CN102388861A - Stem cell storage tank with automatic liquid nitrogen filling control device - Google Patents
Stem cell storage tank with automatic liquid nitrogen filling control device Download PDFInfo
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- CN102388861A CN102388861A CN2011103016489A CN201110301648A CN102388861A CN 102388861 A CN102388861 A CN 102388861A CN 2011103016489 A CN2011103016489 A CN 2011103016489A CN 201110301648 A CN201110301648 A CN 201110301648A CN 102388861 A CN102388861 A CN 102388861A
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- liquid nitrogen
- stem cell
- storage tank
- pressure sensor
- cell storage
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- Filling Or Discharging Of Gas Storage Vessels (AREA)
Abstract
The invention discloses a stem cell storage tank with an automatic liquid nitrogen filling control device. The stem cell storage tank comprises an inner container and an outer container; a vacuum interlayer is formed between the inner container and the outer container; the bottom of the outer wall of the inner container is provided with a pressure sensor; the bottom of the external side of the stem cell storage tank is provided with a liquid nitrogen pipeline which is introduced into the inner container; the liquid nitrogen pipeline is provided with a solenoid valve which controls the opening and closing of the liquid nitrogen pipeline; the liquid nitrogen pipeline is externally connected with the liquid nitrogen tank; and the pressure sensor and the solenoid valve are connected with a controller which controls the opening and closing of the solenoid valve according to transmission data of the pressure sensor. The stem cell storage tank can be monitored in real time, and nitrogen can be filled into the storage tank according to the liquid level change of the liquid nitrogen and the preserved stem cells can be in a low-temperature state for a long time.
Description
Technical field
The present invention relates to a kind of stem cell holding vessel, especially a kind ofly have a stem cell holding vessel that liquid nitrogen fills control device automatically.
Background technology
The stem cell holding vessel is that stem cell stores utensil commonly used in the engineering; Existing stem cell holding vessel is made up of inner bag and outer courage; Form vacuum interlayer between inner bag and the outer courage, be used to keep low temperature, the stem cell that need store is put in the bottom of inner bag; In inner bag, be filled with liquid nitrogen, be used for the low-temperature preservation stem cell.
But the stem cell holding vessel of prior art because the loss of energy has part liquid nitrogen gasification to occur, reduces the liquid level of liquid nitrogen, influences the low-temperature storage of stem cell.
Summary of the invention
Technical problem to be solved by this invention is that a kind of stem cell holding vessel that liquid nitrogen fills control device automatically that has that can fill nitrogen automatically is provided.
In order to solve the problems of the technologies described above; The technical scheme that the present invention adopts is: a kind ofly have a stem cell holding vessel that liquid nitrogen fills control device automatically, comprise the inner bag and the outer courage that constitute holding vessel, form vacuum interlayer between inner bag and the outer courage; Outer wall bottom at inner bag is provided with pressure sensor; Stem cell holding vessel exterior lateral sides bottom is provided with the liquid nitrogen pipes that feeds in the inner bag, and liquid nitrogen pipes is provided with the magnetic valve that the control liquid nitrogen pipes opens and closes, the external liquid nitrogen container of liquid nitrogen pipes; Pressure sensor links to each other with controller with magnetic valve, and controller is according to the switching of the transmission Data Control magnetic valve of pressure sensor.
The invention has the beneficial effects as follows: can monitor in real time the stem cell holding vessel,, holding vessel in time filled nitrogen, guarantee that the stem cell of preservation is in low-temperature condition for a long time at any time according to the liquid nitrogen liquid level variation.
Description of drawings
Fig. 1 is the sketch map that liquid nitrogen fills the stem cell holding vessel of control device automatically that has of the present invention.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is done further explain:
The operation principle of stem cell holding vessel of the present invention is identical with prior art, can be with reference to prior art, so no longer narration, the difference of the present invention and prior art is to have increased liquid nitrogen to fill control device automatically, carries out detailed explanation below:
As shown in Figure 1; Of the present inventionly have a stem cell holding vessel that liquid nitrogen fills control device automatically, comprise the inner bag 1 and outer courage 2 that constitute holding vessel, form vacuum interlayer between inner bag 1 and the outer courage 2; Outer wall bottom at inner bag 1 is provided with pressure sensor 3; Stem cell holding vessel exterior lateral sides bottom is provided with the liquid nitrogen pipes 4 that feeds in the inner bag 1, and liquid nitrogen pipes 4 is provided with the magnetic valve 5 that the control liquid nitrogen pipes opens and closes, liquid nitrogen pipes 4 external liquid nitrogen container (not shown); Pressure sensor 3 links to each other with controller 6 with magnetic valve 5, and controller 6 is according to the switching of the transmission Data Control magnetic valve 5 of pressure sensor 3.
Specifically, the stem cell that needs are stored is placed into inner bag 1 bottom, charges into liquid nitrogen; Make stem cell be immersed in the liquid nitrogen fully; This moment, the height of liquid nitrogen was H1, opened liquid nitrogen and filled control device automatically, be i.e. pressure sensor 3, controller 6; The pressure of pressure sensor 3 impressions this moment is greater than the pressure P of setting of closing magnetic valve 1, and magnetic valve 5 cuts out.Because energy loss, the liquid nitrogen gasification in the holding vessel inner bag makes the liquid level of liquid nitrogen drop to H2; The pressure of pressure sensor 3 impressions this moment is less than the pressure P 2 of the opens solenoid valve of setting, and magnetic valve 5 is opened, and liquid nitrogen gets in the inner bag through liquid nitrogen pipes 4; When the liquid level of liquid nitrogen rises to H1; Be pressure sensor 3 impression pressure greater than set close the pressure P 1 of magnetic valve the time, control signal sends controller 6 to, controller 6 control magnetic valves 5 cut out; Repeat said process repeatedly, reach the purpose of filling nitrogen automatically.
In sum, content of the present invention is not confined in the above embodiments, and the knowledgeable people in the same area can propose other embodiment easily within technological guidance's thought of the present invention, but this embodiment is included within the scope of the present invention.
Claims (1)
1. one kind has the stem cell holding vessel that liquid nitrogen fills control device automatically; Comprise the inner bag (1) and the outer courage (2) that constitute holding vessel, form vacuum interlayer between inner bag (1) and the outer courage (2), it is characterized in that; Outer wall bottom at inner bag (1) is provided with pressure sensor (3); Stem cell holding vessel exterior lateral sides bottom is provided with the liquid nitrogen pipes (4) that feeds in the inner bag (1), and liquid nitrogen pipes (4) is provided with the magnetic valve (5) that the control liquid nitrogen pipes opens and closes, the external liquid nitrogen container of liquid nitrogen pipes (4); Pressure sensor (3) links to each other with controller (6) with magnetic valve (5), and controller (6) is according to the switching of the transmission Data Control magnetic valve (5) of pressure sensor (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110301648 CN102388861B (en) | 2011-10-09 | 2011-10-09 | Stem cell storage tank with automatic liquid nitrogen filling control device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110301648 CN102388861B (en) | 2011-10-09 | 2011-10-09 | Stem cell storage tank with automatic liquid nitrogen filling control device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102388861A true CN102388861A (en) | 2012-03-28 |
| CN102388861B CN102388861B (en) | 2013-04-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110301648 Active CN102388861B (en) | 2011-10-09 | 2011-10-09 | Stem cell storage tank with automatic liquid nitrogen filling control device |
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| CN (1) | CN102388861B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106081363A (en) * | 2016-06-06 | 2016-11-09 | 中国科学院合肥物质科学研究院 | A kind of liquid nitrogen zero is lost biological specimen low-temperature storing apparatus |
| CN110065665A (en) * | 2019-03-28 | 2019-07-30 | 淮南市知产创新技术研究有限公司 | A kind of reusable vacuum biological sample storage device and biological sample storage method |
| CN113197485A (en) * | 2021-06-09 | 2021-08-03 | 广东顺德高达科菲电器制造有限公司 | Controllable pressure extraction mechanism and extraction method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1858562A (en) * | 2006-05-25 | 2006-11-08 | 中国科学院等离子体物理研究所 | Buoyancy-type low temperature level meter |
| CN201643793U (en) * | 2009-12-04 | 2010-11-24 | 北京卫星环境工程研究所 | Gas-liquid separating system for liquid nitrogen |
| CN202286003U (en) * | 2011-10-09 | 2012-07-04 | 协和干细胞基因工程有限公司 | Stem cell storage pot with automatic liquid nitrogen filling control device |
-
2011
- 2011-10-09 CN CN 201110301648 patent/CN102388861B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1858562A (en) * | 2006-05-25 | 2006-11-08 | 中国科学院等离子体物理研究所 | Buoyancy-type low temperature level meter |
| CN201643793U (en) * | 2009-12-04 | 2010-11-24 | 北京卫星环境工程研究所 | Gas-liquid separating system for liquid nitrogen |
| CN202286003U (en) * | 2011-10-09 | 2012-07-04 | 协和干细胞基因工程有限公司 | Stem cell storage pot with automatic liquid nitrogen filling control device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106081363A (en) * | 2016-06-06 | 2016-11-09 | 中国科学院合肥物质科学研究院 | A kind of liquid nitrogen zero is lost biological specimen low-temperature storing apparatus |
| CN110065665A (en) * | 2019-03-28 | 2019-07-30 | 淮南市知产创新技术研究有限公司 | A kind of reusable vacuum biological sample storage device and biological sample storage method |
| CN110065665B (en) * | 2019-03-28 | 2021-03-09 | 淮南冠东信息科技有限公司 | Reusable vacuum biological sample storage device and biological sample storage method |
| CN113197485A (en) * | 2021-06-09 | 2021-08-03 | 广东顺德高达科菲电器制造有限公司 | Controllable pressure extraction mechanism and extraction method |
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| Publication number | Publication date |
|---|---|
| CN102388861B (en) | 2013-04-24 |
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