CN102524077A - Methods for quickly rooting and exercising tissue culture seedlings of lowbush blueberries - Google Patents
Methods for quickly rooting and exercising tissue culture seedlings of lowbush blueberries Download PDFInfo
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- CN102524077A CN102524077A CN2012100486203A CN201210048620A CN102524077A CN 102524077 A CN102524077 A CN 102524077A CN 2012100486203 A CN2012100486203 A CN 2012100486203A CN 201210048620 A CN201210048620 A CN 201210048620A CN 102524077 A CN102524077 A CN 102524077A
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Abstract
The invention discloses methods for quickly rooting and exercising tissue culture seedlings of lowbush blueberries. The methods comprise the following steps of: inoculating stems of the tissue culture seedlings of the lowbush blueberries onto a WPM (woody plant medium) basic culture medium and adding 10mu M IBA (indolebutyric acid) to the rooting culture medium for rooting culture and spraying 108 Shenggenbao with concentration being 0.14-0.16% once every seven days at the seedling exercising stage. Compared with the conventional methods for rooting and exercising the tissue culture seedlings, the methods have the following beneficial effects that: the rooting rates of the annual seedlings of the lowbush blueberries can be improved to be above 90%; the survival rates of the new seedlings are improved to be above 90% from 50%; and meanwhile, the quality of the new seedlings is obviously improved.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to short clump blueberry tissue cultivating seedling and take root fast and hardening off method.
Background technology
Blueberry formal name used at school cowberry belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium spp.) plant.The blueberry taste of fruit is unique, and is nutritious, is described as the king of berry.The anthocyan that contains high-load in its fruit has and delays senility, prevents myopia, opposing heart disease, anticancer, antiulcer, effect such as anti-oxidant and anti-interference.
The artificial cultivation of blueberry is T&B so far, but because of its development rapidly, supply falls short of demand for the blueberry nursery stock, become the technical bottleneck of the national blueberry industry development of restriction.Short clump blueberry is that Agricultural Research Institute introduces from Daxing'an Mountainrange by the wild species in original producton location or its procreation kind of cultivation.Main feature is that drought-resistant ability is stronger, and has very strong cold tolerance (minimum-40 ℃), on barren ground, hilly ground that rock is exposed, level land etc. all can normal growths.Cultivation management technology is required simply, and the utmost point is suitable for northeast high and cold mountain area large tracts of land commercialization cultivation.The seminal propagation cycle of short clump blueberry routine is long, is difficult to satisfy the needs of large-scale production and mass marketed.
But tissue culture technique has the envirment factor Artificial Control; Breeding cycle is short, speed is fast, but produces in the anniversary, saves and produces material; Also have advantages such as the original merit of the plant of maintenance simultaneously; Bred so extensively be used in the batch production of a lot of plant seedlings, tissue culture obtains in the short time that in the improved speed of accelerating the blueberry cultivation condition application is also arranged in a large amount of tissue cultivating seedling in recent years; But blueberry tissue cultivating seedling plant is slim and frahile, rooting rate is lower, if directly carry out cuttage or transplant that then survival rate is very low.
Summary of the invention
The object of the present invention is to provide a kind of short clump blueberry tissue cultivating seedling to take root fast and hardening off method, for blueberry provide a kind of simple to operate, save time, the fast breeding method of with low cost, stability and high efficiency.
The method that blueberry tissue cultivating seedling provided by the invention is taken root fast is characterized in that, comprises the steps:
The blueberry young sprout that enrichment culture is obtained is seeded on the root media, and this medium is: the WPM minimal medium adds 10 μ M IBA (indolebutyric acid), 25g/L sucrose and 6g/L agar, transfers pH to 5.2～5.4, autoclave sterilization 15min.After around cultivating, root refines seedling when being about to 1cm.
The hardening off method of the seedling of taking root that said method is turned out is characterized in that, the transplantation of seedlings of taking root of turning out is carried out Routine Management in refining seedling matrix, and the constituent of this refining seedling matrix is the Northeast's ordinary chernozem and river sand, and ratio is (1～2): 1 (volume ratio).
Above-mentioned hardening off method is characterized in that: every in refining seedling process sprayed concentration at a distance from 7 days be 108 of the 0.14%-0.16% treasured (exogenous auxin of synthetic) of taking root, and can obviously improve the survival rate of transplanted seedling.
The acquisition step of above-mentioned blueberry tissue cultivating seedling is following: select eugonic shoot, sterilize after cutting off blade, it is that the segment of 1～2 bud is subsequent use that the blueberry stem section that disinfects is cut.
The acquisition of above-mentioned blueberry tissue cultivating seedling also comprises enrichment culture, and said enrichment culture comprised the steps: the stem section that a last step obtains is seeded on the proliferated culture medium, and this medium is: the WPM minimal medium adds 15mg/L2-ip (N
6Isopentenyl gland purine), 25g/L sucrose and 6g/L agar, transfer pH to 5.2, autoclave sterilization 15min.After around cultivating, tissue cultivating seedling grows a large amount of young sprouts.
The present invention has adopted two kinds of special matrix, i.e. root media and refining seedling matrix.That root media adds is 10 μ M IBA (indolebutyric acid); Refining seedling matrix is (1～2): the black earth of 1 (volume ratio) and river sand combination.
It is 0.14%～0.16% 108 treasured of taking root that the present invention has adopted refining seedling stage spraying concentration, makes the survival rate of transplanted seedling can reach root average energy growth 0.9cm after 99%, month.
The invention has the beneficial effects as follows: 1. overcome the difficult problem of taking root in the short clump blueberry bottle, and rooting rate is brought up to more than 90%; 2. the survival rate of seedling reaches 99%.
It is low to the invention solves take root difficulty, reproduction coefficient of short clump blueberry tradition cottage method, and the difficult problem that the tissue cultivating seedling plant is slim and frahile, rooting rate is lower provides a kind of method of suitability for industrialized production high-quality finished product seedling for the development and use of short clump blueberry.
Description of drawings
The short clump blueberry stem section of growing on Fig. 1 proliferated culture medium
The root of the short clump blueberry stem section of growing on Fig. 2 root media
Fig. 3 refines the short clump blueberry transplanted seedling in the seedling matrix
Embodiment
Below in conjunction with specific embodiment the present invention is described further.
Embodiment 1
Short clump blueberry is drawn materials and is sterilized
Select eugonic shoot, cut off blade, the stem section is rinsed well with running water and is placed on 30s in 75% alcohol; Remove alcohol, use rinsed with sterile water; Change the 3～8min that sterilizes in 0.1% mercuric chloride over to, use rinsed with sterile water then 3～6 times, and in sterile water, soak 20min; Take out at last that the stem section is cut is that the segment of 1～2 bud is subsequent use.
Short clump blueberry enrichment culture
On superclean bench; Resulting stem section in the first step is transferred on the proliferated culture medium; This medium is: the WPM minimal medium adds 15mg/L2-ip (N6 isopentenyl gland purine), 25g/L sucrose and 6g/L agar, transfers pH to 5.2, autoclave sterilization 15min.After around cultivating, tissue cultivating seedling grows a large amount of young sprouts, gets into next step behind the subculture 3～4 times of using the same method.
Short clump blueberry culture of rootage
The stem section that a last step is obtained is seeded on the root media, and this medium is: the WPM minimal medium adds 10 μ M IBA (indolebutyric acid), 25g/L sucrose and 6g/L agar, transfers pH to 5.2, autoclave sterilization 15min.About around cultivating, when reaching 1cm, refines root seedling.
Short clump blueberry acclimatization and transplants
The seedling of taking root that the 3rd step obtained was opened bottle cap in advance in three days.During transplanting, carefully take out seedling, the medium of root is cleaned with tweezers; Be planted in black earth and river sand ratio and be (1～2): in the matrix of 1 (volume ratio); In group training chamber, relative moisture is controlled between 60%～90% in the canopy in the plastic greenhouse of complete closed, and temperature is controlled at 25 ℃.Every at a distance from 7 days spraying concentrations be 0.14% 108 take root treasured once, the survival rate of transplanted seedling can reach after 99%, one month the root average energy 0.9cm that grows.
Embodiment 2
Short clump blueberry is drawn materials and is sterilized
Select eugonic shoot, cut off blade, the stem section is rinsed well with running water and is placed on 30s in 75% alcohol; Remove alcohol, use rinsed with sterile water; Change the 3～8min that sterilizes in 0.1% mercuric chloride over to, use rinsed with sterile water then 3～6 times, and in sterile water, soak 20min; Take out at last that the stem section is cut is that the segment of 1～2 bud is subsequent use.
Short clump blueberry enrichment culture
On superclean bench; Resulting stem section in the first step is transferred on the proliferated culture medium; This medium is: the WPM minimal medium adds 15mg/L2-ip (N6 isopentenyl gland purine), 25g/L sucrose and 6g/L agar, transfers pH to 5.2, autoclave sterilization 15min.After around cultivating, tissue cultivating seedling grows a large amount of young sprouts, gets into next step behind the subculture 3～4 times of using the same method.
Short clump blueberry culture of rootage
The stem section that a last step is obtained is seeded on the root media, and this medium is: the WPM minimal medium adds 10 μ M IBA (indolebutyric acid), 25g/L sucrose and 6g/L agar, transfers pH to 5.4, autoclave sterilization 15min.About around cultivating, when reaching 1cm, refines root seedling.
Short clump blueberry acclimatization and transplants
The seedling of taking root that the 3rd step obtained was opened bottle cap in advance in three days.During transplanting, carefully take out seedling, the medium of root is cleaned with tweezers; Be planted in black earth and river sand ratio and be (1～2): in the matrix of 1 (volume ratio); In group training chamber, relative moisture is controlled between 60%～90% in the canopy in the plastic greenhouse of complete closed, and temperature is controlled at 25 ℃.Every sprayed at a distance from 7 days 0.16% 108 take root treasured once, the survival rate of transplanted seedling can reach after 99%, one month the root average energy 0.9cm that grows.
Claims (5)
1. one kind short method that clump blueberry tissue cultivating seedling is taken root fast; It is characterized in that said method is following: the blueberry young sprout that enrichment culture is obtained is seeded on the root media; This medium is: the WPM minimal medium adds 10 μ M IBA (indolebutyric acid), 25g/L sucrose and 6g/L agar; Transfer pH to 5.2～5.4, autoclave sterilization 15min.After around cultivating, root refines seedling when being about to 1cm.
2. the hardening off method of the seedling of taking root of turning out according to claim 1 cultural method; It is characterized in that the transplantation of seedlings of taking root that claim 1 is turned out is carried out Routine Management in refining seedling matrix; The constituent of this refining seedling matrix is the Northeast's ordinary chernozem and river sand, and ratio is (1～2): 1 (volume ratio).
3. hardening off method according to claim 2 is characterized in that: in refining seedling process, add concentration and be 0.14%～0.16% 108 treasured of taking root and can obviously improve the survival rate of transplanted seedling.
4. according to arbitrary described method among the claim 1-3, it is characterized in that: it is following that the tissue cultivating seedling of said blueberry obtains step: select eugonic shoot, sterilize after cutting off blade, it is that the segment of 1～2 bud is subsequent use that the blueberry stem section that disinfects is cut.
5. according to the method described in the claim 4; It is characterized in that: the tissue cultivating seedling of said blueberry obtains step and also comprises enrichment culture; Said enrichment culture comprises that step is following: will go up the stem section that obtains of step and be seeded on the proliferated culture medium, this medium is: the WPM minimal medium adds 15mg/L2-ip (N
6Isopentenyl gland purine), 25g/L sucrose and 6g/L agar, transfer pH to 5.2, autoclave sterilization 15min.After around cultivating, tissue cultivating seedling grows a large amount of young sprouts.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926456A (en) * | 2017-12-15 | 2018-04-20 | 阜阳恒明悦农业科技有限公司 | A kind of high blueberry cultivation method of survival rate |
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699991A (en) * | 2009-10-23 | 2010-05-05 | 鲁东大学 | Method for inducing blueberries to root by using liquid culturing media and permeable matrix |
| CN101869078A (en) * | 2010-07-14 | 2010-10-27 | 长春百瑞蓝莓科技发展有限公司 | Seed-cultivating method of blueberry by means of tissue cultivation and micropropagation |
-
2012
- 2012-02-29 CN CN2012100486203A patent/CN102524077A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101699991A (en) * | 2009-10-23 | 2010-05-05 | 鲁东大学 | Method for inducing blueberries to root by using liquid culturing media and permeable matrix |
| CN101869078A (en) * | 2010-07-14 | 2010-10-27 | 长春百瑞蓝莓科技发展有限公司 | Seed-cultivating method of blueberry by means of tissue cultivation and micropropagation |
Non-Patent Citations (3)
| Title |
|---|
| ZHAO GUANG-JIE ET AL: "In vitro propagation and Ex vitro Rooting of Blueberry Plantlets", 《PLANT TISSUE CULT. & BIOTECH》 * |
| 董朝莉: "蓝莓芽诱导与再生研究", 《广西农业科学》 * |
| 韦庆和等: "108生根宝在林业育苗中的应用试验", 《防护林科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926456A (en) * | 2017-12-15 | 2018-04-20 | 阜阳恒明悦农业科技有限公司 | A kind of high blueberry cultivation method of survival rate |
| CN108668659A (en) * | 2018-07-18 | 2018-10-19 | 苏州枫彩生态科技集团有限公司 | A kind of method of the outer cuttage root-taking of blueberry bottle |
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Application publication date: 20120704 |