CN203776007U - Liquid nitrogen container for long-term storage of cells and biological materials - Google Patents
Liquid nitrogen container for long-term storage of cells and biological materials Download PDFInfo
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- CN203776007U CN203776007U CN201420159232.7U CN201420159232U CN203776007U CN 203776007 U CN203776007 U CN 203776007U CN 201420159232 U CN201420159232 U CN 201420159232U CN 203776007 U CN203776007 U CN 203776007U
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- liquid nitrogen
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Abstract
The utility model discloses a liquid nitrogen container for long-term storage of cells and biological materials. The liquid nitrogen container consists of a container body and a cover, wherein the container body has two layers and the middle interlayer is a vacuum layer; the cover is connected with the container body via a threaded opening; a safety air valve is arranged at the upper part of the outer side of the container body; a cryopreservation device and a liquid nitrogen storage device are arranged in the container body; the cryopreservation device and the liquid nitrogen storage device are arranged at intervals; and a liquid level alarm device is arranged at the lower part of the container body. According to the liquid nitrogen container for long-term storage of cells and biological materials, on one side, the volatilization of the liquid nitrogen can be decreased through a semi-enclosed storage manner; on the other side, the semi-enclosed stored liquid nitrogen can be added into the container body to complement the liquid nitrogen volume in the container body as required on the basis of the theory of communicating vessels, so that the service life of the liquid nitrogen is prolonged and the low-temperature storage effect of the samples such as important cell strains and tissues is strengthened.
Description
Technical field
The utility model relates to a kind of liquid nitrogen container for long-term cell preservation and biomaterial, belongs to frozen equipment technical field.Background technology
Liquid nitrogen container is mainly used in the biomaterial such as freeze-stored cell, tissue in laboratory, it is laboratory, hospital, local conventional a kind of preservation equipment such as factory, but because the design liquid nitrogen volatilization of traditional liquid nitrogen container is serious, need regular liquid nitrogen container liquid nitrogen is supplemented, in what is more important tank, the minimizing of amount of liquid nitrogen will affect the biologic activity to frozen article such as frozen vital tissue and cell lines greatly, this will cause great impact to the preservation of the frozen samples such as important cell line and use, even cannot use.The goal of the invention of this utility model is by existing tank body is improved, by adopting the mode of spiral mouth to increase the sealing of tank mouth, make full use of the inner space of liquid nitrogen container simultaneously, do not changing under the prerequisite of liquid nitrogen container volume size, in tank interior, add liquid nitrogen storage device part liquid nitrogen is carried out to semiclosed preservation, can reduce by semi-enclosed preserving type the volatilization of this part liquid nitrogen on the one hand, can when necessary, add tank interior to supplement tank body liquid nitrogen volume the liquid nitrogen of semiclosed preservation by law of connected vessels on the other hand, thereby extend liquid nitrogen service time, strengthen the sample low temperature preservation effects such as important cells strain and tissue.Utility model content
The utility model relates to a kind of liquid nitrogen container for long-term cell preservation and biomaterial, has solved traditional liquid nitrogen serious problem of volatilizing.
In order to address the above problem, the technical solution of the utility model is as follows: a kind of liquid nitrogen container for long-term cell preservation and biomaterial, by tank body and lid 1, formed, and lid 1 adopts the mode of spiral mouth to be connected with tank body; The outer upper of tank body is provided with safety air vavle 2, and tank interior is provided with cryopreservation device 10 and liquid nitrogen storage device 7, at least two of cryopreservation device 10 quantity, at least two of liquid nitrogen storage device 7 quantity, cryopreservation device 10 and liquid nitrogen storage device 7 intervals and put; The bottom of tank body is provided with liquid level warning device 5.
Above-mentioned tank body is double-deck, and intermediate course is vacuum layer 4.
Above-mentioned cryopreservation device 10 is connected with cryopreservation device handle 14 by axle, guarantees that cryopreservation device handle can rotate, and cryopreservation device handle 14 is connected with top cover 1 by line 13.
Above-mentioned liquid nitrogen storage device 7 bottoms are provided with liquid nitrogen and import and export 11.
Above-mentioned liquid nitrogen storage device 7 adopts law of connected vessels to be connected with tank interior.
Above-mentioned cryopreservation device (10) quantity is 4.
Above-mentioned liquid nitrogen storage device (7) quantity is 4.
The utility model can reduce by semi-enclosed preserving type the volatilization of this part liquid nitrogen on the one hand, can when necessary, add tank interior to supplement tank body liquid nitrogen volume the liquid nitrogen of semiclosed preservation by law of connected vessels on the other hand, thereby extend liquid nitrogen service time, strengthen the sample low temperature preservation effects such as important cells strain and tissue.
Accompanying drawing explanation
Fig. 1 is tank body front view;
(1, lid; 2, safety air vavle; 3, stopper; 4, vacuum layer; 5, liquid level warning device).
Fig. 2 is tank body vertical view;
(1, lid; 2, safety air vavle; 6, tank body handle).
Fig. 3 is tank interior vertical view;
(4, vacuum layer; 7, liquid nitrogen storage device; 8, tank body is outer; 9, tank body internal layer; 10, cryopreservation device).
Fig. 4 is liquid nitrogen storage device front view;
(2, safety air vavle, 7, liquid nitrogen storage device; 9, tank body internal layer; 11, liquid nitrogen is imported and exported).
Fig. 5 is liquid nitrogen storage device vertical view;
(9, tank body internal layer; 12, liquid nitrogen container storage facility dividing plate).
Fig. 6 is cryopreservation device schematic diagram;
(10, cryopreservation device; 13, line; 14, cryopreservation device handle).
Fig. 7 is that cryopreservation device tank body is placed front view:
(2, safety air vavle; 7, liquid nitrogen storage device; 10, cryopreservation device; 14, cryopreservation device handle).
Embodiment
Below in conjunction with Figure of description, the utility model is further explained.
For a liquid nitrogen container for long-term cell preservation and biomaterial, by tank body and lid 1, formed, lid 1 adopts the mode of spiral mouth to be connected with tank body; The outer upper of tank body is provided with safety air vavle 2, and tank interior is provided with cryopreservation device 10 and liquid nitrogen storage device 7, at least two of cryopreservation device 10 quantity, at least two of liquid nitrogen storage device 7 quantity, cryopreservation device 10 and liquid nitrogen storage device 7 intervals and put; The bottom of tank body is provided with liquid level warning device 5.
Described liquid nitrogen container tank body is double-deck, and intermediate course is vacuum layer 4, and to reach the object of isolated external temperature, the capping of tank mouth is that spiral mouth mode seals.At tank body lower part, load onto liquid level warning device 5 simultaneously, in the situation that being not enough to guarantee in tank Sample storage effect, liquid nitrogen liquid level reports to the police, to remind staff to supplement liquid nitrogen, tank structure is shown in Fig. 1 tank body front view, its and Fig. 2 tank body vertical view identical with left view.Advantage: the sealing means that changes traditional liquid nitrogen container, adopt spiral mouth to carry out the sealing effectiveness that liquid nitrogen container is strengthened in airtight preservation, intake valve adopts safety air vavle, and the function of intake valve and safety valve is united two into one, and utilizes liquid level warning device 5 prompting staff in time liquid nitrogen to be supplemented.
Tank interior mainly can be divided into Liang Ge great district, divides into greatly Si Ge community for two, and Liang Ge great district is respectively the frozen region of material and liquid nitrogen storage region, each great Qu all comprises Si Ge community, be respectively four same apparatus, to satisfy the demand, see Fig. 3 tank interior vertical view.Involved in the present invention to liquid nitrogen storage device 7 liquid nitrogen in traditional liquid nitrogen container is divided into two, a part of and traditional liquid nitrogen container is the same, the liquid level that is used for guaranteeing storage sample district liquid nitrogen is to preserve important cells strain and tissue sample, a part is by its semiclosed being saved in designed liquid nitrogen storage device 7 in addition, and main purpose is for reduce this part liquid nitrogen due to the volatilization of use and put procedure and liquid nitrogen in sample storage area supplemented simultaneously.Liquid nitrogen export and import is reserved in liquid nitrogen storage device 7 bottoms, in the time of both can guaranteeing to use, to the supplementing of liquid nitrogen in tank, can guarantee again in liquid nitrogen pouring process, the liquid nitrogen of liquid nitrogen storage device 7 to be supplemented.Liquid nitrogen storage device 7 adopts law of connected vessels to be connected with tank interior, when needs liquid nitrogen enters tank interior, can open the safe intake valve of liquid nitrogen storage device 7 tops, makes air slowly enter liquid nitrogen container tank interior, until two liquid levels are equal.Carrying out liquid nitrogen while supplementing, safe intake valve can be opened completely, liquid nitrogen is poured into liquid nitrogen storage device inner, filling after, close gas check valve, this part liquid nitrogen is carried out to semiclosed preservation.Four safety air vavles 2 that install on tank body top are managed respectively four semiclosed liquid nitrogen storage devices, and the liquid nitrogen that can simultaneously meet single and a plurality of liquid nitrogen storage devices 7 supplements, and sees front view and the vertical view of Fig. 4 and Fig. 5 liquid nitrogen storage device.Advantage: extend liquid nitrogen service time thereby part liquid nitrogen is carried out to semiclosed preservation, can supplement the liquid nitrogen in Sample storage region in tank body simultaneously, simple operations can be supplemented to tank interior by the liquid nitrogen of semiclosed preservation.
In tank interior, cryopreservation device 10 and liquid nitrogen storage device 7 intervals and put, as Fig. 6.Cryopreservation device 10 is connected with cryopreservation device handle 14 by axle, guarantee that cryopreservation device handle 14 can rotate, cryopreservation device handle 14 is connected with top cover 1 by line 13, at top, install can 360 degree rotations buckle, link for line 13, guarantees can not cause line 13 to twine together when screwing lid 1.After lid 1 covers, cryopreservation device handle 14 naturally droops, when lid 1 is opened, by line 13, mention cryopreservation device handle 14, thereby propose smoothly cryopreservation device 10, carry out the access of cell, see Fig. 6, cryopreservation device schematic diagram and Fig. 7 cryopreservation device tank body are placed front view.
Claims (7)
1. for a liquid nitrogen container for long-term cell preservation and biomaterial, it is characterized in that, tank body and lid (1), consist of, lid (1) adopts the mode of spiral mouth to be connected with tank body; The outer upper of tank body is provided with safety air vavle (2), tank interior is provided with cryopreservation device (10) and liquid nitrogen storage device (7), at least two of cryopreservation device (10) quantity, at least two of liquid nitrogen storage device (7) quantity, cryopreservation device (10) and liquid nitrogen storage device (7) interval and put; The bottom of tank body is provided with liquid level warning device (5).
2. liquid nitrogen container according to claim 1, is characterized in that, described tank body is double-deck, and intermediate course is vacuum layer (4).
3. liquid nitrogen container according to claim 1, is characterized in that, described cryopreservation device (10) is connected with cryopreservation device handle (14) by axle, and cryopreservation device handle (14) is connected with top cover (1) by line (13).
4. liquid nitrogen container according to claim 1, is characterized in that, described liquid nitrogen storage device (7) bottom is provided with liquid nitrogen and imports and exports (11).
5. liquid nitrogen container according to claim 1, is characterized in that, described liquid nitrogen storage device (7) adopts law of connected vessels to be connected with tank interior.
6. liquid nitrogen container according to claim 1, is characterized in that, described cryopreservation device (10) quantity is 4.
7. liquid nitrogen container according to claim 1, is characterized in that, described liquid nitrogen storage device (7) quantity is 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420159232.7U CN203776007U (en) | 2014-04-02 | 2014-04-02 | Liquid nitrogen container for long-term storage of cells and biological materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420159232.7U CN203776007U (en) | 2014-04-02 | 2014-04-02 | Liquid nitrogen container for long-term storage of cells and biological materials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203776007U true CN203776007U (en) | 2014-08-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420159232.7U Expired - Fee Related CN203776007U (en) | 2014-04-02 | 2014-04-02 | Liquid nitrogen container for long-term storage of cells and biological materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203776007U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977179A (en) * | 2015-05-12 | 2015-10-14 | 青岛农业大学 | Novel portable biological tissue low-temperature freezing sampling tank |
| CN107258766A (en) * | 2017-06-18 | 2017-10-20 | 广东博溪生物科技有限公司 | A kind of cell freezing method and cells frozen storing liquid |
| WO2023169239A1 (en) * | 2022-03-11 | 2023-09-14 | 上海原能细胞生物低温设备有限公司 | Storage device of biological sample low-temperature cryopreservation apparatus |
-
2014
- 2014-04-02 CN CN201420159232.7U patent/CN203776007U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977179A (en) * | 2015-05-12 | 2015-10-14 | 青岛农业大学 | Novel portable biological tissue low-temperature freezing sampling tank |
| CN107258766A (en) * | 2017-06-18 | 2017-10-20 | 广东博溪生物科技有限公司 | A kind of cell freezing method and cells frozen storing liquid |
| WO2023169239A1 (en) * | 2022-03-11 | 2023-09-14 | 上海原能细胞生物低温设备有限公司 | Storage device of biological sample low-temperature cryopreservation apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140820 Termination date: 20150402 |
|
| EXPY | Termination of patent right or utility model |