WO2008085406A3 - Modified live (jmso strain) haemophilus parasuis vaccine - Google Patents

Modified live (jmso strain) haemophilus parasuis vaccine Download PDF

Info

Publication number
WO2008085406A3
WO2008085406A3 PCT/US2007/026153 US2007026153W WO2008085406A3 WO 2008085406 A3 WO2008085406 A3 WO 2008085406A3 US 2007026153 W US2007026153 W US 2007026153W WO 2008085406 A3 WO2008085406 A3 WO 2008085406A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
strain
haemophilus parasuis
jmso
modified live
Prior art date
Application number
PCT/US2007/026153
Other languages
French (fr)
Other versions
WO2008085406A2 (en
Inventor
Simone R Oliveira
Randy R Simonson
Jonathan D Mahlberg
Mark D Titus
Tracy A Oleson
Original Assignee
Newport Lab Inc
Simone R Oliveira
Randy R Simonson
Jonathan D Mahlberg
Mark D Titus
Tracy A Oleson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Newport Lab Inc, Simone R Oliveira, Randy R Simonson, Jonathan D Mahlberg, Mark D Titus, Tracy A Oleson filed Critical Newport Lab Inc
Publication of WO2008085406A2 publication Critical patent/WO2008085406A2/en
Publication of WO2008085406A3 publication Critical patent/WO2008085406A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36—Adaptation or attenuation of cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/21—Haemophilus

Abstract

The present invention is a culture of cells of Haemophilus parasuis exhibiting attenuated pathogenicity and capable of triggering a protective immune response when administered to pigs as a live vaccine. The present invention is also a method of preparing the cell culture, a method of preparing a vaccine from the cell culture, and a live vaccine based on the cell culture. The cell culture was modified from a pathogenic parent strain by MNNG mutagenesis and was selected by complete dependence on streptomycin for growth.
PCT/US2007/026153 2006-12-28 2007-12-20 Modified live (jmso strain) haemophilus parasuis vaccine WO2008085406A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/648,390 US8404253B2 (en) 2006-12-28 2006-12-28 Modified live (JMSO strain) Haemophilus parasuis vaccine
US11/648,390 2006-12-28

Publications (2)

Publication Number Publication Date
WO2008085406A2 WO2008085406A2 (en) 2008-07-17
WO2008085406A3 true WO2008085406A3 (en) 2008-09-18

Family

ID=39493472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/026153 WO2008085406A2 (en) 2006-12-28 2007-12-20 Modified live (jmso strain) haemophilus parasuis vaccine

Country Status (2)

Country Link
US (1) US8404253B2 (en)
WO (1) WO2008085406A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102559531B (en) * 2010-12-15 2014-03-19 武汉科前动物生物制品有限责任公司 Haemophilus parasuis 5 type high-density fermentation medium and application thereof

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8404253B2 (en) * 2006-12-28 2013-03-26 Newport Laboratories, Inc. Modified live (JMSO strain) Haemophilus parasuis vaccine
US9132183B2 (en) * 2007-03-09 2015-09-15 Newport Laboratories Modified live (JMSO strain) Haemophilus parasuis vaccine
EP2280725B1 (en) * 2008-03-26 2012-05-23 Intervet International B.V. Vaccine for protection against haemophilus parasuis serotype 4 in piglets
US8911743B2 (en) * 2009-11-04 2014-12-16 Regents Of The University Of Minnesota Haemophilus parasuis polypeptides and methods of use
US20130039941A1 (en) 2010-04-23 2013-02-14 Intervet International B.V. Vaccine comprising inactivated cells of haemophilus parasuis bacteria of serotype 5
EP3052126A2 (en) * 2013-10-04 2016-08-10 Merial, Inc. Haemophilus parasuis vaccine serovar type four
JP6342011B2 (en) 2014-04-01 2018-06-13 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Prodrugs of HIV reverse transcriptase inhibitors
CN106591189A (en) * 2016-12-16 2017-04-26 青岛易邦生物工程有限公司 Haemophilus parasuis strain

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2103355C1 (en) * 1995-11-23 1998-01-27 Государственное Федеральное предприятие Государственный научный центр прикладной микробиологии Method of preparing attenuated immunogenic strains of pasteurella multocida
WO2004064776A2 (en) * 2003-01-14 2004-08-05 Mississippi State University Methods of preparation of live attenuated bacterial vaccines by alteration of dna adenine methylase (dam) activity in those bacteria

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6783764B1 (en) * 1997-04-10 2004-08-31 Akzo Nobel Nv Actinobacillus pleuropneumoniae subunit vaccine
US20020068068A1 (en) * 1999-02-02 2002-06-06 Mahan Michael J. Method of creating antibodies and compositions used for same
KR20070064666A (en) * 2004-11-11 2007-06-21 화이자 프로덕츠 인코포레이티드 Mutant porcine reproductive and respiratory syndrome virus
ES2330904B1 (en) * 2005-09-21 2010-09-23 Fundacio Centre De Recerca En Sanitat Animal HAEMOPHILUS PARASUIS POLINUCLEOTIDES AND ITS USE.
PL2007419T3 (en) * 2006-04-10 2012-09-28 Intervet Int Bv Vaccine against mycoplasma prrsv
US8404253B2 (en) * 2006-12-28 2013-03-26 Newport Laboratories, Inc. Modified live (JMSO strain) Haemophilus parasuis vaccine
US8911743B2 (en) * 2009-11-04 2014-12-16 Regents Of The University Of Minnesota Haemophilus parasuis polypeptides and methods of use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2103355C1 (en) * 1995-11-23 1998-01-27 Государственное Федеральное предприятие Государственный научный центр прикладной микробиологии Method of preparing attenuated immunogenic strains of pasteurella multocida
WO2004064776A2 (en) * 2003-01-14 2004-08-05 Mississippi State University Methods of preparation of live attenuated bacterial vaccines by alteration of dna adenine methylase (dam) activity in those bacteria
US20070082009A1 (en) * 2003-01-14 2007-04-12 Lawrence Mark L Methods of preparation of live attenuated bacterial vaccine by alteration of dna adenine methylase (dam) activity in those bacteria

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BIGAS A ET AL: "Development of a genetic manipulation system for Haemophilus parasuis", VETERINARY MICROBIOLOGY, AMSTERDAM, NL, vol. 105, no. 3-4, 25 February 2005 (2005-02-25), pages 223 - 228, XP004740821, ISSN: 0378-1135 *
BIGAS ANNA ET AL: "Colonization capacity and serum bactericidal activity of Haemophilus parasuis thy mutants", INTERNATIONAL MICROBIOLOGY, vol. 9, no. 4, December 2006 (2006-12-01), pages 297 - 301 URL, XP009101779, ISSN: 1139-6709 *
DATABASE EMBL 16 April 2005 (2005-04-16), "Haemophilus parasuis ribosomal protein S12 (rps12) gene, complete cds", XP002484740, retrieved from EBI Database accession no. AY236072 *
HOOKE A M ET AL: "Live attenuated bacterial vaccines: new approaches for safety and efficacy.", LANCET 29 JUN 1985, vol. 1, no. 8444, 29 June 1985 (1985-06-29), pages 1472 - 1474, XP002484737, ISSN: 0140-6736 *
LINDE K ET AL: "STABLE LISTERIA MONOCYTOGENES LIVE VACCINE CANDIDATE STRAINS WITH GRADED ATTENUATION ON THE MOUSE MODEL", VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 9, no. 2, 1 February 1991 (1991-02-01), pages 101 - 105, XP000175534, ISSN: 0264-410X *
LINDE K: "[Use of attenuated mutants of Pasteurella multocida strain, pathogenic to calves, in experimental mouse model studies. 4. Immunogenicity of selected revertants of temperature-sensitive, streptomycin-dependent mutants as potential vaccine strains]", ARCHIV FÜR EXPERIMENTELLE VETERINÄRMEDIZIN 1978, vol. 32, no. 6, 1978, pages 943 - 949, XP009101801, ISSN: 0003-9055 *
NIELSEN R: "Pathogenicity and immunity studies of Haemophilus parasuis serotypes", ACTA VETERINARIA SCANDINAVICA, vol. 34, no. 2, 1993, pages 193 - 198, XP009101790, ISSN: 0044-605X *
OLIVEIRA SIMONE ET AL: "Haemophilus parasuis: New trends on diagnosis, epidemiology and control.", VETERINARY MICROBIOLOGY, vol. 99, no. 1, 26 March 2004 (2004-03-26), pages 1 - 12, XP002484736, ISSN: 0378-1135 *
STUY J H ET AL: "Cloning, characterization, and DNA base sequence of the high-level streptomycin resistance gene strA1 of Haemophilus influenzae Rd.", JOURNAL OF BACTERIOLOGY SEP 1992, vol. 174, no. 17, September 1992 (1992-09-01), pages 5604 - 5608, XP002484739, ISSN: 0021-9193 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102559531B (en) * 2010-12-15 2014-03-19 武汉科前动物生物制品有限责任公司 Haemophilus parasuis 5 type high-density fermentation medium and application thereof

Also Published As

Publication number Publication date
WO2008085406A2 (en) 2008-07-17
US8404253B2 (en) 2013-03-26
US20100255035A1 (en) 2010-10-07

Similar Documents

Publication Publication Date Title
WO2008085406A3 (en) Modified live (jmso strain) haemophilus parasuis vaccine
Yuan et al. Identity, diversity, and molecular phylogeny of the endophytic mycobiota in the roots of rare wild rice (Oryza granulate) from a nature reserve in Yunnan, China
NZ595914A (en) Vaccines comprising attenuated mycoplasma bovis strains and method for the attenuation
WO2010019262A3 (en) Polyvalent vaccine
MX2011007551A (en) Saccharomyces cerevisiae strains with phytosanitary capabilities.
DK2473189T3 (en) Methods for reducing virucid activity in PCV-2 compositions and PCV-2 compositions with improved immunogenicity.
WO2010132112A3 (en) Immunogenic compositions against tuberculosis
WO2013000717A3 (en) Dermatologically effective yeast extract
NZ586238A (en) Methods and compositions for immunizing pigs against porcine circovirus
WO2007024941A3 (en) Polyvalent vaccine
WO2006127150A3 (en) Dendritic cell compositions and methods
IN2014DN08830A (en)
MY144689A (en) Dengue serotype 1 attenuated strain
WO2007089677A3 (en) Systems and methods for producing biofuels and related materials
NZ585777A (en) Compositions and methods of enhancing immune responses to eimeria
WO2005089447A3 (en) Streptococcus agalactiae vaccine
WO2009103960A3 (en) Methods and compositions for modifying plant flavonoid composition and disease resistance
NZ603918A (en) Improved methods for preparing squalene
WO2008012538A3 (en) Live vaccine strains of francisella
CA2849393C (en) Phytoseiid predatory mite releasing system and method for production.
UA98506C2 (en) Attenuated mycoplasma gallisepticum strains
NZ589981A (en) Cattle vaccines comprising a disrupted M. bovis bacterium
NO20060976L (en) Vaccine
WO2011159092A3 (en) Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
MX2016003676A (en) Isolated bacterium of the genus streptomyces.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07863197

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07863197

Country of ref document: EP

Kind code of ref document: A2