CN102507956A - Detachable protein film chip - Google Patents
Detachable protein film chip Download PDFInfo
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- CN102507956A CN102507956A CN2011103214600A CN201110321460A CN102507956A CN 102507956 A CN102507956 A CN 102507956A CN 2011103214600 A CN2011103214600 A CN 2011103214600A CN 201110321460 A CN201110321460 A CN 201110321460A CN 102507956 A CN102507956 A CN 102507956A
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- loam cake
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Abstract
The invention relates to a detachable protein film chip. The detachable protein film chip comprises a pedestal and a reaction bar, wherein the pedestal comprises a bottom plate and a group of fixed seats; the reaction bar comprises a base, a gasket, a film chip and an upper cover, wherein the base compries a base main body which are provided with a row of base holes running from top to bottom from left to right; a row of gasket holes are arranged on the gasket; the gasket is pasted and fixed on the base main body of the base from the lower surface of the gasket; the film chip is pasted and fixed on the upper surface of the gasket from the lower surface of the film chip; the upper cover is fixed on the base from the upper part of the upper cover; the cutting edge of the upper cover penetrates through the film chip, and is inserted into the gasket, so that the film chip is cut into protein miogrorray areas which are not connected, and the positions of the protein miogrorray areas correspond to the position of each base hole and each upper cover hole; and the reaction bar is clamped and sheathed on the fixed seat corresponding to the pedestal through the base hole on the base. The detachable protein film chip provided by the invention is concise in structure, is convenient in assembling and can be used for detecting small number of people.
Description
Technical field
The present invention relates to a kind of biochip, especially a kind of protein-chip.
Background technology
The albuminous membranae chip is that protein is fixed on the film medium carrier in an orderly manner, and making it becomes the biochip that detects usefulness, then it is carried out the immune detection analysis.The albuminous membranae chip can use the chemiluminescence enzyme immunoassay method to carry out check and analysis; Its principle for mark the antibody of horseradish peroxidase (antigen) and chip effect, the antibody (antigen) that is complementary with protein on the chip after immune response takes place in antigen corresponding with it (antibody), formation solid-phase coating compound; After washing; Add luminous agent, the enzymatic luminous agent is luminous, goes out to measure the concentration of thing subsequently through collection light signal computable analysis.
Chinese patent document CN2497313Y (application number is 01253423.4) has announced a kind of protein chip; Comprise lid and box body; Lid is one the plate of porous to be arranged, and box body is formed by being pasted on porose pad and the porose plate that is pasted on the pad below that the pad top has film, a biadhesive of a plurality of albumen dot matrix.This protein chip can be applicable to the parallel detection of many indexs.Though the demarcation strip in each hole of this protein chip protrudes in this plastic plate above and below; And be close on the diaphragm; But because diaphragm joins together and has permeability, each hole can be that seepage takes place media each other with the diaphragm when adding reagent, causes the cross pollution between the sample.In addition, the detection hole number of monolithic protein chip is fixed, and must use whole protein chip when detecting at every turn, otherwise idle detection hole can receive pollution so that scrap.So the requirement when this chip can't satisfy emergency treatment (sample size is less, require detection time short) is only applicable to bigger large hospital of specimen amount or blood station, is not suitable for the use of small hospital and R&D institution.
Chinese patent document CN102023205A (application number is 200910195412.4) has announced that a kind of dismantled and assembled film chip comprises assemblnig film chip reaction bar and pedestal.Chinese patent document CN201488997U (application number is 200920210323.8) has announced that the film chip reaction bar of above-mentioned dismantled and assembled film chip comprises base and chamber; Chamber engages with upper end reaction chamber pedestal through inwall each other, combines with base to constitute the reaction bar.The side leakage of above-mentioned dismantled and assembled film chip ability blocking reaction liquid is avoided cross pollution between the adjacent sample, and can be taken according to actual sample quantity, needn't use entire chip.But because the film of band biomolecule dot matrix is more crisp, the film chip of this dismantled and assembled film chip reacts the structure of bar, and is unfavorable for the assembling of pad and film, and film very easily damages.
Summary of the invention
The purpose of this invention is to provide a kind of simple for structurely, easy to assembly, can carry out the removable albuminous membranae chip that person of low position's part is detected.
A kind of technical scheme of realization the object of the invention provides a kind of removable albuminous membranae chip and comprises base and reaction bar, and base comprises base plate and one group of holder; The central authorities of holder all offer the holder hole of up/down perforation; The rectangular array distribution of holder is connected on the base plate from the top by its bottom,
The reaction bar comprises pedestal, pad, diaphragm and loam cake; Pedestal comprises Base body; Base body is a strip, along about to setting; Offer the pedestal hole of row's up/down perforation on the Base body from left to right, the shape in pedestal hole and the shape of holder are complementary; About the quantity in pedestal hole equals on the base to the quantity of holder;
Pad is the foam pad that upper surface and lower surface all have viscosity; Offer row's pad hole on the pad; The quantity in pad hole is identical with size with the quantity in pedestal hole with size; Pad is pasted and fixed on by its lower surface on the Base body of pedestal, and the position in each pad hole is all corresponding with the position in each pedestal hole; The shape of diaphragm is identical with the shape of pad, and diaphragm is pasted and fixed on by its lower surface on the upper surface of pad, on the upper surface of diaphragm with corresponding position, pedestal hole be protein microarray zone;
Loam cake comprises loam cake main body and cutting edge; To setting, offer the loam cake hole of row's up/down perforation on the loam cake main body from left to right about loam cake main body edge, the position between the adjacent loam cake hole is a dividing plate; The quantity in loam cake hole is identical with size with the quantity in pedestal hole with size, and the position in loam cake hole is corresponding with the pedestal hole; Cutting edge is provided with along each dividing plate of loam cake main body, and is connected the bottom of dividing plate by its top; The height of cutting edge is less than or equal to the thickness of pad,
Loam cake from upper fixed on pedestal; The cutting edge of loam cake passes diaphragm, inserts in the pad, and the position that diaphragm is cut into mutual disjunct protein microarray region protein matter microarray zone is all corresponding with the position in each pedestal hole and each loam cake hole;
The reaction bar is fastened on the corresponding holder of base by the pedestal hole on its pedestal.
The pedestal that a kind of optimal technical scheme of the present invention is above-mentioned reaction bar also comprises 2 clamping heads; 1 clamping head is connected on the left surface of Base body, and 1 clamping head is connected on the right flank of Base body in addition; The loam cake of above-mentioned reaction bar also comprises 2 Access Divisions; 2 Access Divisions all are connected on the loam cake main body from the below, and lay respectively at the below, left side and the below, right side of loam cake main body; Offer the card that the clamping head with pedestal is complementary on the Access Division; 2 clamping heads of pedestal are stuck in the card of the Access Division of corresponding loam cake separately.
The pedestal that another kind of optimal technical scheme of the present invention is above-mentioned reaction bar also comprises 6 clamping heads; The clamping head all is connected on the Base body, and lays respectively at left surface, right flank, the left part of leading flank, the right part of leading flank, the left part of trailing flank and the right part of trailing flank of Base body; The loam cake of above-mentioned reaction bar also comprises the Access Division; The Access Division is a rectangle frame, and the Access Division is connected on the loam cake main body from the below, and is positioned at the edge of loam cake main body; Offer 6 card that the clamping head with pedestal is complementary on the Access Division; 6 clamping heads of pedestal are stuck in the card of the Access Division of corresponding loam cake separately.
One group of holder of above-mentioned base forms 2 to 12 horizontally-arrangeds, 2 to 16 vertical rows; The quantity of above-mentioned reaction bar is less than or equal to the horizontally-arranged number of holder.
Each holder of above-mentioned base is square column, and the leading flank of each holder is equipped with projection; Offer the groove that is complementary with projection holder that Open Side Down on the front side board of the Base body of the pedestal of above-mentioned reaction bar; The projection of each holder all is positioned at corresponding grooves.
The top of the Base body of the pedestal of above-mentioned reaction bar offers the pad groove that area is slightly less than the Base body upper surface; The pedestal hole all is positioned at the pad groove; The shape of above-mentioned pad is identical with the shape of pad groove; And pad is positioned at the pad groove.
Above-mentioned base also comprises peripheral block; The periphery block is connected the edge of base plate from the top by its bottom; The intersection in the gap between the gap between each horizontally-arranged of the holder on the base plate and each vertical row all offers the circular hole of up/down perforation; The periphery block comprises front side block, rear side block, left side block and right side block; The left side is block to be equipped with protruding left side block lug boss to the right with the corresponding position of holder each horizontally-arranged; The right side block and the corresponding position of holder each horizontally-arranged are equipped with protruding right side block lug boss left; The shape of right side block lug boss is identical with the shape of left side block lug boss;
The pedestal of above-mentioned reaction bar also comprises left positioner portion and right positioner portion; Left positioner portion is connected on the Base body from left, and is positioned at the below of the clamping head in left side; Left positioner portion is provided with the left positioner groove that the left side block lug boss of opening shape and base left is complementary; Right positioner portion is connected on the Base body from left, and is positioned at the below of the clamping head in left side; Right positioner portion is provided with the right positioner groove that the right side block lug boss of opening shape and base to the right is complementary;
The left side block boss of the left side block of base is in the left positioner groove of the left positioner portion of pedestal, and the right side block boss of the right side block of base is in the right positioner groove of the right positioner portion of pedestal;
The top of rear side block is printed on the corresponding mark in position with each vertical holder of arranging from left to right successively; The top of left side block is printed on the corresponding mark in position with the holder of each horizontally-arranged; The right front at the top of front side block is provided with breach.
Above-mentioned base also comprises side plate; Side plate has 2, is divided into left plate and right plate; Left plate is connected on the base plate from the top by its lower end, and is positioned at the left side central portion of base plate; The left side central portion of left plate is provided with protruding left plate Access Division left; Right plate is connected on the base plate from the top by its lower end, and is positioned at the right side central of base plate; The right side central of right plate is provided with protruding right plate Access Division to the right; The left side block is divided into left side block leading portion that is positioned at left plate the place ahead and the left side block back segment that is positioned at the left plate rear; The right side block is divided into right side block leading portion that is positioned at right plate the place ahead and the right side block back segment that is positioned at the right plate rear.
The height of the cutting edge of the loam cake of above-mentioned reaction bar is 0.5 to 5mm; The bottom of the xsect of cutting edge be the downward isosceles triangle of drift angle or minor face under isosceles trapezoid.
Above-mentioned diaphragm is a nitrocellulose membrane, and each the protein microarray zone on the diaphragm comprises 2 to 1000 protein spots sampling points.
The present invention has positive effect:
(1) the reaction bar of removable albuminous membranae chip of the present invention is simple for structure; When assembling, each protein microarray zone of diaphragm is cut open through cutting edge; Be convenient to assembling; Effectively raise this production efficiency of products and yield rate, fundamentally avoided the cross pollution between the sample.This chip is made up of base and reaction bar, and the user can use the reaction bar of respective numbers according to actual sample quantity, has improved the utilization factor of product greatly, has avoided waste, and because base can be reused, has also practiced thrift corresponding cost.This chip can be realized many indexs, little person-portion, parallelization disease detection.
(2) below in each protein microarray of removable albuminous membranae chip of the present invention zone corresponding the holder hole on the holder of pedestal hole and chip pad of pedestal of reaction bar, this below hollow out be designed with the cleaning that is beneficial to chip.
(3) loam cake of the reaction bar of removable albuminous membranae chip of the present invention and pedestal are fixed together through clip assembly is removable, and be simple for structure, easy to assembly.
(4) holder on the base of removable albuminous membranae chip of the present invention is provided with projection, and the pedestal of reaction bar is provided with corresponding grooves with it, and direction can be put upside down when having avoided the reaction bar to be fixed on the base.
Description of drawings
Fig. 1 is the perspective view of the removable albuminous membranae chip in the embodiment of the invention 1 when observing from the top;
Perspective view when observing from the upper right side when Fig. 2 has only 1 reaction bar for the removable albuminous membranae chip among Fig. 1;
Fig. 3 is the perspective view of the reaction bar among Fig. 2;
Fig. 4 is the perspective exploded view of the reaction bar among Fig. 3;
Fig. 5 is the loam cake of the reaction bar among Fig. 4 structural representation during from beneath;
Fig. 6 is the cut-open view of the B-B face after the local amplification in the A place among Fig. 5;
Fig. 7 is the perspective view of the removable albuminous membranae chip in the embodiment of the invention 2 when observing from the top;
Perspective view when observing from the upper right side when Fig. 8 has only 1 reaction bar for the removable albuminous membranae chip among Fig. 7;
Fig. 9 is the perspective view of the reaction bar among Fig. 8;
Figure 10 is the perspective exploded view of the reaction bar among Fig. 9;
Figure 11 is the loam cake of the reaction bar among Figure 10 structural representation during from beneath;
Figure 12 is the cut-open view of the D-D face after the local amplification in the C place among Figure 11.
The Reference numeral of above-mentioned accompanying drawing is following:
Base 1,
Periphery block 12, front side block 12-1, breach 12-1-1, rear side block 12-2; Left side block 12-3, left side block leading portion 12-3-1, left side block back segment 12-3-2; Left side block lug boss 12-3-3, right side block 12-4, right side block leading portion 12-4-1; Right side block back segment 12-4-2, right side block lug boss 12-4-3
Holder 13, holder hole 13-1, projection 13-2,
Side plate 14, left plate 14-1, left plate Access Division 14-1-1, right plate 14-2, right plate Access Division 14-2-1,
Pedestal 21, Base body 21-1, pad groove 21-1-1, pedestal hole 21-1-2, groove 21-1-3, the 21-2 of left positioner portion, left positioner groove 21-2-1, the 21-3 of right positioner portion, right positioner groove 21-3-1, a clamping 21-4,
Diaphragm 23, protein microarray zone 23-1, protein spots sampling point 23-1-1,
Embodiment
In the embodiment of the present invention, be according to carrying out under the 1 horizontal positioned state of the base shown in Fig. 1 to the orientation of each parts and the description of mutual relationship.
(embodiment 1)
See Fig. 1 to Fig. 3, the removable albuminous membranae chip of present embodiment comprises base 1 and 6 reaction bars 2.
See Fig. 3, base 1 is the integrated through injection molding part, comprises base plate 11, peripheral block 12, holder 13 and side plate 14.
Side plate 14 has 2, is divided into left plate 14-1 and right plate 14-2.Left plate 14-1 is connected on the base plate 11 from the top by its lower end, and is positioned at the left side central portion of base plate 11.The left side central portion of left plate 14-1 is provided with protruding left plate Access Division 14-1-1 left.Right plate 14-2 is connected on the base plate 11 from the top by its lower end, and is positioned at the right side central of base plate 11.The right side central of right plate 14-2 is provided with protruding right plate Access Division 14-2-1 to the right.
Periphery block 12 comprises front side block 12-1, rear side block 12-2, left side block 12-3 and right side block 12-4.The top of rear side block 12-2 is printed on " A, B, C, D, E, F, G, H " 8 English alphabets from left to right successively, and the position of each English alphabet is corresponding with the position of 8 holders 13 of each horizontally-arranged.The right front at the top of front side block 12-1 is provided with breach 12-1-1.Left side block 12-3 is divided into left side block leading portion 12-3-1 that is positioned at left plate 14-1 the place ahead and the left side block back segment 12-3-2 that is positioned at left plate 14-1 rear.Left side block back segment 12-3-2 is equipped with protruding left side block lug boss 12-3-3 to the right with first row, second row's holder, 13 corresponding positions.Left side block leading portion 12-3-2 is equipped with protruding left side block lug boss 12-3-3 to the right with the 5th row, the 6th row's holder 13 corresponding positions.Left side block lug boss 12-3-3 is semicircle, and the top of left side block 12-3 is printed on the corresponding arabic numeral of row's number with holder 13.Right side block 12-4 is divided into right side block leading portion 12-4-1 that is positioned at right plate 14-2 the place ahead and the right side block back segment 12-4-2 that is positioned at right plate 14-2 rear.Right side block back segment 12-4-2 is equipped with protruding right side block lug boss 12-4-3 left with first row, second row's holder, 13 corresponding positions.Right side block leading portion 12-4-2 is equipped with protruding right side block lug boss 12-4-3 left with the 5th row, the 6th row's holder 13 corresponding positions.The shape of right side block lug boss 12-4-3 is identical with the shape of left side block lug boss 12-3-3.
See Fig. 4 and Fig. 5, reaction bar 2 comprises pedestal 21, pad 22, diaphragm 23 and loam cake 24.
Pedestal 21 is the integrated through injection molding part, comprises Base body 21-1, the 21-2 of left positioner portion, the 21-3 of right positioner portion and a clamping 21-4.Base body 21-1 is shaped as strip, along about to being horizontally disposed with.The top of Base body 21-1 offers the pad groove 21-1-1 that area is slightly less than the upper surface of Base body 21-1.Offer the pedestal hole 21-1-2 of 8 up/down perforations on the Base body 21-1 from left to right, 8 pedestal hole 21-1-2 all are positioned at pad groove 21-1-1, and the shape of pedestal hole 21-1-2 and the shape of holder 13 are complementary.Offer the groove 21-1-3 that is complementary with projection 13-2 holder 13 that Open Side Down on the front side board of Base body 21-1.A clamping 21-4 has 2, and 1 clamping 21-4 wherein is arranged on the left surface of Base body 21-1, and 1 clamping 21-4 is arranged on the right flank of Base body 21-1 in addition.The 21-2 of left positioner portion is connected on the Base body 21-1 from left, and is positioned at the below of a clamping 21-4 in left side.The 21-2 of left positioner portion is provided with the left positioner groove 21-2-1 that the left side block lug boss 12-3-3 of opening shape and base 1 left is complementary.The 21-3 of right positioner portion is connected on the Base body 21-1 from left, and is positioned at the below of a clamping 21-4 in left side.The 21-3 of right positioner portion is provided with the right positioner groove 21-3-1 that the right side block lug boss 12-4-3 of opening shape and base 1 to the right is complementary.
Diaphragm 23 is a nitrocellulose membrane, and this nitrocellulose membrane is that the preparation method through the disclosed a kind of protein-chip of Chinese patent document CN100341570C (patent No. is ZL03116076.X) makes.The shape of diaphragm 3 is identical with the shape of pad 22.Diaphragm 23 is pasted and fixed on from the top on the upper surface of pad 22 by its lower surface, on the upper surface of diaphragm 23 with the corresponding position of 21-1-2, pedestal hole be protein microarray zone 23-1.Each protein microarray zone 23-1 comprises 10 protein spots sampling point 23-1-1.
See Fig. 5 to Fig. 7, loam cake 24 is the integrated through injection molding part, comprises loam cake main body 24-1, Access Division 24-2 and cutting edge 24-3.To being horizontally disposed with, offer the loam cake hole 24-1-1 of 8 up/down perforations on the loam cake main body 24-1 from left to right about loam cake main body 24-1 edge, the position between the adjacent loam cake hole 24-1-1 is dividing plate 24-1-2.The size of loam cake hole 24-1-1 is big or small identical with pedestal hole 21-1-2's, and the position of loam cake hole 24-1-1 is corresponding with pedestal hole 21-1-2.Access Division 24-2 has 2, all is connected on the loam cake main body 24-1 from the below, and lays respectively at below, left side and the below, right side of loam cake main body 24-1.Offer the card 24-2-1 that the clamping 21-4 with pedestal 21 is complementary on the 24-2 of Access Division.Cutting edge 24-3 is provided with along each dividing plate 24-1-2 of loam cake main body 24-1, and is connected the bottom of dividing plate 24-1-2 by its top.The height of cutting edge 24-3 is 1.8mm, and the top of the xsect of cutting edge 24-3 is rectangle, and the bottom of its xsect is the isosceles right triangle (seeing Fig. 6 and Fig. 7 for details) of down at right angles.
See Fig. 5,2 clamping 21-4 of pedestal 21 are stuck among the card 24-2-1 of Access Division 24-2 of corresponding separately loam cake 24, thus make loam cake 24 from upper fixed on pedestal 21.The cutting edge 24-3 of loam cake 24 passes diaphragm 23; Insert in the pad 22; Thereby each protein microarray zone 23-1 of diaphragm 23 is cut open; Form 8 mutual disjunct protein microarray zone 23-1, and the position of each piece protein microarray zone 23-1 is all corresponding with the position of each pedestal hole 21-1-2 and each loam cake hole 24-1-1.
See Fig. 2; Reaction bar 2 is fastened on the corresponding holder 13 of base 1 by the pedestal hole 21-1-2 on its pedestal 21; The projection 13-2 of holder 13 is positioned at the corresponding grooves 21-1-3 of Base body 21-1; The left side block lug boss 12-3-3 of the left side block 12-3 of base 1 is positioned at the left positioner groove 21-2-1 of the 21-2 of left positioner portion of pedestal 21; The right side block lug boss 12-4-3 of the right side block 12-4 of base 1 is positioned at the right positioner groove 21-3-1 of the 21-3 of right positioner portion of pedestal 21, thus make reaction bar 2 from the top the removable corresponding position that is fixed on base 1.
See Fig. 1, the removable albuminous membranae chip of present embodiment can be fixed on maximum 6 reaction bars 2 corresponding position of base 1 in use as required.
(embodiment 2)
See Fig. 7 to Figure 12, the removable albuminous membranae chip remainder of present embodiment is identical with embodiment 1, and difference is:
See Fig. 9 and Figure 10, a clamping 21-4 of the pedestal 21 of reaction bar 2 has 6.A clamping 21-4 all is connected on the Base body 21-1, and lays respectively at left surface, right flank, the left part of leading flank, the right part of leading flank, the left part of trailing flank and the right part of trailing flank of Base body 21-1.
The Access Division 24-2 of the loam cake 24 of reaction bar 2 is a rectangle frame.Access Division 24-2 is connected on the loam cake main body 24-1 from the below, and is positioned at the edge of loam cake main body 24-1.Offer 6 card 24-2-1 that the clamping 21-4 with pedestal 21 is complementary on the 24-2 of Access Division.
6 clamping 21-4 of pedestal 21 are stuck among the card 24-2-1 of Access Division 24-2 of corresponding separately loam cake 24.
See Figure 11 and Figure 12, the xsect of cutting edge 24-3 of the loam cake 24 of reaction bar 2 be minor face under the isosceles trapezoid triangle.
See Fig. 7, the removable albuminous membranae chip of present embodiment can be fixed on maximum 3 reaction bars 2 corresponding position of base 1 in use as required.
Claims (10)
1. a removable albuminous membranae chip comprises base (1) and reaction bar (2), and it is characterized in that: base (1) comprises base plate (11) and one group of holder (13); The central authorities of holder (13) all offer the holder hole (13-1) of up/down perforation; The rectangular array distribution of holder (13) is connected on the base plate (11) from the top by its bottom,
Reaction bar (2) comprises pedestal (21), pad (22), diaphragm (23) and loam cake (24); Pedestal (21) comprises Base body (21-1); Base body (21-1) is a strip, along about to setting; Offer the pedestal hole (21-1-2) of row's up/down perforation on the Base body (21-1) from left to right, the shape of the shape of pedestal hole (21-1-2) and holder (13) is complementary; About the quantity in pedestal hole (21-1-2) equals base (1) and goes up to the quantity of holder (13);
Pad (22) is the foam pad that upper surface and lower surface all have viscosity; Offer a row pad hole (22-1) on the pad (22); The quantity in pad hole (22-1) is identical with size with the quantity of pedestal hole (21-1-2) with size; Pad (22) is pasted and fixed on by its lower surface on the Base body (21-1) of pedestal (21), and the position in each pad hole (22-1) is all corresponding with the position in each pedestal hole (21-1-2); The shape of diaphragm (23) is identical with the shape of pad (22); Diaphragm (23) is pasted and fixed on by its lower surface on the upper surface of pad (22), on the upper surface of diaphragm (23) with corresponding position, pedestal hole (21-1-2) be protein microarray zone (23-1);
Loam cake (24) comprises loam cake main body (24-1) and cutting edge (24-3); To setting, offer the loam cake hole (24-1-1) of row's up/down perforation on the loam cake main body (24-1) from left to right about loam cake main body (24-1) edge, the position between the adjacent loam cake hole (24-1-1) is dividing plate (24-1-2); The quantity in loam cake hole (24-1-1) is identical with size with the quantity of pedestal hole (21-1-2) with size, and the position of loam cake hole (24-1-1) is corresponding with pedestal hole (21-1-2); Cutting edge (24-3) is provided with along each dividing plate (24-1-2) of loam cake main body (24-1), and is connected the bottom of dividing plate (24-1-2) by its top; The height of cutting edge (24-3) is less than or equal to the thickness of pad (22),
Loam cake (24) from upper fixed on pedestal (21); The cutting edge (24-3) of loam cake (24) passes diaphragm (23); Insert in the pad (22), the position that diaphragm (23) is cut into mutual disjunct protein microarray (23-1) protein microarray zone, zone (23-1) is all corresponding with the position in each pedestal hole (21-1-2) and each loam cake hole (24-1-1);
Reaction bar (2) is fastened on the corresponding holder (13) of base (1) by the pedestal hole (21-1-2) on its pedestal (21).
2. according to the described removable albuminous membranae chip of claim 1, it is characterized in that: the pedestal (21) of said reaction bar (2) also comprises 2 clamping heads (21-4); 1 clamping head (21-4) is connected on the left surface of Base body (21-1), and 1 clamping head (21-4) is connected on the right flank of Base body (21-1) in addition; The loam cake (24) of said reaction bar (2) also comprises 2 Access Divisions (24-2); 2 Access Divisions (24-2) all are connected on the loam cake main body (24-1) from the below, and lay respectively at the below, left side and the below, right side of loam cake main body (24-1); Offer the card (24-2-1) that the clamping head (21-4) with pedestal (21) is complementary on the Access Division (24-2); 2 clamping heads (21-4) of pedestal (21) are stuck in the card (24-2-1) of Access Division (24-2) of corresponding separately loam cake (24).
3. according to the described removable albuminous membranae chip of claim 1, it is characterized in that: the pedestal (21) of said reaction bar (2) also comprises 6 clamping heads (21-4); Clamping head (21-4) all is connected on the Base body (21-1), and lays respectively at left surface, right flank, the left part of leading flank, the right part of leading flank, the left part of trailing flank and the right part of trailing flank of Base body (21-1); The loam cake (24) of said reaction bar (2) also comprises Access Division (24-2); Access Division (24-2) is a rectangle frame, and Access Division (24-2) is connected on the loam cake main body (24-1) from the below, and is positioned at the edge of loam cake main body (24-1); Offer 6 card (24-2-1) that the clamping head (21-4) with pedestal (21) is complementary on the Access Division (24-2); 6 clamping heads (21-4) of pedestal (21) are stuck in the card (24-2-1) of Access Division (24-2) of corresponding separately loam cake (24).
4. according to the described removable albuminous membranae chip of one of claim 1 to 3, it is characterized in that: one group of holder (13) of said base (1) forms 2 to 12 horizontally-arrangeds, 2 to 16 vertical rows; The quantity of said reaction bar (2) is less than or equal to the horizontally-arranged number of holder (13).
5. according to the described removable albuminous membranae chip of claim 4, it is characterized in that: each holder (13) of said base (1) is square column, and the leading flank of each holder (13) is equipped with projection (13-2); Offer the groove (21-1-3) that is complementary with projections holder (13) (13-1) that Open Side Down on the front side board of the Base body (21-1) of the pedestal (21) of said reaction bar (2); The projection (13-1) of each holder (13) all is positioned at corresponding grooves (21-1-3).
6. according to the described removable albuminous membranae chip of claim 5, it is characterized in that: the top of the Base body (21-1) of the pedestal (21) of said reaction bar (2) offers the pad groove (21-1-1) that area is slightly less than Base body (21-1) upper surface; Pedestal hole (21-1-2) all is positioned at pad groove (21-1-1); The shape of said pad (22) is identical with the shape of pad groove (21-1-1); And pad (22) is positioned at pad groove (21-1-1).
7. according to the described removable albuminous membranae chip of claim 6, it is characterized in that: said base (1) also comprises peripheral block (12); Periphery block (12) is connected the edge of base plate (11) from the top by its bottom; The intersection in the gap between the gap between each horizontally-arranged of holder (13) on the base plate (11) and each vertical row all offers the circular hole (11-1) of up/down perforation; Periphery block (12) comprises front side block (12-1), rear side block (12-2), left side block (12-3) and right side block (12-4); The left side block is (12-3) to be equipped with protruding left side block lug boss (12-3-3) to the right with the corresponding position of holder each horizontally-arranged (13); The right side block is (12-4) to be equipped with protruding right side block lug boss (12-4-3) left with the corresponding position of holder each horizontally-arranged (13); The shape of right side block lug boss (12-4-3) is identical with the shape of left side block lug boss (12-3-3);
The pedestal (21) of said reaction bar (2) also comprises left positioner portion (21-2) and right positioner portion (21-3); Left positioner portion (21-2) is connected on the Base body (21-1) from left, and is positioned at the below of the clamping head (21-4) in left side; Left positioner portion (21-2) is provided with the left positioner groove (21-2-1) that left side block lug boss (12-3-3) of opening shape and base (1) left is complementary; Right positioner portion (21-3) is connected on the Base body (21-1) from left, and is positioned at the below of the clamping head (21-4) in left side; Right positioner portion (21-3) is provided with the right positioner groove (21-3-1) that right side block lug boss (12-4-3) of opening shape and base (1) to the right is complementary;
Left side block lug boss (12-3-3) of the left side block (12-3) of base (1) is positioned at the left positioner groove (21-2-1) of the left positioner portion (21-2) of pedestal (21), and right side block lug boss (12-4-3) of the right side block (12-4) of base (1) is positioned at the right positioner groove (21-3-1) of the right positioner portion (21-3) of pedestal (21);
The top of rear side block (12-2) is printed on the corresponding mark in position with each vertical holder of arranging (13) from left to right successively; The top of left side block (12-3) is printed on the corresponding mark in position with the holder (13) of each horizontally-arranged; The right front at the top of front side block (12-1) is provided with breach (12-1-1).
8. according to the described removable albuminous membranae chip of claim 7, it is characterized in that: said base (1) also comprises side plate (14); Side plate (14) has 2, is divided into left plate (14-1) and right plate (14-2); Left plate (14-1) is connected on the base plate (11) from the top by its lower end, and is positioned at the left side central portion of base plate (11); The left side central portion of left plate (14-1) is provided with protruding left plate Access Division (14-1-1) left; Right plate (14-2) is connected on the base plate (11) from the top by its lower end, and is positioned at the right side central of base plate (11); The right side central of right plate (14-2) is provided with protruding right plate Access Division (14-2-1) to the right; Left side block (12-3) is divided into the left side block leading portion (12-3-1) that is positioned at left plate (14-1) the place ahead and is positioned at the left side block back segment (12-3-2) at left plate (14-1) rear; Right side block (12-4) is divided into the right side block leading portion (12-4-1) that is positioned at right plate (14-2) the place ahead and is positioned at the right side block back segment (12-4-2) at right plate (14-2) rear.
9. according to the described removable albuminous membranae chip of one of claim 1 to 3, it is characterized in that: the height of the cutting edge (24-3) of the loam cake (24) of said reaction bar (2) is 0.5 to 5mm; The bottom of the xsect of cutting edge (24-3) be the downward isosceles triangle of drift angle or minor face under isosceles trapezoid.
10. according to the described removable albuminous membranae chip of one of claim 1 to 3; It is characterized in that: said diaphragm (23) is a nitrocellulose membrane, and each the protein microarray zone (23-1) on the diaphragm (23) comprises 2 to 1000 protein spots sampling points (23-1-1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103214600A CN102507956A (en) | 2011-10-20 | 2011-10-20 | Detachable protein film chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103214600A CN102507956A (en) | 2011-10-20 | 2011-10-20 | Detachable protein film chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102507956A true CN102507956A (en) | 2012-06-20 |
Family
ID=46220062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103214600A Pending CN102507956A (en) | 2011-10-20 | 2011-10-20 | Detachable protein film chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102507956A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091483A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Production method of membrane diagnostic products |
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| CN2613759Y (en) * | 2003-03-31 | 2004-04-28 | 穆海东 | Protein chip |
| CN1448719A (en) * | 2003-05-13 | 2003-10-15 | 上海晶泰生物技术有限公司 | Novel biological chip |
| US7344877B1 (en) * | 2004-12-31 | 2008-03-18 | Joseph Camacho | Biomolecule microarray support |
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Application publication date: 20120620 |