CN102525685A - Control method for synchronization of menstrual cycles of donor mare and receptor mare - Google Patents
Control method for synchronization of menstrual cycles of donor mare and receptor mare Download PDFInfo
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- CN102525685A CN102525685A CN2012100257914A CN201210025791A CN102525685A CN 102525685 A CN102525685 A CN 102525685A CN 2012100257914 A CN2012100257914 A CN 2012100257914A CN 201210025791 A CN201210025791 A CN 201210025791A CN 102525685 A CN102525685 A CN 102525685A
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Abstract
The invention belongs to the technical field of embryo transplantation and relates to a control method for synchronization of menstrual cycles of a donor mare and a receptor mare. The control method comprises the steps of respectively examining the donor mare and the receptor mare more than once every day, and recording dynamic information of oestrus states, follicle growth states and ovulation time; making a form for the dynamic information to track, observe and record menstrual cycle information of the maria; using prostaglandin on the donor mare and the receptor mare to enable the maria to be in oestrum according to difference with the dynamic information; measuring the diameters of follicles according to the dynamic information and images after the maria are in oestrum; finally utilizing human chorionic gonadotropin to promote ovulation and generate corpus luteum according to physiological difference; and achieving synchronization of menstrual cycles of the donor mare and the receptor mare. The control method is simple in principle, easily controllable in process, accurate in control, high in efficiency and good in effect.
Description
Technical field:
The invention belongs to horse embryo engineering technical field, relate to a kind of confession, synchronous method of receptor mare physiological period controlled, when being used for that horse carried out embryo transfer, making and supply with ovum and be in the same physiology estrus cycle with the mare of accepting ovum.
Background technology:
The estrus synchronization of control animal is the new technique that control is oestrused in a kind of embryo transfer; Be meant that medicines such as adopting hormone breaks the spontaneous estrus rule of female animal and let several female animals occur the state of oestrusing simultaneously; This method is absolutely necessary in the embryo transfer process; Must guarantee that the physiological period of donor female animal and receptor female animal is synchronous, so that transplant and embryo nidation.In the embryo transfer process of domestic animals such as cattle, sheep, after usually the donor female animal being surpassed row and handles, the receptor female animal concentrated carry out estrus synchronization and handle, make receptor female animal estrus synchronization and concentrate ovulation, be convenient to the carrying out of embryo transfer.Equus is longer oestrus, and be not to wait in 4-7 days and animal that individual variation is bigger general oestrus, is difficult to guarantee it behind the estrus synchronization of horse and concentrates ovulation that therefore present general synchronization of Estrus method is difficult to guarantee that the physiological period of mare is synchronous.In addition, the follicular development of equus has uncontrollability, is difficult to realize superovulation.Average each the estrus cycle number of eggs ovulated of mare is 1.2, and this makes that general female animal synchronization of Estrus is being applied to lose effectiveness aspect the equus in the prior art.Utilize ultrasound wave B ultrasonic diagnostic apparatus, observe mare uterus and ovary, accurately grasp the physiological status of every mare; According to supply, receptor physiology gap, select to oestrus control or ovulation control, or oestrus simultaneously and ovulate control; Thereby accurate more regulation and control supply, the physiological period of receptor mare; Synchronizing them, for the embryo transfer condition that provides safeguard, is the basic technology content in the animal rearing new variety technology.
Summary of the invention:
The objective of the invention is to overcome the shortcoming that prior art exists, seek to design a kind of to supply, the receptor mare oestrus and ovulatory cycle is intervened and is controlled, reach same physiological environment in the hope of making confession, receptor; For embryo transfer work lays the foundation, utilize ultrasound wave B ultrasonic diagnostic apparatus to observe mare uterus and ovary, accurately grasp the physiological status of every mare; According to confession, receptor physiology gap; Selection is oestrused and is controlled or ovulation control, or the control of oestrusing simultaneously and ovulate, thus the physiological period of accurate more regulation and control confession, receptor mare; Synchronize them, be the embryo transfer condition that provides safeguard.
To achieve these goals, the present invention realizes through following steps:
Earlier uterus and the ovary of confession, receptor mare are checked respectively with the B ultrasonic diagnostic apparatus that (1) be examined mare and get into more than the post-estrus every day of the inspection once, record is examined the multidate information of the state of oestrusing, ovarian follicular growth state and the ovulation period of mare;
(2) multidate information with every mare ultrasound diagnosis gathers into table, the physiological period information of tracing observation and every mare of record;
(3) according to supply, physiological period information and the difference thereof of receptor mare; In pmgravid 5-14 days of mare; Choose the right moment donor or receptor mare are used prostaglandin (PG); The using dosage of PG be 0.2mg//inferior, method is intramuscular injection, use PG after 1-2 days mares promptly get into oestrus; Or reuse PG next day, and shorten luteal phase, impel and oestrus;
(4) supplying the receptor mare to get into post-estrus,, measuring FD according to the multidate information and the image that carry out B ultrasonic detection gained every day;
(5) according to supply, the physiology gap of receptor mare, when FD reaches 35mm when above, choosing the right moment promotes ovulation and generates corpus luteum with injection human chorionic gonadotropin (HCG) donor or receptor mare; The using dosage of HCG be 1500 units// inferior, method for using is intravenous injection, uses the back mare in 36-48 hour, to ovulate; The interior meeting of mare body produces antibody after using HCG, and HCG used once in an estrus cycle, in whole sexual season, used to be no more than 3-4 time; Realize supplying the estrus cycle of receptor mare to carry out synchronously.
The present invention compared with prior art, its principle is simple, technical process is prone to control, controlled target is accurate, efficient is high, and is effective, can be in extensive use in the breeding of horse and the embryo transfer technology, raising transplanting succeed rate and survival rate.
The specific embodiment:
Below in conjunction with embodiment the present invention is done and to further describe.
Embodiment 1: the control when oestrusing of receptor mare lagged behind the donor mare.The concrete steps of present embodiment comprise:
(1) elder generation checks respectively with the B ultrasonic diagnostic apparatus to uterus and the ovary of confession, receptor mare, is examined mare and gets into more than the post-estrus every day of the inspection once, and record is examined the state of oestrusing, ovarian follicular growth state and the ovulation period of mare;
(2) the ultrasound diagnosis result with every mare gathers into table, so that follow the tracks of the physiological period of every mare;
(3) according to supply, the physiology gap of receptor mare, in pmgravid 5-14 days of mare, choose the right moment; Donor or the injection of receptor mare are used prostaglandin (PG); The using dosage of PG be 0.2mg//inferior, method is intramuscular injection, use PG after 1-2 days mares can oestrus; Or reuse PG next day; Shorten luteal phase, impel and oestrus;
(4) supply the receptor mare to get into post-estrus,, measure FD according to carrying out the image that B ultrasonic detects every day;
(5) according to supply, the physiology gap of receptor mare, when FD reaches 35mm when above, choose the right moment, donor or receptor mare are promoted ovulation and generate corpus luteum with injection human chorionic gonadotropin (HCG); The using dosage of HCG be 1500 units// inferior, method for using is intravenous injection, uses the back mare in 36-48 hour, to ovulate; The interior meeting of mare body produces antibody after using HCG, so HCG not reproducible use in an estrus cycle, and in whole sexual season, using can not be above 3-4 time; Realize supplying the estrus cycle of receptor mare to carry out the control when oestrusing of receptor mare lagged behind the donor mare synchronously.
Present embodiment uses common ultrasound wave B ultrasonic diagnostic apparatus that donor mare A and receptor mare B are carried out uterus and ovary inspection by above-mentioned steps every day, judges and the physiological status of record A, B, according to the physiology gap of A, B choose the right moment use PG or HCG; For ease of statement, it is the 1st that donor A is begun to show the date stamp of oestrusing, and concrete multidate information is following:
The 1st, FD 25mm was oestrused in donor A performance; Receptor B was in corpus luteum the 6th day;
The 2nd, donor A oestrused obviously, FD 28mm; Receptor B was in corpus luteum the 7th day, to receptor B intramuscular injection 0.2mgPG;
The 3rd, donor A oestrused obviously, FD 32mm; FD 25mm is oestrused in receptor B performance;
The 4th, donor A oestrused obviously, FD 38mm; FD 28mm is oestrused in receptor B performance;
The 5th, donor A oestrused obviously, FD 40mm; Receptor B oestruses obviously, FD 30mm;
The 6th, donor A oestrused obviously, FD 40mm, the obvious thickening of ovarian follicle wall; Receptor B oestruses obviously, FD 34mm;
The 7th day, donor A ovulation; Receptor B oestruses obviously, and FD 37mm injects 1500 HCG of unit to receptor B;
The 8th, donor A was in next corpus luteum the 1st day; Receptor B oestruses obviously, FD 40mm, the obvious thickening of ovarian follicle wall;
The 9th, donor A was in next corpus luteum the 2nd day; Receptor B ovulation, realized confession, receptor mare physiological period synchronously, be the embryo transfer work of getting ready;
Embodiment 2: the control when oestrusing of donor mare lagged behind the receptor mare.The concrete steps of present embodiment are with embodiment 1, use ultrasound wave B ultrasonic diagnostic apparatus that donor mare C, receptor mare D are carried out uterus and ovary inspection every day, judge and the physiological status of record C, D, choose the right moment according to the physiology gap of C, D and use PG or HCG; For ease of statement, it is the 1st that receptor D is begun to show the date stamp of oestrusing, and its multidate information is following:
The 1st, donor C was in corpus luteum the 4th day; FD 23mm is oestrused in receptor D performance;
The 2nd, donor C was in corpus luteum the 5th day; Receptor D oestruses obviously, FD 28mm.To donor C intramuscular injection 0.2mgPG;
The 3rd, donor C did not oestrus as yet; Receptor D oestruses obviously, and FD 32mm is to donor C intramuscular injection 0.2mgPG once more;
The 4th, FD 28mm was oestrused in donor C performance; Receptor D oestruses obviously, FD 38mm;
The 5th, donor C oestrused obviously, FD 32mm; Receptor D oestruses obviously, FD 39mm;
The 6th, donor C oestrused obviously, FD 35mm; Receptor D oestruses obviously, FD 40mm.Donor C is injected 1500 HCG of unit;
The 7th, donor C oestrused obviously, FD 38mm; Receptor D oestruses obviously, FD 40mm, ovarian follicle wall thickening;
The 8th day, donor C ovulation; Receptor D ovulation, realized confession, receptor mare physiological period synchronously;
The control procedure of present embodiment has realized that the estrus cycle of confession, receptor mare carries out synchronously, proves that method of the present invention has possessed application conditions, and the control in the practice is respond well, can apply fully.
Claims (1)
1. a confession, the synchronous control method of receptor mare physiological period is characterized in that realizing through following steps:
Earlier uterus and the ovary of confession, receptor mare are checked respectively with the B ultrasonic diagnostic apparatus that (1) be examined mare and get into more than the post-estrus every day of the inspection once, record is examined the multidate information of the state of oestrusing, ovarian follicular growth state and the ovulation period of mare;
(2) multidate information with every mare ultrasound diagnosis gathers into table, the physiological period information of tracing observation and every mare of record;
(3) according to supply, physiological period information and the difference thereof of receptor mare; In pmgravid 5-14 days of mare; Choose the right moment donor or receptor mare are used prostaglandin or claim PG; The using dosage of PG be 0.2mg//inferior, method is intramuscular injection, use PG after 1-2 days mares promptly get into oestrus; Or reuse PG next day, and shorten luteal phase, impel and oestrus;
(4) supplying the receptor mare to get into post-estrus,, measuring FD according to the multidate information and the image that carry out B ultrasonic detection gained every day;
(5) according to supply, the physiology gap of receptor mare, when FD reaches 35mm when above, choose the right moment to donor or receptor mare with the injection human chorionic gonadotropin or claim that HCG promotes ovulation and generates corpus luteum; The using dosage of HCG be 1500 units// inferior, method for using is intravenous injection, uses the back mare in 36-48 hour, to ovulate; The interior meeting of mare body produces antibody after using HCG, and HCG used once in an estrus cycle, in whole sexual season, used to be no more than 3-4 time; Realize supplying the estrus cycle of receptor mare to carry out synchronously.
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| CN2012100257914A CN102525685A (en) | 2012-02-07 | 2012-02-07 | Control method for synchronization of menstrual cycles of donor mare and receptor mare |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110378888A (en) * | 2019-07-22 | 2019-10-25 | 新名医(北京)科技有限公司 | A kind of physiology phase monitoring method, device, ultrasonic device and storage medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110378888A (en) * | 2019-07-22 | 2019-10-25 | 新名医(北京)科技有限公司 | A kind of physiology phase monitoring method, device, ultrasonic device and storage medium |
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Application publication date: 20120704 |