CN102408493A - Sedum spectabile polysaccharide preparation method, and product and application thereof - Google Patents
Sedum spectabile polysaccharide preparation method, and product and application thereof Download PDFInfo
- Publication number
- CN102408493A CN102408493A CN2011104587092A CN201110458709A CN102408493A CN 102408493 A CN102408493 A CN 102408493A CN 2011104587092 A CN2011104587092 A CN 2011104587092A CN 201110458709 A CN201110458709 A CN 201110458709A CN 102408493 A CN102408493 A CN 102408493A
- Authority
- CN
- China
- Prior art keywords
- sedum spectabile
- spectabile boreau
- equivalent
- boreau
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a Sedum spectabile polysaccharide preparation method which comprises pretreatment, coarse extraction, concentration,alcohol precipitation, purification and other steps. The preparation method is simple to operate, the quality of the product is controllable, and the extraction efficiency is high. The invention also discloses a Sedum spectabile polysaccharide prepared by the method and a preparation thereof. The Sedum spectabile polysaccharide and the preparation thereof can be used for clinically treating diabetes, and have the advantages of obvious blood sugar reducing effect and little side effect, thereby having wide application prospects.
Description
Technical field
The invention belongs to pharmacy field, be specifically related to the preparation method of Sedum spectabile Boreau polysaccharide, also relate to the scorpion polysaccharide and the preparation thereof of this method preparation, also relate to the application of scorpion polysaccharide in preparation treatment diabetes medicament.
Background technology
Sedum spectabile Boreau (
Girardinia suborbiculata C.J.Chen), call red leaves of pulse plants hair grass, fire fiber crops grass, for the Urticaceae Sedum spectabile Boreau belongs to annual or perennial herb, be born in sylvan life or the cloudy place of limes marginis of height above sea level 50-800m.Be distributed in areas such as China Shaanxi, Gansu, Hunan, Sichuan, its aboundresources.Sedum spectabile Boreau is of long duration in China as medication among the people, mainly is used for treating rheumatic arthralgia, traumatic hemorrhage, ulcer, limb fiber crops etc.Recent study finds that Sedum spectabile Boreau also has effects such as the clotting time of prolongation, anticoagulant, and the Sedum spectabile Boreau extract has effects such as anti-inflammatory, gout, analgesia preferably.But preparation method and activity research aspect report thereof about the Sedum spectabile Boreau polysaccharide are less, and the Sedum spectabile Boreau polysaccharide does not obtain fully, effectively utilizes.
Therefore, be badly in need of a kind of preparation method of Sedum spectabile Boreau polysaccharide, simple to operate, quality controllable, extraction efficiency is high.
Summary of the invention
In view of this, one of the object of the invention is to provide the preparation method of Sedum spectabile Boreau polysaccharide, and this preparation method is simple to operate, and is quality controllable, and extraction efficiency is high.
For realizing above-mentioned purpose, technical scheme is:
The preparation method of Sedum spectabile Boreau polysaccharide may further comprise the steps:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and medicinal powder adds the refluxing extraction device, and adding volume(tric)fraction is the ethanol of 60-95%, and refluxing extraction is filtered, and abandons filtrating, adds the ethanol that volume(tric)fraction is 60-95% again, and refluxing extraction is filtered, and collects filter residue, promptly gets the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added water, is under the 60-90 ℃ of condition in temperature, extracts, and filters, and collects filtrating, time extracting solution of winning; In the dregs of a decoction, adding water again, is under the 60-90 ℃ of condition in temperature, extracts, and filtrating is collected in filtration, gets extracting solution for the second time, merges filtrating twice, gets crude extract;
C. concentrate
Step b gained crude extract is concentrated, carries out centrifugally then, collect supernatant, liquid concentrator;
D. alcohol precipitation
With adding ethanol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 60-85%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively, and drying gets the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is water-soluble, add protein liquid removal, thorough mixing leaves standstill; Collect water, in aqueous phase liquid, add gac, constant-temperature shaking, centrifugal; Collect supernatant, in interception is the dialysis tubing of 5000-20000Da, dialyse, lyophilize, the Sedum spectabile Boreau polysaccharide; Said protein liquid removal is chloroform and propyl carbinol mixture, and the volume ratio of chloroform and propyl carbinol is 4:1.
Further, said step a is the ethanol of 70-95% for adding the volume(tric)fraction that is equivalent to Sedum spectabile Boreau weight 1-10 times, refluxing extraction; Filter, abandon filtrating, adding and being equivalent to Sedum spectabile Boreau weight 1-10 volume(tric)fraction doubly is the ethanol of 70-95%; Refluxing extraction is filtered, and abandons filtrating;
Said step b is equivalent to Sedum spectabile Boreau weight 5-30 water doubly for adding, and is under the 80-90 ℃ of condition in temperature, extracts 1-3 hour, and add again and be equivalent to Sedum spectabile Boreau weight 5-30 water doubly, be under the 60-90 ℃ of condition in temperature, extracted 1-3 hour, filter, collect filtrating;
Said step c is concentrated into 1-3 that liquid concentrator is equivalent to Sedum spectabile Boreau weight doubly; Said centrifugal for being not less than 3000 rev/mins at rotating speed, centrifugally being no less than 10 minutes;
Said steps d is 70-85% for adding absolute ethyl alcohol to ethanol volume(tric)fraction;
Said step e is equivalent to Sedum spectabile Boreau Crude polysaccharides weight 10-40 water doubly for adding, and adds and is equivalent to volume of water 1/6-1/4 protein liquid removal doubly, thorough mixing; In temperature is hold over night under 4 ℃ of conditions, collects water, and adding gac to quality of activated carbon mark is 1%-10%; In temperature is constant-temperature shaking at least 1 hour under 40 ℃ of conditions; Being not less than 3000 rev/mins, centrifugally being no less than 10 minutes at rotating speed, collect supernatant, is dialysis at least 48 hours in the dialysis tubing of 8000-15000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Further, said step a is 95% ethanol for adding the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau volumes, refluxing extraction 1-3 hour; Filter, abandon filtrating, add the volume(tric)fraction that is equivalent to 5 times of Sedum spectabile Boreau volumes again and be 95% ethanol; Refluxing extraction 1-3 hour, filter, abandon filtrating;
Said step b is under 85 ℃ of conditions for adding the water that is equivalent to 20 times of Sedum spectabile Boreau weight in temperature, extracts 1.5 hours, in the dregs of a decoction, adds the water that is equivalent to 20 times of Sedum spectabile Boreau volumes again, is under 85 ℃ of conditions in temperature, extracts 1.5 hours, filters, and collects filtrating;
Said step c is concentrated into liquid concentrator to be equivalent to 2 times of Sedum spectabile Boreau weight, is 3000 rev/mins, centrifugal 30 minutes at rotating speed;
Said steps d is 80% for adding absolute ethyl alcohol to ethanol final concentration volume(tric)fraction;
Said step e adds the protein liquid removal that is equivalent to 1/5 times of volume of water, thorough mixing again for adding the water that is equivalent to 30 times of Sedum spectabile Boreau Crude polysaccharides weight; In temperature is hold over night under 4 ℃ of conditions, collects water, and adding gac to quality of activated carbon mark is 1.5%; In temperature is constant-temperature shaking 1 hour under 40 ℃ of conditions, is 3000 rev/mins, centrifugal 10 minutes at rotating speed, collects supernatant; In interception is dialysis 48 hours in the dialysis tubing of 8000-15000Da, lyophilize, the Sedum spectabile Boreau polysaccharide.
Two of the object of the invention is to provide the Sedum spectabile Boreau that makes with aforesaid method polysaccharide, and its purity is high.
For realizing above-mentioned purpose, technical scheme is:
Utilize the Sedum spectabile Boreau polysaccharide of described preparing method's preparation.
Three of the object of the invention is to provide the preparation that makes with the Sedum spectabile Boreau polysaccharide, and the said preparation cost is low, is convenient to clinical application.
For realizing above-mentioned purpose, technical scheme is:
The preparation that contains said Sedum spectabile Boreau polysaccharide.
Further, said preparation is a granular preparation.
Four of the object of the invention is to provide the application of Sedum spectabile Boreau polysaccharide, and result of treatment is definite, instant effect.
For realizing above-mentioned purpose, technical scheme is:
The application in preparation treatment diabetes medicament of said Sedum spectabile Boreau polysaccharide or preparation.
Beneficial effect of the present invention is: the present invention the preparation method of Sedum spectabile Boreau polysaccharide is disclosed, it is simple to operate, and is quality controllable, extraction efficiency high (extraction efficiency is up to 6.2%); Sedum spectabile Boreau polysaccharide content high (the Sedum spectabile Boreau polysaccharide content is up to 94.8% in the extract) in the extract; The Sedum spectabile Boreau polysaccharide of preparation is used to treat mellitus; Has good blood sugar decreasing effect; Particularly take the Sedum spectabile Boreau polysaccharide of high dosage, blood sugar decreasing effect is remarkable, the about 6.1mmol/L of blood glucose value decline; The Sedum spectabile Boreau polysaccharide that makes can be processed the various preparations that are applicable to that clinical treatment needs, and for example granular preparation is easy to use, and result of treatment is good, instant effect.
Embodiment
Below will combine embodiment that the present invention is carried out detailed description.The experimental technique of unreceipted actual conditions in the preferred embodiment is usually according to normal condition.
Embodiment 1
The preparation method of Sedum spectabile Boreau polysaccharide, concrete steps are:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and fine powder adds the refluxing extraction device, adds the volume(tric)fraction that is equivalent to 5 times of Sedum spectabile Boreau weight and be 95% ethanol; Refluxing extraction 2 hours is filtered, and abandons filtrating; Add the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau weight again and be 95% ethanol, refluxing extraction 2 hours is filtered; Abandon filtrating, promptly get the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added the water that is equivalent to 20 times of Sedum spectabile Boreau weight, is under 85 ℃ of conditions in temperature, and extraction time is 1.5 hours; Filter, collect filtrating, in the dregs of a decoction, add the water that is equivalent to 20 times of Sedum spectabile Boreau weight again; In temperature is 85 ℃; Extracted 1.5 hours, and filtered, collect filtrating; Merge filtrating twice, get extracting solution;
C. concentrate
Step b gained extracting solution is concentrated into liquid concentrator is equivalent to 2 times of Sedum spectabile Boreau weight, carrying out at rotating speed then is 3000 rev/mins, centrifugal 30 minutes, collects supernatant, liquid concentrator;
D. alcohol precipitation
With adding absolute ethyl alcohol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 85%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively 3 times, and drying must the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is added the water that is equivalent to 30 times of Sedum spectabile Boreau Crude polysaccharides weight, add the protein liquid removal (chloroform and propyl carbinol volume ratio are 4:1) that is equivalent to 1/5 times of volume of water, thorough mixing; Solid-liquid separation is collected water, and adding gac to quality of activated carbon mark to aqueous phase is 1.5%; 40 ℃ of constant-temperature shaking 1 hour; At rotating speed is 3000 rev/mins, centrifugal 10 minutes, collects supernatant, is dialysis 48 hours in the dialysis tubing of 5000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Present embodiment Sedum spectabile Boreau extracting efficiency of polysaccharides is 6.2%, and the purity of Sedum spectabile Boreau polysaccharide is 81.3% in the extract.
Embodiment 2
The preparation method of Sedum spectabile Boreau polysaccharide, concrete steps are:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and fine powder adds the refluxing extraction device, adds the volume(tric)fraction that is equivalent to 10 times of Sedum spectabile Boreau weight and be 90% ethanol; Refluxing extraction 1 hour is filtered, and abandons filtrating; Add the volume(tric)fraction be equivalent to 10 times of Sedum spectabile Boreau weight again and be 90% ethanol, refluxing extraction 1 hour is filtered; Abandon filtrating, promptly get the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added the water that is equivalent to 5 times of Sedum spectabile Boreau weight, is under 90 ℃ of conditions in temperature, and extraction time is 3 hours; Filter, collect filtrating, in the dregs of a decoction, add the water that is equivalent to 30 times of pre-treatment Sedum spectabile Boreau weight again; In temperature is 90 ℃, extracts 1 hour, filters; Collect filtrating, merge filtrating twice, get extracting solution;
C. concentrate
Step b gained extracting solution is concentrated into liquid concentrator is equivalent to 3 times of Sedum spectabile Boreau weight, carrying out at rotating speed then is 4500 rev/mins, centrifugal 10 minutes, collects supernatant, liquid concentrator;
D. alcohol precipitation
With adding absolute ethyl alcohol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 80%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively 2 times, and drying must the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is added the water that is equivalent to 10 times of Sedum spectabile Boreau Crude polysaccharides weight, add the protein liquid removal (chloroform and propyl carbinol volume ratio are 4:1) that is equivalent to 1/4 times of volume of water, thorough mixing; Solid-liquid separation is collected water, and adding gac to quality of activated carbon mark to aqueous phase is 10%; 40 ℃ of constant-temperature shaking 1 hour; At rotating speed is 4500 rev/mins, centrifugal 25 minutes, collects supernatant, is dialysis 56 hours in the dialysis tubing of 8000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Present embodiment Sedum spectabile Boreau extracting efficiency of polysaccharides is 5.8%, and the purity of Sedum spectabile Boreau polysaccharide is 85.2% in the extract.
Embodiment 3
The preparation method of Sedum spectabile Boreau polysaccharide, concrete steps are:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and fine powder adds the refluxing extraction device, adds the volume(tric)fraction that is equivalent to 1 times of Sedum spectabile Boreau weight and be 85% ethanol; Refluxing extraction 3 hours is filtered, and abandons filtrating; Add the volume(tric)fraction be equivalent to 1 times of Sedum spectabile Boreau weight again and be 85% ethanol, refluxing extraction 3 hours is filtered; Abandon filtrating, promptly get the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added the water that is equivalent to 30 times of Sedum spectabile Boreau weight, is under 80 ℃ of conditions in temperature, and extraction time is 1 hour, filters; Collecting filtrating, in the dregs of a decoction, add the water that is equivalent to 5 times of Sedum spectabile Boreau weight again, is 90 ℃ in temperature; Extracted 3 hours, and filtered, collect filtrating; Merge filtrating twice, get extracting solution;
C. concentrate
Step b gained extracting solution is concentrated into liquid concentrator is equivalent to 1 times of Sedum spectabile Boreau weight, carrying out at rotating speed then is 6000 rev/mins, centrifugal 15 minutes, collects supernatant, liquid concentrator;
D. alcohol precipitation
With adding absolute ethyl alcohol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 75%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively 5 times, and drying must the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is added the water that is equivalent to 40 times of Sedum spectabile Boreau Crude polysaccharides weight, add the protein liquid removal (chloroform and propyl carbinol volume ratio are 4:1) that is equivalent to 1/6 times of volume of water, thorough mixing; Solid-liquid separation is collected water, and adding gac to quality of activated carbon mark to aqueous phase is 1%; 40 ℃ of constant-temperature shaking 5 hours; At rotating speed is 3000 rev/mins, centrifugal 45 minutes, collects supernatant, is dialysis 48 hours in the dialysis tubing of 10000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Present embodiment Sedum spectabile Boreau extracting efficiency of polysaccharides is 5.5%, and the purity of Sedum spectabile Boreau polysaccharide is 87.0% in the extract.
Embodiment 4
The preparation method of Sedum spectabile Boreau polysaccharide, concrete steps are:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and fine powder adds the refluxing extraction device, adds the volume(tric)fraction that is equivalent to 5 times of Sedum spectabile Boreau weight and be 70% ethanol; Refluxing extraction 1 hour is filtered, and abandons filtrating; Add the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau weight again and be 70% ethanol, refluxing extraction 1 hour is filtered; Abandon filtrating, promptly get the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added the water that is equivalent to 25 times of Sedum spectabile Boreau weight, is under 60 ℃ of conditions in temperature, and extraction time is 1.5 hours; Filter, collect filtrating, in the dregs of a decoction, add the water that is equivalent to 25 times of Sedum spectabile Boreau weight again; In temperature is 60 ℃; Extracted 1.5 hours, and filtered, collect filtrating; Merge filtrating twice, get extracting solution;
C. concentrate
Step b gained extracting solution is concentrated into liquid concentrator is equivalent to 2 times of Sedum spectabile Boreau weight, carrying out at rotating speed then is 3000 rev/mins, centrifugal 30 minutes, collects supernatant, liquid concentrator;
D. alcohol precipitation
With adding absolute ethyl alcohol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 70%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively 3 times, and drying must the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is added the water that is equivalent to 20 times of Sedum spectabile Boreau Crude polysaccharides weight, add the protein liquid removal (chloroform and propyl carbinol volume ratio are 4:1) that is equivalent to 1/5 times of volume of water, thorough mixing; Solid-liquid separation is collected water, and adding gac to quality of activated carbon mark to aqueous phase is 5%; 40 ℃ of constant-temperature shaking 1 hour; At rotating speed is 3000 rev/mins, centrifugal 10 minutes, collects supernatant, is dialysis 48 hours in the dialysis tubing of 15000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Present embodiment Sedum spectabile Boreau extracting efficiency of polysaccharides is 5.1%, and the purity of Sedum spectabile Boreau polysaccharide is 90.2% in the extract.
Embodiment 5
The preparation method of Sedum spectabile Boreau polysaccharide, concrete steps are:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and fine powder adds the refluxing extraction device, adds the volume(tric)fraction that is equivalent to 5 times of Sedum spectabile Boreau weight and be 60% ethanol; Refluxing extraction 2 hours is filtered, and abandons filtrating; Add the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau weight again and be 60% ethanol, refluxing extraction 2 hours is filtered; Abandon filtrating, promptly get the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added the water that is equivalent to 25 times of Sedum spectabile Boreau weight, is under 90 ℃ of conditions in temperature, and extraction time is 1.5 hours; Filter, collect filtrating, in the dregs of a decoction, add the water that is equivalent to 25 times of Sedum spectabile Boreau weight again; In temperature is 90 ℃; Extracted 1.5 hours, and filtered, collect filtrating; Merge filtrating twice, get extracting solution;
C. concentrate
Step b gained extracting solution is concentrated into liquid concentrator is equivalent to 2 times of Sedum spectabile Boreau weight, carrying out at rotating speed then is 3000 rev/mins, centrifugal 30 minutes, collects supernatant, liquid concentrator;
D. alcohol precipitation
With adding absolute ethyl alcohol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 60%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively 3 times, and drying must the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is added the water that is equivalent to 20 times of Sedum spectabile Boreau Crude polysaccharides weight, add the protein liquid removal (chloroform and propyl carbinol volume ratio are 4:1) that is equivalent to 1/5 times of volume of water, thorough mixing; Solid-liquid separation is collected water, and adding gac to quality of activated carbon mark to aqueous phase is 5%; 40 ℃ of constant-temperature shaking 1 hour; At rotating speed is 3000 rev/mins, centrifugal 10 minutes, collects supernatant, is dialysis 48 hours in the dialysis tubing of 20000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
Present embodiment Sedum spectabile Boreau extracting efficiency of polysaccharides is 4.6%, and the purity of Sedum spectabile Boreau polysaccharide is 94.8% in the extract.
Embodiment 6
The preparation of Sedum spectabile Boreau polyoses grain agent
Get 50 parts in 50 parts in 150 parts of 400 parts of 400 parts of Sedum spectabile Boreau polysaccharide, starch, cane sugar powders, dextrin and the water of embodiment 1 preparation; Mix; Crossing 14 mesh sieves and process wet granular, is under 80 ℃ of conditions dry 3-6 hour in temperature, promptly gets the agent of Sedum spectabile Boreau polyoses grain after 12 mesh sieves.
Above granular preparation is a most preferred embodiment of the present invention, and the preparation method is simple, easy administration.But be not limited to granular preparation, can Chinese medicine composition or its extract of treatment mellitus be processed the preparation that treatment clinically needs, in preparation, add acceptable carrier on the pharmaceutics, the preparation method of preparation is an ordinary method.For example, can the Sedum spectabile Boreau polysaccharide be prepared into paste, or pill processed by the adding tackiness agent or compressing tablet is processed tablet, also can acceptable carrier on Rhizoma Coptidis extract and the pharmaceutics be processed electuary, capsule etc.These extracts close preparation and have identical effective constituent with Chinese medicine composition, and its result of treatment is identical.
Embodiment 7
Below the effect of the scorpion polysaccharide treatment mellitus of the present invention preparation is carried out detailed description.
Choose 100 of clean mouse; Fasting 12 hours, 80 mouse of picked at random, every mouse is pressed 250mg/kg abdominal injection tetraoxypyrimidine solution; Injection is 3 days continuously; Fasting is 12 hours after 3 days, measures mouse blood sugar, confirms as the successful diabetic mice of modeling with the mouse of fasting blood sugar >=11.1mmol/L.30 mouse of picked at random from modeling success mouse; Be divided into 4 groups; Be respectively negative control group, positive controls, Sedum spectabile Boreau polysaccharide low dose group and Sedum spectabile Boreau polysaccharide high dose group; Wherein press every of positive controls every day 200mg/kg and irritate and feed Walaphage, press every of Sedum spectabile Boreau polysaccharide low dose group every day 200mg/kg and irritate and feed the present invention the Sedum spectabile Boreau polysaccharide of preparation, every of Sedum spectabile Boreau polysaccharide high dose group every day press 400mg/kg and irritate the Sedum spectabile Boreau polysaccharide that hello the present invention prepares; Press every of negative control group every day 200mL/kg and irritate and feed water, continuous irrigation was fed 14 days.Remain 20 mouse of not injecting tetraoxypyrimidine, injecting normal saline was injected 3 days continuously, and 10 mouse of picked at random are pressed every of every day 200mL/kg and irritate and feed water as the normal control group after 3 days, and continuous irrigation was fed 14 days.Measure the fasting blood sugar of handling the back mouse, the result is as shown in table 1:
The experiment effect of table 1 Sedum spectabile Boreau polysaccharide treatment mellitus
Can know that from table 1 the mouse blood sugar value of normal control group does not change basically, though the mouse blood sugar value of negative control group has decline, fall is little, blood glucose value about 2.2 mmol/L that on average descend; The positive controls blood glucose value reduces obviously, blood glucose value about 9.0 mmol/L that descend; Sedum spectabile Boreau polysaccharide low dose group and the high dose group blood glucose value of irritate feeding the Sedum spectabile Boreau polysaccharide all have decline, and wherein Sedum spectabile Boreau polysaccharide low dose group range of decrease degree is little, but that Sedum spectabile Boreau polysaccharide high dose group blood glucose value descends is obvious, the blood glucose value 6.1mmol/L that on average descends.The result shows that the Sedum spectabile Boreau polysaccharide of high dosage has hypoglycemic activity, can be used to treat mellitus.
Explanation is at last; Above embodiment is only unrestricted in order to technical scheme of the present invention to be described; Although through invention has been described with reference to the preferred embodiments of the present invention; But those of ordinary skill in the art should be appreciated that and can make various changes to it in form with on the details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (7)
1. the preparation method of Sedum spectabile Boreau polysaccharide is characterized in that, may further comprise the steps:
A. pre-treatment
Sedum spectabile Boreau is pulverized, and medicinal powder adds the refluxing extraction device, and adding volume(tric)fraction is the ethanol of 60-95%, and refluxing extraction is filtered, and abandons filtrating, adds the ethanol that volume(tric)fraction is 60-95% again, and refluxing extraction is filtered, and collects filter residue, promptly gets the pre-treatment Sedum spectabile Boreau;
B. extract
Step a gained pre-treatment Sedum spectabile Boreau is added water, is under the 60-90 ℃ of condition in temperature, extracts, and filters, and collects filtrating, time extracting solution of winning; In the dregs of a decoction, adding water again, is under the 60-90 ℃ of condition in temperature, extracts, and filtrating is collected in filtration, gets extracting solution for the second time, merges filtrating twice, gets crude extract;
C. concentrate
Step b gained crude extract is concentrated, carries out centrifugally then, collect supernatant, liquid concentrator;
D. alcohol precipitation
With adding ethanol to ethanol volume(tric)fraction in the step c gained liquid concentrator is 60-85%, suction filtration, and collecting precipitation will precipitate and use sherwood oil, acetone, absolute ethanol washing successively, and drying gets the Sedum spectabile Boreau Crude polysaccharides;
E. purifying
Steps d gained Sedum spectabile Boreau Crude polysaccharides is water-soluble, add protein liquid removal, thorough mixing leaves standstill; Collect water, in aqueous phase liquid, add gac, constant-temperature shaking, centrifugal; Collect supernatant, in interception is the dialysis tubing of 5000-20000Da, dialyse, lyophilize, the Sedum spectabile Boreau polysaccharide; Said protein liquid removal is chloroform and propyl carbinol mixture, and the volume ratio of chloroform and propyl carbinol is 4:1.
2. according to the preparation method of the said Sedum spectabile Boreau polysaccharide of claim 1, it is characterized in that: said step a is the ethanol of 70-95% for adding the volume(tric)fraction that is equivalent to Sedum spectabile Boreau weight 1-10 times, refluxing extraction; Filter; Abandon filtrating, adding and being equivalent to Sedum spectabile Boreau weight 1-10 volume(tric)fraction doubly is the ethanol of 70-95%, refluxing extraction; Filter, abandon filtrating;
Said step b is equivalent to Sedum spectabile Boreau weight 5-30 water doubly for adding, and is under the 80-90 ℃ of condition in temperature, extracts 1-3 hour, and add again and be equivalent to Sedum spectabile Boreau weight 5-30 water doubly, be under the 60-90 ℃ of condition in temperature, extracted 1-3 hour, filter, collect filtrating;
Said step c is concentrated into 1-3 that liquid concentrator is equivalent to Sedum spectabile Boreau weight doubly; Said centrifugal for being not less than 3000 rev/mins at rotating speed, centrifugally being no less than 10 minutes;
Said steps d is 70-85% for adding absolute ethyl alcohol to ethanol volume(tric)fraction;
Said step e is equivalent to Sedum spectabile Boreau Crude polysaccharides weight 10-40 water doubly for adding, and adds and is equivalent to volume of water 1/6-1/4 protein liquid removal doubly, thorough mixing; In temperature is hold over night under 4 ℃ of conditions, collects water, and adding gac to quality of activated carbon mark is 1%-10%; In temperature is constant-temperature shaking at least 1 hour under 40 ℃ of conditions; Being not less than 3000 rev/mins, centrifugally being no less than 10 minutes at rotating speed, collect supernatant, is dialysis at least 48 hours in the dialysis tubing of 8000-15000Da in interception; Lyophilize gets the Sedum spectabile Boreau polysaccharide.
3. according to the preparation method of the said Sedum spectabile Boreau polysaccharide of claim 1, it is characterized in that:
Said step a is 95% ethanol for adding the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau volumes, refluxing extraction 1-3 hour, filters; Abandon filtrating, add the volume(tric)fraction be equivalent to 5 times of Sedum spectabile Boreau volumes again and be 95% ethanol, refluxing extraction 1-3 hour; Filter, abandon filtrating;
Said step b is under 85 ℃ of conditions for adding the water that is equivalent to 20 times of Sedum spectabile Boreau weight in temperature, extracts 1.5 hours, in the dregs of a decoction, adds the water that is equivalent to 20 times of Sedum spectabile Boreau volumes again, is under 85 ℃ of conditions in temperature, extracts 1.5 hours, filters, and collects filtrating;
Said step c is concentrated into liquid concentrator to be equivalent to 2 times of Sedum spectabile Boreau weight, is 3000 rev/mins, centrifugal 30 minutes at rotating speed;
Said steps d is 80% for adding absolute ethyl alcohol to ethanol final concentration volume(tric)fraction;
Said step e adds the protein liquid removal that is equivalent to 1/5 times of volume of water, thorough mixing again for adding the water that is equivalent to 30 times of Sedum spectabile Boreau Crude polysaccharides weight; In temperature is hold over night under 4 ℃ of conditions, collects water, and adding gac to quality of activated carbon mark is 1.5%; In temperature is constant-temperature shaking 1 hour under 40 ℃ of conditions, is 3000 rev/mins, centrifugal 10 minutes at rotating speed, collects supernatant; In interception is dialysis 48 hours in the dialysis tubing of 8000-15000Da, lyophilize, the Sedum spectabile Boreau polysaccharide.
4. the Sedum spectabile Boreau polysaccharide that utilizes each described preparation method of claim 1-3 to make.
5. the preparation that contains the said Sedum spectabile Boreau polysaccharide of claim 4.
6. according to the said preparation of claim 5, it is characterized in that: said preparation is a granular preparation.
7. said Sedum spectabile Boreau polysaccharide of claim 4 or the said preparation of claim 5 application in preparation treatment diabetes medicament.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110458709 CN102408493B (en) | 2011-12-31 | 2011-12-31 | Sedum spectabile polysaccharide preparation method, and product and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110458709 CN102408493B (en) | 2011-12-31 | 2011-12-31 | Sedum spectabile polysaccharide preparation method, and product and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102408493A true CN102408493A (en) | 2012-04-11 |
| CN102408493B CN102408493B (en) | 2013-01-02 |
Family
ID=45910875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110458709 Expired - Fee Related CN102408493B (en) | 2011-12-31 | 2011-12-31 | Sedum spectabile polysaccharide preparation method, and product and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102408493B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103768111A (en) * | 2014-02-26 | 2014-05-07 | 李嘉浩 | Chinese patent medicine for treating poisonous bee stings and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3099992B1 (en) * | 2019-08-21 | 2022-03-25 | Laboratoires De Biologie Vegetale Yves Rocher | COSMETIC USE OF AN EXTRACT OF SEDUM SPECTABILE AS A SLIMMING AGENT, AND COMPOSITIONS CONTAINING SUCH AN EXTRACT |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642186A (en) * | 2009-07-31 | 2010-02-10 | 广州市希普生物饲料有限公司 | Preparation method for feed without antibiotics |
-
2011
- 2011-12-31 CN CN 201110458709 patent/CN102408493B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101642186A (en) * | 2009-07-31 | 2010-02-10 | 广州市希普生物饲料有限公司 | Preparation method for feed without antibiotics |
Non-Patent Citations (1)
| Title |
|---|
| 陈长勋等: "蝎子草根提取液药理作用探索", 《中药材》, vol. 16, no. 11, 27 November 1993 (1993-11-27), pages 28 - 31 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103768111A (en) * | 2014-02-26 | 2014-05-07 | 李嘉浩 | Chinese patent medicine for treating poisonous bee stings and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102408493B (en) | 2013-01-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107158063B (en) | Enteric-coated pellet prepared from honeysuckle effective part composition and preparation method thereof | |
| CN103494875B (en) | Application of cyclocarya paliurus extract in preparation of medicament for preventing and treating leukemia | |
| CN102030834B (en) | Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide | |
| CN101396384B (en) | Asiatic centella extract and preparation methode thereof | |
| CN112870236B (en) | Flavone effective part of abelmoschus manihot and preparation method and application thereof | |
| CN102836188A (en) | Folium microcotis total flavone extract and preparation method and application thereof | |
| CN101040950B (en) | Method of preparing compound Houttuynia cordata dropping pills | |
| CN105362311A (en) | Method for extracting and separating volatile oil, flavone and polysaccharide of abelmoschus manihot flowers | |
| CN101497637B (en) | Method for extracting high-purity scutellarin from breviscpini | |
| CN103505492B (en) | A kind of method using ultrafiltration to prepare high anti-oxidation value Radix Rhodiolae extract | |
| CN100446780C (en) | Method for preparing injection preparation of freeze drying powder of Xuesaitong | |
| CN102408493B (en) | Sedum spectabile polysaccharide preparation method, and product and application thereof | |
| CN1654060A (en) | 'Kidney-Benefiting' glucose injection and its preparing process | |
| CN111803531A (en) | Preparation method and application of dandelion leaf water-soluble crude extract | |
| CN1171603C (en) | Granule for clearing away heat and dispelling watness-evil | |
| CN101637550B (en) | Chinese fanpalm seed polysaccharide extract and preparation method and purpose thereof | |
| CN102477105A (en) | Method for combined preparation of polysaccharide and flavone by using pumpkin stems and leaves as raw material | |
| CN101972284B (en) | Preparation method of pine needle fat soluble extract and application thereof in preparing drug for treating gastric ulcer | |
| CN1072018C (en) | Traditional Chinese medicine compound medicine for giving-up drug-taking and its compounding method | |
| CN101721449B (en) | Method for preparing lindley eupatorium herb general flavone by using attapulgite adsorption method | |
| CN101564418B (en) | Pharmaceutical composition for treating oral ulcer | |
| CN1644203A (en) | Oral preparation of red common stone crop polysaccharide and its making method | |
| CN101108197B (en) | Total polyoses from inula flower and use in regulating or treating diabetes and complication and immunoregulation | |
| CN1456337A (en) | Balsam pear products containing multiple hypoglycemic active composition and preparation thereof | |
| CN1943647A (en) | The method for preparing triterpenic acid extract from the loquat leaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130102 Termination date: 20151231 |
|
| EXPY | Termination of patent right or utility model |