CN102334587A - Processing method for efficiently removing deoxynivalenol (DON) from wheat grains - Google Patents
Processing method for efficiently removing deoxynivalenol (DON) from wheat grains Download PDFInfo
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Abstract
The invention discloses a processing method for efficiently removing deoxynivalenol (DON) from wheat grains. The method for removing the DON from the DON-polluted wheat grains comprises the following steps of: making the DON-polluted wheat grains into flour, and extracting gluten from the flour to obtain the gluten without the DON; and then removing the DON from the DON-polluted wheat grains. In the detoxicated gluten obtained by the processing method, no DON toxin is detected so as to fully meet the national standard requirements. The processing method has the beneficial effects that the DON can be removed from the wheat grains by simple processing and extraction technologies without damage on the quality of the original product as well as secondary pollution; and the produced detoxicated gluten can be taken as a semi-finished product to be reprocessed.
Description
Technical field
The present invention relates to the processing method of deoxynivalenol in a kind of efficient removal wheat seed.
Background technology
Deoxynivalenol (DON) is an a kind of trichothecene family toxin, is mainly produced by Fusarium graminearum and Fusarlum roseum.These fungies grow in wheat, produce a large amount of DON toxin and pollute wheat, and cause common disease---the head blight of wheat.Trichothecene family toxin is decided to be the most dangerous naturally-occurring food contaminant; And wherein DON pollution grain (wheat, corn, barley etc.) is the most general; Its stability is strong, has very strong cytotoxicity and genetoxic, and the people takes in by after the cereal of its pollution with other animals; Can cause apocleisis, vomiting, diarrhoea, fever, astasia and acute poisoning symptom such as slow in reacting, serious harm people and animals' safety.Especially in southern wheat main product such as China's Yangze river and Huai river area, weathers such as the rainy and flood of the continuous high temperature of wheat after warm rainy, the summer has set in of blooming stage to ripening period are easier to the generation of head blight, cause a large amount of wheats to pollute the DON toxin.
For this reason; The Ministry of Public Health issued one in 2010 about being strictly on guard against that the edible wheat that goes mouldy causes the bulletin of food poisoning; Wherein require grain operation, processing enterprise and relevant food production and operation unit should strengthen the management to the grain processing process, the wheat processing of need not going mouldy is made and sold food.Yet; If research and develop a kind of technology that can efficiently remove DON toxin in the pollution wheat; Its DON content of toxins is reduced in its threshold limit values level (among the GB 2761-2005 in regulation wheat and the goods thereof DON content be 1 μ g/g), dwindles the degree that it gets into food chain, reduce murder by poisoning human body; And can the wheat that pollute the DON toxin be utilized again, reduced China greatly and suffered from the diminish waste of wheat of head blight.
At present, remove the method for polluting DON content of toxins in the wheat, mainly can be divided into following five types:
1) physical process detoxification: common physical method has rinsing grinding, ionizing radiation, inorganic absorption, and wherein these methods have been used to reduce the adverse effect of fusarium toxin to animal husbandry, but this method should not be used for food processing.
2) chemical process detoxification: processing (like ammonia, sodium hydrogensulfite, clorox), heat treatment, ozone oxidation that comprises chemical reagent etc. tentatively has been used to purify the cereal that contains DON.The detoxification efficiency of these chemical processes is good, but the safety problem that thereupon produces after the wherethrough reason is troubling equally.Simultaneously, high temperature can destroy other nutritional labelings in the food, so not as the main means of detoxification.
3) biological process detoxification: in recent years, adopt the mycotoxin in the method for microorganism control food chain to be paid close attention to by scientific circles, comprise the thalline absorption of microorganism and two main paties of degrading.Wherein find the DON of dark red type of yeast, red type of yeast of glue, the strange yeast of U.S. utmost point plum, geotrichum fermentans yeast, Kluyveromyces marxianus discovery ability adsorptive liquid; The microbial degradation of DON is research emphasis in recent years, and can the degrade DON of part of discovery and microorganism in bovine rumen and the soil, and mechanism of degradation is among further research.
4) molecular biology and the detoxification of plant breeding process: mainly be through seeking, identify wheat plant opposing DON toxin quantitative trait locus (QTLs).And be found to gene segment, and through discovering that this part has detoxication to DON with UDP-glucosyltransferase coding.
5) other process detoxifications: crop rotation and the method for suitably ploughing help to reduce or avoid plant to suffer the pollution of fusarium toxin.
Summary of the invention
An object of the present invention is to provide a kind of removal by the method for deoxynivalenol in the wheat seed of deoxynivalenol pollution.
Removal provided by the present invention is by the method for deoxynivalenol in the wheat seed of deoxynivalenol pollution; May further comprise the steps: will be processed flour by the wheat seed that deoxynivalenol pollutes; From said flour, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol; Thereby remove the deoxynivalenol in the wheat seed that is polluted by deoxynivalenol.
Another object of the present invention provides a kind of method of using the wheat seed preparation of being polluted by deoxynivalenol not contain the gluten of deoxynivalenol.
The method of using the wheat seed preparation of being polluted not contain the gluten of deoxynivalenol provided by the present invention by deoxynivalenol; May further comprise the steps: will be processed flour by the wheat seed that deoxynivalenol pollutes; From said flour, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol.
In the said method, the wheat seed present general-purpose industrial powder process of process earlier or two kinds of approach of experiment powder process of being polluted by deoxynivalenol obtain long patent flour, and long patent flour obtains gluten through the operation that gluten extracts again.
Wherein, industrial powder process and experiment powder process are all taked be vertical current to, layering mill, the principle of ground gently and separated carefully.Industry powder process generally is made up of break system, reduction system, sizing system and powder-cleaning system, adopts brabender experiment mill, cloth to rein in experiment mills such as experiment is ground, homemade Wuxi tin grain experiment mill and test powder process, generally only is made up of break system and reduction system.
The said method that obtains gluten of from said flour, extracting may further comprise the steps: in said flour, add entry or sodium-chloride water solution, process dough; Starch in water or the said dough of sodium-chloride water solution flush away promptly obtains gluten.
The measuring that in said flour, adds entry or sodium-chloride water solution is to add the sodium-chloride water solution that adds 46ml～52ml or 46ml or 49ml or 52ml in water or the said flour of every 100g of 46ml～52ml or 46ml or 49ml or 52ml in the said flour of every 100g.
The concentration of said sodium-chloride water solution is 0g/L～20g/L or 1g/L or 10g/L or 20g/L, but is not 0g/L.
The standard of the starch in the said dough of said flush away is following 1) or 2) shown in:
1) water of from the said gluten that obtains, extruding is water white transparency;
2) in the water of from the said gluten that obtains, extruding, add KI/iodine solution after, the color of said water does not change.
Another purpose of the present invention provides a kind of removal by the method for deoxynivalenol in the flour of deoxynivalenol pollution.
Removal provided by the present invention is by the method for deoxynivalenol in the flour of deoxynivalenol pollution; May further comprise the steps: from the flour that is polluted by deoxynivalenol, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol.
Another purpose of the present invention provides a kind of method for preparing the gluten that does not contain deoxynivalenol in the flour that is polluted by deoxynivalenol of using.
The method for preparing the gluten that does not contain deoxynivalenol in the flour that is polluted by deoxynivalenol of using provided by the present invention may further comprise the steps: from the flour that is polluted by deoxynivalenol, extract and obtain gluten; The gluten that is not promptly contained deoxynivalenol.
The said method that obtains gluten of from said flour, extracting may further comprise the steps: in said flour, add entry or sodium-chloride water solution, process dough; Starch in water or the said dough of sodium-chloride water solution flush away promptly obtains gluten.
The measuring that in said flour, adds entry or sodium-chloride water solution is to add the sodium-chloride water solution that adds 46ml～52ml or 46ml or 49ml or 52ml in water or the said flour of every 100g of 46ml～52ml or 46ml or 49ml or 52ml in the said flour of every 100g.
The concentration of said sodium-chloride water solution is 0g/L～20g/L or 1g/L or 10g/L or 20g/L, but is not 0g/L.
The standard of the starch in the said dough of said flush away is following 1) or 2) shown in:
1) water of from the said gluten that obtains, extruding is water white transparency;
2) in the water of from the said gluten that obtains, extruding, add KI/iodine solution after, the color of said water does not change.
The invention provides a kind of efficient removal by the processing method of deoxynivalenol (DON) in the wheat seed of deoxynivalenol pollution.Utilize in the detoxification product gluten that this method obtains, the DON toxin fails to detect, and complies with the national standard requirements fully.This method can reach the purpose of DON in the great removal wheat seed through simple processing and extraction process; Do not destroy simultaneously the quality of original product; Do not produce secondary pollution; The detoxification gluten of producing can be used as semi-finished product and reprocesses, and obtains elementary gluten converted products such as fried gluten puff, water gluten, steamed gluten, cool skin, gluten intestines, gluten skin, gluten silk, or is added with the whole wheat of gluten so that make bread or biscuit, macaroni and the similar nutraceutical of some type; Also, be used to make the article of some glue and production sulfuric acid glutelin or phosphoric acid glutelin, hydrolyzed vegetable protein and sodium glutamate and so on as the adhesive of some meat product.
Description of drawings
Fig. 1 is for removing the process route view of deoxynivalenol in the wheat seed.
Fig. 2 utilizes high performance liquid chromatography to detect the calibration curve of the concentration (μ g/ml) of the deoxynivalenol (DON) that obtains to peak height.
Fig. 3 is for detecting the liquid chromatogram of DON content of toxins in the wheat seed.
The specific embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
The wheat seed that the present invention pollutes deoxynivalenol (DON) toxin with summer in 2010 from southern wheat belts such as China's Yangze river and Huai river is an example, and the process route of the method for deoxynivalenol (DON) toxin in the removal wheat is as shown in Figure 1.Its concrete steps can be: take by weighing the wheat 1000g that pollutes the DON toxin; Test the powder process standard with reference to wheat: first: equipment, sample preparation and wheat wetting (NY/T 1094.1-2006) and the 5th part: Brabender Quadrumat Jr (Quadruplex) tests mill method (NY/T 1094.5-2006); Adopt brabender experiment mill Quadrumat (Quadruplex), obtain flour.Or from the market in southern wheat areas such as Yangze river and Huai river, buy the flour that local flour mill draws through industrial powder process with the wheat seed that polluted deoxynivalenol (DON) toxin in 2010.From said flour, get 100g or 1000g flour, be example, again to wherein adding the certain density sodium chloride or the aqueous solution, form dough after, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the water that extrude this moment was colourless.Draining press dry (or to be used centrifuge speed 6000r/min, obtains wet gluten after 5min), further obtain other various gluten goods again.
Deoxynivalenol (DON) toxin in embodiment 1, the removal wheat seed
The method of extracting and detect DON content of toxins in the wheat seed is following:
A) extraction of DON
Driedly pulverized with pulverizer getting, accurately take by weighing 5g, place the 100mL triangular flask by the wheat seed sample of DON endotoxin contamination (collection) from wheat belt, China Yangze river and Huai river south; Add 35mL acetonitrile/water solution (84: 16, V/V), with the disperser 3min that vibrates; After mixing, pour in the volumetric flask, added acetonitrile/water solution (84: 16; V/V) be settled to 50mL,, obtain supernatant the whole sample liquid of the folding filtration of qualitative filter paper.
Draw supernatant 4mL, behind the Bond Elut Mycotoxin decontaminating column, accurately draw scavenging solution 3mL excessively, be transferred in the scale test tube, under 50 ℃ in nitrogen purging appearance, slowly be blown into nitrogen, be evaporated to dried.
After the drying, add 1.5mL flowing phase, behind the concussion 1.5min mixing, change the 2mL centrifuge tube over to, 10,000rpm high speed centrifugation 6min, centrifuged supernatant is put in the 2mL micro-sampling bottle, and is to be measured.B) high effective liquid chromatography for measuring of DON:
Get 2mL micro-sampling bottle, be put on the LC system sample introduction dish, carry out liquid-phase chromatographic analysis.
Wherein, the liquid chromatographic detection condition is following:
Chromatographic column: 3.9mm * 150mm, 5 μ m.
Flowing phase: acetonitrile/methanol/water (5/5/90, V/V/V)
Flow velocity: 1.0mL/min
Detector: UV-detector, detect wavelength: 220nm.
Column temperature: 40 ℃.
Sample size: 10 μ L.
By above-mentioned chromatographic condition, sample liquid is carried out liquid-phase chromatographic analysis, with the external standard standard measure, chem workstation carries out data.The retention time of wheat samples liquid to be measured is that 4.690 peak height is 1793.The liquid phase collection of illustrative plates is as shown in Figure 3.
C) making of DON calibration curve
The DON standard liquid: with flowing phase solution (acetonitrile/methanol/water, 5/5/90, V/V/V) be mixed with 10 μ g/mLDON standard liquids; Again with flowing phase solution preparation calibration curve working concentration 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, 6 μ g/mL, 8 μ g/mL and 10 μ g/mL; Making abscissa with concentration, is that the peak height at 0.05min place before and after the 4.690min is made ordinate with the retention time, carries out linear regression according to the relation of concentration and peak height; And the drawing standard curve, as shown in Figure 2.The calibration curve equation that obtains according to calibration curve is: y=975.73x-4.10 (y is a peak height, and x is a DON content).The content that uses the calibration curve equation to calculate DON in the wheat samples liquid to be measured is 1.842 μ g/mL.The DON content of toxins that conversion obtains in this wheat seed is 9.21 μ g/g.Reduction formula is following:
Take by weighing by the wheat of DON endotoxin contamination (available from Anhui province Yangze river and Huai river wheat main producing region) 1000g,, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 476.15g flour according to NY/T1094.1-2006 and NY/T 1094.5-2006.Use with said extracted with detect the method that the DON content of toxins is identical in the wheat and extract and detect DON content of toxins in the flour, the result is 7.33 μ g/g for DON content of toxins in the above-mentioned flour that obtains.Get 100g flour, again to the sodium-chloride water solution that wherein adds 46ml 20g/L, process dough after, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry matter content) in the gluten.It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 2, the removal wheat seed
Take by weighing with embodiment 1 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 9.21 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 466.28g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 7.33 μ g/g in the flour.Get 100g flour, again to the sodium-chloride water solution that wherein adds 52ml 1g/L, process dough after, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: after in the water of from gluten, extruding, adding KI/iodine solution, the color of water did not change.Gluten is obtained wet gluten with centrifuge behind the centrifugal 5min of rotating speed 6000r/min, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 3, the removal wheat seed
Take by weighing with embodiment 1 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 9.21 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 477.76g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 7.33 μ g/g in the flour.Get 100g flour, again after wherein adding the 46ml drinking water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading; Till starch in gluten is cleaned; The criterion that starch in the gluten is cleaned is: the water of from gluten, extruding is colourless; Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 4, the removal wheat seed
Take by weighing with embodiment 1 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 9.21 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 482.43g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 7.33 μ g/g in the flour.Get 100g flour, again after wherein adding the 52ml running water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 5, the removal wheat seed
Take by weighing by the wheat seed of DON endotoxin contamination (available from Yangze river and Huai river wheat main producing region, Jiangsu Province) 1000g, being used for the identical method of embodiment 1, to detect the DON content of toxins that obtains in this wheat seed be 4.23 μ g/g; According to NY/T1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 513.83g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 2.91 μ g/g in the flour.Get 100g flour, again to the sodium chloride solution that wherein adds 49ml 20g/L, process dough after, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned was: after in the water of from gluten, extruding, adding iodine solution, the color of water does not change.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 6, the removal wheat seed
Take by weighing with embodiment 5 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 4.23 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 527.22g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 2.91 μ g/g in the flour.Get 100g flour, again to the sodium chloride solution that wherein adds 46ml 10g/L, process dough after, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 7, the removal wheat seed
Take by weighing with embodiment 5 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 4.23 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 516.51g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 2.91 μ g/g in the flour.Get 100g flour, again after wherein adding the 49ml drinking water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned was: after in the water of from gluten, extruding, adding iodine solution, the color of water does not change.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 8, the removal wheat seed
Take by weighing with embodiment 5 in identical by the wheat seed 1000g of DON endotoxin contamination, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this wheat seed be 4.23 μ g/g; According to NY/T 1094.1-2006 and NY/T 1094.5-2006, adopt brabender experiment mill Quadrumat (Quadruplex), obtain 521.90g flour.Use with embodiment 1 in identical method detection obtain that the DON content of toxins is 2.91 μ g/g in the flour.Get 100g flour, again after wherein adding the 46ml running water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 9, the removal wheat
Take by weighing from the market in southern wheat such as Yangze river and Huai river area and buy local flour mill with the flour 5000g that the wheat seed that polluted deoxynivalenol (DON) toxin in 2010 draws through industrial powder process, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this flour be 0.87 μ g/g; Get wherein 1000g flour, again after wherein adding the 520ml drinking water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Deoxynivalenol (DON) toxin in embodiment 10, the removal wheat
Take by weighing from the market in southern wheat such as Yangze river and Huai river area and buy local flour mill with the flour 5000g that the wheat seed that polluted deoxynivalenol (DON) toxin in 2010 draws through industrial powder process, being used for embodiment 1 identical method, to detect the DON content of toxins that obtains in this flour be 0.50 μ g/g; Get wherein 1000g flour, again after wherein adding the 460ml running water, processing dough, with suitable flow washing dough, the rubbing dough that does not stop simultaneously.And the gluten ball that will form continues with running water flushing, kneading, and till the starch in gluten was cleaned, the criterion that the starch in the gluten is cleaned is: the water of from gluten, extruding was colourless.Obtain wet gluten after draining press dry, wet gluten is dried to constant weight for 50 ℃, extract then and detect DON content of toxins (dry).It is identical with the method that said extracted detects DON content of toxins in the wheat to extract the method that detects DON content of toxins in the gluten.This experiment is established three times and is repeated and average.
Gluten sample liquid to be measured does not go out the peak at retention time 4.690min place, promptly in gluten, does not detect the DON toxin.
Claims (10)
1. a removal is by the method for deoxynivalenol in the wheat seed of deoxynivalenol pollution; May further comprise the steps: will be processed flour by the wheat seed that deoxynivalenol pollutes; From said flour, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol; Thereby remove the deoxynivalenol in the wheat seed that is polluted by deoxynivalenol.
2. method that does not contain the gluten of deoxynivalenol with the wheat seed preparation of being polluted by deoxynivalenol; May further comprise the steps: will be processed flour by the wheat seed that deoxynivalenol pollutes; From said flour, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol.
3. method according to claim 1 and 2 is characterized in that:
The said method that obtains gluten of from said flour, extracting may further comprise the steps: in said flour, add entry or sodium-chloride water solution, process dough; Starch in water or the said dough of sodium-chloride water solution flush away promptly obtains gluten.
4. according to the method described in the claim 3, it is characterized in that:
The said amount that in said flour, adds entry or sodium-chloride water solution is to add the sodium-chloride water solution that adds 46ml～52ml in water or the said flour of every 100g of 46ml～52ml in the said flour of every 100g.
5. according to the method described in the claim 3, it is characterized in that: the concentration of said sodium-chloride water solution is 0g/L～20g/L, but is not 0g/L.
6. a removal is by the method for deoxynivalenol in the flour of deoxynivalenol pollution; May further comprise the steps: from the flour that is polluted by deoxynivalenol, extract and obtain gluten, promptly do not contained the gluten of deoxynivalenol.
7. one kind does not contain the method for the gluten of deoxynivalenol with the flour preparation of being polluted by deoxynivalenol, may further comprise the steps: from the flour that is polluted by deoxynivalenol, extract and obtain gluten; The gluten that is not promptly contained deoxynivalenol.
8. according to claim 6 or 7 described methods, it is characterized in that:
The said method that obtains gluten of from said flour, extracting may further comprise the steps: in said flour, add entry or sodium-chloride water solution, process dough; Starch in water or the said dough of sodium-chloride water solution flush away promptly obtains gluten.
9. the method described in according to Claim 8 is characterized in that:
The said amount that in said flour, adds entry or sodium-chloride water solution is to add the sodium-chloride water solution that adds 46ml～52ml in water or the said flour of every 100g of 46ml～52ml in the said flour of every 100g.
10. the method described in according to Claim 8, it is characterized in that: the concentration of said sodium-chloride water solution is 0g/L～20g/L, but is not 0g/L.
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| CN103222536A (en) * | 2013-05-06 | 2013-07-31 | 国家粮食局科学研究院 | Industrial method for removing deoxynivalenol in wheat grains |
| CN104366162A (en) * | 2014-11-17 | 2015-02-25 | 江南大学 | Method for quickly reducing vomitoxin in wheat |
| CN104705538A (en) * | 2013-12-11 | 2015-06-17 | 中国农业科学院农产品加工研究所 | Boiling method to remove deoxynivalenol (DON) in flour products |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222536A (en) * | 2013-05-06 | 2013-07-31 | 国家粮食局科学研究院 | Industrial method for removing deoxynivalenol in wheat grains |
| CN104705538A (en) * | 2013-12-11 | 2015-06-17 | 中国农业科学院农产品加工研究所 | Boiling method to remove deoxynivalenol (DON) in flour products |
| CN104705538B (en) * | 2013-12-11 | 2017-11-03 | 中国农业科学院农产品加工研究所 | The method for cooking of deoxynivalenol in a kind of removal Flour product |
| CN105166600A (en) * | 2014-05-27 | 2015-12-23 | 中国农业科学院农产品加工研究所 | Method for lowering deoxynivalenol in fine dried noodles |
| CN105166600B (en) * | 2014-05-27 | 2018-12-14 | 中国农业科学院农产品加工研究所 | A kind of method of deoxynivalenol in reduction vermicelli |
| CN104366162A (en) * | 2014-11-17 | 2015-02-25 | 江南大学 | Method for quickly reducing vomitoxin in wheat |
| CN107589205A (en) * | 2017-09-30 | 2018-01-16 | 中南粮油食品科学研究院有限公司 | A kind of method that deoxynivalenol in wheat is detected based on high performance liquid chromatography |
| CN108554481A (en) * | 2018-04-11 | 2018-09-21 | 涡阳县丰华面业有限公司 | A kind of wheat wetting method of mycotoxin levels in reduction wheat |
| CN108872143A (en) * | 2018-05-22 | 2018-11-23 | 南京农业大学 | A kind of wheat infection head blight level detection method based near infrared spectrum |
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