A kind of bamboo shoots proteolysis method
Technical field
The present invention relates to a kind of bamboo shoots proteolysis method, specifically relate to a kind of processing method of utilizing organic acid hydrolysis bamboo shoots albumen and hydrolyzate.
Background technology
Bamboo shoots are taken as " treasure in the dish " from ancient times in China, nutritionally, the past has many people to think, though the bamboo shoots taste is delicious, does not have what nutrition, have in addition think " eat a meal bamboo shoot and will scrape three days oil ".In fact containing nutriments such as abundant protein, dietary fiber in the bamboo shoots, is the healthy food of the high fine low fat of a kind of high protein.How rich in protein takes full advantage of the key that becomes exploitation bamboo shoots product.
Protein forms a polypeptide chain by a-amino acid combination in certain sequence, again by the macromolecular compound of one or more than one polypeptide chains according to its ad hoc fashion be combined into.The protein of eating is hydrolyzed into after amino acid is absorbed through digestion in vivo, again the synthesized human desired protein.Comparatively speaking, amino acid is easier is absorbed by the body.Bamboo shoots albumen is made up of several amino acids, and wherein essential amino acid is nearly 7 kinds, and content is abundant.Thereby become amino acid or small peptide then can increase its nutrition the bamboo shoots proteolysis.
The hydrolysis method of protein mainly contains enzymatic isolation method, fermentation method and acid-hydrolysis method etc.
Enzymatic isolation method is by the catalysis under suitable condition of specific enzyme, makes the protein peptide bond fission, forms small peptide and amino acid.Enzymatic isolation method has following characteristics:
1, reaction temperature and, fast.
2, specificity is strong, the percent hydrolysis height.
3, severe reaction conditions, the easy deactivation of enzyme causes can't enzymolysis.
4, can not promote the stripping of mineral element in the raw material.
The fermentation rule is by microbe inoculation, utilizes microorganism to make proteolysis.Fermentation method has following characteristics:
1, reaction temperature and.
2, severe reaction conditions, condition change can suppress microbial growth, and reaction is slack-off maybe can't react and make.
3, the reaction time long, be unfavorable for control.
Compare with enzyme process and fermentation method, acid-hydrolysis method has the following advantages:
1, reaction condition is controlled, and industrial production is easy to reach.
2, can promote the stripping of mineral element in the raw material, strengthen nutrition.
3, percent hydrolysis height.
Though adopt organic acid hydrolysis hydrolysis rate and percent hydrolysis not as good as inorganic acid in the acid hydrolysis, still have very remarkable advantages:
1, organic acid corrosivity is low, and production safety requires low to reacting appliance.
2, organic acid can with raw material in mineral element form the material be easy to absorption of human body.
3, little, the accessory substance of environmental pollution can utilize or be easy to and remove.
Thereby how to utilize organic acid production and processing bamboo shoots protein product to become a major issue in the bamboo shoots deep processing.
Summary of the invention
The present invention is exactly in order to address the above problem, and selects suitable method production and processing bamboo shoots albumen for use.This method hydrolysis bamboo shoots albumen, craft science is controlled, and is easy to implement, and environmental pollution is little, amino acid content height in the hydrolysate, accessory substance can take full advantage of, and product is of high nutritive value.
The object of the invention also is to provide the processing method of residual acid in the hydrolysate, utilizes precipitation to remove or make its generation to have accessory substance and the reservation of nutritive value.
Concrete preparation technology of the present invention is as follows: bamboo shoots (bright bamboo shoot or dried bamboo shoot) are dried to water content at 0-15%, pulverize, (10 orders are following) sieves; The bamboo shoots powder is joined in the reactor, and add the organic acid soln that 1-10 doubly measures 0.5-10mol/L, in 80-120 ℃ hydrolysis 5-20 hour.Filter, filtrate is regulated the pH value to 3-7, filters again, and filtrate is concentrated into prescribed volume or concentrate drying, namely.
Can utilize ultrasonic in the hydrolytic process of the present invention or microwave auxiliary, improve percent hydrolysis and shorten hydrolysis time.
The present invention compared with prior art has following advantage:
1, the present invention makes the little macro-molecular protein of bamboo shoots solubility in water change into amino acid soluble in water and small peptide by the method for hydrolysis, so not only improved the recovery rate of bamboo shoots greatly, and the aqueous solution of gained is easy to be processed into the product of variform, and these extracts have more that nutrition is easier to be absorbed by the body simultaneously.
2, the organic acid hydrolysis of the present invention's employing, its technological parameter reaches easily in industrial production and controls.Acid resistance to reacting appliance is less demanding, and environmental pollution is little.Organic acid can promote the stripping of mineral element in the bamboo shoots, improves the nutritive value of product.
3, the used organic acid of the present invention, safety non-toxic.Residual organic acid adds the pH conditioning agent and can form nontoxic precipitation and removed, or generate can be water-soluble nutriment, as calcium lactate etc.After reactant liquor was regulated the pH value, the pH value was controlled the solubility that can increase mineral element in acidity, and remaining organic acid is retained in the reactant liquor as the tart flavour conditioning agent.
4, the used organic acid of the present invention and pH conditioning agent wide material sources are cheap and easy to get, are fit to big production and use.
The specific embodiment
The replenish the calcium preparation of bamboo shoots protein hydrolyzate of example 1
1, the preliminary treatment of bamboo shoots: get bamboo shoots (bright bamboo shoot are shelled), section, in 60 ℃ drying under reduced pressure to water content below 7%, pulverize, cross 40 mesh sieves, standby.
2, acid hydrolysis: take by weighing bamboo shoots dry powder 10kg, put in the retort, and add the lactic acid solution 40L of 3mol/L, insulation is 15 hours in 110 ℃, filters.
3, regulate pH value: it is an amount of to get calcium carbonate powder, joins in the hydrolyzate, and the limit edged stirs, and produce to there being bubble, and pH value is 4 o'clock, no longer adds calcium carbonate powder, placement 30min, and filtration is concentrated into prescribed volume.
4, allotment: get concentrate, add an amount of additive, can, sterilization is namely.
The reaction of lactic acid and calcium carbonate powder generates calcium lactate, and calcium lactate is a kind of replenishers of good calcium, and being everlasting replenishes the calcium adds in the product.Calcium lactate has certain solubility in water simultaneously, is rich in the reactant liquor outside each seed amino acid, the polypeptide, still contains abundant calcium lactate.
The preparation of example 2 lemon flavour bamboo shoots protein hydrolyzates
1, the preliminary treatment of bamboo shoots: get bamboo shoots (bright bamboo shoot are shelled), section, in 60 ℃ drying under reduced pressure to water content below 7%, pulverize, cross 40 mesh sieves, standby.
2, acid hydrolysis: take by weighing bamboo shoots dry powder 10kg, put in the retort, and add the citric acid solution 30L of 3mol/L, insulation is 20 hours in 115 ℃, filters.
3, regulate pH value: it is an amount of to get saturated limewater solution, joins in the hydrolyzate, mixes, and do not increasing to precipitation, and pH value is 4 o'clock, no longer adds calcium carbonate powder, placement 1h, and filtration, filtrate is concentrated into prescribed volume.
4, allotment: get concentrate and add an amount of additive, can, sterilization, namely.
Citric acid and limewash reaction generate calcium citrate, and calcium citrate is poor dissolution in water, forms precipitation, removes by filtration.The control of pH value is about 4, and then filtrate still contains a certain amount of citric acid.Thereby product is except being rich in amino acid, nutritious outside, mouthfeel acid is refreshing.
The preparation of example 3 compound sour bamboo shoots protein hydrolyzates
1, the preliminary treatment of bamboo shoots: get bamboo shoots (bright bamboo shoot are shelled), section, in 60 ℃ drying under reduced pressure to water content below 7%, pulverize, cross 40 mesh sieves, standby.
2, acid hydrolysis: take by weighing bamboo shoots dry powder 10kg, put in the retort, and add the lactic acid solution 20L of 3mol/L and the citric acid solution 10L of 3mol/L, mix, insulation is 20 hours in 115 ℃, filters.
3, regulate the pH value: it is an amount of to get calcium carbonate powder, joins in the hydrolyzate, and constantly being stirred to does not have bubble to produce, and the pH value is 4, no longer adds calcium carbonate powder, places 30min, filters, and filtrate is concentrated into prescribed volume.
4, allotment: get concentrate and add an amount of additive, can, sterilization, namely.
Lactic acid and citric acid can both make the bamboo shoots proteolysis, and remaining acid energy and calcium carbonate powder reaction, generate corresponding calcium salt, and a residual part is sour in hydrolyzate, thereby make hydrolyzate have unique local flavor.