CN102580151A - Method for treating externally-autoplastic autogenous skull sheet - Google Patents
Method for treating externally-autoplastic autogenous skull sheet Download PDFInfo
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- CN102580151A CN102580151A CN2011104386407A CN201110438640A CN102580151A CN 102580151 A CN102580151 A CN 102580151A CN 2011104386407 A CN2011104386407 A CN 2011104386407A CN 201110438640 A CN201110438640 A CN 201110438640A CN 102580151 A CN102580151 A CN 102580151A
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Abstract
The invention relates to the field of biology materials, in particular to a method for treating an externally-autoplastic autogenous skull sheet, which includes: 1 freezing fresh skull flap for 24 hours at temperature of -10 DEG C, rewarming for 48 hours in natural environment (about 20 DEG C) so as to break integrity of cells and lead the cells to be self-dissolved fully; 2 removing soft tissue on the surface of skull manually; 3 drilling holes on the skull in mesh mode and leading areas among holes to be 1cm*1cm; 4 soaking distilled water and trypsin for 48 hours at the temperature of 37 DEG C, changing the distilled water every 24 hours so as to fully precipitine an autogenous solution; and 5 soaking for 72 hours in medical hydrogen dioxide, changing the hydrogen dioxide every 24 hours to fully remove proteins in diploe; and soaking for 12 hours in the distilled water and then draining when no adverse reactions including infection, effusion and the like appear. Aiming at the problem that ankle joints are movable so that needle heads on the foot portion can move easily, the method combines special structure of the foot portion for fixing the foot portion so as to prevent liquid medicine from permeating into subcutaneous tissues.
Description
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Technical field
The present invention relates to technical field of biological material, particularly the processing method of the intravital bone material of transplantation.
Background technology
Neurosurgery often takes to remove patient's Cranial-bone-flap for saving patient's life, reduces patient's intracranial pressure to reach, and removes the purpose of cerebral hernia state, treats to go after the stable disease cranioplasty surgery again.Patching material at present commonly used adopts artificial material (like titanium alloy reticulated) and substantially from body bone lobe.Artificial material limits it and promotes the use of because of the deficiency of aspects such as it is moulding, withstand voltage, resistance, thermal insulation, rejection, cost an arm and a leg (surplus every tool patching material about 20,000 yuan).Using the advantage from the body skull repairing is that other patching material institutes are unapproachable; The physical characteristic of Cranial-bone-flap meets the anatomical physiology needs of human body fully; Bone-inducting active is good, comparatively speaking, is ideal patching material; But there is bigger technology barrier in the preparation from body Cranial-bone-flap patching material that can satisfy clinical practice, has limited its application; Have in the body or external two kinds from the store method of body Cranial-bone-flap.Preserve weak point in the body and be that its wound is big, the bone lobe can absorb and diminish, osteoporosis, and the bone lobe buries that the position is often infected, ulcer, erosion etc. cause the clinical practice failure; External store method has that liquid nitrogen cryogenics is coldly deposited, high-temperature sterilization, and method such as irradiation sterilization; The cold apparatus expensive that exists of liquid nitrogen cryogenics is prone in the operating process pollute, and human body is difficult for promoting to the problems such as immunoreation of remaining organic substance behind the Hui Zhi; The intensity of skull significantly descends after the high-temperature sterilization, can not meet clinical needs fully; The method of irradiation sterilization needs special industrialization irradiation apparatus, and hospital is difficult to configuration, can not improve behind the skull sheet Hui Zhi human body to the immunoreation problem of remaining organic substance.How to prepare satisfy that repairing operation is aseptic, requirement that sufficient intensity, reduced immunogenicity are arranged and can always be a difficult problem at the stripped Cranial-bone-flap of most hospital application.
Summary of the invention
In order to overcome above-mentioned defective, the present invention provides a kind of external processing method that can autoplastic stripped skull sheet, and this foot antiseepage needle fixation device has solved child's foot effectively and pierced through the difficult problem of position management, has improved patient safety and comfortableness.
For realizing the foregoing invention purpose, the present invention adopts following technical scheme:
We intend through fresh Cranial-bone-flap being carried out corresponding techniques and handling, and make it to become to be easy to preserve, and the profile compatibility is good, and the transplanting patching material that the complete accordance with physiology of physical property requires is applied to clinical.
Be the purpose that realizes solving the problems of the technologies described above, the present invention has adopted following technical scheme:
A kind of external processing method that can autoplastic stripped skull sheet
: through:
1 fresh bone lobe-10 ℃ freezing 24 hours, 48 hours purposes of (about 20 ℃) rewarming are to let the cell integrity destroy under the natural environment, fully make aqtocytolysis;
The 2 manual soft tissues of removing skull surface;
The netted boring of 3 skulls makes about 1 ㎝ of area * 1 ㎝ between the hole;
4 distilled water add trypsin and in 37 ℃ of environment, soaked 48 hours, change first water in per 24 hours, and purpose is to make from solution fully to separate out;
5Medical hydrogen peroxide dipping 72 hours was changed hydrogen peroxide once in per 24 hours, fully removed the protein in the diploe; Distilled water immersion 12 hours drains;
Defat is 4 hours under 6 usefulness chloroform+methanol room temperature, and distilled water is rinsing repeatedly.4 layers of parcel of clean gauze are placed in 50 ℃ of baking boxs, dry 12 hours, and purpose is separated out moisture and lipid, is sealed in the plastic bag subsequent use;
7 before cranioplasty surgery 2 weeks, the osteocomma that will dry with behind 4 layers of medical clean gauzes parcel, toasted 12 hours with 50 ℃ of baking boxs again once more;
24 hours (decontaminating apparatus is a 3M 8XL type) of 8 60 ℃ of sterilizations of encapsulation back oxirane, usual terms is preserved subsequent use down.
Because adopt aforesaid technical scheme, the present invention has following superiority:
A kind of external processing method that can autoplastic stripped skull sheet; Made 36 of skull sheets in 2006 ~ 2007 years through experiment; Have 31 routine people use processing from the capable cranioplasty surgery of body skull sheet; All patient's profiles are good, and postoperative is not told malaise symptoms such as headache in natural environment, untoward reaction such as infection, hydrops do not occur.It is movable to be prone to ankle joint, causes foot syringe needle movability, and the design combines the special construction of foot, and fixedly foot prevents that medicinal liquid from infiltrating subcutaneous tissue.
Description of drawings
FigureThe 1st, skull bone lobe sketch map of the present invention;
Fig. 2It is the netted boring sketch map of skull of the present invention;
The specific embodiment
Below in conjunction with the accompanying drawing and the specific embodiment the present invention is done further explain:
Can explain the present invention in more detail through following embodiment, open the object of the invention is intended to protect all variations and the improvement in the scope of the invention, and the present invention is not limited to following embodiment;
After the fresh bone lobe takes out under clean environment, be positioned in the refrigerator under-10 ℃ of environment freezing 24 hours, (about 20 ℃) rewarming is 48 hours under the natural environment; Histiocyte fully breaks cell in the cooling and the process of rewarming gradually, aqtocytolysis.Hairbrush brushes away except that the residual periosteum of skull surface, muscle, reaches fascia tissue, and flowing water fully washes.
With the netted boring of skull, make about 1 ㎝ of area * 1 ㎝ between the hole with the drill bit of diameter 4mm, leave sutura during boring as far as possible, thicker part can suitably reduce pitch of holes, and thin place suitably increases pitch of holes, in case the skull sheet splits when boring and increases skull intensity.
It is that every 500ml adds the 20ku trypsin that distilled water adds trypsin concentration) in 37 ℃ of environment of constant temperature, soaked 48 hours, changed first water in per 24 hours, broken cell component further is degraded, be more conducive to separate out.In hermetic container, will go up the skull sheet that a step handles and soak 72 hours, change hydrogen peroxide once in per 24 hours, fully remove the protein in the diploe with medical hydrogen peroxide; Used distilled water immersion once more 12 hours, and under clean environment, drained.
With defat under the 1:1 chloroform methanol room temperature 4 hours, distilled water is rinsing repeatedly.4 layers of parcel of clean gauze are placed in 50 ℃ of baking ovens, dry 12 hours, stir twice in the drying course, and moisture and lipid and remaining solvent in the osteocomma are separated out.Be sealed in the plastic bag subsequent use after the oven dry.In 2 weeks before cranioplasty surgery, the osteocomma that will dry with behind 4 layers of medical clean gauze parcel, with 50 ℃ of baking box bakings 12 hours, is fully separated out moisture, lipid, residual solvent, in order to avoid influence Disinfection Effect again once more.
With oxirane disinfection envelope encapsulation osteocomma and sterilization bar, 60 ℃ of sterilizations of oxirane 24 hours, the variable color of inspection bar explain that sterilization is qualified, check that once more envelope does not have breakage after, place under the usual terms preservation subsequent use.
Above-described embodiment is a kind of of the specific embodiment of the present invention, and common variation that those skilled in the art carries out in technical scheme scope of the present invention and replacement all should be included within protection scope of the present invention.
The present invention does not carefully state part and is prior art, therefore is not described further.
Claims (1)
1. external processing method that can autoplastic stripped skull sheet
: through:
(1) the fresh bone lobe-10 ℃ freezing 24 hours, 48 hours purposes of (about 20 ℃) rewarming are to let the cell integrity destroy under the natural environment, fully make aqtocytolysis;
(2) the manual soft tissue of removing skull surface;
(3) the netted boring of skull makes about 1 ㎝ of area * 1 ㎝ between the hole;
(4) distilled water adds trypsin and in 37 ℃ of environment, soaked 48 hours, changes first water in per 24 hours, and purpose is to make from solution fully to separate out;
(5)Medical hydrogen peroxide dipping 72 hours was changed hydrogen peroxide once in per 24 hours, fully removed the protein in the diploe; Distilled water immersion 12 hours drains;
(6) with defat under chloroform+methanol room temperature 4 hours, distilled water is rinsing repeatedly; 4 layers of parcel of clean gauze are placed in 50 ℃ of baking boxs, dry 12 hours, and purpose is separated out moisture and lipid, is sealed in the plastic bag subsequent use;
(7) 2 weeks before cranioplasty surgery, the osteocomma that will dry is again with behind 4 layers of medical clean gauze parcel, once more with 50 ℃ of baking box bakings 12 hours;
(8) 24 hours (decontaminating apparatus is a 3M 8XL type) of 60 ℃ of sterilizations of encapsulation back oxirane, usual terms is preserved subsequent use down.
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CN201110438640.7A CN102580151B (en) | 2011-12-25 | 2011-12-25 | External can the processing method of autoplastic in vitro skull sheet |
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CN201110438640.7A CN102580151B (en) | 2011-12-25 | 2011-12-25 | External can the processing method of autoplastic in vitro skull sheet |
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CN102580151A true CN102580151A (en) | 2012-07-18 |
CN102580151B CN102580151B (en) | 2016-01-20 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109874311A (en) * | 2018-03-20 | 2019-06-11 | 姜国 | A kind of skull reduction connector |
CN113750293A (en) * | 2021-10-15 | 2021-12-07 | 青岛蓝皓生物技术有限公司 | Preparation method of bone repair material |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1188015A (en) * | 1996-12-09 | 1998-07-22 | 中国人民解放军第四军医大学第一附属医院 | Lump type defatted antigen-removing heterogenic bone grafting material and preparing method thereof |
US20070248638A1 (en) * | 2006-04-21 | 2007-10-25 | Van Dyke Mark E | Structurally modified acellular tissue engineering scaffolds and methods of production |
CN101172165A (en) * | 2007-11-16 | 2008-05-07 | 广东冠昊生物科技有限公司 | Biological bone renovating material |
-
2011
- 2011-12-25 CN CN201110438640.7A patent/CN102580151B/en not_active Expired - Fee Related
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1188015A (en) * | 1996-12-09 | 1998-07-22 | 中国人民解放军第四军医大学第一附属医院 | Lump type defatted antigen-removing heterogenic bone grafting material and preparing method thereof |
US20070248638A1 (en) * | 2006-04-21 | 2007-10-25 | Van Dyke Mark E | Structurally modified acellular tissue engineering scaffolds and methods of production |
CN101172165A (en) * | 2007-11-16 | 2008-05-07 | 广东冠昊生物科技有限公司 | Biological bone renovating material |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109874311A (en) * | 2018-03-20 | 2019-06-11 | 姜国 | A kind of skull reduction connector |
CN113750293A (en) * | 2021-10-15 | 2021-12-07 | 青岛蓝皓生物技术有限公司 | Preparation method of bone repair material |
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Inventor after: Li Tao Inventor after: Fu Liujun Inventor after: Zheng Fengqin Inventor before: Li Tao Inventor before: Zheng Fengqin Inventor before: Fu Liujun |
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