CN102327292A - Refined powder of sheep embryo and sheep placenta and preparation method thereof - Google Patents
Refined powder of sheep embryo and sheep placenta and preparation method thereof Download PDFInfo
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- CN102327292A CN102327292A CN201110193933A CN201110193933A CN102327292A CN 102327292 A CN102327292 A CN 102327292A CN 201110193933 A CN201110193933 A CN 201110193933A CN 201110193933 A CN201110193933 A CN 201110193933A CN 102327292 A CN102327292 A CN 102327292A
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Abstract
The invention relates to a sheep embryo and sheep placenta biological product and a preparation method thereof. The preparation method of refined powder of the sheep embryo and sheep placenta comprises the following steps of: (1) preliminarily treating raw materials of the sheep embryo and sheep placenta; (2) carrying out ultra-fine pulverization and cell-wall breaking on the sheep embryo and sheep placenta; (3) carrying out extraction and enzymolysis on the sheep embryo and sheep placenta; and (4) carrying out vacuum concentration on a stock solution of the sheep embryo and sheep placental peptide until the relative density is 1.05-1.10 and spray drying to obtain the refined powder of the sheep embryo and sheep placenta. The refined powder of the sheep embryo and sheep placenta, which is prepared by adopting the method, not only retains natural active substances in the placenta, but also increases the contents of polypeptides and amino acid in the placenta.
Description
Technical field
The present invention relates to a kind of sheep embryo and Placenta caprae seu ovis biological product and preparation method thereof.
Background technology
China utilizes that various organs, tissue and the metabolite of animal are cured the disease, diseases prevention and health care with a long history, existing complete pharmacy ancient books and records---" legendary god of farming's book on Chinese herbal medicine opinion " just has the record of multiple animal pharmaceuticals such as Calculus Bovis, Cornu rhinocerotis, Cornu Cervi Pantotrichum, Colla Corii Asini.Explained that China utilizes animal pharmaceuticals to process, extract already, refiningly forms comparatively purified medicament on pharmaceutical technology.
Placenta Hominis (Placenta) has another name called " Placenta Hominis "; Be conventional Chinese medicine; Modern medicine study shows: Placenta Hominis contains bioactive substance such as panimmunity globulin, bioactive peptide and aminoacid, mineral matter and other components (the general designation living cells is plain), is a kind of regulator and nutrition, cosmetic health product of body's immunity.Scientist compares the experiment back to multiple animal Placenta Hominis and finds that the cell tissue structure of Placenta caprae seu ovis is the most similar with people's Placenta Hominis, is expected to become with the human placenta to have the substitute that equal health preserving is worth.
In recent years, occurred two kinds of novel Placenta caprae seu ovis biological product of animal Placenta Hominis hydrolyzed solution and Placenta Hominis lixiviating solution at home and abroad again in succession,, caused gazing at of people because of its unique usefulness.But we think that above-mentioned two kinds of preparations respectively have drawback, and the former destroys the natural active matter in the Placenta Hominis in hydrolytic process, loses the meaning of utilizing Placenta Hominis; Though the latter has multiple natural active matter, polypeptide and amino acid content are very little, and the two is combined together, have both kept the natural active matter in the Placenta Hominis, increase its polypeptide and amino acid content again.This is a new breakthrough in Foetus Caprae seu Ovis and the developmental research of Placenta caprae seu ovis biological product undoubtedly.
Summary of the invention
In order to solve above-mentioned technical problem, an object of the present invention is to provide the method for preparing of a kind of sheep embryo and Placenta caprae seu ovis refined powder, this method had both kept the natural active matter in the Placenta Hominis, increased its polypeptide and amino acid content again.Another object of the present invention provides above-mentioned sheep embryo and Placenta caprae seu ovis refined powder.
In order to realize first above-mentioned purpose, the technical scheme below the present invention has adopted:
The method for preparing of a kind of sheep embryo and Placenta caprae seu ovis refined powder, this method comprises the steps:
1) sheep embryo and the pre-treatment of Placenta caprae seu ovis raw material: getting sheep embryo and Placenta caprae seu ovis is raw material, cleans, and removes impurity, is ground into coarse powder under 0～5 ℃ of low temperature;
2) sheep embryo and Placenta caprae seu ovis micronizing, cell wall breaking: behind the freezing 1～3h of sheep embryo and Placenta caprae seu ovis coarse powder low temperature-40 ℃～-50 ℃ ,-30～-10 ℃ of micronizing, cell wall breaking reaches 0.1～10 μ m granularity;
3) extraction of sheep embryo and Placenta caprae seu ovis and enzymolysis: sheep embryo after the cell wall breaking and Placenta caprae seu ovis serosity add 3～10 times of distilled water warm macerating and extract after-filtration must filtrate A and precipitate A; Sucking filtration got hydrolyzed solution B after precipitate A adopted protease hydrolyzed; Merging filtrate A and hydrolyzed solution B get the plain stock solution of sheep embryo and Placenta caprae seu ovis;
4) the plain stock solution of sheep embryo and Placenta caprae seu ovis is through being evaporated to relative density 1.05～1.10, and spray drying gets sheep embryo and Placenta caprae seu ovis refined powder.
As preferably, sheep embryo and Placenta caprae seu ovis with 2～3 monthly ages in the above-mentioned step 1) are raw material.
As preferably, protease adopts that one or both mix in papain and the trypsin in the above-mentioned step 3).
As preferably, the use amount of protease is 0.1%～2% of a precipitate A weight in the above-mentioned step 3).Preferred as again, the use amount of protease is 0.3%～1% of a precipitate A weight in the above-mentioned step 3).
As preferably, protease adopts papain to mix for two kinds with trypsin in the above-mentioned step 3), and the temperature of enzyme digestion reaction is 40～50 ℃, and pH is 7.0～8.0, papain and tryptic enzyme proportioning 1:0.5～2.Preferred as again, the temperature of enzyme digestion reaction is 45 ℃ in the step 3), and pH is 7.5, papain and tryptic enzyme proportioning 1:1.
As preferably, spray-dired EAT is 110 ℃～125 ℃ in the above-mentioned step 4), 45 ℃～60 ℃ of leaving air temps.
In order to realize second above-mentioned purpose, sheep embryo and Placenta caprae seu ovis refined powder that the present invention also provides above-mentioned any technical scheme to prepare.
The present invention is owing to adopted above-mentioned technical scheme; This technological process of manufacture can fully extract the effective ingredient in Foetus Caprae seu Ovis and the Placenta caprae seu ovis, and the active substance of Foetus Caprae seu Ovis and Placenta caprae seu ovis is maximized the retention, and is convenient to human body again and absorbs comprehensively; Technology is advanced, reasonable, quality controllable.The sheep embryo and the Placenta caprae seu ovis refined powder that adopt said method to make had both kept the natural active matter in the Placenta Hominis, increased its polypeptide and amino acid content again.
Description of drawings
Fig. 1 is the GPC-ELSD reference colour spectrogram of sheep embryo of the present invention and Placenta caprae seu ovis refined powder.
The specific embodiment
Embodiment 1
The method for preparing of a kind of sheep embryo and Placenta caprae seu ovis refined powder, this method comprises the steps:
1) sheep embryo and the pre-treatment of Placenta caprae seu ovis raw material: sheep embryo and Placenta caprae seu ovis with 2-3 monthly age are raw material, clean, remove impurity, are ground into coarse powder under the 0-5 ℃ of low temperature.
2) sheep embryo and Placenta caprae seu ovis micronizing, cell wall breaking: with low temperature (20 ℃) micronizing (the medicinal super micron mill of WZJ100) behind sheep embryo and Placenta caprae seu ovis coarse powder freezing (40 ℃～-45 ℃) 2h, cell wall breaking reaches 0.1-10 μ m granularity.
3) extraction of sheep embryo and Placenta caprae seu ovis and enzymolysis: sheep embryo after micronizing, cell wall breaking and Placenta caprae seu ovis serosity add 6 times of distilled water warm macerating and extract after-filtration must filtrate A and precipitate A; Precipitate A with papain and trypsin digestion after sucking filtration get hydrolyzed solution B; 45 ℃ of hydrolysis temperatures; Enzyme proportioning 1g:1g, pH7.5, enzyme-to-substrate are 20mg:3g; Merging filtrate A and hydrolyzed solution B get the plain stock solution of sheep embryo and Placenta caprae seu ovis.
4) the plain stock solution of sheep embryo and Placenta caprae seu ovis is through being evaporated to relative density 1.05～1.10, and spray drying gets sheep embryo and Placenta caprae seu ovis refined powder, 110 ℃-125 ℃ of EATs, 45 ℃-60 ℃ of leaving air temps.
Adopt gel permeation chromatography-evaporat light scattering (GPC-ELSD) method to measure the molecular weight distribution of Foetus Caprae seu Ovis and Placenta caprae seu ovis biological product, formulate its quality standard.
Step is following:
1. it is plain to get sheep embryo and Placenta caprae seu ovis stock solution, refined powder or refining sheep placenta, adds an amount of pure water heating or ultrasonic dissolution, puts coldly, and standardize solution filters, and gets subsequent filtrate as need testing solution.
2. liquid-phase condition: mobile phase is pure water, flow velocity 1ml/min, 30 ℃ of column temperatures.
3. immobile phase: gel filtration chromatography post (GPC) chromatographic column Shodex OHpak SB-806 HQ, Shodex OHpak SB-805 HQ, Shodex OHpak SB-804 HQ, Shodex OHpak SB-803 HQ series connection.
4. detector: evaporative light scattering detector (ELSD), evaporating temperature: 100 ℃, atomization temperature: 80 ℃, nitrogen flow rate: 1.5L/min.
The GPC-ELSD reference colour spectrogram of sheep embryo and Placenta caprae seu ovis refined powder is as shown in Figure 1.
Embodiment 2
The method for preparing of a kind of sheep embryo and Placenta caprae seu ovis refined powder, this method enzymolysis in step 3) adopts the papain, and other technologies scheme such as embodiment 1 are said.
Claims (10)
1. the method for preparing of sheep embryo and Placenta caprae seu ovis refined powder is characterized in that this method comprises the steps:
1) sheep embryo and the pre-treatment of Placenta caprae seu ovis raw material: getting sheep embryo and Placenta caprae seu ovis is raw material, cleans, and removes impurity, is ground into coarse powder under 0～5 ℃ of low temperature;
2) sheep embryo and Placenta caprae seu ovis micronizing, cell wall breaking: behind the freezing 1～3h of sheep embryo and Placenta caprae seu ovis coarse powder low temperature-40 ℃～-45 ℃ ,-30～-10 ℃ of micronizing, cell wall breaking reaches 0.1～10 μ m granularity;
3) extraction of sheep embryo and Placenta caprae seu ovis and enzymolysis: sheep embryo after the cell wall breaking and Placenta caprae seu ovis serosity add 3～10 times of distilled water warm macerating and extract after-filtration must filtrate A and precipitate A; Sucking filtration got hydrolyzed solution B after precipitate A adopted protease hydrolyzed; Merging filtrate A and hydrolyzed solution B get the plain stock solution of sheep embryo and Placenta caprae seu ovis;
4) the plain stock solution of sheep embryo and Placenta caprae seu ovis is through being evaporated to relative density 1.05～1.10, and spray drying gets sheep embryo and Placenta caprae seu ovis refined powder.
2. the method for preparing of a kind of sheep embryo according to claim 1 and Placenta caprae seu ovis refined powder is characterized in that: sheep embryo and Placenta caprae seu ovis with 2～3 monthly ages in the step 1) are raw material.
3. the method for preparing of a kind of sheep embryo according to claim 1 and Placenta caprae seu ovis refined powder is characterized in that: protease adopts that one or both mix in papain and the trypsin in the step 3).
4. the method for preparing of a kind of sheep embryo according to claim 1 and Placenta caprae seu ovis refined powder is characterized in that: the use amount of protease is 0.1%～2% of a precipitate A weight in the step 3).
5. the method for preparing of a kind of sheep embryo according to claim 4 and Placenta caprae seu ovis refined powder is characterized in that: the use amount of protease is 0.3%～1% of a precipitate A weight in the step 3).
6. according to the method for preparing of claim 1 or 2 or 3 or 4 or 5 described a kind of sheep embryos and Placenta caprae seu ovis refined powder; It is characterized in that: protease adopts papain to mix for two kinds with trypsin in the step 3); The temperature of enzyme digestion reaction is 40～50 ℃; PH is 7.0～8.0, papain and tryptic enzyme proportioning 1:0.5～2.
7. the method for preparing of a kind of sheep embryo according to claim 6 and Placenta caprae seu ovis refined powder is characterized in that: the temperature of enzyme digestion reaction is 45 ℃ in the step 3), and pH is 7.5, papain and tryptic enzyme proportioning 1:1.
8. according to the method for preparing of claim 1 or 2 or 3 or 4 or 5 or 7 described a kind of sheep embryos and Placenta caprae seu ovis refined powder, it is characterized in that: spray-dired EAT is 110 ℃～125 ℃ in the step 4), 45 ℃～60 ℃ of leaving air temps.
9. the sheep embryo and the Placenta caprae seu ovis refined powder that prepare according to claim 1 or 2 or 3 or 4 or 5 or 7 described methods.
10. sheep embryo that method according to claim 6 prepares and Placenta caprae seu ovis refined powder.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105343128A (en) * | 2015-11-19 | 2016-02-24 | 界首市菁华科技信息咨询服务有限公司 | Processing method of sheep placenta instant powder |
| CN106466329A (en) * | 2015-08-20 | 2017-03-01 | 伊犁肖尔布拉克酒业有限责任公司 | A kind of preparation method of Placenta caprae seu oviss fermented product |
| CN106619726A (en) * | 2017-01-17 | 2017-05-10 | 浙江美姿婷化妆品有限公司 | Sheep embryo enzymolysis and extraction-isolation technique |
| CN107184609A (en) * | 2017-06-09 | 2017-09-22 | 郑州源创吉因实业有限公司 | A kind of lossless sterile bulk preparation method of Human plactnta |
| CN107216366A (en) * | 2017-07-26 | 2017-09-29 | 西藏天慈生物科技有限公司 | A kind of preparation method and applications of Goat Placenta small-molecular peptides |
| CN107549657A (en) * | 2016-06-30 | 2018-01-09 | 贺兴荣 | Goat Placenta capsule and preparation method thereof |
| CN107988298A (en) * | 2017-11-29 | 2018-05-04 | 银川伊百盛清真食品有限公司 | A kind of method of sheep placental hydrilysate extraction freeze-dried powder |
| CN108498568A (en) * | 2018-06-04 | 2018-09-07 | 沈旸 | Chinese patent drug, medical food and the preparation method of autoimmune and immune related diseases are treated with Goat Placenta or embryo |
| CN110613128A (en) * | 2019-07-24 | 2019-12-27 | 宁波华子制药设备制造有限公司 | Low-temperature extraction production process of active sheep placenta |
| CN111888322A (en) * | 2020-09-18 | 2020-11-06 | 成都清科生物科技有限公司 | Sheep placenta extracting solution with whitening function and extraction method and application thereof |
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| JPH07206888A (en) * | 1992-06-11 | 1995-08-08 | Lab Lucchini Sa | Placenta extract and its preparation |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106466329A (en) * | 2015-08-20 | 2017-03-01 | 伊犁肖尔布拉克酒业有限责任公司 | A kind of preparation method of Placenta caprae seu oviss fermented product |
| CN105343128A (en) * | 2015-11-19 | 2016-02-24 | 界首市菁华科技信息咨询服务有限公司 | Processing method of sheep placenta instant powder |
| CN107549657A (en) * | 2016-06-30 | 2018-01-09 | 贺兴荣 | Goat Placenta capsule and preparation method thereof |
| CN106619726A (en) * | 2017-01-17 | 2017-05-10 | 浙江美姿婷化妆品有限公司 | Sheep embryo enzymolysis and extraction-isolation technique |
| CN107184609A (en) * | 2017-06-09 | 2017-09-22 | 郑州源创吉因实业有限公司 | A kind of lossless sterile bulk preparation method of Human plactnta |
| CN107216366A (en) * | 2017-07-26 | 2017-09-29 | 西藏天慈生物科技有限公司 | A kind of preparation method and applications of Goat Placenta small-molecular peptides |
| CN107988298A (en) * | 2017-11-29 | 2018-05-04 | 银川伊百盛清真食品有限公司 | A kind of method of sheep placental hydrilysate extraction freeze-dried powder |
| CN108498568A (en) * | 2018-06-04 | 2018-09-07 | 沈旸 | Chinese patent drug, medical food and the preparation method of autoimmune and immune related diseases are treated with Goat Placenta or embryo |
| CN110613128A (en) * | 2019-07-24 | 2019-12-27 | 宁波华子制药设备制造有限公司 | Low-temperature extraction production process of active sheep placenta |
| CN111888322A (en) * | 2020-09-18 | 2020-11-06 | 成都清科生物科技有限公司 | Sheep placenta extracting solution with whitening function and extraction method and application thereof |
| CN111888322B (en) * | 2020-09-18 | 2022-09-13 | 成都清科生物科技有限公司 | Sheep placenta extract with whitening function and extraction method and application thereof |
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Application publication date: 20120125 Assignee: Anhui Hehua Biological Medical Technology Co., Ltd. Assignor: Huzhou Kanghaisi Biological Technology Co., Ltd. Contract record no.: 2013340000043 Denomination of invention: Refined powder of sheep embryo and sheep placenta and preparation method thereof Granted publication date: 20130213 License type: Exclusive License Record date: 20130408 |
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