CN102559777A - Method for preprocessing non-grain biomass - Google Patents
Method for preprocessing non-grain biomass Download PDFInfo
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- CN102559777A CN102559777A CN201210014663XA CN201210014663A CN102559777A CN 102559777 A CN102559777 A CN 102559777A CN 201210014663X A CN201210014663X A CN 201210014663XA CN 201210014663 A CN201210014663 A CN 201210014663A CN 102559777 A CN102559777 A CN 102559777A
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- cellulase
- biomass
- grinding
- freezing
- grain
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention relates to a method for preprocessing a non-grain biomass. The cellulase is added in processes of freezing and grinding, so that the second combination of lignin and cellulose can be reduced, the acting effect of the cellulase can be improved, and toxic substances for inhibiting the growth of alcohol fermentation microorganisms cannot be produced. The method comprises the following steps of: (1) removing foreign substances from the biomass, cleaning and grinding into powders; (2) adding clear water, boiling for sterilizing, fully absorbing and artificially or naturally freezing; (3) grinding the frozen biomass, adding the cellulase, and adjusting the pH and the solid-to-liquid ratio; (4) continuously grinding; (5) insulating and continuously enzymatically hydrolyzing until the activity of the cellulase disappears; and (6) filtering, wherein the filter liquor is applied to alcohol fermentation and the filter residue is the original lignin.
Description
One, technical field:
The present invention relates to a kind of pretreatment process of non-grain biolobic material.
Two, background technology:
The technical bottleneck that faces at present is to lack a kind of low power consuming, pollution-free and pretreatment process efficiently, and s-generation alcohol fuel utilizes the biomass of non-grain classes such as stalk, timber and weeds to produce alcohol fuel.
Xylogen in the biomass, Mierocrystalline cellulose and semicellulose exist with the cocrystallization structure; Cellulase is difficult to directly and Mierocrystalline cellulose combines; Therefore adopt acid system, alkaline process and the quick-fried method of vapour etc. to carry out pre-treatment, these preconditioning techniques can produce the toxic substance that suppresses ethanol fermentation.Freeze thawing is a preconditioning technique that can not produce the toxic substance that suppresses ethanol fermentation, but the pre-treatment of simple freeze thawing still has the leeway that makes full use of again.
Can not produce the toxic substance that suppresses the ethanol fermentation mikrobe with the cellulase degradation Mierocrystalline cellulose; But cellulase activity is low; Transformation period is short, and making the time spent cellulase must directly combine with Mierocrystalline cellulose, and cellulase and Mierocrystalline cellulose combination need to consume the regular hour in the cellulase transformation period.
The time of common adding cellulase is after pre-treatment; Add cellulase after comprising the biomass freeze thawing; There are three problems in such way: the one, after the pre-treatment of above-mentioned generation toxic substance, directly add cellulase, and the hydrolyzed solution of acquisition has also just contained the toxic substance that suppresses ethanol fermentation; The 2nd, through after the pre-treatment, the xylogen, Mierocrystalline cellulose and the semicellulose that become flexible each other, separate have the tendency of the state that turns back to original three's copolymerization voluntarily; The 3rd problem is that the cellulase that adds after the pre-treatment like this mainly is to realize that through stirring enzyme molecule and Mierocrystalline cellulose meet and bonded, and this process is slower.
The pre-treatment of high temperature, high pressure and strong acid and strong base mode and add cellulase and can not synchronous reason be, enzyme non-refractory, high pressure and strong acid and strong base add the structure of the destructive enzyme of joining synchronously under HTHP and strong acid and strong base.
When the mechanical jordaning of papermaking was used for the pre-treatment of cellulose fuel ethanol, the cost of defibrination was higher, and is not satisfactory to the actual effect that promotes cellulase hydrolysis.
Under above-mentioned background, need find a kind of method to realize three targets: the firstth, in the pretreated while of biomass, can carry out the combination of Mierocrystalline cellulose and cellulase synchronously, reduce, avoid xylogen and cellulosic secondary bond; The secondth, after pre-treatment, do not produce the toxic substance that suppresses ethanol fermentation; The 3rd is the combination that promotes Mierocrystalline cellulose and cellulase with mechanical external force.In order to realize above-mentioned target, the present invention proposes a kind of biomass pretreatment method that in freezing grinding, adds cellulase.
Three, summary of the invention:
The present invention is in order to solve the weak point in the above-mentioned background technology; A kind of pretreatment process of non-grain biolobic material is provided; When freezing grinding, add cellulase; It can reduce xylogen and cellulosic secondary bond, raising cellulase action effect, does not produce the toxic substance that suppresses the ethanol fermentation microorganism growth.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is: a kind of pretreatment process of non-grain biolobic material is characterized in that may further comprise the steps:
(1) biomass is removed foreign matter, clean, be crushed into Powdered;
(2) artificial or freezing naturally behind adding clear water, boiling sterilization, the imbibition;
(3) freezing biomass are pulverized the back and add cellulase, regulate PH and solid-to-liquid ratio;
(4) continue to grind;
(5) insulation continues enzymolysis, disappears until cellulase activity;
(6) filter, filtrating is used for ethanol fermentation, and filter residue is the log quality.
After pulverizing, described step (3) also can add other hydrocellulose, hemicellulase.
Described biomass are stalk, timber or weeds.
Compared with prior art, the advantage and the effect that have of the present invention is following:
1, there be not high temperature, high pressure and the strong acid and strong base that is unfavorable for cellulase activity during the freezing milled processed;
2, during freezing grinding, the ice that is frozen into has the effect as quartzy sand sample grinding aid, have ruminate moving
The effect that the thing secondary is chewed has promoted the biomass grinding effect, has shortened the time of grinding, and has practiced thrift the grinding cost, has promoted cellulase and cellulosic combination;
3, do not produce the toxic substance that suppresses the yeast ethanol fermentation;
4, with biomass pre-treatment and the timing of cellulase hydrolysis Mierocrystalline cellulose;
5, can obtain the log quality.
Four, embodiment:
A kind of pretreatment process of non-grain biolobic material is characterized in that may further comprise the steps:
(1) biomass is removed foreign matter, clean, be crushed into Powdered;
(2) artificial or freezing naturally behind adding clear water, boiling sterilization, the imbibition;
(3) freezing biomass are pulverized the back and add cellulase, regulate PH and solid-to-liquid ratio;
(4) continue to grind;
(5) insulation continues enzymolysis, disappears until cellulase activity;
(6) filter, filtrating is used for ethanol fermentation, and filter residue is the log quality.
After pulverizing, described step (3) also can add hydrocellulose enzyme, hemicellulase.
Described biomass are stalk, timber or weeds.
PH value in the described step (3) is 4.5, and solid-to-liquid ratio is 1:10.
Principle of the present invention is, the biological qualitative change after freezing crisp firmly pulverized easily and grind.Ice wherein
Piece can play the effect of grinding aid.Biomass after freezing can be offset the high temperature that defibrination produces when defibrination.Cellulase in such defibrination process, can realize fast and Mierocrystalline cellulose meets, combines.Therefore xylogen and cellulosic secondary bond have been avoided.
Biomass at this indication are meant, comprise that stalk, timber, weeds etc. are used for the biomass of s-generation alcohol fuel.The enzyme that during freezing grinding, adds comprises the enzyme of cellulase and other hydrocellulose, semicellulose.
The present invention receives the spontaneous phenomenon enlightenment, and natural ruminating animal carries out secondary to the grass of eating and chews, and is promoting cellulosic separation and Mierocrystalline cellulose fully contacting with cellulase through the physical action of chewing in fact exactly; Chemical-mechanical pulping in the papermaking refining process is that chemical hydrolysis and physical mechanical grinding are combined.
Embodiment 1:
Get wheat stalk, remove and to be ground into behind the foreign matter Powderedly, get its 1kg; Add till boiling tap water sterilization, imbibition to the suction no longer, remove unnecessary free-water, put to be iced under-20 ℃ to form in the cryogenic refrigerator and freeze piece; After carrying out 5-10 minute mechanical jordaning afterwards, remove unnecessary free-water, add cellulase (10-15IU/g) and damping fluid; Adjusting to optimal pH and be 4.5 is to continue behind the 1:10 to grind 5 minutes with solid-to-liquid ratio again, takes out back insulation and continued cellulosic enzymolysis 48 hours under optimum temperuture, filters; Filter residue is used for xylogen to be collected, and filtrating is used for follow-up ethanol fermentation.
Embodiment 2:
Get wheat stalk, the removal foreign matter is ground into Powdered, adds water sterilization, imbibition, removes superfluous water; Put the open air,, remove superfluous water by the freezing back of winter climate condition mechanical disintegration; Add cellulase (10-15IU/g) and damping fluid, adjustment pH be 4.5 with solid-to-liquid ratio be that the 1:10 continued is ground, take out back insulation and continue cellulosic enzymolysis and disappear under optimum temperuture until cellulase activity; Filter, filter residue is used for xylogen to be collected, and filtrating is used for follow-up ethanol fermentation.
Claims (3)
1. the pretreatment process of a non-grain biolobic material is characterized in that may further comprise the steps:
(1) biomass is removed foreign matter, clean, be crushed into Powdered;
(2) artificial or freezing naturally behind adding clear water, boiling sterilization, the imbibition;
(3) freezing biomass are pulverized the back and add cellulase, regulate PH and solid-to-liquid ratio;
(4) continue to grind;
(5) insulation continues enzymolysis, disappears until cellulase activity;
(6) filter, filtrating is used for ethanol fermentation, and filter residue is the log quality.
2. the pretreatment process of a kind of non-grain biolobic material according to claim 1 is characterized in that: also can add other hydrocellulose, hemicellulase after described step (3) is pulverized.
3. the pretreatment process of a kind of non-grain biolobic material according to claim 1 and 2 is characterized in that: described biomass are stalk, timber or weeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201210014663XA CN102559777A (en) | 2012-01-18 | 2012-01-18 | Method for preprocessing non-grain biomass |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210014663XA CN102559777A (en) | 2012-01-18 | 2012-01-18 | Method for preprocessing non-grain biomass |
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| CN102559777A true CN102559777A (en) | 2012-07-11 |
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| CN201210014663XA Pending CN102559777A (en) | 2012-01-18 | 2012-01-18 | Method for preprocessing non-grain biomass |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293862A (en) * | 2014-09-19 | 2015-01-21 | 北京利晟新能生物科技中心（有限合伙） | Method for preventing material in enzymolysis tank from precipitation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875949A (en) * | 2010-07-05 | 2010-11-03 | 中兴能源(内蒙古)有限公司 | Method for producing ethanol fuel by using sweet sorghum stalks |
| WO2011087965A1 (en) * | 2010-01-15 | 2011-07-21 | Xyleco, Inc. | Cooling and processing materials |
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2012
- 2012-01-18 CN CN201210014663XA patent/CN102559777A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011087965A1 (en) * | 2010-01-15 | 2011-07-21 | Xyleco, Inc. | Cooling and processing materials |
| CN101875949A (en) * | 2010-07-05 | 2010-11-03 | 中兴能源(内蒙古)有限公司 | Method for producing ethanol fuel by using sweet sorghum stalks |
Non-Patent Citations (3)
| Title |
|---|
| 《酿酒科技》 20071231 刘海臣等 耐高温酵母利用稻草粉同步糖化发酵的初步研究 摘要、第25页左栏第1-2段，第26页"1.2.2.2、1.2.5、2.2.2" 1-3 第161卷, 第11期 * |
| 刘海臣等: "耐高温酵母利用稻草粉同步糖化发酵的初步研究", 《酿酒科技》, vol. 161, no. 11, 31 December 2007 (2007-12-31) * |
| 孔明惠: "一种高效千冻研磨破碎酵母细胞的方法", 《安徽农业科学》, vol. 38, no. 29, 31 December 2010 (2010-12-31), pages 16124 - 16126 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293862A (en) * | 2014-09-19 | 2015-01-21 | 北京利晟新能生物科技中心（有限合伙） | Method for preventing material in enzymolysis tank from precipitation |
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Application publication date: 20120711 |