CN102305732A - Method and device for discharge sampling of ship ballast water - Google Patents
Method and device for discharge sampling of ship ballast water Download PDFInfo
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- CN102305732A CN102305732A CN201110131104A CN201110131104A CN102305732A CN 102305732 A CN102305732 A CN 102305732A CN 201110131104 A CN201110131104 A CN 201110131104A CN 201110131104 A CN201110131104 A CN 201110131104A CN 102305732 A CN102305732 A CN 102305732A
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Abstract
The invention provides a method and a device for discharge sampling of ship ballast water. By the method and the device, ballast water can be collected conveniently and continuously and living aquatic organisms in the ballast water can be quickly detected. The method for the discharge sampling of the ship ballast water comprises the following steps of: arranging a discharge sampling device of the ship ballast water at a discharge water outlet of the ballast water, opening a valve, and closing the valve and taking the discharge sampling device down when the water flow reaches 1m<3>; and finally, identifying living organisms in the discharge sampling device. By the method and the device, the ballast water can be easily and continuously collected, and the living aquatic organisms in the ballast water can be quickly detected.
Description
Technical field
The present invention relates to the ballast water for ship detection range, specifically, relate to the discharging sampling method and the device of ballast water for ship.
Background technology
For guaranteeing the stability of hull when navigating by water, on the freighter of conveying crude oil or container etc., be provided with ballast box.Usually when not loading former wet goods, be full of ballast box, when loading crude oil or container etc., discharge Ballast Water, regulate the buoyancy of hull thus, make hull stable with Ballast Water.
, in Ballast Water, sneak into the hydrobiont (containing microorganism or bacterium) that survives in its bay, these hydrobionts can be transported to foreign country along with the transportation of boats and ships.Thus, thus can cause original in this marine site non-existent species invade original ecosystem constitutes competition treat etc. to the autochthons species of both having deposited ecological disruption situation.
In this case, according to " control of ballast water for ship and sediment and management international convention (draft) ", the operative norm of D-2 bar Ballast Water, the discharge capacity of carrying out living body biological in the ballast water for ship of Ballast Management is set to limit value.Article one regulation wherein: individual bulk concentration<10 of living body biological minimum dimension>=50um/m
3, the living body biological minimum dimension is individual bulk concentration<10/mL of 10~50um.
Through discovering, the individual bulk concentration of living body biological minimum dimension>=50um is extremely low in the Ballast Water, is the reliability of guaranteeing to detect, according to the suggestion of IMO, and the minimum 1m that gets of each kind
3Ballast Water, and existing boats and ships are difficult to carry out a large amount of Ballast Water samplings.
Existing ballast tank of ship is not thought of as the Ballast Water sampling and reserves sample tap at the beginning of design.Through investigating on inspection information and the ship, confirm that the collection approach of ballast water for ship has 7 kinds: fire hydrant, discharge of ballast water pipeline, Ballast Water hand-hole, discharge of ballast water mouth, sounding pipe, air hole and holdhatch.That wherein, can realize the sampling of a large amount of Ballast Waters has fire hydrant, discharge of ballast water pipeline, Ballast Water hand-hole, discharge of ballast water mouth air hole and a holdhatch.For some older boats and ships, fire main and ballast line are normally shared, and therefore available fire pump is taked Ballast Water, but new boats and ships mostly with two circuits separately, so should can only partly be suitable for by the sampling approach.The possible sampling spot of discharge of ballast water pipeline is in the cabin, and the space is narrow and small, and operation is difficult, and discarded water sample can arrange nowhere, and is actual infeasible.The discharge of ballast water mouth that also has a lot of boats and ships will be rented canoe below load line, can operate very inconvenient in the reality.Through the air hole sampling, need the cap head is put down, and when altitude above deck surpasses the 7M left and right sides, (often surpass in the reality) at the ballast tank water surface, just cannot take a sample.Holdhatch also is that few freighter is used freighter and loaded Ballast Water, unlikelyly basically in the reality runs into.
So the comparatively feasible approach of taking a large amount of Ballast Waters is through the sampling of Ballast Water hand-hole, but this needs close fit on the ship, and owing to some inlet can be covered by goods, so often be difficult to the water sample in the optimal ballast tank in the reality.And the inspection of hydrobiological survival number requires the microscopic examination Ballast Water; Concentrate under the state that must hydrobiont be lived in by Ballast Water; This concentrates operation and expends time in and manpower, therefore, accomplishes the inspection and the difficulty thereof of Ballast Water at short notice.
Summary of the invention
Technical matters to be solved by this invention is that the ballast water for ship that overcomes prior art discharges unworkable defective when taking a sample, and provides a kind of ballast water for ship discharging check point is carried out water sample sampling method efficiently.
Another technical matters to be solved by this invention is to provide a kind of discharging sampler that is used for ballast water for ship of easy, easy operating.
In order to address the above problem, technical scheme of the present invention is such: the discharging sampling method of ballast water for ship, it is characterized in that, and comprise the steps:
A, at discharge of ballast water water delivering orifice place, it is the 50um plankton net that an aperture is set, described plankton net liner aperture is the 50um bolting silk; Said bolting silk open upper end place combines with the plankton net upper end, and opening is established in the bolting silk bottom, with plankton net lower end spacing distance, forms the discharging sampler;
B. open valve, when discharge reaches 1m
3The time, close flowing water Valve, take off above-mentioned discharging sampler;
C. adopt alkaline vital stain to identify the living body biological in the discharging sampler.
Preferably, install one at described water delivering orifice place in order to detect the water flow meter of discharge.
Preferably, described alkaline vital stain is: one of dimethyl diaminophenazine chloride, janus green B, methine orchid, toluidine blue or bright cresyl viollet.
Preferably, described alkaline vital stain is the janus green B dyestuff.
Be used for the discharging sampler of ballast water for ship, it is characterized in that: at discharge of ballast water water delivering orifice place, it is the 50um plankton net that an aperture is set, and described plankton net liner aperture is the 50um bolting silk; Said bolting silk open upper end place combines with the plankton net upper end, and opening is established in the bolting silk bottom, with plankton net lower end spacing distance, forms the discharging sampler.
Preferably, between described bolting silk funnel bottom and the plankton net lower end distance less than 1cm.
Preferably, the aperture of described plankton net is 50um, and described bolting silk aperture is 50um.
Preferably, said bolting silk open upper end place and plankton net upper end are sewed up.
Annotate: according to " ballast water for ship and sediment control and management international convention (draft) ", D-2 bar Ballast Water operative norm.Be set to limit value according to this discharge capacity of carrying out living body biological in the ballast water for ship of Ballast Management.Article one regulation wherein: individual bulk concentration<10 of living body biological minimum dimension>=50um/m
3, the living body biological minimum dimension is individual bulk concentration<10/mL of 10~50um.Therefore, bolting silk net of the present invention and plankton net all adopt the 50um aperture.Limit the individual size of the planktonic organism of being gathered with this.
Adopt the main effect of bolting silk for solving the too big problem of water delivering orifice water flow pressure.Current are excessive, and planktonic organism is prone to leak outside or be crashed to pieces.Cause to occur error in the counting process, with the accuracy that reduces testing result.
Ballast water for ship discharging sampling method provided by the invention and ballast water for ship discharging sampler, the easy and continuous acquisition Ballast Water of ability, and fast detecting goes out the live body hydrobiont in the Ballast Water.
Description of drawings
Fig. 1 is whole structure figure of the present invention.
Fig. 2 is the special-purpose discharging of a ballast water for ship sampler.
Label among the figure:
1 is that ballast water for ship special-purpose discharging sampler 2 is flowmeter
3 is that 50um plankton net 4 is the 50um bolting silk
5 is that plankton net upper end annulus portion 6 is the funnel bottom of bolting silk
Embodiment
In order to make technological means of the present invention, creation characteristic, to reach purpose and effect and be easy to understand and understand, the present invention is done further elaboration below in conjunction with embodiment.
Like Fig. 2 is the special-purpose discharging of ballast water for ship sampler, and the outside is a 50um plankton net 3 for the aperture, inboard for the aperture is the 50um bolting silk, and bolting silk 4 forms funnel type, and the shop is against plankton net 3 inboards.The bolting silk 4 open upper end places of the special-purpose discharging of ballast water for ship sampler are sewed up with the upper end annulus portion 5 of plankton net 3, and opening is established in the funnel bottom 6 of bolting silk 4, and between plankton net 3 lower ends apart from less than 1cm.
Embodiment
A kind of ballast water for ship discharging sampling method comprises the steps:
(1) make the discharging sampler that is used for ballast water for ship:
At discharge of ballast water water delivering orifice place, it is 50um plankton net (buying in Wuhan Hengling Technology Co., Ltd.) that an aperture is set, and is 50um bolting silk (buying a beautiful gem fine jade commerce and trade company limited in Shanghai) in described plankton net liner aperture; Said bolting silk open upper end place and plankton net upper end are sewed up, and opening is established in the bolting silk bottom, with plankton net lower end spacing distance, form the discharging sampler;
(2) open valve, when discharge reaches 1m
3The time, close flowing water Valve, take off above-mentioned discharging sampler;
(3) identify the living body biological in the discharging sampler with janus green B dyestuff (purchase), comprise step in the high letterization glass in Shanghai Instr Ltd.:
A. will discharge in the sampler all animal planktons takes out and places specimen bottle;
B. take by weighing the 1g janus green B and be dissolved in 100ml woods Ge Shi (Ringer) solution, be heated to 30 ℃-40 ℃ a little, make it dissolving, behind filter paper filtering, be 1% janus green B solution (general existing) with join at present, to keep its abundant oxidability; During use, in every 60ml water sample, drip 1% janus green B solution 3ml;
C. dye behind the 30min, in sample, drip a small amount of formalin 5% concentration solution and fix;
D. above-mentioned sample is shaken up, sample thief 1ml observes down in the 10X mirror behind the 5min: live body animal plankton tool mitochondria position is more shallow blue-green, and all the other positions keep original body colour, and the dead volume animal plankton whole body almost all is mazarine; Can extrapolate the planktonic organism in the unit volume with this.
More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; The present invention is not restricted to the described embodiments; That describes in the foregoing description and the instructions just explains principle of the present invention; The present invention also has various changes and modifications under the prerequisite that does not break away from spirit and scope of the invention, and these variations and improvement all fall in the scope of the invention that requires protection.
Claims (8)
1. the discharging sampling method of ballast water for ship is characterized in that, comprises the steps:
A, at discharge of ballast water water delivering orifice place, it is the 50um plankton net that an aperture is set, described plankton net liner aperture is the 50um bolting silk; Said bolting silk open upper end place combines with the plankton net upper end, and opening is established in the bolting silk bottom, with plankton net lower end spacing distance, forms the discharging sampler;
B. open valve, when discharge reaches 1m
3The time, close flowing water Valve, take off above-mentioned discharging sampler;
C. adopt alkaline vital stain to identify the living body biological in the discharging sampler.
2. the discharging sampling method of ballast water for ship according to claim 1 is characterized in that, installs one at described water delivering orifice place in order to detect the water flow meter of discharge.
3. the discharging sampling method of ballast water for ship according to claim 1 is characterized in that, described alkaline vital stain is: one of dimethyl diaminophenazine chloride, janus green B, methine orchid, toluidine blue or bright cresyl viollet.
4. the discharging sampling method of ballast water for ship according to claim 1 is characterized in that, described alkaline vital stain is the janus green B dyestuff.
5. be used for the discharging sampler of ballast water for ship, it is characterized in that: at discharge of ballast water water delivering orifice place, it is the 50um plankton net that an aperture is set, and described plankton net liner aperture is the 50um bolting silk; Said bolting silk open upper end place combines with the plankton net upper end, and opening is established in the bolting silk bottom, with plankton net lower end spacing distance, forms the discharging sampler.
6. the discharging sampler that is used for ballast water for ship according to claim 5 is characterized in that, distance is less than 1cm between described bolting silk funnel bottom and the plankton net lower end.
7. the discharging sampler that is used for ballast water for ship according to claim 5 is characterized in that the aperture of described plankton net is 50um, and described bolting silk aperture is 50um.
8. the discharging sampler that is used for ballast water for ship according to claim 5 is characterized in that, said bolting silk open upper end place and plankton net upper end are sewed up.
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104140947A (en) * | 2014-05-19 | 2014-11-12 | 山东恒诚检测科技有限公司 | Method for separating living cells in sediments of ship ballast tank |
CN106442488A (en) * | 2016-08-31 | 2017-02-22 | 威海中远造船科技有限公司 | Method for detecting living organisms with size of more than 50 micrometers in ballast water |
CN109357922A (en) * | 2018-10-26 | 2019-02-19 | 珠江水利委员会珠江水利科学研究院 | A kind of body before zooplankter sample identification restores preprocess method |
CN111257050A (en) * | 2020-03-05 | 2020-06-09 | 上海海洋大学 | Ship ballast water biological collection device and use method thereof |
CN111413479A (en) * | 2020-04-14 | 2020-07-14 | 诸暨市金桥实业有限公司 | Water quality detection method and system |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104140947A (en) * | 2014-05-19 | 2014-11-12 | 山东恒诚检测科技有限公司 | Method for separating living cells in sediments of ship ballast tank |
CN104140947B (en) * | 2014-05-19 | 2016-08-24 | 山东恒诚检测科技有限公司 | The separation method of active somatic cell in a kind of ballast tank of ship deposit |
CN106442488A (en) * | 2016-08-31 | 2017-02-22 | 威海中远造船科技有限公司 | Method for detecting living organisms with size of more than 50 micrometers in ballast water |
CN106442488B (en) * | 2016-08-31 | 2019-12-10 | 威海中远造船科技有限公司 | method for detecting living organisms with size of more than 50 mu m in ballast water |
CN109357922A (en) * | 2018-10-26 | 2019-02-19 | 珠江水利委员会珠江水利科学研究院 | A kind of body before zooplankter sample identification restores preprocess method |
CN109357922B (en) * | 2018-10-26 | 2021-01-05 | 珠江水利委员会珠江水利科学研究院 | Organism recovery pretreatment method before identification of zooplankton sample |
CN111257050A (en) * | 2020-03-05 | 2020-06-09 | 上海海洋大学 | Ship ballast water biological collection device and use method thereof |
CN111413479A (en) * | 2020-04-14 | 2020-07-14 | 诸暨市金桥实业有限公司 | Water quality detection method and system |
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Application publication date: 20120104 |