CN102525570A - Sample collector - Google Patents
Sample collector Download PDFInfo
- Publication number
- CN102525570A CN102525570A CN2011104455750A CN201110445575A CN102525570A CN 102525570 A CN102525570 A CN 102525570A CN 2011104455750 A CN2011104455750 A CN 2011104455750A CN 201110445575 A CN201110445575 A CN 201110445575A CN 102525570 A CN102525570 A CN 102525570A
- Authority
- CN
- China
- Prior art keywords
- sample
- collecting device
- liquid
- sample collecting
- matrix structure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007788 liquid Substances 0.000 claims abstract description 110
- 238000001514 detection method Methods 0.000 claims abstract description 56
- 239000000463 material Substances 0.000 claims abstract description 36
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 28
- 210000004369 blood Anatomy 0.000 claims abstract description 11
- 239000008280 blood Substances 0.000 claims abstract description 11
- 210000003608 fece Anatomy 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims description 41
- 238000010521 absorption reaction Methods 0.000 claims description 27
- 229920006395 saturated elastomer Polymers 0.000 claims description 25
- -1 polyethylene Polymers 0.000 claims description 15
- 238000007654 immersion Methods 0.000 claims description 10
- 230000001186 cumulative effect Effects 0.000 claims description 9
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 8
- 206010061926 Purulence Diseases 0.000 claims description 7
- 230000028327 secretion Effects 0.000 claims description 7
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 229920001903 high density polyethylene Polymers 0.000 claims description 6
- 239000004700 high-density polyethylene Substances 0.000 claims description 6
- 229920000728 polyester Polymers 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 4
- 239000004677 Nylon Substances 0.000 claims description 3
- 239000002033 PVDF binder Substances 0.000 claims description 3
- 239000004695 Polyether sulfone Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 229950000845 politef Drugs 0.000 claims description 3
- 229920006393 polyether sulfone Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 238000003317 immunochromatography Methods 0.000 abstract description 12
- 239000000126 substance Substances 0.000 abstract description 10
- 241001465754 Metazoa Species 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 2
- 238000005070 sampling Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 description 19
- 238000002360 preparation method Methods 0.000 description 17
- 230000000694 effects Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- NDAUXUAQIAJITI-UHFFFAOYSA-N albuterol Chemical compound CC(C)(C)NCC(O)C1=CC=C(O)C(CO)=C1 NDAUXUAQIAJITI-UHFFFAOYSA-N 0.000 description 10
- 229960002052 salbutamol Drugs 0.000 description 10
- 239000002131 composite material Substances 0.000 description 9
- 239000002904 solvent Substances 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000004587 chromatography analysis Methods 0.000 description 6
- 210000000214 mouth Anatomy 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000005484 gravity Effects 0.000 description 4
- 210000004877 mucosa Anatomy 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 210000003679 cervix uteri Anatomy 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000005030 aluminium foil Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- YJQZYXCXBBCEAQ-UHFFFAOYSA-N ractopamine Chemical compound C=1C=C(O)C=CC=1C(O)CNC(C)CCC1=CC=C(O)C=C1 YJQZYXCXBBCEAQ-UHFFFAOYSA-N 0.000 description 2
- 229940074095 ractopamine Drugs 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 239000011800 void material Substances 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010073310 Occupational exposures Diseases 0.000 description 1
- 206010037742 Rabies Diseases 0.000 description 1
- 241000702670 Rotavirus Species 0.000 description 1
- 206010046555 Urinary retention Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000013070 direct material Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000675 occupational exposure Toxicity 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a sample collector. The sample collector comprises a handle part, a board part and a combining part connected with the handle part and the board part. The sample collector is made from a hydrophobic material. The hydrophobic material forms a capillarity micropore substrate structure. The capillarity micropore substrate structure is used for collecting a liquid sample and automatically separating and conveying target detection substances of the liquid sample. The sample collector disclosed by the invention has the advantages of low production cost, capability of sampling liquid or semi-liquid samples, such as secreted fluid, residual liquid or feces from natural short orifice of human body and living animals, blood on wound surfaces, purulent or non-purulent secreted fluid and the like, capability of automatically separating and conveying target detection substances of liquid sample and capability of rapidly detecting the target detection substances by combining with an immunochromatography or other detecting technology.
Description
Technical field
The present invention relates to a kind of sample collecting device, relate in particular to that a kind of cost is low, range of application sample collecting device widely.
Background technology
Blood (comprising whole blood, serum or blood plasma) be widely used clinically as the health detection sample, but its gatherer process must puncture human or animal's physical barriers---skin, not only causes secondary infection easily, and be the principal element that forms various occupational exposure.And various natural short cavities road (the Natural Short Cavity; NSC) like mouth, eye, ear, nose, throat, urogenital tract (cervix uteri-vagina), lower digestive tract (rectum-anus) etc.; All types of target detects thing and blood occurs having concordance opportunity mostly in liquid state such as its juice, debris or feces or the semi liquid state sample, only aspect content, there are differences.The result of study of Challacombe confirms the IgG existence of the interior IgG of antimer in time in the mouth cavity liquid; Eshaq etc. carry out Ractopamine and have confirmed the regularity of distribution of Ractopamine in mouth cavity liquid, urethral orifice residual urine in the research of live body pig internal metabolism rule; And some infectious disease itself also is through the external toxin expelling in natural short cavity road, like the feces of saliva, rotavirus and the adenovirus infant of rabies morbidities animal etc., and becomes the main source of infection or the route of transmission of these diseases.In addition, the collection of natural short cavity road liquid sample often can not form new wound.So, along with the further investigation of natural short cavity road liquid sample acquisition technique, certainly will reform existing Clinical detection pattern, bring up large quantities of new detection techniques and testing product.
Wherein, Patent ZL200710169735.7 discloses a kind of sample collecting device of gathering the oral cavity liquid sample; Process by hydrophilic fibre and hydrophobic material, form capillary micropore matrix structure, its liquid sample that contains the target detection thing that suits to be applied to gather; And separate voluntarily and carry the target detection thing, be particularly useful for macromole target detection thing.But; To micromolecule target detection thing; Like glucose, ethanol, residual chemicals etc.; Its separate voluntarily and conveying capacity not as good as to the effect of macromole target detection thing, like protein, nucleic acid or polysaccharide etc., though therefore the sample collecting device of this invention is not an optimal choice using aspect the separation voluntarily of micromolecule target detection thing and the conveying effect.Simultaneously, with the sample collecting device of Composite Preparation, its direct material cost is higher; And different component is completely different aspect physics and chemical property; Cause preparation and quality control institute requirement difference huge, necessarily require strict its working condition of control and technological process, but still occur substandard product easily; Make production cost high, seriously hinder the research and development and the marketing of the subsequent applications product of this sample collecting device.
Patent ZL201020665818.2 discloses a kind of nanoscale sample collecting device and checkout gear thereof, and its core is that the aperture is in the hydrophobic material sample collecting device between 50～900 nanometers.But the nanoscale sample collecting device of this hydrophobic material preparation is because the aperture is too small, and viscous force increases; Transporting velocity is slow; And in the hydrophobic material of nanoscale aperture, the capillarity direction is identical with gravity direction, and along with the aperture dwindles and active force is strong more; That is to say that the liquid level in this sample collecting device is lower than the liquid level of sample collecting device outside; Be that this sample collecting device needs the effect of external force could realize the contrary gravity direction conveying of liquid sample, can't realize upwards carrying of liquid sample, promptly can't realize conveying effect voluntarily by the capillarity of material itself.
Summary of the invention
The object of the present invention is to provide a kind of sample collecting device; Production cost is low; Can be applicable to people and living animal nature short cavity road (the Natural Short Cavity, the NSC) collection of liquid state such as juice, debris, feces and wound surface blood, purulence or non-purulent secretion liquid or semi liquid state sample, and separate voluntarily and carry target detection thing in the liquid sample; Through combining, realize the fast detecting of target detection thing with detection technique such as immunochromatography.
For realizing above-mentioned purpose; The present invention provides a kind of sample collecting device, comprising: shank, board and the joint portion that connects shank and board, and said sample collecting device is processed by hydrophobic material; This hydrophobic material forms capillary micropore matrix structure (the Capillary Micro-Aperture Basic Material Texture; CMBM), this capillary micropore matrix structure is used for liquid sample is gathered, and also is used for separating voluntarily and carries liquid sample target detection thing.
Said hydrophobic material is one or more in polyethylene, polypropylene, multi-styrene, high density polyethylene (HDPE), ultra high molecular polyethylene, Polyvinylidene fluoride, politef, polyester, nylon, polyether sulfone and other plastics, glass, the resin, the preferred polyester material.
Said sample collecting device profile is bar-shaped or tabular, and the sample collecting device cumulative volume is not less than 650 cubic millimeters, and total surface area is not less than 700 square millimeters, and wherein the surface area of board is not less than 50% of sample collecting device total surface area.
The preferred tabular oar shape of said sample collecting device profile.
Said capillary micropore matrix structure is netted microcellular structure; Its aperture is 20～500 microns; Voidage is 5～80%; The relative density of sample collecting device is 0.49～0.92 gram/cubic centimetre, and capillary micropore matrix structure saturated absorption amount of liquid is 50～550 cubic millimeters, is no more than 5 minutes with the immersion way saturated absorption liquid time.
Preferred 20～200 microns of the aperture of said capillary micropore matrix structure; Voidage preferred 35～55%; Preferred 0.58～0.72 gram/cubic centimetre of the density of sample collecting device; Capillary micropore matrix structure saturated absorption amount of liquid is preferably 200～400 cubic millimeters, preferably is no more than 1 minute with the immersion way saturated absorption liquid time.
The dry again back of capillary micropore matrix structure saturated absorption liquid sample is compared with former sample harvester, its cumulative volume, total surface area, aperture, voidage, saturated absorption amount of liquid, is no more than 20% with the rate of change of immersion way saturated absorption liquid time parameter numerical value.
The dry again back of capillary micropore matrix structure saturated absorption liquid sample is compared with former sample harvester, its cumulative volume, total surface area, aperture, voidage, saturated absorption amount of liquid, preferably is no more than 1% with the rate of change of immersion way saturated absorption liquid time parameter numerical value.
Do not having under extraneous mechanical compress or the filtering prerequisite, be adsorbed in the intramatrical liquid sample of capillary micropore is transported to the shank edge from the board edge time to be controlled in 10 minutes, preferably be no more than 2 minutes.
The hydrophobic material of said sample collecting device or making sample collecting device can pass through hydrophilic treated, with the ability that improves the sample collecting device collection and carry liquid sample.
Said liquid sample comprises natural short cavity road juice, debris, feces and wound surface blood, purulence or the non-purulent secretion liquid etc. of liquid state or semi liquid state, and natural short cavity road comprises mouth, eye, ear, nose, throat, urogenital tract (cervix uteri-vagina), lower digestive tract (rectum-anus) etc.
Beneficial effect of the present invention: sample collecting device of the present invention; Process by hydrophobic material; Its capillary micropore matrix structure not only has liquid state or semi liquid state liquid sample acquisition function; Also have to separate voluntarily and carry and (only rely on the capillarity that sample collecting device itself possesses; Need not extraneous mechanical compress or applied external force such as centrifugal or gravity) effect of target detection thing in the liquid sample; Through combining with detection technique such as immunochromatography, realize the fast detecting of target detection thing in liquid state such as people and the natural short cavity of living animal road juice, debris, feces and wound surface blood, purulence or non-purulent secretion liquid or the semi liquid state liquid sample, have painless safer, the simple to operate more easy-to-use characteristics of noinvasive.And cost of the present invention is low, and it is high to produce qualification rate in batches, can promote that the present invention is widely used in Clinical detection.In addition; Be directed against micromolecule target detection thing; Such as glucose, ethanol and residual chemicals etc., it separates voluntarily and the conveying effect is better than the sample collecting device of Composite Preparation, also is applicable to macromole target detection thing simultaneously; Such as protein, nucleic acid or polysaccharide etc., and satisfy the Clinical detection needs.
Description of drawings
Below in conjunction with accompanying drawing, describe in detail through specific embodiments of the invention, will make technical scheme of the present invention and other beneficial effects obvious.
In the accompanying drawing,
Fig. 1 is the structural representation of sample collecting device of the present invention.
The specific embodiment
Describe the present invention below in conjunction with accompanying drawing.
As shown in Figure 1, sample collecting device of the present invention comprises shank 2, board 4 and the joint portion 6 that connects shank 2 and board 4; Sample collecting device is processed by hydrophobic material; This hydrophobic material forms capillary micropore matrix structure (the Capillary Micro-Apeture Basic Material Texture; CMBM); This capillary micropore matrix structure is used for liquid sample is gathered, and also is used for separating voluntarily and carrying the target detection thing of liquid sample.Described liquid sample comprises the people and living animal nature short cavity road (the Natural Short Cavity of liquid state or semi liquid state; NSC) juice, debris, feces and wound surface blood, purulence or non-purulent secretion liquid etc., natural short cavity road comprise mouth, eye, ear, nose, throat, urogenital tract (cervix uteri-vagina), lower digestive tract (rectum-anus) etc.Said hydrophobic material is one or more in polyethylene, polypropylene, multi-styrene, high density polyethylene (HDPE), ultra high molecular polyethylene, Polyvinylidene fluoride, politef, polyester, nylon, polyether sulfone and other plastics, glass, the resin etc., the hydrophobic material preferred polyester.
No direct correlation property between the profile of said sample collecting device and the collection effect, its profile can be but be not limited to bar-shaped or tabular, in the present embodiment, the preferred tabular oar shape of sample collecting device profile.The sample collecting device cumulative volume is not less than 650 cubic millimeters, and total surface area is not less than 700 square millimeters, and the surface area of the board 4 that wherein directly contacts with natural short cavity road mucosa or wound surface is not less than 50% of sample collecting device total surface area.
Said capillary micropore matrix structure is netted microcellular structure, and the parameter of capillary micropore matrix structure is extremely crucial for the performance of sample collecting device, and capillary micropore matrix structure itself must be kept the less size that compares; Specifically; The micropore size of capillary micropore matrix structure should be controlled at certain scope, and its aperture is 20～500 microns, preferred 20～200 microns; Purpose is to prevent that the stickum in the liquid sample is blocking microporous; Improve transfer efficiency, but the non-target detection thing of some macromole in the filter liquide sample also simultaneously lets the target detection thing in the liquid sample preferentially pass through; And arrive at the detection part of combining closely with sample collecting device shank 2; Effectively improve the front end concentration of target detection thing in solvent, make the loss in conveying and testing process of target detection thing minimum, also can obviously not change capillary micropore matrix structure in addition for the assurance liquid sample.The voidage of capillary micropore matrix structure (being the total measurement (volume) of all micropores of capillary micropore substrate and the ratio of sample collecting device cumulative volume) is 5～80%; Preferred 35～55%; Purpose is the liquid sample that had both guaranteed to collect q.s; Guarantee that again the liquid sample gathered can be transported to detection part fully, make that the residual quantity of target detection thing reaches lower level in the capillary micropore matrix structure.The relative density of sample collecting device is 0.49～0.92 gram/cubic centimetre, preferred 0.58～0.72 gram/cubic centimetre.
Said sample collecting device is 50～550 cubic millimeters through its capillary micropore matrix structure saturated absorption amount of liquid, is preferably 200～400 cubic millimeters, is no more than 5 minutes with the immersion way saturated absorption liquid time, preferably is no more than 1 minute.The profile of sample collecting device and inner capillary micropore matrix structure be not because of the obvious change that reached capacity of the absorption to liquid sample; Be the absorption liquid sample body to the profile of sample collecting device and volume all less than significantly influence; Can not destroy a mao minute aperture matrix structure significantly yet; Also can not influence the total void rate of mao minute aperture matrix structure pore size and capillary micropore matrix structure significantly, and through after the dried, tangible change does not take place in its speed that absorbs liquid once more.For satisfying above-mentioned requirements, sample collecting device and capillary micropore matrix structure should have certain rigid or hardness, but its hardness can not produce great damage to natural short cavity road mucosa or wound surface when the collection liquid sample; The cumulative volume of the hair minute aperture matrix structure that meets the demands, total surface area, aperture, voidage, saturated absorption amount of liquid, with the numerical value of immersion way saturated absorption liquid time parameter at sample collecting device saturated absorption liquid sample again after the drying; The change rate is no more than 20%, preferably is no more than 1%.
The material of sample collecting device form with and the parameter (as: difference of the total void rate of the pore size of capillary micropore matrix structure and capillary micropore matrix structure) of the internal structure capillary micropore matrix structure time of delivery of liquid sample on sample collecting device that determined the collection capacity of liquid sample and gathered.Do not having under extraneous mechanical compress or the filtering prerequisite; Being adsorbed in liquid sample in the capillary micropore matrix structure is transported to shank 2 edges voluntarily from board 4 edges and (only relies on the capillarity that sample collecting device itself possesses; Need not extraneous mechanical compress or applied external force such as centrifugal or gravity; It carries air line distance is 45mm) time be controlled in 10 minutes, preferably in 2 minutes.
The hydrophobic material that above sample collecting device is perhaps made sample collecting device also can pass through hydrophilic treated, with the ability that improves the sample collecting device collection and carry liquid sample.
Sample collecting device of the present invention is realized the fast detecting of target detection thing through combining with detection techniques such as including but not limited to immunochromatography.Can be used in combination with various detectable bars; During use; Board 4 is used to extend into nature short cavity road and contacts its mucosa or directly contact wound surface, scrapes lightly and gets liquid sample, and shank 2 is used for the joint detection reagent strip; Detectable bar utilization immunological technique and chromatographic theory detect and obtain testing result to the target detection thing in the liquid sample.Specifically; The capillary micropore matrix structure that sample collecting device forms through its hydrophobic material; Can fully gather nature short cavity road mucosa or wound surface liquid sample, need not extraneous mechanical compress and can separate and carry the target detection thing in institute's collection liquid sample voluntarily, in conjunction with detectable bar with utilization immunological technique and chromatographic theory; Be transported to the sample application zone of detectable bar when effective liquid sample; Through reagent strip immunochromatography principle, make liquid sample and target detection thing wherein be transported to the reagent strip reaction zone again, a series of biology, chemical reaction take place at this reaction zone; And demonstrate chromatic band in detection zone and check plot (or Quality Control district) of reaction zone respectively, can read testing result in view of the above.
Below be effect that the embodiment of the invention is applied to Clinical detection relatively; Promptly make up the sample collecting device of hydrophobic material preparation of the present invention or sample collecting device and the HIV-1/2 antibody immune chromatography reagent strip (application examples 1) or the albuterol immunochromatography reagent bar (application examples 2) of the disclosed Composite Preparation of ZL200710169735.7 patent of invention respectively; Detect HIV antibody (macromole target detection thing) and albuterol (micromolecule target detection thing) respectively; And it is compared with directly using testing result of HIV-1/2 antibody immune chromatography reagent strip or albuterol immunochromatography reagent bar, purpose is to set forth creativeness of the present invention and meaning thereof.
Application examples 1:
1) test material:
300 parts of HIV-1/2 antibody immune chromatography reagent strips (self-control, lot number 2011062301), HIV-1 positive serum 5mL, BSA serum dilution 200mL, developing solvent.
2) test method:
(1) sample collecting device of 100 parts of HIV-1/2 antibody immune chromatography reagent strips and the disclosed Composite Preparation of ZL200710169735.7 is formed sample B with its said mode; The sample collecting device of 100 parts of HIV-1/2 antibody immune chromatography reagent strips and hydrophobic material of the present invention preparation is formed sample C with mode according to the invention; Remaining 100 parts of HIV-1/2 antibody immune chromatography reagent strips is Sample A; Use packaging of aluminium foil bag respectively, seal shady and cool room temperature preservation, subsequent use.
(2) get an amount of HIV-1 positive serum with serum dilution with * 20, * 50, * 100, * 200, * 500, * 1000, * 2000, * 5000, * 10000, * 20000 gradient dilutions are subsequent use.
(3) get the HIV-1 serum after the 25uL dilution with pipettor, drop to the sample collecting device board 4 of Sample A sample application zone or sample B and C respectively, again in the sample collecting device insertion developing solvent with Sample A sample application zone or sample B and C, read testing result in 10-20 minute.
3) result of the test:
4) interpretation of result:
It is suitable that sample B and sample C detect effect to the HIV-1 serum of variable concentrations, no significant difference, but obviously be superior to Sample A, and significant difference.Explain that the sample collecting device of hydrophobic material preparation of the present invention and the sample collecting device of the disclosed Composite Preparation of ZL200710169735.7 patent of invention are directed against macromole target detection thing; Has equal effect; And has an effect of the macromole target detection thing that separates voluntarily in institute's collection liquid sample; Make in conveying and testing process, improved the front end concentration of target detection thing in solvent (comprising liquid sample and developing solvent) effectively.In addition; Employed HIV-1/2 antibody reagent bar is the high sensitivity reagent strip in this test; HIV-1 serum also is strong positive; The sample collecting device of hydrophobic material of the present invention preparation and the sample collecting device of the disclosed Composite Preparation of ZL200710169735.7 patent of invention are not only sacrificed specificity to the high sensitivity reagent strip, also because effectively block other impurity through and improved the specificity of reagent strip indirectly.
Application examples 2:
1) test material:
210 parts of albuterol immunochromatography reagent bars (self-control, lot number 2011100601), albuterol urine standard substance (national residue of veterinary drug benchmark laboratory provides), developing solvent.
2) test method:
(1) sample collecting device of 70 parts of albuterol immunochromatography reagent bars and the disclosed Composite Preparation of ZL200710169735.7 is formed sample E with its said mode; The sample collecting device of 70 parts of albuterol immunochromatography reagent bars and hydrophobic material according to the invention preparation is formed sample F with mode according to the invention; Remaining 70 parts of albuterol immunochromatography reagent bars is sample D; Use packaging of aluminium foil bag respectively, seal shady and cool room temperature preservation, subsequent use.
(2) respectively Dropwise 5 0uL variable concentrations albuterol urine standard substance again in the sample collecting device insertion developing solvent with the sample application zone of sample D or sample E and F, were read testing result to the sample collecting device board 4 of sample D sample application zone or sample E and F in 10-20 minute.
3) result of the test:
4) interpretation of result:
Sample F obviously is superior to sample E and sample D to the detection effect of the albuterol standard substance of variable concentrations, and sample E is superior to sample D, and all difference is remarkable.The Clinical detection of the sample collecting device of hydrophobic material preparation of the present invention to micromolecule target detection thing is described; Its voluntarily separating property be superior to the sample collecting device of the disclosed Composite Preparation of ZL200710169735.7 patent of invention; Make in conveying and the testing process, improved the front end concentration of target detection thing in solvent (comprising liquid sample and developing solvent) more effectively.
In sum; Sample collecting device of the present invention; Process by hydrophobic material; Its capillary micropore matrix structure not only has liquid state or semi liquid state liquid sample acquisition function, also has the effect that separates voluntarily and carry target detection thing in the liquid sample, through combining with detection techniques such as immunochromatographies; Realize the fast detecting of target detection thing in liquid state such as people and the natural short cavity of living animal road juice, debris, feces and wound surface blood, purulence or non-purulent secretion liquid or the semi liquid state liquid sample, have painless safer, the simple to operate more easy-to-use characteristics of noinvasive.And cost of the present invention is low, and it is high to produce qualification rate in batches, can promote liquid state such as nature short cavity road juice, debris, feces and wound surface blood, purulence or non-purulent secretion liquid or semi liquid state liquid sample to be widely used in Clinical detection.In addition; Be directed against micromolecule target detection thing; Such as glucose, ethanol and residual chemicals etc., it separates voluntarily and the conveying effect is better than the sample collecting device of Composite Preparation, also is applicable to macromole target detection thing simultaneously; Such as protein, nucleic acid or polysaccharide etc., and satisfy the Clinical detection needs.
The above for the person of ordinary skill of the art, can make other various corresponding changes and distortion according to technical scheme of the present invention and technical conceive, and all these changes and distortion all should belong to the scope that the present invention protects.
Claims (10)
1. sample collecting device; It is characterized in that; Comprise: shank, board and the joint portion that connects shank and board, said sample collecting device is processed by hydrophobic material, and this hydrophobic material forms capillary micropore matrix structure; This capillary micropore matrix structure is used for liquid sample is gathered, and also is used for separating voluntarily and carries liquid sample target detection thing.
2. sample collecting device as claimed in claim 1; It is characterized in that said hydrophobic material is one or more in polyethylene, polypropylene, multi-styrene, high density polyethylene (HDPE), ultra high molecular polyethylene, Polyvinylidene fluoride, politef, polyester, nylon, polyether sulfone and other plastics, glass, the resin.
3. sample collecting device as claimed in claim 1 is characterized in that, said hydrophobic material preferred polyester material.
4. sample collecting device as claimed in claim 1; It is characterized in that; Said sample collecting device profile is bar-shaped or tabular; The sample collecting device cumulative volume is not less than 650 cubic millimeters, and total surface area is not less than 700 square millimeters, and wherein the surface area of board is not less than 50% of sample collecting device total surface area.
5. sample collecting device as claimed in claim 1; It is characterized in that said capillary micropore matrix structure is netted microcellular structure, its aperture is 20～500 microns; Voidage is 5～80%; The relative density of sample collecting device is 0.49～0.92 gram/cubic centimetre, and capillary micropore matrix structure saturated absorption amount of liquid is 50～550 cubic millimeters, is no more than 5 minutes with the immersion way saturated absorption liquid time.
6. sample collecting device as claimed in claim 5; It is characterized in that; Preferred 20～200 microns of the aperture of said capillary micropore matrix structure, voidage is preferred 35～55%, preferred 0.58～0.72 gram/cubic centimetre of the density of sample collecting device; Capillary micropore matrix structure saturated absorption amount of liquid is preferably 200～400 cubic millimeters, preferably is no more than 1 minute with the immersion way saturated absorption liquid time.
7. sample collecting device as claimed in claim 1; It is characterized in that; The dry again back of capillary micropore matrix structure saturated absorption liquid sample is compared with former sample harvester, its cumulative volume, total surface area, aperture, voidage, saturated absorption amount of liquid, is no more than 20% with the rate of change of immersion way saturated absorption liquid time parameter numerical value.
8. sample collecting device as claimed in claim 7; It is characterized in that; The dry again back of capillary micropore matrix structure saturated absorption liquid sample is compared with former sample harvester, its cumulative volume, total surface area, aperture, voidage, saturated absorption amount of liquid, preferably is no more than 1% with the rate of change of immersion way saturated absorption liquid time parameter numerical value.
9. sample collecting device as claimed in claim 1 is characterized in that, is not having under extraneous mechanical compress or the filtering prerequisite, is adsorbed in liquid sample in the capillary micropore matrix structure is transported to the shank edge from the board edge time to be controlled in 10 minutes.
10. sample collecting device as claimed in claim 1; It is characterized in that; Liquid sample comprises natural short cavity road juice, debris, feces and wound surface blood, purulence or the non-purulent secretion liquid etc. of liquid state or semi liquid state, and natural short cavity road comprises mouth, eye, ear, nose, throat, urogenital tract, lower digestive tract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110445575 CN102525570B (en) | 2011-12-27 | 2011-12-27 | Sample collector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110445575 CN102525570B (en) | 2011-12-27 | 2011-12-27 | Sample collector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102525570A true CN102525570A (en) | 2012-07-04 |
| CN102525570B CN102525570B (en) | 2013-09-11 |
Family
ID=46334466
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110445575 Expired - Fee Related CN102525570B (en) | 2011-12-27 | 2011-12-27 | Sample collector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102525570B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136536A (en) * | 2015-08-14 | 2015-12-09 | 江苏恒神股份有限公司 | Composite compression sample manufacturing method |
| CN105223040A (en) * | 2015-09-30 | 2016-01-06 | 中山大学中山眼科中心 | A kind of method of getting tear |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238995A (en) * | 2007-02-05 | 2008-08-13 | 深圳市赛茵斯爱速医疗科技有限公司 | Sample collecting device |
| CN101393213A (en) * | 2007-09-20 | 2009-03-25 | 深圳市赛茵斯爱速医疗科技有限公司 | HIV-1/2 antibody saliva detector |
| CN201955341U (en) * | 2010-12-17 | 2011-08-31 | 苏州万木春生物技术有限公司 | Diagnostic instrument for oral liquid |
-
2011
- 2011-12-27 CN CN 201110445575 patent/CN102525570B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238995A (en) * | 2007-02-05 | 2008-08-13 | 深圳市赛茵斯爱速医疗科技有限公司 | Sample collecting device |
| CN101393213A (en) * | 2007-09-20 | 2009-03-25 | 深圳市赛茵斯爱速医疗科技有限公司 | HIV-1/2 antibody saliva detector |
| CN201955341U (en) * | 2010-12-17 | 2011-08-31 | 苏州万木春生物技术有限公司 | Diagnostic instrument for oral liquid |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105136536A (en) * | 2015-08-14 | 2015-12-09 | 江苏恒神股份有限公司 | Composite compression sample manufacturing method |
| CN105223040A (en) * | 2015-09-30 | 2016-01-06 | 中山大学中山眼科中心 | A kind of method of getting tear |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102525570B (en) | 2013-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11246573B2 (en) | Automated collection and analysis of body fluids | |
| US10690655B2 (en) | Automated urine collector-analyzer | |
| US20100089181A1 (en) | Sample Collection System and Method for Use Thereof | |
| US20140302617A1 (en) | Sample collection system and method for use thereof | |
| US20040018634A1 (en) | Sample-collection and preparation device and method | |
| US20110204084A1 (en) | Sample Collection System and Method for Use Thereof | |
| US10682124B2 (en) | Automated collection and analysis of body fluids | |
| WO2014165184A1 (en) | System for breath sample collection and analysis | |
| CN100539953C (en) | Sample collecting device | |
| CN210534159U (en) | Microfluidic immunofluorescence kit for novel coronavirus excrement detection | |
| US20230098151A1 (en) | Testing for viruses and cellular biomarkers | |
| CN102525570B (en) | Sample collector | |
| US20210196246A1 (en) | Suction Based Saliva Treatment and Collector Device | |
| US20230152192A1 (en) | Filtration sampling devices | |
| JPS62133936A (en) | Apparatus for sampling minute amount of blood | |
| CN208752081U (en) | Feces collection detection device | |
| CN201955341U (en) | Diagnostic instrument for oral liquid | |
| CN101498717B (en) | Sample collecting device used for collecting solid or semi-solid sample | |
| Lee et al. | Urine-sampling methods for environmental chemicals in infants and young children | |
| CN207042502U (en) | A kind of micro-fluidic chip for containing reagent | |
| JP6357473B2 (en) | Plasma separation using a drop of blood | |
| CN202383140U (en) | Rapid Hp antigen/antibody oral cavity detector | |
| Coutinho et al. | Detection of newborn infants at risk for congenitat toxoplasmosis in Rio de Janeiro, Brazil | |
| CN109381222A (en) | A kind of saliva collecting device | |
| CN208319232U (en) | A kind of saliva acquisition suit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HE NANHANG Free format text: FORMER OWNER: SHENZHEN ORAL SAMPLES COLLECTING AND DETECTING TECHNOLOGY INSTITUTE CO., LTD. Effective date: 20150709 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee |
Owner name: SHENZHEN ORAL SAMPLES COLLECTING AND DETECTING TEC Free format text: FORMER NAME: SHENZHEN ISURE BIOTECH CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: Silver Star Technology Building No. 1301 Longhua District Guanlan Street sightseeing road 518000 Guangdong city of Shenzhen Province 7 floor A17 Patentee after: Shenzhen stomatological sample collection and Detection Technology Research Institute Co.,Ltd. Address before: 518000, Guangdong, Shenzhen, Xixiang Baoan District Street science and Technology Innovation Park, the Peach Garden, 4 R & D center, a layer of C.D.E Patentee before: SHENZHEN ISURE BIOTECH Co.,Ltd. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20150709 Address after: Panyu District Qinghe Road Guangzhou city Guangdong province 518000 Luojia No. 88 East Graceland seat seventeen ladder 304 room Patentee after: He Nanhang Address before: Silver Star Technology Building No. 1301 Longhua District Guanlan Street sightseeing road 518000 Guangdong city of Shenzhen Province 7 floor A17 Patentee before: Shenzhen stomatological sample collection and Detection Technology Research Institute Co.,Ltd. |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20120704 Assignee: Guangzhou Bo a biological technology Co.,Ltd. Assignor: He Nanhang Contract record no.: 2015440020378 Denomination of invention: Sample collecting device Granted publication date: 20130911 License type: Exclusive License Record date: 20151221 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Guangzhou Bo a biological technology Co.,Ltd. Assignor: He Nanhang Contract record no.: 2015440020378 Date of cancellation: 20160202 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160418 Address after: 518000 Guangdong, Guangzhou, Zhongshan, No. three road, No. 33, building B, part of the C58 (only for office use) Patentee after: Guangzhou Bo a biological technology Co.,Ltd. Address before: Panyu District Qinghe Road Guangzhou city Guangdong province 518000 Luojia No. 88 East Graceland seat seventeen ladder 304 room Patentee before: He Nanhang |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170303 Address after: 510000 Guangzhou International Biological Island spiral Road No. sixth, layer 601, 602, 603, 604, 605 units 12, three Patentee after: Guangdong dream Biotechnology Co.,Ltd. Address before: 518000 Guangdong, Guangzhou, Zhongshan, No. three road, No. 33, building B, part of the C58 (only for office use) Patentee before: Guangzhou Bo a biological technology Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130911 Termination date: 20181227 |