CN102382782A - Phytase microbial inoculum and preparation method thereof - Google Patents
Phytase microbial inoculum and preparation method thereof Download PDFInfo
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- CN102382782A CN102382782A CN2011103124619A CN201110312461A CN102382782A CN 102382782 A CN102382782 A CN 102382782A CN 2011103124619 A CN2011103124619 A CN 2011103124619A CN 201110312461 A CN201110312461 A CN 201110312461A CN 102382782 A CN102382782 A CN 102382782A
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Abstract
The invention relates to a phytase microbial inoculum which comprises a Bacillus Lip-3 strain and a fermentation culture medium, wherein the concentration of the Bacillus Lip-3 strain is 2-10*10<8>/mL; and the fermentation culture medium comprises the following components in percentage: 0.3-0.5% of beef extract, 0.5-1.5% of peptone and 0.4-0.6% of NaCl. The phytase microbial inoculum can promote the plant growth, increase the crop yield and improve the quality. The phytase microbial inoculum can be used for the cultivation of various vegetables, melons and fruits in agricultural greenhouses, such as cucumbers, tomatoes, pakchoi, kidney beans, aubergines, romaine lettuces, leaf lettuces, towel gourds, peppers, rape, sweet melons, strawberries, grapes and the like; the phytase microbial inoculum can be used for field crops, such as corn, wheat, soybeans, sugarcanes and the like; and the phytase microbial inoculum can be also used for fruit trees, such as peach trees, pear trees, jujube trees, apple trees and the like. Especially, the phytase microbial inoculum can promote the growth of Brassica chinensis L., can obviously increase the yield of cucumbers and tomatoes, and has considerable economic and social benefits.
Description
Technical field
The present invention relates to a kind of Sumizyme PHY microbial bacteria preparation.
Background technology
Increase beneficial microorganism quantity in the soil, can increase soil fertility, like works " Soil Fertilily " (U.S. Nova press, 2009: New York) said of people's public publications such as Liu Lili.The method that promotes soil fertility through increase soil beneficial microorganism quantity is a kind of method that develops the green ecological agricultural, is the method that China should greatly develop and help.Because China uses chemical fertilizer, country that agricultural chemicals quantity is maximum in the world.Extensively not only cause soil pollution to fertilize, also increased the weight of the eutrophication pollution of water body, even had influence on underground water and air.The superelevation amount is used the Soil structure that chemical fertilizer also destroys the arable land.Reduce the quality of agricultural-food, the disease and pest incidence improves.Simultaneously, the exhaustion of mineral resource has been quickened in the extraordinary development of chemical fertilizers production.Day by day become a big problem that threatens sustainable development of agricultural production.Correct lead farmers is rationally adjusted Fertilization Structure, the obvious free of contamination new-type fertilizer of development and popularization fertilizer efficiency, to realize the agriculture prodn increasing both production and income, to improve quality of agricultural product, recovery and raising soil Sustainable Production ability, is current agriculture prodn developing direction.
People such as Liu Lili were (screening study of Sumizyme PHY secretion bacterial strain, Tianjin Normal University journal, 26 (2): 19-22 in 2006; ISSN 1671-1114/CN 12-1337/N) public reported utilize be coated with flat band method from topsoil soils, filtered out 3 strains can secretion of phytase and diastatic genus bacillus (Bacillus) bacterial strain.Wherein Bacillus Lip-3 bacterial strain detects hydrolysis loop diameter and colony diameter ratio in the substratum at Sumizyme PHY, and promptly the value of D/d is the highest.Bacillus Lip-3 bacterial strain tieback soil has lifting soil fertility, for growth and development of plants provides phosphorus nutrition, improves the effect of plant biomass.
Summary of the invention:
A kind of Sumizyme PHY microbial bacteria preparation and preparation method have been the object of the present invention is to provide.The present invention can promote plant-growth and the output that improves crop, has considerable economic and social benefit.
A kind of Sumizyme PHY microbial bacteria preparation provided by the invention comprises Bacillus Lip-3 bacterial strain, fermention medium, and the concentration of Bacillus Lip-3 bacterium is 2～10x10
8Individual/mL.
Each composition proportion of fermention medium is: Carnis Bovis seu Bubali cream 0.3～0.5%, and peptone 0.5～1.5%, NaCl 0.4～0.6%.
The preparation method of a kind of Sumizyme PHY microbial bacteria preparation provided by the invention comprises the steps:
1) from consisting of: picking one connects the liquid nutrient medium (Carnis Bovis seu Bubali cream 0.3% that collarium Bacillus Lip-3 thalline inserts 50ml on the bacterial classification of the slant medium of Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaCl 0.5%, agar powder 1.5% and pH 7.0～7.2; Peptone 1%; NaCl 0.5%; PH 7.0～7.2) in, 12h is cultivated in 37 ℃ of concussions, is seed liquor.Can use this method to continue to enlarge seed liquor.
2) with 6～10% inoculum size seed liquor is inserted in the fermention medium,, obtain the Sumizyme PHY microbiobacterial agent in 30～37 ℃ of fermentor tank aerobic culture 2～6d.Each composition proportion of fermention medium is: Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaCl0.5%.Substratum is formed: Carnis Bovis seu Bubali cream 0.3%, and peptone 1%, NaCl 0.5%, and pH 7.0～7.2.The concentration of Bacillus Lip-3: 2～10x10
8Individual bacterium/mL.
The Sumizyme PHY bio-feritlizer that described Sumizyme PHY microbial bacteria preparation and brid guano, agricultural crop straw are processed can directly be applied to farm crop.
The invention provides a kind of Sumizyme PHY microbial bacteria preparation as plant-growth regulator, can promote plant-growth and improve crop yield, Sumizyme PHY microbial bacteria preparation can obviously promote growing of plant, improves quality.Sumizyme PHY microbial bacteria preparation is used for the cultivation of the various vegetable melon and fruits of agricultural greenhouse, like cucumber, tomato, Plantula Brassicae chinensis, fresh kidney beans, eggplant, romaine lettuce, naked oats dish, sponge gourd, capsicum, rape, muskmelon, strawberry, grape etc.; Be used for field crop, like corn, wheat, soybean, sugarcane etc.; Be used for fruit tree, like peach, pear tree, jujube tree, apple tree etc.Particularly the blue or green growth in Shanghai there is promoter action and cucumber, tomato are had significant effect of increasing production, have considerable economic and social benefit.
Description of drawings
The mensuration result of the blue or green plant phosphorus content in Fig. 1 Shanghai.
The mensuration result of the blue or green plant nitrogen content in Fig. 2 Shanghai.
Embodiment:
Below in conjunction with embodiment the present invention is done further explanation, it is indicative that embodiment is merely, and means that never it limits scope of the present invention by any way.
The preparation method one of embodiment 1 Sumizyme PHY microbial bacteria preparation
From slant medium (NaCl 0.5% for Carnis Bovis seu Bubali cream 0.3%, peptone 1%, agar powder 1.5%, pH 7.0～7.2.) bacterial classification on picking one connect collarium Bacillus Lip-3 thalline (by this experiment screening and preserve, can sell) and insert 50ml liquid nutrient medium (Carnis Bovis seu Bubali cream 0.3%, peptone 1%; NaCl 0.5%; PH 7.0～7.2) in, 12h is cultivated in 37 ℃ of concussions, is seed liquor.500ml triangular flask adding 300ml substratum (Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaC chamber 10.5%, pH 7.0～7.2), the inoculum size with 10% inserts seed liquor in the fermention medium, cultivates in 37 ℃ of concussions.Obtain the Sumizyme PHY microbiobacterial agent.
The preparation method two of embodiment 2 Sumizyme PHY microbial bacteria preparations
Inoculum size with 10% inserts the seed liquor of Bacillus Lip-3 bacterium in the fermention medium, in 30 ℃ of fermentor tank aerobic culture 4d.Obtain the Sumizyme PHY microbiobacterial agent.
The fertilizer of embodiment 3 Sumizyme PHY microbial bacteria preparations
The Bacillus Lip-3 thalline of getting the fermentor tank aerobic culture mixes with chicken manure, crop material.Blending ratio: Bacillus Lip-3 thalline fermented liquid 10%, chicken manure 50%, crop material 40%.Solid fermentation 30d.Obtain the Sumizyme PHY bio-feritlizer.
The fertilizer of embodiment 4 Sumizyme PHY microbial bacteria preparations
The Bacillus Lip-3 thalline of getting the fermentor tank aerobic culture mixes with pig manure, crop material.Blending ratio: Bacillus Lip-3 thalline fermented liquid 10%, pig manure 50%, crop material 40%.Solid fermentation 30d.Obtain the Sumizyme PHY bio-feritlizer.
The fertilizer of embodiment 5 Sumizyme PHY microbial bacteria preparations
The Bacillus Lip-3 thalline of getting the fermentor tank aerobic culture mixes with pig manure, chicken manure, crop material.Blending ratio: Bacillus Lip-3 thalline fermented liquid 10%, pig manure 30%, chicken manure 30%, crop material 30%.Solid fermentation 30d.Obtain the Sumizyme PHY bio-feritlizer.
Embodiment 6 Sumizyme PHY microbial bacteria preparations are to the influence of Shanghai young crops phase growth
1) embodiment material and method
(1) supplies examination soil
Supply examination soil to pick up from Tianjin Normal University biotechnology garden, its characteristic is following.
Soil type: saline moist soil; PH:7.6; Full nitrogen (g/kg): 0.94; Rapid available phosphorus (mg/kg): 5.30; Available potassium (mg/kg): 106; Organic (%): 2.94; Water ratio (%): 8.87.
(2) plant seed
The blue or green formal name used at school in Shanghai is: Brassica chinensis L..Trade(brand)name is: Shanghai is blue or green.Seed is purchased the kind industry institute in the autumn fields, Nanjing.
(3) Sumizyme PHY microbial bacteria preparation (obtaining) with embodiment method one
(4) mensuration of the blue or green plant chlorophyll content in Shanghai
The acetone extraction method.Operation steps referring to document (1. Song Ye army, Ji Chongguang. Modern Analytical Instrument and testing method [M]. Xi'an: press of Northwest University, 1994. 2. Wu Yue swallows; Wang's wealth is given birth to; Wu Qiufeng. some photosynthetic physiological characteristics [J] of grape in the overcast and rainy environment. gardening journal, 2005,32 (32): 387-391.).
(5) mensuration of the blue or green plant total nitrogen content in Shanghai
Adopt nitrogen determination, measure with kjeldahl apparatus.Plant and instrument: bright sound 20 hole graphite module automatic slaking apparatus S20A types and bright sound full-automatic azotometer K06C type.
(6) mensuration of the blue or green plant content of tatal phosphorus in Shanghai
Operation steps is referring to Zhang Zhiliang. and plant physiology experiment instructs (the 4th edition), Higher Education Publishing House, ISBN:9787040272765,2010.
(7) experimental design
Soil is pressed 10kg/ basin packing (the high 26.0cm of basin, back cut diameter are 25.0cm, and lower port diameter is 22.0cm).20 of every basin sowings; Thinning to the 10 strain/basin behind the 7d of emerging, Sumizyme PHY microbiobacterial agent impose on 10～20cm place under the seed, and Sumizyme PHY microbiobacterial agent consumption is a 300kg/ mu; Not applying any microbial inoculum or fertilizer or chemical fertilizer is blank, and each is handled 7 times and repeats.Water at regular time and quantity.Gather in the crops behind the potted plant cultivation 30d, measure plant height respectively, fresh weight, living weight, chlorophyll content, plant nitrogen content, plant phosphorus content.
2) result and analysis
(1) the Sumizyme PHY microbiobacterial agent is to the influence of the blue or green growth in Shanghai
Gather in the crops behind the blue or green cultivation in the Shanghai 30d, measure plant height respectively, fresh weight, living weight is seen table 1.The result shows that the Sumizyme PHY microbiobacterial agent all has certain promoter action to the blue or green plant height in Shanghai, ground fresh weight, underground fresh weight, ground biomass, underground biomass, but degree is different.Aspect plant height, the blue or green average plant height in the Shanghai that the Sumizyme PHY microbiobacterial agent is handled is 15.33cm, is significantly higher than blank; Aspect the blue or green over-ground part fresh weight in Shanghai, the fresh weight of going up fifty-fifty that the Sumizyme PHY microbiobacterial agent is handled is 3.54g, is significantly higher than blank; Aspect the blue or green underground part fresh weight in Shanghai, the underground fresh weight that the Sumizyme PHY microbiobacterial agent is handled is 0.88g to the maximum, is significantly higher than blank; Living weight aspect on the ground, the blue or green ground biomass in Shanghai is 0.583g, is significantly higher than blank; Aspect underground biomass, the Shanghai green grass or young crops that the Sumizyme PHY microbiobacterial agent is handled also is significantly higher than blank.This shows that the Sumizyme PHY microbiobacterial agent has certain promoter action to the blue or green growth in Shanghai.
Table 1 Sumizyme PHY microbiobacterial agent is to the result that influences of the blue or green growth in Shanghai
(2) the Sumizyme PHY microbiobacterial agent is to the influence of the green cellulose content of Shanghai leafiness
Measure the blue or green chlorophyll a in Shanghai, chlorophyll b and total chlorophyllous content respectively at 15d and 30d, the result sees table 2.Though the difference of leaf green content is very not remarkable, each chlorophyll content of handling is all a little more than blank.
The mensuration result of the green cellulose content of table 2 Shanghai leafiness
(4) the Sumizyme PHY microbiobacterial agent is to the influence of the blue or green phosphorus content in Shanghai
Phosphorus has almost been participated in whole building-up reactionss of cell, and in energy transfer, transmission and photosynthesis, has been played an important role as one of three essential big nutritive element of growth and development of plants, and nitrogen metabolism is also had very big influence.The phosphorus element can promote the absorption and the assimilation of nitrogen, and phosphorus is the important component part of nitrate reductase and nitrite reductase, participates in NO
3 -Reduction and assimilation; Simultaneously, phosphorus is the energy source of growth and development of plants, in nutrient balance, plays an important role.In time supply with the phosphorus nutrient, can impel the various metabolic processes of crop to carry out smoothly, make the plant substance in vivo synthetic and decompose, move and accumulation is able to harmoniously, reach raising output, improve the purpose of quality.
Fig. 1 shows that the Sumizyme PHY microbiobacterial agent can increase the intravital phosphorus content of plant significantly, and phosphorus content is 0.68%, is significantly higher than blank.
(5) the Sumizyme PHY microbiobacterial agent is to the influence of the blue or green nitrogen content in Shanghai
Nitrogen is the important component part of nitrogenous compound in the cell, and they occupy special role in vital movement.Therefore, nitrogen is called as the element of life.The formation of enzyme and many coenzyme and prothetic group also all has nitrogen to participate in.Nitrogen still is the composition of certain plants hormone, VITAMINs, and they play important regulatory role to vital movement.In addition, nitrogen is chlorophyllous composition, with photosynthesis substantial connection is arranged, so the whether abundant quality that reaches plant nitrogen nutrition of nitrogen supply affects the formation of growth and development of plants and output and the height of quality to a great extent.
Fig. 2 shows that the Shanghai intravital nitrogen content of blue or green plant that applies the Sumizyme PHY microbiobacterial agent significantly increases, and its nitrogen content is 2.72%, is significantly higher than blank.
The cultivation of embodiment 7 cucumber
Implement the place: the newly-built greenhouse of the blue or green Hou Cun of coagulating in Xiqing District big temple town.
Soil type: the slight saline moist soil of middle loamy texture muriate vitriol.Organic 26.9g/kg, alkali-hydrolyzable nitrogen 174.9mg/kg, rapid available phosphorus 4.7mg/kg, available potassium 195.3mg/kg, pH 8.6.
Implement crop: cucumber, kind are Tianjin spring No. 2.Field planting on February 19, June 27 uprooted plants after their edible portions have been harvested.
Test is handled: handle 1, routine is used chemical fertilizer; Handle 2, routine is used chemical fertilizer+3000 kilogram chicken manure; Handle 3, routine is used chemical fertilizer+3000 kilogram chicken manure+60 kilograms of Sumizyme PHY bio-feritlizers.Conventional fertilizer application: base fertilizer is fertile (N16-N16-N18) 50kg/ mu of compound; Topdress to execute urea 10kg/ mu, fertile (N16-P16-K18) 10kg/ mu of compound at every turn, impose altogether 7 times.
Result of implementation and analysis:
(1) growing state
The cucumber leaves of using the Sumizyme PHY bio-feritlizer is dark green, and fast growth was bloomed 2～3 days in advance, and cucumber is dark green and have gloss, and sugar-preserved gourd is straight, mouthfeel delicate fragrance.
(2) yield result
Table 1 output situation (kilogram/mu)
Table 2 variance analysis
| Source of variation | Degree of freedom | Sum of squares | All square | The F value | F0.05 | F0.01 |
| Between processing | 2 | 666694.88 | 333347.44 | 48.945 | 6.944 | 18.000 |
| Between |
2 | 79158.22 | 39579.11 | 5.811 | 6.944 | 18.000 |
| |
4 | 27242.44 | 6810.61 | - | - | - |
| Total variation | 8 | 773095.55 | - | - | - | - |
It is remarkable that ANOVA showed significant is handled differences, handles a multiple comparisons analysis.
Table 3 standard of comparison
| Test the average of extreme difference | 5％ | 1％ |
| Several numbers (P) | SSR | | SSR | LSR | |
| 2 | 3.930 | 187.251 | 6.510 | 310.180 | |
| 3 | 4.010 | 191.063 | 6.800 | 323.997 |
Table 4 is respectively handled rate ratio
Therefore, on cucumber produces, use the growth that the Sumizyme PHY bio-feritlizer can obviously promote plant, improve quality, improve, have significant effect of increasing production than using common fertilizer output.
The cultivation of embodiment 8 tomatoes
Implement the place: newly-built canopy chamber, Er Houzhuan village, town, the steady village of Xiqing District king, canopy chamber specification: 80 * 7m, every furrow 6.5 * 1.2m.
Soil type: the slight saline moist soil of middle loamy texture muriate vitriol.Organic 15.11g/kg, alkali-hydrolyzable nitrogen 80.68mg/kg, rapid available phosphorus 7.92mg/kg, available potassium 265.3mg/kg, pH8.3.
The enforcement crop is a tomato, and kind is No. 2, good powder.
Test is handled: handle 1, routine is used chemical fertilizer; Handle 2, routine is used chemical fertilizer+800 kilogram organic commercial fertilizer; Handle 3, routine is used chemical fertilizer+800 kilogram organic commercial fertilizer+40 kilograms of Sumizyme PHY bio-feritlizers.Conventional fertilizer application: base fertilizer is fertile (N17-P17-K17) 75kg/ mu of compound; Topdress to execute urea 15kg/ mu at every turn, impose altogether 3 times.
Result of implementation and analysis
(1) different treatment is to the influence of tomato biological character
Through the field test investigation, the sub-district of using the Sumizyme PHY bio-feritlizer, plant strain growth is vigorous, well developed root system, robust plant, blade is plump, and the leaf look dark green; Invalid flower and malformed fruit reduce, and the disease resistance ability strengthens; Fruit shape is big and neat, color and luster is vivid, and is in advance ripe.
(2) different treatment is to the influence of tomato production, and each is handled output field investigation situation and sees the following form:
Table 1 yield trials is unit as a result: kg/ mu
Table 2 variance analysis
| Become because of | Degree of freedom | Sum of squares | All square | The F value | F 0.05 | F 0.01 |
| Between district's |
2 | 547280.889 | 273640.444 | 88.420 | 6.944 | 18.000 |
| Between processing | 2 | 27664.222 | 13832.111 | 4.470 | 6.944 | 18.000 |
| |
4 | 12379.111 | 3094.778 | - | - | - |
| Total variation | 8 | 587324.222 | - | - | - | - |
Minimum significantly range method (LSR-SSR): error degree of freedom: 4, the mean size of a sample: 3, mean standard error: 32.118.
Table 3 multiple comparisons standard
Table 4 multiple comparisons
Above data analysis shows that it is extremely remarkable to handle differences, and district's group difference is not obvious.Handling 3 ratio processing 2 increases production 7.57%, increases production 16.05% than handling 1; Handle 2 than handling 1 raising the output 7.88%.
Therefore, the Sumizyme PHY bio-feritlizer can obviously promote growing of plant, improves quality, and tomato is had significant effect of increasing production.
Claims (9)
1. a Sumizyme PHY microbial bacteria preparation is characterized in that it comprises Bacillus Lip-3 bacterial strain, fermention medium, and the concentration of Bacillus Lip-3 bacterium is 2～10x10
8Individual/mL.
2. according to the described Sumizyme PHY microbial bacteria of claim 1 preparation, it is characterized in that each composition proportion of described fermention medium is: Carnis Bovis seu Bubali cream 0.3～0.5%, peptone 0.5～1.5%, NaCl 0.4～0.6%.
3. the preparation method of the described Sumizyme PHY microbial bacteria of claim 1 preparation is characterized in that it comprises the steps:
1) from consisting of: picking one connects the liquid nutrient medium of collarium Bacillus Lip-3 thalline access 50ml on the bacterial classification of the slant medium of Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaCl 0.5%, agar powder 1.5% and pH 7.0～7.2; 12h is cultivated in 37 ℃ of concussions, is seed liquor;
2) with 6～10% inoculum size seed liquor is inserted in the fermention medium,, obtain the Sumizyme PHY microbiobacterial agent in 30～37 ℃ of fermentor tank aerobic culture 2～6d.
4. according to the described method of claim 3, it is characterized in that each composition proportion of described fermention medium is: Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaCl 0.5%.
5. according to the described method of claim 3, it is characterized in that described liquid nutrient medium composition: Carnis Bovis seu Bubali cream 0.3%, peptone 1%, NaCl 0.5%, and pH 7.0～7.2.
6. according to the described method of claim 3, it is characterized in that the concentration of BacillusLip-3 in the Sumizyme PHY microbiobacterial agent of described acquisition: 2～10x10
8Individual bacterium/mL.
7. the Sumizyme PHY bio-feritlizer processed of claim 1 a described Sumizyme PHY microbial bacteria preparation and animal dung, agricultural crop straw.
8. the application of the described Sumizyme PHY microbial bacteria of claim 1 preparation is characterized in that the regulator as plant-growth.
9. according to the described application of claim 8, it is characterized in that regulator as Shanghai green grass or young crops, cucumber, tomato plant-growth.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103833427A (en) * | 2013-11-07 | 2014-06-04 | 天津师范大学 | Water-soluble biological fertilizer |
| CN104761299A (en) * | 2015-03-17 | 2015-07-08 | 山东农业大学 | Phytase organic fertilizer and preparation method thereof |
| CN106577772A (en) * | 2016-12-02 | 2017-04-26 | 青岛蔚蓝生物集团有限公司 | Novel biological seed coating and application thereof |
| CN107365218A (en) * | 2017-08-31 | 2017-11-21 | 天津绿之本生物科技有限公司 | A kind of biological organic fertilizer with insecticidal function and preparation method thereof |
| CN110669694A (en) * | 2019-10-25 | 2020-01-10 | 湖北华扬科技发展有限公司 | Bacillus histolyticus phosphorus reduction culture medium, fermentation method and application |
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| CN101195543A (en) * | 2007-12-18 | 2008-06-11 | 天津师范大学 | Phytase microbial inoculum fertilizer, producing method and application thereof |
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| CN101195543A (en) * | 2007-12-18 | 2008-06-11 | 天津师范大学 | Phytase microbial inoculum fertilizer, producing method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103833427A (en) * | 2013-11-07 | 2014-06-04 | 天津师范大学 | Water-soluble biological fertilizer |
| CN103833427B (en) * | 2013-11-07 | 2015-08-19 | 天津师范大学 | A kind of water-soluble bio-feritlizer |
| CN104761299A (en) * | 2015-03-17 | 2015-07-08 | 山东农业大学 | Phytase organic fertilizer and preparation method thereof |
| CN106577772A (en) * | 2016-12-02 | 2017-04-26 | 青岛蔚蓝生物集团有限公司 | Novel biological seed coating and application thereof |
| CN106577772B (en) * | 2016-12-02 | 2020-04-14 | 青岛蔚蓝生物集团有限公司 | Biological seed coating agent and application thereof |
| CN107365218A (en) * | 2017-08-31 | 2017-11-21 | 天津绿之本生物科技有限公司 | A kind of biological organic fertilizer with insecticidal function and preparation method thereof |
| CN110669694A (en) * | 2019-10-25 | 2020-01-10 | 湖北华扬科技发展有限公司 | Bacillus histolyticus phosphorus reduction culture medium, fermentation method and application |
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Application publication date: 20120321 |