CN102445471B

CN102445471B - Electrochemical sensing specimen

Info

Publication number: CN102445471B
Application number: CN201010501028.5A
Authority: CN
Inventors: 徐振腾; 萧宏展
Original assignee: HUAGUANG BIOTECHNOLOGY CO Ltd
Current assignee: HUAGUANG BIOTECHNOLOGY CO Ltd; Bionime Corp
Priority date: 2010-10-09
Filing date: 2010-10-09
Publication date: 2014-04-09
Anticipated expiration: 2030-10-09
Also published as: CN102445471A

Abstract

The invention relates to an electrochemical sensing specimen, which comprises a shell unit, an electrode units respectively arranged in the shell unit, a layer of reagent reaction layer and a filtering element. The shell unit comprises an insulating base plate and a cover plate which are mutually matched to define an accommodating space, an opening part and a guiding channel. The insulating base plate is provided with an inner groove face matched to from the accommodating space, and a plurality of through holes formed on the inner groove face. The electrode units are respectively arranged in the through holes and are respectively provided with at least one sunken top surface being lower than the inner groove face. The filtering element is extended into the accommodating space through the guiding channel from the opening part, and is covered on partial reagent reaction layer and partial electrode unit. Therefore, liquid to be tested is guided to reach the reagent reaction layer quickly, the reaction time is shortened, and the sensing efficiency of the electrochemical sensing specimen is improved.

Description

Electrochemical sensing chip

Technical field

The present invention relates to a kind of sensing test piece, particularly relate to the electrochemical sensing chip that a kind of Throwing abandons formula.

Background technology

Electrochemical sensing chip (Electrochemical Sensor Strip) has been widely used in the detection of various fluids, its basic framework mainly comprises one and is fixed on this and takes up in container and can treat with this chemical reagent (Reagent) layer of the particular analysis deposits yields electrochemical action of fluid measured for accommodating the take up container, one deck for the treatment of fluid measured and having an opening, and one is arranged on this and takes up the electrode system in container.Wherein, this chemical reagent with after the analyte effect in fluid measured, can produce the output signal of an electrical parameter, by the output valve of this electrical parameter, can obtain this and treat the analyte concentration in fluid measured, this electrode system has one to electrode (counter Electrode), a working electrode (Working Electrode), a reference electrode (Reference Electrode) and a detecting electrode (Detecting Electrode).Wherein, by change, be fixed on this and take up the chemical reagent kind in container, just can make the electrochemical sensing chip of the different tested objects such as heavy metal, agricultural chemicals and poisonous chemical composition that are applicable in blood sugar, uric acid, cholesterol concentration and sewage.

In order obtaining, to detect more accurately result, conventionally can first to remove the material for the treatment of may interfere with in fluid measured detecting result.Take whole blood as example, because red blood cell can have influence on sensing result, opening and the chemical reagent interlayer that conventionally can take up container at this arrange a filtering membrane, so that the blood plasma in whole blood is separated with red blood cell and then filtering red blood cell, for example, United States Patent (USP) the 5th, 139, openly utilize glass fiber filter No. 685, just can not need to make blood cell and separating plasma centrifugal in the situation that, but when test blood volume is less, easily the adsorptive power because of filter membrane itself causes the liquid leaching very few, therefore, blood cell in the complete filtering whole blood of the ability of must exerting pressure again, but easily cause under pressure haemolysis and cause detecting result inaccurate.In addition, the adsorption between blood plasma and filter membrane also can cause the pace of blood plasma slack-off, cause need to be more of a specified duration time could obtain response result.

Consult Fig. 1, as United States Patent (USP) the 6th, 319, No. 719 disclosed electrochemical sensing chips 1, by treating to arrange in runner that fluid measured can pass through spaced at this and being generally half-moon-shaped several first and stopping that projection 11, several second stops that projection 12, the several the 3rd stops that projection 13 is as blocking and the erythrocytic structure removing in whole blood, and mainly utilize spatial structure to form and block erythrocytic obstacle, and make blood plasma part can be rapidly by described, stop that the gap of 11,12,13 of projections advances towards a reagent reacting layer 14.But this kind of design needs larger flow channel space, just can construction sufficient amount first stop that projection 11, second stops that projection 12 and the 3rd stops projection 13 so that blood cell and separating plasma, and need to provide more blood volume could obtain smoothly sensing result.In addition, described first stops that projection 11, second stops that projection 12, the 3rd stops that shape, size and each interval that stops 11,12,13 of projections of projection 13 are all the microstructure with certain specification, therefore, need utilize etching mode just can make described first and stop that projection 11, second stops that projection 12 and the 3rd stops the structure of projection 13, so, processing procedure time lengthening not only, manufacturing cost also significantly increases, cause this sensing test piece 1 not good in the economic benefit of manufacturing on producing, industry applications is also relatively low.

In addition, as United States Patent (USP) the 6th, 966, No. 977 disclosed electrochemical sensing test pieces, mainly utilize filtrator to filter and treat fluid measured, and guide the blood plasma of filtering blood cell to enter the test solution supply passageway that disposes responding layer and electrode system, wherein, also add following restrictive condition: the sectional area of this filter inlet side is greater than the sectional area of its outgoing side, and this test solution supply passageway and be all less than the sectional area of this filter inlet side with the sectional area of the adjacent peristome of this filtrator.By the sectional area of the tip part (outgoing side) of contiguous this test solution supply passageway of filtrator is diminished, make blood plasma can more promptly flow to this filtrator tip and enter this test solution supply passageway.Yet the electrochemical sensing test piece of this kind of pattern in fact still has following shortcoming:

One, when this filtrator and this test solution supply passageway and this electrode system be horizontal side to configuration pattern time, when arranging, the outgoing side of this filtrator can not extend through the peristome of this test solution supply passageway, otherwise can touch electrode system and have influence on detecting result, and this kind of configuration mode enable to make blood plasma more promptly arrive at this filtrator tip by the sectional area version of this filtrator, but being accumulated to is enough to cause the detecting amount of effective response and still wants the long period, makes this sensing test piece still need the time more of a specified duration could obtain detecting result.

Two, the test solution supply passageway due to this sensing test piece is to coordinate this responding layer and electrode system to be designed to predetermined bulk, and its size is difficult for changing, in order to meet the sectional area of the on the suction side of this filtrator, be greater than the design requirement of this test solution supply passageway, the size that certainly will will relatively widen or thicken this filtrator, so may cause this filtrator overall volume to become large, to improve on the contrary and treat that the amount of fluid measured could obtain detecting result smoothly, equally also can extend the detecting time.

For another example United States Patent (USP) the 5th, 628, No. 890 disclosed electrochemical sensing test pieces, its opening for splashing into for fluid to be measured, filtering layer and electrode unit are presented lower vertical configuration, in response to this kind of arranged perpendicular structure, this filtering layer can cover all electrodes (being three electrodes in embodiment), although this kind of structure setting seems, can shorten the distance between the blood place of splashing into be measured and electrode unit and likely obtain more quickly measurement result, but for the red blood cell in certain filtering blood, still must increase the electric insulation layer of the number of plies of filtering layer the several layers of hydrophobicity material of arranging in pairs or groups and form sandwich construction, and this kind of sandwich construction needs relatively large blood flow volume could obtain smoothly measurement result, and the micropump or exert pressure of conventionally need to arranging in pairs or groups, otherwise easily there is the inaccurate situation of detecting result, because the composite unit number of packages that the electrochemical sensing chip of this kind of structure is required is more, necessary collocation pressure apparatus, and need provide more test blood volume, and still deposit space to be improved.

More than explanation shows in order to promote detecting accuracy the sensing test piece of different types with promoting detecting speed, but in order to provide result of use better product, still has the demand of the sensing test piece that continues exploitation different designs pattern.

Summary of the invention

The object of the present invention is to provide a kind of relatively easily manufacture, and contribute to obtain accurate sensing result fast, and there is the preferably electrochemical sensing chip of quantitative detection performance.

Electrochemical sensing chip of the present invention, be applicable to detect a target analytes for the treatment of in fluid measured, this sensing test piece comprises a housing unit, is separately positioned on an electrode unit in this housing unit, a reagent reacting layer contacting with this electrode unit, and one covers in this reagent reacting layer of part and the filtering element of the electrode unit of part.

This housing unit comprises an insulated substrate and a cover plate of corresponding joint, and this insulated substrate matches with cover plate and defines one and be installed in space, a peristome, and one be connected to this and be installed in the guiding channel between space and this peristome, this insulated substrate has respectively towards this cover plate one and coordinates the extension groove face, one that form this guiding channel to be adjacent to this extensions groove face and to coordinate formation that this is installed in the inner groove faces in space, and several perforation that is formed at intervals this inner groove faces.

This electrode unit is separately positioned in the perforation of insulated substrate of this housing unit, and has at least one lower than the sagging end face of this inner groove faces.

This reagent reacting layer is arranged on being installed in space and with this electrode unit of this housing unit and contacts, comprise can with the aitiogenic sensing reagent of target analytes.

This filtering element is made by the material with pore texture, and the peristome of this housing unit is extended laterally and is entered this and be installed in space by this guiding channel certainly, and hide on the sagging end face of the reagent reacting layer of part and the electrode unit of part, this filtering element has a wide path that is positioned at the peristome of this housing unit, one and is positioned at the narrow footpath section that this is installed in space, and one be connected to this wide path and narrow footpath is intersegmental and certainly this wide path to this narrow footpath section, be the linkage section of taper in width.

Preferably, the insulated substrate of this housing unit has two perforations separately, this electrode unit comprises first electrode plugging respectively in described perforation, an and working electrode, the sagging end face of this electrode unit is formed on one of them electrode of this first electrode and this working electrode, and this first electrode is selected from electrode, reference electrode, and the described combination to electrode and described reference electrode.

Preferably, the sagging end face of this electrode unit is formed at this first electrode, and this filtering element covers in the sagging end face of part reagent reacting layer and this first electrode.

Preferably, the sagging end face of this electrode unit also can be formed at this working electrode, and this filtering element covers in the sagging end face of part reagent reacting layer and this working electrode.

Preferably, this electrode unit also can have two sagging end faces, and be formed at respectively this first electrode and this working electrode, and this filtering element covers in part reagent reacting layer, and cover in the sagging end face of one of them electrode of this first electrode and this working electrode.

Preferably, this filtering element is del pattern, and this narrow footpath section ends at an end edge.

Preferably, the end edge of the narrow footpath section of this filtering element is arc profile through leading fillet to modify.

Preferably, the extension groove face of this insulated substrate is that shape that should filtering element is to gradually narrow design type of conduit from peristome to inner groove faces, and can be accommodating for this filtering element engaging.

Preferably, the extension groove face of the insulated substrate of this housing unit is the staircase structure of upwards climbing gradually to this inner groove faces from this peristome, and this filtering element along this extension groove face setting and be folded in this insulated substrate and this cover plate between.

Preferably, this housing unit also comprises that one is installed in this suction inlet that space is connected, and this sensing test piece also comprises one to being arranged on this housing unit by suction inlet, for attracting the fluid measured for the treatment of on this filtering element to be installed in towards this suction device advancing in space.

Preferably, the cover plate of this housing unit also has a pore running through being installed in space.

Preferably, the hole aperture in the pore texture of this filtering element is 0.8 μ m～8 μ m.

Preferably, the cover plate of this housing unit has one to should peristome setting and be installed in towards this circular arc cutaway that space is arranged with, and the wide path of this filtering element has one to should circular arc cutaway and be exposed sample and enter district.

Preferably, the extension groove face of this insulated substrate and the axial clearance between this cover plate are installed in space from this peristome to this and dwindle gradually, make this filtering element more approach this and are installed in space and bear larger squeezing action power.

Preferably, this filtering element increases gradually from peristome to the density that this is installed in space of this housing unit, and the average pore size of its pore texture is dwindled gradually.

Preferably, this electrochemical sensing chip can be applicable to a product being selected from following group: the sensor of specific biochemical component in family expenses medical treatment detecting device, biological sensor and test fluid.

Beneficial effect of the present invention is: the form that arranges in space that is installed in that extends into this housing unit by this filtering element is, it is comparatively contiguous with this electrode unit when energy accelerates to guide the target analytes in fluid measured to arrive this reagent reacting layer, whereby, the required reaction time can be shortened, and can obtain more quickly detecting result, make the present invention that preferably sensing efficiency can be provided.

Accompanying drawing explanation

Fig. 1 is a schematic top plan view, and United States Patent (USP) 6,319 is described, forms several half-moon-shaped situations that stop projection in a sensing test piece in No. 719 cases;

Fig. 2 is a perspective exploded view that does not contain reagent reacting layer, and the configuration scenario of a housing unit of the first preferred embodiment of electrochemical sensing chip of the present invention, an electrode unit and a filtering element is described;

Fig. 3 is a schematic top plan view, and the situation that this first preferred embodiment has been combined is described;

Fig. 4 is a cross-sectional schematic of getting along the straight line IV-IV in Fig. 3, and filtering element, electrode unit and the configuration scenario of one deck reagent reacting layer in this housing unit of this first preferred embodiment is described;

Fig. 5 is a cross-sectional schematic, illustrates that the second preferred embodiment of electrochemical sensing chip of the present invention is provided with the situation of a suction device;

Fig. 6 is a schematic top plan view, and the pattern of different designs of an insulated substrate of this housing unit is described;

Fig. 7 is a cut-open view taking from the line VII-VII in Fig. 6;

Fig. 8 is a schematic top plan view, and the pattern of another kind design of the insulated substrate of this housing unit is described;

Fig. 9 is a cut-open view taking from line IX-IX of Fig. 8.

Embodiment

Below in conjunction with drawings and Examples, the present invention is described in detail.

Before the present invention is described in detail, be noted that in the following description content, similarly element represents with identical numbering.

Consult Fig. 2, Fig. 3 and Fig. 4, first preferred embodiment of electrochemical sensing chip 2 of the present invention is applicable to detecting and treats a target analytes 101 in fluid measured 10, this sensing test piece 2 comprises a housing unit 3, be arranged on an electrode unit in this housing unit 34, a reagent reacting layer 5 contacting with this electrode unit 4, and one covers in the reagent reacting layer 5 of part and the filtering element 6 of the electrode unit 4 of part.In the present embodiment, to take blood glucose meter this sensing test piece 2 to be described as example, that is to say that this treats that fluid measured 10 is for whole blood, this target analytes 101 is blood sugar, blood cell in whole blood is interfering material 102, but should not limit with this pattern of this sensing test piece 2, can be by changing the composition of this reagent reacting layer 5, make this sensing test piece 2 except for detecting the blood sugar of blood, also can be used for detecting uric acid, heavy metal in cholesterol concentration and sewage, the concentration of agricultural chemicals and poisonous chemical composition etc., whereby, make this sensing test piece 2 can further make biological sensor, the sensor of specific biochemical component in test fluid, and the various electrochemical sensing device product such as the medical detecting device of family expenses.

This housing unit 3 comprises an insulated substrate 31 and a cover plate 32 of corresponding joint, this insulated substrate 31 matches with cover plate 32 and defines one and be installed in space 301, a peristome 302, and one is connected to the guiding channel 303 that this is installed in 302 of space 301 and this peristomes.

This insulated substrate 31 has respectively towards this cover plate 32 one and coordinates the extension groove face 311, one that form this guiding channel 303 to be adjacent to this extensions groove face 311 and to coordinate formation that this is installed in the inner groove faces 312 in space 301, and several perforation 315 that is formed at intervals this inner groove faces 312.Wherein, this extension groove face 311 is the staircase structure of upwards climbing gradually to this inner groove faces 312 from this peristome 302, in the present embodiment, this extension groove face 311 has the first rank portion 313 of this peristome 302 of vicinity, and the second-order portion 314 of this inner groove faces 312 of vicinity, and the height of this second-order portion 314 is higher than the height of this first rank portion 313.Wherein, this first rank portion 313, second-order portion 314 are all the obtuse angle structures that angle is greater than 90 °.In addition, the quantity of rank portion can increase and decrease according to actual demand.

This cover plate 32 has one to should peristome 302 arranging and be installed in space 301 towards this and be arranged with and make circular arc cutaway that part filtering element 6 exposes 321, one should guiding channel 303 extend to this sight that is installed in space 301 from 321 pairs of this circular arc cutaway to look conduit 322, and one to being installed in the pore 323 running through in space 301.This pore 323 is mainly for causing capillary action, makes this treat that fluid measured 10 can successfully advance to this along this guiding channel 303 and be installed in space 301 in this filtering element 6.

Preferably, this housing unit 3 also comprises that covers the observation luffer boards 33 that conduit 322 is looked in this sight, user can observe luffer boards 33 see by this and look the filtering element 6 arranging along this guiding channel 303, and then can when detect, see and depending on this, treat the mobile situation of fluid measured 10 on filtering element 6.

This electrode unit 4, its electrode is separately positioned in the perforation 315 of insulated substrate 31 of this housing unit 3, and has at least one lower than the sagging end face 401 of this inner groove faces 312.

In this present embodiment, this insulated substrate 31 has two perforations 315 separately, and this electrode unit 4 comprises being and does not come off and plug respectively first electrode 41 in described perforation 315, an and working electrode (wording electrode) 42, 4 of this electrode units have two sagging end faces 401 that are formed at respectively on this first electrode 41 and this working electrode 42, this first electrode 41 is selected from electrode (counter electrode), reference electrode (reference electrode), and the combination of described electrode.At the present embodiment the first electrode 41 used, be by electrode is combined with reference electrode, form and there is bipolar electrode function, and contiguous this of this first electrode 41 extends groove face 311, this working electrode 42 extends groove face 311 away from this, but this first electrode 41 still can reach identical use function with working electrode 42 transpositions.After setting completes, make the described sagging end face 401 of this first electrode 41 and this working electrode 42 all lower than this inner groove faces 312, whereby, this first electrode 41 matches with described perforation 315 respectively and forms two sinking space 316 with the sagging end face 401 of working electrode 42.Wherein, the electrode number of this electrode unit 4 is not limited, can be because of the electrode of the different different numbers of arranging in pairs or groups of electrochemical action demand during practical application, therefore, this electrode unit 4 can be also comprise working electrode, to electrode and reference electrode and form three-electrode system, or, can be also to comprise working electrode, the multi-electrode system to electrode, reference electrode and at least one detecting electrode (detecting electrode).In addition, also can be only at this first electrode 41, or only at this working electrode 42, form the end face 401 that sink, and when configuration, make to be formed with contiguous this extension groove face 311 of electrode of end face 401 of sinking, still can reach predetermined effect.

This reagent reacting layer 5 is arranged on being installed in space 301 and contacting with this electrode unit 4 of this housing unit 3, comprise can with the aitiogenic sensing reagent 51 of target analytes 101.Wherein, this reagent reacting layer 5, except along these inner groove faces 312 coatings are laid, is also filled in the sinking space 316 on this first electrode 41 and working electrode 42 end faces.

This filtering element 6 is to be made by the material with pore texture, and the hole aperture in its pore texture is 0.8 μ m～8 μ m.In addition, this filtering element 6 is to be extended laterally and entered this and be installed in space 301 by this guiding channel 303 from the peristome 302 of this housing unit 3, and hides on the sagging end face 401 of the reagent reacting layer 5 of part and the electrode unit 4 of part.Because the electrode unit 4 of the present embodiment is all formed with the end face 401 that sink on this first electrode 41 and this working electrode 42, therefore, this filtering element 6 can only cover in the sagging end face 401 of one of them electrode according to this first electrode 41 and the configuration mode of working electrode 42, normally hide the sagging end face 401 of that electrode of contiguous this guiding channel 303.In addition,, if while only having one of them

electrode

41,42 to be formed with sagging end face 401, make this filtering element 6 hide that

electrode

41,42 that is formed with the end face 401 that sink.In the present embodiment, this filtering element 6 is to hide on this first electrode 41.In addition, if when this electrode unit 4 is three-electrode system, this filtering element 6 can only cover in one of them electrode, or two electrodes wherein, no matter be for bipolar electrode, three electrodes or multi-electrode system, while arranging, take and do not make this filtering element 6 cover in all electrodes completely as principle.

Preferably, this filtering element 6 has a wide path that can be placed in this peristome 302 61, one and can be placed in this and be installed in the narrow footpath section 62 in space 301, and one be connected to this

wide path

61 and 62 of narrow footpath sections and certainly this wide path 61 to narrow footpath section 62, be the linkage section 63 of taper in width, this wide path 61 has one to circular arc cutaway 321 that should cover plate 32 and is exposed sample and enters district 611.In the present embodiment, this filtering element 6 is width by large diminishing del diaphragm structure, and this narrow footpath section 62 ends at an end edge 621.Wherein, for the filter liquor that makes to contain target analytes 101 causes electrochemical reaction sooner to obtain fast sensing result, to make this filtering element 6 extend into this to be installed in space 301, therefore, this end edge 621 is positioned at this reagent reacting layer 5 and these electrode unit 4 top (see figure 4)s, whereby, the filter liquor that makes to contain target analytes 101 enters this reagent reacting layer 5 and quite approaches this electrode unit 4, therefore can cause more quickly this electrode unit 4 and produce and reply.

What deserves to be explained is, although this filtering element 6 is to be the configuration mode hiding above this first electrode 41, but by this end edge 621, be the design of horn structure, still can make the filter liquor that contains target analytes 101 by centralized collection outflow fast, thereby can obtain fast sensing result.In addition, by forming this sagging end face 401 and then define this sinking space 316 at this first electrode 41, make this

filtering element

6 and 41, this first electrode there is larger spacing distance, even so this filtering element 6 extend to the position that hides above this first electrode 41 also unlikely generation filtering element 6 touch this first electrode 41, and interfere with the bad situation of testing result, thereby can successfully obtain sensing result accurately.

Preferably, shape due to this filtering element 6, make this treat that fluid measured 10 can produce gap at the tip of filtering element 6 and the flow velocity of two flanks, because the current difference distance of diverse location is excessive easily coated wherein in producing bubble, for fear of this bad situation, make this end edge 621 be arc profile through leading fillet to modify, just can reduce Bubble formation and then avoid detecting accuracy being affected.And this kind of structural design, do not affect the filter liquor that contains target analytes 101 via this filtering element 6 by centralized collection the effect that flows out fast, and make this reagent reacting layer 5 be easier to detect target analytes 101, and response result more accurately can be provided rapidly.

In addition, this filtering element 6 arranges along the extension groove face 311 of the insulated substrate 31 of this housing unit 3, and be folded in 32 of this insulated substrate 31 and this cover plates, in the present embodiment, to make the axial clearance of 32 of this extension groove face 311 and this cover plates to this, be installed in space 301 from this peristome 302 to dwindle gradually, making this filtering element 6 more approach this is installed in space 301 and bears larger squeezing action power, except making the sectional area of this filtering element 6 be installed in space 301 to this from this peristome 302, because being squeezed, be more diminishing change shape, the density of this filtering element 6 also can increase gradually, and the average pore size of its pore texture is also dwindled gradually from this wide path 61 to this end edge 621, cause the closer to the suffered barrier effect of this end edge 621 these interfering materials 102 larger, and contribute to make this interfering material 102 separated fast with target analytes 101.In addition, this extension groove face 311 is that shape that should filtering element 6 is to gradually narrow design type of conduit from this peristome 302 to this inner groove faces 312, and can be accommodating for these filtering element 6 engagings, whereby, can utilize this extension groove face 311 and the structural design that this filtering element 6 fastens to limit these filtering element 6 displacements and auxiliary positioning effect can be provided.

It should be added that, to select glass fibre material to make this filtering element 6 in the present embodiment, but should not limit with this material of this filtering element 6, also can use mixed fibre material to make this filtering element 6, and in the present embodiment in order to reach filtering interfering material (red blood cell) 102, and the effect that allows target analytes 101 pass through smoothly, be the particle diameter gap that coordinates interfering material 102 and target analytes 101, and the average pore size of the pore texture of this filtering element 6 is designed to 0.8 μ m～8 μ m.

During use, the sample that fluid measured 10 from top to bottom drops in the filtering element 6 exposing from the circular arc cutaway 321 of this cover plate 32 for the treatment of of scheduled volume is entered in district 611, make this treat that fluid measured 10 is adsorbed in this filtering element 6, then, by capillary action, along this filtering element 6, by this guiding channel 303, to this, being installed in space 301 again moves, because this treats that suffered absorption affinity and the capillary force in this filtering element 6 of target analytes 101 and interfering material 102 in fluid measured 10 is different, make the two difference because of translational speed reach the object being separated, whereby, make translational speed faster target analytes 101 first arrive at this reagent reacting layer 5, and can in interference-free situation, there is biochemical reaction with sensing reagent 51, again by these electrode unit 4 priming signals, and can obtain more accurate testing result.

In addition, utilize the extension groove face 311 of this insulated substrate 31, inner groove faces 312 matches with this cover plate 32, form this peristome 302 and treat that at this aperture area in working direction of fluid measured 10 is greater than the aperture area of this guiding channel 303, and the aperture area of this guiding channel 303 is greater than this Space that is installed in the aperture area in space 301, make equally this filtering element 6 because be subject to squeezing action power in various degree, and this peristome 302 is installed in space 301 to this to be the diminishing variation of sectional area more remarkable certainly, and contribute to make this to treat that the interfering material 102 in fluid measured 10 is separated fast with target analytes 101.

As shown in Figure 6, from vertical view, can find out the extension groove face 311 of this insulated substrate 31 and outer surface that should peristome 302 is partly to the trapezoidal of taper in width from outside to inside, this inner groove faces 312 is the fixing long finger-type of width, preferably, this insulated substrate 31 also has an obstruct at the blend stop 317 of a lateral projection of 311 of this inner groove faces 312 and this extension groove faces, as shown in Figure 7, for taking from the cut-open view of line VII-VII of Fig. 6, it shows that this extension groove face 311 is the inclined-plane form rising gradually from this peristome 302 to this blend stop 317, whereby, when this cover plate of collocation, 32(is shown in Fig. 2) time, will present the closer to the diminishing state of the formed aperture area of this inner groove faces 312, and then see Fig. 2 to being clamped in this cover plate 32() and the filtering element 6(of 31 of this insulated substrates see Fig. 2) produce squeezing effect in various degree.In addition, at these inner groove faces 312 coating reaction reagents, to form reagent reacting layer 5(, see Fig. 4) time, can utilize this blend stop 317 to prevent that reaction reagent from spilling and flowing to this peristome 302 from this inner groove faces 312, to complete smoothly the coating of reaction reagent.

As shown in Figure 8, situation for the another kind of design type of this insulated substrate 31, from vertical view, can find out this extension groove face 311 and outer surface partial design that should peristome 302 be become to the circular groove pattern of sealing, and this inner groove faces 312 is the fixing long finger-type of width equally, this insulated substrate 31 has equally an outer peripheral edges setting along this circular groove and intercepts the blend stop 317 in a lateral projection of 311 of this inner groove faces 312 and this extension groove faces, as shown in Figure 9, for taking from the cut-open view of the line IX-IX in Fig. 8, be presented at this extension groove face 311 and define a slot space of formation, and there is a trench bottom 318 and the upwardly extending groove sidepiece 319 of this trench bottom 318 certainly, and the adjacent part of this groove sidepiece 319 and this blend stop 317 is to be the inclined-plane form rising gradually to this blend stop 317 from this trench bottom 318, whereby, when this cover plate of collocation, 32(is shown in Fig. 2) time, the insulated substrate 31 of this kind of pattern can present equally the closer to the diminishing state of the formed aperture area of this inner groove faces 312.In addition, can prevent that the reaction reagent of coating from spilling from this inner groove faces 312 by this blend stop 317 equally, and can complete smoothly the coating of reaction reagent.

Consult Fig. 5, one second preferred embodiment for electrochemical sensing chip of the present invention, the main difference of this second preferred embodiment and this first preferred embodiment for this sensing test piece 2 also comprise one to should filtering element 6 suction devices 7 that arrange, and the suction effect for fear of this suction device 7 weakens, this cover plate 32 is not provided with this pore 323(and sees Fig. 4), remainder is identical with this first preferred embodiment, repeats no more.

Coordinate the installation of this suction device 7, this housing unit 3 also comprises that one is installed in this suction inlet 304 that space 301 is connected, and 7 pairs of this suction devices should be arranged on this housing unit 3 by suction inlet 304, for attracting, on this filtering element 6, treat that fluid measured 10 is installed in space 301 towards this and advances, the translational speed for the treatment of fluid measured 10 to accelerate this, and then the auxiliary filtration efficiency of promoting.In the present embodiment, this suction inlet 304 is formed at this insulated substrate 31, and this suction device 7 is installed in the interior formation suction in space 301 by this suction inlet 304 at this, to attract this to treat that fluid measured 10 is installed in space 301 towards this and moves.Wherein, this suction device 7 is a micropump.

Conclude above-mentionedly, electrochemical sensing chip 2 of the present invention, can obtain following effect and advantage, therefore can reach object of the present invention:

One, electrochemical sensing chip 2 of the present invention, belong to a kind of side direction configuration structure, the sample of namely treating fluid measured 10 on this filtering element 6 for receiving enters district 611 and the pattern of electrode unit 4 for the configuration that is staggered along a horizontal direction, whereby, only need just can reach by the capillary action of this filtering element 6 effect of separate targets analyte 101 and interfering material 102, and do not need as United States Patent (USP) the 5th, 628, multilayer overlayer is set for No. 890 and treating the fluid measured smooth filtering interfering material 102 of 10 ability and obtaining testing result of more amount will be provided, the present invention enters the form that arranges in space 301 that is installed in of this housing unit 3 again by this filtering element 6 is extended laterally, in the time of just further accelerating to guide this filter liquor that contains target analytes 101 in fluid measured 10 arrive and enter this reagent reacting layer 5, quite near this electrode unit 4, whereby, the required reaction time can be shortened, and can obtain more quickly detecting result.

Two, the architectural characteristic that is del diaphragm by this filtering element 6, make this filtering element 6 compared to existing rectangular filter membrane, can reduce approximately half total adsorption area and volume, and can effectively reduce required fluid measured 10 consumptions for the treatment of, and be that use amount reduces, by this filtering element 6, at this, treat that fluid measured 10 working direction are gradually narrow design of width, can impel target analytes 101 to be intensively gathered in gradually this end edge 621, and enter

reagent reacting layer

5 and 51 effects of sensing reagent, and then cause relevant biochemical reaction and reply, whereby, the present invention can be reduced and treat the consumption of fluid measured 10 and still can obtain testing result accurately.

Three, by being extended to, this filtering element 6 covers in the design of part reagent reacting layer 5 and partial electrode unit 4, make this

filtering element

6 and 5, this reagent reacting layer have larger overlapping area, and make the target analytes 101 of the second-order portion 314 by this extension groove face 311 can enter in large quantities this reagent reacting layer 5 within a short period of time, and this treats that interfering material 102 in fluid measured 10 also can more be difficult for arriving at unlikely testing result is produced of this second-order portion 314 and disturb because of the double action of gravity and absorption affinity, whereby, make this target analytes 101 there are biochemical reactions and produce to reply in the while at short notice with the sensing reagent 51 in this reagent reacting layer 5, can promote sensing efficiency equally.

Four, this housing unit 3 is dwindled gradually through this guiding channel 303 to the aperture area that this is installed in space 301 from this peristome 302, can change density and the mean pore size of this filtering element 6, and make this treat that the target analytes 101 in fluid measured 10 is separated more fast with interfering material 102, contribute to equally to improve sensing efficiency.

Five, with these filtering element 6 these suction devices 7 of collocation, can further accelerate this target analytes 101 translational speeds, make the present invention obtain testing result fast and there is preferably sensing efficiency, thereby can meet functional need fast and accurately.

Six, by the staircase structure face pattern that the extension groove face 311 of this insulated substrate 31 is designed to upwards climb gradually, make this filtering element 6 along the surface structure of this extension groove face 311 in this guiding channel 303 along with treating that the working direction of fluid measured 10 forms by the upwardly extending state that has a down dip, whereby, can further expand this target analytes 101 can be separated fast with the translational speed drop of interfering material 102, and make weight and the relatively large interfering material 102 of volume more be difficult for entering this reagent reacting layer 5, when the present invention is detected, be more difficult for being interfered and contribute to obtain testing result more accurately.

Claims

1. an electrochemical sensing chip, be applicable to detect a concentration for the treatment of a target analytes in fluid measured, this sensing test piece comprises a housing unit, is separately positioned on an electrode unit in this housing unit, a reagent reacting layer and a filtering element, it is characterized in that:

This housing unit comprises an insulated substrate and a cover plate of corresponding joint, and this insulated substrate matches with cover plate and defines one and be installed in space, a peristome, and one be connected to this and be installed in the guiding channel between space and this peristome, this insulated substrate has respectively towards this cover plate one and coordinates the extension groove face, one that form this guiding channel to be adjacent to this extensions groove face and to coordinate formation that this is installed in the inner groove faces in space, and several perforation that is formed at intervals this inner groove faces;

This electrode unit is separately positioned in the perforation of insulated substrate of this housing unit, and has at least one lower than the sagging end face of this inner groove faces;

This reagent reacting layer is arranged on being installed in space and with this electrode unit of this housing unit and contacts, and comprises and the aitiogenic sensing reagent of target analytes;

This filtering element is made by the material with pore texture, and the peristome of this housing unit is extended laterally and is entered this and be installed in space by this guiding channel certainly, and hide on the sagging end face of the reagent reacting layer of part and the electrode unit of part, this filtering element has a wide path that is positioned at the peristome of this housing unit, one and is positioned at the narrow footpath section that this is installed in space, and one is connected to this wide path and narrow footpath is intersegmental and this wide path to narrow footpath section is taper in width certainly linkage section.

2. electrochemical sensing chip according to claim 1, it is characterized in that: the insulated substrate of this housing unit has two perforations separately, this electrode unit comprises first electrode plugging respectively in described perforation, an and working electrode, the sagging end face of this electrode unit is formed on one of them electrode of this first electrode and this working electrode, and this first electrode is selected from electrode, reference electrode, and the described combination to electrode and described reference electrode.

3. electrochemical sensing chip according to claim 2, it is characterized in that: the sagging end face of this electrode unit is formed at this first electrode, and this filtering element covers in the sagging end face of part reagent reacting layer and this first electrode.

4. electrochemical sensing chip according to claim 2, it is characterized in that: the sagging end face of this electrode unit is formed at this working electrode, and this filtering element covers in the sagging end face of part reagent reacting layer and this working electrode.

5. electrochemical sensing chip according to claim 2, it is characterized in that: this electrode unit has two sagging end faces, and be formed at respectively this first electrode and this working electrode, and this filtering element covers in part reagent reacting layer, and cover in the sagging end face of one of them electrode of this first electrode and this working electrode.

6. electrochemical sensing chip according to claim 1, is characterized in that: this filtering element is del pattern, and this narrow footpath section ends at an end edge.

7. electrochemical sensing chip according to claim 6, is characterized in that: the end edge of the narrow footpath section of this filtering element is arc profile through leading fillet to modify.

8. electrochemical sensing chip according to claim 1, is characterized in that: the extension groove face of this insulated substrate is that shape that should filtering element is to gradually narrow design type of conduit from peristome to inner groove faces, and can be accommodating for this filtering element engaging.

9. electrochemical sensing chip according to claim 1, it is characterized in that: the extension groove face of the insulated substrate of this housing unit is the staircase structure of upwards climbing gradually to this inner groove faces from this peristome, and this filtering element along this extension groove face setting and be folded in this insulated substrate and this cover plate between.

10. electrochemical sensing chip according to claim 1, it is characterized in that: this housing unit also comprises that one is installed in this suction inlet that space is connected, and this sensing test piece also comprises one to being arranged on this housing unit by suction inlet, for attracting the fluid measured for the treatment of on this filtering element to be installed in towards this suction device advancing in space.

11. electrochemical sensing chips according to claim 1, is characterized in that: the cover plate of this housing unit also has a pore running through being installed in space.

12. electrochemical sensing chips according to claim 1, is characterized in that: the hole aperture in the pore texture of this filtering element is 0.8 μ m～8 μ m.

13. electrochemical sensing chips according to claim 1, it is characterized in that: the cover plate of this housing unit has one to should peristome setting and be installed in towards this circular arc cutaway that space is arranged with, this filtering element has one to should circular arc cutaway and be exposed sample and enter district.

14. electrochemical sensing chips according to claim 1, it is characterized in that: the extension groove face of this insulated substrate and the axial clearance between this cover plate are installed in space from this peristome to this and dwindle gradually, make this filtering element more approach this and be installed in space and bear larger squeezing action power.

15. electrochemical sensing chips according to claim 14, is characterized in that: this filtering element increases gradually from peristome to the density that this is installed in space of this housing unit, and the average pore size of its pore texture is dwindled gradually.

16. electrochemical sensing chips according to claim 1, is characterized in that: this filtering element increases gradually from peristome to the density that this is installed in space of this housing unit, and the average pore size of its pore texture is dwindled gradually.

17. electrochemical sensing chips according to claim 1, is characterized in that: this electrochemical sensing chip is applied to a product being selected from following group: the sensor of specific biochemical component in family expenses medical treatment detecting device, biological sensor and test fluid.