CN102302801B - Silk-fibroin-coated polypropylene mesh and preparation method thereof - Google Patents
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Abstract
The invention relates to a silk-fibroin-coated polypropylene mesh as well as a preparation method and application thereof. The silk-fibroin-coated polypropylene mesh is prepared by coating the surface of a polypropylene mesh used as a support with a silk fibroin coating liquid to form a layer of silk fibroin coating; and the pores of the prepared polypropylene mesh are open. The modification process of the silk-fibroin-coated polypropylene mesh is simple and feasible, and does not use any toxic and harmful reagents; on the one hand, the tensile property of the repaired part is enhanced by utilizing the characteristics that the polypropylene mesh has high porosity and surrounding tissues can well grow; and on the other hand, the silk-fibroin-coated polypropylene mesh can be applied in clinical female pelvic floor reconstruction surgery by utilizing the characteristics that the regenerated silk fibroin has biocompatibility and can promote the growth of mechanocytes, thereby accelerating tissue growth and reducing postoperative complications.
Description
Technical field
The present invention relates to a kind of organizational project and medical science articles for use field, relate in particular to a kind of silk-fibroin-coated polypropylene mesh and its preparation method and application.
Background technology
At present, for female pelvic organ prolapse (the pelvic floor prolapse that at the bottom of basin, damage and functional deterioration cause, POP) and stress incontinence (stress urinary incontinence, be called for short: SUI) etc. the treatment of disease is general adopts traditional surgical intervention scheme, by operation, carries out the repairing of vagina front and rear wall and uterectomy.Yet above-mentioned two kinds of therapeutic schemes can only be repaired the female pelvic organ of prolapsus, and cannot improve the intensity of tissue itself, cannot reach anatomical structure at the bottom of basin and rebuild, thereby thoroughly cure the object of the diseases such as female pelvic organ prolapse, therefore Postoperative recurrent rate is high.In recent years, in order to overcome tradition for the deficiency in the female pelvic organ of surgical intervention reparation prolapsus, the polymer fabric sheet material that polypropylene mesh is representative is widely used at the bottom of basin in reconstruction operations.These polymer fabric sheet material porositys are higher, and tissue merges better, has good biomechanical property, thereby can effectively improve the intensity of tissue itself.Adopt the Postoperative recurrent rate of these synthetic net sheet material to be starkly lower than kposthesis at the bottom of traditional basin.
Yet, adopt polymer fabric sheet material can effectively treat the diseases such as organ comes off at the bottom of basin, but the biocompatibility of above-mentioned polymer fabric sheet is poor, after implantation, thereby may cause such as a series of complication relevant to net sheet such as corroding exposure, infection, pain, sexual intercourse discomfort, affect the quality of life after operation in patients, the especially erosion of polypropylene mesh, often relevant with the histocompatibility of implanting polypropylene mesh, and need again perform the operation and remove net sheet, to patient, bring misery.
In order to overcome above-mentioned polymer fabric sheet, after human body is implanted, easily produce the problems such as complication, thereby people begin to demand and carry out at polymer fabric sheet the material behavior that finishing improves polymer fabric sheet, improve its biocompatibility.At present the method for net sheet material transformation is had: collagen covering, fatty acid coating, polyvinylidene fluoride coating and metallic element titanium coating etc.Wherein, what effect was best is collagenic coating, and it can improve histocompatibility, reduces inflammatory reaction and tissue fibering, promotes collagen formation etc.But because the factors such as the separating and purifying technology of collagenic coating is complicated, and the early-stage preparations time is longer, expensive restrict its extensive use.
Afterwards, state, inside and outside scholar, in the human body cell strain to different, in the increment study on silk protein membrane, find: fibroin is the same with collagen protein, can promote the growth of cell.
Fibroin is a kind of natural structure albumen, and with respect to collagen protein, it has, and source is wide, and price is low, and preparation is simple, and the advantage such as it is better to possess equally biocompatibility, and complication rate is low.Fibroin albumen is comprised of 18 seed amino acids, and wherein main have three kinds of simple aminoacid: glycine, and alanine and serine form, and they approximately occupy 85% of Tot Prot.In fibroin, there are a lot of hydrophilic amino acid residues, the biocompatibility that it is good and nontoxic, it is increasingly extensive in the application of medical field that nonirritant makes it, as medical fibroin protein skin regeneration film, cell culture medium, medicine is controlled carrier, medical dressing, tissue engineering bracket material etc.But up to now, there is no the relevant report that silk-fibroin-coated polypropylene mesh is applied in reconstructive surgery at the bottom of department of obstetrics and gynecology basin both at home and abroad.
Summary of the invention
The invention provides a kind of silk-fibroin-coated polypropylene mesh with and preparation method thereof, its defect that embedded material used exists in reconstructive surgery at the bottom of for above-mentioned existing department of obstetrics and gynecology basin, surface-coated one deck fibroin coating at polypropylene mesh, thereby increase the biocompatibility of polypropylene mesh, avoid the generation of the complication that polymer fabric sheet is relevant.
A kind of silk-fibroin-coated polypropylene mesh of the present invention and preparation thereof and application are achieved through the following technical solutions its object:
A silk-fibroin-coated polypropylene mesh, wherein, comprises the polypropylene mesh as support; On described polypropylene mesh surface, adopt fibroin coating solution parcel one deck fibroin coating; And the hole of described polypropylene mesh is unlatching shape.
Above-mentioned method, wherein, in described fibroin coating solution, the volumetric concentration of fibroin is 1 ~ 8%.Wherein, the fibroin that particularly preferably volumetric concentration is 4 ~ 6%.
The preparation method of above-mentioned silk-fibroin-coated polypropylene mesh, wherein, comprises the following steps:
Prepare silk protein solution: and polypropylene mesh more than 6 hours, is fully infiltrated, thereby on polypropylene mesh, applies one deck silk protein solution coating in being soaked in described silk protein solution;
By the polypropylene mesh that scribbles silk protein solution coating after infiltrating take out, dry, and be positioned over and in ethanol, soak after 20 ~ 40 minutes dryly, make silk-fibroin-coated polypropylene mesh.
Above-mentioned method, wherein, repeat polypropylene mesh be soaked in described silk protein solution, fully infiltrate with drying steps repeatedly after, more dried polypropylene mesh is soaked in alcoholic solution.
The application of above-mentioned silk-fibroin-coated polypropylene mesh, wherein, the mass concentration of described alcoholic solution is 60 ~ 90%.
Above-mentioned method, wherein, also comprises the preparation process of silk protein solution, the steps include: Bombyx bombycis to go successively after pupa, degumming process, dissolves; And the solution obtaining is carried out to deionized water dialysis, obtain regenerated silk protein solution; Dilute described regenerated silk protein solution, obtain volumetric concentration and be 1 ~ 8% fibroin coating solution.
Above-mentioned method, wherein, Bombyx bombycis goes the degumming process after pupa to comprise, first Bombyx bombycis is placed under 90 ~ 100 ℃ of conditions to 1 ~ 5% sodium bicarbonate and processes 30 ~ 90 minutes, takes out, and cleans with deionized water; After repetition said process 1 ~ 3 time, at 60 ~ 70 ℃, be dried 30 ~ 90 minutes, obtain boiled silk; Afterwards under 30 ~ 60 ℃ of conditions, boiled silk is processed 30 ~ 90 minutes being dissolved in the lithium-bromide solution of 7 ~ 10mol/L.
Above-mentioned method, wherein, in described Bombyx bombycis deionized water dialysis procedure, by being dissolved in silkworm silk solution in lithium bromide, to pour residual molecular weight into be in 10000 ~ 14000 fiber bag filter, with deionized water dialysis 2 ~ 3 days, thereby obtains fibroin coating solution.
Above-mentioned method, wherein, also comprises the cleaning course of putting into described silk protein solution polypropylene mesh before, and its step is first polypropylene mesh to be carried out, after ultrasonic cleaning, Cement Composite Treated by Plasma, obtaining clean polypropylene mesh.
An application for silk-fibroin-coated polypropylene mesh, wherein, the timbering material by the silk-fibroin-coated polypropylene mesh making as female pelvic reconstructive surgery.
Adopt the advantage of the preparation method of a kind of silk-fibroin-coated polypropylene mesh of the present invention to be:
1. the present invention is by the surface-coated fibroin coating at polypropylene mesh, thereby obtain silk-fibroin-coated polypropylene mesh, it utilizes the feature that polypropylene mesh porosity is high, the fine title deed for land of surrounding tissue energy enters growth on the one hand, strengthens the stretch-proof performance of repairing position; Utilize on the other hand the biocompatibility of regenerated silk albumen and promote the effect of fibroblastic growth, healing acceleration, reduces complication, and the present invention can be used for clinical female pelvic reconstructive surgery, effectively reduces the generation of Reconstruction infectious-related complication at the bottom of basin.
2. in the present invention, the preparation method process of silk-fibroin-coated polypropylene mesh is simple, does not relate to any poisonous and harmful reagent, and the follow-up use of medical patch is had no adverse effect, and fibroin to have source wide, price is low, preparation is simple, thereby greatly reduced medical cost, practical.
Accompanying drawing explanation
Fig. 1 is undressed polypropylene mesh stereoscan photograph;
Fig. 2 is the stereoscan photograph of silk-fibroin-coated polypropylene mesh;
Fig. 3 is for having silk-fibroin-coated polypropylene mesh group to implant to draw materials for 30 days Masson dyeing photo;
Fig. 4 is for implanting the Masson dyeing photo of drawing materials for 30 days without silk-fibroin-coated polypropylene mesh group;
Fig. 5 is for having silk-fibroin-coated polypropylene mesh group to implant to draw materials for 90 days Masson dyeing photo;
Fig. 6 is for implanting the Masson dyeing photo of drawing materials for 90 days without silk-fibroin-coated polypropylene mesh group;
Fig. 7 is that silk-fibroin-coated polypropylene mesh group is implanted 90 days transmission electron microscope photos of drawing materials;
Fig. 8 is for implanting 90 days transmission electron microscope photos of drawing materials without silk-fibroin-coated polypropylene mesh group;
Fig. 9 is the pull test curve chart of drawing materials for 30,60 and 90 days after silk-fibroin-coated polypropylene mesh group is implanted;
Figure 10 is the pull test curve chart of drawing materials for 30,60 and 90 days after implanting without silk-fibroin-coated polypropylene mesh group.
The specific embodiment
The invention provides a kind of silk-fibroin-coated polypropylene mesh and its preparation method and application.The fibroin coating that silk-fibroin-coated polypropylene mesh in the present invention is coated in described polypropylene mesh surface by the polypropylene mesh as support and employing fibroin coating solution forms.Wherein, after fibroin coating described in described polypropylene mesh surface-coated, the hole of described polypropylene mesh does not seal, and is still unlatching shape.
Wherein, in described fibroin coating solution, the volumetric concentration of fibroin is 1 ~ 8%, preferably 4 ~ 6%.
The preparation method of described silk-fibroin-coated polypropylene mesh, comprises the following steps:
Step 1, fibroin coating solution preparation: Bombyx bombycis is gone after pupa, degumming process successively, dissolve; And the solution obtaining is carried out to deionized water dialysis, obtain regenerated silk protein solution; Dilute described regenerated silk protein solution, obtain volumetric concentration and be 1 ~ 8% fibroin coating solution;
Step 2, silk-fibroin-coated polypropylene mesh preparation: clean polypropylene mesh is placed in to described fibroin coating solution, and fully infiltration is after 12 ~ 24 hours, and taking-up is dry; Repeat above-mentioned polypropylene mesh and infiltrate in fibroin coating solution, drying steps 4 ~ 6 times; Be positioned over afterwards in alcoholic solution and process 20 ~ 40 minutes, after drying, makes silk-fibroin-coated polypropylene mesh.
The present invention has also comprised the application of described fibroin polypropylene mesh, described fibroin polypropylene mesh is suitable as timbering material and is applied in female pelvic reconstructive surgery, described fibroin polypropylene mesh, can strengthen the stretch-proof performance of repairing position, but also there is good biocompatibility and the effect that promotes fibroblastic growth, contribute to reparation and the growth of tissue.
Below by specific embodiment, elaborate the present invention and advantage thereof, but concrete protection domain of the present invention is never confined to following embodiment.
Embodiment 1
Step 1: common polypropylene mesh is carried out to ultrasonic cleaning and Cement Composite Treated by Plasma, prepare clean polypropylene mesh.
Step 2: will purchase available pure silk protein solution and adopt deionized water to be diluted to concentration volumetric concentration 4 ~ 6%, and obtain regenerated silk protein solution.
Step 3: the clean polypropylene mesh obtaining in step 1 is inserted in the regenerated silk protein solution obtaining in step 2 and fully infiltrate, carry out the coating of regenerated silk protein solution on the surface of polypropylene mesh; After 15 hours, take out dry; Repeat after the regenerated silk protein solution coating procedure five times of above-mentioned polypropylene mesh, then place in 70% ethanol and process 20 ~ 40 minutes, obtain silk-fibroin-coated polypropylene mesh after dry.
Embodiment 2:
Step 1: common polypropylene mesh is carried out to ultrasonic cleaning and Cement Composite Treated by Plasma, prepare clean polypropylene mesh.
Step 2: fibroin coating solution preparation: Bombyx bombycis is removed to pupa, and the sodium bicarbonate with 1% ~ 5% is processed 30 ~ 90 minutes at 90-100 ℃, afterwards clean by rinsed with deionized water; Repeat above-mentioned cleaning of removing the Bombyx bombycis after pupa 1 ~ 3 time, to remove sericin completely; After to be cleaned, the Bombyx bombycis taking out after sericin is dried to 24 hours at 65 ℃, obtains boiled silk, and boiled silk is dissolved in the lithium-bromide solution of 7 ~ 10mol/L under 30 ~ 60 ℃ of conditions, to filter, remove undissolved coming unstuck; After 30 ~ 90 minutes, it is in 10000 ~ 14000 cellulose bag filter that the lithium-bromide solution of boiled silk is poured into residual molecular weight, and with deionized water dialysis 3 days, prepare regenerated silk protein solution, and the 5% regenerated silk protein solution obtaining with deionized water dilution.
Step 3: silk-fibroin-coated polypropylene mesh preparation: the clean polypropylene mesh obtaining in step 1 is inserted in 5% the regenerated silk protein solution obtaining in step 2 and fully infiltrated, carry out the coating of regenerated silk protein solution on the surface of polypropylene mesh; Dry after within 20 hours, taking out; Repeat the regenerated silk protein solution coating procedure four times of above-mentioned polypropylene mesh, then place in 90% ethanol and process 20 ~ 40 minutes, obtain silk-fibroin-coated polypropylene mesh after dry.
The silk-fibroin-coated polypropylene mesh that the present invention is made and common polypropylene mesh without silk rolling protein coating are observed, and contrast is found:
As shown in Figure 2, in Fig. 1, ganoid undressed polypropylene mesh, the surface-coated of silk-fibroin-coated polypropylene mesh of the present invention the uniform fibroin coating of one deck.And as shown in Figure 2, fibroin coating is full of polypropylene fibre surface and fiber junction, and polypropylene mesh hole do not seal, and is opening.
After prepared by described fibroin polypropylene mesh, after silk-fibroin-coated polypropylene mesh is encapsulated with needs cutting sizing, ethane via epoxyethane sterilization, standby.
Silk-fibroin-coated polypropylene mesh of the present invention, at polypropylene mesh, show to have applied one deck fibroin coating, it has retained the high porosity of polypropylene mesh, it utilizes the feature that polypropylene mesh porosity is high, the fine title deed for land of surrounding tissue energy enters growth on the one hand, strengthens the stretch-proof performance of repairing position; Utilize on the other hand the biocompatibility of regenerated silk albumen and promote the effect of fibroblastic growth, healing acceleration, reduces complication, and the present invention can be used for clinical female pelvic reconstructive surgery, effectively reduces the generation of Reconstruction infectious-related complication at the bottom of basin.And in preparation process, the present invention does not relate to any poisonous and harmful reagent, and the follow-up use of medical patch is had no adverse effect, and has guaranteed medical safe.
Below by experiment illustrate silk-fibroin-coated polypropylene mesh of the present invention with without fibroin polypropylene mesh as the medical beneficial effect of timbering material.
Experiment: rabbit abdominal-wall defect zoopery:
Experimental model: 6 of the female White Rabbits of clean level New Zealand, body weight 2.3 ~ 2.7kg, the about 2.5kg of average weight, rabbit is 3 months age.
6 new zealand white rabbits are divided into 3 groups at random, are respectively 30 days groups, 60 days groups, 90 days groups, 2 every group.According to implanting whether coated wire protein coating of polypropylene mesh, net sheet is divided into bis-groups of A, B, every group each 12.Wherein, embodiment of the present invention group (A group): have silk-fibroin-coated polypropylene mesh; Comparative example group (B group): without silk-fibroin-coated polypropylene mesh.
a,
the gross specimen that bis-groups of implantation net sheet different times of B are drawn materials is observed:
Net sheet is dead without laboratory animal after implanting, and two groups to be showed no intraperitoneal tolerant from the bulging of abdominal-wall defect place, and all net sheets intraperitoneal all do not occur and corrode and expose, without local abscess formation.Net sheet is sprawled smooth, before implanting, has no obvious distortion.Between two networking sheet surfaces and abdominal subcutaneous tissue, all form the connective tissue layer that thin layer is loose.
implant the HE dyeing observation that net sheet different time is drawn materials for two groups:(HE dyeing: hematoxylin-eosin staining method, hematoxylin-eosin staining):
(1), when net sheet is implanted 30 days, visible embedded material has more inflammatory cell to assemble around.Along with the increase of Implantation Time, net sheet around inflammatory cell quantity obviously reduces; Fibroblast quantity increases, and form is also gradually ripe; Collagen secretion increases.Fibril without fibroin coating networking sheet is all shown in more inflammatory response cell, plasma cell around, and macrophage amount is few compared with inflammatory cell, simultaneously visible minute quantity multinucleated giant cell.Have even can be in net sheet peripheral vessels the leukocyte of volume.Have inflammatory reaction and the fiberization of fibroin coated mesh sheet group (A group) all lighter, the new vessels generation around of net sheet is obvious, and macrophage quantity is compared with many without fibroin coated mesh sheet.
(2) when net sheet is implanted 60 days, two networking sheets are visible inflammatory response cell still around, but obviously reduces while implanting 30 days, and macrophage quantity also reduces, and visible a small amount of eosinophilic granulocyte and basophilic granulocyte; And net sheet increases the weight of when around fiberization is implanted 30 days.Part is without fibroin coated mesh sheet (B group) more visible lymphocytic infiltrations still around.
(3), when net sheet is implanted 90 days, each networking sheet almost loses inflammatory response cell around; Macrophage still exists, but quantity further reduces.Around fiberization is light without fibroin coated mesh sheet (B group) fibroin coated mesh sheet (A group).Net sheet peripheral vesselsization reaction is more obvious when implanting 30 days and 60 days.Now, all net sheets all show good histocompatibility, net sheet and surrounding tissue good knitting.
implant the Masson dyeing observation that net sheet different time is drawn materials for two groups:
Two groups of collagen stainings (Masson dyeing) of implanting net sheet show: for two networking sheets, when A group fibroin coated mesh sheet of the present invention (Fig. 3) and B group contrast networking sheet (Fig. 4) are implanted 30 days, after two networking sheets are implanted, the fibril of polypropylene mesh around more inflammatory cell is assembled, and is with a small amount of sparse newborn collagen tissue outward.Net sheet is implanted (Fig. 5 after 90 days, Fig. 6), the fibril that has a silk-fibroin-coated polypropylene mesh (A group) is rarely seen a small amount of inflammatory response cell around, macrophage is also less, fibril around visible obviously collagen tissue holds, net sheet and surrounding tissue good knitting, have more inflammatory response cell and macrophage around and surround without obvious collagen tissue without silk-fibroin-coated polypropylene mesh (B group), and itself and surrounding tissue amalgamation are poor.Therefore, the present invention has fibroin coated mesh sheet newborn collagen quantity around compared with many without fibroin coated mesh sheet, and it is better with the biocompatibility of tissue.
implant the transmission electron microscope observing that net sheet different time is drawn materials for two groups:
When net sheet is implanted 30 days, net sheet around more inflammatory cell holds, and fibrosis forms not obvious.Inflammatory cell when net sheet is implanted 60 days and 90 days reduces before, comparison diagram 7 and Fig. 8, and than the net sheet without fibroin coating, embodiment of the present invention silk-fibroin-coated polypropylene mesh periphery fiberization is stronger, and fibroblast is arranged orderly.
Fig. 9 and Figure 10 are embodiment group and comparative example networking sheet 30 days, 60 days, and 90 days tensile stress-strain figures, vertical coordinate represents tensile stress at break (MPa), the abscissa elongation strain (%) that represents to rupture.
In figure as seen along with Implantation Time extends, tensile stress at break, fracture elongation strain, and disconnected when de-tensile stress totally present increase tendency.The silk-fibroin-coated polypropylene mesh of embodiment group the tensile stress at break of 30 days, 60 days, 90 days all higher than the comparative example group of the same period without silk-fibroin-coated polypropylene mesh.The trend that the fracture elongation strain parameter of silk-fibroin-coated polypropylene mesh of the present invention increases with Implantation Time prolongation is obvious, not obvious at the increase tendency without silk-fibroin-coated polypropylene mesh.
From respectively organizing above experimental diagrams, show, silk-fibroin-coated polypropylene mesh of the present invention, utilizes the feature that polypropylene sticking patch porosity is high, surrounding tissue is cut growth well on the one hand, can strengthen the stretch-proof performance of repairing position; Utilize on the other hand the biocompatibility of regenerated silk albumen, increase the biocompatibility of implant and tissue, reduce the generation of post-operative complication.
The present invention shows through results of animal, the present invention is applied to department of obstetrics and gynecology female pelvic reconstruction operations and surgery abdominal hernia repairing operation as timbering material, the polypropylene mesh of fibroin coating can reduce the reaction of implant site Earlier period of inflammation and fibrosis, reduce tissue adhesion, increase biocompatibility, reduce the generation of net sheet related complication, and silk-fibroin-coated polypropylene mesh has relatively good tensile stress and elongation strain is answered, and is conducive to the growth of female pelvic tissue.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (7)
1. a preparation method for silk-fibroin-coated polypropylene mesh, is characterized in that, comprises the following steps:
Prepare silk protein solution; The steps include: Bombyx bombycis to go successively after pupa, degumming process, dissolve; And the solution obtaining is carried out to deionized water dialysis, obtain regenerated silk protein solution; Dilute described regenerated silk protein solution, obtain volumetric concentration and be 1～8% silk protein solution;
Wherein, Bombyx bombycis goes the degumming process after pupa to comprise: first Bombyx bombycis is placed under 90～100 ℃ of conditions to 1～5% sodium bicarbonate and processes 30～90 minutes, take out, and clean with deionized water; After repetition said process 1～3 time, at 60～70 ℃, be dried 30～90 minutes, obtain boiled silk; Afterwards under 30～60 ℃ of conditions, boiled silk is dissolved in the lithium-bromide solution of 7～10mol/L and processes 30～90 minutes;
Polypropylene mesh is soaked in described silk protein solution more than 6 hours, fully infiltrates, thereby on polypropylene mesh, apply one deck fibroin coating;
The polypropylene mesh that scribbles fibroin coating after infiltrating is taken out, is dried, repeat polypropylene mesh be soaked in described silk protein solution, fully infiltrate with drying steps 4-6 time after, be positioned in ethanol and soak after 20～40 minutes and be dried, make silk-fibroin-coated polypropylene mesh.
2. a method of claim 1, is characterized in that, the mass concentration of described alcoholic solution is 60～90%.
3. method according to claim 1, it is characterized in that, in described deionized water dialysis procedure, by being dissolved in silkworm silk solution in lithium bromide, to pour molecular cut off into be in 10000～14000 fiber bag filter, with deionized water dialysis 2～3 days, thereby obtain regenerated silk protein coating solution.
4. method according to claim 1, it is characterized in that, also comprise the cleaning course of putting into described silk protein solution polypropylene mesh before, its step is, first polypropylene mesh is carried out, after ultrasonic cleaning, Cement Composite Treated by Plasma, obtaining clean polypropylene mesh.
5. a silk-fibroin-coated polypropylene mesh for the timbering material for female pelvic reconstructive surgery that as claimed in claim 1 prepared by method, is characterized in that, comprises the polypropylene mesh as support; On described polypropylene mesh surface, adopt fibroin coating solution parcel one deck fibroin coating; And the hole of described polypropylene mesh is unlatching shape.
6. silk-fibroin-coated polypropylene mesh according to claim 5, is characterized in that, in described fibroin coating solution, the volumetric concentration of fibroin is 1～8%.
7. an application for silk-fibroin-coated polypropylene mesh as claimed in claim 5, is characterized in that, described silk-fibroin-coated polypropylene mesh is for making the timbering material of female pelvic reconstructive surgery.
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| CN114392396B (en) * | 2022-01-19 | 2022-10-28 | 福州市第二医院（福建省福州中西医结合医院、福州市职业病医院） | Rotator cuff patch based on silk fibroin gel and preparation method thereof |
| CN114366850A (en) * | 2022-01-19 | 2022-04-19 | 复旦大学附属华山医院 | Artificial rotator cuff patch based on drug-loaded silk protein and preparation method thereof |
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