CN102389042A - Natural product formulation for increasing yield of rumen microbial proteins - Google Patents
Natural product formulation for increasing yield of rumen microbial proteins Download PDFInfo
- Publication number
- CN102389042A CN102389042A CN201110344762XA CN201110344762A CN102389042A CN 102389042 A CN102389042 A CN 102389042A CN 201110344762X A CN201110344762X A CN 201110344762XA CN 201110344762 A CN201110344762 A CN 201110344762A CN 102389042 A CN102389042 A CN 102389042A
- Authority
- CN
- China
- Prior art keywords
- policosanol
- natural products
- rumen
- balsamine
- natural
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to a method for preparing a natural product formulation which can promote growth of rumen microorganisms (particularly bacteria) and significantly increase the yield of rumen microbial proteins. Dry powders of garden balsam and an aqueous solution of natural policosanol are mixed proportionally to make a garden balsam-natural policosanol natural product formulation. Calculated on the basis of 0.2% to 0.8% additional amount, the natural product formulation can significantly promote the growth of rumen microorganisms (particularly bacteria), to increase the quantities of bacteria, fungi and protozoa by more than one order of magnitude on average, increase the yield of rumen microbial proteins by 49.12% on average and reduce the content of ammonia nitrogen by 40.78% on average; however, the natural product formulation has an inhibitory effect on rumen archaeobacteria and can reduce the quantity of rumen archaeobacteria by about one order of magnitude on average. The garden balsam-natural policosanol natural product formulation which is made from garden balsam dry powders and natural policosanol aqueous solution is an pure natural product formulation free of any artificial synthetic substance, and has broad application prospects in replacing urea to improve production performances of ruminants.
Description
Technical field:
The present invention relates to a kind of particularly growth of bacterium of rumen microorganism that promotes, significantly improve the preparation method of the natural products preparation of rumen microorganism protein yield.
Background technology:
Rumen microorganism has important role in the ruminant animal nutrition metabolism.At first as the structural of feed main body and unstructuredness carbohydrate; Have only through the rumen microorganism effect; Just can change into can be by the energy matter of ruminant using such as volatile fatty acid, and ruminant obtains 70%～80% of institute's energy requirement by this way.Secondly; Rumen microorganism can be transformed into high-quality microbial bacteria body protein to the low quality vegetable protein of searching for food; The mycoprotein that cud provides accounts for 40%～60% (Welch J G of ruminant desired protein; Hooper A P.Ingestion of feed and water [M] //Church D C.The ruminant animal digestive physiology and nutrition.Prospect Heights; Iuinois:Waveland Press, Inc, 1993:108-116.).In addition, also synthetic multivitamin of rumen microorganism and growth promoting substance are being brought into play important effect in assurance ruminant health and normal growth development.Therefore, promote the rumen microorganism growth, increase rumen mycoprotein output, significant for the production performance of improving ruminant.
Influence rumen microorganism growth and the synthetic principal element of mycoprotein environment (Clark J H such as (pH, osmotic pressure etc.) in feed nitrogenous source (peptide, amino acid, ammonia etc.), feed carbon source (structural and unstructuredness carbohydrate), mineral matter (constant and trace element), vitamin and the cud is arranged; Klusmeyer T H; Cameron M R.Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows [J] .J Dairy Sci; 1992,75:2304-2323; Hoover W H; Stokes S R.Balancing carbohydrates and proteins for optimum rumen microhial yield [J] .J Dairy Sci; 1991,74:3630-3644.), these influence factors are carried out reasonably optimizing; Can promote growth and mycoprotein synthetic of rumen microorganism to some extent, wherein increase the protein feeds intake and be effective method.Yet the shortage of protein feeds and costliness have greatly limited the practical application of this method, impel people's development and application nonprotein nitrogenous source feed.The nonprotein nitrogenous source feed of extensive use both at home and abroad is a urea at present.The nitrogen content of urea is 46%, 1kg urea be equivalent to 2.88kg thick protein (Zhao Yunhuan. improve the utilization rate [J] of ruminant domestic animal to urea. Xinyang agricultural higher junior college journal, 2000,10 (3): 12-14.).The rumen microorganism of ruminant can be converted into bacterium protein with nonprotein nitrogen in the urea; Under the situation of dietary protein deficiency; Additional urea can make milk production of cow improve 10%, make young ox weightening finish improve 24.3% (Wang Jinkuan. the control of ruminant poisoning with urea [J]. animal and veterinary impurity, 2010; 29 (1): 127,129.).Though urea can improve rumen mycoprotein output to a certain extent; But its palatability is poor; And hydrolysis rate is very fast asynchronous with the energy feed fermenting speed in cud; Cause the utilization rate of nitrogen not high (Zhang Mingfeng. U.S.'s forage grass and feed production production and processing industry development characteristic and trend [J]. grassland and lawn, 1994 (4): 15-19; Bloomfiled R A.Kinetics of urea metabolism in sheep.Joumal of Animal Science, 1960,19:1248.); The improper hyperammonemia that also can cause domestic animal of feeding; Cause acute poisoning, the death rate very high (Wang Jinkuan. the control of ruminant poisoning with urea [J]. animal and veterinary impurity, 2010; 29 (1): 127,129; Liu Zhang Zongsen gets first. the prophylactico-therapeutic measures of ruminant poisoning with urea [J]. and modern agriculture science and technology, 2008,7:184.), so urea is very limited in practical application as nonprotein nitrogen source feed.Exploitation can promote safe, the natural products efficiently that rumen microorganism growth and mycoprotein are synthetic, substitutes urea, helps to overcome the toxic and side effect and the metabolic deficiency of urea, improves the production performance of ruminant, the healthy aquaculture of propelling ruminant.
Summary of the invention:
Technical problem
The present invention is directed to the problems referred to above a kind of new natural products preparation is provided, add said preparation and can significantly promote the rumen microorganism growth, improve rumen microorganism mycoprotein output.
Technical scheme
Realize that technical scheme of the present invention is:
Gather the ripe plant branches and leaves of balsamine (Impatiens balsamina), put in 50 ℃ of drying boxes and dry to constant weight, pulverize the back and cross the 0.25mm sieve, process the balsamine plant drymeal.
Get the natural policosanol that contains triacontanol 65%, n-octacosanol 30%, the pure and mild hexacosanol 5% of lignocerane, being mixed with concentration is the policosanol aqueous solution of 10ppm.
In balsamine plant drymeal 1: the ratio of the policosanol aqueous solution 2 (g/mL); With the policosanol aqueous solution soaking of balsamine plant drymeal with 10ppm; Fully stir and process wet feed, put oven dry in 70 ℃ of drying boxes, go to 50 ℃ of baking ovens behind the crushing mixing and dry by the fire again; Until constant weight, process balsamine-policosanol natural products preparation.
Constant weight dry rice straw, corn and soya bean are pulverized, cross the 0.5mm sieve, be made into man-made feeds by 2: 1: 0.3 mixed.
In the adult ox body that permanent rumen fistula is housed, gather rumen fluid, four layers of filtered through gauze are processed the cud inoculation liquid in the anaerobic operation bag.
In 40 250mL blake bottles, inject 67mL cud inoculation liquid respectively, and add the 1.5g man-made feeds, with artificial saliva every bottle culture volume is transferred to 200mL again; Then 40 blake bottles are divided into 4 groups, 10 every group, by the certain proportion (g/mL) of culture volume; Divide into groups to add balsamine-policosanol natural products preparation; Addition is respectively 0,0.2%, 0.4% and 0.8%, and wherein 10 of 0 addition blake bottles are control group, and other is the test group of different additions.40 blake bottles that add honest material are used pure N
2The sealing of saturated back after fully mixing on 39 ℃ the shaking table, is inserted the 20mL syringe that is emptied completely again on the blake bottle seal cover, form the artificial rumen device, in 39 ℃ of constant incubators, cultivates.
Respectively 12,18,24h extracts fermentation broth sample, adopts the microprotein content in the purine method working sample, adopts the colorimetric method for determining ammonia nitrogen concentration; Extract the microbe genome DNA of each time point nutrient solution sample simultaneously, use the relative populations of RT-qPCR technology for detection bacterium, fungi, ancient bacterium and protozoon.
Beneficial effect
Microprotein and ammoniacal nitrogen detect and show; Add 0.2% balsamine-policosanol natural products preparation; Can make the microprotein output average of 12h, 18h and 24h improve 23.45%, 40.23% and 54.16% respectively, ammoniacal nitrogen is then average respectively to reduce by 30.12%, 36.37% and 46.68%; Add 0.4% balsamine-policosanol natural products preparation, make the microprotein output average of 12h, 18h and 24h improve 33.31%, 52.05% and 59.92% respectively, ammoniacal nitrogen respectively decreased average 27.13%, 36.61% and 41.39%; Add balsamine-policosanol natural products preparation of 0.8%, make the microprotein output average of identical time period improve 40.16%, 59.12% and 79.72% respectively, ammoniacal nitrogen is average respectively to descend 41.15%, 52.15% and 55.38%.
The RT-qPCR testing result shows, adds balsamine-policosanol natural products preparation and can significantly promote the rumen microorganism growth, compares with contrast, and rumen bacteria, fungi and protozoon quantity on average improve an about one magnitude, and wherein the effect of 0.8% addition is best; In contrast, balsamine-policosanol natural products preparation has obviously reduced the quantity of the ancient bacterium of cud.It is thus clear that balsamine-policosanol natural products preparation has certain inhibitory action to the ancient bacterium in the cud, and other microorganism in the cud is then had significant facilitation.
Balsamine is a kind of very easily traditional Chinese herbal medicine of cultivation, has ornamental value concurrently, the pure natural product that policosanol system adopts molecular distillation technique from beeswax, to extract.Balsamine-policosanol the preparation that utilizes balsamine plant drymeal and policosanol to process does not add any artificial synthetic, is a kind of pure natural product preparation, and the production performance that is used for replacing urea to improve ruminant will have broad application prospects.
The specific embodiment:
Gather the ripe plant branches and leaves of balsamine, put in 50 ℃ of drying boxes and dry to constant weight, pulverize the back and cross the 0.25mm sieve, process the balsamine plant drymeal.Get the natural policosanol that contains triacontanol 65%, n-octacosanol 30%, the pure and mild hexacosanol 5% of lignocerane, being mixed with concentration is the policosanol aqueous solution of 10ppm.In balsamine plant drymeal 1: the ratio of the policosanol aqueous solution 2 (g/mL); With the policosanol aqueous solution soaking of balsamine plant drymeal with 10ppm; Fully stir and process wet feed, put oven dry in 70 ℃ of drying boxes, go to 50 ℃ of baking ovens behind the crushing mixing and dry by the fire again; Until constant weight, process balsamine-policosanol natural products preparation.
Constant weight dry rice straw, corn and soya bean are pulverized, cross the 0.5mm sieve, be made into man-made feeds by 2: 1: 0.3 mixed.
In the adult ox body that permanent rumen fistula is housed, gather rumen fluid, in the anaerobic operation bag,, process the cud inoculation liquid with four layers of filtered through gauze.
In 40 250mL blake bottles, inject 67mL cud inoculation liquid respectively, and add the 1.5g man-made feeds, with artificial saliva every bottle nutrient solution volume is transferred to 200mL again; Then 40 blake bottles are divided into 4 groups, 10 every group, press the certain proportion of nutrient solution volume; Divide into groups to add balsamine-policosanol natural products preparation; Addition is respectively 0,0.2%, 0.4% and 0.8%, and wherein 10 of 0 addition blake bottles are control group, and other is the test group of different additions.40 blake bottles that add honest material are used pure CO
2Seal after driving away head space gas, after fully mixing on 39 ℃ the shaking table, on the blake bottle seal cover, insert the 20mL syringe that is emptied completely again, form the artificial rumen device, in 39 ℃ of constant incubators, cultivate.
Respectively 12,18,24h gathers fermentation broth sample, with the microprotein content in the purine method working sample, measure the result and see table 3; Use the colorimetric method for determining ammonia nitrogen concentration, measure the result and see table 4; Extract the microbe genome DNA of each time point nutrient solution sample simultaneously, use the relative populations of RT-qPCR technology for detection bacterium, fungi, ancient bacterium and protozoon, testing result is seen table 5.
The artificial saliva preparation:
Table 1 artificial saliva stoste preparation table
Table 2 artificial saliva preparation table (1000mL)
Table 3 balsamine-policosanol natural products preparation is to the influence of rumen microorganism protein output
Table 4 balsamine-policosanol natural products preparation is to the influence of cud nutrient solution ammoniacal nitrogen
Table 5 balsamine-policosanol natural products preparation is to the influence of rumen microorganism relative quantity
Claims (7)
1. natural products preparation that improves the rumen microorganism protein yield is characterized in that: with balsamine plant dry powder and the natural policosanol aqueous solution by a certain percentage, be mixed with balsamine-policosanol natural products preparation.
2. natural products preparation according to claim 1 is characterized in that:
Gather the ripe branches and leaves of balsamine, put in 50 ℃ of drying boxes and dry to constant weight, pulverize the back and cross the 0.25mm sieve, process the balsamine plant drymeal;
Get natural policosanol, being made into concentration is the policosanol aqueous solution of 10ppm;
In balsamine plant drymeal 1: the ratio of the policosanol aqueous solution 2 (g/mL), with the policosanol aqueous solution mixing of balsamine plant drymeal and 10ppm, 70 ℃～50 ℃ dry to constant weight, and process balsamine-policosanol natural products preparation.
3. according to claim 1 and 2 described natural products preparations, it is characterized in that: wherein natural policosanol contains triacontanol 65%, n-octacosanol 30%, the pure and mild hexacosanol 5% of lignocerane.
4. according to the described natural products preparation of claim 1～3, it is characterized in that: 0.2～0.8% (g/mL) that presses the cud culture volume adds the natural products preparation.
5. according to the described natural products preparation of claim 1～4, it is characterized in that: optimum addition is 0.8%.
6. according to the described natural products preparation of claim 1～5, it is characterized in that: the natural products preparation can significantly promote the particularly growth of bacterium of rumen microorganism, improves the rumen microorganism protein output, and reduces the quantity of ammonia nitrogen content and the ancient bacterium of cud.
7. according to the described natural products preparation of claim 1～6, it is characterized in that: said cud culture is a bovine rumen in vitro culture thing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110344762XA CN102389042A (en) | 2011-11-04 | 2011-11-04 | Natural product formulation for increasing yield of rumen microbial proteins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110344762XA CN102389042A (en) | 2011-11-04 | 2011-11-04 | Natural product formulation for increasing yield of rumen microbial proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102389042A true CN102389042A (en) | 2012-03-28 |
Family
ID=45856537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110344762XA Pending CN102389042A (en) | 2011-11-04 | 2011-11-04 | Natural product formulation for increasing yield of rumen microbial proteins |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102389042A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109864184A (en) * | 2019-04-03 | 2019-06-11 | 河北农业大学 | Cow feed capable of effectively inhibiting growth of rumen protozoa |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1660732A (en) * | 2004-12-31 | 2005-08-31 | 湖南太阳植物资源有限公司 | Technique for extracting and preparing policosanol and application |
| WO2009054737A1 (en) * | 2007-10-24 | 2009-04-30 | Methane Reduction Technologies Limited | Method of reducing greenhouse gas production |
| CN100553477C (en) * | 2006-02-14 | 2009-10-28 | 天津天隆农业科技有限公司 | A kind of pig starter feed and production method thereof that contains n-octacosanol |
-
2011
- 2011-11-04 CN CN201110344762XA patent/CN102389042A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1660732A (en) * | 2004-12-31 | 2005-08-31 | 湖南太阳植物资源有限公司 | Technique for extracting and preparing policosanol and application |
| CN100553477C (en) * | 2006-02-14 | 2009-10-28 | 天津天隆农业科技有限公司 | A kind of pig starter feed and production method thereof that contains n-octacosanol |
| WO2009054737A1 (en) * | 2007-10-24 | 2009-04-30 | Methane Reduction Technologies Limited | Method of reducing greenhouse gas production |
Non-Patent Citations (1)
| Title |
|---|
| 张志红，等: "几种植物提取物对瘤胃体外培养物甲烷生成的影响", 《江西科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109864184A (en) * | 2019-04-03 | 2019-06-11 | 河北农业大学 | Cow feed capable of effectively inhibiting growth of rumen protozoa |
| CN109864184B (en) * | 2019-04-03 | 2022-02-01 | 河北农业大学 | Cow feed capable of effectively inhibiting growth of rumen protozoa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101584375B (en) | Production method of chicken manure fermented feed | |
| CN101401616B (en) | Fermented abandoned vegetable feedstuff and preparation method thereof | |
| CN103300246B (en) | Feed for increasing milk of lactating sows and preparation method for feed | |
| CN103385360B (en) | Simple and efficient fermented soybean meal production technology and application thereof | |
| CN103385350B (en) | Soft particle milk replacer containing high fructose corn syrup and preparation thereof | |
| CN102178037B (en) | Method for producing feed of high-yield dairy cow by using corn straws fermented by Rhodotorula glutinis | |
| CN111011643A (en) | Bacterium enzyme fermented traditional Chinese medicine for improving growth performance of broiler chicken and preparation method thereof | |
| CN105795125A (en) | Preparation method of solid-state acidified and catabolic feed | |
| CN102604871A (en) | Probiotics fungicide for fermenting bean pulp and preparation method thereof | |
| CN108271938A (en) | A kind of functional seaweed fodder additive improving prevention of sow constipation | |
| CN101703106A (en) | Black locust flower yogurt and production method thereof | |
| CN106615640A (en) | Biologically fermented feed and preparation method thereof | |
| CN105543131A (en) | Compound bacteria/cottonseed meal fermented feed and preparation method thereof | |
| CN103141891A (en) | Preparation method of antrodia camphorata liver protection functional beverage | |
| CN104472882A (en) | Liquid biological feed additive | |
| CN109007295A (en) | A kind of Moringa feed addictive, preparation method and purposes | |
| CN108575885A (en) | A kind of black swine rearing method | |
| CN103960479B (en) | High protein orange residue fermented feed and preparation technology thereof | |
| CN103960463B (en) | Ensiling tangerine dregs of rice feed and preparation technology thereof | |
| CN106666112A (en) | Fattening feed for donkeys | |
| CN102352315A (en) | Microbial inoculum, preparation method thereof, and application of microbial inoculum to preparation of silage | |
| CN106721194B (en) | Additive for increasing beef intramuscular fat and flavor | |
| CN114190489B (en) | Preparation method of microbial fermentation chicken feed based on hybrid broussonetia papyrifera and mugwort mixture | |
| CN102389042A (en) | Natural product formulation for increasing yield of rumen microbial proteins | |
| CN103734497A (en) | Cow heat-stress resistant feed additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120328 |