CN102604942A - Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker - Google Patents
Molecular marker of wheat powdery mildew-resistant gene Pm45 and application of molecular marker Download PDFInfo
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Abstract
The invention belongs to the field of molecular genetics and relates to a molecular marker of a wheat powdery mildew-resistant gene Pm45 and application of molecular marker. The molecular marker is selected from any one of MAG6139, MAG6140 and BARC123, and the genetic distance from the MAG6139, MAG6140 and BARC123 to the Pm45 is 0.7cm, 2.7cm and 5.2cm respectively. A Pm45-containing single plant is selected according to the molecular marker MAG6139, the molecular marker MAG6140 and the molecular marker BARC123, and then Pm45-containing disease-resistant varieties with different genetic backgrounds can be selected and cultivated. The Pm45-containing plants can be simply, conveniently and quickly selected by using the molecular marker tightly linked with the Pm45. Due to the application of the molecular marker MAG6139, the molecular marker MAG6140 and the molecular marker BARC123, the time and the labor for selecting a disease-resistant material can be greatly saved, and the accuracy of pre-detection can be improved.
Description
Technical field
The invention belongs to the molecular genetics field, relate to molecule marker and the application thereof of powdery mildew resistance gene in wheat Pm45.
Background technology
Wheat is global topmost food crop, also is second largest food crop of time paddy rice only in China.At present, the annual output of world wheat is about 6.2 hundred million tons, and 1/5 energy of necessary for human is provided, and the production of wheat is concerning human survival.Common wheat is allohexaploid species, contains A, B, three different dyeing bodies of D group, has with the wheat cdna group and has contained abundant genetic resources in homology or homeologous wild species and the original species.Utilizing allelic variation excellent in the wheat germplasm resource is the key factor that realizes wheat stable yields, raising the output.
Wheat powdery mildew is that the cereal powdery mildew wheat specialized form Blumeria graminis f.sp.tritici by Ascomycetes camber obligatory parasitism infects and a kind of gas of causing passes sick.The wheat powdery mildew that it causes is by a kind of serious wheat leaf diseases.After the infection process wheat, absorb plant nutrition, the photosynthetic capacity of wheat plant is descended even completely lose, finally cause mature spike number, grain number per spike and thousand seed weight to descend, can cause wheat yield loss 5%～34%, can reach 45% even total crop failure when serious.
In numerous wheat powdery mildew prophylactico-therapeutic measuress, cultivate and promote disease-resistant variety economical and effective and environmental protection the most.The mildew-resistance utilization mainly is the quality resistant gene in the current production.But; Because Powdery Mildew quality resistance has the microspecies specialization; Nontoxic gene of pathogenic bacteria and resistant gene carry out coevolution, and big area is promoted the use of single disease-resistant variety and is easy to cause producing new toxicity microspecies on producing, thereby makes entrained its resistance of disease-resistant gene forfeiture.From the wheat germplasm resource, excavate with to utilize new anti-white powder gene be the wheat genetic scholar with breeding man long-term objective and challenge.
Summary of the invention
The objective of the invention is above-mentioned deficiency, the molecule marker of powdery mildew resistance gene in wheat Pm45 is provided to prior art.
Another object of the present invention provides the molecule marker of this powdery mildew resistance gene in wheat Pm45 and uses.
The object of the invention can be realized through following technical scheme:
The molecule marker of powdery mildew resistance gene in wheat Pm45, described molecule marker is selected from any one among MAG6139, MAG6140 or the BARC123;
Described molecule marker MAG6139:
The left end primer sequence is SEQ ID NO.1,
The right-hand member primer sequence is SEQ ID NO.2,
Amplified production is 880bp, and this molecule marker is the codominance molecule marker from common wheat germplasm D576DS karyomit(e), and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 0.7cM;
Described molecule marker MAG6140:
The left end primer sequence is SEQ ID NO.3,
The right-hand member primer sequence is SEQ ID NO.4,
Amplified fragments 495bp, this molecule marker is from common wheat germplasm D576DS karyomit(e), and MAG6140 is the dominance molecule marker, and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 2.7cM;
Described molecule marker BARC123:
The left end primer sequence is SEQ ID NO.5,
The right-hand member primer sequence is SEQ ID NO.6,
Amplified fragments 420bp, this molecule marker is from common wheat germplasm D576DS karyomit(e), and BARC123 is the dominance molecule marker, and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 5.2cM.
The application in identification of described molecule marker mildew-resistance gene in the wheat germplasm resource.
The molecule marking method of powdery mildew resistance gene in wheat Pm45, with the following any a pair of molecule marker primer PCR wheat cdna group DNA to be checked that increases, and detect amplified production:
1) molecule marker MAG6139:
The left end primer sequence is SEQ ID NO.1,
The right-hand member primer sequence is SEQ ID NO.2;
2) molecule marker MAG6140:
The left end primer sequence is SEQ ID NO.3,
The right-hand member primer sequence is SEQ ID NO.4;
3) molecule marker BARC123:
The left end primer sequence is SEQ ID NO.5,
The right-hand member primer sequence is SEQ ID NO.6;
If can amplify the amplified fragments of 880bp with primer MAG6139; Perhaps can amplify the amplified fragments of 495bp with primer MAG6140; Perhaps can amplify the amplified fragments of 420bp, indicate that then there is mildew-resistance gene Pm45 in wheat to be checked with primer BARC123.
A kind of method of screening powdery-mildew-resistance wheat; With above-mentioned primer to one of the amplification wheat cdna group DNA to be checked; If can amplify the amplified fragments of 880bp with primer MAG6139; Perhaps can amplify the amplified fragments of 495bp, perhaps can amplify the amplified fragments of 420bp, indicate that then this wheat to be checked is the powdery-mildew-resistance wheat that has mildew-resistance Pm45 with primer BARC123 with primer MAG6140.
The molecule marker of above-mentioned powdery mildew resistance gene in wheat Pm45 can obtain through following method:
(1) D57 with raise wheat the 158F2 establishment and the phenotypic evaluation in generation:
(1) common wheat germplasm D57
With raise wheat 158 (♀) and hybridize and obtain hybrid F
1, F
1Selfing produces F
2Colony.
(2) F
2Coil in the cave for colony's single-strain planting, place the greenhouse, daytimes 22, degree was 14 hours, and evenings 18, degree was 10 hours.One leaf, one heart stage inoculation Powdery Mildew is inoculated and is carried out the resistance investigation after 7 days.Qualification result is seen table 1.
(3) F
2For the land for growing field crops of transplanting seedlings after the individual plant investigation, selfing gained seed is F
3Family.Each F
3Select 18 to carry out the filial generation test in the family.Table 1 between qualification result.
(4) pass through F
2And F
2: 3Carry out genetic analysis, infer the number of the disease-resistant gene that carries in the disease-resistant germplasm.
(2) polymorphic molecular marker screening
(5) with the disease-resistant F of 6 strains
2The blade balanced mix Cheng Kangchi of individual plant, the susceptible F of 6 strains
2The blade balanced mix of individual plant becomes the sense pond.Extract the DNA in disease-resistant parent D57, susceptible parent Yang Mai 158, anti-pond and sense pond with SDS method (Ma and Sorrells, 1995), use simple repeated sequence mark SSR and BSA (Bulk segregant analysis) bonded method and carry out the polymorphum screening.
(6) at first utilize 412 pairs of SSR primers to D57, raise wheat 158, anti-pond and the polymorphum screening is carried out in the sense pond.
The PCR reaction volume is 25 microlitres, 10 * buffer, 2.5 microlitres wherein, and 25mM MgCl21.5 microlitre, 2.5mM dNTPs 2 microlitres, Taq enzyme (5 units/microlitre) 0.2 microlitre, each 0.5 microlitre of 10 μ M primers (F/R), template DNA 20 nanograms add water to 25 microlitres;
After the SSR reaction system is 94 ℃ of preparatory sex change 3min of DNA, 94 ℃ of sex change 1min, 50-65 ℃ (look primer and decide) annealing 1min, 72 ℃ are extended exhibition 1min, circulates 35 times, last 72 ℃ of extension 10min;
In the enterprising performing PCR amplification of pcr amplification appearance, amplified production carries out electrophoretic separation on 8% non-denaturing polyacrylamide gel, on ultraviolet transilluminator, takes a picture the record result then:
Be chosen between the parent and F
2For amplifying the identical primer that multiformity is arranged between the R of colony, S pond, with 4 pairs of the chain candidate molecular markers of Pm45;
(7) select to separate than the F that is 3: 1
2: 3Family D57-1 and D57-2 expand as segregating population and carry out that resistance is identified and the filial generation test, with above-mentioned 4 pairs of molecule markers at F
3: 4The genotype data of each individual plant of colony is obtained in amplification in the colony;
(3) molecule marker obtains
(8) according to chain exchange rule, the F that utilizes amplification to obtain
3: 4Each individual plant genotype data of colony and resistance identify that the resistance data that obtains makes up the linkage map of 4 pairs of molecule markers and D57-6D gene; Used software is Mapmaker Macintosh V 2.0; Obtained and the closely linked molecule marker BARC123 of Pm45, CFD80, CFD190 and GDM108, the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record they and Pm45 gene is respectively 5.2cM, 0.7cM, 2.4cM and 5.5cM;
(9) exploitation of molecule marker; Because above-mentioned four marks all are located on the chromosomal 6DS2bin of wheat 6D; EST BG262421 and BE44520 that utilization is positioned at wherein are converted into STS mark MAG6139 and MAG6140, after their PCR product is cut through RsaI and TaqI enzyme respectively, in anti-sense parent and anti-sense pond, the polymorphic of unanimity are arranged; Be positioned to the Pm45 both sides, respectively apart from Pm450.7cM and 2.7cM;
(4) acquisition of anti-spectrum, to containing the different powdery mildew physiological strain of pure lines inoculation of Pm45, the result shows 15 Powdery Mildew physiological strain performances disease-resistant, has good disease-resistant performance.
Beneficial effect:
The present invention has identified a mildew-resistance gene Pm45 who is positioned on the wheat 6DS in the world first, has widened the variety of powdery mildew resistance gene in wheat.The disease-resistant performance that Pm45 is excellent makes it in the mildew-resistance breeding, significant values arranged.And obtained molecule marker MAG6139 closely linked, MAG6140 and BARC123 with it, can quicken the application of disease-resistant gene Pm45 in wheat breeding for disease resistance.
1, identified the mildew-resistance gene Pm45 that is positioned on the wheat 6D karyomit(e) first.Find also not to be positioned at the mildew-resistance gene on the wheat 6D in more than 70 powdery mildew resistance gene in wheat in 40 sites at present, the variety of wheat powdery mildew gene has been widened in the discovery of Pm45.
2, the anti-spectrum of Pm45 is measured, the result shows whole 15 the Powdery Mildew physiological strains in anti-this laboratory of Pm45, has excellent resistance, is disease-resistant gene good in the Powdery Mildew breeding.
3, obtained and the closely linked molecule marker MAG6139 of powdery mildew resistance gene in wheat Pm45, MAG6140 and BARC123.Chain with Pm45 in all known molecule markers is MAG6139 the most closely, and genetic distance is merely 0.7cM, also is unique codominance molecule marker simultaneously, can help this gene in commercial variety, to shift and with other disease-resistant gene polymerization.
4, identify conveniently.That molecule marker has is easy to detect, stable amplification, advantage such as easy.Detect powdery mildew resistance gene in wheat Pm45 with mark MAG6139, the existence that can confirm Pm45 whether and existence, and prediction powder mildew resistance, and then accelerate to shift the utilization of Pm45.
5, the present invention proposes the method for utilizing mildew-resistance gene in the wheat germplasm resource.
Description of drawings
Fig. 1 molecule marker amplification banding pattern
The a:BARC123 banding pattern that increases wherein 1,2,3,4 is respectively disease-resistant variety D57, wheat 158, anti-pond and sense pond are raised in commercial variety, and M:pUC19/MspI, arrow indication are 420bp specific amplified band;
The b:MAG6139 banding pattern that increases wherein 1,2,3,4 is respectively disease-resistant variety D57, wheat 158, anti-pond and sense pond are raised in commercial variety, M:pUC19/MspI, and the arrow indication is a 880bp specific amplified band in the swimming lane 1;
The c:MAG6140 banding pattern that increases wherein 1,2,3,4 is respectively disease-resistant variety D57, wheat 158, anti-pond and sense pond are raised in commercial variety, and M:pUC19/MspI, arrow indication are 495bp specific amplified band;
The genetic linkage map of Fig. 2 MAG6139, MAG6140 and BARC123 and powdery mildew resistance gene in wheat Pm45, the left side are the map distance between mark.
Embodiment
(1) D57 with raise wheat 158F
2The establishment in generation and phenotypic evaluation:
(1) common wheat germplasm D57
With raise wheat 158 (♀) and hybridize and obtain hybrid F
1, F
1Selfing produces F
2Colony.
(2) F
2Coil in the cave for colony's single-strain planting, place the greenhouse, daytimes 22, degree was 14 hours, and evenings 18, degree was 10 hours.One leaf, one heart stage inoculation Powdery Mildew is inoculated and is carried out the resistance investigation after 7 days.Qualification result is seen table 1.
(3) F
2For the land for growing field crops of transplanting seedlings after the individual plant investigation, selfing gained seed is F
3Family.Each F
3Select 18 to carry out the filial generation test in the family.Table 1 between qualification result.
Raise wheat 158 * D57F after table 1Bgt18 and the Bgt19 inoculation
2And F
2: 3The resistance of family is separated
R, S, RR, Rr, rr represent disease-resistant individual plant, susceptible individual plant respectively, the disease-resistant family of isozygotying, separate family, the susceptible family of isozygotying.χ
2 0.05，1＝3.84，χ
2 0.05，2＝5.99
(4) pass through F
2And F
2: 3Carry out genetic analysis, find to carry in the disease-resistant germplasm disease-resistant gene of two independent inheritances.
(2) polymorphic molecular marker screening
(1) with the disease-resistant F of 6 strains
2The blade balanced mix Cheng Kangchi of individual plant, the susceptible F of 6 strains
2The blade balanced mix of individual plant becomes the sense pond.Extract the DNA in disease-resistant parent D57, susceptible parent Yang Mai 158, anti-pond and sense pond with SDS method (Ma and Sorrells, 1995), use simple repeated sequence mark SSR and BSA (Bulk segregant analysis) bonded method and carry out the polymorphum screening.
(2) at first utilize 412 pairs of SSR primers (barc:Song QJ, Shi JR, Singh S; Fickus EW; Costa JM, Lewis J, Gill BS; Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat.Theor Appl Genet 110:550-560; Gwm:
MS; Korzun V; Wendehake K, Plaschke J, TixerMH; Leroy P, Ganal MW (1998) A microsatellite map of wheat.Genetics 149:2007-2023; Wmc:Wheat Microsatellite Consortium (http://wheat.pw.usda.gov); Cfa, gpw:Sourdille ' s lab (http://wheat.pw.usda.gov); Cfd:Guyomarc ' h H; Sourdille P; Charmet G; Edwards KJ, Bernard M (2002) Characterization of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat.TheorAppl Genet 104:1164-1172; Gdm:Pestsova E; Ganal MW,
MS (2000) Isolation and mapping of microsatellite markers speciWc for the D genome of bread wheat.Genome 43:689-697) to D57, raise wheat 158, anti-pond and sense pond and carry out the polymorphum screening.
The PCR reaction volume is 25 microlitres, 10 * buffer, 2.5 microlitres wherein, and 25mM MgCl21.5 microlitre, 2.5mM dNTPs 2 microlitres, Taq enzyme (5 units/microlitre) 0.2 microlitre, each 0.5 microlitre of 10 μ M primers (F/R), template DNA 20 nanograms add water to 25 microlitres;
After the SSR reaction system is 94 ℃ of preparatory sex change 3min of DNA, 94 ℃ of sex change 1min, 50-65 ℃ (look primer and decide) annealing 1min, 72 ℃ are extended exhibition 1min, circulates 35 times, last 72 ℃ of extension 10min;
Wherein SSR mark BARC123 anti-sense parent with anti-feel detected between the pond that consistent polymorphic (Fig. 1 a).Positioning result (Somers DJ according to Somer and Song; Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.) .Theor Appl Genet 109:1105-1114; Song QJ, Shi JR, Singh S; Fickus EW, Costa JM, Lewis J; Gill BS; Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat.Theor Appl Genet 110:550-560), BARC123 has the amplification site on 6D and 7B.In order further to confirm its position, we resist the sense pond to analyze with near other mark the corresponding site on 6D and the 7B karyomit(e) again, find CFD80, CFD190, GDM108 the anti-parent of sense with resist feel can detect between the pond consistent polymorphic.Because BARC123, CFD80, CFD190 and GDM108 are positioned on the 6DS, the disease-resistant gene that we infer among the D57 is positioned 6DS, and with its called after PmD57-6D.
(3) result and analysis:
(1) in order to make up the molecule marker linkage map of PmD57-6D, we have chosen 2 anti-senses by 3: 1 isolating F
3Family does further analysis.And to having carried out identifying that again the result still meets separation in 3: 1 behind each strain system expansion population size.To by these F
3Individual plant deutero-F in the family
3: 4Family is carried out resistance and is identified that their resistance is separated and met 1: 2: 1 ratio (table 2).
Table 2 contains the resistance of monogenic separation family and separates
(2) according to chain exchange rule; With D57-1 and two familys of D57-2 as mapping population; Analyze the relation of BARC123, CFD80, CFD190, these four SSR marks of GDM108 and disease resistance; Find that they are all chain with Pm45, utilize software Mapmaker Macintosh V2.0 (general mapping software), made up the molecule marker linkage map (Fig. 2) of Pm45.Found and the closely linked molecule marker BARC123 of Pm45, CFD80, CFD190 and GDM108, the genetic distance of they and Pm45 is respectively 5.2cM, 0.7cM, 2.4cM and 5.5cM.Four SSR marks all show dominance, wherein have only the entrained banding pattern of BARC123 from disease-resistant variety D57, can be used for the secondary transfer of Pm45.
(3) exploitation of molecule marker; Owing to all be located on the chromosome of wheat bin6DS2 with closely linked several marks of Pm45; According to STS mark MAG6139 and the MAG6140 that the EST BG262421 and the BE44520 that are positioned on the 6DS2 transform, after their PCR product is cut with the TaqI enzyme through RsaI respectively, in anti-sense parent and anti-sense pond, unanimity polymorphic (Fig. 1 b, c) is arranged; Be positioned to the Pm45 both sides, respectively apart from Pm450.7cM and 2.7cM.Wherein MAG6140 is the dominant marker, and banding pattern can be used for the transfer of Pm45 among the disease-resistant material D57, and MAG6139 is the codominant marker, can be used for the seed selection of disease-resistant gene more easily.
(4) acquisition of anti-spectrum, to containing the different powdery mildew physiological strain of pure lines inoculation of Pm45, the result shows 15 Powdery Mildew physiological strain performances disease-resistant, has good disease-resistant performance.
Contain abundant genetic resources in the wheat germplasm resource, utilize these genetic resourceses to have vital role for wheat.The present invention is through hybridizing disease-resistant germplasm D57 and susceptible variety; Confirm to contain in the disease-resistant material disease-resistant gene of two independent inheritances through genetic analysis; Through molecular marker screening, and make up segregating population, find that one of them disease-resistant gene is positioned on the wheat 6DS karyomit(e); Be a unique up to now mildew-resistance gene that is positioned on the 6D, utilize molecular marker method to make up the genetic linkage map of disease-resistant gene Pm45 simultaneously through making up segregating population.Anti-spectrum test finds that Pm45 has excellent powder mildew resistance, is the good anti-source of wheat anti-powdery mildew breeding.Select to contain the Pm45 individual plant through molecule marker MAG6139, MAG6140 and BARC123, can select and contain Pm45 but the different disease-resistant variety of genetic background.In the method for traditional breeding method, owing to do not know the heredity and the chain molecule marker thereof of disease-resistant material, per generation shifts all needs resistance to identify; Waste time and energy, therefore, the known and closely linked molecule marker of Pm45; The plant that can simple and efficient screening contains Pm45; The application of molecule marker MAG6139, MAG6140 and BARC123, the screening time and the labour that can save disease-resistant material greatly, and strengthen prediction accuracy.
Claims (4)
1. the molecule marker of powdery mildew resistance gene in wheat Pm45 is characterized in that described molecule marker is selected from any one among MAG6139, MAG6140 or the BARC123;
Described molecule marker MAG6139:
The left end primer sequence is SEQ ID NO.1,
The right-hand member primer sequence is SEQ ID NO.2,
Amplified production is 880bp, and this molecule marker is the codominance molecule marker from common wheat germplasm D576DS karyomit(e), and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 0.7cM;
Described molecule marker MAG6140:
The left end primer sequence is SEQ ID NO.3,
The right-hand member primer sequence is SEQ ID NO.4,
Amplified fragments 495bp, this molecule marker is from common wheat germplasm D576DS karyomit(e), and MAG6140 is the dominance molecule marker, and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 2.7cM;
Described molecule marker BARC123:
The left end primer sequence is SEQ ID NO.5,
The right-hand member primer sequence is SEQ ID NO.6,
Amplified fragments 420bp, this molecule marker is from common wheat germplasm D576DS karyomit(e), and BARC123 is the dominance molecule marker, and the genetic distance that utilizes Mapmaker Macintosh V 2.0 to record with the Pm45 gene is 5.2cM.
2. the application in identification of the described molecule marker of claim 1 mildew-resistance gene in the wheat germplasm resource.
3. the molecule marking method of powdery mildew resistance gene in wheat Pm45 is characterized in that: with the following any a pair of molecule marker primer PCR wheat cdna group DNA to be checked that increases, and detect amplified production:
1) molecule marker MAG6139:
The left end primer sequence is SEQ ID NO.1,
The right-hand member primer sequence is SEQ ID NO.2;
2) molecule marker MAG6140:
The left end primer sequence is SEQ ID NO.3,
The right-hand member primer sequence is SEQ ID NO.4;
3) molecule marker BARC123:
The left end primer sequence is SEQ ID NO.5,
The right-hand member primer sequence is SEQ ID NO.6; If can amplify the amplified fragments of 880bp with primer MAG6139; Perhaps can amplify the amplified fragments of 495bp with primer MAG6140; Perhaps can amplify the amplified fragments of 420bp, indicate that then being fond of wheat exists mildew-resistance gene Pm45 with primer BARC123.
4. method of screening powdery-mildew-resistance wheat; It is characterized in that with the described primer of claim 1 to one of the amplification wheat cdna group DNA to be checked; If can amplify the amplified fragments of 880bp with primer MAG6139; Perhaps can amplify the amplified fragments of 495bp, perhaps can amplify the amplified fragments of 420bp, indicate that then this wheat to be checked is the powdery-mildew-resistance wheat that has mildew-resistance Pm45 with primer BARC123 with primer MAG6140.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104313021A (en) * | 2014-10-21 | 2015-01-28 | 山西省农业科学院作物科学研究所 | Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker |
| CN105112416A (en) * | 2015-09-28 | 2015-12-02 | 江苏省农业科学院 | Molecular marker primer in close linkage with Hongyoumai powdery mildew resistance gene and application of molecular marker primer |
| CN107619882A (en) * | 2017-11-21 | 2018-01-23 | 山东农业大学 | A kind of wild emmer mildew-resistance gene molecular labeling and its application |
| CN109152345A (en) * | 2016-02-22 | 2019-01-04 | 贝霍种子有限公司 | Powdery mildew resistance gene in carrot |
| CN109355426A (en) * | 2018-12-17 | 2019-02-19 | 山西省农业科学院作物科学研究所 | Detect diagnostic molecular labeling and its application of powdery mildew resistance gene in wheat Pm2a |
| CN110923352A (en) * | 2019-12-02 | 2020-03-27 | 河南大学 | KASP marker of wheat powdery mildew resistance gene PmDTM and application thereof |
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2012
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| CN104313021A (en) * | 2014-10-21 | 2015-01-28 | 山西省农业科学院作物科学研究所 | Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker |
| CN105112416A (en) * | 2015-09-28 | 2015-12-02 | 江苏省农业科学院 | Molecular marker primer in close linkage with Hongyoumai powdery mildew resistance gene and application of molecular marker primer |
| CN105112416B (en) * | 2015-09-28 | 2018-03-30 | 江苏省农业科学院 | Molecular labeling primer and application with red common house centipede wheat mildew-resistance gene close linkage |
| CN109152345A (en) * | 2016-02-22 | 2019-01-04 | 贝霍种子有限公司 | Powdery mildew resistance gene in carrot |
| CN109152345B (en) * | 2016-02-22 | 2021-11-09 | 贝霍种子有限公司 | Powdery mildew resistance gene in carrot |
| CN107619882A (en) * | 2017-11-21 | 2018-01-23 | 山东农业大学 | A kind of wild emmer mildew-resistance gene molecular labeling and its application |
| CN109355426A (en) * | 2018-12-17 | 2019-02-19 | 山西省农业科学院作物科学研究所 | Detect diagnostic molecular labeling and its application of powdery mildew resistance gene in wheat Pm2a |
| CN109355426B (en) * | 2018-12-17 | 2021-10-15 | 山西省农业科学院作物科学研究所 | Diagnostic molecular marker for detecting powdery mildew resistance gene Pm2a of wheat and application thereof |
| CN110923352A (en) * | 2019-12-02 | 2020-03-27 | 河南大学 | KASP marker of wheat powdery mildew resistance gene PmDTM and application thereof |
| CN110923352B (en) * | 2019-12-02 | 2021-07-23 | 河南大学 | KASP marker of wheat powdery mildew resistance gene PmDTM and application thereof |
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