CN102349543A - Application of calcined shell/nanometer Cu2O composite - Google Patents
Application of calcined shell/nanometer Cu2O composite Download PDFInfo
- Publication number
- CN102349543A CN102349543A CN2011102009581A CN201110200958A CN102349543A CN 102349543 A CN102349543 A CN 102349543A CN 2011102009581 A CN2011102009581 A CN 2011102009581A CN 201110200958 A CN201110200958 A CN 201110200958A CN 102349543 A CN102349543 A CN 102349543A
- Authority
- CN
- China
- Prior art keywords
- composite
- nanometer
- shell
- application
- aeromonas hydrophila
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Physical Water Treatments (AREA)
Abstract
The invention relates to application of a nanocomposite in inhibiting pathogens, and specifically to application of a calcined shell/nanometer Cu2O composite. The calcined shell/nanometer Cu2O composite has a bactericidal effect. The calcined shell/nanometer Cu2O composite employed in the invention has good bacteriostasis and bactericidal effects and can powerfully kill aeromonas hydrophila and bibrio parahemolyticus respectively in ultraviolet light and sunlight.
Description
Technical field
The present invention relates to utilize nano composite material to suppress pathogen, a kind of specifically calcining shell/nanometer Cu
2The application of O composite.
Background technology
Aeromonas hydrophila (Aeromonas hydrophila) is the pathogen of bacteremic septicemia, can cause the generation of various freshwater fish septicemia.It is acute popular that bacteremic septicemia is more, and morbidity back lethality is high, already causes heavy economic loss often for freshwater fish culturing.Vibrio parahaemolytious (Bibrio parahemolyticus) is the halophagia Gram-negative bacteria, is distributed in bay, zone, coastline, salt lake and the marine product, and be one of pathogenic bacteria in ocean water body and the marine organisms.These two kinds of germs not only endanger aquatile itself, also can cause that the people poisons by food through food chain, diarrhoea etc.To the control of these two kinds of pathogens, be main mainly at present, but be prone to produce problems such as drug resistance, medicament residue with antibiotic and chemicals, therefore, need to seek the sterilizing technology of a kind of efficient more, environmental protection, safety.
Summary of the invention
The object of the invention provides a kind of calcining shell/nanometer Cu
2The application of O composite.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is:
A kind of calcining shell/nanometer Cu
2The application of O composite: calcining shell/nanometer Cu
2The O composite has bactericidal action.
Said calcining shell/nanometer Cu
2The O composite can be used as the bactericide that suppresses Aeromonas hydrophila or vibrio parahaemolytious.Said calcining shell/nanometer Cu
2The preparation of O composite is the conch meal that in the little reaction system of the Thermodynamically stable of being made up of NaCl and neopelex, adds CuCl and high-temperature activation; Continue to stir, utilize the flourishing hole of activation conch meal and basicity to make the cuprous salt hydrolysis and closely be carried on the conch meal surface; Through Separation of Solid and Liquid, ultrasonic washing, distilled water flushing, dehydrate after, synthetic shell/nanometer Cu
2The O composite.
Principle of the present invention: calcining shell/nanometer Cu
2Nanometer Cu in the O composite
2After O receives the optical excitation of wavelength≤600nm, nanometer Cu
2O decomposes the electronegative electronics (e that generation moves freely voluntarily in water and air
-), stay the hole (h of positively charged simultaneously
+):
Cu
2O+hv→Cu
2O+e
-+h
+
e
-+ h
+→ surface recombination+Q (heat)
Then, the oxygen in the water becomes active oxygen as the electron capture agent behind its trapped electron, with the H in the solution
+Reaction generates HO successively
2And HO, hole (h
+) and H
2O combines also can produce HO; Large biological molecule such as effects such as lipid, protein, enzyme and nucleic acid molecule in the microbial cells such as these free radicals and bacterium, virus; Destroy cell structure through a series of chain type oxidation reactions; Make protein of bacteria variation, lipolysis, make bacterium cause death, thereby play the effect of sterilizing:
H
2O→H
++OH
-
O
2 -+2HO
2·→·OH+OH
-+2O
2
h
++H
2O→·OH+H
+
R (large biological molecule)+OH → CO
2+ H
2Other products of O+
At last, electronics (e
-) and hole (h
+) buried in oblivion reaction terminating:
4e
-+O
2+4H
+→2H
2O
R+h
+→ CO
2+ H
2Other products of O+.
The advantage that the present invention had:
Calcining shell/nanometer Cu that the present invention adopts
2The O composite has good restraining and sterilizing bacteria effect, under ultraviolet light and sunshine, Aeromonas hydrophila and vibrio parahaemolytious is all had the very strong ability of killing respectively.When composite concentration was 100mg/L, irradiation 2h can reach more than 98% these two kinds of kill pathogenic bacteria rates under ultraviolet light, and irradiation 2h killing rate all can reach more than 96% under sunshine.Experiment showed, the composite good stability that adopts among the present invention, can repeatedly use repeatedly, solved problems such as drug resistance that traditional bactericide brings, medicament residue, is a kind of sterilization technology than more efficient, environmental protection, safety.
Description of drawings
Fig. 1 a be the composite that provides of the embodiment of the invention under ultraviolet light, along with the variation of composite dosage its to two kinds of kill pathogenic bacteria design sketch.
Fig. 1 b be the composite that provides of inventive embodiments under sunshine, along with the variation of composite dosage its to two kinds of kill pathogenic bacteria design sketch.
Fig. 2 a be the composite that provides of the embodiment of the invention under ultraviolet light, it is to two kinds of kill pathogenic bacteria design sketch over time.
Fig. 2 b be the composite that provides of inventive embodiments under sunshine, along with change of time its to two kinds of kill pathogenic bacteria design sketch.
Embodiment
Embodiment 1
Calcining shell/nanometer Cu
2The preparation of O composite: the conch meal that in the little reaction system of the Thermodynamically stable of forming by NaCl and neopelex, adds CuCl and high-temperature activation; Continue to stir, utilize the flourishing hole of activation conch meal and basicity to make the cuprous salt hydrolysis and closely be carried on the conch meal surface; Through Separation of Solid and Liquid, ultrasonic washing, distilled water flushing, dehydrate after, synthetic shell/nanometer Cu
2The O composite wood.
Specifically prepare referring on 09 11st, 2010 applying date application number 201010281703.8, denomination of invention: discarded shell loaded with nano Cu
2O composite photocatalyst material and preparation thereof and application.
Calcining shell/nanometer Cu with above-mentioned preparation
2The O composite joins in the LB liquid nutrient medium, the composite stoste of preparation 1000mg/L, and half-light is stored subsequent use down.
The stoste of above-mentioned preparation is carried out the bacteriostatic test to Aeromonas hydrophila and vibrio parahaemolytious;
Wherein Aeromonas hydrophila is the vibrionaceae Aeromonas, is the Gram-negative brevibacterium, and extreme single flagellum does not have brood cell and pod membrane, and normal two of the pathogen that has just separated from focus links to each other; Being distributed widely in natural various water body, is the primary pathogenic bacteria of multiple aquatic animal; Can the very strong exotoxin of toxigenicity, like hemolysin, histotoxin, necrotoxin, enterotoxin and protease etc., be the pathogen of bacteremic septicemia, can cause the generation of various freshwater fish septicemia.The model of the septicemia cause of disease can be described as: after Aeromonas hydrophila is invaded the fish body, in enteron aisle, rise in value earlier, get into liver, kidney and hetero-organization thereof through arterial circulation again, cause liver, kidney and other organs and dysemia, occur constitutional symptom then.This bacterium is a conditioned pathogen, and when the environment cataclysm, during water quality deterioration, regular meeting and other bacterium (like gentle aerogenesis monad, vibrios etc.) mixed infection aggravates disease.The general patient's condition of disease by Aeromonas hydrophila infects is more violent, is mostly pernicious infectious disease, and lethality is very high, and septicemia already causes heavy economic loss often for freshwater fish culturing.
Vibrio parahaemolytious is the halophagia Gram-negative bacteria, is distributed in bay, zone, coastline, salt lake and the marine product.Known vibrio parahaemolytious has O antigen and 59 kinds of K antigens in 12, can be divided into 5 types according to the situation of its fermenting carbohydrate.This bacterium all has stronger virulence to humans and animals, and its morbid substance mainly contains the pyrogenicity property hemolysin (TDH) of molecular weight 42000 and the similar hemolysin of TDH (TRH) of molecular weight 48000, has hemolytic activity, enterotoxin and lethal effect.Be cardinal symptom with Acute onset, stomachache, vomiting, diarrhoea and watery stool clinically.Main pathology is changed to jejunum and ileum has slight erosion, esogastritis, internal organ (liver, spleen, lung) extravasated blood etc.
Then measure the minimum inhibitory concentration (MIC) of composite with doubling dilution: 10 sterile test tube are labelled, and 10 groups of composite collosol concentration gradients of preparation are respectively the ultimate density of composite: 200mg/L under aseptic condition; 100mg/L, 50mg/L, 25mg/L; 12.5mg/L, 6.25mg/L, 3.13mg/L; 1.56mg/L, 0.78mg/L, 0 (control group).
Aeromonas hydrophila and vibrio parahaemolytious are cultivated respectively in the LB liquid nutrient medium, then above-mentioned two kinds of bacterium are pressed 1.0-0.1 * 10 respectively
6During 10 groups of composite colloidal sol test tubes that the inoculation bacterium of cfu/mL amount is inoculated into the different gradients of above-mentioned setting were respectively put, in about 37 ℃ of shaken cultivation 15h, not having the minimum composite concentration of bacterial growth with perusal was MIC.
The MIC of every kind of bacterium be higher than in the test tube of MIC concentration; Drawing the 0.1mL culture transfers respectively on the LB solid culture medium; Cultivate 24h for 37 ℃; Observation has or not bacterial plaque to form respectively then, and the composite concentration in the corresponding test tube that no bacterial plaque forms is the MBC (MBC) (referring to table 1 and table 2) of composite to this kind bacterial strain.
Table 1 is the MIC of two kinds of pathogens
Wherein, " +++" represent highly muddiness, " ++ " expression moderate is muddy, and "+" expression has bacteria growing, and "-" representes asepsis growth.
Table 2 is the MBC of two kinds of pathogens
Wherein, "+" expression has bacteria growing, "-" expression asepsis growth.
Visible by table 1: composite to the MIC of Aeromonas hydrophila and vibrio parahaemolytious all less than 500mg/L; Wherein to the MIC of Aeromonas hydrophila less than 50mg/L; MIC to vibrio parahaemolytious surpasses 100mg/L, thereby learns that composite is more obvious to the Aeromonas hydrophila inhibitory action.Visible by table 2; Composite to the MBCs (MBC) of two kinds of pathogens all greater than minimal inhibitory concentration (MIC); Composite shows when 50mg/L has bactericidal action to Aeromonas hydrophila, and vibrio parahaemolytious is just had the sterilization phenomenon when the 500mg/L.It is thus clear that composite is higher than vibrio parahaemolytious to the fungistatic effect of Aeromonas hydrophila.
Embodiment 2
Take by weighing 0.01g, 0.05g, 0.10g, 1.00g, the calcining shell/nanometer Cu of the above-mentioned preparation of 2.00g
2The O composite joins in the conical flask that fills LB liquid nutrient medium (volume is 10mL), and under aseptic condition, being mixed with mass concentration is 1000mg/L, 5000mg/L, and 10000mg/L, 100000mg/L, the colloidal sol of 200000mg/L is stored in the dark place.
Aeromonas hydrophila and vibrio parahaemolytious are cultivated respectively in the LB liquid nutrient medium, and (concentration of bacterium liquid is 1.0-0.1 * 10 with the bacteria suspension of LB liquid nutrient medium dilution to get 1mL respectively
6Cfu/mL) be inoculated in the sterile test tube that posts label, in bacterium liquid, add the composite colloidal sol of the variable concentrations of preparing more than the 0.01mL respectively, make that each in vitro final composite concentration is 10mg/L; 50mg/L; 100mg/L, 1000mg/L, 2000mg/L.Adopting ultraviolet light and sunshine respectively is light source irradiation, when using ultraviolet light as light source, with the 8W uviol lamp (Shanghai Ya Ming Lighting Co., Ltd) of a dominant wavelength 254nm as light source; When using sunshine, select the fine 10:00-15:00 time period to shine as light source.Dark reaction experiment and ultraviolet light illumination experiment are all carried out in superclean bench, and the distance between light source and the test tube is 10cm.After 60min is carried out in this experiment, sampling and observation experiment result, the composite that obtains variable concentrations respectively under unglazed, UV-irradiation, solar light irradiation condition to the killing effects of two kinds of bacterium.
Aeromonas hydrophila and vibrio parahaemolytious are cultivated respectively in the LB liquid nutrient medium, and (concentration of bacterium liquid is 1.0-0.1 * 10 with the bacteria suspension of LB liquid nutrient medium dilution to get 1mL respectively
6Cfu/mL) be inoculated in the sterile test tube that posts label, in bacterium liquid, adding the 0.01mL mass concentration is the composite colloidal sol of 10000mg/L, makes that in vitro final composite concentration is 100mg/L.Under unglazed, UV-irradiation, solar light irradiation, carry out sterilization experiment respectively, every separated 30min sampling once promptly respectively 30,60,90, is taken a sample during 120min.Obtaining about different time is the composite influence of sterilizing rate under unglazed, UV-irradiation, solar light irradiation condition respectively of 100mg/L to mass concentration.
Above-mentioned employing UV-irradiation and solar light irradiation are established the blank group respectively: do not add composite in (1) bacteria suspension, unglazed photograph (sterilization compares under light and the no optical condition in order to have); (2) bacteria suspension does not add composite; The identical time of illumination (for the composite photo-catalyst compares); Every group of parallel laboratory test 3 examples; The sterilizing rate of measuring composite with the method for plate culture count is to estimate its anti-microbial property, and the sterilizing rate computing formula is: clump count * 100% (referring to table 3, table 4, Fig. 1 and Fig. 2) of sterilizing rate=(clump count of the clump count-seminar of blank group)/blank group.
The variable concentrations composite is to the killing effect (time is 60min) of two kinds of bacterium under the no optical condition of table 3
The different time composite is to the killing effect (the composite mass concentration is 100mg/L) of two kinds of bacterium under the no optical condition of table 4
Table 3 is visible, and behind the composite that is lower than 2000mg/L when concentration and the two kinds of bacterium effect 60min, its survival rate can reach more than 80%, and this explains that composite is not obvious to the killing effect of two kinds of bacterium under the no optical condition.Table 4 is visible, and the killing rate of the composite of 100mg/L after to two kinds of bacterium effect 2h is very low when unglazed.
Visible by Fig. 1 simultaneously, to two kinds of kill pathogenic bacteria DeGrains, concentration is that the above bactericidal effect of 100mg/L then significantly improves to the composite of low concentration under ultraviolet light and sunshine.Visible by Fig. 2, under UV-irradiation, composite all has good killing effect to two kinds of aquatic pathogenic bacteriums.The composite colloidal sol of 100mg/L has the strong ability of killing to Aeromonas hydrophila and vibrio parahaemolytious, under ultraviolet light, behind the catalysis 2h killing rate of vibrio parahaemolytious is surpassed 98%, and the killing rate of Aeromonas hydrophila is reached 100%.In addition; When composite concentration is 100mg/L; No matter be almost to be killed entirely with bacterium after ultraviolet light or the sunlight catalytic sterilization 2h, this shows that composite only needs solar light irradiation in natural environment, need not extra use artificial light sources and just can have good bactericidal effect.
The described composite of the invention described above can be used as fish or the shrimp bactericide to Aeromonas hydrophila and vibrio parahaemolytious; Can composite be whitewashed the breed pool wall of fish, shrimps as coating, also can directly be ground into the powder spreading or process the aerosol jet crops.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1. calcine shell/nanometer Cu for one kind
2The application of O composite is characterized in that: calcining shell/nanometer Cu
2The O composite has bactericidal action.
2. the described calcining shell of claim 1/nanometer Cu
2The application of O composite is characterized in that: said calcining shell/nanometer Cu
2The O composite can be used as the bactericide that suppresses Aeromonas hydrophila or vibrio parahaemolytious.
3. by the described calcining shell of claim 1/nanometer Cu
2The application of O composite is characterized in that: said calcining shell/nanometer Cu
2The preparation of O composite is the conch meal that in the little reaction system of the Thermodynamically stable of being made up of NaCl and neopelex, adds CuCl and high-temperature activation; Continue to stir, utilize the flourishing hole of activation conch meal and basicity to make the cuprous salt hydrolysis and closely be carried on the conch meal surface; Through Separation of Solid and Liquid, ultrasonic washing, distilled water flushing, dehydrate after, synthetic shell/nanometer Cu
2The O composite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110200958.1A CN102349543B (en) | 2011-07-08 | 2011-07-08 | Application of calcined shell/nanometer Cu2O composite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110200958.1A CN102349543B (en) | 2011-07-08 | 2011-07-08 | Application of calcined shell/nanometer Cu2O composite |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102349543A true CN102349543A (en) | 2012-02-15 |
| CN102349543B CN102349543B (en) | 2014-01-08 |
Family
ID=45573270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110200958.1A Expired - Fee Related CN102349543B (en) | 2011-07-08 | 2011-07-08 | Application of calcined shell/nanometer Cu2O composite |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102349543B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886127A (en) * | 2014-03-05 | 2015-09-09 | 北京建元天地环保科技有限公司 | Manufacturing method of natural anti-bacterial and anti-virus agent |
| CN107474601A (en) * | 2017-09-18 | 2017-12-15 | 合肥品冠环保科技有限责任公司 | A kind of compound diatomite formulation for coating material of oyster shell whiting and preparation method thereof |
| CN113504369A (en) * | 2021-06-23 | 2021-10-15 | 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） | Individual correction formula for eliminating positive interference of serum nerve specific enolase detection caused by specimen hemolysis and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101151967A (en) * | 2006-09-28 | 2008-04-02 | 广东海洋大学 | Nano antimicrobial powder body with shell powder as carrier and method for preparing the same |
| CN101455436A (en) * | 2008-12-26 | 2009-06-17 | 江苏维斯康纳米银有限公司 | Beiyin detoxification degerming antistaling agent and manufacture method and use thereof |
| WO2010136792A2 (en) * | 2009-05-26 | 2010-12-02 | Intrinsiq Materials Limited | Antibacterial composition |
| CN101933525A (en) * | 2009-07-03 | 2011-01-05 | 中国科学院过程工程研究所 | Preparation method of nanometer Cu2O-CuO/Sr3BiO5.4 composite visible light sterilization catalyst |
| CN102107138A (en) * | 2009-12-25 | 2011-06-29 | 中国科学院海洋研究所 | Photocatalyst, preparation method and application thereof |
-
2011
- 2011-07-08 CN CN201110200958.1A patent/CN102349543B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101151967A (en) * | 2006-09-28 | 2008-04-02 | 广东海洋大学 | Nano antimicrobial powder body with shell powder as carrier and method for preparing the same |
| CN101455436A (en) * | 2008-12-26 | 2009-06-17 | 江苏维斯康纳米银有限公司 | Beiyin detoxification degerming antistaling agent and manufacture method and use thereof |
| WO2010136792A2 (en) * | 2009-05-26 | 2010-12-02 | Intrinsiq Materials Limited | Antibacterial composition |
| WO2010136792A3 (en) * | 2009-05-26 | 2011-06-16 | Intrinsiq Materials Limited | Antibacterial composition comprising metal oxide nanoparticles |
| CN101933525A (en) * | 2009-07-03 | 2011-01-05 | 中国科学院过程工程研究所 | Preparation method of nanometer Cu2O-CuO/Sr3BiO5.4 composite visible light sterilization catalyst |
| CN102107138A (en) * | 2009-12-25 | 2011-06-29 | 中国科学院海洋研究所 | Photocatalyst, preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《无机化学学报》 20091110 闫丽丽，等 "Cu2O纳米阵列的铜阳极氧化法制备及其光催化杀菌性能研究" 1960-1964 1-3 第25卷, 第11期 * |
| 闫丽丽，等: ""Cu2O纳米阵列的铜阳极氧化法制备及其光催化杀菌性能研究"", 《无机化学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886127A (en) * | 2014-03-05 | 2015-09-09 | 北京建元天地环保科技有限公司 | Manufacturing method of natural anti-bacterial and anti-virus agent |
| CN107474601A (en) * | 2017-09-18 | 2017-12-15 | 合肥品冠环保科技有限责任公司 | A kind of compound diatomite formulation for coating material of oyster shell whiting and preparation method thereof |
| CN113504369A (en) * | 2021-06-23 | 2021-10-15 | 中山大学肿瘤防治中心（中山大学附属肿瘤医院、中山大学肿瘤研究所） | Individual correction formula for eliminating positive interference of serum nerve specific enolase detection caused by specimen hemolysis and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102349543B (en) | 2014-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kale et al. | The diatoms: Big significance of tiny glass houses | |
| Hostins et al. | Managing input C/N ratio to reduce the risk of acute hepatopancreatic necrosis disease (AHPND) outbreaks in biofloc systems–a laboratory study | |
| CN107404862B (en) | Method for cultivating oysters on land | |
| AU2018307356B2 (en) | Efficient production of Pichia yeasts and their use for enhancing plant and animal health | |
| DK202070450A1 (en) | Probiotic bacteria for fish | |
| CN109618997A (en) | The ecological purification method of oyster culture | |
| US10500236B2 (en) | Bioactive animal feed | |
| KR101746667B1 (en) | Chimeric peptide having antialgae activity and composition for control of red tide comprising the same | |
| CN102349543B (en) | Application of calcined shell/nanometer Cu2O composite | |
| CN1219430C (en) | Youngling of large sea horse and artificial cultivating method of commodity sea horse and its equipment | |
| JP2007267656A (en) | Method for breeding young fry | |
| CN109496969A (en) | A kind of preventing control method of livestock and poultry cultivation disease | |
| CN1034842A (en) | A kind of method of producing the algae of the biological effect that tool improved | |
| CN102919183B (en) | Method for effectively inhibiting vibrio breeding in shrimp culture | |
| KR101800810B1 (en) | Method for prevention and treatment for scuticociliatosis in fishes using blue light emitting diode(LED) | |
| Vibhute et al. | Applications of Nanoparticles in Aquaculture | |
| CN106614650B (en) | Application of rotenone in inhibition of egg laying of rotifers | |
| Guasto et al. | Arthrospira platensis | |
| JP6845251B2 (en) | How to sterilize marine animals | |
| Osinga et al. | Feeding corals in captivity: uptake of four Artemia-based feeds by Galaxea fascicularis | |
| CN104257688A (en) | Combined disinfection method for industrial aquaculture of flounders | |
| CN104126529B (en) | A kind of method controlling the total bacteria that Copepods is carried | |
| KR20230095509A (en) | The functional salt using biofloc breeding water and manufacturing method there of | |
| CN115152674A (en) | Method for breeding oysters capable of being eaten raw | |
| EA017600B1 (en) | Thermophilic strain of blue-green algae spirulina platensis baku for production of biomass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140108 Termination date: 20200708 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |