CN102321522A - Cell scraper - Google Patents
Cell scraper Download PDFInfo
- Publication number
- CN102321522A CN102321522A CN201110286475A CN201110286475A CN102321522A CN 102321522 A CN102321522 A CN 102321522A CN 201110286475 A CN201110286475 A CN 201110286475A CN 201110286475 A CN201110286475 A CN 201110286475A CN 102321522 A CN102321522 A CN 102321522A
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- China
- Prior art keywords
- tube
- diameter tube
- thin
- cell
- cell scraper
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a cell scraper which comprises a hollow tube-like body, wherein the tube-like body is composed of a thin-diameter tube, a tapered tube and a thick-diameter tube; the front part of the tube-like body is provided with the thin-diameter tube, the back part is provided with the thick-diameter tube, the diameter of the thin-diameter tube is smaller than that of the thick-diameter tube, and the thin-diameter tube is connected with the thick-diameter tube through the tapered tube; the axial line of the thin-diameter tube and the axial line of the tapered tube form an obtuse angle; the tapered tube and the thick-diameter tube are coaxial; the inner cavities of the thin-diameter tube, tapered tube and thick-diameter tube are communicated to form an integral body; and both ends of the tube-like body are respectively provided with an opening, the opening on one end is positioned at the front end of the thin-diameter tube, and the opening on the other end is positioned at the tail end of the thick-diameter tube. The cell scraper disclosed by the invention is in a curved surface structure; when being used for scraping cells, the cell scraper can easily segment cell colonies and has a light damaging action on the cell colonies; when a suction head is installed on the cell scraper, the cell scraper can be used as a suction tube for transitional cells; and thus, the cell scraper is a convenient and efficient operating tool in the cell passage process.
Description
[technical field]
The present invention relates to passage scraping instrument, relate in particular to a kind of cell scraper.
[background technology]
Cell is substruction and the functional unit of organism; In the current society that life science develops rapidly, great scientific advancees such as animal cloning, stem cell, transgenic galactophore biological reactor animal, artificial tissue and organ all be unable to do without the platform that cell engineering is set up.Cell engineering is human according to the certain designed scheme, through on cell, ubcellular or tissue level, carrying out experimental implementation, obtains cell, tissue, the organ of reconstruct, and individual, creates the comprehensive biotechnology of outstanding kind and product.Aspects such as the related major technique field of current cell engineering has cell cultures, cytogamy, cell to tear open to close, karyomit(e) operation and transgenosis.Cell engineering has been penetrated into many fields such as human lives's clothing, food, lodging and transportion--basic necessities of life, like the medicine of treatment disease, prophylactic vaccine, the makeup of beauty treatment, the food of eating etc.Cell cultures is platform and the basis that cell engineering is able to commercial application, and a large amount of cells is used for producing and research just can be produced the cell engineering product through turning out.In numerous cells of cultivating, the embryonic stem cell that derives from human body early embryo is developing rapidly of human regenerative medicine and uses and injected cardiotonic drug.The hESC can be divided into all the composition cells of human body that comprise sperm and ovum under suitable inducing culture condition; Therefore be the cell source of human tissue engineering, will be for human cell, tissue, organ reproduce and transplant the seed cell that provides a large amount of.With hESC is the cell colony of representative; Also comprise the cancer cells in some research cancer drug curative effects application; The mode that in the process of cell cultures, must take to swipe makes cell mass from large to small; And split away off from cultivating vessel, and then be inoculated on the new cultivation vessel, thereby the cultivation that realizes cell is gone down to posterity.If use traditional trysinization mode, the hESC who is digested to individual cells can not be survived.
Passage scraping instrument using in the market is a cell scraper; The cell scraper that no matter is metal material or plastic material all is similar to the old-fashioned shaver that uses in the daily life; Blade thin when shaving directly cuts off at the beard root; And the beard that scrapes can gather on blade in a large number, is not come off and can further not be chopped into less beard residue, therefore; This cell scraper can only be used for swipe cell colony from cultivating vessel, and cell scraper can not cut progressively in the scraping process that big cell colony forms less cell colony and the purpose that reaches the cell colony that disperses.In addition; The function singleness of cell scraper does not have the application function of others in the passage operating process; The cell scraper that adopts metal material to make can not use the mode of spirit lamp flame disinfection to sterilize in operating process; In addition, cell scraper costs an arm and a leg as metal products, thin frangible of the blade of scraper or shortcomings such as formation breach and rust staining.
[summary of the invention]
The technical problem that the present invention will solve provides a kind of cell scraper efficiently easy and simple to handle.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopts is, a kind of cell scraper comprises the tubular body arranged inside of hollow; Tubular body arranged inside is made up of thin diameter tube, tapered tube and wide diameter tube; The front portion of tubular body arranged inside is a thin diameter tube, and the rear portion is a wide diameter tube, and the caliber of thin diameter tube is less than wide diameter tube, and thin diameter tube is connected through tapered tube with wide diameter tube; Thin diameter tube axis and tapered tube axis constitute the obtuse angle; Tapered tube is coaxial with wide diameter tube; The inner chamber of thin diameter tube, tapered tube and wide diameter tube is communicated as one; The two ends of tubular body arranged inside are provided with opening, and an end opening is positioned at the front end of thin diameter tube, and the other end opening is positioned at the end of wide diameter tube.
As preferably, also comprise suction nozzle, suction nozzle is connected with the end openings of wide diameter tube.
As preferably, the external diameter of thin diameter tube is 2-4mm.
As preferably, the external diameter of wide diameter tube is 8-12mm.
As preferably, also comprise the micropore ultra-filtration equipment; The micropore ultra-filtration equipment is located in the wide diameter tube, near the end openings place of wide diameter tube.
As preferably, the micropore ultra-filtration equipment comprises ultra-filtration membrane.
As preferably, tubular body arranged inside is made into integration with glass.
As preferably, tubular body arranged inside is made into integration with plastics.
The invention has the beneficial effects as follows:
Adopt the curved surface tube wall to contact during scraping with cell colony; Avoided cell colony accumulative drawback on the blade type scraper easily; Be easy to big cell colony is progressively cut apart the less cell colony of formation, the degree that cell colony is cut apart can be controlled artificially, and it is with low cost; Easy to use, high efficiency.
[description of drawings]
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation.
Fig. 1 is the structural representation of the embodiment of the invention 1.
Fig. 2 is the tubular body arranged inside structural representation of the embodiment of the invention 1.
Fig. 3 is the structural representation of the embodiment of the invention 2.
Among the figure, 1-thin diameter tube, 2-tapered tube, 3-wide diameter tube, 4-opening, 5-micropore ultra-filtration equipment, 6-scale mark, 7-suction nozzle.
[embodiment]
Embodiment 1:
A kind of cell scraper comprises the tubular body arranged inside and the suction nozzle of an inner chamber hollow.
In Fig. 1, the front portion of tubular body arranged inside is columniform thin diameter tube 1, and afterbody is the diameter wide diameter tube 3 big than thin diameter tube, and the external diameter of thin diameter tube is 4mm, and the external diameter of wide diameter tube is 12mm.
During as cell scraper, be to utilize the cylindrical outer wall pair cell of thin diameter tube 1 to swipe, than traditional blade-like cell scraper; The cylinder of the similar street roller of effect of cylindrical wall; Can be quickly and effectively cutting apart than the maxicell colony of swiping be formed less cell colony, and cell colony is difficult for accumulating on the blade, thereby reaches the requirement and the purpose of had digestive transfer culture; And thin diameter tube and tapered tube junction become bending, make more convenient to operate.
In use, the outside of wide diameter tube 3 can be used as handle grip.In wide diameter tube, be provided with micropore ultra-filtration equipment 5 near the wide diameter tube opening part in addition, the micropore ultra-filtration equipment is equipped with the polypropylene hollow fiber ultra-filtration membrane, can prevent that outside bacterium from infecting in the pipe from the wide diameter tube end openings.
When the operation of passage, need be in new cultivation vessel with cell transfer.As shown in Figure 2, at the end openings place of wide diameter tube suction nozzle 7 is housed, and on tube wall, is provided with the scale mark 6 that is used to measure the liquid in pipe volume.Therefore present embodiment can be accomplished cell transfer easily as common suction pipe, can save the cell manipulation instrument of other types, and is first-class like the aseptic rifle of pipettor and different model, practices thrift experimentation cost and resource thus.
The tubular body arranged inside of present embodiment is in one-step molding manufacturing with glass.Its glass material can washing lotion high temperature resistant, that can use the vitriol oil and potassium permanganate to process thoroughly clean, also be applicable to disinfection way such as high temperature steaming or flame calcination.Therefore, can after cleaning and sterilization, be repeatedly used.Use cost is cheap.
Embodiment 2:
As shown in Figure 3, present embodiment is will thick suction nozzle 7 one-body molded being made that directly connect thin layer through the pipe end openings with vinyon.Owing to can produce the sterile packed product as disposable syringe, therefore need not in wide diameter tube, to be provided with the micropore ultra-filtration equipment, its advantage is similar to disposable syringe when being to use, simple and convenient and not easily damaged.
The external diameter of thin diameter tube is 2mm, and the external diameter of wide diameter tube is 8mm.
Other structures of present embodiment are identical with embodiment 1.
Can know that by said structure present embodiment promptly can be used as cell scraper, can be used as suction pipe again.
Present embodiment also can use other plastic material (like SE) to make.
Claims (8)
1. a cell scraper is characterized in that, comprises the tubular body arranged inside of hollow, and said tubular body arranged inside is made up of thin diameter tube, tapered tube and wide diameter tube; The front portion of said tubular body arranged inside is a thin diameter tube, and the rear portion is a wide diameter tube, and the caliber of thin diameter tube is less than wide diameter tube, and thin diameter tube is connected through tapered tube with wide diameter tube; Thin diameter tube axis and tapered tube axis constitute the obtuse angle; Tapered tube is coaxial with wide diameter tube; The inner chamber of said thin diameter tube, tapered tube and wide diameter tube is communicated as one; The two ends of tubular body arranged inside are provided with opening, and an end opening is positioned at the front end of thin diameter tube, and the other end opening is positioned at the end of wide diameter tube.
2. cell scraper according to claim 1 is characterized in that, also comprises suction nozzle, and said suction nozzle is connected with the end openings of wide diameter tube.
3. cell scraper according to claim 1 is characterized in that, the external diameter of said thin diameter tube is 2-4mm.
4. cell scraper according to claim 1 is characterized in that, the external diameter of said wide diameter tube is 8-12mm.
5. cell scraper according to claim 1 is characterized in that, also comprises the micropore ultra-filtration equipment; Said micropore ultra-filtration equipment is located in the wide diameter tube, near the end openings place of wide diameter tube.
6. cell scraper according to claim 1 is characterized in that, said micropore ultra-filtration equipment comprises ultra-filtration membrane.
7. according to any one described cell scraper of claim 1-5, it is characterized in that described tubular body arranged inside is made into integration with glass.
8. according to any one described cell scraper of claim 1-5, it is characterized in that described tubular body arranged inside is made into integration with plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110286475A CN102321522A (en) | 2011-09-23 | 2011-09-23 | Cell scraper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110286475A CN102321522A (en) | 2011-09-23 | 2011-09-23 | Cell scraper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102321522A true CN102321522A (en) | 2012-01-18 |
Family
ID=45449362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110286475A Pending CN102321522A (en) | 2011-09-23 | 2011-09-23 | Cell scraper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102321522A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593250A (en) * | 2015-01-06 | 2015-05-06 | 长沙赢润生物技术有限公司 | Flexible single-cell separator and separation culture technology |
| CN108026500A (en) * | 2015-03-31 | 2018-05-11 | 兴盛生物科技股份有限公司 | Device is grown in cell culture training with integral type cell operation system |
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194199B1 (en) * | 1998-09-17 | 2001-02-27 | Michael Hoffman | Cell scraper device having blade on handles with pivotal joints |
| WO2002053698A1 (en) * | 2000-12-29 | 2002-07-11 | Cole Robert B | Spatula for biological sampling |
| EP1508612A1 (en) * | 2003-08-20 | 2005-02-23 | Becton, Dickinson and Company | Cell scraper |
| US20050106718A1 (en) * | 2003-11-18 | 2005-05-19 | Sundaravadivel Balasubramanian | Cell scraper and method of using same |
| TW200827439A (en) * | 2006-12-29 | 2008-07-01 | Ind Tech Res Inst | Aspiration device and method for cell and tissue |
| CN201161194Y (en) * | 2008-01-04 | 2008-12-10 | 广西壮族自治区妇幼保健院 | Connector bend dropping tube |
| CN201817472U (en) * | 2010-10-19 | 2011-05-04 | 上海交通大学医学院附属仁济医院 | Cell dissociation brush |
| CN202226852U (en) * | 2011-09-23 | 2012-05-23 | 安徽农业大学 | Cell scraper |
-
2011
- 2011-09-23 CN CN201110286475A patent/CN102321522A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194199B1 (en) * | 1998-09-17 | 2001-02-27 | Michael Hoffman | Cell scraper device having blade on handles with pivotal joints |
| WO2002053698A1 (en) * | 2000-12-29 | 2002-07-11 | Cole Robert B | Spatula for biological sampling |
| EP1508612A1 (en) * | 2003-08-20 | 2005-02-23 | Becton, Dickinson and Company | Cell scraper |
| US20050106718A1 (en) * | 2003-11-18 | 2005-05-19 | Sundaravadivel Balasubramanian | Cell scraper and method of using same |
| TW200827439A (en) * | 2006-12-29 | 2008-07-01 | Ind Tech Res Inst | Aspiration device and method for cell and tissue |
| CN201161194Y (en) * | 2008-01-04 | 2008-12-10 | 广西壮族自治区妇幼保健院 | Connector bend dropping tube |
| CN201817472U (en) * | 2010-10-19 | 2011-05-04 | 上海交通大学医学院附属仁济医院 | Cell dissociation brush |
| CN202226852U (en) * | 2011-09-23 | 2012-05-23 | 安徽农业大学 | Cell scraper |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593250A (en) * | 2015-01-06 | 2015-05-06 | 长沙赢润生物技术有限公司 | Flexible single-cell separator and separation culture technology |
| CN108026500A (en) * | 2015-03-31 | 2018-05-11 | 兴盛生物科技股份有限公司 | Device is grown in cell culture training with integral type cell operation system |
| US11332705B2 (en) | 2015-03-31 | 2022-05-17 | Thrive Bioscience, Inc. | Cell culture incubators with integrated cell manipulation systems |
| US11920122B2 (en) | 2015-03-31 | 2024-03-05 | Thrive Bioscience, Inc. | Cell culture incubators with integrated cell manipulation systems |
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
| CN114149900B (en) * | 2021-11-30 | 2024-03-29 | 中国科学院天津工业生物技术研究所 | Cutting head and mycelium cutting method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120118 |