CN102580151B

CN102580151B - External can the processing method of autoplastic in vitro skull sheet

Info

Publication number: CN102580151B
Application number: CN201110438640.7A
Authority: CN
Inventors: 李涛; 付留俊; 郑凤勤
Original assignee: First Affiliated Hospital of Henan University of Science and Technology
Current assignee: First Affiliated Hospital of Henan University of Science and Technology
Priority date: 2011-12-25
Filing date: 2011-12-25
Publication date: 2016-01-20
Anticipated expiration: 2031-12-25
Also published as: CN102580151A

Abstract

The present invention relates to technical field of biological material, the processing method of the bone material particularly in transplantation body, <b> passes through: </b>1. fresh bone lobe is freezing 24 hours at-10 DEG C, under natural environment (? about 20 DEG C) rewarming 48 hours objects allow cell integrity destroy, and fully makes aqtocytolysis; 2. the manual soft tissue removing skull surface; 3. the netted boring of skull, makes area about 1 ㎝ × 1 ㎝ between hole; 4. distilled water adds trypsin and soaks 48 hours in 37 DEG C of environment, and within every 24 hours, change first water, object makes fully to separate out from solution; The medical hydrogen peroxide dipping of <b>5.</bGreatT.G reaT.GT 72 hours, changes hydrogen peroxide once in every 24 hours, fully removes the protein in diploe; Distilled water immersion 12 hours, drains; There is not the untoward reaction such as infection, hydrops.Easily movable for ankle joint, cause foot syringe needle movability, the design is in conjunction with the special construction of foot, and fixing foot, prevents medicinal liquid from infiltrating subcutaneous tissue.

Description

External can the processing method of autoplastic in vitro skull sheet

Technical field

The present invention relates to technical field of biological material, the processing method of the bone material particularly in transplantation body.

Background technology

Neurosurgery is save the life of patient often to take to remove patient's Cranial-bone-flap, reduces patient's intracranial pressure, remove the object of cerebral hernia state to reach, row cranioplasty surgery again after stable disease.Patching material conventional at present adopts artificial material (as titanium alloy reticulated) and autologous bone lobe substantially.Artificial material because it is moulding, withstand voltage, resistance, thermal insulation, rejection, the aspect such as expensive (every tool patching material about more than 20,000 yuan) deficiency, limit it and promote the use of.That other patching materials institute is unapproachable by the advantage that Preserve selef-cranium is repaired, the physical characteristic of Cranial-bone-flap meets the anatomical physiology needs of human body completely, bone-inducting active is good, comparatively speaking, it is ideal patching material, but the preparation that can meet the Autologous skull flap patching material of clinical practice exists larger technology barrier, limit its application; The store method of Autologous skull flap to have in body or external two kinds.Preserve weak point in body and be that its wound is large, bone lobe Absorbable rod diminishes, osteoporosis, and bone lobe buries that position is often infected, ulcer, erosion etc. cause clinical practice unsuccessfully; Storage in vitro method has liquid nitrogen cryogenics coldly to deposit, the method such as high-temperature sterilization and irradiation sterilization; Liquid nitrogen cryogenics is cold exists apparatus expensive, easily pollutes in operating process, and after Hui Zhi, human body is to problems such as the immunoreation of remaining organic substance, not easily promotes; After high-temperature sterilization, the intensity of skull significantly declines, and can not meet clinical needs completely; The method of irradiation sterilization needs special industrialization irradiation apparatus, and hospital is difficult to configuration, and after can not improving skull sheet Hui Zhi, human body is to the immunoreation problem of remaining organic substance.How to prepare meet repairing operation aseptic, have the requirement of sufficient intensity, reduced immunogenicity and can be difficult problem at the autogenous bone flap of most hospital application always.

Summary of the invention

In order to overcome above-mentioned defect, the invention provides a kind of external can the processing method of autoplastic in vitro skull sheet, this foot antiseepage needle fixation device, efficiently solves child's foot and pierces through the difficult problem of position management, improve safety and the comfortableness of patient.

For achieving the above object, the present invention adopts following technical scheme:

We intend by carrying out corresponding technical finesse to fresh Cranial-bone-flap, make it to become to be easy to preserve, and profile compatibility is good, and physical property meets the transplanting patching material of physiological requirement completely, is applied to clinical.

For realizing the object solved the problems of the technologies described above, present invention employs following technical scheme:

A kind of external can the processing method of autoplastic in vitro skull sheet : pass through:

1 fresh bone lobe is freezing 24 hours at-10 DEG C, and under natural environment, (about 20 DEG C) rewarming 48 hours objects allow cell integrity destroy, and fully makes aqtocytolysis;

The 2 manual soft tissues removing skull surface;

The netted boring of 3 skull, makes area about 1 ㎝ × 1 ㎝ between hole;

4 distilled water add trypsin and soak 48 hours in 37 DEG C of environment, and within every 24 hours, change first water, object makes fully to separate out from solution;

5medical hydrogen peroxide dipping 72 hours, changes hydrogen peroxide once in every 24 hours, fully removes the protein in diploe; Distilled water immersion 12 hours, drains;

Defat 4 hours under 6 use chloroform+methanol room temperatures, distilled water is rinsing repeatedly.Clean gauze 4 layers of parcel are placed in 50 DEG C of baking boxs, dry 12 hours, and object separates out moisture and lipid, is sealed in plastic bag for subsequent use;

7 before cranioplasty surgery 2 weeks, the osteocomma that will dry with after 4 layers of medical clean gauzes parcel, used 50 DEG C of oven cooking cycle 12 hours again again;

After 8 encapsulation, 60 DEG C, oxirane sterilization, 24 hours (decontaminating apparatus is 3M8XL type), saves backup under usual terms.

Owing to adopting technical scheme as above, the present invention has following superiority:

A kind of external can the processing method of autoplastic in vitro skull sheet, skull sheet 36 is made by experiment in 2006 ~ 2007 years, 31 routine people are had to apply the capable cranioplasty surgery of Autogenous craniotomy bone flap of processing, all patient's profiles are good, postoperatively in natural environment, do not tell the malaise symptoms such as headache, do not occur the untoward reaction such as infection, hydrops.Easily movable for ankle joint, cause foot syringe needle movability, the design is in conjunction with the special construction of foot, and fixing foot, prevents medicinal liquid from infiltrating subcutaneous tissue.

Accompanying drawing explanation

figure1 is skull bone lobe schematic diagram of the present invention;

fig. 2it is the netted boring schematic diagram of skull of the present invention;

Detailed description of the invention

Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:

Can explain the present invention in more detail by the following examples, disclose object of the present invention and be intended to protect all changes and improvements in the scope of the invention, the present invention is not limited to the following examples;

After fresh bone lobe takes out under clean environment, to be positioned in refrigerator under-10 DEG C of environment freezing 24 hours, (about 20 DEG C) rewarming 48 hours under natural environment; Histiocyte gradually cooling and rewarming process in cell is fully broken, aqtocytolysis.Hairbrush brushes away except residual periosteum, muscle and the fascia tissue of skull surface, and flowing water fully rinses.

With the drill bit of diameter 4mm by netted for skull boring, make area about 1 ㎝ × 1 ㎝ between hole, leave sutura during boring as far as possible, thicker part can suitable reduction holes spacing, and the suitable enlarged hole spacing in thin place, in case skull sheet splits when holing and increases skull intensity.

It is that every 500ml adds 20ku trypsin that distilled water adds trypsin concentration) soak 48 hours in constant temperature 37 DEG C of environment, within every 24 hours, change first water, broken cell component is degraded further, be more conducive to separate out.In hermetic container, with medical hydrogen peroxide, the skull sheet of previous step process is soaked 72 hours, within every 24 hours, change hydrogen peroxide once, fully remove the protein in diploe; Again use distilled water immersion 12 hours, drain under clean environment.

With defat under 1:1 chloroform methanol room temperature 4 hours, distilled water is rinsing repeatedly.Clean gauze 4 layers of parcel are placed in 50 DEG C of baking ovens, dry 12 hours, stir twice in drying course, and the moisture in osteocomma and lipid and remaining solvent are separated out.Be sealed in plastic bag for subsequent use after oven dry.Before cranioplasty surgery 2 weeks, the osteocomma that will dry again with after 4 layers of medical clean gauzes parcel, was again used 50 DEG C of oven cooking cycle 12 hours, is fully separated out moisture, lipid, residual solvent, in order to avoid affect Disinfection Effect.

With oxirane disinfection envelope encapsulation osteocomma and sterilization bar, 60 DEG C, oxirane sterilization 24 hours, checks bar variable color, illustrates that sterilization is qualified, again checks that envelope is without after breakage, saves backup under being placed in usual terms.

Above-described embodiment, the just one of the specific embodiment of the present invention, the usual change that those skilled in the art carries out within the scope of technical solution of the present invention and replacement all should be included within protection scope of the present invention.

It is prior art that the present invention does not carefully state part, is not therefore described further.

Claims

1. one kind external can the processing method of autoplastic in vitro skull sheet : pass through:

(1) fresh bone lobe is freezing 24 hours at-10 DEG C, rewarming 48 hours at natural environment 20 DEG C, and object allows cell integrity destroy, and fully makes aqtocytolysis;

(2) the manual soft tissue removing skull surface;

(3) the netted boring of skull, makes area 1 ㎝ × 1 ㎝ between hole;

(4) distilled water adds trypsin and soaks 48 hours in 37 DEG C of environment, and within every 24 hours, change first water, object makes fully to separate out from solution;

(5)medical hydrogen peroxide dipping 72 hours, changes hydrogen peroxide once in every 24 hours, fully removes the protein in diploe; Distilled water immersion 12 hours, drains;

(6) with defat under chloroform+methanol room temperature 4 hours, distilled water is rinsing repeatedly; Clean gauze 4 layers of parcel are placed in 50 DEG C of baking boxs, dry 12 hours, and object separates out moisture and lipid, is sealed in plastic bag for subsequent use;

(7) before cranioplasty surgery 2 weeks, the osteocomma that will dry with after 4 layers of medical clean gauzes parcel, used 50 DEG C of oven cooking cycle 12 hours again again;

(8) after encapsulation, 60 DEG C, oxirane is sterilized 24 hours, and decontaminating apparatus is 3M8XL type, saves backup under usual terms.