CN102599062B - Method for rapid propagating tissue of wistaria - Google Patents
Method for rapid propagating tissue of wistaria Download PDFInfo
- Publication number
- CN102599062B CN102599062B CN2012100867690A CN201210086769A CN102599062B CN 102599062 B CN102599062 B CN 102599062B CN 2012100867690 A CN2012100867690 A CN 2012100867690A CN 201210086769 A CN201210086769 A CN 201210086769A CN 102599062 B CN102599062 B CN 102599062B
- Authority
- CN
- China
- Prior art keywords
- culture
- days
- tube plantlet
- test
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention relates to a method for rapid propagating tissue of wistaria, which is realized by the steps of explant selecting, cleaning, primary culturing, sub-culturing and rooting culturing, wherein a special culture medium is selected for the primary culturing, the sub-culturing and the rooting culturing, so that the purposes of high propagation coefficient and good propagation effect are achieved.
Description
Technical field
The present invention relates to the gardening field, be specifically related to a kind of tissue culture and rapid propagation method of Chinese wistaria.
Background technology
(formal name used at school: Wisteria sinensis (Sims) Sweet) belong to the pulse family Wistaria, be that a kind of fallen leaves are climbed up by holding on to the large liane dry hide of prehensile Dark grey to Chinese wistaria, do not split; Flower purple or darkviolet are very beautiful.Chinese wistaria is for warm band and temperate plant, to the strong adaptability of weather and soil, more cold-resistant, can water-fastly wet and hungry soil, and happiness light, more anti-the moon.Chinese wistaria originates in China, and also there are distribution in Korea, Japan.There is distribution North China more, the most common with Hebei, Henan, Shanxi, Shandong.All there is cultivation East China, Central China, south China, northwest and southwest.Generally cultivate in flower garden in South China to the ，Bei Zhi Inner Mongol, Guangdong, for viewing and admiring.Flower can be propagandized vegetable edible, the cauline leaf hyoscine.But the flowers are in blossom, first quarter moon does not wither.Common kind has Wisteria floribunda, silvernine, carbuncle rattan, white jade rattan, Nanjing rattan etc.There are Chinese wistaria town, Chinese wistaria garden in Shanghai, and also there is ancient rattan in Suzhou.
Chinese wistaria is long-lived seeds, the utmost point among the people happiness plantation, and climing the wriggling of adult plants stems prolonged flexing, blooms various, and string a string of flowers order hangs between the greenery tendril, and tall and thin pod is bent before the wind, and Chinese scholar all likes take that it chants the poem picture for subject matter since ancient times.Climb around frame with it in garden, make colored corridor, or climb around withered tree with it, have withered tree to meet the meaning of life.Also can make gainly steep cliff formula potted landscape, be placed in several of height, bookcase top above, full-blown flowers are completely set, and old stake crosswise, does not have charm.
The multiplex cuttage of Chinese wistaria and seed propagation, its reproduction speed is subject to the restriction of maternal material, for meeting the Landscape Application demand, has certain difficulty.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of method for quickly breeding of Chinese wistaria, to make up in gardens the demand endlessly for the Chinese wistaria resource.
For solving the problems of the technologies described above, the object of the present invention is achieved like this:
A kind of Chinese wistaria tissue culture and rapid propagation method, is characterized in that, comprises the following steps:
The foundation of step (1) explant: late April, get Chinese wistaria and give birth to then healthy and strong tender tip stem section, with 1% washing powder solution sterilization, with sterile water, soak 30min;
Step (2) explant is processed: the alcoholic solution with 70% soaks 60s, and aseptic water washing 2 times, drain, then put into 0.1% HgCl
2Soaking disinfection 20min in solution, aseptic water washing 3 times, the gauze suck dry moisture, cut the stem section with leaf bud, standby;
Step (3) is culture just: the stem section that step (2) is cut, be seeded on first culture base, and carry out just culture, first culture based component is MS+GA
30.4mg/l+IAA2mg/l+BA 0.6mg/l+2g/l PVP+ 2% sucrose, medium PH5.9; First culture intensity of illumination 1000Lux, 23 ± 2 ℃ of cultivation temperature, incubation time 35 ± 2 days, proceed to subculture and cultivate;
Step (4) shoot proliferation: the stem section after the first culture that step (3) is obtained moves into and is equipped with in the airtight container of subculture medium, carries out the subculture cultivation; The subculture medium composition is 1/2MS+ GA
30.6mg/l+BA 0.5mg/l+IAA 0.15mg/l+ silver nitrate 2.0mg/l+3% sucrose; 23 ± 2 ℃ of cultivation temperature, light application time 16h/d, Medium's PH Value 5.8～6.0, cultivate 20 days, forms the long budling with 3 stipes of 4cm, is cut into the segment with stipes, and each budling is divided into 2 sections; Every 20 days subcultures like this once, expand numerous test-tube plantlet;
Step (5) culture of rootage: step (4) is expanded to numerous test-tube plantlet and take out, move into and be equipped with in the closed container of root media, carry out culture of rootage, the culture of rootage based component is MS+ GA
30.5mg/l+IBA 0.5mg/l+BA 0.01mg/l+10% sucrose; Test-tube plantlet intensity of illumination in root media is 3000Lux, and 25 ± 2 ℃ of cultivation temperature, be cultured to 30 days, bottle outlet when test-tube plantlet root in medium grows to the new root of 0.5cm;
Step (6) hardening: select healthy and strong test-tube plantlet bottle outlet to be transplanted to vermiculite: in the matrix of the peat composed of rotten mosses=3:1, hardening 40-45 days, air humidity 85-90%, illumination 10000lux.
The invention has the beneficial effects as follows: the tissue-culturing rapid propagation seedling-cultivating method of Chinese wistaria of the present invention has been set up a set of more effective Chinese wistaria fast breeding technique, and subculture cycle is short, and the propagation multiple reaches more than 4 times, and rooting rate reaches 99%.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
The embodiment of the present invention comprises:
A kind of Chinese wistaria tissue culture and rapid propagation method, is characterized in that, comprises the following steps:
The foundation of step (1) explant: late April, get Chinese wistaria and give birth to then healthy and strong tender tip stem section, with 1% washing powder solution sterilization, with sterile water, soak 30min;
Step (2) explant is processed: the alcoholic solution with 70% soaks 60s, and aseptic water washing 2 times, drain, then put into 0.1% HgCl
2Soaking disinfection 20min in solution, aseptic water washing 3 times, the gauze suck dry moisture, cut the stem section with leaf bud, standby;
Step (3) is culture just: the stem section that step (2) is cut, be seeded on first culture base, and carry out just culture, first culture based component is MS+GA
30.4mg/l+IAA2mg/l+BA 0.6mg/l+2g/l PVP+ 2% sucrose, medium PH5.9; First culture intensity of illumination 1000Lux, 23 ± 2 ℃ of cultivation temperature, incubation time 35 ± 2 days, proceed to subculture and cultivate;
Step (4) shoot proliferation: the stem section after the first culture that step (3) is obtained moves into and is equipped with in the airtight container of subculture medium, carries out the subculture cultivation; The subculture medium composition is 1/2MS+ GA
30.6mg/l+BA 0.5mg/l+IAA 0.15mg/l+ silver nitrate 2.0mg/l+3% sucrose; 23 ± 2 ℃ of cultivation temperature, light application time 16h/d, Medium's PH Value 5.8～6.0, cultivate 20 days, forms the long budling with 3 stipes of 4cm, is cut into the segment with stipes, and each budling is divided into 2 sections; Every 20 days subcultures like this once, expand numerous test-tube plantlet;
Step (5) culture of rootage: step (4) is expanded to numerous test-tube plantlet and take out, move into and be equipped with in the closed container of root media, carry out culture of rootage, the culture of rootage based component is MS+ GA
30.5mg/l+IBA 0.5mg/l+BA 0.01mg/l+10% sucrose; Test-tube plantlet intensity of illumination in root media is 3000Lux, and 25 ± 2 ℃ of cultivation temperature, be cultured to 30 days, bottle outlet when test-tube plantlet root in medium grows to the new root of 0.5cm;
Step (6) hardening: select healthy and strong test-tube plantlet bottle outlet to be transplanted to vermiculite: in the matrix of the peat composed of rotten mosses=3:1, hardening 40-45 days, air humidity 85-90%, illumination 10000lux.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in scope of patent protection of the present invention.
Claims (1)
1. a Chinese wistaria tissue culture and rapid propagation method, is characterized in that, comprises the following steps:
The foundation of step (1) explant: late April, get Chinese wistaria and give birth to then healthy and strong tender tip stem section, with 1% washing powder solution sterilization, with sterile water, soak 30min;
Step (2) explant is processed: the alcoholic solution with 70% soaks 60s, and aseptic water washing 2 times, drain, then put into 0.1% HgCl
2Soaking disinfection 20min in solution, aseptic water washing 3 times, the gauze suck dry moisture, cut the stem section with leaf bud, standby;
Step (3) is culture just: the stem section that step (2) is cut, be seeded on first culture base, and carry out just culture, first culture based component is MS+GA
30.4mg/L+IAA2mg/L+6-BA 0.6mg/L+2g/L PVP+ 2% sucrose, Medium's PH Value 5.9; First culture intensity of illumination 1000Lux, 23 ± 2 ℃ of cultivation temperature, incubation time 35 ± 2 days, proceed to subculture and cultivate;
Step (4) shoot proliferation: the stem section after the first culture that step (3) is obtained moves into and is equipped with in the airtight container of subculture medium, carries out the subculture cultivation; The subculture medium composition is 1/2MS+ GA
30.6mg/L+6-BA 0.5mg/L+IAA 0.15mg/L+ silver nitrate 2.0mg/L+3% sucrose; 23 ± 2 ℃ of cultivation temperature, light application time 16h/d, Medium's PH Value 5.8～6.0, cultivate 20 days, forms the long budling with 3 stipes of 4cm, is cut into the segment with stipes, and each budling is divided into 2 sections; Every 20 days subcultures like this once, expand numerous test-tube plantlet;
Step (5) culture of rootage: step (4) is expanded to numerous test-tube plantlet and take out, move into and be equipped with in the closed container of root media, carry out culture of rootage, the culture of rootage based component is MS+ GA
30.5mg/L+IBA 0.5mg/L+6-BA 0.01mg/L+10% sucrose; Test-tube plantlet intensity of illumination in root media is 3000Lux, and 25 ± 2 ℃ of cultivation temperature, be cultured to 30 days, bottle outlet when test-tube plantlet root in medium grows to the new root of 0.5cm;
Step (6) hardening: select healthy and strong test-tube plantlet bottle outlet to be transplanted to vermiculite: in the matrix of the peat composed of rotten mosses=3:1, hardening 40-45 days, air humidity 85-90%, illumination 10000lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100867690A CN102599062B (en) | 2012-03-29 | 2012-03-29 | Method for rapid propagating tissue of wistaria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100867690A CN102599062B (en) | 2012-03-29 | 2012-03-29 | Method for rapid propagating tissue of wistaria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102599062A CN102599062A (en) | 2012-07-25 |
| CN102599062B true CN102599062B (en) | 2013-12-04 |
Family
ID=46517145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100867690A Expired - Fee Related CN102599062B (en) | 2012-03-29 | 2012-03-29 | Method for rapid propagating tissue of wistaria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102599062B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371040A (en) * | 2016-11-21 | 2018-08-07 | 四川七彩林业开发有限公司 | A kind of Chinese wistaria seed type of seeding |
| CN108668905A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method that Chinese wistaria stem section is taken root |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1899027A (en) * | 2002-11-04 | 2007-01-24 | 杨凌润地生态科技有限公司 | Tissue culture quick breeding seedling producing method for kudzu vine |
| CN100553432C (en) * | 2006-11-16 | 2009-10-28 | 杨伟仁 | A kind of breeding method of flower vine grafted landscape |
| CN101176427A (en) * | 2007-12-06 | 2008-05-14 | 中国科学院东北地理与农业生态研究所 | Method for regenerating plant strain using soybean cotyledonary node |
| USPP21981P2 (en) * | 2009-10-13 | 2011-06-21 | Angelo Giorgerini | Wisteria plant named ‘Angie’ |
-
2012
- 2012-03-29 CN CN2012100867690A patent/CN102599062B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102599062A (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104782486B (en) | Tissue culture and intermediate propagation method for succulent Haworthia cooperivar. pilfera M. B. Bayer | |
| CN104041412B (en) | The quick breeding method for tissue culture of a kind of Guizhou half capsule lettuce tongue | |
| CN103931497B (en) | A kind of method improving dragon fruit plantlet in vitro planting percent | |
| CN105475130A (en) | Castanopsis hystrix high efficiency isolated culture plant regeneration method | |
| CN103392597B (en) | A kind of method for tissue culture of North American begonia | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN104585037B (en) | A kind of bottle orchid quick breeding method for tissue culture | |
| CN102845313A (en) | Method for quickly in-vitro actinidia kolomikta propagating | |
| CN104996301A (en) | Rapid propagation method for holcoglossum flavescens | |
| CN103004604B (en) | Breeding method for vanda | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN105165373A (en) | Cutting seedling raising method of Davidia involucrata Baill | |
| CN103460971A (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN105918129B (en) | A kind of tissue culture and rapid propagation method of moonlight jujube | |
| CN107711514B (en) | Tissue culture and rapid propagation method for excellent individual plant of hibiscus purpureus in dry land | |
| CN104542284A (en) | Tissue culture rapid propagation method for rhododendron irroratum | |
| CN103583360A (en) | Method for improving Abelia seedling salt tolerance by oriented induction | |
| CN104221860B (en) | The quick breeding method for tissue culture of a kind of Japanses beauty-berry | |
| CN102599062B (en) | Method for rapid propagating tissue of wistaria | |
| CN106171996B (en) | A kind of rapid propagation method of wild white birch | |
| CN110663552B (en) | Tissue culture and rapid propagation method of Yunnan tung tree | |
| CN105494097A (en) | In-vitro rapid propagation technology of viburnum sargentii koehne | |
| CN105660391A (en) | Tissue culture breeding method for apple sapling | |
| CN105532398B (en) | A kind of hosta tissue culture seedling Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method | |
| CN112042540A (en) | Rapid propagation method of hydrangea macrophylla |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: TAICANG LINJIANG FARM SPECIALIZED COOPERATIVES Free format text: FORMER OWNER: CHANGSHU HAIYU TEA CO., LTD. Effective date: 20131023 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 215522 SUZHOU, JIANGSU PROVINCE TO: 215400 SUZHOU, JIANGSU PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20131023 Address after: 215400, Jiangsu, Taicang Province Jing Huang Zhen Dang village Applicant after: Taicang Linjiang Farm Specialized Cooperatives Address before: 215522 Fushan, Changshou City, Jiangsu, China Applicant before: Changshu Haiyu Tea Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131204 Termination date: 20170329 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |