid and dissolving the components into an orchis decoction; uniformly blending, dripping the water until constant volume, and sterilizing the mixture and then sub-packaging the mixture. Compared with the prior art, the detection result of the candida from the stool specimens is reliable, and the isolation rate of the candida is high.
Description
Technical field
The present invention relates to check the microorganism culturing field, be specifically related to a kind of candidiasis for stool sample and select substratum.
Background technology
Microorganism culturing is a kind of technology that makes the microorganism growth breeding with manual method, be the organism (as cell or bacterium etc.) that promotes or suppress certain type and the substratum of design utilizes this substratum needed microorganism can be separated from the microorganism that mixes and select substratum.Candidiasis is modal conditioned pathogen in the fungi, claims candiyeast again, and it often parasitizes people's skin, oral cavity, vagina and intestinal mucosa etc. and locates, and the micro-ecological environment imbalance when human body immune function lowly or is normally lived away from home the position causes moniliosis easily.Present oidiomycetic Clinical Laboratory means mainly are Gram stainings, but have 20% candidiasis Gram staining the positive to detect.And as needing definite oidiomycetic kind of differentiating, just need to cultivate, and differentiate with regard to its colony morphology characteristic.But at present clinical used Mycophyta selects the candidiasis of clinical samples such as substratum is mainly used in urinating, phlegm, blood, bile, ascites to separate, as potato dextrose agar (PDA) substratum, low for the candidiasis separation rate in the stool sample, its reason is that intestinal bacteria in the ight soil etc. are faster than the candidiasis growth on present fungi culture medium more, the very fast substratum that covered, candidiasis can't grow.
Summary of the invention
Technical assignment of the present invention is at above the deficiencies in the prior art, provides a kind of stool sample that can be used in, the selection substratum high to the candidiasis separation rate.
The technical scheme that the present invention solves its technical problem is: a kind of candidiasis for stool sample is selected substratum, and its feature exists, and contains in the prescription of preparation 1000ml substratum:
Fructus Hordei Germinatus soaks powder 3～10g;
OX-heart powder 10～20g;
Sal epsom 1.2～2g;
Agar 15～20g;
Gentamicin sulphate 0.005～0.02g;
Aniline orchid 0.1～0.4g;
Four water citric acid calcium, 0.01～0.2g;
Distilled water adds to 1000ml.
In the prioritization scheme, every 1000ml substratum also contains allithiamine 0.05～0.5g.
In the prioritization scheme, every 1000ml substratum also contains glycocholic acid 0.01～0.1g.
In the prioritization scheme, every 1000ml substratum also contains Herba Orchidis Latifoliae water extract 200～300ml of concentration 200g/L.
A kind of candidiasis for stool sample is selected the preparation method of substratum, it is characterized in that may further comprise the steps:
(1) get Herba Orchidis Latifoliae and added water boil 0.5～1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, gets Herba Orchidis Latifoliae water extract;
(2) getting Fructus Hordei Germinatus soaks powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and is dissolved in the step 1 gained Herba Orchidis Latifoliae water extract, mixing, the distilled water constant volume, 121 ℃, 15min sterilization back packing.
Wherein said Fructus Hordei Germinatus soaks powder, the OX-heart powder provides carbon nitrogen source and energy; Sal epsom is buffer reagent, and can destroy antimycotic rhzomorph residual in the sample; Agar is the peptizer of substratum; Gentamicin sulphate suppresses varied bacteria growing, has improved oidiomycetic separation rate; The aniline orchid is indicator; Four water citric acid calcium are buffer reagent and activator; Allithiamine is nutrition-fortifying agent, can promote glucose uptake; Glycocholic acid suppresses the Gram-positive bacteria growing; Herba Orchidis Latifoliae is the herb of orchid Mongolia Herba Orchidis Latifoliae, and herb contains carbohydrate 90%, protein 2.5%, and fat 0.4%, 80% in the carbohydrate is phlegmatic temperament and starch, 20% free sugar and robust fibre; In phlegmatic temperament, get semi-lactosi, fructose, seminose, wood sugar and galacturonic acid; Raffinose is arranged, maltose, sucrose, glucose and wood sugar in the free sugar; Be rich in Methionin, Xie Ansuan and palmitinic acid in the protein at 15 kinds of interior lipid acid, its water extract is good nutrition-fortifying agent, and the effect that suppresses the intestinal bacilli growth is arranged.
The present invention has the characteristics reliable, that the candidiasis separation rate is high that detect compared with prior art.
Embodiment
Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.
Embodiment 1, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 3g; OX-heart powder 20g; Sal epsom 1.2g; Agar 15g; Gentamicin sulphate 0.02g; The blue 0.1g of aniline; Four water citric acid calcium 0.2g; Distilled water adds to 1000ml.The preparation method: get Fructus Hordei Germinatus and soak powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate and be dissolved in the distilled water, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 2, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 10g; OX-heart powder 10g; Sal epsom 2g; Agar 20g; Gentamicin sulphate 0.005g; The blue 0.4g of aniline; Four water citric acid calcium 0.01g; Distilled water adds to 1000ml.The preparation method: get Fructus Hordei Germinatus and soak powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate and be dissolved in the distilled water, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 3, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 3g; OX-heart powder 20g; Sal epsom 1.2g; Agar 15g; Gentamicin sulphate 0.02g; The blue 0.1g of aniline; Four water citric acid calcium 0.2g, allithiamine 0.05g, glycocholic acid 0.1g; Distilled water adds to 1000ml.The preparation method: get Fructus Hordei Germinatus and soak powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and be dissolved in the distilled water, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 4, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 10g; OX-heart powder 10g; Sal epsom 2g; Agar 20g; Gentamicin sulphate 0.005g; The blue 0.4g of aniline; Four water citric acid calcium 0.01g, allithiamine 0.5g, glycocholic acid 0.01g; Distilled water adds to 1000ml.The preparation method: get Fructus Hordei Germinatus and soak powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and be dissolved in the distilled water, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 5, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 6g; OX-heart powder 15g; Sal epsom 1.5g; Agar 18g; Gentamicin sulphate 0.01g; The blue 0.3g of aniline; Four water citric acid calcium 0.1g, allithiamine 0.3g, glycocholic acid 0.05g; Distilled water adds to 1000ml.The preparation method: get Fructus Hordei Germinatus and soak powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and be dissolved in the distilled water, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 6, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 3g; OX-heart powder 20g; Sal epsom 1.2g; Agar 15g; Gentamicin sulphate 0.02g; The blue 0.1g of aniline; Four water citric acid calcium 0.2g, allithiamine 0.05g, glycocholic acid 0.1g, the Herba Orchidis Latifoliae water extract 200ml of concentration 200g/L; Distilled water adds to 1000ml.The preparation method: (1) is got the 40g Herba Orchidis Latifoliae and was added water boil 0.5 hour, filters, and gets filtrate, and according to filtrate volume, taking to concentrate or adding water adjustment volume is 200g/L to concentration, gets Herba Orchidis Latifoliae water extract 200ml; (2) getting Fructus Hordei Germinatus soaks powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and is dissolved in the step 1 gained Herba Orchidis Latifoliae water extract, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 7, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 10g; OX-heart powder 10g; Sal epsom 2g; Agar 20g; Gentamicin sulphate 0.005g; The blue 0.4g of aniline; Four water citric acid calcium 0.01g, allithiamine 0.5g, glycocholic acid 0.01g, the Herba Orchidis Latifoliae water extract 300ml of concentration 200g/L; Distilled water adds to 1000ml.The preparation method: (1) is got the 60g Herba Orchidis Latifoliae and was added water boil 1 hour, filters, and gets filtrate, and adjusting volume is 200g/L to concentration, gets Herba Orchidis Latifoliae water extract 300ml; (2) getting Fructus Hordei Germinatus soaks powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and is dissolved in the step 1 gained Herba Orchidis Latifoliae water extract, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Embodiment 8, and contain in the prescription of preparation 1000ml substratum: Fructus Hordei Germinatus soaks powder 6g; OX-heart powder 15g; Sal epsom 1.5g; Agar 18g; Gentamicin sulphate 0.01g; The blue 0.3g of aniline; Four water citric acid calcium 0.1g, allithiamine 0.3g, glycocholic acid 0.05g, the Herba Orchidis Latifoliae water extract 250ml of concentration 200g/L; Distilled water adds to 1000ml.The preparation method: (1) is got the 50g Herba Orchidis Latifoliae and was added water boil 0.8 hour, filters, and gets filtrate, and adjusting volume is 200g/L to concentration, gets Herba Orchidis Latifoliae water extract 300ml; (2) getting Fructus Hordei Germinatus soaks powder, OX-heart powder, sal epsom, agar, aniline orchid, four water citric acid calcium, gentamicin sulphate, allithiamine, glycocholic acid and is dissolved in the step 1 gained Herba Orchidis Latifoliae water extract, mixing, distilled water is settled to 1000ml, and 121 ℃, 15min sterilization back packing.
Gained candidiasis of the present invention is selected substratum to have for stool sample and detects the characteristics reliable, that the candidiasis separation rate is high, and for clinical data fully proves, pertinent data is as follows.
1 object and method.
1.1 medium preparation: establish 4 groups altogether, be respectively embodiment 8 scheme groups, embodiment 5 scheme groups, embodiment 2 scheme group and control groups, control group is potato dextrose agar (PDA) substratum.
1.2 cultural method: gather outpatient service or the diarrhea patient fresh excreta of being in hospital, be suspended in 5ml0.9% physiological saline, in separating plate, cultivate 24h with the transfering loop sectional streak for 37 ℃, the tiny bacterium colony of picking carries out the Pseudomonas evaluation.
1.3 statistical analysis carries out statistical study with SPSS13.0, P＜0.05 expression has the significance meaning.
2 results: at 969 parts of stool samples, separation also is accredited as oidiomycetic, embodiment 8 scheme groups are 167 strains, embodiment 5 scheme groups are 158 strains, embodiment 2 scheme groups are 155 strains, control group PDA substratum is 124 strains, learns by statistics and handles, and various embodiments of the present invention group and control group relatively have notable difference (embodiment 8 scheme group P=0.006; Embodiment 5 scheme group P=0.029; Embodiment 2 scheme group P=0.045).The result shows, the obviously high existing PDA substratum that is usually used in fungus culture of embodiment of the invention candidiasis separation rate.
Claims (2)
1. a candidiasis that is used for stool sample is selected substratum, and its feature exists, and contains in the prescription of preparation 1000ml substratum:
Fructus Hordei Germinatus soaks powder 6g;
OX-heart powder 15g;
Sal epsom 1.5g;
Agar 18g;
Gentamicin sulphate 0.01g;
The blue 0.3g of aniline;
Four water citric acid calcium 0.1g;
Allithiamine 0.3g;
Glycocholic acid 0.05g;
Distilled water adds to 1000ml.
2. a kind of candidiasis for stool sample according to claim 1 is selected substratum, it is characterized in that every 1000ml substratum also contains the Herba Orchidis Latifoliae water extract 250ml of concentration 200g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210056483 CN102605037B (en) | 2012-03-06 | 2012-03-06 | Candida selection medium for stool specimens and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210056483 CN102605037B (en) | 2012-03-06 | 2012-03-06 | Candida selection medium for stool specimens and preparation method thereof |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310289470.XA Division CN103397072B (en) | 2012-03-06 | 2012-03-06 | Candida selective culture medium for feces specimen and preparation method thereof |
| CN201310289232.9A Division CN103397071B (en) | 2012-03-06 | 2012-03-06 | Candida selective culture medium for feces specimen and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102605037A CN102605037A (en) | 2012-07-25 |
| CN102605037B true CN102605037B (en) | 2013-08-28 |
Family
ID=46522805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210056483 Expired - Fee Related CN102605037B (en) | 2012-03-06 | 2012-03-06 | Candida selection medium for stool specimens and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102605037B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277988A (en) * | 2014-09-29 | 2015-01-14 | 青岛康和食品有限公司 | Candida chromogenic culture medium and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948902A (en) * | 2010-09-10 | 2011-01-19 | 中国检验检疫科学研究院 | Candida chromogenic medium, detection kit and detection method |
-
2012
- 2012-03-06 CN CN 201210056483 patent/CN102605037B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948902A (en) * | 2010-09-10 | 2011-01-19 | 中国检验检疫科学研究院 | Candida chromogenic medium, detection kit and detection method |
Non-Patent Citations (2)
| Title |
|---|
| 李斯特氏菌的选择培养基和分离方法;杨其智等;《中国人兽共患病杂志》;19921231(第1期);第52－53页 * |
| 杨其智等.李斯特氏菌的选择培养基和分离方法.《中国人兽共患病杂志》.1992,(第1期),第52、53页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102605037A (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105943577B (en) | The microbial fermentation of autonomic drug | |
| CN103330258B (en) | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof | |
| CN101870740B (en) | Morchellaconica extracellular polysaccharide extractive and preparation method and application thereof | |
| CN104357355A (en) | Bacillus natto capable of producing MK-7 and application of bacillus natto | |
| CN108244432A (en) | A kind of fermentation Cordyceps militaris probiotic beverage and preparation method thereof | |
| CN108402175A (en) | A kind of dendrobium candidum health care yoghourt and preparation method thereof | |
| TWI460272B (en) | Method for producing gamma-aminobutyric acid by fermentation with grain-based solid substrates used in producing cordyceps species and the application thereof | |
| CN108486002B (en) | Momordica grosvenori endophyte strain capable of producing exopolysaccharides, method for producing exopolysaccharides and application of exopolysaccharides | |
| CN105062899B (en) | One plant of two spore intends the extraction and application of Aode mushroom bacterial strain and its Thick many candies | |
| CN106434489A (en) | High-wine-yield Klebsiella pneumoniae and application thereof | |
| CN102605037B (en) | Candida selection medium for stool specimens and preparation method thereof | |
| CN104046584A (en) | Bifidobacterium adolescentis bacteriocin as well as production method and special production strain of bifidobacterium adolescentis bacteriocin | |
| CN104046585A (en) | Bifidobacterium animal bacteriocin, production method thereof and specific production strain | |
| CN103397072B (en) | Candida selective culture medium for feces specimen and preparation method thereof | |
| CN115838661A (en) | Lactobacillus plantarum magpie gentlemen 18, lactobacillus plantarum preparation and application thereof | |
| CN105535035A (en) | Inonotus obliquus fermentation culture composition and preparation method thereof | |
| CN105725215B (en) | A kind of lactic acid bacteria oral liquor and preparation method thereof | |
| CN109136151A (en) | A kind of composite plant Lemonal and its application with reduction blood glucose function | |
| CN103397071B (en) | Candida selective culture medium for feces specimen and preparation method thereof | |
| CN104293710B (en) | Streptomycete strains and combined application thereof in prevention and treatment of pepper seedling blight | |
| CN101886043B (en) | Composite yoghurt starter and application thereof | |
| CN104911131A (en) | Enterococcus mundtii and application thereof in low-temperature ensiling | |
| CN106883997A (en) | Pediococcus pentosaceus JQI-7 bacterial strains and its application | |
| CN109161501A (en) | A kind of feeding bacillus licheniformis and its application | |
| Abed Almjalawi et al. | Determination of optimum conditions for the production of the mother culture of the medicinal wild mushroom, Agaricus bellanniae isolated from hot Iraqi environment (Baghdad Governorate) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130828 Termination date: 20140306 |