CN102453647A - Pre-treatment method for medlar fermentation wine - Google Patents
Pre-treatment method for medlar fermentation wine Download PDFInfo
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- CN102453647A CN102453647A CN201010515118XA CN201010515118A CN102453647A CN 102453647 A CN102453647 A CN 102453647A CN 201010515118X A CN201010515118X A CN 201010515118XA CN 201010515118 A CN201010515118 A CN 201010515118A CN 102453647 A CN102453647 A CN 102453647A
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Abstract
The present invention relates to a pre-treatment method for a medlar fermentation wine. The method comprises: removing protein in the medlar juice, and inoculating yeast to carry out alcoholic fermentation. With the method of the present invention, the most of the protein in the medlar juice can be removed, such that the content of the higher alcohol generated during the alcoholic fermentation process with the yeast inoculating is reduced so as to improve the quality of the medlar fermentation wine, and release and improve headache and other adverse symptoms caused by the raw material problem after drinking the medlar wine.
Description
Technical field
The invention belongs to food-processing and biological fermentation field, particularly, the present invention relates to a kind of pre-treating process of fermentation Chinese wolfberry fruit wine.
Background technology
The fermentation Chinese wolfberry fruit wine amount of drinking is big slightly, and drinking person promptly reflects the symptom of headache.No matter which kind of wine body when the higher alcohols too high levels, all can produce toxic action to human body.Higher alcohols is along with molecular weight increases and the toxicity increase.Primary isoamyl alcohol particularly when its content is high, makes nerve system of human body congested after the drink, produce headache, feel sick, vomiting etc., and unsuitable volume exists.See that from the mechanism of production of higher alcohols the generation of higher alcohols is main relevant with amino acid whose metabolism, and the relation of being proportionate.The protein contnt of Sucus Vitis viniferae is 0.5%; The protein contnt of dry wolfberry is up to 13%～21%, even fresh fructus lycii juice protein content also up to 3%, is 5～6 times of Sucus Vitis viniferae protein contnt; So the protein contnt height is to be the high major cause of fermented wine higher alcohols content of raw material with the matrimony vine.Now wine fermentation all adopts traditional wine fermentation method, promptly raw material through damaged, go into a jar fermentation, after method such as ferment form.Should according to raw material separately different features carry out pre-treatment, to produce the fruit wine product that conforms to quality requirements.So how reducing in the matrimony vine juice protein contnt is to solve high this key of problem of fermentation Chinese wolfberry fruit wine higher alcohols content.
Therefore, this area needs the fermentation Chinese wolfberry fruit wine pre-treating process, removing most protein in the matrimony vine juice effective and easy and simple to handlely, thereby reduces the higher alcohols content that produces when inoculation yeast is carried out zymamsis.
Summary of the invention
The object of the present invention is to provide a kind of raw material pre-treating process that reduces fermentation Chinese wolfberry fruit wine higher alcohols content; Use this method; Can remove most protein in the matrimony vine juice easy and effectively; Thereby reduce the higher alcohols content that produces when inoculation yeast is carried out zymamsis, and then improve the quality of fermentation Chinese wolfberry fruit wine, alleviate and improve ill symptomses such as the matrimony vine beverage back of causing because of the raw material problem is painful.
In order to realize the object of the invention, the present invention provides a kind of pre-treating process of fermentation Chinese wolfberry fruit wine, and said method comprises removes proteinic step in the matrimony vine juice.Preferably, proteinic step is accomplished through following glue or ultrafiltration mode in the said removal matrimony vine juice.
In a preferred embodiment, the present invention provides a kind of pre-treating process of fermentation Chinese wolfberry fruit wine, and said method comprises through following glue removes proteinic step in the matrimony vine juice.
In another preferred embodiment, the present invention provides a kind of pre-treating process of fermentation Chinese wolfberry fruit wine, and said method comprises through ultrafiltration removes proteinic step in the matrimony vine juice.
According to one embodiment of the invention, the pre-treating process of said fermentation Chinese wolfberry fruit wine comprises that the matrimony vine juice that obtains is carried out pectin degrading earlier to be handled, and plays glue to handle then, inoculates yeast and carries out zymamsis.In a preferred embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises that the matrimony vine juice that obtains is carried out pectin degrading earlier to be handled, and removes the throw out that enzymolysis forms afterwards, play glue to handle after again, and inoculation yeast is carried out zymamsis at last.Carrying out the pectin degrading processing before the glue down, also can not carry out pectin degrading and handle.In another preferred embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention is removed down the throw out that glue forms after being included in down the glue processing, and then carries out the inoculation yeast fermentation.
In a concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, following glue and inoculation fermentation step.In another concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, and following glue filters and the inoculation fermentation step.In another concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, and filters, and following glue filters and the inoculation fermentation step.
In another embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises that the matrimony vine juice that obtains is carried out pectin degrading earlier to be handled, and carries out uf processing then, inoculates yeast and carries out zymamsis.In preferred embodiments, said uf processing is carried out ultrafiltration through ultra-filtration membrane and is carried out.In a preferred embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises that the matrimony vine juice that obtains is carried out pectin degrading earlier to be handled, and removes the throw out that enzymolysis forms afterwards, and then carries out uf processing.
In a concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, ultrafiltration and inoculation fermentation step.In another concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, coarse filtration, ultrafiltration and inoculation fermentation step.In another concrete embodiment, the pre-treating process of fermentation Chinese wolfberry fruit wine of the present invention comprises the matrimony vine making beating successively, and polygalacturonase is handled, and filters ultrafiltration and inoculation fermentation step.
As defined herein; Following glue is meant according to contained physical property in the pending liquid; Adopt finingss such as gelatin, chitosan, egg white, wilkinite, tannin to treat treatment solution and carry out clarifying treatment, make its with pending liquid in tannin, protein, metal composite, and generation throwing out such as pectin substance; Thereby these materials are removed, with the clarity that improves pending liquid, stability etc.
The colloid material that the present invention uses can be any colloid material in this area.The instance of said colloid material is wilkinite, tannin, gelatin, chitosan, egg-white powder etc.Preferably, employed colloid material is wilkinite or tannin among the present invention, thereby a large amount of protein that absorption removes in the matrimony vine to be contained obtain fabulous effect.
Ultra-filtration membrane used herein can be any suitable ultra-filtration membrane.The representative instance of ultra-filtration membrane of the present invention is conventional organic ultra-filtration membrane or inorganic ceramic ultra-filtration membrane, and preferred molecular weight cut-off is 30kDa～50kDa.
Zymamsis of the present invention is conventional wine fermentation method, non-solid fermentation.
Advantage of the present invention is the pre-treating process that has been to increase fermentation Chinese wolfberry fruit wine, through the mode of glue or ultrafiltration down the most of protein in the matrimony vine juice is removed.
The present invention also provides the preparation method of fermentation Chinese wolfberry fruit wine, and it comprises through fermentation Chinese wolfberry fruit wine pre-treating process of the present invention removes proteinic step.
The present invention also provides fermentation Chinese wolfberry fruit wine, and it is that preparation method through fermentation Chinese wolfberry fruit wine of the present invention obtains, and the preparation method of wherein said Qi fermented wine comprises through fermentation Chinese wolfberry fruit wine pre-treating process of the present invention and removes proteinic step.
In a concrete embodiment, fermentation Chinese wolfberry fruit wine of the present invention obtains through process for preparation of fermentation Chinese wolfberry fruit wine of the present invention, and said method comprises the steps:
1. obtain to remove proteinic matrimony vine juice according to above-mentioned fermentation Chinese wolfberry fruit wine pre-treating process; With
2. utilize matrimony vine juice that step 1 obtains to prepare fermentation Chinese wolfberry fruit wine according to the conventional preparation method of fermentation Chinese wolfberry fruit wine.
Through method of the present invention; Can remove most protein in the matrimony vine juice; Thereby produce a large amount of higher alcoholss through the amino acid metabolism approach when having avoided yeast to carry out zymamsis, improve wine body quality, alleviate and improve ill symptomses such as the matrimony vine beverage back of causing because of the raw material problem is painful.
Description of drawings
Fig. 1 is the schema of the pre-treating process of typical fermentation Chinese wolfberry fruit wine of the present invention;
Fig. 2 is the schema of the pre-treating process of typical fermentation Chinese wolfberry fruit wine of the present invention, wherein after polygalacturonase is handled, through removing by filter the throw out that enzymolysis forms, and then play glue or uf processing, carries out inoculation yeast again and ferments;
Fig. 3 is the schema of the pre-treating process of typical fermentation Chinese wolfberry fruit wine of the present invention, wherein after polygalacturonase is handled, through coarse filtration; Carry out ultrafiltration then, carry out the inoculation yeast fermentation again, after perhaps polygalacturonase is handled; Following glue removes by filter down the glue throw out then, carries out the inoculation yeast fermentation again.
Embodiment
Mode through embodiment is further described the present invention, but the present invention is not limited only to following examples.
Employed matrimony vine is from China peaceful Xia Zhongning green food base in following examples; Polygalacturonase is a jade of the He family board polygalacturonase, from Ningxia jade of the He family Bioisystech Co., Ltd; Wilkinite is from comprehensive rock ore deposit, Chinese Xi'an test center mineral chemical institute.Other materials and reagent are the conventional products of commercially available acquisition.
Embodiment 1
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.03g/kg, insulation is to 60 ℃; Enzyme was handled after 60 minutes, added in advance the fully wilkinite of water-swelling, stirred; Add-on forms post precipitation inoculation yeast bacterium and carries out zymamsis in dry product 1.8g/L.
Embodiment 2
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.035g/kg, insulation is to 50 ℃; Enzyme was handled after 90 minutes; Add the wilkinite of in advance abundant water-swelling, stir, add-on is in dry product 1.8g/L; With diatomite filter (Qilu Packing Machine Co., Ltd., Qingzhou, model: GLW250) remove by filter down the post precipitation inoculation yeast bacterium that glue forms and carry out zymamsis.
Embodiment 3
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 55 ℃; Enzyme was handled after 100 minutes; Remove by filter throw out, add in advance the fully wilkinite of water-swelling, stir; Add-on is in dry product 2.0g/L, and the post precipitation inoculation yeast bacterium that removes by filter down glue formation carries out zymamsis.
Embodiment 4
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 55 ℃; Enzyme was handled after 90 minutes, with 0.45 micron ceramic membrane filter machine (the outstanding film engineering of Hefei generation Ltd, product type: SJM-FHM-0.01) filter; Remove the macrobead pomace; Adopting molecular weight cut-off then is that organic ultra-filtration membrane (available from the outstanding film of Hefei generation Engineering Co., Ltd, China) of 30kDa is handled, and the inoculation yeast bacterium carries out zymamsis then.
Embodiment 5
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 55 ℃; Enzyme was handled after 90 minutes; Remove by filter throw out, adopting molecular weight cut-off then is that organic ultra-filtration membrane of 50kDa is handled, and the inoculation yeast bacterium carries out zymamsis then.
Embodiment 6
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 50 ℃, and enzyme was handled after 90 minutes, and the employing molecular weight cut-off is that the inorganic ultra-filtration membrane of 30kDa is handled, and the inoculation yeast bacterium carries out zymamsis then.
Embodiment 7
With obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 50 ℃, and enzyme was handled after 90 minutes, and the employing molecular weight cut-off is that the inorganic ultra-filtration membrane of 50kDa is handled, and the inoculation yeast bacterium carries out zymamsis then.
Embodiment 8
With obtaining matrimony vine juice after the matrimony vine making beating, without the polygalacturonase processing, directly in matrimony vine juice, add in advance the fully wilkinite of water-swelling, stir, add-on is in dry product 2.0g/L, and the post precipitation inoculation yeast bacterium that removes by filter down glue formation carries out zymamsis.
Embodiment 9
Be metered to jar behind the abundant mixing of matrimony vine juice that will prepare through the method for above embodiment; The polygalacturonase that adds 0.04g/kg was handled 2 hours under 60 ℃ condition; Add the 0.8g/L tannin then; The aftertreatment 2 hours that stirs, with 0.2 μ m ceramic membrane filter, the back is detected physical and chemical index and is metered to jar and carries out temperature controlled fermentation.
Embodiment 10
Present embodiment is the experiment that fermentation Chinese wolfberry fruit wine that the fermentation Chinese wolfberry fruit wine for preparing after the treatment process through above embodiment 1-8 and process for preparation of fermentation Chinese wolfberry fruit wine of the prior art obtain compares.
The sample of embodiment 1-8 is through the method preparation of above embodiment 1-8.
The preparation method of control samples 1: with obtaining matrimony vine juice after the matrimony vine making beating, in matrimony vine juice, add polygalacturonase 0.04g/kg, insulation is to 50 ℃, and enzyme was handled after 90 minutes, removed by filter post precipitation inoculation yeast bacterium and carried out zymamsis, and the sample that obtains is control sample.
The preparation method of control samples 2: with obtaining matrimony vine juice after the matrimony vine making beating, the inoculation yeast bacterium directly carries out zymamsis in matrimony vine juice, and the sample that obtains is control sample 2.
The comparison of the fermentation Chinese wolfberry fruit wine of table 1 prepared according to the methods of the invention fermentation Chinese wolfberry fruit wine and ordinary method preparation
More than experiment all repeats 3 times.
Through detecting, the protein contnt of the fermentation Chinese wolfberry fruit wine that obtains through treatment process of the present invention is reduced to about 0.21-0.23g/100ml, the about 92.2-92.8% of protein decreasing ratio by the about 2.90-2.94g/100ml before handling; The about 0.32-0.38g/l of wine body volatile acid, conformance with standard (standard value is less than 1.0g/l), potato spirit is reduced to about 0.001-0.0013g/l by about 0.037-0.039g/l, reduces by 97%.
Judge experimental record:
The fermentation Chinese wolfberry fruit wine of judging of the present invention is the fermentation Chinese wolfberry fruit wine through the method preparation of the foregoing description 1-8.Control sample 1 among the preparation method of control sample and the embodiment 10 is identical with the preparation method of control sample 2.
The person of sampling wine is the male sex, and the age, state of health was good between 25-55 year, history of drinking history 2-40, and basic liquor capacity for liquor is at 0.5-1.0 jin.The person of sampling wine is divided into not on the same group at random every group 8 people.
The experiment of sampling wine shows that through the fermentation Chinese wolfberry fruit wine good palatability of pre-treating process of the present invention preparation, mouthfeel is pure, and is chippy, but has other uncomfortable reaction, and contrast wine appearance little hardship that enters the mouth is drunk the back dry, headache next day.
The drunk experimental record 1 of table 3
The drunk experimental record 2 of table 4
Drunk experiment shows, better through the fermentation Chinese wolfberry fruit wine palatability of pre-treating process preparation of the present invention, mouthfeel is pure, and is chippy, do not have other uncomfortable reaction, and contrast wine appearance little hardship that enters the mouth is drunk the back dry mouth and tougue, headache next day, and it is slow to sober up.Sample wine and drunk test-results demonstration, fermentation Chinese wolfberry fruit wine of the present invention obviously is superior to contrasting fermentation Chinese wolfberry fruit wine.
Though specific embodiments of the present invention is described, those skilled in the art will appreciate that under the prerequisite that does not depart from scope of the present invention or spirit and can carry out multiple change and modification to the present invention.Thereby, this invention is intended to contain all these changes and modification of dropping in Rights attached thereto claim and the coordinator scope thereof.
Claims (10)
1. the pre-treating process of fermentation Chinese wolfberry fruit wine, said method comprise removes proteinic step in the matrimony vine juice.
2. the pre-treating process of fermentation Chinese wolfberry fruit wine according to claim 1, the proteinic step in the wherein said removal matrimony vine juice are handled through glue down and are carried out.
3. the pre-treating process of fermentation Chinese wolfberry fruit wine according to claim 1, the proteinic step in the wherein said removal matrimony vine juice are to handle through matrimony vine juice being carried out pectin degrading earlier, carry out with uf processing then.
4. the pre-treating process of fermentation Chinese wolfberry fruit wine according to claim 2, wherein said glue are down handled removing matrimony vine juice and after polygalacturonase is degraded formed throw out, are carried out.
5. fermentation Chinese wolfberry fruit wine pre-treating process according to claim 2 wherein after playing glue to handle, will be removed through the throw out of glue processing formation down.
6. according to each described fermentation Chinese wolfberry fruit wine pre-treating process in the claim 2,4 and 5, wherein playing glue to handle the material that uses is wilkinite or tannin.
7. fermentation Chinese wolfberry fruit wine pre-treating process according to claim 3, the throw out that wherein before said uf processing, earlier the polygalacturonase degraded is formed is removed.
8. according to claim 3 or 7 described fermentation Chinese wolfberry fruit wine pre-treating processs, wherein said uf processing uses ultra-filtration membrane to carry out.
9. fermentation Chinese wolfberry fruit wine pre-treating process according to claim 8, the molecular weight cut-off of wherein said ultra-filtration membrane are between the 30kDa-50kDa.
10. fermentation Chinese wolfberry fruit wine, it is that preparation method through fermentation Chinese wolfberry fruit wine obtains, the preparation method of said fermentation Chinese wolfberry fruit wine may further comprise the steps:
A. according to each described fermentation Chinese wolfberry fruit wine pre-treating process among the claim 1-9 matrimony vine juice being removed protein handles; With
B. inoculation yeast is carried out zymamsis.
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| CN104232400A (en) * | 2014-08-02 | 2014-12-24 | 宁夏红中宁枸杞制品有限公司 | Brewing method for wolfberry dry red wine |
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| CN107904074A (en) * | 2017-12-01 | 2018-04-13 | 刘滨 | A kind of preparation method of morat |
| CN108004083A (en) * | 2017-12-04 | 2018-05-08 | 四川省食品发酵工业研究设计院 | A kind of brewage process of prickly-pear cactus brandy |
| CN110885730A (en) * | 2019-12-10 | 2020-03-17 | 宁夏红枸杞产业有限公司 | Production method of Chinese wolfberry fruit wine |
| CN110951570A (en) * | 2019-12-10 | 2020-04-03 | 宁夏红枸杞产业有限公司 | Production method of powder type medlar product |
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Application publication date: 20120516 |