CN102329740A - Carbendazim degrading trichoderma sp. strain and preparation method thereof - Google Patents
Carbendazim degrading trichoderma sp. strain and preparation method thereof Download PDFInfo
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- CN102329740A CN102329740A CN201110287929A CN201110287929A CN102329740A CN 102329740 A CN102329740 A CN 102329740A CN 201110287929 A CN201110287929 A CN 201110287929A CN 201110287929 A CN201110287929 A CN 201110287929A CN 102329740 A CN102329740 A CN 102329740A
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- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 241001557886 Trichoderma sp. Species 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000006013 carbendazim Substances 0.000 title abstract description 9
- 230000000593 degrading effect Effects 0.000 title abstract description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 title abstract 5
- 239000002689 soil Substances 0.000 claims abstract description 22
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 230000000443 biocontrol Effects 0.000 claims abstract description 5
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- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 4
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- 208000031888 Mycoses Diseases 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 241000223259 Trichoderma Species 0.000 claims description 19
- 230000015556 catabolic process Effects 0.000 claims description 18
- 238000006731 degradation reaction Methods 0.000 claims description 18
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- 235000021307 Triticum Nutrition 0.000 claims description 10
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- 238000012216 screening Methods 0.000 claims description 6
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- 238000004519 manufacturing process Methods 0.000 claims description 4
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- 238000009629 microbiological culture Methods 0.000 abstract 1
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- QTENRWWVYAAPBI-YCRXJPFRSA-N streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](N=C(N)N)[C@H](O)[C@@H](N=C(N)N)[C@H](O)[C@H]1O QTENRWWVYAAPBI-YCRXJPFRSA-N 0.000 description 1
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Abstract
The invention discloses a carbendazim degrading trichoderma sp. strain and a preparation method thereof. A biocontrol strain provided by the invention is trichoderma sp. Tr1; the strain is preserved in the China General Microbiological Culture Collection Center; the preservation number is CGMCC No. 5210; the strain is obtained through separating, sieving and culturing from soil polluted by carbendazim by utilizing an inorganic salt culture medium containing the carbendazim; and a microbial preparation prepared by applying a zymophyte solution of the strain can be used for preventing and treating the fungal diseases of a plant and biologically repairing the soil which is polluted by the carbendazim simultaneously.
Description
Technical field
The invention belongs to biological high-tech field, it is mould to relate to a kind of derosal chemical residual degradation wood, is to utilize method of microorganism degraded chemical pesticide residual, is applicable to the production and the processing of green non-polluted farm product in the modern agriculture production.
Background technology
Derosal (Carbendazim; MBC) be a kind of systemic fungicide of broad-spectrum high-efficiency and low-toxicity; Chemical name is N-(2-benzimidazolyl-) Urethylane; Various farm crop, gourd, fruit and vegetable disease are had the better prevention effect, also are other sterilant, for example the metabolic intermediate of imidazoles such as F-1991, thiophanate_methyl sterilant.According to statistics, China 2006 agricultural chemicals total demand (effective content) is 29.96 ten thousand tons, and wherein the demand of derosal is at 0.8～1.0 ten thousand ton.Derosal character in soil and water is highly stable, and degradation half life is longer, residually in vegetables, fruit and soil can influence HUMAN HEALTH through food chain with accumulation, for example causes chromosome aberration, produces disease of the liver etc.Classified as environmental hormone class chemical pesticide by China in 2002.Therefore, the Study on degradation of derosal in environment more and more receives people's attention.
Biological degradation is to remove the residual main path of derosal in the environment, and the derosal degradation bacteria of having reported at present is the bacterium class.Existing Trichoderma class does not almost have degradation function to derosal, even in the with serious pollution soil of derosal, can't survive and breed.
Summary of the invention
The present invention seeks to the deficiency to prior art, provide biocontrol trichoderma bacterial strain that a strain has the derosal Degradation and
Preparation and application.
The biocontrol strain of a strain degradable derosal provided by the invention be wood mould (
Trichoderma sp.) Tr1, this bacterial strain is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation; Preserving number is CGMCC No.5210, and the preservation time is on September 1st, 2011, and the preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The trichoderma strain of degradable derosal of the present invention (
Trichoderma sp.) Tr1 utilizes the minimal medium contain derosal, from receive the derosal Contaminated soil, separate, screening, cultivate and obtain.This bacterial strain not only has good degradation effect to derosal, and various pathogenic bacterias are also had certain fungistatic effect.This invention is laid a good foundation for the Trichoderma goods that obtain the degraded derosal.
The biocontrol strain wood of degradable derosal of the present invention mould (
Trichoderma sp.) the separation and Culture condition of Tr1, comprise the following steps:
Gather the vineyard agric (10-30cm) of Donggang District San Zhuan town Ji Wa village long-term application derosal at sunshine; Take by weighing soil sample 10g; Be added in the minimal medium that the 100ml carbendazim concentration is 1000 μ g/ml, 28 ℃ of 150r/min shaking tables are cultivated 7d, draw the 10ml nutrient solution and are transferred in the same medium; Cultivate 7d, transfer 5 times continuously altogether.Get the 1ml nutrient solution, dilution 10
-5Get 200 μ l nutrient solutions and evenly coat solid inorganic salt culture medium (wherein add 100 μ g/ml Streptomycin sulphates and be used for suppressing bacterium), 28 ℃ are cultured to the dull and stereotyped single bacterium colony of appearance of going up, bacterial strain are transferred to PDA flat board (wherein add 100 μ g/ml Streptomycin sulphates and be used to suppress bacterium); 28 ℃ of constant temperature culture; After being purified to pure culture repeatedly, being transferred to and continuing the shaking table cultivation in the minimal medium, utilize HPLC to detect the degradation capability of bacterial strain derosal; Screening is carried out preservation to the strongest bacterial strain of derosal degradation capability, be preserving number and be CGMCC No.5210 wooden mould (
Trichoderma sp.) the Tr1 bacterial strain.
The bacterial strain that above-mentioned cultivation screening is obtained is transferred on the PDA flat board, finds this bacterial strain bacterium colony initial stage aerial hyphae fine and close clump pencil that is white in color on the PDA flat board, and the edge is loose; Be the divergent shape growth; The back side initial stage is colourless, and the later stage mycelia occurs and produces the spore district, color from light green gradually to deep green.Conidiophore bears from the mycelia side shoot, is the verticillate arrangement of cross, acroconidium, and conidium is smooth, and is inferior spherical to avette (Fig. 1).Through a series of cultivation shapes and observation by light microscope, through be accredited as Trichoderma (
Trichoderma sp.).
Above-mentioned cultivation, screening wood mould (
Trichoderma sp.) culture medium prescription of Tr1 is following:
Minimal medium (g/L): NaCl 1.0g, K
2HPO
41.5g, KH
2PO
40.5g, MgSO
47H
2O 0.2g, NH
4NO
31.0g, zero(ppm) water 1000mL, derosal (100mg/mL) 10ml.
Solid inorganic salt culture medium (g/L): add agar 10g in the above-mentioned minimal medium.
PDA substratum: yam 200g, glucose 20g, agar 15g, zero(ppm) water 1000ml.
The fermentation method for producing of wood according to the invention mould (Trichoderma sp.) Tr1 is following:
Adopt solid PDA substratum, should be seeded on the test tube substratum by the mould Tr1 of wood, behind the 27-30 ℃ of following cultivation 48h, cultured Trichoderma kind is transferred in the liquid PDA substratum, 28 ℃ of shaking tables are cultivated 72h.The trichoderma strain that liquid culture is good is transferred in the wheat broth according to the inoculum size of 1% (v/w), 27-30 ℃ of enlarged culturing 3d.Clean cultured trichoderma strain, process 7 * 10
9The wooden mould spore suspension bacteria liquid of individual/ml.
The mould spore suspension bacteria liquid of above-mentioned resulting wood can be processed the commodity microbial preparation through conventional technical process.
Above-mentioned wheat broth is for soaking, boil prepared liquid wheat broth with wheat with clear water.
Concrete formulation examples is following:
Take by weighing the 1000g wheat, after cleaning up, add 10L clear water soaked overnight, boiled in second day till do not harden, feel that with feel wheat is not clamminess 40 bottles of packing wide-mouth vials, 121 ℃, 20min autoclaving, twice simultaneously.
Use wood according to the invention mould (
Trichoderma sp.) microbial preparation that the Tr1 zymocyte liquid makes, can be used for preventing and treating fungal diseases of plants, carry out biological prosthetic to receiving the derosal Contaminated soil simultaneously.
Description of drawings
Fig. 1 is wooden mould Tr1 conidiophore and conidium
Fig. 2 is the fungistatic effect of derosal degradation bacteria Tr1 to various pathogenic bacterias.1：
Rhizoctonia?solani；2：
Phytophthora?capsici；3：
Verticillium?dahliae?Kleb；4：
Phoma?uvicola?Berk；5：
Bipolaris?sorokiniana（Sacc.）hoemaker；6：
Fusarium?moniliforme?Sheld
Fig. 3 is that wooden mould Tr1 is to containing the biological prosthetic of derosal soil.
Embodiment
Wood mould (
Trichoderma sp.) cultivation of Tr1 and derosal degradation capability detect
The mould Tr1 of wood is transferred on PDA (adding Streptomycin sulphate, the 100 μ g/ml) flat board, behind the cultivation 5D, uses the aseptic water washing spore, process 10
9Individual/the ml spore suspension, the inoculum size according to 1% is transferred in the minimal medium, and 28 ℃ of shaking tables are cultivated 14D, is contrast with the derosal degraded substratum that does not connect bacterium.Cultivation finishes, and is that the ratio of 1:4 is adding acetone according to volume ratio, behind the mixing, adopts HPLC (HPLC) to analyze.Moving phase is acetonitrile: water=18:82.Flow velocity is 1ml/min, sample size 10 μ l, and the operation wavelength of variable-wavelenght detector is 270nm, adopts external standard method to press peak area quantification, calculates the derosal degradation rate according to formula:
In the formula, X is a degradation rate, and A is not for connecing carbendazim concentration in the bacterium treatment solution, and B is for connecing carbendazim concentration in the bacterium treatment solution.
Degradation rate to derosal in this bacterial strain 14D has reached 55.47%, and the promptly 14 days 554.7mg that degraded are through condition optimizing; Inorganic nitrogen-sourced replace to organic nitrogen source (yeast powder, 1.0g/L), culture temperature is 28 ℃; Initial pH6.5, degradation rate has reached 63%, i.e. derosal about 630mg that degraded.
Embodiment 2.
The mould Tr1 bacteriostatic activity of wood detects
Various pathogenic bacterias and wooden mould Tr1 are after cultivating three days on the PDA substratum; Get the collar plate shape culture block of the silk that carries disease germs with the sterilization punch tool; The culture block that collar plate shape is had pathogenic bacteria is put apart from the dull and stereotyped central 1.5cm of PDA place; The substratum bacterium piece of the mould Tr1 of wood is placed on apart from pathogenic bacteria bacterium piece 3.0 cm places 28 ℃ of width of cultivating the antibacterial band of record after 3 days down.Calculate the bacteriostasis rate of wooden mould Tr1.In the experiment with the wooden removing mildew LTR-2 wettable powder sold on the market as contrast.
Above-mentioned pathogenic bacteria is:
Rhizoctonia solani,
Phytophthora capsici,
Verticillium dahliaeKleb,
Phoma uvicolaBerk,
Bipolaris sorokiniana(Sacc.) hoemaker,
Fusarium moniliformeSheld
The result sees Fig. 2, can be known by Fig. 2, and wooden mould Tr1 and LTR-2 all have restraining effect to a certain degree to various pathogenic bacterias,
Rhizoctonia solani,
Phytophthora capsici,
Verticillium dahliaeSeveral kinds of pathogenic bacterias of Kleb, the fungistatic effect of LTR-2 is better than Tr1, and right
Phoma uvicolaBerk,
Bipolaris sorokiniana(Sacc.) hoemaker,
Fusarium moniliformeSheld, the fungistatic effect of wooden mould Tr1 are better than wooden mould LTR-2 slightly, explain in this invention and to separate the trichoderma strain Tr1 that the obtains derosal of not only can degrading, and various plant pathogenic fungis are also had certain inhibition effect.
Embodiment 3.
Derosal degraded Trichoderma is to the biological prosthetic test of soil
Get the biological centre base soil in Shandong Scientific Research Academy, air-dry, sieve, detect the content of derosal in the soil.Find to screen in the soil that obtains to contain derosal packing, 40g/ bottle, 121 ℃ of sterilization 2h.Carry out soil treating according to table 2, after stirring, get 1g and detect derosal content.Whenever detect at a distance from sampling in 7 days later on, detected for 4 weeks altogether.Wherein derosal adopts 50% wettable powder, every processing triplicate.Adopting HPLC to detect the derosal content in the soil, is contrast not add wooden mould Tr1.
Annotate: "+", the gram number of adding is 5g; "-" do not add any material, and finally restraining number is the 50g/ bottle.
The mould Tr1 of wood sees Fig. 3 to the soil organisms repairing test result who pollutes derosal.
Can see by Fig. 3; Behind the mould Tr1 of wood and sterile soil, derosal mixing, find prolongation along with the time, the derosal content in the soil obviously reduces; Soil after wooden mould Tr1 handles detects derosal content 29.74% after 28 days, degradation rate has reached 43.96%.Explain that the wooden mould Tr1 that obtains among the present invention has played significant feature in the soil remediation test.
Claims (5)
- One strain have the derosal Degradation the biocontrol trichoderma bacterial strain ( Trichoderma sp.) Tr1, this bacterial strain is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and preserving number is CGMCC No.5210.
- Trichoderma strain 2. as claimed in claim 1 ( Trichoderma sp.) Tr1, it is characterized in that this bacterial strain is to utilize the minimal medium that contains derosal, separation from receive the derosal Contaminated soil, screening, cultivation obtain.
- Trichoderma strain 3. as claimed in claim 1 ( Trichoderma sp.) Tr1, the substratum that it is characterized in that screening, cultivate this bacterial strain is formed as follows:Minimal medium (g/L): NaCl 1.0g, K 2HPO 41.5g, KH 2PO 40.5g, MgSO 47H 2O 0.2g, NH 4NO 31.0g, zero(ppm) water 1000mL, derosal (100mg/mL) 10ml,Solid inorganic salt culture medium (g/L): add agar 10g in the above-mentioned minimal medium,PDA substratum: yam 200g, glucose 20g, agar 15g, zero(ppm) water 1000ml.
- Trichoderma strain 4. as claimed in claim 1 ( Trichoderma sp.) Tr1, it is characterized in that fermentation method for producing is following:Adopt solid PDA substratum, should wood mould ( TrichodermaSp.) Tr1 is seeded on the test tube substratum; After cultivating 48h under 27-30 ℃, cultured Trichoderma kind is transferred in the liquid PDA substratum, 28 ℃ of shaking tables are cultivated 72h; The trichoderma strain that liquid culture is good is transferred in the wheat broth according to the inoculum size of 1% (v/w); 27-30 ℃ of enlarged culturing 3d cleans cultured trichoderma strain, processes 7 * 10 9The wooden mould spore suspension bacteria liquid of individual/ml; Wherein, wheat broth is for soaking, boil prepared liquid wheat broth with wheat with clear water.
- 5. like the application of claim 1 and 4 described trichoderma strains (Trichoderma sp.) Tr1 zymocyte liquid; It is characterized in that the microbial preparation that its zymocyte liquid makes; Can be used for preventing and treating fungal diseases of plants, carry out biological prosthetic to receiving the derosal Contaminated soil simultaneously.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107541468A (en) * | 2017-08-29 | 2018-01-05 | 东北林业大学 | A kind of short close trichoderma, microbial inoculum, method and the application in degrading imazethapyr |
| CN109633026A (en) * | 2019-01-03 | 2019-04-16 | 上海市农业科学院 | A kind of method that liquid chromatography-tandem mass spectrometry detects carbendazim and its metabolin |
| CN115739973A (en) * | 2022-11-29 | 2023-03-07 | 湖南农业大学 | Method for treating carbendazim contaminated soil by combining mushroom dregs with nitrification inhibitor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575574A (en) * | 2009-06-11 | 2009-11-11 | 刘和现 | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection |
| EP2409570A2 (en) * | 2010-06-29 | 2012-01-25 | Basf Se | Fungicidal mixtures based on pyrazolopyridine compounds |
-
2011
- 2011-09-26 CN CN201110287929.3A patent/CN102329740B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101575574A (en) * | 2009-06-11 | 2009-11-11 | 刘和现 | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection |
| EP2409570A2 (en) * | 2010-06-29 | 2012-01-25 | Basf Se | Fungicidal mixtures based on pyrazolopyridine compounds |
Non-Patent Citations (4)
| Title |
|---|
| XU J L等: "Isolation and characterization of a carbendazim－degrading Rhodococcus sp． djl－6", 《CURR MICROBIOL》, 31 December 2006 (2006-12-31), pages 72 - 76 * |
| 惠有为等: "木霉在植物真菌病害防治上的作用", 《西北农业学报》, vol. 12, no. 3, 31 December 2003 (2003-12-31), pages 96 - 99 * |
| 田连生和陈菲: "多菌灵降解菌T8－2 的分离及其降解条件研究", 《江苏农业科学》, no. 6, 31 December 2008 (2008-12-31), pages 271 - 274 * |
| 黄玉杰等: "多菌灵降解菌的分离、鉴定及其降解特性研究", 《山东科学》, vol. 24, no. 2, 30 April 2011 (2011-04-30), pages 28 - 34 * |
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