CN102524317A - Sterilized solution for assay detection instrument - Google Patents
Sterilized solution for assay detection instrument Download PDFInfo
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- CN102524317A CN102524317A CN2012100006121A CN201210000612A CN102524317A CN 102524317 A CN102524317 A CN 102524317A CN 2012100006121 A CN2012100006121 A CN 2012100006121A CN 201210000612 A CN201210000612 A CN 201210000612A CN 102524317 A CN102524317 A CN 102524317A
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Abstract
The invention relates to the field of sterilized solution, in particular to sterilized solution for an assay detection instrument. The sterilized solution is prepared from components in part by weight: dimethyl sulfoxide, polyhexamethylene guanidine, ethanol, sodium dodecyl sulfate, artemisia leaf, indigowoad leaf, ephedra, madder and oriental waterplantain rhizome. The sterilized solution is prepared by the following steps of: adding the artemisia leaves, the indigowoad leaves, the ephedra, the madder and the oriental waterplantain rhizome in boiled water of which the weight is 10 times that of the mixture and decocting for half an hour; filtering and adding filter residue in boiled water of which the weight is 3 times that of the filter residue and decocting for one hour; filtering and combining filtrate collected by two times; condensing the filtrate until the relative density is 1.2; and adding the dimethyl sulfoxide, the polyhexamethylene guanidine, the ethanol and the sodium dodecyl sulfate in the filtrate and uniformly stirring to obtain the sterilized solution. According to the sterilized solution disclosed by the invention, the formula is reasonable; the preparation process is simple; the sterilizing effect is favorable; and the sterilized rate approaches to 100 percent and high popularization and application values are obtained. The sterilized solution is simple in use and easy in operation; high temperature and high pressure are avoided, so that the complexity in operation is reduced; and the sterilized solution is convenient for workers to use and facilitates the popularization.
Description
Technical field
The present invention relates to the thimerosal field, particularly a kind of chemical examination appliance is used sterilized solution.
Background technology
In sanitary inspection quarantine industry; Often need to detect chemical examination virus, detecting virus needs to use utensils such as test tube, glassware, need carry out strict sterilization to these utensils after using; Could reuse; If sterilization was not thorough when utensil used, tend to influence the correctness of testing result, serious even can cause the result with true opposite fully.In regular job, the testing staff uses high-pressure sterilizing pot to sterilize often, is placed on then in storeroom or the dustproof cabinet and preserves.Complex operation uses autoclave appts, also has certain danger.If sterilization effect is bad, in the preservation process, might produce new microorganism in the utensil.How improving the sterilization ability to the chemical examination appliance, and make sterilization process more simple to operation, is the problem that the testing staff need solve.
Summary of the invention
Detect the bad problem of complicated operation, sterilization effect that exists with the utensil sterilization in order to solve above chemical examination, the invention provides and a kind ofly need not the chemical examination appliance that HTHP just can reach fine sterilization ability and use sterilized solution.
The present invention realizes through following measure:
A kind of chemical examination appliance is used sterilized solution, is processed by the component of following weight portion:
Dimethyl sulfoxide (DMSO) 40g, polyhexamethylene guanide 50g, ethanol 200g, dodecyl sodium sulfate 5g, tarragon 20g, folium isatidis 30g, Chinese ephedra 15g, madder 10g, rhizoma alismatis 30g;
The preparation method is following:
Tarragon, folium isatidis, Chinese ephedra, madder, rhizoma alismatis are added in the boiling water of 10 times of weight and decoct half an hour, filter, filter residue adds in the boiling water of 3 times of raw material weights again and decocted 1 hour; Filter; Merge filtrating twice, being concentrated into relative density is 1.2, adds dimethyl sulfoxide (DMSO), polyhexamethylene guanide, ethanol and dodecyl sodium sulfate; Stir, promptly get.
Sterilized solution added in 20-30 ℃ the water, addition is 80-100g/kg.
Beneficial effect of the present invention:
Sterilized solution prescription of the present invention is reasonable, and preparation is simple, and sterilization effect is good, and sterilization rate has popularizing value near 100% very much; Sterilized solution of the present invention uses simple to operation, need not HTHP, has both reduced the input of equipment, has reduced the danger coefficient in the operating process again, reduces the complexity of operation, is convenient to the staff and uses, and is beneficial to popularization.
Embodiment
For a better understanding of the present invention, further specify below in conjunction with specific embodiment.
Embodiment 1:
A kind of chemical examination appliance is used sterilized solution, is processed by the component of following weight portion:
Dimethyl sulfoxide (DMSO) 40g, polyhexamethylene guanide 50g, ethanol 200g, dodecyl sodium sulfate 5g, tarragon 20g, folium isatidis 30g, Chinese ephedra 15g, madder 10g, rhizoma alismatis 30g;
The preparation method is following:
Tarragon, folium isatidis, Chinese ephedra, madder, rhizoma alismatis are added in the boiling water of 10 times of weight and decoct half an hour, filter, filter residue adds in the boiling water of 3 times of raw material weights again and decocted 1 hour; Filter; Merge filtrating twice, being concentrated into relative density is 1.2, adds dimethyl sulfoxide (DMSO), polyhexamethylene guanide, ethanol and dodecyl sodium sulfate; Stir, promptly get.
Sterilized solution added in 20-30 ℃ the water, addition is 80-100g/kg, and sterilization time is 1-1.5 hour.
The sterilization effect test
Chemical examination appliance of the present invention is carried out sterilization effect relatively with sterilized solution and habitual high-pressure sterilizing pot.
1, method of testing:
To after cleaning, be equally divided into two groups with test tube, the culture dish that a test experiment is used; One group of control group uses high-pressure sterilizing pot to sterilize; Another group experimental group uses chemical examination appliance of the present invention to sterilize with sterilized solution, after sterilization finishes, detects the sterilization rate of two groups of utensils.
2, testing procedure:
The control group sterilization steps: test tube, the culture dish that will sterilize are put into high-pressure sterilizing pot, and it is 0.3MPa that operating pressure is set, and operating temperature is 120 ℃, and the sterilization time after the autoclaving pot inner pressure temperature constant is 20min.
Experimental group sterilization steps: the water that in container with cover, injects 2/3 volume; Be warming up to 30 ℃, add chemical examination appliance of the present invention according to the amount of 100g/kg and use sterilized solution, stir; Test tube, the culture dish that to sterilize are then put into container; Be immersed in the liquid, close the lid, soaked 1 hour.
3, test result:
The residual contrast table in table 1 sterilization back
Can find out by table 1; Chemical examination appliance of the present invention is used sterilized solution, and the sterilization rate of sterilization rate and high-pressure sterilizing pot is suitable, can reach 100%; But need not HTHP; Both having reduced the input of equipment, and reduced the danger coefficient in the operating process again, is relatively to have a kind of chemical examination appliance of application value to use sterilized solution in the check field.
Claims (2)
1. a chemical examination appliance is used sterilized solution, it is characterized in that being processed by the component of following weight portion:
Dimethyl sulfoxide (DMSO) 40g, polyhexamethylene guanide 50g, ethanol 200g, dodecyl sodium sulfate 5g, tarragon 20g, folium isatidis 30g, Chinese ephedra 15g, madder 10g, rhizoma alismatis 30g;
The preparation method is following:
Tarragon, folium isatidis, Chinese ephedra, madder, rhizoma alismatis are added in the boiling water of 10 times of weight and decoct half an hour, filter, filter residue adds in the boiling water of 3 times of raw material weights again and decocted 1 hour; Filter; Merge filtrating twice, being concentrated into relative density is 1.2, adds dimethyl sulfoxide (DMSO), polyhexamethylene guanide, ethanol and dodecyl sodium sulfate; Stir, promptly get.
2. chemical examination appliance according to claim 1 is used sterilized solution, it is characterized in that addition is 80-100g/kg with using in the water of chemical examination appliance with 20-30 ℃ of sterilized solution adding, and sterilization time is 1-2 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210000612 CN102524317B (en) | 2012-01-04 | 2012-01-04 | Sterilized solution for assay detection instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210000612 CN102524317B (en) | 2012-01-04 | 2012-01-04 | Sterilized solution for assay detection instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102524317A true CN102524317A (en) | 2012-07-04 |
| CN102524317B CN102524317B (en) | 2013-08-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210000612 Expired - Fee Related CN102524317B (en) | 2012-01-04 | 2012-01-04 | Sterilized solution for assay detection instrument |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54154507A (en) * | 1978-05-22 | 1979-12-05 | Eisai Co Ltd | Inactivating agents for hbs anitgen |
| CN1188009A (en) * | 1997-01-14 | 1998-07-22 | 赵清涛 | Chinese medicine cold-preventing sterilizing fragrant fog and its making method |
| CN101028010A (en) * | 2006-02-27 | 2007-09-05 | 权力敏 | Chinese-herb disinfectant |
| CN101156608A (en) * | 2007-11-23 | 2008-04-09 | 姜伟 | Natural disinfection deodorization fumigant for poultry house |
| CN101310726A (en) * | 2007-05-22 | 2008-11-26 | 上海利康消毒高科技有限公司 | Alcohol-containing polyhexamethylguanidine iodine disinfectant and preparation method thereof |
| KR20090064771A (en) * | 2007-12-17 | 2009-06-22 | (주)제로투세븐 | The water containing tissue having the excellent effect of prophylaxis and treatment against skin ailments |
| WO2011135577A1 (en) * | 2010-04-29 | 2011-11-03 | Bios Agricorp Pvt. Ltd. | Polyhexamethyleneguanidine and its salts based disinfectant. |
-
2012
- 2012-01-04 CN CN 201210000612 patent/CN102524317B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54154507A (en) * | 1978-05-22 | 1979-12-05 | Eisai Co Ltd | Inactivating agents for hbs anitgen |
| CN1188009A (en) * | 1997-01-14 | 1998-07-22 | 赵清涛 | Chinese medicine cold-preventing sterilizing fragrant fog and its making method |
| CN101028010A (en) * | 2006-02-27 | 2007-09-05 | 权力敏 | Chinese-herb disinfectant |
| CN101310726A (en) * | 2007-05-22 | 2008-11-26 | 上海利康消毒高科技有限公司 | Alcohol-containing polyhexamethylguanidine iodine disinfectant and preparation method thereof |
| CN101156608A (en) * | 2007-11-23 | 2008-04-09 | 姜伟 | Natural disinfection deodorization fumigant for poultry house |
| KR20090064771A (en) * | 2007-12-17 | 2009-06-22 | (주)제로투세븐 | The water containing tissue having the excellent effect of prophylaxis and treatment against skin ailments |
| WO2011135577A1 (en) * | 2010-04-29 | 2011-11-03 | Bios Agricorp Pvt. Ltd. | Polyhexamethyleneguanidine and its salts based disinfectant. |
Non-Patent Citations (2)
| Title |
|---|
| 薛广波: "《现代消毒学》", 31 December 2002, 人民军医出版社 * |
| 薛广波: "消毒灭菌方法研究进展", 《中国感染控制杂志》 * |
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| CN102524317B (en) | 2013-08-28 |
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