CN102391946A - Plunger sleeve and automatic cell processor therewith - Google Patents
Plunger sleeve and automatic cell processor therewith Download PDFInfo
- Publication number
- CN102391946A CN102391946A CN2011103565700A CN201110356570A CN102391946A CN 102391946 A CN102391946 A CN 102391946A CN 2011103565700 A CN2011103565700 A CN 2011103565700A CN 201110356570 A CN201110356570 A CN 201110356570A CN 102391946 A CN102391946 A CN 102391946A
- Authority
- CN
- China
- Prior art keywords
- plunger bushing
- film
- base
- communicated
- plunger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention provides a plunger sleeve. The plunger sleeve is characterized in that: the plunger sleeve is arranged on a pedestal; the bottom of the plunger sleeve is provided with a first plunger sleeve channel and a second plunger sleeve channel; the pedestal is provided with a first pedestal channel and a second pedestal channel; the first plunger sleeve channel is communicated with the first pedestal channel to form a channel A; the second plunger sleeve channel is communicated with the second pedestal channel to form a channel B; the joint of the second plunger sleeve channel and the second pedestal channel is provided with a microporous filtration film; the channel A has a pore size which allows cells which cannot flow through the microporous filtration film to flow through the channel A; an underfilm support is arranged between a part under the microporous filtration film and the pedestal; and the underfilm support is provided with at least one liquid through holes communicated with the second pedestal channel. The invention also provides an automatic cell processor with the plunger sleeve. The automatic cell processor allows access requirements of various processing liquids to be satisfied, the plunger sleeve to be conveniently removed from the automatic processor and arranged on the automatic processor, and the automatic processing to be easily realized.
Description
Technical field
The present invention relates to cell and clean and the cell dyeing technical field, specifically is a kind of plunger bushing and the cell robotization treatment unit that comprises this plunger bushing.
Background technology
At present, known cell cleaning and cell dyeing generally adopt centrifugation or filtering under pressure mode.Yet; Inventor's process in realizing process of the present invention is discovered: centrifugation is for the cell that can not effectively sink to the bottom; Be prone to when removing the supernatant waste liquid cell is removed, cause cell loss during washed cell, more difficult when separating the cell mixing of different volumes size.The filtering under pressure mode is blocking material with the film, uses the syringe equipressure to make solution pass syringe needle filter film, will need small-molecule substance percolations such as isolated antibody or other staining agent to cross film; (organoid is the microstructure that has certain 26S Proteasome Structure and Function in the tenuigenin, and the organoid in the cell mainly contains: plastosome-endoplasmic reticulum-centrosome-chloroplast(id), golgi body-rrna etc. for film entrapped cell or organoid; They have formed the substruction of cell; Make cell can work normally-turn round), realize cell washing, owing to will add and discharge various liquid repeatedly in the operating process; Process is complicated, is difficult to realize automated operation.
Summary of the invention
The objective of the invention is to overcome the deficiency that existing cell cleans and cell dyeing is technological; A kind of plunger bushing is provided and comprises the cell robotization treatment unit of this plunger bushing; It is employed in and has on the millipore filtration that can allow can not be from the method for the passage of the cell percolation of percolation on the millipore filtration; Cooperate correspondent control circuits and structure, realize robotization.
A kind of plunger bushing; Be used to be installed in base; The bottom of said plunger bushing is provided with the first plunger bushing passage and the second plunger bushing passage; Said base offers first base passages and second base passages, and wherein said first plunger bushing passage and said first base passages are communicated with formation passage A, and said second plunger bushing passage and said second base passages are communicated with the formation channel B; The junction of said second plunger bushing passage and said second base passages is provided with millipore filtration; The aperture of said passage A is set to allow and can not below the millipore filtration 8 and between the base film lower bracket be set from the cell percolation of percolation on the said millipore filtration, offers the liquid through-hole that at least one is communicated with said second base passages on the film lower bracket.
A kind of cell robotization treatment unit; It is characterized in that: comprise plunger bushing, plunger, base; Said plunger is located in the plunger bushing; Said plunger bushing is installed on the base, and the bottom of said plunger bushing is provided with the first plunger bushing passage and the second plunger bushing passage, and said base offers first base passages and second base passages; Wherein said first plunger bushing passage and said first base passages are communicated with formation passage A; Said second plunger bushing passage and said second base passages are communicated with the formation channel B, and the junction of said second plunger bushing passage and said second base passages is provided with millipore filtration, and the aperture of said passage A is set to allow can not be from the cell percolation of percolation on the said millipore filtration; The film lower bracket is being set below the millipore filtration and between the base, is offering the liquid through-hole that at least one is communicated with said second base passages on the film lower bracket.
Plunger bushing provided by the invention and the aperture of said passage A that comprises the cell robotization treatment unit of this plunger bushing are set to allow can not be from the cell percolation of percolation on the said millipore filtration; Can realize injecting treatment solution or pending enchylema from the millipore filtration top; Said second plunger bushing passage and said second base passages are communicated with the channel B that forms can realize that injecting treatment solution from the millipore filtration below (can make cell leave millipore filtration and resuspension; The action function that stirring is arranged), can satisfy the entering requirement of various treatment solutions like this; In addition, plunger bushing provided by the invention can take off and install from the robotization treatment unit easily, and it can be independent of outside the said robotization treatment unit; Under the mating reaction of time variable control and respective execution mechanisms, can add automatically or the discharge corresponding liquid easily by the requirement of cell cleaning and cell dyeing flow process; Its low cost of manufacture also can prevent sample contamination, realizes the robotization processing easily; Use flexibly, applied range adapts to multiple use.
Description of drawings
Fig. 1 is the stereographic map that first embodiment of the invention comprises the plunger bushing of plunger;
Fig. 2 is the stereographic map that the present invention's first embodiment plunger bushing can be seen the bottom;
Fig. 3 is the vertical view of the present invention's first embodiment plunger bushing;
Fig. 4 is the stereographic map of the present invention's first another direction of embodiment plunger bushing;
Fig. 5 is the upward view of the present invention's first embodiment plunger bushing;
Fig. 6 A is the stereographic map of the present invention's first embodiment plunger bushing;
Fig. 6 B is the stereographic map that the present invention's first embodiment is provided with the plunger bushing of another kind of film lower bracket;
Fig. 7 is the structural representation of the millipore filtration installed in the present invention's first embodiment plunger bushing;
Fig. 8 is the sectional arrangement drawing of the present invention's second embodiment plunger bushing;
Fig. 9 is the part sectioned view of the present invention's second embodiment plunger bushing;
Figure 10 is that the device of second embodiment of the invention is formed synoptic diagram;
Figure 11 is that the device of third embodiment of the invention is formed synoptic diagram;
Figure 12 is that the system of one of them embodiment of the present invention constitutes synoptic diagram;
Figure 13 is the cell automated process flow synoptic diagram of Figure 10.
Among the figure: the 1-plunger bushing; The 2-plunger bushing is communicated with platform; 3-plunger bushing bottom outlet; 4-is communicated with the platform through hole; 4A-is communicated with platform and flexibly connects pad; 5-film outer gasket; The 6-film is interior along gasket; 7-film ditch; The 8-millipore filtration; 9,9A-film lower bracket; The 20-plunger; The 21-impeller; The 22-gasket; The 30-base; Cavity under communicating aperture, the 34-film under communicating aperture, 33-second film on communicating aperture, the 32-film under 31-first film; Cavity is communicated with platform under the 34A-film; Cavity communicating aperture under the 34B-film; The 35-gasket; The 36-whorl of base; 36A-plunger bushing screw thread; The 40-soft magnetic bodies; The 50-electro-magnet; The 51-electromagnetic core; The 52-magnet coil; 60-elevating lever lower lock block; The 61-elevating lever; 62-elevating lever motor; Liquid adding vessel on the 70a-film; Liquid adding vessel on the 70b-film; Liquid adding vessel on the 70c-film; Liquid adding vessel under the 71a-film; Liquid adding vessel under the 71b-film; Liquid adding vessel under the 71c-film; The 72-pneumatic supply; The 73-waste fluid container; 80a, 80b, 80c-SV; 81a, 81b, 81c-SV; The 82-SV; 83,84-liquor pump; 85-pressure detection unit; 90,91,92,93,94,95,96,97-SV; 100,101,102,103,104-pipeline; The 901-liquid through-hole, the 105-front shroud.
Embodiment
To combine the accompanying drawing among the present invention below, the technical scheme among the present invention carried out clear-intactly description.
Please refer to Fig. 1, plunger 20 is installed in the first embodiment of the invention plunger bushing 1, the tail end of plunger 20 is provided with impeller 21.Please in the lump with reference to figure 2-5, said plunger bushing 1 is a cast device, and its top is opening end, and the bottom capsule is provided with to the plunger bushing that extends below and is communicated with platform 2, offers in the plunger bushing connection platform 2 to be communicated with platform through hole 4; Also be provided with plunger bushing bottom outlet 3 on the said plunger bushing 1 bottom capsule; Concrete; Can be with reference to figure 3; Be interval with many groups on the said plunger bushing 1 bottom capsule and be the fan-shaped plunger bushing bottom outlet 3 that shape is arranged of gradually opening, wherein said connection platform through hole 4 is as the first plunger bushing passage, and plunger bushing bottom outlet 3 is as the second plunger bushing passage.
Please refer to Fig. 6 A; The below of millipore filtration 8 is equipped with film lower bracket 9, and film lower bracket 9 can be connected with the plunger bushing bottom through the method (or additive method) of welding, also can be through the installation that is threaded; Through the then conveniently replaced different millipore filtration of being connected and installed of engage thread; Offering at least one liquid through-hole on the film lower bracket 9, is the film lower bracket 9 of thread connecting mode shown in Fig. 6 A, offers a plurality of liquid through-holes on it; Its with said plunger bushing 1 bottom capsule on the plunger bushing bottom outlet 3 offered corresponding, the bottom that said plunger bushing is communicated with platform 2 is provided with and is communicated with platform and flexibly connects pad 4A.
Plunger bushing is communicated with the geometric centre that platform 2 is arranged on plunger bushing among Fig. 6 A; Can easily plunger bushing be connected with base through spiral way like this; Certainly, also can plunger bushing be connected (shown in Fig. 6 B) with base 30, at this moment through snap fit; Plunger bushing is communicated with the geometric centre that platform 2 can be arranged on plunger bushing; This moment, film lower bracket 9A was connected with the plunger bushing bottom through the method for welding, on film lower bracket 9A, was provided with cavity connection platform 34A under the film, offered cavity communicating aperture 34B under the film among the cavity connection platform 34A under the said film.Cavity communicating aperture 34B is used under the film of said base 30 cavity 34 and is communicated with that liquid adding vessel is connected under back and the film under the said film, realizes injection treatment solution under the film.The solution plunger bushing is communicated with to have in the platform 2 and is communicated with platform through hole 4 under the film, can plunger bushing be communicated with platform 2 by the design of GB syringe size, on the markets such as convenient and syringe needle easily the standard equipment of purchase be connected.This moment, it was that (millipore filtration is when wetted for a multi-functional syringe; Because the surface tension effects of liquid, applied pressure less than the condition that overcomes surface tension effects under, the millipore filtration barrier gas is passed through; Be equivalent to closed state), can be used for drawing or discharging sample solution.
Please refer to Fig. 8 and Fig. 9, in another embodiment, the plunger bushing 1 that inside is equipped with plunger 20 is fixed on the base 30, and the head of plunger 20 is equiped with gasket 22, can further guarantee the resistance to air loss between plunger 20 and plunger bushing 1 inner side-wall.As shown in Figure 9, said plunger bushing 1 bottom capsule is provided with to the plunger bushing that extends below and is communicated with platform 2, offers in the plunger bushing connection platform 2 to be communicated with platform through hole 4, and said connection platform through hole 4 is as the first plunger bushing passage; Said plunger bushing 1 bottom capsule is provided with plunger bushing bottom outlet 3, and said plunger bushing bottom outlet 3 is as the second plunger bushing passage.
Millipore filtration roughly has four kinds of structural patterns because manufacture is different, promptly sieves formula structure, network structure, intermediate structure, anisotropic structure, wherein sieves the formula structure and catches particle through face, and fenestra is prone to stop up, and back washing is easy; Network structure is trapped particle in mesh, and filtration velocity is fast, and total filtration yield is big, the back washing difficulty; The film toughness of intermediate structure is big, and other characteristics are between sieve formula structure and network structure; Total filtration yield of anisotropic structure is big, and the table aperture is big, and the aperture, lining is little.Concern that introduction is following between the purposes of millipore filtration and the aperture of filter membrane and the purposes:
The purposes of millipore filtration: microbioassay in the liquid, topsoil inspection, sterile test; Microorganism checking in the air, microscopic examination, particulate analysis; Gravimetric analysis, colorimetric analysis, fluid contamination detects; The chemically reactive reaction kit is analyzed, and dissolves to be situated between and non-molten Jie's material contrast, decontamination chamber and working suit pollution detection thereof; The filtration of degerming, blood filtration, plasma separation, serum, tissue culture medium and other biochemical preparations and degerming.
Relation between aperture of millipore filtration (um) and the purposes:
3.0～8.0: solvent, reagent, lubricating oil, photo-resist clarification filtration.Remove the pre-filtering of 1.0 following particles in the fluid; Cytologic diagnosis; Particulate detects in the liquid; Remove chlorella and Lan Se algae in the fresh water;
0.8～1.0: quantitatively the detecting of yeast, mould; Remove yeast, mould in beer, wine, liquid glucose, the soda pop; General clarification filtration removes and degerms (referring to anthrax-bacilus, staphylococcus).Purifying air;
0.4～0.6: general clarification filtration, remove degerm (intestinal bacteria, cholera, typhoid fever, typhus fever, green pus bacterium, tetanolysin); The capture fibrous magnesium silicate filters, particulate macroanalysis, bacteria total amount analysis;
0.2: bacterium all captures filtration (natural viral, vaccinia virus); Bacteria total amount is analyzed; Plasma separation;
0.08～0.10: virus filtration (influenza, rabies virus etc.); Ultrapure water finally filters, artificial lung, plasma purification;
0.03～0.05: bacteriophage is filtered (poliomyelitis virus, influenza virus); High molecular weight protein filters; Sterilized water is made.
Communicating aperture 32 is as first base passages on the said film, under the said film under cavity 34 and the film that is communicated with it communicating aperture (31,33) as second base passages.After plunger bushing 1 is installed on the base 30; The part-structure of plunger bushing 1 bottom (plunger bushing 1 bottom and plunger bushing are communicated with platform 2) inserts the cavity that base 30 is offered; Communicating aperture 32 (first base passages) is communicated with such first plunger bushing passage and first base passages connection formation passage A on the connection platform through hole 4 (the first plunger bushing passage) that plunger bushing is communicated with platform 2 and the film.Successively millipore filtration 8, film lower bracket 9 are packed into; Said plunger bushing bottom outlet 3 (the second plunger bushing passage) is through communicating aperture (second base passages) connection under cavity 34 under the film on millipore filtration 8, film lower bracket 9 and the said base 30 and the film that is communicated with it; Wherein the second plunger bushing passage and second base passages form channel B, and millipore filtration 8 is arranged on the junction of the second plunger bushing passage and second base passages.The aperture of said passage A is set to allow can not be from the cell percolation of percolation on the said millipore filtration 8.The bottom of said plunger bushing 1 is interval with the film ditch 7 of groove-like in the form of a ring, and being convenient to attach aftertreatment fluid with millipore filtration 8 can fully contact with millipore filtration 8, helps treatment solution and overflows from millipore filtration 8.
Please refer to Figure 10; Communicating aperture 32 is connected through liquid adding vessel (70a, 70b, 70c) on transfer lime and the film on the film of offering on the said base 30; Enchylema (for example scavenging solution, antibody, damping fluid or staining fluid etc.) and the pending enchylema that is used to handle cell like this can be from liquid adding vessel on the film (70a, 70b, 70c) from said passage A (be on the film communicating aperture 32 and be communicated with platform through hole 4 and be communicated with the passage that forms) gets into the enclosed space between millipore filtration 8 and the plunger 20, realizes the injection enchylema (for example scavenging solution, antibody, damping fluid or staining fluid etc.) from millipore filtration 8 tops.
With communicating aperture under one of them film that cavity 34 under the said film is communicated with; Promptly communicating aperture 33 is connected through liquid adding vessel (71a, 71b, 71c) under transfer lime and the film under second film; The enchylema (for example scavenging solution, antibody, damping fluid or staining fluid etc.) that is used to handle cell like this can be from liquid adding vessel under the film successively through behind cavity 34, the film lower bracket 9 under communicating aperture 33, the film under second film; Pass millipore filtration 8 back and get into enclosed space between millipore filtrations 8 and the plunger 20 (wherein under second film under communicating aperture 33, the film cavity 34 is communicated with the formation channel B with plunger bushing bottom outlet 3) through plunger bushing bottom outlet 3, realization is injection enchylema (for example scavenging solution, antibody, damping fluid or staining fluid etc.) from millipore filtration 8 belows.With communicating aperture under the another one film that cavity 34 under the said film is communicated with; I.e. communicating aperture 31 under first film; It is connected with waste fluid container 73 through transfer lime, after the treatment solution pair cell is finished dealing with, after treatment solution can pass millipore filtration 8 from the enclosed space between millipore filtration 8 and the plunger 20; Deliver to waste fluid container 73 through 34 backs of cavity under film lower bracket 9, the film through crossing transfer lime successively, realize the collection of waste liquid.
Passage A and channel B all can have liquid-flow under the effect of pressure; Pressure source can be the motion of inserting the plunger 20 of plunger bushing 1; It also can be the outside liquid pump (like rampump, peristaltic pump, toothed gear pump etc.) that is communicated with plunger bushing 1; Shown in Figure 10 be with film on liquor pump 83 and 84 on liquid adding vessel (71a, 71b, 71c) is communicated with under liquid adding vessel (70a, 70b, 70c) and the film the transfer lime; Pressure source can also be moving up and down and both actings in conjunction of outside liquid pump (83,84) of plunger 20, pressure source also can be with film on liquid adding vessel is communicated with under liquid adding vessel and the film pneumatic supply 72, for example nitrogen pot.
Shown in figure 10; Liquid adding vessel (71a, 71b, 71c) can be realized pressurization through pneumatic supply 72 under liquid adding vessel on the film (70a, 70b, 70c) and the film, with treatment solution pressurization injection channel A and the channel B in the liquid adding vessel (71a, 71b, 71c) under liquid adding vessel on the film (70a, 70b, 70c) and the film.The transfer lime of liquid adding vessel on the film (70a, 70b, 70c) is provided with SV (80a, 80b, 80c), and the transfer lime of liquid adding vessel under the film (71a, 71b, 71c) is provided with SV (81a, 81b, 81c).Also be provided with SV 82 on the conveying transfer lime that communicating aperture 31 is connected under waste fluid container 73 and first film.
When with pneumatic supply 72 (for example nitrogen pot) during as pressure source; Liquor pump (83,84) can; Purpose is can the liquid in the liquid adding vessel under liquid adding vessel on the film and the film be pressed into or carry out the cleaning of pipeline on demand; Be scavenging solution in the 71a for example, when pipe blow-through, can open SV 81a and SV 82; The wash liquid stream enclosed space that cavity 34, film lower bracket 9, millipore filtration 8, plunger bushing bottom outlet 3 get into millipore filtration 8 and plunger 20 bottoms under communicating aperture 33, film under transfer lime, second film carries out cell and cleans; Waste liquid after cleaning is under the effect of plunger 20, and the communicating aperture 31 last waste fluid containers 73 that arrive are realized the collection of waste liquid under space 34, first film under millipore filtration 8, film lower bracket 9, the film.The air pressure that pneumatic supply 72 is exported can monitored and control to pneumatic supply 72 and pressure detection unit 85 in real time.
The liquid volume that treatment solution is injected in general control is to be determined by the relative position of plunger 20 in plunger bushing 1.The motion of plunger 20 can be assisted through external force, and shown in figure 10, elevating lever motor 62 is divided into elevating lever 61, and elevating lever 61 is connected with elevating lever lower lock block 60.When adding treatment solution, plunger 20 is upwards pushed away under pressure, at this moment; Elevating lever motor 62 drives elevating lever 61; It is up to drive elevating lever lower lock block 60, on elevating lever lower lock block 60, can be provided with device for measuring force (for example semiconductor pressure sensor), and plunger upwards effect can be detected by this device for measuring force; The position of elevating lever lower lock block 60 cooperates pressure detection; Can indicate the capacity of the treatment solution in the plunger bushing 1, use pressurized gas (or common liq pump) to get final product like this, needn't use and accurately to control the treatment solution capacity, the expensive liquor pump that gets in the plunger bushing 1 as pressure source.
When aux. pressure being provided in need up process at plunger 20; Can increase electro-magnet 50 60 times at the elevating lever lower lock block; Magnet coil 52 is wrapped on the electromagnetic core 51 in the electro-magnet 50, and soft magnetic bodies 40 (like iron etc.) on the card of the upper surface position on the plunger 20 impeller 21 of plunger 20 tail ends (promptly) simultaneously is when magnet coil 52 is switched on; Electro-magnet 50 can produce suction soft magnetic bodies 40 is tightly held, and aux. pressure is provided; During outage then electro-magnet 50 separate with soft magnetic bodies 40.The effect of electro-magnet 50 is to hold the soft magnetic bodies that is installed on the plunger 20 can assist the stay column plug, and is easier when making treatment solution get into plunger bushing 1.
Please combine with reference to Figure 11; Plunger bushing 1 of the present invention is installed to is performed such when carrying out the cell clean in the robotization treatment unit: behind the power supply that starts the machine; Open machine front shroud 105, elevating lever 61 rises to the extreme higher position with elevating lever lower lock block 60, and can be installed in plunger bushing 1 on the base 30 easily and (earlier plunger 20 inserted plunger bushing 1 generally speaking this moment; Draw cell or biological organization sample, install again behind the excluding air).After installing, shut the front shroud 105 of machine, configure treatment scheme, start the sample process flow process, machine can be handled by setting process automatically, sends prompting after finishing dealing with.
Please refer to Figure 13, the introduction of cell cleaning operation process is following:
Step 1: cell sample introduction; Concrete, cell to be cleaned is injected the enclosed space (film injects) between millipore filtration 8 and the plunger 20 from passage A;
Step 2: add scavenging solution; Concrete, scavenging solution is injected from channel B, inject scavenging solution from millipore filtration 8 belows, make cell leave millipore filtration 8 and resuspension.
Step 3: discharge scavenging solution, effectively cell is separated with scavenging solution rapidly;
After step 3 is accomplished, return performing step 2, promptly after each discharge opeing, add scavenging solution again, make enchylema dilute (repeatedly capable of circulation, the round-robin number of times is set according to actual needs, and last circulation does not add scavenging solution) again.
Please combine with reference to Figure 12, the robotization treatment unit shown in it is used for isolating the small volume cell from pending enchylema, for example from blood, separates thrombocyte, white corpuscle, red corpuscle.By plunger 20 in plunger bushing 1 upwards motion directly manually suck biological specimen (this moment, plunger bushing 1 was not installed on the machine as yet); The biological specimen that perhaps is used to separate synthetic operation is by in pipeline 101 first plunger bushing of entering 1a, and all SVs are in closing condition, carry out following steps then:
(1) opens SV 91 uplinks; Plunger in first plunger bushing 1a is up, draws sample, and then adds cleaning buffer solution by pipeline 101, SV 91 uplinks; Biological specimen is got in the plunger bushing 1a (comprising the biological specimen in the pipeline space) fully, close all SVs; If the millipore filtration of plunger bushing 1a bottom has been blocked by cell filtering the time, can inject cleaning buffer solution from pipeline 100 and enter, make the cell suspension on the millipore filtration.
(2) open SV 92 downlinks and SV 93 uplinks; Plunger in the 1a is descending, and the plunger in the 1b is simultaneously up, on SV 92 downlinks, pipeline 102 and SV 93, is advanced into the cell of small volume in second plunger bushing 1b; Close all SVs; Open SV 91 downlinks and SV 93 uplinks again, add cleaning buffer solution, close all SVs through pipeline 101; What stay in the 1a plunger bushing is big volume cell;
(3) open SV 94 downlinks and SV 95 uplinks; Plunger in the descending 1c of plunger in the 1b is simultaneously up; With the cell of inferior small volume in SV 94 downlinks, pipeline 103, SV 95 uplinks get into the 3rd plunger bushing 1c; Close all SVs, open SV 91 downlinks, 95 uplinks, close all SVs through pipeline 101 adding cleaning buffer solutions; What stay in the 1b plunger bushing is the sample or the organoid of small volume cell or small volume;
(4) open SV 97, the plunger in the 1c is descending, and the cell of small volume is more got in the cell returnable (for example waste fluid container 73) through pipeline 104; What stay in the 1c plunger bushing is time small volume cell or organoid.
Plunger bushing provided by the invention and comprise the cell robotization treatment unit of this plunger bushing owing on plunger bushing, offer the first plunger bushing passage and the second plunger bushing passage; Corresponding with it simultaneously offers first base passages and second base passages at base; The junction of said second plunger bushing passage and said second base passages is provided with millipore filtration; Wherein the first plunger bushing passage and said first base passages are communicated with formation passage A; The aperture of said passage A is set to allow can not be from the cell percolation of percolation on the said millipore filtration; Can realize injecting treatment solution or pending enchylema from the millipore filtration top, said second plunger bushing passage and said second base passages are communicated with the channel B that forms and can realize injecting treatment solution from the millipore filtration below, can satisfy the entering requirement of various treatment solutions like this; The cell place that for example has needs pending cell to be in abundant suspended state; And existing plunger bushing can only inject treatment solution (injecting on the film) by its opening end, and treatment solution can not make cell start from abundant suspended state after getting into plunger bushing like this, can not carry out effective lock out operation by pair cell.
In addition, plunger bushing provided by the invention can take off and install from the robotization treatment unit easily, and it can be independent of outside the said robotization treatment unit; Under the mating reaction of time variable control and respective execution mechanisms, can add automatically or the discharge corresponding liquid easily by the requirement of cell cleaning and cell dyeing flow process; Its low cost of manufacture; Also can prevent sample contamination, realize robotization processing (cell cleans and cell dyeing) easily, use flexibly; Applied range adapts to multiple use.
Plunger bushing provided by the invention and the cell robotization treatment unit that comprises this plunger bushing can be used for the separation of the cell of different volumes size, also can be used for the filtration and the detection of Chinese medicine impurity of Chinese medicine injection aspect; Also can be used for the air sampling aspect.A plurality of said apparatus are assembled on the machine, handle when can realize a plurality of sample, the preposition supporting washing device that it also can be used as stream type cell analyzer is applied to stream type cell analyzer or combination.
The above; Be merely embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technician who belongs to the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.
Claims (10)
1. plunger bushing; It is characterized in that: be used to be installed in base; The bottom of said plunger bushing is provided with the first plunger bushing passage and the second plunger bushing passage; Said base offers first base passages and second base passages, and wherein said first plunger bushing passage and said first base passages are communicated with formation passage A, and said second plunger bushing passage and said second base passages are communicated with the formation channel B; The junction of said second plunger bushing passage and said second base passages is provided with millipore filtration (8); The aperture of said passage A is set to allow to go up from said millipore filtration (8) the cell percolation of percolation, between the below of millipore filtration (8) and base, film lower bracket (9) is set, and offers the liquid through-hole (901) that at least one is communicated with said second base passages on the film lower bracket (9).
2. plunger bushing as claimed in claim 1; It is characterized in that: said plunger bushing is a cast device, and its top is opening end, and the bottom capsule is provided with to the plunger bushing that extends below and is communicated with platform (2); Offer in the plunger bushing connection platform (2) and be communicated with platform through hole (4); Said connection platform through hole (4) is as the said first plunger bushing passage, and said plunger bushing (1) bottom capsule is provided with plunger bushing bottom outlet (3), and said plunger bushing bottom outlet (3) is as the said second plunger bushing passage; The connection platform through hole (4) that the corresponding plunger bushing of said base (30) is communicated with platform (2) is provided with communicating aperture on the film (32); Communicating aperture on the said film (32) is as said first base passages; Said base (30) offers cavity under the film (34) in the below of millipore filtration (8); Communicating aperture is communicated with under cavity under the film (34) and at least one film; Communicating aperture is as said second base passages under cavity under the said film (34) and the film that is communicated with it, and cavity (34) is communicated with under the liquid through-hole (901) of film lower bracket (9) and the said film.
3. plunger bushing as claimed in claim 1 is characterized in that: said plunger bushing and base (30) are for being threaded, and said plunger bushing is communicated with the geometric centre that platform (2) is arranged on plunger bushing.
4. plunger bushing as claimed in claim 1 is characterized in that: said plunger bushing and base (30) fixedly mount through the mode of clamping.
5. cell robotization treatment unit; It is characterized in that: comprise plunger bushing (1), plunger (20), base (30); Said plunger (20) is located in the plunger bushing; Said plunger bushing is installed on the base (30), and the bottom of said plunger bushing is provided with the first plunger bushing passage and the second plunger bushing passage, and said base (30) offers first base passages and second base passages; Wherein said first plunger bushing passage and said first base passages are communicated with formation passage A; Said second plunger bushing passage and said second base passages are communicated with the formation channel B, and the junction of said second plunger bushing passage and said second base passages is provided with millipore filtration (8), and the aperture of said passage A is set to allow to go up from said millipore filtration (8) the cell percolation of percolation; Film lower bracket (9) is being set below the millipore filtration 8 and between the base, is offering the liquid through-hole (901) that at least one is communicated with said second base passages on the film lower bracket (9).
6. cell robotization treatment unit as claimed in claim 5; It is characterized in that: also comprise liquid adding vessel and waste fluid container (73) under liquid adding vessel on the film, the film; Liquid adding vessel and said waste fluid container (73) are connected on said first base passages and the said film; Liquid adding vessel is connected under said second base passages and the said film, on the said film under liquid adding vessel and the said film liquid adding vessel be connected with pressure source.
7. cell robotization treatment unit as claimed in claim 6 is characterized in that: said pressure source is pneumatic supply or liquor pump.
8. like claim 6 or 7 described cell robotization treatment unit; It is characterized in that: said plunger bushing is a cast device, and its top is opening end, and the bottom capsule is provided with to the plunger bushing that extends below and is communicated with platform (2); Offer in the plunger bushing connection platform (2) and be communicated with platform through hole (4); Said connection platform through hole (4) is as the said first plunger bushing passage, and said plunger bushing (1) bottom capsule is provided with plunger bushing bottom outlet (3), and said plunger bushing bottom outlet (3) is as the said second plunger bushing passage; The connection platform through hole (4) that the corresponding plunger bushing of said base (30) is communicated with platform (2) is provided with communicating aperture on the film (32); Communicating aperture on the said film (32) is as said first base passages; Said base (30) offers cavity under the film (34) in the below of millipore filtration (8); Communicating aperture is communicated with under cavity under the film (34) and at least one film; Communicating aperture is as said second base passages under cavity under the said film (34) and the film that is communicated with it, and cavity (34) is communicated with under the liquid through-hole (901) of film lower bracket (9) and the said film.
9. cell robotization treatment unit as claimed in claim 8; It is characterized in that: also comprise the elevating lever lower lock block (60) that is arranged on plunger (20) top; Elevating lever lower lock block (60) is provided with device for measuring force, and elevating lever lower lock block (60) is connected with elevating lever motor (62) through elevating lever (61).
10. cell robotization treatment unit as claimed in claim 9 is characterized in that: electro-magnet (50), soft magnetic bodies (40) on impeller (21) card of plunger (20) tail end are installed below the elevating lever lower lock block (60).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110356570 CN102391946B (en) | 2011-11-11 | 2011-11-11 | Plunger sleeve and automatic cell processor therewith |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110356570 CN102391946B (en) | 2011-11-11 | 2011-11-11 | Plunger sleeve and automatic cell processor therewith |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102391946A true CN102391946A (en) | 2012-03-28 |
| CN102391946B CN102391946B (en) | 2013-01-23 |
Family
ID=45859331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110356570 Expired - Fee Related CN102391946B (en) | 2011-11-11 | 2011-11-11 | Plunger sleeve and automatic cell processor therewith |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102391946B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104931711A (en) * | 2015-06-25 | 2015-09-23 | 马鞍山海普微奇生物科技有限公司 | Method and device for promoting combination of biological reagents and biomolecules |
| CN108277152A (en) * | 2018-04-04 | 2018-07-13 | 徐俊 | The sterile liquid changing device of cell recovery and carry out the method that cell recovery changes liquid using the device |
| CN109877152A (en) * | 2019-03-21 | 2019-06-14 | 贵州民族大学 | A kind of prosthetic device and restorative procedure for heavy metal ion in artificial swamp |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2585236Y (en) * | 2002-08-29 | 2003-11-05 | 江西特康科技有限公司 | Plunger-type syringe sealing structure of haemocytes analyzer |
| CN1833167A (en) * | 2003-05-30 | 2006-09-13 | 国家航天科技研究所“埃斯特万·特拉达斯” | Method and apparatus for the detection of substances or analytes from the analysis of one or more samples |
| CN101506643A (en) * | 2006-07-13 | 2009-08-12 | 海马生物科学公司 | Cell analysis apparatus and method |
| CN201368820Y (en) * | 2009-06-03 | 2009-12-23 | 武汉宏强医疗器械有限公司 | Injector for cell examination |
| CN101797549A (en) * | 2009-02-11 | 2010-08-11 | 苏舍米克斯帕克有限公司 | Cartridge piston with exhaust apparatus |
| CN201600378U (en) * | 2009-12-31 | 2010-10-06 | 南京劳拉电子有限公司 | Multichannel injector |
| CN102220224A (en) * | 2011-05-19 | 2011-10-19 | 汪华 | Automatic medical cell separation device |
-
2011
- 2011-11-11 CN CN 201110356570 patent/CN102391946B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2585236Y (en) * | 2002-08-29 | 2003-11-05 | 江西特康科技有限公司 | Plunger-type syringe sealing structure of haemocytes analyzer |
| CN1833167A (en) * | 2003-05-30 | 2006-09-13 | 国家航天科技研究所“埃斯特万·特拉达斯” | Method and apparatus for the detection of substances or analytes from the analysis of one or more samples |
| CN101506643A (en) * | 2006-07-13 | 2009-08-12 | 海马生物科学公司 | Cell analysis apparatus and method |
| CN101797549A (en) * | 2009-02-11 | 2010-08-11 | 苏舍米克斯帕克有限公司 | Cartridge piston with exhaust apparatus |
| CN201368820Y (en) * | 2009-06-03 | 2009-12-23 | 武汉宏强医疗器械有限公司 | Injector for cell examination |
| CN201600378U (en) * | 2009-12-31 | 2010-10-06 | 南京劳拉电子有限公司 | Multichannel injector |
| CN102220224A (en) * | 2011-05-19 | 2011-10-19 | 汪华 | Automatic medical cell separation device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104931711A (en) * | 2015-06-25 | 2015-09-23 | 马鞍山海普微奇生物科技有限公司 | Method and device for promoting combination of biological reagents and biomolecules |
| CN108277152A (en) * | 2018-04-04 | 2018-07-13 | 徐俊 | The sterile liquid changing device of cell recovery and carry out the method that cell recovery changes liquid using the device |
| CN108277152B (en) * | 2018-04-04 | 2023-10-13 | 博品(上海)生物医药科技有限公司 | Sterile liquid exchange device for cell resuscitation and method for carrying out cell resuscitation liquid exchange by using device |
| CN109877152A (en) * | 2019-03-21 | 2019-06-14 | 贵州民族大学 | A kind of prosthetic device and restorative procedure for heavy metal ion in artificial swamp |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102391946B (en) | 2013-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9347033B2 (en) | Automated methods for isolating regenerative cells from adipose tissue | |
| CN105296327B (en) | A kind of nucleic acid-extracting apparatus and its extracting method | |
| CN102002461B (en) | IMB (immunomagnetic bead) based sequential fluid type fully-automatic magnetic separation device and method | |
| JP6913094B2 (en) | Cell Separator and How to Use It | |
| JP5998218B2 (en) | Magnetic body cover and nucleic acid extraction apparatus | |
| US9970953B2 (en) | Machine for automated extraction of nucleic acid | |
| CN105980550A (en) | Method and apparatus for processing and analyzing particles extracted from tangential filtering | |
| CN208964916U (en) | A kind of magnetic beads for purifying instrument of enrichment method | |
| CN102391946B (en) | Plunger sleeve and automatic cell processor therewith | |
| CN110407911A (en) | Magnetic beads for purifying system | |
| US11084035B2 (en) | Apparatus for cell preparation | |
| CN107760676A (en) | A kind of full-automatic extraction apparatus of paramagnetic particle | |
| CN202465700U (en) | Plunger sleeve and automatic cell treatment device comprising same | |
| CN109456880A (en) | Nucleic acid on-site rapidly extracting pipe and its application method | |
| US11833524B2 (en) | Combinatory separation | |
| CN102899247B (en) | Blood cell separation device | |
| CN201857395U (en) | Sequential flow type automatic magnetic sorting device based on immune magnetic bead | |
| CN208586235U (en) | Magnetic beads for purifying system | |
| US20150151221A1 (en) | Method and device for the concentration of multiple microorganisms and toxins from large liquid toxins | |
| WO2001038882A1 (en) | Apparatus and method for processing sample materials contained in a plurality of sample tubes | |
| CN111189789A (en) | Flow path system of full-automatic water anionic surfactant tester | |
| CN211785095U (en) | Flow path system of full-automatic water anionic surfactant tester | |
| CN209584204U (en) | A kind of use for laboratory nucleic acid extracting instrument | |
| CN214218610U (en) | Antibody purification equipment | |
| CN220626402U (en) | Full-automatic tester for anionic surfactant or volatile phenol and water quality analysis system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130123 Termination date: 20211111 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |