CN102311917A - Air airborne microbe sampling method - Google Patents
Air airborne microbe sampling method Download PDFInfo
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- CN102311917A CN102311917A CN 201010215769 CN201010215769A CN102311917A CN 102311917 A CN102311917 A CN 102311917A CN 201010215769 CN201010215769 CN 201010215769 CN 201010215769 A CN201010215769 A CN 201010215769A CN 102311917 A CN102311917 A CN 102311917A
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Abstract
The invention discloses an air airborne microbe sampling method, which is characterized by providing a liquid culture medium and an air sampling tube used for air airborne microbe sampling, arranging the liquid culture medium and the air sampling tube in a sampling container, introducing the air sample into the liquid culture medium through the air sampling tube, and catching the air airborne microbe through the liquid culture medium. The air airborne microbe can be efficiently, completely and accurately acquired in the method disclosed by the invention.
Description
Technical field
The present invention relates to aerial bacterioplankton Sampling techniques field, be specifically related to a kind of aerial bacterioplankton method of sampling.
Background technology
Current, in pharmaceutical industry, biotechnology in the multiple fields such as food_beverage industry and health care, all need sample, cultivate and detect aerial bacterioplankton.
Prior art adopts the bump method to realize the sampling of aerial bacterioplankton usually.The principle of this method is to utilize off-gas pump to make the air in tested zone arrive media surface through micropore or slit high-speed impact; Small weight and the small viscosity of media surface inherent through mikrobe self are caught the mikrobe particle that part is lived; After cultivating, form bacterium colony, detect then.
Yet, can only catch the part mikrobe through this method, not only collecting efficiency is not high, and the mikrobe of being gathered can not complete and accurate ground the interior microbe population of the reflection institute air capacity of gathering.Therefore, the shortcoming of prior art is that collecting efficiency is low, and integrity and accuracy are relatively poor.
Summary of the invention
For solving the problems of the technologies described above, we have proposed a kind of aerial bacterioplankton method of sampling, to realize that aerial bacterioplankton is carried out high-level efficiency, the collection of complete and accurate ground.
For achieving the above object, technical scheme proposed by the invention is following:
A kind of aerial bacterioplankton method of sampling is characterized in that, comprising: provide the aerial bacterioplankton sampling with liquid nutrient medium and air collecting pipe; Said liquid nutrient medium and air collecting pipe are placed in the sampling container; Air sample is fed said liquid nutrient medium through said air collecting pipe, catch said aerial bacterioplankton through said liquid nutrient medium.
Preferably, also comprise, the said air sample that feeds in the said liquid nutrient medium is discharged in said sampling container.Preferably, also comprise after placing said liquid nutrient medium and air collecting pipe in the sampling container said: said sampling container is carried out high-temperature sterilization handle.
Preferably, said sampling container is a sealed vessel.
Preferably, said air collecting pipe is specially aerating pipe.
Preferably, said liquid nutrient medium comprises Carnis Bovis seu Bubali cream nutrient agar and potato culture.
Through technique scheme, the present invention feeds liquid nutrient medium with air sample, makes the bacterium that swims in the air sample by a large amount of being adsorbed in the liquid nutrient medium, thus can realize to aerial bacterioplankton carry out high-level efficiency, complete and accurate ground is gathered.
Description of drawings
Fig. 1 is the synoptic diagram of the application apparatus of the disclosed a kind of aerial bacterioplankton method of sampling of the embodiment of the invention.
Embodiment
To combine the accompanying drawing in the embodiment of the invention below, the technical scheme in the embodiment of the invention is carried out clear, intactly description, obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills are not making the every other embodiment that is obtained under the creative work prerequisite, all belong to the scope of the present invention's protection.
The invention provides a kind of aerial bacterioplankton method of sampling, to realize that aerial bacterioplankton is carried out high-level efficiency, the collection of complete and accurate ground.
In the present invention, the liquid nutrient medium that is adopted can be industrial production substratum such as Carnis Bovis seu Bubali cream nutrient agar, potato culture, root nodule bacterium substratum.The air collecting pipe that is adopted can be used the rubber hose of selling on the market, and the present invention uses the aerating pipe with micropore, makes air from each micropore, infiltrate liquid nutrient medium through aerating pipe, can increase the contact area of air and liquid nutrient medium.
The disclosed aerial bacterioplankton method of sampling of the present invention should be accomplished in encloses container, and operating process should meet aseptic requirement.
The device of the employed realization aerial bacterioplankton method of sampling can be device as shown in Figure 1 among the present invention, and is not limited to following apparatus.As shown in Figure 1; This device comprises: container bottle 1, sealing cover 2, air collecting pipe 3, Venturi meter 4, escape pipe 5, water eliminator 6 and level scale table 7; Wherein, Air collecting pipe 3 comprises inlet mouth 31, inlet pipe 32, air cavity 33 and aerating pipe 34, and an end of Venturi meter 4 is connected the below of inlet mouth 31, and the other end is positioned at the bottom of container bottle near container bottle.Air cavity 33 and aerating pipe 34 are positioned at the bottom of container bottle equally, and this structure can improve the capture rate of aerial bacterioplankton greatly.Level scale table 7 is used for the liquid level of tracer liquid substratum, and liquid level should be higher than aerating pipe 34 at least.This device in use; At first want the stopping property of inspection units; Through devices such as air pumps air sample is fed from inlet mouth 31 then; Gas gets into liquid nutrient medium through devices such as inlet pipe 32, air cavity 33 and aerating pipes 34, after the liquid nutrient medium washing, is discharged by escape pipe 5, and escape pipe 5 places can be connected to the pump housing of bleeding.Because aerating pipe 34 is provided with the many places micropore, air can produce a large amount of small bubbles during through liquid nutrient medium, makes the airborne bacterium that swims by liquid nutrient medium absorption and catch.
A kind of aerial bacterioplankton method of sampling proposed by the invention can be implemented through following manner:
The devices such as liquid nutrient medium and air collecting pipe of aerial bacterioplankton sampling usefulness at first, are provided.
Liquid nutrient medium can be industrial production substratum such as Carnis Bovis seu Bubali cream nutrient agar, potato culture, root nodule bacterium substratum.Air collecting pipe can be used the rubber hose of selling on the market; With vulcanite Guan Weijia; The rubber hose bottom should be provided with the many places aperture; Also can directly adopt aerating pipe, make air from each micropore, infiltrate liquid nutrient medium, can increase the contact area of air and liquid nutrient medium through aerating pipe with micropore.
Then, the sample devices such as liquid nutrient medium and air collecting pipe of usefulness of aerial bacterioplankton are placed a sampling container, this sampling container is a sealed vessel, and this container is carried out high-temperature sterilization handle.
Through the pump housing air sample is pumped to inlet pipe by the inlet mouth of air collecting pipe; Get into liquid nutrient medium through air cavity, aerating pipe; And produce a large amount of bubbles, after the liquid nutrient medium washing, the bacterium that swim a large amount of in the air are caught by liquid nutrient medium absorption; Gas after being washed is discharged via escape pipe 5, thereby has realized the sampling of aerial bacterioplankton.
After sampling finishes, be sealed into gas port and air outlet, to cultivate and to detect.
The present invention feeds liquid nutrient medium with air sample, makes the bacterium that swims in the air sample by a large amount of being adsorbed in the liquid nutrient medium, thus can realize to aerial bacterioplankton carry out high-level efficiency, complete and accurate ground is gathered.
To the above-mentioned explanation of the disclosed embodiments, make this area professional and technical personnel can realize or use the present invention.Multiple modification to these embodiment will be conspicuous concerning those skilled in the art, and defined General Principle can realize under the situation that does not break away from the spirit or scope of the present invention in other embodiments among this paper.Therefore, the present invention will can not be restricted to these embodiment shown in this paper, but will meet and principle disclosed herein and features of novelty the wideest corresponding to scope.
Claims (6)
1. an aerial bacterioplankton method of sampling is characterized in that, comprising:
Provide the aerial bacterioplankton sampling with liquid nutrient medium and air collecting pipe;
Said liquid nutrient medium and air collecting pipe are placed in the sampling container;
Air sample is fed said liquid nutrient medium through said air collecting pipe, catch said aerial bacterioplankton through said liquid nutrient medium.
2. method according to claim 1 is characterized in that, also comprises, the said air sample that feeds in the said liquid nutrient medium is discharged in said sampling container.
3. method according to claim 1 is characterized in that, also comprises after placing said liquid nutrient medium and air collecting pipe in the sampling container said: said sampling container is carried out high-temperature sterilization handle.
4. method according to claim 1 is characterized in that, said sampling container is a sealed vessel.
5. method according to claim 1 is characterized in that said air collecting pipe is specially aerating pipe.
6. method according to claim 1 is characterized in that said liquid nutrient medium comprises Carnis Bovis seu Bubali cream nutrient agar and potato culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010215769 CN102311917B (en) | 2010-07-01 | 2010-07-01 | Air airborne microbe sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010215769 CN102311917B (en) | 2010-07-01 | 2010-07-01 | Air airborne microbe sampling method |
Publications (2)
| Publication Number | Publication Date |
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| CN102311917A true CN102311917A (en) | 2012-01-11 |
| CN102311917B CN102311917B (en) | 2013-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010215769 Active CN102311917B (en) | 2010-07-01 | 2010-07-01 | Air airborne microbe sampling method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335952A (en) * | 2013-07-12 | 2013-10-02 | 苏州工业园区鸿基洁净科技有限公司 | On-line planktonic microorganism detector |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2498166Y (en) * | 2001-08-07 | 2002-07-03 | 常俊山 | Bottle for determining bacteria in air |
| CN1400314A (en) * | 2001-08-07 | 2003-03-05 | 常俊山 | Determination method of aerial bacterioplankton and its special-purpose determination bottle |
| US20040152150A1 (en) * | 2001-11-16 | 2004-08-05 | Gideon Eden | Detecting airborne microorganisms |
| CN101348827A (en) * | 2008-07-29 | 2009-01-21 | 常俊山 | Air planktonic bacteria detection by scrubbing method and special detecting bottle therefor |
-
2010
- 2010-07-01 CN CN 201010215769 patent/CN102311917B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2498166Y (en) * | 2001-08-07 | 2002-07-03 | 常俊山 | Bottle for determining bacteria in air |
| CN1400314A (en) * | 2001-08-07 | 2003-03-05 | 常俊山 | Determination method of aerial bacterioplankton and its special-purpose determination bottle |
| US20040152150A1 (en) * | 2001-11-16 | 2004-08-05 | Gideon Eden | Detecting airborne microorganisms |
| CN101348827A (en) * | 2008-07-29 | 2009-01-21 | 常俊山 | Air planktonic bacteria detection by scrubbing method and special detecting bottle therefor |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335952A (en) * | 2013-07-12 | 2013-10-02 | 苏州工业园区鸿基洁净科技有限公司 | On-line planktonic microorganism detector |
| CN103335952B (en) * | 2013-07-12 | 2017-07-18 | 苏州工业园区鸿基洁净科技有限公司 | Online planktonic microorganism detector |
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| Publication number | Publication date |
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| CN102311917B (en) | 2013-07-10 |
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Address after: 215000 Jiangsu Province, Suzhou City Industrial Park Loufeng venture industrial square No. 15A building Patentee after: Suzhou Hongji clean Polytron Technologies Inc Address before: 215000, No. 83, Star Road, Suzhou Industrial Park, Suzhou, Jiangsu, China Patentee before: Suzhou Industrial Park HJClean Technology Co., Ltd. |
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