CN104293862A - Method for preventing material in enzymolysis tank from precipitation - Google Patents
Method for preventing material in enzymolysis tank from precipitation Download PDFInfo
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- CN104293862A CN104293862A CN201410483809.4A CN201410483809A CN104293862A CN 104293862 A CN104293862 A CN 104293862A CN 201410483809 A CN201410483809 A CN 201410483809A CN 104293862 A CN104293862 A CN 104293862A
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Abstract
The invention provides a method for preventing a material in an enzymolysis tank from precipitation. The hydrolysis slag or material obtained by pretreating a gas explosion material is firstly ground and then sent into the enzymolysis tank, so that the material can enter the enzymolysis tank after becoming paste; and thus, the material can not precipitate after entering the enzymolysis tank, the addition amount of the enzyme preparation is lowered, and the enzymolysis time for the ground material is shortened, The enzymolysis tank does not need any rotary stirring device, thereby lowering the stirring device investment and reducing the power consumption; and thus, the method increases the enzymolysis effect and enhances the work efficiency.
Description
Technical field
The present invention relates to a kind of method preventing material precipitation in enzymatic vessel.
Background technology
Agriculture and forestry organic waste material lignocellulose raw material is used to carry out biorefinery as a kind of emerging biotechnology; meet the needs of China's recycling economy and the strategy of sustainable development, be also alleviating energy crisis, food shortage, alleviate environmental pollution and the Important Action to fossil resources dependency degree.
The main component of the agriculture and forestry organic waste materials such as stalk is Mierocrystalline cellulose, hemicellulose, xylogen.A lot of research institution and producer is had now all to come research and the test works such as production bio-based chemical industry alcohol, bioenergy with agriculture and forestry organic waste materials such as stalks doing, one of them important stage carries out pre-treatment by biomass or by acid hydrolysis or by gas explosion exactly, material pre-treatment obtained again afterwards directly carries out enzymic hydrolysis process, obtains required liquid glucose; Liquid glucose is obtained different products by different fermentable.
Present enzymolysis process, all there is a total problem when carrying out enzymic hydrolysis process is exactly ceaselessly will stir the material of enzymatic vessel, as shown in Figure 1, precipitate for preventing material and will install whipping appts additional on enzymatic vessel, whipping appts ceaselessly will rotate stirring, otherwise discharge pipe blocks in enzymatic vessel as easy as rolling off a log formation precipitation by pretreated material, therefore a whipping appts corresponding with enzymatic vessel size must be installed in enzymatic vessel.The larger required whipping appts of enzymatic vessel is larger, and invest larger, power consumption is also larger; And if have material, whipping appts just can not pause, and break down if stir, the material in enzymatic vessel will precipitate, and causes a whole tank material not show, causing very large waste, carrying out rinsing or falling material as utilized large water gaging, then greatly increase workload and cost.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method preventing material precipitation in enzymatic vessel, pretreated material ground by the method before entering enzymatic vessel, make material become pasty state and enter enzymatic vessel again, not only can not precipitate after entering enzymatic vessel, also can increase hydrolysis result.
For achieving the above object, the present invention by the following technical solutions:
Prevent a method for material precipitation in enzymatic vessel, comprise the following steps:
1) pre-treatment: hydrolysis slag charge or gas explosion material slag charge after squeezing machine is separated, in cellulase: slag charge: the ratio that water is 0.12 ~ 0.28:12 ~ 14:86 ~ 88 according to the ratio of mass fraction enters pre-enzymatic vessel, maintain pre-hydrolysis temperature 48 ~ 52 DEG C, PH4.8 ~ 5.0, pre-enzymolysis 5 ~ 10 hours, becomes " solid-liquid mixture ";
2) grind: " solid-liquid mixture " ground, mesh-of-grind is less than 40 orders;
3) enzymolysis: the pre-enzymolysis material after grinding enters enzymatic vessel enzymolysis.
Further, described step 2) adopt double plate mill to grind.
Further, before grinding, described solid-liquid mixture impeller pump is squeezed into DD type double plate mill.
Further, described step 3) controlled enzymatic hydrolysis tank temperature 48 ~ 52 DEG C, PH4.8 ~ 5.0, acidity≤3, enzymolysis discharging after 40 ~ 60 hours.
Further, described step 3) utilize enzymatic vessel discharging pump every two hours, enzymolysis material to be extracted out at the bottom of tank, self-circulation is carried out at the top being back to tank, each circulation 2 hours.Object is that in tank, material state is even.Two hours just can by the Matter Transfer in tank once, termination of pumping afterwards also can not precipitate for two hours, and plays energy-saving effect.
Further, described step 3) with pump, the material after grinding is squeezed into enzymatic vessel and carried out enzymolysis.
Further, described step 2) adopt DD type double plate mill to grind.
The present invention compared with prior art has the following advantages:
Material grinds by the present invention by the double plate mill of pretreated material by model DD series before entering enzymatic vessel, makes material become pasty state and enters enzymatic vessel again.Solve following problem:
1) need not stir after the material through grinding enters enzymatic vessel and also can not form precipitation;
2) this enzymatic vessel need not install gyratory agitation device additional, decreases the investment of whipping appts, decreases power consumption;
3) attenuate also can increase the osmotic effect of zymin owing to grinding rear material, the same enzyme preparation has been reduced to from the existing addition being greater than 3% and is less than 2%, thus increase hydrolysis result.
4) time that the object after grinding carries out enzymolysis significantly reduced from existing more than 65 hours, increased work efficiency.
5) enzymatic vessel discharging pump was extracted enzymolysis material out every two hours at the bottom of tank, and a self-circulation is carried out at the top being back to tank, makes material state in tank even.Two hours just can by the Matter Transfer in tank once, termination of pumping afterwards also can not precipitate for two hours, and plays energy-saving effect.
Accompanying drawing explanation
Fig. 1 is the artwork of prior art;
Fig. 2 is artwork of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described further:
Embodiment 1
As shown in Figure 2, the present embodiment comprises the following steps:
1, pre-treatment: hydrolysis slag charge or gas explosion material slag charge after squeezing machine is separated, in cellulase: slag charge: water is that 0.12:12:88 ratio adds cellulase, slag charge and water and enters pre-enzymatic vessel according to the ratio of mass fraction, maintain pre-hydrolysis temperature 52 DEG C, PH4.8,10 hours time, become " solid-liquid mixture ";
2, " solid-liquid mixture " enters DD type double plate mill and grinds, and after grinding, material reaches and do not have obvious particulate state and fineness reaches 80 orders is qualified, squeezes into enzymatic vessel and carries out enzymolysis.
3, abrasive enzymolysis: pre-enzymolysis material enters enzymatic vessel, controlled enzymatic hydrolysis tank temperature 52 DEG C, PH5.0, acidity≤3 after grinding, and enzymolysis time, just can discharging after 60 hours.In addition, utilize enzymatic vessel discharging pump to beat every two hours and once circulate, each circulation 2 hours.
Embodiment 2
The present embodiment comprises the following steps:
1, pre-treatment: hydrolysis slag charge or gas explosion material slag charge after squeezing machine is separated, in cellulase: slag charge: water is that 0.28:14:86 ratio adds cellulase, slag charge and water and enters pre-enzymatic vessel according to the ratio of mass fraction, maintain pre-hydrolysis temperature 48 DEG C, PH4.8,5 hours time, become " solid-liquid mixture ";
2, " solid-liquid mixture " enters DD type double plate mill and grinds, and after grinding, material reaches and do not have obvious particulate state and fineness reaches 40 orders is qualified, squeezes into enzymatic vessel and carries out enzymolysis.
3, abrasive enzymolysis: pre-enzymolysis material enters enzymatic vessel, controlled enzymatic hydrolysis tank temperature 48 DEG C, PH4.8, acidity≤3 after grinding, and enzymolysis time, just can discharging after 40 hours.In addition, utilize enzymatic vessel discharging pump to beat every two hours and once circulate, each circulation 2 hours.
Embodiment 3
The present embodiment comprises the following steps:
1, pre-treatment: hydrolysis slag charge or gas explosion material slag charge after squeezing machine is separated, in cellulase: slag charge: water adds cellulase, slag charge and water according to the ratio that the ratio of mass fraction is 0.2:13:87 and enters pre-enzymatic vessel, maintain pre-hydrolysis temperature 50 DEG C, PH4.9,8 hours time, become " solid-liquid mixture ";
2, " solid-liquid mixture " enters DD type double plate mill and grinds, and after grinding, material reaches and do not have obvious particulate state and fineness reaches 60 orders is qualified, squeezes into enzymatic vessel and carries out enzymolysis.
3, abrasive enzymolysis: pre-enzymolysis material enters enzymatic vessel, controlled enzymatic hydrolysis tank temperature 50 C, PH4.9, acidity≤3 after grinding, and enzymolysis time, just can discharging after 50 hours.In addition, utilize enzymatic vessel discharging pump to beat every two hours and once circulate, each circulation 2 hours.
The above embodiment of the present invention is only the good embodiment of this patent, the structure that all employing the technical program describes, feature and the change in its spiritual principle, modifies the protection domain all belonging to this patent.
Claims (6)
1. prevent a method for material precipitation in enzymatic vessel, it is characterized in that: comprise the following steps:
1) pre-treatment: hydrolysis slag charge or gas explosion material slag charge after squeezing machine is separated, in cellulase: slag charge: the ratio that water is 0.12 ~ 0.28:12 ~ 14:86 ~ 88 according to the ratio of mass fraction enters pre-enzymatic vessel, maintain pre-hydrolysis temperature 48 ~ 52 DEG C, PH4.8 ~ 5.0, pre-enzymolysis 5 ~ 10 hours, becomes " solid-liquid mixture ";
2) grind: " solid-liquid mixture " ground, mesh-of-grind is less than 40 orders;
3) enzymolysis: the pre-enzymolysis material after grinding enters enzymatic vessel enzymolysis.
2. the method preventing material precipitation in enzymatic vessel according to claim 1, is characterized in that: described step 2) adopt double plate mill to grind.
3. the method preventing material precipitation in enzymatic vessel according to claim 2, is characterized in that: before grinding, described solid-liquid mixture impeller pump is squeezed into DD type double plate mill.
4. the method preventing material precipitation in enzymatic vessel according to claim 1, is characterized in that: described step 3) controlled enzymatic hydrolysis tank temperature 48 ~ 52 DEG C, PH4.8 ~ 5.0, acidity≤3, enzymolysis discharging after 40 ~ 60 hours.
5. the method preventing material precipitation in enzymatic vessel according to claim 1, it is characterized in that: described step 3) utilize enzymatic vessel discharging pump every two hours, enzymolysis material to be extracted out at the bottom of tank, self-circulation is carried out at the top being back to tank, each circulation 2 hours.
6. the method preventing the precipitation of material in enzymatic vessel according to claim 1, is characterized in that: described step 3) with pump, the material after grinding is squeezed into enzymatic vessel and carry out enzymolysis.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701836A (en) * | 2016-12-06 | 2017-05-24 | 北京利晟新能生物科技中心（有限合伙） | Pretreatment method for preparation of biomass fuels by comprehensive utilization of all components of plant straw |
| CN114195596A (en) * | 2021-12-16 | 2022-03-18 | 山西欣普旺农业科技有限公司 | Aroma-enhancing organic water-soluble fertilizer for sunshine grapes and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390596A (en) * | 2008-10-15 | 2009-03-25 | 福建南海食品有限公司 | Production method of debitterized honey pomelo meal fiber |
| CN101463571A (en) * | 2008-12-08 | 2009-06-24 | 广西科学院 | Pretreatment method for ultra-high pressure blasting wood fiber material |
| CN102559777A (en) * | 2012-01-18 | 2012-07-11 | 陕西科技大学 | Method for preprocessing non-grain biomass |
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2014
- 2014-09-19 CN CN201410483809.4A patent/CN104293862A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390596A (en) * | 2008-10-15 | 2009-03-25 | 福建南海食品有限公司 | Production method of debitterized honey pomelo meal fiber |
| CN101463571A (en) * | 2008-12-08 | 2009-06-24 | 广西科学院 | Pretreatment method for ultra-high pressure blasting wood fiber material |
| CN102559777A (en) * | 2012-01-18 | 2012-07-11 | 陕西科技大学 | Method for preprocessing non-grain biomass |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701836A (en) * | 2016-12-06 | 2017-05-24 | 北京利晟新能生物科技中心（有限合伙） | Pretreatment method for preparation of biomass fuels by comprehensive utilization of all components of plant straw |
| CN114195596A (en) * | 2021-12-16 | 2022-03-18 | 山西欣普旺农业科技有限公司 | Aroma-enhancing organic water-soluble fertilizer for sunshine grapes and preparation method thereof |
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Effective date of registration: 20160218 Address after: 100192, D101A-71, building B-2, Dongsheng Science Park, No. 66 Xiao Dong Road, Beijing, Haidian District, Zhongguancun Applicant after: Lisheng biological science and Technology (Beijing) Co., Ltd. Address before: 100192, D101A-26, building B-2, Dongsheng Science Park, No. 66 Xiao Dong Road, Beijing, Haidian District, Zhongguancun Applicant before: Beijing Lisheng new energy Biotechnology Center (limited partnership) |
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