CN102329804B - Dehydrated response element binding protein in arabidopsis thaliana and coding gene and applications thereof - Google Patents
Dehydrated response element binding protein in arabidopsis thaliana and coding gene and applications thereof Download PDFInfo
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Abstract
The invention relates to cloning, recombination and applications of dehydrated response element binding protein 2A gene (AtDREB2A) in arabidopsis thaliana. The gene has nucleotide sequence shown in SEQ ID No.1. The total RNA is extracted from Arabidopsis thaliana leaves, reverse transcription PCR is carried out, the AtDREB2A gene is obtained and is connected onto a PBS-TII vector, and an intermediate vector PBS-TII-AtDREB2A is obtained. The PBS-TII-AtDREB2A is used as a template, the dehydrated response element binding protein 2A combined activated gene (AtDREB2A-CA) in the arabidopsis thaliana is obtained by using a method for folding and extending PCR (SOE-PCR) to carry out PCR amplification, the amplified product is connected to a plant expression vector, and a plant expression vector pH7m24GW, 3-Prd29A-AtDREB2A-CA is obtained. The vector is imported into agrobacterium tumefaciens C58 cells by an electric shock method, the cells are used for dip-dyeing the tobacco, then the tobacco into which the AtDREB2A-CA is transferred is obtained, and by test, the condition that the salt resistance of the transgenic tobacco is greatly improved is discovered.
Description
Technical field
The invention belongs to biology field; Be particularly related to a kind of Arabidopis thaliana (Arabidopsis thaliana) dehydration response original paper conjugated protein and encoding sox and application, be specifically related to clone, reorganization and the application of the conjugated protein 2A gene of dehydration response original paper (AtDREB2A) in the Arabidopis thaliana.
Background technology
Plant always directly lives in the physical environment complicated and changeable.Adverse circumstances such as arid, saline and alkaline, cold and high temperature can make plant receive the physiology injury, when serious even cause plant dead.In order to adapt to these adverse circumstances, plant develops in very long evolutionary process and the resistance mechanism to these abiotic stress, for example: the change of stomatal closure, cytolemma and kytoplasm composition etc.Plant can pass through signal transduction path under the effect of abiotic stress, make some expression of gene regulated and control by adverse circumstance, thereby replys (Shinozaki and Yamaguchi-Shinozaki, 1996) to coercing to make.Transcription factor is one type can be incorporated into specific DNA district specifically, and having transcribed of its downstream gene activated or inhibiting protein.Transcription factor in the plant plays the regulation and control environment stress and replys, the effect of related gene expressions such as cause of disease reaction and growth.CBF/DREB wherein (C-repeat-binding-factor/dehydration-responsive-element-b inding) transcription factor is the important component that the plant abiotic stress is replied; Behind the abduction delivering; Can activate the expression of many downstream adversity gene, thereby strengthen the resistance of plant.
Since 1997; The method of human yeast monobasics such as Stocking hybridization has been separated (Stockinger et al.1997) since the conjugated protein CBF1 of DRE/CRT from Arabidopis thaliana; Many DREB genes involveds (DREB-like) have been arrived; These gene products can combine the DRE/CRT element of promoter region, starting the expression of the abiotic resistant gene of a sequence, and give abilities such as plant salt tolerance, arid, cold, high temperature.(Gilmour?et?al.1998；Liu?et?al.1998；Shinwari?et?al.1998)。
It is generally acknowledged that different with plant disease-resistant mechanism, plant receives controlled by multiple genes to the resistance of adverse circumstances such as arid and high salt, therefore, utilize genetic engineering technique to import the individual feature gene and be difficult to improve significantly the resistance of plant.And the upstream gene in the signals-modulating approach; Cis-acting elements specificity that then can gene promoter region like transcription factor combines; In inducing the degeneration-resistant relevant functional gene expression in downstream, play a key effect, therefore utilize transcription factor enhancement of plant resistance to become the important channel of improvement stress resistance of plant.In salt resistant gene engineering field, DREB-directly receives much concern, and investigators import in the Plant Genome through transgenic means DREB, have obtained many resistance enhanced transformed varieties.
The DREB2A gene is the member of dreb gene family; Its full cDNA sequence links to each other with 35S promoter and changes Arabidopis thaliana over to; Discovery DREB2A under non-stress conditions can express; But downstream gene expression is faint, and the plant resistance is not improved significantly, and infers that DREB2A need pass through to transcribe post-treatment such as phosphorylation etc.Bioinformatic analysis shows that the proteic 136-165 amino acids of DREB2A residue is rich in the ubiquitin site, and the existence in these sites makes this albumen get into degradation pathway, thereby can not steady in a long-termly exist, the performance function.Perhaps, this is exist in the plant materials machine-processed to this proteic negative regulation.At home, all vast equality people import wheat simultaneously with Arabidopis thaliana DREB2A and bar gene, have measured the cotransformation frequency, but the salt resistance of transgenic wheat are not made assessment.We delete 136-165 amino acids among the DREB2A through the method for folding extension PCR, obtain the DREB2A-CA of constitutive activation.Through agriculture bacillus mediated transgenic method, its function of checking in transgene tobacco.Find that this gene can improve the anti-salt of plant, drought resisting, high-temperature resistance effectively.
Summary of the invention
The object of the present invention is to provide dehydration response original paper conjugated protein 2A constitutive activation (constitutive activation/CA) gene (AtDREB2A-CA) in a kind of Arabidopis thaliana source.
Second purpose of the present invention provides this recombination encoded protein matter.
A further object of the present invention also is to provide recombinant vectors and the host cell that contains this recombination.
Another object of the present invention is to provide the purposes of this gene.
The invention provides a kind of Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA), the nucleotide sequence shown in SEQ ID NO.1 in the sequence table constitutes.
The invention provides a kind of above-mentioned Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) encoded protein matter, the protein of the aminoacid sequence shown in SEQ ID NO.2 in the sequence table.
The invention provides a kind of above-mentioned Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) recombinant cloning vector pBS-T-AtDREB2A-CA.
Contain the recombinant vectors of above-mentioned Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA), these recombinant vectorss comprise plasmid and plant expression vector.
Described recombinant plant expression vector pH7m24GW, 3-Prd29A-DREB2A-CA.
Contain the host cell of the complete coding reading frame sequence of above-mentioned Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA), as the host cell that contains above-mentioned recombinant vectors also belongs to protection scope of the present invention.
Described host cell is selected from Bacillus coli cells, agrobatcerium cell or tobacco cell.
The invention provides the genetic engineering bacterium of a kind of AtDREB2A-CA of containing.
The application of above-mentioned Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) comprises the application of albumen in plant of this genes encoding; Use described recombinant vectors, like plant expression vector maize transformation cell; Perhaps, obtain genetically modified regeneration plant with said Agrobacterium and the co-culture of cells such as corn, soybean, paddy rice, peanut, Sunflower Receptacle, yam, cotton, millet, barley and flowers and vegetables that contain this gene; Perhaps obtain above-mentioned species transfer-gen plant with described AtDREB2A-CA genetic transformation.
Technical scheme of the present invention is concrete to be summarized as follows:
A kind of Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA); Nucleotide sequence shown in SEQ ID NO.1 in the sequence table comprises that also the nucleotide sequence shown in the SEQ ID No.1 add, replace, insert or delete 70% above homologous sequence or its allelotrope and the deutero-nucleotide sequence thereof of one or more Nucleotide.
A kind of Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) recombinant vectors pH7m24GW, Agrobacterium C58 of 3-Prd29A-DREB2A-CA of containing.
A kind of Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) encoded protein, aminoacid sequence shown in SEQ ID NO.2 in the sequence table.
A kind of Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) encoded protein is tobacco, the application in corn and the soybean.
Described Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) also is applied to prepare plant such as genetically engineered soybean, paddy rice, peanut, Sunflower Receptacle, yam, cotton, millet, barley and flowers and vegetables.
Cloning process of the present invention is made up of following step:
From Arabidopsis leaf, extract total RNA; Native sequences according to the conjugated protein 2A of Arabidopis thaliana dehydration response original paper (AtDREB2A) among the GenBank; Design is by the upstream primer P1 shown in the SEQ ID NO.3; With by the downstream primer P2 shown in the SEQ ID NO.4, the clone obtains the AtDREB2A gene, and this gene is connected the PBS-TII carrier.Obtaining intermediate carrier PBS-TII-AtDREB2A utilizes the amplification of folding extension PCR (SOE-PCR) method to obtain Arabidopis thaliana dehydration response original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) then.
The present invention makes up the plant expression vector pH7m24GW that contains the conjugated protein 2A constitutive activation of Arabidopis thaliana dehydration response original paper gene, and 3-Prd29A-DREB2A-CA is made up of following step:
Design is template by the upstream primer F1 shown in the SEQ ID NO.5 with by the downstream primer R1 shown in the SEQ ID NO.6 with PBS-TII-AtDREB2A, carries out pcr amplification, obtains first PCR fragment.Use the upstream primer F2 shown in the SEQ ID NO.7 again and, be template, carry out the pcr amplification second time, obtain second PCR fragment with PBS-TII-AtDREB2A by the downstream primer R2 shown in the SEQ ID NO.8.To appeal two fragments then is SOE-PCR and obtains a fusion dna fragment; Be AtDREB2A-CA, with this pcr amplification product after EcoRI and EcoRV enzyme are cut, with plant expression vector pH7m24GW; 3-Prd29A cuts through EcoRI and EcoRV enzyme; The two carries out ligation, obtains plant expression vector pH7m24GW, 3-Prd29A-DREB2A-CA.
The invention provides the conjugated protein 2A constitutive activation of a kind of Arabidopis thaliana dehydration response original paper gene-based because of and comprise recombinant vectors and the host cell and the application of this gene.Be connected to then on the plant expression vector, utilize Agrobacterium infestation method transformation of tobacco, the transfer-gen plant of acquisition has carried out the salt resistance analysis, and the result shows that the transgene tobacco saline-alkaline tolerance is improved.
Description of drawings
Fig. 1 is with the PCR electrophorogram of AtDREB2A primer to Arabidopis thaliana cDNA.
Fig. 2 AtDREB2A connects the PCR authentication result of PBS-TII carrier.
Fig. 3 PBS-TII-AtDREB2A carrier synoptic diagram.
Fig. 4 obtains the electrophoresis checking result of AtDREB2A-CA with SOE-PCR.
Fig. 5 plant expression vector pH7m24GW, 3-Prd29A-DREB2A-CA cuts checking with the HindiIII enzyme.
Fig. 6 plant expression vector pH7m24GW, the 3-Prd29A-DREB2A-CA synoptic diagram.
Fig. 7 tobacco transgenic process.
Fig. 8 transgene tobacco genome PCR.
The anti-salt property test of Fig. 9 transgene tobacco.
Embodiment
The clone of the conjugated protein 2A constitutive activation of Arabidopis thaliana dehydration response original paper Gene A tDREB2A gene and the structure of intermediate carrier pBS-T-AtDREB2A
With Arabidopis thaliana RNA is template, uses Reverse Transcription System (Promega company) test kit to carry out reverse transcription, synthetic cDNA first chain.
1. total RNA of 1 μ g (2 μ l) is placed the centrifuge tube of 1.5ml, 70 ℃ the heating 10min, rapidly in cooled on ice, of short duration centrifugal after, place on ice;
2. reverse transcription-reaction system following:
3. behind the mixing, 42 ℃ of temperature are bathed 50min;
4.95 ℃ heating 5min places 5min for 4 ℃, makes AMV reversed transcriptive enzyme inactivation, no longer combines with the cDNA chain;
5. getting 1 μ LcDNA reaction solution is template, carries out the PCR reaction, uses the AtDREB2A gene specific primer, SEQ ID NO.3 and SEQ ID NO.4:
F5′-GGGAAGGAGATGGCAGTTT-3′19nt
R5′-GTTGTGGGATTAAGGCAAATA-3′21nt
The PCR reaction system is following:
The pcr amplification condition is:
Get 5 μ L PCR products and carry out detected through gel electrophoresis.Electrophoresis result is seen shown in Figure 1.
According to the method described above the PCR fragment of dreb gene is connected the pBS-TII carrier then,
AtDREB2A gene and pBS-TII carrier are linked,
Reaction system is following:
Reaction conditions: 23 ℃, 10min.Connect product transformed into escherichia coli TOP10 cell (buying Yu Tiangen company), it is dull and stereotyped to coat the LB that contains the ammonia benzyl.
Connecting product identifies:
Picking resistance bacterium colony is cultivated 12h in containing the LB liquid nutrient medium of Amp 100mg/L, extract plasmid, carries out the PCR checking, and PCR electrophoresis checking structure is seen Fig. 2.Thus, we arrive intermediate carrier pBS-T-AtDREB2A, the carrier synoptic diagram is seen Fig. 3.This carrier is sent to the order-checking of the big genome company of China, and we have obtained the base sequence of this gene, carry out sequence alignment with ClustalX2 software, and are in full accord with the sequence that Genbank is announced.
Make up plant expression vector pH7m24GW, 3-Prd29A-DREB2A-CA
Design of primers such as F1, R1 and F2, R2, sequence is seen SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8.
Use Arabidopis thaliana pBS-T-AtDREB2A to be template, respectively with F1, R1 and F2, R2 are primer, are PCR respectively with the PFU enzyme.
System is following:
Reaction conditions is:
More than two pairs of primer PCR products see the 1st and the 2nd swimming lane among Fig. 4.
Cutting glue reclaims above two kinds of fragments and does connection then:
Reaction conditions is:
So obtain connecting product.
This is connected product do the total length extension, system is following:
Reaction conditions is:
Connecting product is AtDREB2A-CA, and structure is seen the 3rd swimming lane among Fig. 4.
The PCR product is directly cut with EcoRI and EcoRV enzyme, and the enzyme system of cutting is:
37 ℃ of 12h carry out complete degestion, and enzyme is cut product and done and cut glue and reclaim, and confirms concentration, connects plant expression vector, and linked system is:
Reaction product is mixed with competent escherichia coli cell Top10, does conversion, and coats on the resistant panel that contains spectinomycin Spe, extracts plasmid, does enzyme with HindIII and cuts detection, and structure is seen Fig. 5.So obtained plant expression vector pH7m24GW, 3-Prd29A-AtDREB2A-CA, the carrier synoptic diagram is seen Fig. 6.This carrier is sent to the order-checking of the big genome company of China; We have obtained the base sequence of the conjugated protein 2A constitutive activation of Arabidopis thaliana dehydration response original paper (constitutive activation/CA) Gene A tDREB2A-CA, i.e. nucleotide sequence shown in the SEQ ID No.1.
The Agrobacterium engineering strain C58:pH7m24GW that is used for plant transgene, the structure of 3-Prd29A-DREB2A-CA.
The preparation of Agrobacterium competent cell
With the single colony inoculation of Agrobacterium C58 in 5mL YEP liquid nutrient medium, 28 ℃, the 180r/min shaking culture is spent the night;
2. above-mentioned bacterium liquid is changed in the 100mL YEP liquid nutrient medium, 28 ℃, 180r/min, shaking culture (OD
600Value is about 0.5);
3. behind the ice bath 30min, 4 ℃, the centrifugal 10min of 4000r/min collects thalline, is resuspended in the H of 20mL precooling
2Among the O;
4. 4 ℃, the centrifugal 10min of 4000r/min collects thalline, be resuspended in 10% glycerine of precooling, every pipe 200 μ L packing, liquid nitrogen flash freezer, be stored in-80 ℃ subsequent use.、
The electric shock of plant expression vector transforms
1. the C58 competent cell with-80 ℃ of taking-ups places on ice, makes its slow thawing;
2. add 2 μ L plasmids, mixing;
3. be transferred in the electric shock cup, more than operation is all carried out on ice;
4. electric shock conversion instrument parameter is set: 25 μ F, 400olm, 1500V, 5ms, electric shock transforms;
5. room temperature adds 1mL YEB liquid nutrient medium after leaving standstill 2min, and 28 ℃, 180r/min shaking culture 4h;
6. get 50 μ L bacterium liquid and coat on the YEB flat board that contains 100mg/L Ka resistance, be inverted flat board, cultivate 48h for 28 ℃, until seeing single clearly bacterium colony.
Bacterium colony PCR
1. the single bacterium colony of the some C58 of picking is dissolved in 30 μ L ddH respectively
2Among the O, 98 ℃ are boiled 10min;
2. get above-mentioned bacterium liquid 1 μ L as template, the response procedures of accordinging to above-mentioned this gene carries out the PCR reaction.
Agriculture bacillus mediated tobacco genetic transformation
The sterile culture of tobacco seedling: tobacco seed is sowed on substratum; select full, healthy tobacco seed; with peace tiformin (available chlorine 2.5%) aqueous solution sterilization 8min of 75% alcohol immersion 1min 25%, rinsed with sterile water three times is placed on seed in the MS substratum; 25 ℃ of light are cultivated the 16h/8h photoperiod.
(1) the single bacterium colony of the positive Agrobacterium of picking is inoculated in the LB liquid nutrient medium that contains the 100mg/L kantlex, incubated overnight on 28 ℃, the shaking table of 200r/min.
(2) be in vigorous period (OD when bacterium liquid
600=0.6-0.9) time, pouring bacterium liquid into the 50ml centrifuge tube, centrifugal 15min abandons supernatant under 4000r/min, collects thalline.
(3) with sterile water wash thalline 2 times.
(4) with infecting the resuspended thalline of substratum, make OD
600=0.9-1.2.
Explant is contaminated
Tobacco leaf is removed master pulse and limb edge, then blade is cut into 1cm * 1cm size, immerse the Agrobacterium bacterium liquid for preparing; Soak 8-15min, shake 2-3 time therebetween, make blade fully contact bacterium liquid; Take out blade; With the unnecessary bacterium liquid of aseptic filter paper exhaustion, the blade face down, blade back is inoculated in the common substratum up, cultivated 3-4 days about 25 ℃.
(2) blade is transferred in the screening culture medium, changes a subculture about 20 days, (Fig. 7 a) about resistant buds is from callus length to 1cm (Fig. 7 b), cuts resistant buds from callus to the generation of induction of resistance bud, is inoculated in resistance seedling rooting substratum (Fig. 7 c).
Transgenic seedling is transplanted
Root growth is good, that vitality is vigorous tobacco tissue cultured seedling takes out figure from tissue culture bottle; Wash substratum (as far as possible reducing the root system damage) with tap water; Be planted in vermiculite and the compost humous, coating film heat and moisture preserving 15 days is opened film then; Regularly water, apply fertilizer, make it normal growth in the greenhouse (Fig. 7 d).
The Molecular Detection of transgene tobacco and salt resistance detect
The PCR of transgene tobacco genomic dna detects: the 1.CTAB method is extracted the total DNA of tobacco; 2. be template with the genomic dna, carry out PCR and detect that primer is P5, P6, reaction conditions:
DREB2A detects primer
F?TGACGGTACTACTGTGGCT
R?TTCTACAATCCCTTGCTCC
The PCR reaction system:
The pcr amplification condition:
Get above-mentioned PCR product 5 microlitres, carry out electrophoresis detection, as shown in Figure 8, explain that the AtDREBA2A-CA gene successfully changes tobacco over to.
The salt resistance of transgene tobacco detects
Will be in the greenhouse normal growth, 5-7cm transgene tobacco seedling and wild-type contrast seedling are watered the water of the NaCl that contains 300mmol/L and 500mmol/L concentration respectively, in the greenhouse, keep 85% humidity.Monitoring finds that under the growth conditions of 300mmol/LNaCl, the tobacco growing of wild-type is slow, and living weight is less.So opposite, transgene tobacco can blossom and bear fruit, and living weight is higher relatively.See Fig. 9 a.Under the growth conditions of 500mmol/LNaCl, after the week of growing, the wild-type tobacco yellow leaf is wilted, and tissue necrosis can not normal growth.Although it is the growth of transgene tobacco also receives inhibition to a certain degree, far obvious unlike the wild-type tobacco that kind.See Fig. 9 b.
Claims (4)
1. the conjugated protein 2A constitutive activation of the dehydration response original paper gene (AtDREB2A-CA) in an Arabidopis thaliana source is characterized in that it is the nucleotide sequence shown in the SEQ ID No.1.
2. the protein of the conjugated protein 2A constitutive activation of the described Arabidopis thaliana dehydration response of claim 1 original paper genes encoding is characterized in that described protein is the aminoacid sequence shown in the SEQ ID No.2.
3. a recombinant vectors is characterized in that containing the described Arabidopis thaliana dehydration response of claim 1 original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) complete sequence.
4. the application of the described Arabidopis thaliana dehydration response of claim 1 an original paper conjugated protein 2A constitutive activation gene (AtDREB2A-CA) is characterized in that it is used to prepare transgene tobacco.
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| Swarbreck D et al.Genbank：NM_120623.2.《Genbank》.2011,全文. * |
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Inventor after: Ji Jing Inventor after: Wang Gang Inventor after: Zhao Qing Inventor after: Guan Chunfeng Inventor after: Cao Yueping Inventor after: Qiu Lijuan Inventor before: Ji Jing Inventor before: Wang Gang Inventor before: Zhao Qing Inventor before: Guan Chunfeng |
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