CN102409412A - Method for preparing fibrilia by using alternaria tenuis DB3 strains - Google Patents
Method for preparing fibrilia by using alternaria tenuis DB3 strains Download PDFInfo
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- CN102409412A CN102409412A CN2011102533168A CN201110253316A CN102409412A CN 102409412 A CN102409412 A CN 102409412A CN 2011102533168 A CN2011102533168 A CN 2011102533168A CN 201110253316 A CN201110253316 A CN 201110253316A CN 102409412 A CN102409412 A CN 102409412A
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Abstract
The invention relates to a method for preparing fibrilia by using epicoccum nigrum DB3 strains. The method comprises the following steps: (1) preserving the epicoccum nigrum DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.
Description
Technical field
The invention belongs to fungi and prepare the flax fibre field, particularly a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, jute, bluish dogbane, bluish dogbane, sisal hemp, abaca, nettle and piemarker etc.Flax fibre is the general designation of the fiber obtained from various numb plants, has moisture absorption, ventilative, heat radiation and the incomparable advantage of other natural fabrics such as antibiotic.China is as one of the abundantest country of hemp resource in the world, and annual flax fibre of producing and goods thereof remove and satisfy the demand of domestic market, also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, earn foreign exchange nearly 10,000,000,000 dollars every year.At national energy-saving and emission-reduction policy and consumer's back to nature, pursue under green consumption idea promotes, bast-fibre green processing technology has become the research focus of field of textiles.
Flax, hemp, jute and bastose mainly consist of cellulose, also have non-cellulose materials such as more hemicellulose, pectin and lignin to be wrapped in the flax fibre surface in addition.Non-cellulose material gluings such as these colloids, particularly lignin make raw ramie be firm sheet strip in the fiber outside.So raw ramie must be removed colloids such as lignin through the process of coming unstuck together in spinning process, to satisfy spinning requirement.At present, bast fibre spinning industry kiering method commonly used mainly is a chemical method, promptly utilizes strong acid and strong base that raw ramie or numb rove are handled.The chemical treatment technology energy consumption is big, and the waste water of the big and discharging of equipment loss can't recycle, and pollution problem is serious.
Summary of the invention
Technical problem to be solved by this invention provides a kind of alternaric bacteria (Alternaria alternata) DB2 bacterial strain that utilizes and prepares the method for flax fibre, and this method technology is simple, is fit to large-scale industrial production.
A kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre of the present invention comprises:
(1) alternaric bacteria DB2 bacterial strain is stored in potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen buffer solution; Wherein frozen buffer solution is by potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and natrium citricum 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes; The prescription of potato dextrose agar is: potato 200g, glucose 20g, running water 1000mL, natural pH value;
(2) in the cooled 50mL potato glucose culture medium of sterilization, insert bacterium one ring of step (1) gained, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle and cultivate 48h;
(3) culture medium inoculated of step (2) gained is gone into flax lignin fermentation medium 5L, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle cultivation 48h and obtain zymocyte liquid; Wherein, flax fermentative medium formula is: flax raw ramie lignin 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100ml constant volume;
(4) the numb raw material after will sterilizing mixes by bath raio with step (3) gained zymocyte liquid at 1: 20, at 30 ℃, handles in the 160-220rpm shaking table 1～4 day, removes zymotic fluid, obtains flax fibre;
(5) above-mentioned flax fibre and degumming baths are mixed, under 70-100 ℃ of temperature, handled 1-8 hour, washing then, and by the method for routine oil, drying, the fiber of the usefulness that promptly obtains supplying weaving; Wherein flax fibre is 1 to the weight ratio of degumming baths: 8-1: 25, and the temperature of coming unstuck is 70-100 ℃, the time is 1-8 hour.
Numb raw material described in the step (4) is flax straw, linen thread and yarn, sodolin, hemp original stem, hemp yarn, hemp cloth, the former stem of jute, Jute thread and yarn, megila, the former stem of bluish dogbane, bluish dogbane yarn or bluish dogbane fabric.
Degumming baths described in the step (5) is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water; The weight ratio 1 of wherein peroxide degumming agent, chelating agent and hydrogen bond disrupting agent sum and the solution that comes unstuck: 8-1: 25.
Peroxide degumming agent described in the step (5) is hydrogen peroxide, potassium peroxydisulfate, sodium peroxydisulfate, ammonium persulfate, potassium percarbonate, SODIUM PERCARBONATE, percarbonic acid ammonium, potassium perborate, sodium perborate or ammonium pertorate, and its consumption is the 0.25-8% of numb raw material weight.
Chelating agent described in the step (5) is sodium phosphate, sodium phosphate trimer, one or more of sodium metaphosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, citric acid, disodium ethylene diamine tetraacetate, and its consumption is the 0.1-4% of numb raw material weight.
Hydrogen bond disrupting agent described in the step (5) is a urea, and its consumption is the 0.05-2% of numb raw material weight.
Flax fibre described in the step (5) and degumming baths are 1: 10 mixed with weight ratio, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is an ammonium persulfate, and its consumption is 3% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 0.05% of a numb raw material weight, and chelating agent is sodium phosphate trimer and disodium ethylene diamine tetraacetate, and its consumption is respectively 2% and 0.1% of numb raw material weight.
Flax fibre described in the step (5) and degumming baths are 1: 25 mixed with weight ratio, and the temperature of coming unstuck is 90 ℃, and the time is 1 hour; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is a potassium perborate, and its consumption is 5% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 1% of a numb raw material weight, and chelating agent is sodium hydrogen phosphate and sodium dihydrogen phosphate, and its consumption is 2% of numb raw material weight.
Flax fibre described in the step (5) and degumming baths are 1: 8 mixed with weight ratio, and the temperature of coming unstuck is 100 ℃, and the time is 8 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is a sodium peroxydisulfate, and its consumption is 8% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 2% of a numb raw material weight, and chelating agent is sodium metaphosphate, disodium ethylene diamine tetraacetate and sodium phosphate trimer, and its consumption is 2% of numb raw material weight.
Flax fibre described in the step (5) and degumming baths are 1: 15 mixed with weight ratio, and the temperature of coming unstuck is 90 ℃, and the time is 2 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is the percarbonic acid ammonium, and its consumption is 2% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 0.05% of a numb raw material weight, and chelating agent is sodium phosphate trimer and sodium phosphate, and its consumption is respectively 2% and 1% of numb raw material weight.
The preserving number of the alternaric bacteria that the present invention adopted (Alternaria alternata) DB2 bacterial strain is CGMCC No.4695; Preservation day is on March 17th, 2011; Suggestion classification called after (Alternaria alternata); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, the address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
According to 18sR dna sequencing Blast and cluster analysis result, this bacterium and alternaric bacteria similitude are 99%, and in conjunction with physiological and biochemical test result and microscopy analysis, naming this bacterial strain is alternaric bacteria (Alternaria alternata) DB2 bacterial strain.
The preparation method of a kind of alternaric bacteria DB2 bacterial strain that the present invention adopted comprises:
(1) sea grass of will rotting places the enriched medium room temperature to leave standstill in the ratio of 1g: 20ml to cultivate 30 days, and got the 0.1ml enriched medium then and coat in the isolation medium, and 30 ℃ leave standstill and cultivated 1～4 day, obtain wild type flax biological treatment dominant strain;
(2) with the inoculation that obtains in the step (1) in flax lignin nutrient medium, cultivate 72h for 28 ℃;
(3) from above-mentioned flax lignin nutrient medium, select bacterial strain, promptly get with lignin degrading ability.
Enrichment culture based formulas in the said step (1) is: flax powder 5g, running water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH
4)
2SO
45g, K
2HPO
41g, MgSO
40.5g, KCl 0.5g, FeSO
40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.
The component of flax lignin nutrient medium in the said step (2) comprises: flax lignin 2g, NaNO
33g, K
2HPO
40.5g, MgSO
41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator.
Under the condition of culture that makes up, this strain culturing condition is extensive, good heat resistance, and growth and breeding is fast, can effectively remove the colloids such as lignin in flax, hemp and the jute-kenaf fibres, and enzyme is alive stable, bacterium, the equal avirulence of enzyme.This bacterial strain and culture systems can directly be used for flax degumming.
The strain growth cycle that the present invention adopted is short, and bacterial classification is difficult for contaminated, and processing cost is low, the mild condition of coming unstuck, and contamination resistance is strong, and heat resistance is good, non-environmental-pollution, it is good to handle the back fiber quality; This bacterial strain has the ability to bast fiber lignin degradations such as flax, hemp, jute or bluish dogbanes, can be applied to the bast fiber surface modification.
After bacterial classification pre-treatment or the processing, practical environmentally friendly peroxide carries out inactivation treatment, to substitute traditional boiling water inactivation technology, with using water wisely; Numb sample after the strong oxidizing property of utilizing peroxide in the inactivation technology is simultaneously handled bacterial classification carries out refining to be handled with bleaching, with further optimization technology, energy-saving and emission-reduction.
Compared with prior art, the present invention is applied to the deactivation of bacterial classification, the refining and the bleaching process of flax fibre with environmentally friendly peroxide, and three operations are merged into an operation.Therefore the present invention has that technology is simple, production procedure short, is fit to characteristics such as large-scale industrial production, and under mass production conditions, to carry out before bast fiber surface modification and the weaving processing the biological pre-treatment of lignin removal significant for exploring penicillium purpurogenum.
Beneficial effect
(1) bacterial strain that the present invention adopted and culture systems through detecting high yield pectase and hemicellulase simultaneously, can directly be used for flax degumming, have the cycle of coming unstuck weak point; The fiber dispersion rate reaches 100%, and is respond well to flax degumming, and the efficient of coming unstuck reaches 95%, and bacterial classification is difficult for contaminated; Processing cost is low, and fiber quality is good, the mild condition of coming unstuck; Contamination resistance is strong, and heat resistance is good, advantages such as non-environmental-pollution;
(2) preparation method of the present invention simple, be fit to characteristics such as large-scale industrial production.Utilize this system to carry out flax degumming and have the usually time weak point, the linen fibre dispersion rate reaches 100%, and degumming rate reaches more than 90%, and the degummed ramie quality is good.
Description of drawings
Fig. 1 is bacterial strain microscope dyeing photo (oily mirror);
Fig. 2 is a potato potato culture bacterium colony photo;
Fig. 3 is a fungi processing procedure photo;
Fig. 4 is for handling back linen fibre photo.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
The screening of flax degumming bacterial classification obtains
(1) the rotten sea grass of sampling from marine site, Zhoushan, the East Sea; Earlier sea grass and enriched medium are left standstill cultivation 30 days in ratio room temperature in enriched medium of 1g: 20ml; Get the 0.1ml enriched medium then and coat isolation medium; 30 ℃ leave standstill cultivation 1～4 day, obtain wild type flax biological treatment dominant strain;
Wherein, the enrichment culture based formulas is: flax powder 5g, running water 200ml, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH
4)
2SO
45g, K
2HPO
41g, MgSO
40.5g, KCl 0.5g, FeSO
40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(2) with the inoculation that obtains in the step (1) in flax lignin nutrient medium, cultivate 72h for 28 ℃.Flax lignin nutrient medium, its component comprises: flax lignin 2g, NaNO
33g, K
2HPO
40.5g, MgSO
41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(3) from above-mentioned culture medium, select bacterial strain with lignin degrading ability.
Embodiment 2
The preparation of zymocyte liquid:
(1) alternaric bacteria DB2 bacterial strain is stored in potato dextrose agar, and 30 ℃ of 220rpm cultivate 48h, add frozen buffer solution; Frozen buffer solution is by potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and natrium citricum 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes;
(2) in the cooled 50mL potato glucose culture medium of sterilization, insert bacterium one ring of step (1) gained, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle and cultivate 48h;
(3) culture medium inoculated of step (2) gained is gone into flax lignin fermentation medium 5L, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle cultivation 48h and obtain zymocyte liquid; Wherein, flax fermentative medium formula is: flax raw ramie lignin 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100ml constant volume.
Embodiment 3
Flax degumming technology is following:
The 5g flax straw is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of flax straw and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed degumming baths, stops biological treatment.Flax straw after the biological treatment is carried out inactivation treatment; Its technology is: flax straw after the biological treatment and degumming baths were with 1: 10 mixed; And under 70 ℃ condition, handled 3 hours, wherein the consumption of peroxide ammonium persulfate is 3% of a flax straw weight, amount of urea is 0.05% of a flax straw weight; The consumption of sodium phosphate trimer is 2% of a flax straw weight, and the consumption of disodium ethylene diamine tetraacetate is 0.1% of a flax straw weight.Linen fibre is used in 2130 weaving can making after coming unstuck.
Embodiment 4
The hemp cloth biological treatment is following:
Hemp cloth after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of hemp cloth and embodiment 2, liquid amount is 100ml, at 35 ℃, comes unstuck respectively in the 200rpm shaking table 2 days; Usually time is then removed degumming baths, stops biological treatment.Hemp cloth after the biological treatment is carried out inactivation treatment; Its technology is: hemp cloth after the biological treatment and degumming baths were with 1: 25 mixed; And under 90 ℃ condition, handled 1 hour, wherein the consumption of peroxide potassium perborate is 5% of a hemp cloth weight, amount of urea is 1% of a hemp cloth weight; The consumption of sodium hydrogen phosphate is 2% of a hemp cloth weight, and the consumption of sodium dihydrogen phosphate is 2% of a hemp cloth weight.The weaving of preparation is soft with hemp cloth after coming unstuck, whiteness is high, prodding and itching feeling significantly reduces.
Embodiment 5
The jute roving boiling and bleaching process is following:
Jute thread and yarn after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of Jute thread and yarn and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 4 days; Usually time is then removed degumming baths, stops biological treatment.Jute thread and yarn after the biological treatment is carried out inactivation treatment; Its technology is: Jute thread and yarn after the biological treatment and degumming baths were with 1: 8 mixed; And under 100 ℃ condition, handled 8 hours; Wherein the consumption of peroxide sodium peroxydisulfate is 8% of a Jute thread and yarn weight, and amount of urea is 2% of a Jute thread and yarn weight, and the consumption of sodium metaphosphate is 2% of a Jute thread and yarn weight; The consumption of disodium ethylene diamine tetraacetate is 2% of a Jute thread and yarn weight, and the consumption of sodium phosphate trimer is 2% of a Jute thread and yarn weight.Jute thread and yarn is used in 760 weaving can making after coming unstuck.
Embodiment 6
The flax roving boiling and bleaching process is following:
Linen thread and yarn (flax roving or second hards) after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid liquid of linen thread and yarn and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed degumming baths, stops biological treatment.Linen thread and yarn after the biological treatment is carried out inactivation treatment; Its technology is: linen thread and yarn after the biological treatment and degumming baths were with 1: 15 mixed; And under 90 ℃ condition, handled 2 hours, wherein the consumption of peroxide percarbonic acid ammonium is 2% of a linen thread and yarn weight, amount of urea is 0.05% of a linen thread and yarn weight; The consumption of sodium phosphate trimer is 2% of a linen thread and yarn weight, and the consumption of sodium phosphate is 1% of a linen thread and yarn weight.Linen thread and yarn is used in 1530 weaving can making after coming unstuck.
Embodiment 7
The sodolin biological treatment is following:
Sodolin after the 5g sterilization is inserted the 250ml triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid zymocyte liquid of sodolin and embodiment 2, liquid amount is 100ml, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed degumming baths, stops biological treatment.Sodolin after the biological treatment is carried out inactivation treatment; Its technology is: sodolin after the biological treatment and degumming baths were with 1: 10 mixed; And under 85 ℃ condition, handled 1 hour; Wherein the consumption of peroxide potassium peroxydisulfate is 2.5% of a sodolin weight, and amount of urea is 1% of a sodolin weight, and the consumption of sodium phosphate is 3% of a sodolin weight; The consumption of disodium ethylene diamine tetraacetate is 1% of a sodolin weight, and the consumption of citric acid is 1% of a sodolin weight.After coming unstuck, sodolin whiteness, pliability are significantly improved, and prodding and itching feeling significantly lowers.
Claims (10)
1. method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre comprises:
(1) alternaric bacteria DB2 bacterial strain is stored in potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen buffer solution; Wherein frozen buffer solution is by potassium dihydrogen phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and natrium citricum 0.0588g, bitter salt 0.02645g, glycerine 10ml adds water and is settled to 100ml and makes; The prescription of potato dextrose agar is: potato 200g, glucose 20g, running water 1000mL, natural pH value;
(2) in the cooled 50mL potato glucose culture medium of sterilization, insert bacterium one ring of step (1) gained, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle and cultivate 48h;
(3) culture medium inoculated of step (2) gained is gone into flax lignin fermentation medium 5L, under rotating speed 220r/min, 30 ℃ of conditions of temperature, shake bottle cultivation 48h and obtain zymocyte liquid; Wherein, flax fermentative medium formula is: flax raw ramie lignin 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100ml constant volume;
(4) the numb raw material after will sterilizing mixes by bath raio with step (3) gained zymocyte liquid at 1: 20, at 30 ℃, handles in the 160-220rpm shaking table 1～4 day, removes zymotic fluid, obtains flax fibre;
(5) above-mentioned flax fibre and degumming baths are mixed, under 70-100 ℃ of temperature, handled 1-8 hour, washing then, and by the method for routine oil, drying, the fiber of the usefulness that promptly obtains supplying weaving; Wherein flax fibre is 1 to the weight ratio of degumming baths: 8-1: 25, and the temperature of coming unstuck is 70-100 ℃, the time is 1-8 hour.
2. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1 is characterized in that: the numb raw material described in the step (4) is flax straw, linen thread and yarn, sodolin, hemp original stem, hemp yarn, hemp cloth, the former stem of jute, Jute thread and yarn, megila, the former stem of bluish dogbane, bluish dogbane yarn or bluish dogbane fabric.
3. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1, it is characterized in that: the degumming baths described in the step (5) is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water; Wherein peroxide degumming agent, chelating agent and hydrogen bond disrupting agent sum are 1 with the weight ratio of the solution that comes unstuck: 8-1: 25.
4. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 3; It is characterized in that: the peroxide degumming agent described in the step (5) is hydrogen peroxide, potassium peroxydisulfate, sodium peroxydisulfate, ammonium persulfate, potassium percarbonate, SODIUM PERCARBONATE, percarbonic acid ammonium, potassium perborate, sodium perborate or ammonium pertorate, and its consumption is the 0.25-8% of numb raw material weight.
5. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 3; It is characterized in that: the chelating agent described in the step (5) is sodium phosphate, sodium phosphate trimer; One or more of sodium metaphosphate, sodium hydrogen phosphate, sodium dihydrogen phosphate, citric acid, disodium ethylene diamine tetraacetate, its consumption is the 0.1-4% of numb raw material weight.
6. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 3, it is characterized in that: the hydrogen bond disrupting agent described in the step (5) is a urea, its consumption is the 0.05-2% of numb raw material weight.
7. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1 is characterized in that: flax fibre described in the step (5) and degumming baths are 1: 10 mixed with weight ratio, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is an ammonium persulfate, and its consumption is 3% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 0.05% of a numb raw material weight, and chelating agent is sodium phosphate trimer and disodium ethylene diamine tetraacetate, and its consumption is respectively 2% and 0.1% of numb raw material weight.
8. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1 is characterized in that: flax fibre described in the step (5) and degumming baths are 1: 25 mixed with weight ratio, and the temperature of coming unstuck is 90 ℃, and the time is 1 hour; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is a potassium perborate, and its consumption is 5% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 1% of a numb raw material weight, and chelating agent is sodium hydrogen phosphate and sodium dihydrogen phosphate, and its consumption is 2% of numb raw material weight.
9. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1 is characterized in that: flax fibre described in the step (5) and degumming baths are 1: 8 mixed with weight ratio, and the temperature of coming unstuck is 100 ℃, and the time is 8 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is a sodium peroxydisulfate, and its consumption is 8% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 2% of a numb raw material weight, and chelating agent is sodium metaphosphate, disodium ethylene diamine tetraacetate and sodium phosphate trimer, and its consumption is 2% of numb raw material weight.
10. a kind of method of utilizing alternaric bacteria DB2 bacterial strain to prepare flax fibre according to claim 1 is characterized in that: flax fibre described in the step (5) and degumming baths are 1: 15 mixed with weight ratio, and the temperature of coming unstuck is 90 ℃, and the time is 2 hours; Wherein degumming baths is made up of peroxide degumming agent, chelating agent, hydrogen bond disrupting agent and water, and the peroxide degumming agent is the percarbonic acid ammonium, and its consumption is 2% of a numb raw material weight; The hydrogen bond disrupting agent is a urea, and its consumption is 0.05% of a numb raw material weight, and chelating agent is sodium phosphate trimer and sodium phosphate, and its consumption is respectively 2% and 1% of numb raw material weight.
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Cited By (2)
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| CN104674354A (en) * | 2015-02-15 | 2015-06-03 | 东华大学 | Method for preparing fibrilia by combination of candida tropicalis DK2 strains and hydraulic peroxide ultrasonic wave |
| CN105238700A (en) * | 2015-09-24 | 2016-01-13 | 东北农业大学 | Wild soybean endophytic fungus high-yield in oleanolic acid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1924120A (en) * | 2006-09-11 | 2007-03-07 | 东华大学 | Method of preparing fiber for weave by peroxide degumming method |
| CN102010836A (en) * | 2010-08-13 | 2011-04-13 | 东华大学 | Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1924120A (en) * | 2006-09-11 | 2007-03-07 | 东华大学 | Method of preparing fiber for weave by peroxide degumming method |
| CN102010836A (en) * | 2010-08-13 | 2011-04-13 | 东华大学 | Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| A. DE CAL等: "Population dynamics of Epicoccum nigrum, a biocontrol agent against brown rot in stone fruit", 《JOURNAL OF APPLIED MICROBIOLOGY》, vol. 106, no. 2, 31 December 2009 (2009-12-31), pages 592 - 605 * |
| 张志刚等: "细极链格孢菌蛋白激发子对棉主要性状及相关酶的影响", 《中国农业大学学报》, vol. 12, no. 5, 31 December 2007 (2007-12-31), pages 16 - 21 * |
| 李扬等: "黑附球菌在植物病害生物防治中的研究与应用进展", 《安徽农业科学》, vol. 38, no. 6, 31 December 2010 (2010-12-31), pages 2988 - 2990 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104674354A (en) * | 2015-02-15 | 2015-06-03 | 东华大学 | Method for preparing fibrilia by combination of candida tropicalis DK2 strains and hydraulic peroxide ultrasonic wave |
| CN104674354B (en) * | 2015-02-15 | 2016-09-07 | 东华大学 | A kind of candida tropicalis DK2 bacterial strain and the ultrasonic combined method preparing flaxen fiber of hydrogen peroxide |
| CN105238700A (en) * | 2015-09-24 | 2016-01-13 | 东北农业大学 | Wild soybean endophytic fungus high-yield in oleanolic acid |
| CN105238700B (en) * | 2015-09-24 | 2018-04-03 | 东北农业大学 | One plant height produces the wild soybean endogenetic fungus of oleanolic acid |
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