CN102590482A - Method for identifying male silk of mulberry silkworm - Google Patents

Abstract

The invention discloses a method for identifying male silk of mulberry silkworm, and belongs to the technical field of silk production of textile engineering. The method comprises the following steps of: degumming tested silk to obtain pure silk fibroin and preparing into a powdery sample; testing content values of three amino acids, i.e., tyrosine, methionine and aspartic acid, in the sample in the unit of milligram/milliliter; obtaining chi-square X2 by using a chi-square test method based on the content values of the three amino acids in the silk fibroin of the standard male silk; and judging the tested silk as the male silk if X2 is less than 4.61 under the condition of significance level alpha being equal to 0.10. The content values of the three amino acids in the silk fibroin of the silk are used as a judgment basis; the chi-square test is adopted for processing; and the method is simple and convenient, has an accurate result and has a positive effect on promotion of high-grade male silk and products thereof on domestic and foreign markets.

Description

A kind of method of differentiating the silkworm male silk

Technical field

The present invention relates to a kind of method of discrimination of silkworm male silk, belong to the throwing technical field of Textile Engineering.

Background technology

In fiber tow production, the male silk cocoon silk of reel silk from cocoons system is compared with female silk, and it is thin that male silk has the size of cocoon filament; Cleanliness is excellent, and it is little that single cocoon fiber number is opened difference, and cohesive force is good; Characteristics such as raw silk grade height, good with the fabric elasticity of its preparation, the silk fabric face is flat very, well-pressed, cunning is glutinous, feel is abundant.At present, the male silk of production commodity inspection 6A level 13/15D specification is used for processing braiding suture, and the male silk of 6A level 20/22D specification is used for producing high-grade wedding gauze kerchief and silk fabrics, and the application of male silk on extension product demonstrates unique high-grade advantage.The key of preparation male silk is that the silk cocoon male and female are differentiated, and CN1515150 discloses a kind of method of the mulberry cocoon property declared, and using wavelength is that the ultraviolet light of 3000～3800nm is the fluoroscopic examination light source, is radiated on the silkworm kind with the fluorescence cocoon color property declared characteristic; Publication number is that the Chinese invention patent of CN102095481A provides a kind of Sex in Silkworm Cocoons automatic discriminating gear and method; Its method that Sex in Silkworm Cocoons is differentiated is to obtain silkworm chrysalis X ray original image; With 5 silkworm chrysalis characteristics of image of area, girth, major axis, minor axis, mean flow rate of silkworm chrysalis image and these 6 characteristic parameters of silk cocoon weight as the Sex in Silkworm Cocoons characteristic parameter; According to the artificial cognition model of setting up in advance; Set up the discrimination model of Sex in Silkworm Cocoons characteristic parameter and Sex in Silkworm Cocoons with K-mean cluster method, model is imported to the Sex in Silkworm Cocoons discriminant software sex of silk cocoon is differentiated.

Yet prior art only can realize the property declared to silk cocoon, and how silk is judged, does not also have a kind of method of differentiating male silk at present.

Summary of the invention

The objective of the invention is to overcome the deficiency that prior art exists, provide a kind of simple, convenient, the result differentiates the method for male mulberry silk accurately.

The technical scheme that the present invention adopts provides a kind of method of differentiating the silkworm male silk, the step below adopting:

A kind of method of differentiating the silkworm male silk is characterized in that comprising the steps:

(1) with the processing of coming unstuck of tested silk, obtains pure fibroin, be prepared into pulverized specimen;

(2) measure the content value of methionine in the sample, tyrosine and asparatate three seed amino acids, value unit is the millimicro grams per milliliter;

(3) value with this three seed amino acids content of standard male silk fibroin is a benchmark, adopts the Chi-square Test method, obtains card side X ²

(4) under the condition of level of significance α=0.10, if X ²＜4.61, judge that then silk to be detected is male silk.

Principle of the present invention is that the principal ingredient of silk is made up of fibroin, silk gum etc.Fibroin accounts for 70～80% in the silk, and silk gum accounts for 20～30%, also has a spot of wax material, carbohydrates, pigment, inorganics etc. in addition, and major part is distributed in the silk gum layer.The available part of silk is a fibroin, and silk gum mostly will be sloughed in silk product.There is utmost point significant difference in each amino acid constituent ratio between the male and female natural silk.The principal ingredient content of fibroin is glycocoll, alanine, serine, tyrosine, valine, Tianmen propylhomoserin, glutamic acid, threonine, phenylalanine, isoleucine, arginine, leucine, proline, lysine, histidine, methionine etc. from high to low successively, and each amino acid constituent ratio exists significant difference to be because the difference of methionine, tyrosine, asparatate three's constituent ratio causes between the male and female natural silk.The difference that each amino acid constituent ratio exists between the common natural silk that male and female are mixed and its male silk fibroin is because the difference of methionine, tyrosine, asparatate three's constituent ratio causes equally.The present invention adopts this three seed amino acids content constituent ratio of natural silk to carry out X ²Check analysis just can be differentiated the whether true and false of male silk of sample silk easily.

Compared with prior art; The present invention is the property declared foundation with the content value of methionine, tyrosine and asparatate three seed amino acids in the natural silk; The employing Chi-square Test is handled; Method is simple and convenient, and the result is accurate, for positive effect has been brought into play in high-quality male silk and products thereof popularization on the market at home and abroad.

Embodiment

Below in conjunction with embodiment the present invention is further described.

Embodiment 1

In the present embodiment, it is some to get the fluorescence cocoon color property declared silkworm kind " 101 * 102 " silk cocoon, tells the female cocoon of purple iridescent, the male cocoon of yellowish iridescent with fluorescence detector, and with 80 ℃ of air dry ovens, 20h oven dry, packing is subsequent use.

Get the 25g silk cocoon, be cut into the fragment of 10mm*10mm, in the 1000ml beaker, add the sodium carbonate liquor of 500ml 0.5% with scissors; Boil 45min, pull silk out, after rinsing well with tap water; Place the 1000ml beaker, add 0.5% neutral soap 500ml, 30min again comes unstuck under 100 ℃ of temperature; After water is rinsed well, more once with deionized water rinsing.50 ℃ of drying in oven obtain to come unstuck fibroin.It is 50% calcium chloride solution that the fibroin that will come unstuck is put into 250ml concentration, is heated to boiling with the 500ml beaker, stirs, and fibroin is fully dissolved.After the dissolving fully, cooling, filter, filtrating is packed in the bag filter, dashes to drench with tap water and analyses 3d, uses deionized water dialysis 6h then instead, makes silk fibroin solution.

Get a certain amount of silk fibroin solution in eggplant shape beaker, place deep freezer, make the silk fibroin solution frozen.Change in the freeze-dryer 6 days then over to, obtain the dehydrate of white.Take out,, obtain the fibroin powder of white through grinding.

The male and female silk cocoon fibroin sample of above acquisition is carried out the aminoacid ingredient detection, and amino acid content is a unit with the millimicro grams per milliliter, and its male and female natural silk amino acid is formed referring to table 1.

Table 1

Referring to table 1, related data is made X ²Check (Chi-square Test) is analyzed, and each amino acid content changes between the male and female fibroin, in level of significance α=0.10, and α=0.05 time X ²Check, it is worth X ²=395.23** has reached the utmost point level of signifiance.Explaining that each amino acid constituent ratio differs between the male and female natural silk exists utmost point significant difference.Further each amino acid difference between the male and female natural silk is carried out in level of signifiance α=0.05, under α=0.01 condition, adopt the t check analysis, the result is referring to table 2.

Table 2

The t check analysis is learnt, the content difference of male and female natural silk m-Tyrosine, methionine in α=0.01 time, reaches the utmost point level of signifiance, though other is variant, in α=0.05 time, does not all reach level of significance.Three further bigger to t value between male and female natural silk seed amino acids: the constituent ratio of tyrosine, methionine, asparatate, in level of significance α=0.10, X time carries out in α=0.05 ²Check.X ²=211.72** explains that each amino acid constituent ratio utmost point significant difference between the male and female natural silk is that difference mainly due to tyrosine, methionine, asparatate three's constituent ratio causes.Simultaneously, this three seed amino acid of the mean value of male and female natural silk tyrosine, methionine, asparatate and male silk fibroin carries out X in the his-and-hers watches ²Check analysis, X ²=67.28** (X ² _{0.10 (2)}=4.61), explain that male and female mix the constituent ratio of this three seed amino acid in constituent ratio and its male silk fibroin of tyrosine, methionine, asparatate in the natural silk and have significant difference equally.

Embodiment 2

In the present embodiment; Get silk cocoon fibroin sample that 9 male cocoon shells of 1 female cocoon shell

are mixed with and carry out aminoacid ingredient and detect, the composition of tyrosine, methionine, asparatate is referring to table 3 in its natural silk.

Table 3

Referring to table 3, under level of significance α=0.10 condition, data are made X ²Check analysis, X ²=5.25* greater than 4.61, has reached the significant difference level.

Present embodiment proves: tyrosine, methionine, the asparatate three seed amino acid constituent ratios of the natural silk of the mixing cocoon of forming by 10% female cocoon 90% male cocoon and the natural silk of male cocoon, and under the condition of level of signifiance α=0.10, through its X ²Value compares with 4.61, can judge accurately whether silk to be detected is the true and false of male silk.

Claims (1)

1. a method of differentiating the silkworm male silk is characterized in that comprising the steps:

(1) with the processing of coming unstuck of tested silk, obtains pure fibroin, be prepared into pulverized specimen;

(2) measure the content value of tyrosine in the sample, methionine and asparatate three seed amino acids, value unit is the millimicro grams per milliliter;

(3) value with this three seed amino acids content of standard male silk fibroin is a benchmark, adopts the Chi-square Test method, obtains card side X ²

(4) under the condition of level of significance α=0.10, if X ²＜4.61, judge that then silk to be detected is male silk.