CN102533538B - Aeration control system for photobioreactor - Google Patents
Aeration control system for photobioreactor Download PDFInfo
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- CN102533538B CN102533538B CN201010600721.8A CN201010600721A CN102533538B CN 102533538 B CN102533538 B CN 102533538B CN 201010600721 A CN201010600721 A CN 201010600721A CN 102533538 B CN102533538 B CN 102533538B
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- bioreactor
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- supply flow
- air feeder
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The invention discloses an aeration control system for a photobioreactor. The aeration control system comprises an illuminance sensor, a turbidity sensor, an air supply device, and a controller; the illuminance sensor is used for measuring light intensity around the photobioreactor; the turbidity sensor is used for measuring the concentration of light organisms in the photobioreactor; and the air supply device is used for supplying aeration air to the photobioreactor; and the controller adjusts the supplied air flow of the air supply device according to the light intensity which is measured by the illuminance sensor and the concentration of the light organisms, which is measured by the turbidity sensor. Therefore, the aeration quantity can be adjusted according to the light intensity and the concentration of algae liquid. When the light is intense, the aeration quantity is large, and stirring velocity is increased; when the light is weak, the aeration quantity is small, and the stirring velocity is reduced; meanwhile, when the concentration of the algae liquid is high, the aeration quantity is increased; when the concentration is low, the aeration quantity is reduced, so that the optimum aeration stirring intensity for growth of microalgae is kept. Therefore, the yield of the microalgae is increased, energy consumption of the aeration is reduced, and production cost is reduced.
Description
Technical field
The present invention relates to a kind of aeration control system for bioreactor.
Background technology
Micro-algae effectively can utilize luminous energy, carbonic acid gas, water and minerals synthetic protein, fat, carbohydrate and high added value biologically active substance, algae is because having high light and nutritional utilization efficiency shows the growth potential stronger than higher plant, and therefore the cultivation of micro-algae is in widespread attention.
At present, the open pool of the many employings of large-scale industrial production of micro-algae, it is unstable, easily contaminated to there is production in this production model, and micro-algae light is uneven, the problems such as the efficiency of light energy utilization is not high, and culture efficiency is low.Thus the development of the production of micro-algae and microalgae biotechnology industry is constrained.Recent domestic scholar has carried out a series of research for this respect problem, especially utilizes the research of the micro-algae of photoreactor high-efficient culture to achieve certain achievement.Bioreactor representative at present mainly contains following several: pipeline reactor, plate-type reactor, column reactor etc.These reactors illustrate good development prospect in optimal control, raising output, but also there are some problems in them in practical application, as the efficiency of light energy utilization is low, gaseous interchange is not smooth, especially at present bioreactor manufacturing cost is high, and cultivation stage energy consumption is high, the high subject matter having become the development of restriction photoreactor of cost.
At present, in plate-type reactor and column reactor system, the method of usual employing aeration agitation, the small bubbles of aerating apparatus by generation and the density difference of surrounding algae liquid, bubble floating and algae liquid is impelled to decline, produce numerous gas-liquid thus in ring-type on-swimmer's pool to circulate up and down, make the even light of micro-algae, improve light utilization efficiency, and the floating of bubble enhances the desorb of dissolved oxygen in algae liquid, oxygen is brought to liquid level by bubble and discharges, and carbon dioxide is also dissolved in algae liquid after aeration simultaneously, for the growth of micro-algae provides carbon source.But this mode needs a large amount of pressurized air, energy consumption is very high, is unfavorable for the scale operation of micro-algae.Meanwhile, the too high and too low all unfavorable growth having micro-algae of the intensity of aeration agitation.
Summary of the invention
The invention provides a kind of aeration control system for bioreactor, it can according to light intensity and algae liquid concentration, adjustment aeration rate.During light intensity, aeration rate is large, and strengthen stir speed (S.S.), when light is weak, aeration rate is little, reduces stir speed (S.S.); Equally, when algae liquid concentration height, tune up aeration rate, when concentration is low, turn aeration rate down, thus make aeration agitation intensity be in the best stirring intensity of micro algae growth always.Therefore, the present invention reduces aeration energy consumption while the micro-algae output of raising, reduces production cost.
According to an aspect of the present invention, a kind of aeration control system for bioreactor is provided, comprises: illuminance sensor, for measuring the light intensity of bioreactor surrounding environment; Turbidity transducer, for measuring the concentration of the photo bio in bioreactor; Air feeder, described air feeder is used for supplying aerating gas in bioreactor; Controller, the light intensity of described controller measured by illuminance sensor and or the concentration of photo bio measured by turbidity transducer regulate the gas supply flow of described air feeder.
In a preferred embodiment of the invention, described aeration control system also comprises: flow sensor, described flow sensor is for detecting the gas supply flow of described air feeder, wherein, described controller carries out closed-loop control according to the flow feedback signal of flow sensor to the gas supply flow of described air feeder.
In another preferred embodiment of the invention, described controller is micro-chip or computer.
In another preferred embodiment of the invention, described air feeder is the blower fan with frequency transformer, changes rotation speed of fan by the frequency changing frequency transformer, thus regulates gas supply flow.
In another preferred embodiment of the invention, the light intensity measured when illuminance sensor is greater than 1200 μm of ol/m
2during/s, described controller controls air feeder with predetermined maximum gas supply flow air feed continuously.
In another preferred embodiment of the invention, the light intensity measured when illuminance sensor is greater than 800 μm of ol/m
2during/s, and when the concentration OD value of the photo bio that turbidity transducer measures is greater than 8, described controller controls air feeder with predetermined maximum gas supply flow air feed continuously.
In another preferred embodiment of the invention, the light intensity measured when illuminance sensor is at 20 μm of ol/m
2/ s to 1200 μm of ol/m
2during the scope of/s, and when the concentration OD value of the photo bio that turbidity transducer measures is less than 8, described controller controls air feeder with predetermined corresponding gas supply flow air feed continuously.
In another preferred embodiment of the invention, the light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, described controller controls air feeder with predetermined minimum gas supply flow air feed off and on.
In another preferred embodiment of the invention, length × 15 liter/min of the bioreactor of described predetermined maximum gas supply flow=in units of rice.
In another preferred embodiment of the invention, described predetermined middle gas supply flow is in the scope to length × 15 liter/min of the bioreactor in units of rice of length × 5 of the bioreactor in units of rice liter/min.
In another preferred embodiment of the invention, described predetermined minimum gas supply flow is less than length × 5 liter/min of the bioreactor in units of rice.
In another preferred embodiment of the invention, the light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, described controller controls air feeder with every 30 minutes air feed intermittent mode air feed of 2 minutes.
The present invention can detect intensity of illumination and algae liquid concentration in real time, and adjustment aerate flux, makes aeration agitation intensity be in the best stirring intensity of micro algae growth always, and therefore, the present invention reduces aeration energy consumption while the micro-algae output of raising, reduces production cost.
Accompanying drawing explanation
The functional block diagram of Fig. 1 display aeration control system according to a preferred embodiment of the present invention.
Embodiment
Below by embodiment, and by reference to the accompanying drawings, technical scheme of the present invention is described in further detail.In the description, same or analogous drawing reference numeral indicates same or analogous parts.The explanation of following reference accompanying drawing to embodiment of the present invention is intended to make an explanation to present general inventive concept of the present invention, and not should be understood to one restriction of the present invention.
The functional block diagram of Fig. 1 display aeration control system according to a preferred embodiment of the present invention.Aeration control system of the present invention is for regulating the aerate flux of the aerating apparatus in bioreactor.
As shown in Figure 1, in the first preferred embodiment of the present invention, this aeration control system mainly comprises: illuminance sensor, turbidity transducer, air feeder and controller.
As shown in Figure 1, in a preferred embodiment of the invention, illuminance sensor, on bioreactor side, for measuring the light intensity of bioreactor surrounding environment, and sends the light intensity signal measured to controller.
As shown in Figure 1, in a preferred embodiment of the invention, turbidity transducer is arranged in bioreactor, for measuring the concentration of the photo bio (such as micro-algae) in bioreactor, and sends the photo bio concentration signal measured to controller.
In one preferred embodiment, which is shown in fig. 1, the aerating apparatus gas communication in air feeder and bioreactor, for supplying aerating gas in bioreactor.
In one preferred embodiment, which is shown in fig. 1, the concentration of the light intensity of controller measured by illuminance sensor and the photo bio measured by turbidity transducer regulates the gas supply flow of air feeder.
In a preferred embodiment of the invention, the light intensity measured when illuminance sensor is greater than 1200 μm of ol/m
2/ s, controller control air feeder with predetermined maximum gas supply flow air feed continuously.In a preferred embodiment of the invention, length × 15 liter/min of the bioreactor of predetermined maximum gas supply flow=in units of rice.Such as, when the length of bioreactor is 10 meters, so predetermined maximum gas supply flow just equals 150 liters/min.
In a preferred embodiment of the invention, the light intensity measured when illuminance sensor is being greater than 800 μm of ol/m
2when the concentration OD value of the photo bio that/s and turbidity transducer measure is greater than 8, controller controls air feeder with predetermined maximum gas supply flow air feed continuously.In a preferred embodiment of the invention, length × 15 liter/min of the bioreactor of predetermined maximum gas supply flow=in units of rice.Such as, when the length of bioreactor is 10 meters, so predetermined maximum gas supply flow just equals 150 liters/min.
In a preferred embodiment of the invention, the light intensity measured when illuminance sensor is at 20 μm of ol/m
2/ s to 1200 μm of ol/m
2when the concentration OD value of the photo bio that the scope of/s and turbidity transducer measure is not more than 8, controller controls air feeder with predetermined middle gas supply flow air feed continuously.In a preferred embodiment of the invention, predetermined middle gas supply flow is in the scope to length × 15 liter/min of the bioreactor in units of rice of length × 5 of the bioreactor in units of rice liter/min.Such as, when the length of bioreactor is 10 meters, so predetermined middle gas supply flow is in the scope of 50 liters/min to 150 liters/min.
In a preferred embodiment of the invention, the light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, controller controls air feeder with every 30 minutes air feed intermittent mode air feed of 2 minutes.
As shown in Figure 1, in a preferred embodiment of the invention, aeration control system also comprises flow sensor.This flow sensor is arranged on the export pipeline of air feeder, for detecting the gas supply flow of described air feeder, and sends the gas supply flow signal detected to controller.Like this, controller just can carry out accurate closed-loop control according to the flow feedback signal of flow sensor to the gas supply flow of air feeder, thus is conducive to the precision improving flow control.But it should be noted that, not necessarily, aeration control system of the present invention also can adopt open-loop control structures to flow sensor.
In a preferred embodiment of the invention, controller can be the electronic installation that micro-chip, Personal Computer, industrial computer, flush bonding processor etc. have data processing and analysis ability.
In a preferred embodiment of the invention, air feeder for having the blower fan of frequency transformer, can change rotation speed of fan by the frequency changing frequency transformer, thus regulates gas supply flow.
In another preferred embodiment of the invention, air feeder for having electric ratio adjusting valve, by controlling electric ratio adjusting valve, can regulate gas supply flow.
It should be noted that, although the aeration control system shown in Fig. 1 comprises illuminance sensor and turbidity transducer simultaneously, the present invention is not limited to this, and aeration control system also only can comprise the one in illuminance sensor and turbidity transducer.
Although describe the present invention by reference to the accompanying drawings, embodiment disclosed in accompanying drawing is intended to carry out exemplary illustration to the preferred embodiment for the present invention, and can not be interpreted as one restriction of the present invention.
Although some embodiments of this present general inventive concept have been shown and explanation, those skilled in the art will appreciate that, when not deviating from principle and the spirit of this present general inventive concept, can make a change these embodiments, scope of the present invention is with claim and their equivalents.
Claims (7)
1., for an aeration control system for bioreactor, comprising:
Illuminance sensor, for measuring the light intensity of bioreactor surrounding environment;
Air feeder, described air feeder is used for supplying aerating gas in bioreactor; With
Controller, the light intensity of described controller measured by illuminance sensor regulates the gas supply flow of described air feeder; And the light intensity measured when illuminance sensor is greater than 1200 μm of ol/m
2during/s, described controller controls air feeder with predetermined maximum gas supply flow air feed continuously, and the light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, described controller controls air feeder with predetermined minimum gas supply flow air feed off and on; Length × 15 liter/min of the bioreactor of described predetermined maximum gas supply flow=in units of rice, described predetermined minimum gas supply flow is less than length × 5 liter/min of the bioreactor in units of rice.
2., for an aeration control system for bioreactor, comprising:
Turbidity transducer, for measuring the concentration of the photo bio in bioreactor;
Air feeder, described air feeder is used for supplying aerating gas in bioreactor; With
Controller, the concentration of the photo bio of described controller measured by turbidity transducer regulates the gas supply flow of described air feeder; And when the concentration OD value of the photo bio that turbidity transducer measures is greater than 8, described controller controls air feeder with predetermined maximum gas supply flow air feed continuously; Length × 15 liter/min of the bioreactor of described predetermined maximum gas supply flow=in units of rice.
3., for an aeration control system for bioreactor, comprising:
Illuminance sensor, for measuring the light intensity of bioreactor surrounding environment;
Turbidity transducer, for measuring the concentration of the photo bio in bioreactor;
Air feeder, described air feeder is used for supplying aerating gas in bioreactor; With
Controller, the concentration of the light intensity of described controller measured by illuminance sensor and the photo bio measured by turbidity transducer regulates the gas supply flow of described air feeder; And the light intensity measured when illuminance sensor is greater than 800 μm of ol/m
2when the concentration OD value of the photo bio that/s and turbidity transducer measure is greater than 8, described controller controls air feeder with predetermined maximum gas supply flow air feed continuously, and the light intensity measured when illuminance sensor is at 20 μm of ol/m
2/ s to 1200 μm of ol/m
2when the concentration OD value of the photo bio that the scope of/s and turbidity transducer measure is not more than 8, described controller controls air feeder with the interval gas supply flow of predetermined correspondence air feed continuously, and the light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, described controller controls air feeder with predetermined minimum gas supply flow air feed off and on; Length × 15 liter/min of the bioreactor of described predetermined maximum gas supply flow=in units of rice, described predetermined minimum gas supply flow is less than length × 5 liter/min of the bioreactor in units of rice, and the interval gas supply flow of described predetermined correspondence is in the scope to length × 15 liter/min of the bioreactor in units of rice of length × 5 of the bioreactor in units of rice liter/min.
4. the aeration control system according to claim 1,2 or 3, also comprises:
Flow sensor, described flow sensor for detecting the gas supply flow of described air feeder,
Wherein, described controller carries out closed-loop control according to the flow feedback signal of flow sensor to the gas supply flow of described air feeder.
5. aeration control system according to claim 4, is characterized in that: described controller is micro-chip or computer.
6. aeration control system according to claim 4, is characterized in that: described air feeder is the blower fan with frequency transformer, changes rotation speed of fan by the frequency changing frequency transformer, thus regulates gas supply flow.
7. aeration control system according to claim 1, is characterized in that:
The light intensity measured when illuminance sensor is less than 20 μm of ol/m
2during/s, described controller controls air feeder with every 30 minutes air feed intermittent mode air feed of 2 minutes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010600721.8A CN102533538B (en) | 2010-12-20 | 2010-12-20 | Aeration control system for photobioreactor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010600721.8A CN102533538B (en) | 2010-12-20 | 2010-12-20 | Aeration control system for photobioreactor |
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| CN102533538A CN102533538A (en) | 2012-07-04 |
| CN102533538B true CN102533538B (en) | 2015-01-14 |
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| FR3049616B1 (en) * | 2016-04-04 | 2020-09-25 | Inria Inst Nat Rech Informatique & Automatique | SELECTIVE BIOREACTOR FOR MICROALGAE |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735948A (en) * | 2010-01-11 | 2010-06-16 | 新奥科技发展有限公司 | Method and device for cultivating microalgae |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101735948A (en) * | 2010-01-11 | 2010-06-16 | 新奥科技发展有限公司 | Method and device for cultivating microalgae |
Non-Patent Citations (2)
| Title |
|---|
| 平板式光生物反应器在饵料微藻培养中的应用;孙利芹等;《烟台大学学报》;20070430;第20卷(第2期);114页左栏最后一段；图4和7 * |
| 微藻的平板式光生物反应器高密度培养;许波等;《食品与发酵工业》;20030131;第29卷(第1期);2.1节和图2-3，36页第1段 * |
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