CN102552777A - Medicament for treating herpes simplex keratitis and preparation method thereof - Google Patents
Medicament for treating herpes simplex keratitis and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a medicament for treating herpes simplex keratitis, which is characterized in that the medicament is mainly prepared by the following raw material herbs by weight: 10-15 parts of radix sophorae flavescentis, 10-15 parts of radix stemonae, 15-20 parts of equisetum, 10-15 parts of isatis roots, 7-10 parts of angelica, 10-15 parts of divaricate saposhnikovia roots, 7-10 parts of common carpesium fruits, 10-15 parts of butterflybush flowers, 5-7 parts of lithospermum, and 15-20 parts of wild chrysanthemum flowers. The medicament for treating herpes simplex keratitis prepared by the invention has less side effect, a low recurrence rate, strong antiviral effect, and significant treatment effect. The invention also provides a preparation method of the medicament.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition with and preparation method thereof, particularly, the present invention relates to a kind of medicine of treating herpes simplex keratitis with and preparation method thereof.
Background technology
Herpes simplex keratitis (Herpes simplex keratitis; HSK) be the modal infectious keratopathy of clinical ophthalmology; Be that (Herpes simplex virus tye 1 is HSV-I) due to the infection, with its high relapse rate for the I herpes simplex virus type; High blind rate serious harm patient's vision, the live and work that influence is human.Tool China city is the survey result report blindly, and corneal blindness accounts for second, and the HSK blinding accounts for corneal blindness first place (42.8%).HSK can cause latent infection, makes its course of disease protracted course of disease, the lighter's visual deterioration, and weight person is blind, and consequence is serious.
To this sick medicine, be mostly eye drop class Western medicine prepn at present, side effect is big, relapse rate is high, antiviral effect is undesirable, is prone to produce drug resistance.
Therefore, a kind of few side effects is provided, the Chinese medicine medicine of the treatment herpes simplex keratitis that antivirus action is strong is that this case inventor is desired always.
Summary of the invention
The Chinese medicine medicine that the object of the present invention is to provide a kind of few side effects, relapse rate is low, antivirus action is strong, therapeutic effect is treated herpes simplex keratitis significantly.The present invention also provides the method for preparing of this medicine.
For this reason, the invention provides a kind of medicine of treating herpes simplex keratitis, it is characterized in that it mainly is to be processed by following bulk drugs: Radix Sophorae Flavescentis 10-15 part; Radix Stemonae 10-15 part, Herba Equiseti Hiemalis 15-20 part, Radix Isatidis 10-15 part; Radix Angelicae Sinensis 7-10 part, windproof 10-15 part, Fructus Carpesii 7-10 part; Flos Buddlejae 10-15 part, Radix Arnebiae (Radix Lithospermi) 5-7 part, Flos Chrysanthemi Indici 15-20 part.The medicine of the above-mentioned treatment herpes simplex keratitis of the present invention; It is characterized in that: described medicine also comprises following bulk drugs: Fructus Leonuri 7-10 part; Fructus Atriplicis Sibiricae 7-10 part further can also comprise following bulk drugs: Periostracum Cicadae 7-10 part, Radix Astragali 7-10 part.
The present invention also provides a kind of manufacturing method for above mentioned medicine, it is characterized in that this method comprises the following steps:
A. take by weighing said bulk drugs;
B. the above-mentioned raw materials medicine that is taken by weighing is placed and fry in shallow oil in the device, decocte with water is crossed and is filtered crude drug filtering residue and medicinal liquid, leaves and takes medicinal liquid; Decocte with water in the crude drug filtering residue again, filtering extracting liquid with twice combining medicine, promptly gets described medicine once more.
Further, method for preparing of the present invention comprises the following steps:
A. take by weighing said bulk drugs;
B. the above-mentioned raw materials medicine that is taken by weighing is placed and fry in shallow oil in the device, add water, the weight of water is 5-9 times of crude drug gross weight, decocts 20-50 minute, crosses and filters crude drug filtering residue and medicinal liquid, leaves and takes medicinal liquid; In the crude drug filtering residue, add water again, the weight of water is 3-6 times of crude drug gross weight, decocts 10-30 minute, and filtering extracting liquid with twice combining medicine, concentrates once more, promptly gets described medicine.
Medicine of the present invention is characterized in that described pharmaceutical dosage form is an oral agents, and wherein said oral agents is decoction or unguentum.
It is known to those skilled in the art that the adjuvant and the auxiliary agent that can comprise any routine in this medicine, this does not exceed scope of the present invention.
Medicine of the present invention can be treated herpes simplex keratitis effectively, and the pure Chinese medicinal preparation few side effects, relapse rate is low, antivirus action is strong, therapeutic effect is remarkable.
In order to understand the present invention better, combine specific embodiment that the present invention is at length explained now.Said embodiment only is to example of the present invention, does not mean any restriction of the present invention.
The specific embodiment
Embodiment 1: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 5L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 2: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 5L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 3: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 12.5L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 8.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 4: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 12.5L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 8.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 5: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 8.5L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 5.5L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 6: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 8.5L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 5.5L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 7: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g, Fructus Leonuri 70g, Fructus Atriplicis Sibiricae 70g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 6L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 8: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g, Fructus Leonuri 70g, Fructus Atriplicis Sibiricae 70g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 6L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 9: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g, Fructus Leonuri 100g, Fructus Atriplicis Sibiricae 100g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 14.5L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 9.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 10: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g, Fructus Leonuri 100g, Fructus Atriplicis Sibiricae 100g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 14.5L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 9.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 11: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g, Fructus Leonuri 85g, Fructus Atriplicis Sibiricae 85g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 9.5L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 6L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 12: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g, Fructus Leonuri 85g, Fructus Atriplicis Sibiricae 85g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 9.5L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 6L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 13: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g, Fructus Leonuri 70g, Fructus Atriplicis Sibiricae 70g, Periostracum Cicadae 70g, Radix Astragali 70g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 6.5L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 14: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 100g, Radix Stemonae 100g, Herba Equiseti Hiemalis 150g, Radix Isatidis 100g, Radix Angelicae Sinensis 70g, windproof 100g, Fructus Carpesii 70g, Flos Buddlejae 100g, Radix Arnebiae (Radix Lithospermi) 50g, Flos Chrysanthemi Indici 150g, Fructus Leonuri 70g, Fructus Atriplicis Sibiricae 70g, Periostracum Cicadae 70g, Radix Astragali 70g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 6.5L, decocted 20 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 4L again, decocted 10 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 15: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g, Fructus Leonuri 100g, Fructus Atriplicis Sibiricae 100g, Periostracum Cicadae 100g, Radix Astragali 100g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 16L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 10.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 16: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 150g, Radix Stemonae 150g, Herba Equiseti Hiemalis 200g, Radix Isatidis 150g, Radix Angelicae Sinensis 100g, windproof 150g, Fructus Carpesii 100g, Flos Buddlejae 150g, Radix Arnebiae (Radix Lithospermi) 70g, Flos Chrysanthemi Indici 200g, Fructus Leonuri 100g, Fructus Atriplicis Sibiricae 100g, Periostracum Cicadae 100g, Radix Astragali 100g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 16L, decocted 50 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 10.5L again, decocted 30 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Embodiment 17: the preparation of the Chinese medicine decoction of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g, Fructus Leonuri 85g, Fructus Atriplicis Sibiricae 85g, Periostracum Cicadae 85g, Radix Astragali 85g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 10L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 7L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, promptly gets described Chinese medicine decoction once more.
Embodiment 18: the preparation of the Chinese medicine unguentum of treatment herpes simplex keratitis
Take by weighing the crude drug of following weight: Radix Sophorae Flavescentis 120g, Radix Stemonae 120g, Herba Equiseti Hiemalis 170g, Radix Isatidis 120g, Radix Angelicae Sinensis 80g, windproof 130g, Fructus Carpesii 90g, Flos Buddlejae 120g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Chrysanthemi Indici 180g, Fructus Leonuri 85g, Fructus Atriplicis Sibiricae 85g, Periostracum Cicadae 85g, Radix Astragali 85g.
The above-mentioned raw materials medicine that is taken by weighing placed fry in shallow oil in the device, add water 10L, decocted 30 minutes, cross and filter crude drug filtering residue and medicinal liquid, leave and take medicinal liquid; In the crude drug filtering residue, add water 7L again, decocted 20 minutes, filtering extracting liquid with twice combining medicine, concentrates with 1: 10 ratio once more, promptly gets described Chinese medicine unguentum.
Experimental example 1: clinical statistics is learned data
To making a definite diagnosis different course of disease patient's oral administration embodiment of the invention 1 prepared decoctions of suffering from herpes simplex keratitis, every day 2 times, each 250ml, 2 weeks of the course of treatment.In 1 week of drug withdrawal, then, every day 2 times, each 250ml in 2 weeks of the course of treatment, finishes the course of treatment.
Recurrence situation statistics after the therapeutic effect of table 1: embodiment 1 decoction and the treatment recovery from illness
Can find out that from the data of table 1 decoction of the present invention all has good curative effect to the patient of the different courses of disease, relapse rate is extremely low simultaneously.
Carried out above-mentioned clinical statistics with prepared decoction of embodiment 2-18 and unguentum, the result is similar.
Experimental example 2: the antiviral experimentation of the external pastille serum of Drug therapy herpes simplex keratitis
In order to assess the clinical effectiveness of the obtained Drug therapy of the present invention, this research is to viral keratitis experimental animal models random packet, take the Chinese medicine of various dose after, the antiviral experiment of the external pastille serum of monitoring different time.
1. materials and methods
(1) experiment material
1. receive reagent thing: embodiment 6 prepared unguentum.The rabbit dosage is respectively 33g/kg (constant), 165g/kg (doubly amount); Rabbit administration every day 2 times in 2 weeks of successive administration, is put to death rabbit, separation of serum.The serum dilution is 1 during the experiment of pastille rabbit anteserum: 4-1: 32 4 dilution factors are used for experiment.
2. positive control drug: Western medicine acyclovir eye drop.The rabbit consumption is 3 times/day, drips/inferior.
3. virus: herpesvirus I, II type be available from Inst. of Viruses, China Preventive Medicine Science Academy, and Chinese medicine institute of Chinese department of Chinese medicine institute Viral Laboratory uses at the back of going down to posterity.
4. cell: people's laryngeal carcinoma passage cell HEP-2 strain, available from Ministry of Public Health pharmaceutical biological product calibrating institute.After going down to posterity, China Chinese medicine institute of department of Chinese medicine institute Viral Laboratory uses.
5. cell culture fluid: contain that 10% calf serum, 0.29mg glutamine/ml, 100u/ml are blue or green, the Eagle ' sMEM (a Japan day water Pharmaceutical Co., Ltd produces) of streptomycin.
6. cell maintenance medium removes contained calf serum and is 2%, 0.29mg glutamine/ml, 100u/ml are blue or green, the Eagle ' sMEM (a Japan day water Pharmaceutical Co., Ltd produces) of streptomycin.
(2) key instrument and equipment
1. CO
2Constant incubator (95%O
2-5%CO
2Gaseous mixture) (the loose Co., Ltd. of Japanese Yamato product)
2. inverted phase contrast microscope (Japanese Olympus company)
3. superclean bench (Beijing semiconductor equipment factory), operating theater instruments, magnetic stirring apparatus, stirrer
4. electrically heated drying cabinet, moist heat sterilization jar, constant water bath box (the dark China of China) ,-20 ℃ of refrigerators (Chinese Haier)
5. microporous filter membrane filter
2 experimental techniques and step
(1) the pastille rabbit anteserum is to the toxicity test of Hep-2 cultured cell
The pastille rabbit anteserum is done 1 with Eagle ' s culture fluid: 2-1: behind 32 doubling dilutions, be added in the Hep-2 Tissue Culture Plate that grows up to monolayer, the 100ul/ hole, each dilution factor medicinal liquid is done 4 multiple holes, establishes the normal cell contrast simultaneously.Culture plate is put 37 ℃ of 5%CO
2Cultivated in the incubator four days, observation of cell growing state under the every day inverted microscope confirms that the minimum extension rate (Cmax) of obvious regression does not appear in cell, and four concentration postpone during experiment.
Table 2: pastille serum is to the TC of cultured cell
0Mensuration
(2) to the influence of pathological changes caused by virus effect
Get the culture plate that grows up to cell monolayer, outwell culture fluid, inoculation 100TCID
50Viral liquid 50ul, put 37 ℃ of 5%CO
2Absorption was outwelled viral liquid after 1 hour in the incubator, after keeping liquid and wash cell face 3 times with the Eagle ' s that does not contain calf serum, added corresponding dilution pastille serum 100ul/ hole.Establish model contrast, positive drug contrast medicine and normal rabbit serum contrast simultaneously.Put 37 ℃ of 5%CO
2Cultivate in the incubator, observation of cell pathological changes under the every day inverted microscope, when the model control group cytopathy does ++ ++ the time record experimental result.Cytopathy is judged by six grade standards, is carried out statistical procedures with rank test.
-: the cell growth is normal, and no pathological changes occurs;
±: cytopathy is less than 10% of whole monolayer;
1: cytopathy accounts for below 25% of whole cell monolayer;
2: cytopathy accounts for below 50% of whole cell monolayer;
3: cytopathy accounts for below 75% of whole cell monolayer;
4: cytopathy accounts for more than 75% of whole cell monolayer.
3. result
Can find out that from the result of table 3-7 1: 4,1: 8 dilution factor of pastille serum that pastille serum 1: 4,1: 8 dilution factor, the constant of two dose groups of unguentum of the present invention add Western medicine group, western medicine group all has the obvious suppression effect to HSV-1; And two dose groups drug effects of unguentum of the present invention are similar with western medicine group, and Western medicine adds Chinese medicine constant group also has the good restraining effect to HSV-1.
Table 3: the external anti-HSV-1 experimental result of unguentum pastille serum of the present invention (taking medicine 3 days)
Table 4: the external anti-HSV-1 experimental result of unguentum pastille serum of the present invention (taking medicine 10 days)
Table 5: the external anti-HSV-1 experimental result of unguentum pastille serum of the present invention (taking medicine 17 days)
Table 6: the external anti-HSV-1 experimental result of unguentum pastille serum of the present invention (taking medicine 24 days)
Table 7: the external anti-HSV-1 experimental result of unguentum pastille serum of the present invention (taking medicine 34 days)
Simultaneously, used embodiment 1-5 to carry out above-mentioned identical experiment with the prepared medicine of embodiment 7-18, the result is similar.
Claims (7)
1. a medicine of treating herpes simplex keratitis is characterized in that it mainly is to be processed by following bulk drugs: Radix Sophorae Flavescentis 10-15 part, Radix Stemonae 10-15 part; Herba Equiseti Hiemalis 15-20 part, Radix Isatidis 10-15 part, Radix Angelicae Sinensis 7-10 part; Windproof 10-15 part, Fructus Carpesii 7-10 part, Flos Buddlejae 10-15 part; Radix Arnebiae (Radix Lithospermi) 5-7 part, Flos Chrysanthemi Indici 15-20 part.
2. the medicine of treatment herpes simplex keratitis according to claim 1 is characterized in that it also comprises following bulk drugs: Fructus Leonuri 7-10 part, Fructus Atriplicis Sibiricae 7-10 part.
3. the medicine of treatment herpes simplex keratitis according to claim 2 is characterized in that it also comprises following bulk drugs: Periostracum Cicadae 7-10 part, Radix Astragali 7-10 part.
4. the method for preparing of the said medicine of arbitrary claim in the claim 1 to 3 is characterized in that this method comprises the following steps:
A. take by weighing said bulk drugs;
B. the above-mentioned raw materials medicine that is taken by weighing is placed and fry in shallow oil in the device, decocte with water is crossed and is filtered crude drug filtering residue and medicinal liquid, leaves and takes medicinal liquid; Decocte with water in the crude drug filtering residue again, filtering extracting liquid with twice combining medicine, promptly gets described medicine once more.
5. according to the method for preparing of the said medicine of claim 4, it is characterized in that this method comprises the following steps:
A. take by weighing said bulk drugs;
B. the above-mentioned raw materials medicine that is taken by weighing is placed and fry in shallow oil in the device, add water, the weight of water is 5-9 times of crude drug gross weight, decocts 20-50 minute, crosses and filters crude drug filtering residue and medicinal liquid, leaves and takes medicinal liquid; In the crude drug filtering residue, add water again, the weight of water is 3-6 times of crude drug gross weight, decocts 10-30 minute, and filtering extracting liquid with twice combining medicine, concentrates once more, promptly gets described medicine.
6. according to the described medicine of arbitrary claim in the claim 1 to 3, it is characterized in that described pharmaceutical dosage form is an oral agents.
7. medicine according to claim 6 is characterized in that described oral agents is decoction or unguentum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210033510XA CN102552777A (en) | 2012-02-15 | 2012-02-15 | Medicament for treating herpes simplex keratitis and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102961521A (en) * | 2012-12-11 | 2013-03-13 | 王正艳 | Drug for treating herpes simplex virus keratities |
| CN103041066A (en) * | 2012-12-29 | 2013-04-17 | 青岛恒波仪器有限公司 | Traditional Chinese medicine composition for curing herpes simplex viral keratitis and preparing method thereof |
| CN105770248A (en) * | 2016-04-21 | 2016-07-20 | 毕宏生 | Traditional Chinese medicine composition for treating keratitis as well as preparation method and application thereof |
| CN105816601A (en) * | 2016-04-21 | 2016-08-03 | 毕宏生 | Medicine composition for treating viral keratitis |
| CN106177661A (en) * | 2016-08-15 | 2016-12-07 | 华梅 | A kind of Chinese medicine composition treating herpes simplex keratitis and preparation method thereof |
| CN109700937A (en) * | 2019-03-04 | 2019-05-03 | 中国中医科学院眼科医院 | A kind of Chinese prescription for treating herpes simplex keratitis and reduce its recurrence |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102961521A (en) * | 2012-12-11 | 2013-03-13 | 王正艳 | Drug for treating herpes simplex virus keratities |
| CN102961521B (en) * | 2012-12-11 | 2014-06-11 | 王正艳 | Drug for treating herpes simplex virus keratities |
| CN103041066A (en) * | 2012-12-29 | 2013-04-17 | 青岛恒波仪器有限公司 | Traditional Chinese medicine composition for curing herpes simplex viral keratitis and preparing method thereof |
| CN105770248A (en) * | 2016-04-21 | 2016-07-20 | 毕宏生 | Traditional Chinese medicine composition for treating keratitis as well as preparation method and application thereof |
| CN105816601A (en) * | 2016-04-21 | 2016-08-03 | 毕宏生 | Medicine composition for treating viral keratitis |
| CN106177661A (en) * | 2016-08-15 | 2016-12-07 | 华梅 | A kind of Chinese medicine composition treating herpes simplex keratitis and preparation method thereof |
| CN109700937A (en) * | 2019-03-04 | 2019-05-03 | 中国中医科学院眼科医院 | A kind of Chinese prescription for treating herpes simplex keratitis and reduce its recurrence |
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