CN102559531B - Haemophilus parasuis 5 type high-density fermentation medium and application thereof - Google Patents
Haemophilus parasuis 5 type high-density fermentation medium and application thereof Download PDFInfo
- Publication number
- CN102559531B CN102559531B CN201010596059.3A CN201010596059A CN102559531B CN 102559531 B CN102559531 B CN 102559531B CN 201010596059 A CN201010596059 A CN 201010596059A CN 102559531 B CN102559531 B CN 102559531B
- Authority
- CN
- China
- Prior art keywords
- haemophilus parasuis
- substratum
- settled
- dipotassium hydrogen
- mixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention belongs to the technical field of agricultural microbe, and particularly to relates to a Haemophilus parasuis 5 type high-density fermentation medium which includes the following components: sodium glutamate 1 to 10g/L, yeast powder 5 to 50 g/L, sodium chloride 1 to 10 g/L, potassium phosphate 0.063 to 2.502g/L, and sanshui dipotassium hydrogen phosphate 0.351 to 14.044g/L as measured according to weight/volume, and includes bovine serum 5 to 40%, NAD 1 to per mille 10, and water. The Haemophilus parasuis 5 type high-density fermentation medium facilitates the high-density fermentation of the Haemophilus parasuis 5 type, the thallus growth speed is quick, more thallus content can be obtained, and the viable count of the cultivated Haemophilus parasuis 5 type amounts to 5 billion. The fermentation medium has a wide material source, is low in cost, can effectively widen and increase the Haemophilus parasuis 5 type and satisfies large vaccine production.
Description
Technical field
The invention belongs to agriculture microbial technology field, be specifically related to a kind of haemophilus parasuis 5 type high-density fermentation medium and application.
Background technology
Haemophilus parasuis (Haemophilus parasuis, HPs) be conventional a kind of bacterium of being everlasting of raising the swinery upper respiratory tract, but can invade under given conditions body and cause serious systemic disease, take fiber disposition polyserositis, sacroiliitis and meningitis is feature, this disease is called again leather and draws Se Shi sick (Oliverira et al., 2004).The early stage microbiotic that adopts is treated, but this bacterium resistance phenomenon is more and more common, common drug to this disease without obvious curative effect, and, along with people are more and more higher to the expectation of drug residue free, green food demand, the restriction that current many countries apply in animal-feed microbiotic is also more and more stricter, and microbiotic will be subject to strict restriction and progressively forbid in the application of the aspects such as the prevention of this disease and control, therefore, vaccine inoculation oneself become prevention and control this sick major measure.
TSB substratum is as the traditional substratum of HPs, and the basal component of this substratum is: glucose 2.5g/L, casein food grade 17g/L, potassium primary phosphate 2.5g/L, pancreatic juice soy peptone 3g/L.The advantage of this substratum is preparation easily, and is compound substratum.But TSB culture medium cost is higher, the viable count of last gained is less, and general viable count is 15～2,000,000,000, and the production cycle is long.In addition, in TSB substratum, the kind of nitrogen source and content all seldom, are unfavorable for thalline dramatic growth.
Summary of the invention
The object of the invention is to overcome the defect of prior art, the needs of cultivating for haemophilus parasuis 5 type high density fermentations and a kind of high performance fermention medium that screens, this substratum can guarantee that haemophilus parasuis growth is vigorous, carry out eubolism, can improve the speed of growth again, shorten the production time, and this substratum can be adapted to haemophilus parasuis production of vaccine completely.
Substratum prepared by the present invention is a kind of low production cost, and viable count is higher, and general viable count is 45～5,000,000,000, and high density fermentation culture medium with short production cycle.
The present invention realizes by following technical proposal:
Be applicable to a high density fermentation culture medium for haemophilus parasuis 5 types, described substratum prepares in accordance with the following steps:
A, get Sodium Glutamate 1～10g/L, yeast powder 5～50g/L, sodium-chlor 1～10g/L, is settled to 1000ml after dissolving with distilled water, adjusts pH to 7.2～8.0 of substratum, sterilizing 20～30min under the high pressure steam of 115～121 ℃;
B, take the anhydrous potassium dihydrogenphosphate of 27.2g, adding distil water is settled to 1000ml, is mixed with potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1000ml, be mixed with dipotassium hydrogen phosphate solution; Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two is mixed, be mixed with phosphate buffer soln, sterilizing 20～30min under the high pressure steam of 121 ℃;
C, by volume meter, get step B phosphate buffer soln 1～40%, the bovine serum 5～40% of filtration sterilization, Reduced nicotinamide-adenine dinucleotide 1～10 ‰, the substratum that adds sterilized steps A, obtains haemophilus parasuis 5 type high-density fermentation medium.
Substratum provided by the invention is prepared by following method:
A, get Sodium Glutamate 1～10g/L, yeast powder 5～50g/L, sodium-chlor 1～10g/L, is settled to 1000ml after dissolving with distilled water, adjusts pH to 7.2～8.0 of substratum, sterilizing 20～30min under the high pressure steam of 115～121 ℃;
B, take the anhydrous potassium dihydrogenphosphate of 27.2g, adding distil water is settled to 1000ml, is mixed with potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1000ml, be mixed with dipotassium hydrogen phosphate solution; Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two is mixed, be mixed with phosphate buffer soln, sterilizing 20～30min under the high pressure steam of 121 ℃;
C, by volume meter, get step B phosphate buffer soln 1～40%, the bovine serum 5～40% of filtration sterilization, Reduced nicotinamide-adenine dinucleotide 1～10 ‰, the substratum that adds sterilized steps A, obtains haemophilus parasuis 5 type high-density fermentation medium.
Feature of the present invention is: substratum of the present invention using Sodium Glutamate be monosodium glutamate as the main carbon source of haemophilus parasuis 5 types, both reduced production cost, and Sodium Glutamate is as small molecules carbon source material, be more conducive to absorbed and utilize by haemophilus parasuis 5 types.The present invention is usingd yeast powder as major nitrogen source, and yeast powder not only can, for thalline provides nitrogenous source, can also provide necessary various trace elements for thalline as a kind of compound nitrogen source.Phosphate buffered saline buffer on the one hand can be effectively in and the acidic substance that produce in haemophilus parasuis 5 type process of growth, thereby promote growing microorganism.Phosphoric acid salt is the necessary material of the meta-bolitess such as the synthetic ATP of bacterium on the other hand.Therefore this substratum is more more economical and be more conducive to the growth of bacterium than business substratum TSB.
Accompanying drawing explanation
Fig. 1: be the growth curves (contrast) of haemophilus parasuis 5 types in business substratum TSB.
Fig. 2: be the growth curve in the substratum prepared at most preferred embodiment of the present invention of haemophilus parasuis 5 types.
Embodiment
Embodiment 1:
The preparation of substratum:
(1) by Sodium Glutamate 1g, yeast powder 5g, sodium-chlor 1g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.2 of substratum, sterilizing 30min under 115 ℃ of high pressure steam.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate, standby; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution, standby; Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two is mixed, be made into phosphate buffer soln, sterilizing 30min under 115 ℃ of high pressure steam.
(3) when step (1) substratum is cooled to 35 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 1%, the bovine serum 5% (v/v) and the NAD1 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 2: the preparation of Optimal Medium
(1) by Sodium Glutamate 5g, yeast powder 20g, sodium-chlor 4g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.5 of substratum, at 117 ℃ of high pressure steam sterilization 25min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate, standby; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution, standby.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 117 ℃ of high pressure steam sterilization 25min.
(3), when step (1) substratum is cooled to 37 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 20%.The bovine serum 20% (v/v) and the NAD3 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 3: the preparation of Optimal Medium
(1) by Sodium Glutamate 10g, yeast powder 50g, sodium-chlor 10g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.6 of substratum, at 121 ℃ of high pressure steam sterilization 20min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate, standby; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution, standby.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 121 ℃ of high pressure steam sterilization 20min.
(3), when step (1) substratum is cooled to 40 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 40%.The bovine serum 40% (v/v) and the NAD10 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 4: the preparation of Optimal Medium
(1) by Sodium Glutamate 2g, yeast powder 6g, sodium-chlor 2g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.3 of substratum, at 116 ℃ of high pressure steam sterilization 26min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 116 ℃ of high pressure steam sterilization 26min.
(3), when step (1) substratum is cooled to 36 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 2%.The bovine serum 6% (v/v) and the NAD2 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 5: the preparation of Optimal Medium
(1) by Sodium Glutamate 8g, yeast powder 40g, sodium-chlor 8g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.8 of substratum, at 118 ℃ of high pressure steam sterilization 24min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 118 ℃ of high pressure steam sterilization 24min.
(3), when step (1) substratum is cooled to 38 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 30%.The bovine serum 35% (v/v) and the NAD8 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 6: the preparation of Optimal Medium
(1) by Sodium Glutamate 2.5g, yeast powder 30g, sodium-chlor 5g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.4 of substratum, at 121 ℃ of high pressure steam sterilization 20min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 121 ℃ of high pressure steam sterilization 20min.
(3), when step (1) substratum is cooled to 39 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 5%.The bovine serum 10% (v/v) and the NAD2 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Embodiment 7: the preparation of Optimal Medium
(1) by Sodium Glutamate 5g, yeast powder 25g, sodium-chlor 2.5g, after dissolving, is settled to 1L with distilled water, adjusts the pH to 7.4 of substratum, at 121 ℃ of high pressure steam sterilization 20min.
(2) take 27.2g anhydrous potassium dihydrogenphosphate adding distil water and be settled to 1L, be configured to potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1L, be configured to dipotassium hydrogen phosphate solution.Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two mixes, and is made into phosphate buffer solution, in 121 ℃ of high pressure steam sterilization 20min.
(3), when step (1) substratum is cooled to 34 ℃, in sterilized substratum, add described phosphate buffered saline buffer (v/v) 20%.The bovine serum 15% (v/v) and the NAD5 ‰ (v/v) that add filtration sterilization, obtain haemophilus parasuis 5 type high-density fermentation medium again.
Table 1: haemophilus parasuis 5 type 3L fermentor tank experiments
Reference:
[1]Oliveira?S，Pijoan?C.Computer-based?analysis?of?Haemophilus?parasuis?protein?fingerprints.Can?J?Vet?Res，2004a，68：71-75
[2]Oliveira?S，Pijoan?C.Haemophilus?parasuis：new?trends?on?diagnosis，epidemiology?and?contro1.Vet?Microbiol，2004b，99：1-12
Claims (3)
1. be applicable to a high density fermentation culture medium for haemophilus parasuis 5 types, it is characterized in that described substratum prepares in accordance with the following steps:
A, get Sodium Glutamate 1～10g/L, yeast powder 5～50g/L, sodium-chlor 1～10g/L, is settled to 1000ml after dissolving with distilled water, adjusts pH to 7.2～8.0 of substratum, sterilizing 20～30min under the high pressure steam of 115～121 ℃;
B, take the anhydrous potassium dihydrogenphosphate of 27.2g, adding distil water is settled to 1000ml, is mixed with potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1000ml, be mixed with dipotassium hydrogen phosphate solution; Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two is mixed, be mixed with phosphate buffer soln, sterilizing 20～30min under the high pressure steam of 121 ℃;
C, by volume meter, get the phosphate buffer soln 1～40% of step B, the bovine serum 5～40% of filtration sterilization, Reduced nicotinamide-adenine dinucleotide 1～10 ‰, the substratum that adds sterilized steps A, obtains haemophilus parasuis 5 type high-density fermentation medium.
2. a preparation method who is applicable to the high density fermentation culture medium of haemophilus parasuis 5 types, its feature comprises the following steps:
A, get Sodium Glutamate 1～10g/L, yeast powder 5～50g/L, sodium-chlor 1～10g/L, is settled to 1000ml after dissolving with distilled water, adjusts pH to 7.2～8.0 of substratum, sterilizing 20～30min under the high pressure steam of 115～121 ℃;
B, take the anhydrous potassium dihydrogenphosphate of 27.2g, adding distil water is settled to 1000ml, is mixed with potassium dihydrogen phosphate; Take 45.6g tri-water dipotassium hydrogen phosphate adding distil waters and be settled to 1000ml, be mixed with dipotassium hydrogen phosphate solution; Get 23ml potassium dihydrogen phosphate and 77ml dipotassium hydrogen phosphate solution, the two is mixed, be mixed with phosphate buffer soln, sterilizing 20～30min under the high pressure steam of 121 ℃;
C, by volume meter, get the phosphate buffer soln 1～40% of step B, the bovine serum 5～40% of filtration sterilization, Reduced nicotinamide-adenine dinucleotide 1～10 ‰, the substratum that adds sterilized steps A, obtains haemophilus parasuis 5 type high-density fermentation medium.
3. the application of substratum claimed in claim 1 in the high density fermentation of haemophilus parasuis 5 types.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010596059.3A CN102559531B (en) | 2010-12-15 | 2010-12-15 | Haemophilus parasuis 5 type high-density fermentation medium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010596059.3A CN102559531B (en) | 2010-12-15 | 2010-12-15 | Haemophilus parasuis 5 type high-density fermentation medium and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102559531A CN102559531A (en) | 2012-07-11 |
| CN102559531B true CN102559531B (en) | 2014-03-19 |
Family
ID=46406142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010596059.3A Active CN102559531B (en) | 2010-12-15 | 2010-12-15 | Haemophilus parasuis 5 type high-density fermentation medium and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102559531B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232962A (en) * | 2013-05-08 | 2013-08-07 | 青岛农业大学 | High-density fermentation culture medium for haemophilus parasuis and preparation method for same |
| CN103667160B (en) * | 2013-12-30 | 2015-09-23 | 山东滨州博莱威生物技术有限公司 | A kind of haemophilus parasuis high density fermentation culture medium |
| CN106244445A (en) * | 2016-08-17 | 2016-12-21 | 山东滨州博莱威生物技术有限公司 | The continuous fermentation apparatus of a kind of haemophilus parasuis and culture process |
| CN107177531B (en) * | 2017-06-15 | 2018-07-06 | 广东海大畜牧兽医研究院有限公司 | A kind of growth accelerator for improving haemophilus parasuis in vitro culture |
| CN108977392B (en) * | 2018-08-14 | 2021-08-06 | 沈阳农业大学 | Haemophilus parasuis proliferation medium and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1942204A (en) * | 2004-04-26 | 2007-04-04 | 株式会社中央疫苗研究所 | Inactivated mixed vaccine for porcine respiratory disease and the method of manufacturing thereof |
| WO2008085406A3 (en) * | 2006-12-28 | 2008-09-18 | Newport Lab Inc | Modified live (jmso strain) haemophilus parasuis vaccine |
-
2010
- 2010-12-15 CN CN201010596059.3A patent/CN102559531B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1942204A (en) * | 2004-04-26 | 2007-04-04 | 株式会社中央疫苗研究所 | Inactivated mixed vaccine for porcine respiratory disease and the method of manufacturing thereof |
| WO2008085406A3 (en) * | 2006-12-28 | 2008-09-18 | Newport Lab Inc | Modified live (jmso strain) haemophilus parasuis vaccine |
Non-Patent Citations (4)
| Title |
|---|
| 副猪嗜血杆菌培养条件的筛选及对豚鼠的致病性试验;高鹏程;《动物医学进展》;20081231;第29卷(第10期);全文 * |
| 副猪嗜血杆菌培养特性及致病性研究;王海燕;《金陵科技学院学报》;20091231;第25卷(第4期);全文 * |
| 王海燕.副猪嗜血杆菌培养特性及致病性研究.《金陵科技学院学报》.2009,第25卷(第4期), |
| 高鹏程.副猪嗜血杆菌培养条件的筛选及对豚鼠的致病性试验.《动物医学进展》.2008,第29卷(第10期), |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102559531A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102559531B (en) | Haemophilus parasuis 5 type high-density fermentation medium and application thereof | |
| CN102492673B (en) | Method for producing alternan sucrase by fermenting Leuconostoccitreum and its application | |
| CN110408607B (en) | Fermentation optimization process for producing hyaluronidase by lactobacillus plantarum | |
| CN103232962A (en) | High-density fermentation culture medium for haemophilus parasuis and preparation method for same | |
| CN109468355A (en) | A method of improving fermenting and producing hyaluronan molecule amount | |
| CN102964152A (en) | Culture medium formula and preparation method of white beech mushroom liquid spawn | |
| CN102268469A (en) | Preparation method of cyclic adenosine monophosphate | |
| CN106591146B (en) | A kind of fermentation culture method of fumosorosea | |
| CN103937714A (en) | Bacterial strain producing acid urease and EC degrading enzyme and application of bacterial strain | |
| CN103667160B (en) | A kind of haemophilus parasuis high density fermentation culture medium | |
| CN113046253B (en) | Culture method for improving heat resistance of kluyveromyces marxianus | |
| CN105420143B (en) | A kind of east acetobacter and its method for producing astragalus polyose | |
| CN110029076A (en) | A kind of compound caproic acid bacteria solution and preparation method thereof for producing Luzhou-flavor liquo | |
| CN102071165A (en) | Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid | |
| CN106434479B (en) | High-density culture method of haemophilus parasuis 5 type 500L fermentation tank | |
| CN106479932A (en) | A kind of Lactobacillus pentosus producing gamma aminobutyric acid | |
| CN107586744B (en) | Culture medium for culturing streptococcus pneumoniae | |
| CN102994433B (en) | Acid producing klebsiella oxytoca for synthesizing acidity ethyl carbamate hydrolase and application thereof | |
| CN112501077B (en) | Method for culturing clostridium butyricum | |
| CN109082388A (en) | E. coli isolated from ducks high density fermentation culture medium and preparation method thereof | |
| CN105175275A (en) | Separation and purification method of L-ornithine | |
| CN107217007B (en) | Fermentation medium for producing PF1022A and fermentation method | |
| CN113817632A (en) | Streptococcus thermophilus, sleep-aiding fermented milk base material rich in GABA (gamma-aminobutyric acid), lactobacillus beverage and preparation method | |
| CN102533577B (en) | Type 2 streptococcus suis high-intensity fermentation medium and application | |
| CN106434779A (en) | Application of lactobacillus pentosus SS6 to producing gamma-aminobutyric acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 430070 1 Lion Rock street, Hongshan District, Wuhan, Hubei Co-patentee after: Huazhong Agricultural University Patentee after: WUHAN KEQIAN BIOLOGICAL CO., LTD. Address before: 430070 1 Lion Rock street, Hongshan District, Wuhan, Hubei Co-patentee before: Huazhong Agricultural University Patentee before: Wuhan Keqian Animal Biological Products Co., Ltd. |