CN202216955U - Protein membrane chip - Google Patents
Protein membrane chip Download PDFInfo
- Publication number
- CN202216955U CN202216955U CN2011203000062U CN201120300006U CN202216955U CN 202216955 U CN202216955 U CN 202216955U CN 2011203000062 U CN2011203000062 U CN 2011203000062U CN 201120300006 U CN201120300006 U CN 201120300006U CN 202216955 U CN202216955 U CN 202216955U
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- base
- loam cake
- boss
- substrate
- pad
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Abstract
The utility model relates to a protein membrane chip which comprises a base, a gasket, a membrane and an upper cover, wherein base holes are formed on the base, gasket holes are formed on the gasket, the lower end face of the gasket is glued and fixed on the upper end face of the base from above, the lower end face of the membrane is glued and fixed on the upper end face of the gasket from above, the upper cover comprises an upper cover substrate, an upper cover boss and cutting blades, the upper cover boss is arranged at the central of the upper cover substrate and comprises an upper cover boss external frame and upper cover boss barriers, the upper cover boss barriers divide a central hole on the upper cover boss external frame into a group of upper cover holes of which both the number and the sizes are the same as the base holes, the cutting blades are arranged along the middle of the upper cover boss external frame and the middle of the upper cover boss barriers, the upper end faces of the cutting blades are connected with the bottom of the upper cover boss, the upper cover is detachably fixed on the base from above, the cutting blades of the upper cover pass through the membrane and are inserted into the gasket, and the membrane is cut to protein microarray regions. The protein membrane chip is convenient to assemble, and cross contamination of samples can be avoided.
Description
Technical field
The utility model relates to a kind of biochip, especially a kind of protein-chip.
Background technology
The albuminous membranae chip is that protein is fixed on the film medium carrier in an orderly manner, and making it becomes the biochip that detects usefulness, then it is carried out the immune detection analysis.The albuminous membranae chip can use the chemiluminescence enzyme immunoassay method to carry out check and analysis; Its principle for mark the antibody of horseradish peroxidase (antigen) and chip effect, the antibody (antigen) that is complementary with protein on the chip after immune response takes place in antigen corresponding with it (antibody), formation solid-phase coating compound; After washing; Add luminous agent, the enzymatic luminous agent is luminous, goes out to measure the concentration of thing subsequently through collection light signal computable analysis.
Chinese patent document CN2497313Y (application number is 01253423.4) has announced a kind of protein chip; Comprise lid and box body; Lid is one the plate of porous to be arranged, and box body is formed by being pasted on porose pad and the porose plate that is pasted on the pad below that the pad top has film, a biadhesive of a plurality of albumen dot matrix.This protein chip can be applicable to the parallel detection of many indexs.Though the demarcation strip in each hole of this protein chip protrudes in this plastic plate above and below; And be close on the diaphragm; But because diaphragm joins together and has permeability, each hole can be that seepage takes place media each other with the diaphragm when adding reagent, causes the cross pollution between the sample.
Chinese patent document CN2577283Y (application number is 02261367.6) has announced that a kind of protein-chip comprises base, reagent, reaction chamber, patch and geometrical clamp.Base has the hole array, and reagent is positioned in the groove of base, and reaction chamber has the hole array, have on the patch with base on the corresponding convexity of hole size, shape.Each assembly separates, and stacks a time-out, and the hole array is corresponding each other.The diaphragm of this protein-chip is slit into Small diaphragm-piece with the protein array for the unit average mark; The slit is arranged between the Small diaphragm-piece; Concrete assembly operation step is that diaphragm is affixed on the adhesive faces of one of them pad, cuts along the centre of hole wall, and be that unit is divided into blockage with the protein array with diaphragm; Again second pad is affixed on the diaphragm, thereby solved the problem of cross pollution between the sample.But the assembly of this protein-chip is more, and diaphragm needs hand cut become blockage, and process is loaded down with trivial details, is inappropriate for large-scale production.
Summary of the invention
The purpose of the utility model provides a kind of simple for structure, and is easy to assembly, avoids the albuminous membranae chip of sample cross pollution.
A kind of technical scheme of realization the utility model purpose provides a kind of albuminous membranae chip and comprises base, pad, diaphragm and loam cake; Offer the base holes of the rectangular array distribution of one group of up/down perforation on the base; Pad is the foam pad that upper surface and lower surface all have viscosity; Offer one group of pad hole on the pad, the quantity in pad hole with the size all with base on base holes identical; Pad is pasted and fixed on the base upper surface from the top by its lower surface, and the position in each pad hole is all corresponding with the position of each base holes; Diaphragm is pasted and fixed on from the top on the upper surface of pad by its lower surface, on the upper surface of diaphragm with the corresponding position of each base holes be protein microarray zone;
Loam cake is an integral piece, comprises loam cake substrate, loam cake boss and cutting edge; The loam cake substrate is horizontally disposed with; The loam cake boss is arranged on the central position of loam cake substrate, and the upper surface of loam cake boss is higher than the upper surface of loam cake substrate; The loam cake boss comprises loam cake boss housing and loam cake boss jube, and loam cake boss jube is latticed; Loam cake boss jube is positioned at the medium pore of loam cake boss housing, with the medium pore of loam cake boss housing be divided into one group of quantity and size all with base on the identical loam cake hole of base holes; The position in each loam cake hole is all corresponding with the position of each base holes; Cutting edge is provided with along the middle part of loam cake boss housing and the middle part of loam cake boss jube, forms rectangular node, and is connected the bottom of loam cake boss by its upper surface; The height of cutting edge is less than or equal to the thickness of pad;
Loam cake is removable being fixed on the base from the top; The cutting edge of loam cake passes diaphragm, inserts in the pad, and diaphragm is cut into mutual disjunct protein microarray zone; The position in protein microarray zone is all corresponding with the position in each base holes and each loam cake hole.
Above-mentioned base is an integral piece, comprises base substrate and base boss; Base substrate is horizontally disposed with; Base boss is arranged on the central position of base substrate, and the upper surface of base boss is higher than the upper surface of base substrate; Base boss comprises base boss housing and base boss jube; The base boss jube is latticed, and the base boss jube is positioned at the medium pore of base boss housing, and the medium pore of base boss housing is divided into described base holes;
The shape of above-mentioned pad is identical with the shape of the upper surface of base boss; The shape of above-mentioned diaphragm is identical with the shape of the upper surface of base boss;
Above-mentioned loam cake also comprises peripheral panels; Peripheral panels vertically is provided with, and is connected the edge of loam cake substrate by its top, has surrounded the loam cake inner chamber that Open Side Down jointly with the loam cake substrate, and the shape of inner chamber and the shape of base boss are complementary;
The base boss of base stretches in the inner chamber of loam cake, and with the inner chamber wringing fit of loam cake.
4 boundaries that are positioned at the base boss outside of the base substrate of above-mentioned base respectively offer 1 to 3 fixed orifice; Fixed orifice is blind hole; Said loam cake also comprises fixed leg; The quantity of the fixed orifice on the quantity of fixed leg and the base substrate is identical; Fixed leg is connected the bottom of peripheral panels by its top, and the size and the position of each fixed orifice on the size of each root fixed leg and position and the base substrate are corresponding; Each root fixed leg of loam cake stretches into respectively in the fixed orifice of corresponding base separately, and with corresponding fixed orifice wringing fit separately.
The outline of the base boss housing of above-mentioned base is for lacking the rectangle at 1 angle; The global shape of the loam cake substrate of said loam cake is for lacking the rectangle at 1 angle.
Above-mentioned peripheral panels respectively offer 2 grooves that Open Side Down on 4 end faces all around.
The base boss jube of above-mentioned base comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions; Each the piece longitudinal baffle of base boss jube and the two ends of each piece lateral partitions all link to each other with the inwall of base boss housing, and the medium pore of base boss housing is divided into 48 base holes; 48 base holes are square, and rectangular array distribution totally 6 is arranged 8 of every rows;
The loam cake boss jube of above-mentioned loam cake comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions; Each the piece longitudinal baffle of loam cake boss jube and the two ends of each piece lateral partitions all link to each other with the inwall of loam cake boss housing, and the medium pore of loam cake boss housing is divided into 48 loam cake holes.
The height of the cutting edge of above-mentioned loam cake is 0.5 to 5mm, and the thickness of above-mentioned diaphragm is 0.5 to 5mm; The top of the xsect of cutting edge is rectangle, and the bottom of its xsect is the downward isosceles triangle of drift angle.
The bottom of the xsect of the cutting edge of above-mentioned loam cake is the isosceles right triangle of down at right angles.
Protein microarray zone on the above-mentioned diaphragm comprises 2 to 1000 protein spots sampling points.
The utlity model has positive effect:
(1) the albuminous membranae chip of the utility model is cut each protein microarray zone of diaphragm open through cutting edge when assembling; Be convenient to assembling; Effectively raise this production efficiency of products and yield rate, fundamentally avoided the cross pollution between the sample.
(2) each protein microarray zone passage chip loam cake of the albuminous membranae chip of the utility model has been separated into one by one independently unit, thereby can realize many indexs, many person-portions, parallelization disease detection.
(3) below in each protein microarray of the albuminous membranae chip of the utility model zone corresponding the base holes of chip pad, this below hollow out be designed with the cleaning that is beneficial to chip.
(4) peripheral panels of the loam cake of the albuminous membranae chip of the utility model be equipped with groove on 4 end faces all around, be convenient to adopt location and the extracting of self-reacting device to the albuminous membranae chip.
Description of drawings
Fig. 1 is the schematic perspective view of a kind of albuminous membranae chip among the utility model embodiment 1;
Fig. 2 is the perspective exploded view of Fig. 1;
Fig. 3 is loam cake among Fig. 2 structural representation during from beneath;
Fig. 4 is the structural representation after the local amplification in the B place among Fig. 3;
Fig. 5 is the cut-away view of the loam cake A-A face among Fig. 2;
Fig. 6 is the structural representation after the local amplification in the C place among Fig. 5.
The Reference numeral of above-mentioned accompanying drawing is following:
Base 1, base substrate 11, fixed orifice 11-1, base boss 12, base boss housing 12-1, base boss jube 12-2, base holes 12-3,
Pad 2, pad hole 21,
Diaphragm 3, protein microarray zone 31, protein spots sampling point 31-1,
Embodiment
(embodiment 1)
See Fig. 1 and Fig. 2, a kind of albuminous membranae chip of present embodiment comprises base 1, pad 2, diaphragm 3 and loam cake 4.
Base 1 is the integrated through injection molding part, comprises base substrate 11 and base boss 12.Base substrate 11 is horizontally disposed with, and global shape is 4 rectangles that the angle is a fillet.Base boss 12 is arranged on the central position of base substrate 11, and the upper surface of base boss 12 is higher than the upper surface of base substrate 11.4 boundaries that are positioned at base boss 12 outsides of base substrate 11 offer 12 fixed orifice 11-1, and every boundary is provided with 3.Fixed orifice 11-1 is blind hole, and 3 fixed orifice 11-1 of every boundary equidistantly distribute.
Base boss 12 comprises base boss housing 12-1 and base boss jube 12-2.The outline of base boss housing 12-1 is for lacking the rectangle at 1 angle.Base boss jube 12-2 is latticed, comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions.Base boss jube 12-2 is positioned at the medium pore of base boss housing 12-1; And each the piece longitudinal baffle of base boss jube 12-2 and the two ends of each piece lateral partitions all link to each other with the inwall of base boss housing 12-1, thereby the medium pore of base boss housing 12-1 is divided into 48 up/down perforations and base holes 12-3 of the same size.48 base holes 12-3 are square, and rectangular array distribution totally 6 is arranged 8 of every rows.
Pad 2 is the foam pads that all have viscosity by the upper surface of 3M company production and lower surface, and thickness is 2mm.The shape of pad 2 is identical with the shape of the upper surface of base boss 12.Offer 48 pad holes 21 on the pad 2.The size in pad hole 21 is identical with base holes 12-3.Pad 2 is pasted and fixed on from the top by its lower surface on the upper surface of base boss 12 of base 1, and the position in each pad hole 21 is all corresponding with the position of each base holes 12-3.
Diaphragm 3 is a nitrocellulose membrane, and this nitrocellulose membrane is that the preparation method through the disclosed a kind of protein-chip of Chinese patent document CN100341570C (patent No. is ZL03116076.X) makes.The shape of diaphragm 3 is identical with the shape of the upper surface of base boss 12.Diaphragm 3 is pasted and fixed on from the top on the upper surface of pad 2 by its lower surface, on the upper surface of diaphragm 3 with the corresponding position of each base holes 12-3 be protein microarray zone 31.Each protein microarray zone 31 comprises 10 protein spots sampling point 31-1.
See Fig. 2, Fig. 3 and Fig. 5, loam cake 4 is the integrated through injection molding part, comprises loam cake substrate 41, loam cake boss 42, peripheral panels 43, fixed leg 44 and cutting edge 45.
Loam cake boss 42 comprises loam cake boss housing 42-1 and loam cake boss jube 42-2.The outline of loam cake boss housing 42-1 is a rectangle.Loam cake boss jube 42-2 is latticed, comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions.Loam cake boss jube 42-2 is positioned at the medium pore of loam cake boss housing 42-1; And each the piece longitudinal baffle of loam cake boss jube 42-2 and the two ends of each piece lateral partitions all link to each other with the inwall of loam cake boss housing 42-1; Thereby with the medium pore of loam cake boss housing 42-1 be divided into 48 up/down perforations and with base holes 12-3 loam cake of the same size hole 42-3, the position of each loam cake hole 42-3 is all corresponding with the position of each base holes 12-3.Cutting edge 45 is provided with along the middle part of loam cake boss housing 42-1 and the middle part of loam cake boss jube 42-2, forms rectangular node, and is connected the bottom of loam cake boss 42 by its upper surface.The height of cutting edge 45 is 1.8mm, and the top of the xsect of cutting edge 45 is rectangle, and the bottom of its xsect is the isosceles right triangle (seeing Fig. 3 to Fig. 6 for details) of down at right angles.
Each root fixed leg 44 of loam cake 4 stretches into respectively among the fixed orifice 11-1 of corresponding separately base 1, and with corresponding fixed orifice 11-1 wringing fit separately; The base boss 12 of base 1 stretches in the inner chamber of loam cake 4, and with the inner chamber wringing fit of loam cake 4, thereby make loam cake 4 from the top removable being fixed on the base 1.
The cutting edge 45 of loam cake 4 passes diaphragm 3; Insert in the pad 2; Thereby each protein microarray zone 31 of diaphragm 3 is cut open; Form 48 mutual disjunct protein microarray zones 31, and the position in each piece protein microarray zone 31 is all corresponding with the position of each base holes 12-3 and each loam cake hole 42-3.
Claims (9)
1. an albuminous membranae chip is characterized in that: comprise base (1), pad (2), diaphragm (3) and loam cake (4); Offer the base holes (12-3) of the rectangular array distribution of one group of up/down perforation on the base (1); Pad (2) is the foam pad that upper surface and lower surface all have viscosity; Offer one group of pad hole (21) on the pad (2), the quantity of pad hole (21) with the size all with base (1) on base holes (12-3) identical; Pad (2) is pasted and fixed on base (1) upper surface from the top by its lower surface, and the position in each pad hole (21) is all corresponding with the position of each base holes (12-3); Diaphragm (3) is pasted and fixed on from the top on the upper surface of pad (2) by its lower surface, on the upper surface of diaphragm (3) with the corresponding position of each base holes (12-3) be protein microarray zone (31);
Loam cake (4) is an integral piece, comprises loam cake substrate (41), loam cake boss (42) and cutting edge (45); Loam cake substrate (41) is horizontally disposed with; Loam cake boss (42) is arranged on the central position of loam cake substrate (41), and the upper surface of loam cake boss (42) is higher than the upper surface of loam cake substrate (41); Loam cake boss (42) comprises loam cake boss housing (42-1) and loam cake boss jube (42-2), and loam cake boss jube (42-2) is latticed; Loam cake boss jube (42-2) is positioned at the medium pore of loam cake boss housing (42-1), with the medium pore of loam cake boss housing (42-1) be divided into one group of quantity and size all with base (1) on the identical loam cake hole (42-3) of base holes (12-3); The position in each loam cake hole (42-3) is all corresponding with the position of each base holes (12-3); Cutting edge (45) is provided with along the middle part of loam cake boss housing (42-1) and the middle part of loam cake boss jube (42-2), forms rectangular node, and is connected the bottom of loam cake boss (42) by its upper surface; The height of cutting edge (45) is less than or equal to the thickness of pad (2);
Loam cake (4) is removable being fixed on the base (1) from the top; The cutting edge (45) of loam cake (4) passes diaphragm (3), inserts in the pad (2), and diaphragm (3) is cut into mutual disjunct protein microarray zone (31); The position in protein microarray zone (31) is all corresponding with the position in each base holes (12-3) and each loam cake hole (42-3).
2. according to the described a kind of albuminous membranae chip of claim 1, it is characterized in that: said base (1) is an integral piece, comprises base substrate (11) and base boss (12); Base substrate (11) is horizontally disposed with; Base boss (12) is arranged on the central position of base substrate (11), and the upper surface of base boss (12) is higher than the upper surface of base substrate (11); Base boss (12) comprises base boss housing (12-1) and base boss jube (12-2); Base boss jube (12-2) is latticed, and base boss jube (12-2) is positioned at the medium pore of base boss housing (12-1), and the medium pore of base boss housing (12-1) is divided into described base holes (12-3);
The shape of said pad (2) is identical with the shape of the upper surface of base boss (12); The shape of said diaphragm (3) is identical with the shape of the upper surface of base boss (12);
Said loam cake (4) also comprises peripheral panels (43); Peripheral panels (43) vertically is provided with, and is connected the edge of loam cake substrate (41) by its top, has surrounded loam cake (4) inner chamber that Open Side Down jointly with loam cake substrate (41), and the shape of the shape of inner chamber and base boss (42) is complementary;
The base boss (12) of base (1) stretches in the inner chamber of loam cake (4), and with the inner chamber wringing fit of loam cake (4).
3. according to the described a kind of albuminous membranae chip of claim 2, it is characterized in that: 4 boundaries that are positioned at base boss (12) outside of the base substrate (11) of said base (1) respectively offer 1 to 3 fixed orifice (11-1); Fixed orifice (11-1) is blind hole; Said loam cake (4) also comprises fixed leg (44); The quantity of the fixed orifice (11-1) on the quantity of fixed leg (44) and the base substrate (11) is identical; Fixed leg (44) is connected the bottom of peripheral panels (43) by its top, and the size and the position of each fixed orifice (11-1) on the size of each root fixed leg (44) and position and the base substrate (11) are corresponding; Each root fixed leg (44) of loam cake (4) stretches into respectively in the fixed orifice (11-1) of corresponding separately base (1), and with corresponding fixed orifice (11-1) wringing fit separately.
4. according to the described a kind of albuminous membranae chip of one of claim 3, it is characterized in that: the outline of the base boss housing (12-1) of said base (1) is for lacking the rectangle at 1 angle; The global shape of the loam cake substrate (41) of said loam cake (4) is for lacking the rectangle at 1 angle.
5. according to the described a kind of albuminous membranae chip of one of claim 4, it is characterized in that: said peripheral panels (43) respectively offer 2 grooves that Open Side Down (43-1) on 4 end faces all around.
6. according to the described a kind of albuminous membranae chip of claim 5, it is characterized in that: the base boss jube (12-2) of said base (1) comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions; Each the piece longitudinal baffle of base boss jube (12-2) and the two ends of each piece lateral partitions all link to each other with the inwall of base boss housing (12-1), and the medium pore of base boss housing (12-1) is divided into 48 base holes (12-3); 48 base holes (12-3) are square, and rectangular array distribution totally 6 is arranged 8 of every rows;
The loam cake boss jube (42-2) of said loam cake (4) comprises 7 longitudinal baffles and 5 lateral partitions, and the width of each piece longitudinal baffle is identical with the width of each piece lateral partitions; Each the piece longitudinal baffle of loam cake boss jube (42-2) and the two ends of each piece lateral partitions all link to each other with the inwall of loam cake boss housing (42-1), and the medium pore of loam cake boss housing (42-1) is divided into 48 loam cake holes (42-3).
7. according to the described a kind of albuminous membranae chip of one of claim 1 to 6, it is characterized in that: the height of the cutting edge (45) of said loam cake (4) is 0.5 to 5mm, and the thickness of said diaphragm is 0.5 to 5mm; The top of the xsect of cutting edge (45) is rectangle, and the bottom of its xsect is the downward isosceles triangle of drift angle.
8. according to the described albuminous membranae chip of claim 7, it is characterized in that: the bottom of the xsect of the cutting edge (45) of said loam cake (4) is the isosceles right triangle of down at right angles.
9. according to the described a kind of albuminous membranae chip of one of claim 1 to 6, it is characterized in that: the protein microarray zone (31) on the said diaphragm (3) comprises 2 to 1000 protein spots sampling points (31-1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203000062U CN202216955U (en) | 2011-08-17 | 2011-08-17 | Protein membrane chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203000062U CN202216955U (en) | 2011-08-17 | 2011-08-17 | Protein membrane chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202216955U true CN202216955U (en) | 2012-05-09 |
Family
ID=46016203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203000062U Expired - Lifetime CN202216955U (en) | 2011-08-17 | 2011-08-17 | Protein membrane chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202216955U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323429A (en) * | 2011-08-17 | 2012-01-18 | 上海裕隆生物科技有限公司 | Protein membrane chip |
-
2011
- 2011-08-17 CN CN2011203000062U patent/CN202216955U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323429A (en) * | 2011-08-17 | 2012-01-18 | 上海裕隆生物科技有限公司 | Protein membrane chip |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shanghai incorporated company of Yue Loong medical test institute Assignor: Shanghai Yulong Biological Science And Technology Co., Ltd. Contract record no.: 2014310000065 Denomination of utility model: Protein membrane chip Granted publication date: 20120509 License type: Exclusive License Record date: 20140409 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120509 |
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| CX01 | Expiry of patent term |